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equine markers genes. polymorphism for group-specific component (gc).polymorphism of equine gc protein was demonstrated by immunofixation electrophoresis with a goat anti-human gc antibody. three different phenotypes, f, fs and s, were found. family data supported the genetic theory of two autosomal codominant alleles, gcf and gcs. both alleles occurred in standardbred, thoroughbred and arabian horses and in shetland ponies. a frequency of 0.23 for gcs in the american standardbred horse indicates the system should be useful for problems of identification and pare ...197884540
solid phase indirect radioimmunoassays for the rapid diagnosis of sindbis virus antigen.indirect radioimmunoassays have been developed for the rapid detection of sindbis virus. dilutions of sindbis virus from tissue culture fluids have been immobilized and allowed to react with rabbit anti-sindbis virus antibodies. the bound antibodies were assayed either by 125i-labelled anti-rabbit igg-antibodies or alternatively by addition of human complement and 125i-labelled anti-human c1q antibodies or 125i-labelled protein a.197884855
observations on the m-wave and the cnv in the squirrel monkey.a typical cnv paradigm, with food as reward, evokes in the squirrel monkey's post-arcuate and post-central cortices both m-waves in response to the cues and what appears to be a cnv in the interstimulus interval. both wave forms appear to be generated locally in cortex and to be more closely related temporally to the cues than to the animal's behavioral response. the cnv, like the m-wave, appears to reflect the animal's level of interest in obtaining the reward. it is smaller when the animal mis ...197985525
[perichordal necroses. contribution to the understanding of the spinal column morphogenesis].in 12--15 days old rat embryos and 12 days old mouse embryos vitally stained by acridin orange, a perichordal necrosis appears, showing metameric intensifications at the level of the dense sclerotomites. similar aspects were detected in serially sectioned rabbit and human embryos (6--7 mm c.r. length). the authors correlate the necrotic zones mentioned with the inductive functions of the notochord.197886154
immune suppression in vivo with antigen-modified syngeneic cells. ii. t cell-mediated nonresponsiveness to fowl gamma-globulin.intravenous administration of syngeneic spleen cells coupled with the palmitoyl derivative of fowl gammma-globulin (p-f gamma g) results in a profound state of f gamma g-specific tolerance in c57bl/6 mice. administration of p-f gamma g coupled syngeneic cells specifically reduces both the primary and secondary hapten and carrier-specific pfc responses to tnp-f gamma g. since the haptenic response is affected, the tolerance functions at the level of the f gamma g-specific helper t cell. as few as ...197987447
hippocampal eeg and behaviour in dog. ii. hippocampal eeg correlates with elementary motor acts.a positive correlation has been shown between the speed of forced stepping on a conveyor belt and the amplitude and frequency of the concomitant hippocampal eeg. significant modulation in the spectral properties of the dog's hippocampal eeg has been found in relation to 3 elementary motor acts: stepping, respiratory movements and a reflex movement in reaction to linear acceleration of the animal. the findings support the idea that within a certain experimental situation the hippocampal eeg refle ...197988345
effects of arachidonic acid and its metabolites on antigen-induced histamine release from human basophils in vitro. 197990097
[the appearance of penicillin resistant gonococci. clinical, genetic and epidemiologic problems]. 197896408
local cerebral energy metabolism: its relationships to local functional activity and blood flow.the results of studies with the [14c] deoxyglucose technique unequivocally establish that local functional activity in cerebral tissues is, as in other tissues, closely coupled to the local rate of energy metabolism. stimulation of local functional activity increases the local rate of glucose utilization; reduced functional activity depresses it. local cerebral blood flow is normally distributed among the cerebral structures in almost exact proportion to their rates of glucose utilization and it ...197897062
gonorrhea detection in posthysterectomy patients.a retrospective study of women examined at the venereal disease clinic after hysterectomy was made to determine which culture site was most likely to show anogenital neisseria gonorrhoeae infection when the cervix was absent. seventeen of 104 such patients were culture positive for n gonorrhoeae. rates of positivity for our culture sites were as follows: urethral, 17/17 (100%); vaginal, 7/17 (41%); and rectal, 2/17 (12%).197898647
development of leucocytozoon caulleryi in chick embryos infected by biting of culicoides arakawae through shell membrane.chick embryos were infected with leucocytozoon caulleryi by biting of the midge, culicoides arakawae, through the shell membrane. schizonts of l. caulleryi were detected in the chorioallantoic membrane and most of the internal organs of embryos and of chicks after hatching. the development of schizonts was slower in embryos than in chickens. soluble antigens of l. caulleryi were demonstrated by the precipitation test in the allantoic fluid and blood from the embryos and chicks. no erythrocytic s ...197899671
[cannabis].cannabis produces a resin, the psychotropic effects of which are known since the high antiquity. it induces euphoria, a feeling of pleasant dreaminess. nevertheless, psychiatric disturbances such as psychomotor deficiency states or acute oniroïd states were described in heavy users countries. these psychiatric disorders put the accent on the starting or catalysing role of cannabis on the premorbid personality. the experimental studies of cannabis on the premorbid personality. the experimental st ...1978100034
