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'meningitis by the score'. 1975664
proceedings: long-term therapy with antibiotics in chronic bronchitis.longterm therapy of chronic bacterial bronchitis assumes two forms: (a) therapy of acute exacerbations, and (b) continuous longterm prophylaxis, chiefly during the 4-7 winter months. longterm prophylaxis should be confined exclusively to patients with two or more severe annual exacerbations. the commonest pathogens, haemophilus influenzae and pneumococci, are usually sensitive to ampicillin and amoxycillin, cotrimoxazole (bactrim or eusaprim) and tetracyclines.19752971
the role of some microorganisms in the pathogenesis of non-specific bronchopulmonary infections. 19763770
bactericidal activity and pharmacology of flucloxacillin.flucloxacillin, a recent addition to the group of isoxazolyl penicillins, was studied in vitro and in normal volunteers. the bactericidal activity of the drug against most strains of gram-positive bacteria including penicillin-resistant staphylococcus aureus was similar to that of oxacillin and approximately fourfold greater than that of cloxacillin. each of the three penicillins was administered orally to a group of ten volunteers for eight days in a dose of 500 mg four times a day. the mean co ...19763969
in vitro susceptibility studies with josamycin and erythromycin.the in vitro activity of josamycin and erythromycin against five bacterial species was compared. in general, erythromycin was slightly more active by weight than josamycin, although both agents had a similar range of activity.19764012
gentamicin-susceptibility of various pathogens isolated from clinical materials.we studied on the antibacterial activity of gentamicin against various pathogens isolated from clinical materials mainly isolated during 1974 and 1975, comparing with other antibiotics. beta hemolytic streptococci, pneumococci and enterococci are less susceptible to gentamicin than staphylococci. staph, aureus and staph. epidermidis resistant to various antibiotics are very susceptible to gentamicin, and no resistant strain to this drug was found. haemophilus influenzae, h. parainfluenzae and h. ...19765619
bacterial meningitis in infancy and childhood in lusaka (one year prospective sturdy).in approximately 10,000 admissions in a 12 months period, at university teaching hospital, lusaka 85 cases of meningitis were recorded. the signs and symptoms in these patients do not greatly differ from other similar studies in africa. the commonest organism isolated was pneumococcus. there was high mortality rate which was to a large extent due to parents not bringing their children to hospital early enough for medical treatment to be instituted. this is borne out by the fact that 50% of the c ...19755834
antibacterial activity of eight cephalosporins against haemophilus influenzae and streptococcus pneumoniae. 19766415
oxygen-independent inactivation of haemophilus influenzae transforming dna by monochromatic radiation: action spectrum, effect of histitine and repair. 19766978
should fiberoptic bronchoscopy aspirates be cultured?the reliability of fiberoptic bronchoscopy as a method to study the bacteriology of the lower respiratory tract was tested. the procedure used was suction aspiration through the inner channel after topical anesthesia with lidocaine. to detect contamination by oropharyngeal bacteria, the aspirates were cultured in patients with no evidence of active infection, comparison was made with results of transtracheal aspiration cultures, and the aspirate was tested for the presence of an oral dye marker. ...19767173
immunoglobulins in maxillary sinus secretion.the immunoglobin pattern was studied in maxillary sinus secretion of 17 patients with maxillary sinusitis. by single radial immunodiffusion, measurable amounts of igm were found in the secretions of 15 patients, and of iga and igg in the secretion of all patients. in purulent sinus secretion, iga levels were significantly lower than in serous secretion. of the total content of iga in sinus secretion, 30% (purulent secretion) and 43% (serous secretion) were calculated to be locally produced, on a ...19767915
immune response to acute otitis media: association between middle ear fluid antibody and the clearing of clinical infection.clearing of the middle ear fluid in patients with acute otitis media due to streptococcus pneumoniae or haemophilus influenzae was significantly associated with the presence and concentration of specific antibody in the middle ear fluid at the time of diagnosis.19769424
bacteriology and antibiotics in acute suppurative otitis media.one hundred and forty-seven patients with acute suppurative otitis media, were divided into three groups and treated with antibiotics (azidocillin, ampicillin, and cephalexin). the therapeutic effect was assessed bacterioloically by swabbing from the aural discharge and from the nasopharynx on the first, second, third, and seventh day after initiation of treatment. in addition, the concentration of antibiotic in the aural discharge and in the nasopharynx was determined. as compared with other pu ...19769519
relation of concentrations of bacteria and bacterial antigen in cerebrospinal fluid to prognosis in patients with bacterial meningitis. 197713301