[cadmium in the kidneys of slaughtered animals--hazardous for human health?]. 1978101772
dissociation of autonomic and cognitive effects of thc in man.intravenous thc, 30--44.8 microgram/kg, was administered to four subjects. each received thc on four occasions preceded by either i.v. saline, 0.04 mg/kg atropine sulfate, 0.2 mg/kg propranolol, or both drugs together. heart rates, subjective intoxication and symptom ratings, time productions, and eeg activity were measured. in the absence of autonomic blocking drugs, thc produced characteristic tachycardia, subjective intoxication, and eeg effects. after combined autonomic blockade, thc had no ...1979108734
biologic activity of soluble collagen-anticollagen immune complexes as demonstrated by passive cutaneous anaphylaxis.collagen-anticollagen immune complexes were prepared by gel filtration. complex formation was chemically and serologically demonstrated. complexes eluting in gammam- and gammag-peaks of antigen-antiserum mixture showed strong positive reactions in pca experiments performed with 20 guinea pigs. control preparations of gammam- and gammag-peaks of antiserum devoid of antigen gave significantly lower pca-response. the positive reaction of control preparations can be explained in part by a certain de ...1975127469
[effect of para-chlorophenylalanine and 5-hydroxytryptophan on the caudate nucleus in cats].in unrestrained cats parachlorophenylalanine, an inhibitor of serotonin synthesis, increased the thresholds of the contraversive reaction and arrest reaction accompanied by spindle-waves in the sensory-motor cortex in the stimulation of the rostro-ventral areas of the caudate nucleus. the reactions evoked from the dorso-medial areas of the head and the body of the nucleus changed differently. on the contrary, 5-hydroxytryptophane, serotonin precursor, increased most of the indices of the caudate ...1978149572
experimental spongy degeneration of the white matter induced by 6-aminonicotinamide intoxication.spongy state and degeneration of the white matter in the 6-aminonicotinamide intoxicated rats were studied neuropathologically and electromicroscopically. young albino rats, four weeks of age, received 0.1% of 6-aminonicotinamide solution intraperitoneally as a single dose of 10 mg/kg of body weight. four hours after the administration, paralysis of the hind limbs occurred in the rats. neuropathological investigations revealed a characteristic spongy and degenerative change of white matter as we ...1978149717
effectiveness of orally administered bordetella pertussis vaccine in mice.oral administration of killed bordetella pertussis organisms to mice results in increased resistance to an intracerebral infection with virulent b. pertussis cells. the rate of survival is dependent on the dose of antigen. but besides specific systemic immunity, which is persistent over a long period, also transient non-specific resistance is increased. these effects are evidently induced without penetration of bacterial substances into the circulation.1976182602
adenovirus of ring-necked pheasants: purification and partial characterization of marble spleen disease virus.a method for purification of marble spleen disease virus (msdv) from the spleens of infected turkeys and pheasants is described. it combines chloroform or fluorocarbon extraction with subsequent purification by centrifugation on a cushion of cesium chloride (cscl). further purification of msdv was accomplished with a cscl equilibrium density gradient. a viral buoyant density of approximately 1.32 to 1.33 g/cm3 was determined. negative-stain electron microscopy revealed that virus isopycnically b ...1977193796
radiometric detection of haemophilus in simulated blood cultures.the radiometric detection of various haemophilus species was studied in simulated blood cultures and in blood culture media without added blood, but supplemented with nicotinamide-adenine dinucleotide (nad) or hemin, or both. haemophilus aphrophilus was the only species studied that was radiometrically detectable without added blood or nad. all other species studied (haemophilus influenzae, haemophilus parainfluenzae, haemophilus haemolyticus, and haemophilus parahaemolyticus) required either ad ...1979218444
[a case of glomerulonephritis and subacute endocarditis associated to hemophilus aphrophilus septiciemia].a report is presented on the findings in what is to our knowledge the first described case of glomerulonephritis resulting from haemophilus aphrophilus endocarditis. after an insidious onset, serious renal failure developed which subsided with antibiotic therapy. cardiac damage was minimal and the patient recovered his usual state of health, an outcome which has not been so satisfactory in other reported cases of endocarditis due to haemophilus aphrophilus. the case is discussed with reference t ...1978245746
identification and cloning of the chloroplast gene coding for the large subunit of ribulose-1,5-bisphosphate carboxylase from chlamydomonas reinhardi.mrna coding for the large subunit (ls) of ribulose-1,5-bisphosphate carboxylase [3-phospho-d-glycerate carboxy-lyase (dimerizing), ec 4.1.1.39] from chlamydomonas reinhardi has been isolated from small polyribosomes immunoadsorbed to column-bound anti-ls antibody. 32p-labeled ls mrna was used as a hybridization probe to detect ls genes. the probe hybridized to c. reinhardi chloroplast dna and at hybridization saturation revealed that there are approximately 75 ls genes per chloroplast. when chlo ...1977269382
isolation of actinobacillus actinomycetemcomitans and haemophilus aphrophilus at auckland hospital.actinobacillus actinomycetemcomitans and haemophilus aphrophilus were each isolated three times at auckland hospital, over a one year period. relevant clinical particulars of the major cases are given, and problems of isolation, identity and treatment discussed.1979289008