bacteriological examination of sputum (author's transl).the aa., emphasizing the importance of ascertain the bronchial source of microorganisms recoverable from sputum, recommend for this purpose the use of properly collected sputum specimens and the application of washing treatment for mucopurulent materials. the aim of the present study has been to verify the effectiveness of mechanical apparatus to homogenize sputum without affecting the viability of resident microorganisms and the usefulness of prior microscopical examination to establish the rat ...197613741
study on the viscolytic activity of the sputum (author's transl).several mucolytic agents were evaluated on sputum for testing their viscolytic activity and the bacterial tollerance to each of them. proteolytic enzymes (trypsin, pepsin, papain, pancreatin), kj, and dithiothreitol (or its derivatives) were better tollerated by common respiratory pathogens (h. influenzae, d. pneumoniae, klebsiella, etc.) than other mucolytic agents, as acetil-cysteine, cisteamine-hcl, tension active substances, mercaptoethanol, and others. the dithiothreitol showed also one of ...197613742
new selective beta2-adrenoreceptor stimulants 5(1-hydroxy-2-isopropylaminobutyl)- and 5-(1-hydroxy-2-ethylaminobutyl)-8-hydroxycarbostyril hydrochlorides (opc-2009 and opc-2030) and cyclic amp concentration. 197715090
5-methoxytryptamine: stimulation of 5-ht receptors mediating the rat hyperactivity syndrome and blood platelet aggregation. 197615408
purification and properties of the methane mono-oxygenase enzyme system from methylosinus trichosporium ob3b.1. a three-component enzyme system that catalyses the oxidation of methane to methanol has been highly purified from methylosinus trichosporium. 2. the components are (i) a soluble co-binding cytochrome c, (ii) a copper-containing protein and (iii) a small protein; the mol. wts. are 13 000, 47 000 and 9400 respectively. the cytochrome component cannot be replaced by similar cytochrome purified from pseudomonas extorquens or by horse heart cytochrome c. 3. the stoicheiometry suggests a mono-oxyge ...197715544
prominent role of lysosomes in the proteolysis of rat liver mitochondria at neutral ph. 197715866
hormonal control of zinc uptake and binding in the rat dorsolateral prostate.the zinc uptake in the dorsolateral prostate of rats was studied after different hormonal manipulations. orchiectomy reduced the uptake of 65zn. administration of estradiol benzoate to orchiectomized rats doubled the 65zn uptake, a phenomenon which was not observed in orchiectomized-adrenalectomized rats. adrenalectomy in orchiectomized rats had no effect on the concentration of radioactivity beyond the castration-induced decrease. a prolactin release inhibitor, 6-methyl-8-erogelenylacetamide, r ...197715955
mycotoxicoses. 197615366
quantitative relationship between neurons of a different mediator nature in the motor nuclei of the cat spinal cord cervical thickening.a histochemical analysis of the neuronal composition of the motor nuclei was performed on the cat cervical spinal cord. motoneurons were distinguished by the location of ache in the perinuclear space, on the membranes of the granular reticulum, on the axolemma, neurofilaments and neurotubuli, in the synaptosomes and the synaptic cleft; this evidences for the perinuclear synthesis of this enzyme and its transport within the axoplasm. their amount reached 40-65% of the whole neuronal population. a ...197716229
the transport of l-leucine into the brain of the rat in vivo: saturable and non-saturable components of influx. 197716270
gas levels and biochemical changes in cerebrospinal fluid after experimental brain injury: its effect on cerebral circulation and cerebral metabolism as well as its management.1. after brain damage experimentally induced by balloon compression, liquor of the cistern shows a metabolic acidosis with significantly increased lactate level and l/p quotient, and statistically distinct decrease of ph, oxygen tension, and bicarbonate level. 2. there is a considerable disproportion between the changes of acid-base metabolism, gas tension, and metabolic products in cerebrospinal fluid compared with much less expressed disturbances in the blood. 3. the advanced edematous phase s ...197615353
light-activated calcium release from sonicated bovine retinal rod outer segment disks.calcium trapped within sonicated and resealed bovine rod outer segment disks is released upon light exposure with a stoichiometry of 0.75 +/- 0.05 calcium for each rhodopsin bleached. the amount of calcium liberated is proportional to the amount of bleaching in the range of 20 to 100% bleaching and is relatively insensitive to the internal trapped calcium concentration. the results are obtained using a flow system in which the disk membrane vesicles are adsorbed on glass particle supported by a ...197714680
bacterial meningitis in the adult. 197712560
interactions between viruses and bacteria in patients with chronic bronchitis.the possibility that viral infections of the respiratory tract might predispose to bacterial colonization or infection was studied in 120 patients with chronic obstructive pulmonary disease and 30 control subjects; these individuals were observed for seven years. the ratio of the number of observed to the number of expected associations between viruses and bacteria was 2.43 (p = 0.037) for the pair influenza virus and streptococcus pneumoniae and was 2.06 (p = 0.056) for influenza virus and haem ...197612235