neonatal haemophilus aphrophilus meningitis.case report of a neonatal meningitis due to haemophilus influenzae. the treatment with ampicillin was ineffective because of insensitivity. this case demonstrates the importance of a proper bacteriological identification in order to avoid ineffective or delayed treatment.1977300734
bacteriology of abscesses of the central nervous system: a multicentre prospective study.pus from 46 patients with abscesses of the central nervous system (cns) was examined for bacteria; bacteria were found in all patients. streptococci were isolated from 36 patients and most isolates were streptococcus milleri, lancefield group f, ottens and winkler type o iii. staphylococci were isolated from nine patients, organisms of the bacteroides group from 11, proteus spp from seven, klebsiella aerogenes from one, and haemophilus aphrophilus from one. pure cultures predominated over mixed ...1977336141
haemophilus aphrophilus: a microbiologic and clinical review and report of 42 cases. 1978351324
killing of tumor cells in vitro by macrophages from mice treated with synthetic dehydrodipeptides.treatment of nmri mice i.p. with dehydrodipeptides [acetyldehydro-3-(2-thienyl)alanyltyrosine (si); acetyldehydro-3-(2-furyl)alanyltyrosine (sii)] rendered macrophages cytolytic for several tumor cells in vitro. normal peritoneal mouse macrophages from untreated mice not given injections of the peptides or from control mice given injections of phosphate-buffered saline were not cytotoxic. moreover, supernatants from these in vivo-activated mouse peritoneal macrophages significantly increased the ...1979427817
[study of heat denaturation of rhodopsin in the external retinal rod segments of cattle by infrared spectroscopy]. 1979476183
chlamydial genital infections: a growing problem. 1979478491
haemophilus aphrophilus. 1979508152
haemophilus aphrophilus cholecystitis.cultures from the gallbladder and blood of a 60-year-old man with acute cholecystitis grew haemophilus aphrophilus. this organism, an unusual isolate in clinical specimens, is most frequently seen in patients with either endocarditis or brain abscesses. haemophilus aphrophilus may be distinguished from eikenella corrodens and actinobacillus actinomycetemcomitans on the basis of colonial morphology and the biochemical tests for oxidase and catalase production and fermentation of lactose, sucrose, ...1978637050
haemophilus paraphrophilus isolated from endocarditis.isolation and microbiological features of a haemophilus paraphrophilus strain which caused a subacute bacterial endocarditis are described. the isolate conformed to the first description by zinnemann et al., except that it was sensitive to ampicillin.1978696435
mspi, an isoschizomer of hpaii which cleaves both unmethylated and methylated hpaii sites.the cleavage of dna by restriction endonucleases hpaii and hapii is prevented by the presence of a 5-methyl group at the internal c residue of its recognition sequence ccgg. mspi, an isoschizomer of hpaii available from new england biolabs, cleaves dna irrespective of the presence of a methyl group at this position. this enzyme cleaves dna from haemophilus parainfluenzae and haemophilus aphrophilus readily while hpaii and hapii cannot degrade these dnas. practically all hpaii sites in mammalian ...1978704354
necrotizing fasciitis associated with haemophilus aphrophilus. report of a case.necrotizing fasciitis, a serious infection requiring prompt antibiotic and surgical therapy, previously has not been associated with haemophilus aphrophilus. we report a case of necrotizing fasciitis associated with methylphenidate abuse from which h aphrophilus was isolated. surgical and antibiotic therapy based on in vitro susceptibility testing resulted in eradication of the infection.1978718326
case report. cardiobacterium hominis: review of prior infections and report of endocarditis on a fascia lata prosthetic heart valve.the clinical spectrum of disease caused by cardiobacterium hominis, a small, gram-negative coccobacillus, is little known to physicians. from analysis of a new case of c hominis endocarditis on a fascia lata aortic valve, and from review of all previous reports of infection due to this microorganism, a remarkably consistent clinical picture emerged. c hominis only causes endocarditis, affects middle-aged individuals with structurally abnormal, left-sided cardiac valves, and follows a subacute co ...1978727215
haemophilus aphrophilus endocarditis in pregnancy.the clinical and microbiological features of a case of haemophilus aphrophilus endocarditis in pregnancy are described. the complicating effect of pregnancy on treatment and the difficulties in identifying the organism in the laboratory are discussed.1976789409
the agglutination reactions of haemophilus paraphrophilus and h. paraphrohaemolyticus, and some observations on the agglutination of h. aphrophilus and h. haemoglobinophilus (h. canis).agglutination tests were used to study the surface antigens of two recently described species of the genus haemophilus, h. paraphrophilus and h. paraphrohaemolyticus, and their antigenic relationship to other members of the genus. the results obtained with a few strains of h. haemoglobinophilus and h. aphrophilus are also reported. the species h. paraphrophilus appears to be homogeneous; no major cross-reactions were observed. the species h. paraphrohaemolyticus contains at least three serotypes ...1975805245
haemophilus aphrophilus in brain abscess: a case report. 1976972294
haemophilus paraphrophilus endocarditis in a prolapsed mitral valve.a case of haemophilus paraphrophilus endocarditis successfully treated with ampicillin is described. the patient, a 24-year-old woman, had a prolapsed mitral valve. the organism was initally misidentified as h. parainfluenzae, which it closely resembles. h. paraphrophilus is distinguished by its requirement of 10% co2 for growth on nacl-free medium and its inability to ferment xylose.1976983999