a case of hemophilus influenzae meningitis with proven resistance to ampicillin. case report. 197611171
the detection of soluble bacterial antigens studied in various pathological substances using counterimmunoelectrophoresis. contribution to diagnosis(151 cases).examinations for soluble bacterial antigens using counter-immunoelectrophoresis (c.i.e.) was carried out in 151 patients suspected of suffering from various infectious syndrome were successful for s. pneumoniae, h. influenzae b, n. meningitis, sero-group b and d streptococcus. thus meningitis and pneumonia represent those areas in which the technique is particularly useful. apart from its rapidity--result in a hour--c.i.e., in association with bacteriology, makes possible an increase in aetiolog ...197717848
the speed of passive dendritic conduction of synaptic potentials in a model montoneurone. 197717867
bacterial precipitins and their immunoglobulin class in atopic asthma, non-atopic asthma, and chronic bronchitis.in a study of groups of patients with atopic (extrinsic) asthma, non-atopic (intrinsic) asthma, and chronic bronchitis, no difference could be detected in the numbers having precipitating antibodies against species specific antigens from staphylococcus aureus or streptococcus pneumoniae compared to suitably matched control subjects. precipitating antibodies against species specific antigens from haemophilus influenzae, demonstrated in this investigation by double diffusion in agar gel, were foun ...19769702
identification of essential histidine residues of aminoacylase by photooxidation and by reaction with diethylpyrocarbonate.state and function of the histidine residues of aminoacylase were investigated by photoxidation in the presence of methylene blue and by chemical modification with diethylpyrocarbonate. complete inactivation of the enzyme was observed after oxidation of 4 histidine residues. from the ph dependence of the photooxidation it becomes evident that the inactivation of the enzyme is not a consequence of the simultaneous oxidation of tryptophan residues. the enzyme is also inactivated by chemical modifi ...197717959
letter: diagnostic value of the detection of specific polysaccharides by counterelectrophoresis in meningitis in children. 50 cases. 19766946
bacterial flora of the respiratory tract in chronic bronchitis: comparison of transtracheal, fiberbronchoscopic, and oropharyngeal sampling methods. 197718077
the cellulase enzyme system during growth and development of acanthamoeba castellanii (author's transl).it could be shown that extracts of growing cultures of acanthamoeba castellanii contained a cellulose degrading system. reducing sugars are split off by one component of this system at an optimum of ph 4, another enzyme changes the viscosity at an optimum of ph 6, and a third component is a beta-glucosidase with an optimum at ph 3.5. at ph 4 the cellulose degradation products are cellobiose and glucose; at ph 6 higher molecular weight oligosaccharides are produced. during the development from tr ...197718125
a survey of isolates of streptococcus pneumoniae.over eight months 130 isolates of streptococcus pneumoniae were examined for sensitivity to penicillin, tetracycline, cephalosporin and co-trimoxozole. except in one doubtful case all isolates were sensitive to penicillin. all were sensitive to cephalosporin. 6.9 percent is isolates were resistant to co-trimoxozole. it is suggested that penicillin remains the drug of choice in the treatment of pneumococcal infections.19766927
bacteriological examinations of bronchial secretion in children with non-specific bronchopulmonary disease (author's transl).one thousand specimens of bronchial secretion from children with non-specific respiratory diseases have been examined bacteriologically. in spite of the complex nature of acute and especially of chronic respiratory disease the role of bacterial infection should not be underestimated. thirty per cent of the specimens were sterile. more than 20 per cent of the bacterial species isolated from bronchial secretion were pathogenic. relatively frequent was the isolation of e. coli and of pathogenic sta ...19755823
susceptibility of pneumococci and haemophilus influenzae to antibacterial agents.strains of diplococcus pneumoniae and haemophilus influenzae were tested for susceptibility to numerous antibiotics by a twofold agar dilution method using an inocula replicator. undiluted, fully grown broth cultures were used as inocula for both species, and cultures of pneumococci diluted 1:1,000 were also tested. the antibiotics included most of those in common use in the united states as well as some chemical modifications recently approved and others that are under investigation. the most s ...19765052
microorganisms in chronic otitis media with effusion.a total of 175 effusions obtained from 172 patients suffering from chronic otitis media with effusions was examined for bacterial smear and culture. eighty percent showed positive bacterial smear, but only 49% yielded positive bacterial culture. the mucoid effusions had positive cultures in only 37%, whereas the bacterial culture rate was higher in serous (59%) and leukocytic (64%) types. the isolation of common pathogens accounted for about 50% of the isolates, and nonpathogens accounted for th ...19765041