binding of human growth hormone to human peripheral lymphocytes. 19761002110
ampicillin-resistant haemophilus paraphrophilus laryngo-epiglottitis.a case of life-threatening laryngo-epiglottitis is reported, caused by ampicillin-resistant haemophilus paraphrophilus. clinicians and microbiologists should be aware of a beta-lactamase-mediated resistance among haemophilus species other than h. influenzae.19761049588
isolation of haemophilus aphrophilus from an adult with acute leukemia.during the course of treatment for acute myelocytic leukemia, haemophilus aphrophilus was isolated from eight blood cultures associated with the clinical picture of bacterial endocarditis.19761067263
cardiobacterium homonis endocarditis. characterization of the unusual organisms and review of the literature.a case of subacute bacterial endocarditis in which cardiobacterium hominis was isolated from the blood of a 55-year old woman who had rheumatic heart disease is reported. a survey of the literature revealed very few reports in which this organism has been implicated in human lesions. the colonies grew after 48 hours of incubation in a candle jar. they were small, convex, nonhemolytic, and oxidase-positive. the indole reaction was positive, the catalase and nitrate reactions were negative, and ac ...19751089355
the antiarrhythmic and hemodynamic actions of alpha, alpha-dimethyl-4-(alpha, alpha, beta, beta-tetrafluorophenethyl) benzylamine (mk-251).alpha,alpha-dimethyl-4-(alpha,alpha,beta,beta-tetrafluorophenethyl)benzylamine (mk-251) has been found to prevent certain types of experimentally induced ventricular arrhythmias and at maximally effective doses possesses substantial hemodynamic safety in contrast to standard antiarrhythmic agents. mk-251 prevented or modified ventricular arrhythmias produced by injection of tetrafluorohexachlorobutane into the coronary artery of dogs and baboons. in dogs, the dose estimated to prevent 80% of the ...19751151767
[case of d/e translocation 45, xx, 13--, 18--, t(18q+, 13--)]. 19751234109
quantitative correlations between chemical structure and affinity for acetylcholine receptors.the affinity constants (log k, pa2) of 128 quaternary ammonium compounds belonging to several different series have been correlated linearly with the hydrophobicity (pir) constant, the dipole moment (mur), and the number of hydroxyl groups (noh) of the side chain; the dependence on the hydrophobicity constant of the quaternary ammonium head (pi-n identical to) is shown to be parabolic. a correlation coefficient of 0.96 is obtained for all the compounds using only 4 independent variables (6 terms ...19761248503
rapid identification of actinobacillus actinomycetemcomitans based on analysis of 23s ribosomal rna.actinobacillus actinomycetemcomitans is a key microorganism in the pathogenesis of several different forms of periodontal diseases. identification of this bacterium from clinical specimens may often be complicated by the fact that the colony morphology on tsbv selective medium closely resembles that of haemophilus aphrophilus and a key differentiating characteristic, catalase reaction, may be variable. recent genetic studies have shown that the 23s ribosomal rna molecule is split into two smalle ...19921284398
intra-injector methylation of free fatty acids from aerobically and anaerobically cultured actinobacillus actinomycetemcomitans and haemophilus aphrophilus.free fatty acids from the type strains of anaerobically and aerobically broth-cultured actinobacillus actinomycetemcomitans and haemophilus aphrophilus cells were soxhlet-extracted with hexane. the fatty acids were identified and quantified by gas chromatography and gas chromatography-mass spectrometry after intra-injector derivation with trimethylanilinium hydroxide. this derivatization method, which we propose as suitable for routine use in clinical microbiology, is fast, accurate and sensitiv ...19921400721
hepatobiliary infections caused by haemophilus species.haemophilus species are rarely associated with hepatobiliary infections. we report a case of hepatic abscess caused by haemophilus paraphrophilus and review the english-language literature for reports of infections of the liver and biliary system caused by haemophilus species. most patients identified had predisposing conditions. the pathogenesis of hepatobiliary infections due to haemophilus species may involve ascending spread from the gastrointestinal tract or hematogenous seeding following o ...19921420691
[the spectrum of pathogens in positive blood cultures--tyrol 1991].in a recent study by the centers for disease control (cdc) it was noted that there had been a resurgence of gram-positive bacteremia together with an increase in fungemia. this reported trend is confirmed by data from the austrian tirol. in 1991 1,750 out of 13,679 specimens (12.8%) yielded bacterial or fungal growth, accounting for 1,248 cases of "bacteremia"; no decision was made about the clinical significance of the culture isolates. we consider laboratory reports of blood isolates to be fai ...19921481545
[necrotizing fasciitis caused by haemophilus paraphrophilus]. 19921489801
effect of guinea-pig conceptus and its secretions on endometrial prostaglandin output.the co-culture of day-15 guinea-pig conceptuses or day-15 pregnant guinea-pig endometrium with day-15 non-pregnant guinea-pig endometrium had no inhibitory effect on pgf2 alpha output from the non-pregnant endometrium. unpurified proteins secreted by the day-15 guinea-pig conceptuses, or these proteins purified by blue sepharose cl-6b and ion-exchange column chromatography also had no inhibitory effect on pgf2 alpha output from day-15 non-pregnant guinea-pig endometrium cultured in vitro. howeve ...19921492104
isolation and characterization of a newly identified haemophilus species from cats: "haemophilus felis".a gram-negative coccobacillus was isolated from the lower respiratory tract of a cat with chronic obstructive pulmonary disease. the isolate required co2 and v factor for growth and was initially identified as haemophilus paraphrophilus on the basis of its nutritional requirements, colony morphology, and some biochemical tests. because of the host specificity of haemophilus species and discrepancies in catalase, oxidase, and hemolytic activities, additional testing was done. extensive biochemica ...19921500518
protein kinase c activity affects glucose-induced oscillations in cytoplasmic free ca2+ in the pancreatic b-cell.acute stimulation of protein kinase c (pkc) inhibited glucose-induced slow oscillations in cytoplasmic free ca(2+)-concentration, [ca2+]i, in mouse pancreatic b-cells. in pkc-depleted cells glucose induced rapid transients in [ca2+]i, lasting for approximately 10 s, superimposed on the slow oscillations in [ca2+]i. it was demonstrated that the transients did not occur in the absence of extracellular ca2+. each transient typically was preceded by a slow increase in [ca2+]i, representing the risin ...19921592121
transforming growth factor beta induces myoblast differentiation in the presence of mitogens.transforming growth factor beta 1 (tgf-beta 1) added to l6e9 rat skeletal myoblasts in mitogen-rich medium induces a rapid decrease in c-myc expression and delays progression through the g1 phase of the cell cycle. this growth inhibitory response is followed by cell commitment to terminal differentiation with elevated expression of myogenin muscle determination genes, induction of muscle-specific proteins, and formation of multinucleated myotubes. these results suggest that tgf-beta 1 may act as ...19921594628
treatment of haemophilus aphrophilus endocarditis with ciprofloxacin.a patient with haemophilus aphrophilus endocarditis was successfully treated with ciprofloxacin. the response to treatment with cefotaxime and netilmicin for 12 days was poor but was satisfactory to a 6 weeks' course of ciprofloxacin.19921602151
sensitivity of actinobacillus actinomycetemcomitans and haemophilus aphrophilus to oxidative killing.we examined the killing of actinobacillus actinomycetemcomitans and haemophilus aphrophilus by oxygen metabolites generated by the xanthine-xanthine oxidase (x-xo) system. this system generates a mixture of oxidants, including superoxide radical, hydrogen peroxide, hydroxyl radical, and possibly singlet oxygen. differential sensitivity to the x-xo system was observed among strains of a. actinomycetemcomitans; notably, 2 catalase-deficient strains and 2 strains representative of serotypes b and c ...19911668250
increased [met]enkephalin and decreased substance p in spinal cord following thermal injury to one limb.thermal injury to one hind limb of rats was induced by immersion into water at 62 degrees c. both a mild (15 s) or severe (30 s) lesion caused inflammation of the limb when observed 24 h later; but at this time the animals used the injured limb when they walked. animals with a severe lesion of the injured limb subsequently withdrew it from use when walking. limb withdrawal did not occur following a mild lesion. at 24 h following the lesion, lumbar spinal cord levels of [met]enkephalin, as measur ...19901700332
application of multivariate analyses of enzymic data to classification of members of the actinobacillus-haemophilus-pasteurella group.outer membrane vesicles and fragments from actinobacillus actinomycetemcomitans, actinobacillus lignieresii, actinobacillus ureae, haemophilus aphrophilus, haemophilus paraphrophilus, haemophilus influenzae, haemophilus parainfluenzae, pasteurella haemolytica, and pasteurella multocida were isolated and examined semiquantitatively for 19 enzyme activities by using the api zym micromethod. the enzyme contents of vesicles and fragments were compared with the enzyme contents of whole cells of the s ...19921736960
gentamicin-collagen sponge for local applications. 10 cases of chronic osteomyelitis followed for 1 year.a bovine collagen sponge containing gentamicin was applied locally in 10 patients with chronic osteomyelitis who were then followed for 1 year postoperatively. no adverse effects or recurrence of infections was observed. the release of gentamicin from the sponge was more complete and rapid than has been reported with polymethyl methacrylate (pmma) beads. compared with previous reports on pmma beads, a much higher gentamicin concentration in the wound exudate can be expected after implantation of ...19911767654
recombinant human insulin-like growth factor i: effects in normal subjects and implications for use in patients. 19911767745
the biting rate of triatoma infestans in argentina.1. the daily proportion of fed individuals in a population of the reduviid bug triatoma infestans (klug), maintained under natural climatic conditions in experimental chicken houses in central argentina, was estimated from the proportion of bugs retaining colourless urine in the rectum. 2. from the estimates of feeding frequency throughout a 1 year period, it was shown that temperature has a dominant effect on biting rate, but density-dependent effects became apparent during the warmest months. ...19911768924
infective endocarditis with haemophilus aphrophilus. 19911794912
intracranial abscesses in odense hospital. survey of bacteriology, epidemiology, and treatment with antibiotics, 1963-1989.fifty-nine patients were operated or punctured in 60 incidents of brain abscess from 1963-1989, twice as many in men as in women. the number of cases tripled in 1980 to an incidence of 3.6 per million inhabitants per year, supposedly due to the advent of computerized tomography. simultaneously, the aetiology changed from staphylococci and gram negative rods to dominance of streptococci and haemophilus aphrophilus. apart from temporal abscesses, there was no correlation between localisation in th ...19911802627
cell surface hydrophobicity and electrokinetic potential of actinobacillus actinomycetemcomitans and haemophilus aphrophilus.laboratory strains and fresh isolates of actinobacillus actinomycetemcomitans and haemophilus aphrophilus were examined for cell surface hydrophobicity and for electrokinetic properties under different experimental conditions. fresh isolates of a. actinomycetemcomitans and h. aphrophilus were hydrophobic. laboratory strains of a. actinomycetemcomitans were 20-30% less hydrophobic than fresh isolates. no difference was observed between laboratory and fresh isolates of h. aphrophilus. the ph of th ...19911812465
peritoneal macrophage response: an in vivo model for the study of synthetic materials.this study was designed to validate an in vivo model in rat to study cell-implant interactions. pieces of dacron and goretex and polydimethylsiloxane (reference material) precoated or not were implanted in the peritoneal cavity. in some cases the peritoneal cells were stimulated before the implantation. macrophage phagocytosis and fibrinocoagulolytic activities were investigated in parallel with morphological studies 6 h after implantation. a graded cell response related to the different situati ...19911831674
peritoneal macrophage response: an in vivo model for the study of synthetic materials.this study was designed to validate an in vivo model in rat to study cell-implant interactions. pieces of dacron and goretex and polydimethylsiloxane (reference material) precoated or not were implanted in the peritoneal cavity. in some cases the peritoneal cells were stimulated before the implantation. macrophage phagocytosis and fibrinocoagulolytic activities were investigated in parallel with morphological studies 6 h after implantation. a graded cell response related to the different situati ...19911831674
use of synthetic oligonucleotide dna probes for the identification of different strains of fusobacterium nucleatum.the ability of three different oligonucleotide probes to identify different oral strains of fusobacterium nucleatum was tested. probes 119 and 214 were designed on the basis of known amino acid sequences from the n-terminal end of an outer membrane protein of f. nucleatum strain fev1. probe h 2.1, was a randomly cloned chromosomal dna fragment of 2.1 kbp. the specificities of the probes were examined by testing each of them against a panel of five other species of fusobacterium (six strains), or ...19911837056
application of quadferm+ for the identification of fastidious gram-positive and gram-negative bacilli.the 2-hr quadferm+ kit (qf) (analytab products, plainview, ny) was compared with conventional tube tests for the identification of the hacek bacteria (haemophilus aphrophilus, actinobacillus actinomycetemcomitans, cardiobacterium hominis, eikenella corrodens, kingella kingae), other haemophilus and kingella spp., capnocytophaga, corynbacterium, and moraxella spp. test results were identical for 296 comparisons with 74 isolates (74%). in the remaining 104 comparisons for 26 isolates, 50 reactions ...19911873978
[haemophilus aphrophilus--a rare endocarditis pathogen]. 19911921900
some unusual members of the family pasteurellaceae isolated from human sources--phenotypic features and genomic relationships.twelve human isolates exhibiting the properties of the family pasteurellaceae but phenotypically deviating from established species, or resembling species of animal origin that are only rarely reported to occur in human materials, were checked for their identities by dna-dna hybridization. the collection consisted of one strain of actinobacillus lignieresii, two strains of actinobacillus hominis (mannose-positive), two hitherto undescribed actinobacillus or actinobacillus-like species, [pasteure ...19911930571
[identification and typing of actinobacillus actinomycetemcomitans by restriction endonuclease analysis].the aim of the present study was to investigate the potential of restriction endonuclease analysis (rea) for the identifying and typing of actinobacillus actinomycetemcomitans (aa). bacterial chromosomal dna was extracted and digested with bamhi, hindiii, sal i and xho i respectively and separated by agarose gel electrophoresis. dna fragment patterns of aa and haemophilus aphrophilus differed strikingly from each ether. the patterns of aa fdc y4 and aa atcc 29523, belonging to two different sero ...19901965593
detection of mediastinitis after heart transplantation by gallium-67 scintigraphy.we report the findings of a patient with post-cardiac transplant mediastinitis detected by 67ga-citrate imaging. fever and leukocytosis were the first clinical signs suggesting infection. the usual diagnostic modalities, including ct and ultrasound, failed to identify the site of infection. a 67ga scan showed intense abnormal uptake behind the sternum. the site of uptake was shown by necropsy to be necrotic tissue involving cardiac sutures, pulmonary arteries, and the aorta due to infection with ...19912022995
haemophilus aphrophilus endophthalmitis associated with a filtering bleb. 19912025158
[determination of dna base composition and levels of dna-dna hybridization of actinobacillus actinomycetemcomitans].using the strains of actinobacillus actinomycetemcomitans (aa) and haemophilus aphrophilus (haph) in experiments, the mole percent quanine plus cytosine content (mol% g + c) and level of dna-dna hybridization (dna homology) were determined for the first time in oral microbiologic study in china. dna was extracted by marmur's method. the mol% g + c of dna was determined by thermal denaturation method (tm). dna-dna hybridization was carried out by de ley's optical procedure (initial renaturation r ...19912032476
[bacterial spondylitis caused by haemophilus aphrophilus].four weeks after a slight scratch a 43-year-old man noted a severe back pain. physical examination gave suspicion of a bacterial spondylitis. from the biopsy of the involved vertebral body haemophilus aphrophilus was isolated under aerobic growth conditions.19912060962
ciprofloxacin treatment of haemophilus paraphrophilus brain abscess. 19912071920
outer membrane proteins of actinobacillus actinomycetemcomitans and haemophilus aphrophilus studied by sds-page and immunoblotting.this investigation characterized and compared outer membrane proteins (omp) of the closely related actinobacillus actinomycetemcomitans and haemophilus aphrophilus by means of sds-page patterns and reactions on immunoblots with rabbit antiserum against a. actinomycetemcomitans fdc y4. reactions with serum from a patient with papillon lefévre syndrome (pls), from whom periodontal wild strains of a. actinomycetemcomitans had been isolated, were also studied. omp were purified with selective solubi ...19902080070
an in vitro method to study the adherence of bacteria to saliva-treated tooth enamel sections.an in vitro bacterial adherence assay which employed human tooth enamel sections precoated with saliva and an epifluorescent staining technique with acridine orange was developed. the assay was used to study the adherence properties of fresh and type strains of the following oral bacterial species: bacteroides gingivalis, bacteroides intermedius, capnocytophaga species, haemophilus aphrophilus, actinobacillus actinomycetemcomitans, peptostreptococcus species, veillonella species, actinomyces isr ...19902087341
[empyema caused by haemophilus aphrophilus with secondary leukemoid reaction]. 19902090900
sialidase (neuraminidase) activity among gram-negative anaerobic and capnophilic bacteria.a filter paper spot test with 2'-(4-methylumbelliferyl)-alpha-d-n-acetylneuraminic acid as a substrate was used to study the prevalence of sialidase activity among gram-negative anaerobic and capnophilic bacteria. a total of 567 isolates representing four genera of obligate anaerobes and four genera of capnophilic organisms was tested. sialidase activity was detected in 94% of 66 isolates from the bacteroides fragilis group, 98% of 66 b. bivius isolates, and all isolates of the following species ...19902108991
multivariate analysis of quantitative chemical and enzymic characterization data in classification of actinobacillus, haemophilus and pasteurella spp.chemotaxonomic data for strains of actinobacillus, haemophilus and pasteurella spp. were analysed using three multivariate statistical strategies: principal components, partial least squares discriminant, and soft independent modelling of class analogy. the species comprised actinobacillus actinomycetemcomitans. haemophilus aphrophilus, h. paraphrophilus, h. influenzae, pasteurella multocida, p. haemolytica and p. ureae. strains were characterized by cell sugar and fatty acid composition, lysis ...19902118166
monoclonal antibody-coated latex agglutination assay for identification of actinobacillus actinomycetemcomitans.three monoclonal antibodies (mabs) to lipopolysaccharide of actinobacillus actinomycetemcomitans strain y4 (serotype b) and eight mabs to a serotype b-specific polysaccharide antigen of strain y4 were obtained. latex particles sensitized with an mab to the y4 lipopolysaccharide produced a positive agglutination with whole cells of all three serotypes of a. actinomycetemcomitans, but not with haemophilus aphrophilus, haemophilus paraphrophilus, haemophilus influenzae, porphyromonas (bacteroides) ...19902124492
[isolation of haemophilus aphrophilus from a blood culture].the authors describe the method of isolation and identification and differential diagnosis from a morphologically, cultivation and biochemically close species h. (a.) actinomycemcomitans. they discuss the problem of the taxonomic classification of the two species.19902142621
preferential adsorption of human neutrophil myeloperoxidase isoform iii by oral bacteria.neutrophil myeloperoxidase (mpo) adsorbs to bacteria as a pre-requisite for killing by the mpo/hydrogen-peroxide/chloride system. three chromatographically distinct isoforms of mpo (mpo i, mpo ii, and mpo iii) have been isolated from human neutrophils. the purpose of this study was to determine whether oral bacteria--including actinobacillus actinomycetemcomitans, capnocytophaga sputigena, haemophilus aphrophilus, and eikenella corrodens--differentially adsorb the three major isoforms of mpo fro ...19902172341
comparison of the rim-h rapid identification kit with conventional tests for the identification of haemophilus spp.a commercially available system, the rim-h system (austin biological laboratories, austin, tex.), was evaluated for its ability to rapidly and accurately identify various haemophilus spp. a total of 110 clinical isolates were tested by both the rim and conventional identification procedures. the rim agreed with the standard identification for 100% of the haemophilus influenzae (76 of 76) and 92.0% of the haemophilus parainfluenzae (23 of 25) isolates tested. the identifications of haemophilus pa ...19902203817
haemophilus aphrophilus endocarditis.we report a case of haemophilus aphrophilus endocarditis involving mitral and tricupsid valves in a boy with congenital heart disease. he had received dental treatment without antibiotic cover. prolonged high-dose ampicillin with gentamicin was necessary for cure. short courses of ampicillin alone may not successfully treat h. aphrophilus.19902206842
inter- and intrafamilial similarities of rrna cistrons of the pasteurellaceae.we performed hybridizations between labeled rrnas from seven representative members of the family pasteurellaceae and from three other taxa on the one hand and dnas from 53 strains known or presumed to belong to the pasteurellaceae on the other hand. the members of the pasteurellaceae are most closely related to members of the enterobacteriaceae, the vibrionaceae, the aeromonadaceae, and the genus alteromonas. the family pasteurellaceae is very heterogeneous. there are at least seven rrna branch ...19902223605
identification of haemophilus aphrophilus and actinobacillus actinomycetemcomitans by dna-dna hybridization and genetic transformation.dna-dna hybridization was used to identify clinical isolates as haemophilus aphrophilus or actinobacillus actinomycetemcomitans. some of the isolates were naturally competent for genetic transformation and were also used as dna recipients for identification of other isolates. the results obtained by hybridization were supported by interstrain-to-intrastrain transformation ratios. distinction between the closely related species h. aphrophilus and a. actinomycetemcomitans was generally clear-cut b ...19902229383
haemophilus aphrophilus isolated from blood.h. aphrophilus was isolated from 11 successively cultivated blood samples of a male (72) with a fatal atypical endocarditis. identification and differential diagnosis from actinobacillus (haemophilus) actinomycetemcomitans was done on the basis of phenotypic characters of the microorganism.19902248686
differentiation between actinobacillus (haemophilus) actinomycetemcomitans, haemophilus aphrophilus and haemophilus paraphrophilus by multilocus enzyme electrophoresis.genetic relationships among isolates assigned to actinobacillus actinomycetemcomitans, haemophilus aphrophilus and h. paraphrophilus were determined by analysis of electrophoretically demonstrable allelic variation in 14 structural genes encoding metabolic enzymes. among the 51 isolates analysed there were 25 electrophoretic types (ets), among which mean genetic diversity per locus was 0.753. cluster analysis of ets demonstrated one well-defined group of 11 ets representing solely the genotypes ...19902269878
multivariate analyses of carbohydrate data from lipopolysaccharides of actinobacillus (haemophilus) actinomycetemcomitans, haemophilus aphrophilus, and haemophilus paraphrophilus.the taxonomic distinction between actinobacillus (haemophilus) actinomycetemcomitans and haemophilus aphrophilus and the taxonomic distinction between h. aphrophilus and haemophilus paraphrophilus have been questioned. this study was done to determine whether multivariate statistical analyses of carbohydrate data from lipopolysaccharides could be used to distinguish between these closely related species. lipopolysaccharides were extracted with phenol-water and purified. carbohydrates were assess ...19902275855
mast cell activation in sterile bladder and prostate inflammation.sterile inflammation of the bladder has often been associated with interstitial cystitis (ic), a urologic condition of unknown etiology, predominantly affecting young and middle-aged females, for which no effective therapy is known. recent reports have indicated that ic is associated with an increased number of bladder mast cells. here we report the case of a middle-aged man with chronic sterile hematuria, dysuria and lower abdominal pain associated with a high number of bladder and prostate mas ...19902276845
species-specific oligodeoxynucleotide probes for the identification of periodontal bacteria.oligodeoxynucleotide probes were developed for identification of the periodontal bacteria actinobacillus actinomycetemcomitans, bacteroides gingivalis, b. intermedius types i and ii, b. forsythus, eikenella corrodens, fusobacterium nucleatum, haemophilus aphrophilus, streptococcus intermedius, and wolinella recta. probes were designed by sequencing the 16s rrna for each bacterium, identifying hypervariable regions, and chemically synthesizing species-specific probes. these probes were specific w ...19902312676
film panel cases society of pediatric radiology. haemophilus aphrophilus. 19902349027
synthetic oligodeoxynucleotide probes for the rapid detection of bacteria associated with human periodontitis.analysis of the subgingival microflora has recently implicated actinobacillus (haemophilus) actinomycetemcomitans and several black bacteroides species in the aetiology of juvenile, adult and rapidly progressing periodontitis. rapid bacteriological diagnosis has been hampered by the slow growth and fastidious nature of these bacteria. to construct diagnostic probes, dideoxy sequencing of the 16s rrna molecules from a. (h.) actinomycetemcomitans, haemophilus aphrophilus, bacteroides gingivalis, b ...19882469776
ignorance about listeria. 19892504402
[aortic and mitral endocarditis caused by haemophilus paraphrophilus with abscess of the aortic ring and cerebral embolism].we report the first case of aortic and mitral haemophilus paraphrophilus endocarditis complicated by abscess of the aortic annulus in a 30-year old man with post-rheumatic mitral regurgitation. we recall the peculiar clinical features and course of this bacterial endocarditis of uncommon origin. we insist, in particular, on the occurrence of cerebral embolism and on the two-dimensional echocardiographic diagnosis of an aortic annulus abscess confirmed at surgery. cure was obtained by aortic and ...19892512879
in vivo chemotaxis evoked by actinobacillus actinomycetemcomitans and haemophilus aphrophilus.the chemotaxis-evoking capacity of 5 actinobacillus actinomycetemcomitans and 5 haemophilus aphrophilus strains were studied in a tissue cage model in rabbits. a significant increase of the total number of polymorphonuclear leukocytes was induced in the tissue cage fluid by both viable and killed bacteria, reaching a maximum after 12-24 h. in parallel, the proportion of viable/non viable leukocytes increased. the leukocyte counts declined during the following 24-48 h in all chambers except in th ...19892628865
[haemophilus aphrophilus epidural abscess studied by nuclear magnetic resonance]. 19892660661
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