otitis media in the newborn infant.otitis media occurs frequently in newborn infants and often is associated with systemic infection. patients studied at the boston city hospital have provided preliminary data on the otoscopic findings, tympanometric patterns and etiologic agents characteristic of otitis in this age group.19765040
immunology and microbiology in acute otitis media.various immunological parameters were measured in serum, middle ear fluid (mef), and lymphocytes from peripheral blood and mef of infants with acute otitis media due to s. pneumoniae or h. influenzae. approximately half of 131 patients had ige specific antibody to the infecting bacterium as determined by the indirect fluorescent antibody (ifa) technique. seventy-one percent of these ige positive patients had ige specific antibody in the mef. total ige concentration was found to be from an averag ...19765039
prognosis of pneumonia: sputum culture and gram stain. 197718694
the influence of adrenergic blocking agents on the pressor response to vasopressin. 197718878
evaluation of the antibiotic effect of treatment of maxillary sinusitis.as the effect of antibiotic treatment of maxillary sinusitis has been questioned, the elimination of bacteria from sinus secretions was studied during antibiotic treatment. penicillin v, azidocillin, tetracycline or doxycycline was administered to 54 patients with maxillary sinusitis. samples of sinus secretion were aspirated both before treatment and 2-3 days after the onset of treatment. when the antibiotic concentration was below the upper limit of mic for sensitivity group 1, bacterial growt ...19751848
influence of age and sex on the acute toxicity of two steroid anesthetics in rats.during the newborn period, there is a hight increase of the acute toxicity of two steroid anaesthetics (hydroxydione, althesin) given by intraperitoneal route on the rat as also an influence of hormonal conditions.197719132
the influence of bacterial superinfection on the clinical course of influenza. studies from the influenza epidemics in stockholm during the winters 1969-70 and 1971-72.during two epidemics of influenza a infection in stockholm 1969-72, 249 cases were selected for a study on the effect of bacterial superinfection. bacterial involvement was demonstrated through cultures and serologic reactions. the occurrence of c-reactive protein in increased amount in serum was significantly more common in the group which had the strongest indication of bacterial infection. an increased duration of fever, and a higher incidence of pneumonia, leukocytosis and erythrocyte sedime ...19751847
editorial: bronchiectasis today. 19751138
bacterial recombination.the most perspective trends in studying the mechanisms of genetic exchanges in bacteria are discussed. they are the following. 1. comparison of the recombination stages in various biological phenomena such as transformation, transduction (generalized), conjugation (common and single-stranded) and transfection. 2. characterization of genetic determinants and basic enzymes of the recombination process in rec-systems of different bacteria. 3. determination of some peculiar features of the intrageni ...197719345
acute otitis media. a clinical bacteriological and serological study of children with frequent episodes of acute otitis media.a series of episodes of acute otitis media was studied with reference to bacterial findings and specific serological responses in 48 children with histories of frequent episodes before. d. pneumoniae and h. influenzae were the most frequently isolated pathogens. re-isolations after therapy were often made in episodes with slow healing or therapeutic failure. most children harboured pathogens in nasopharynx even when they had no signs of respiratory tract infections. homologous relapses were seen ...1975885
soterenol and related compounds: molecular modifications and changes in selectivity, affinity and efficacy at beta-adrenoreceptors in guinea-pig atria and trachea. 197720258
effect of contrast media on the rate of oxidation of nadp.h and nad.h by rat liver microsomes.the effect of roentgen-contrast media on the activity of the nadp'n and nad'n-dependent electron-transport chains of the rats liver microsomes was studied. bilignost, cardiotrast, triiotrast and triombrin are shown to lower the rate of the nadp'n oxidation by the rats' liver microsomes and have no effect (except for triiotrast) upon the rate of the nad'n oxidation. it is presumed that the relative resistance of the nad'n-specific flavoproteid is due to the presence of a hydrophobic layer impervi ...197720329
comparative study of virological infections in asthmatic and nonasthmatic children.the author shows complex analyses: clinical, laboratory, x-rays, bronchoscopical, bronchographical and measuring lung function tests as well as the serological examinations in blood serum of both groups of asthmatic and nonasthmatic children with virological infection. the calculation of statistically significant differences between the various diagnostical results of both groups has confirmed that in asthmatic children virological infection of the respiratory tract, pathological findings in x-r ...1975884
current status of treatment of pneumonia.proper treatment of pneumonia is dependent upon a correct diagnosis. pneumonia may be due to infectious agents, allergic phenomena, or chemical causes. treatment regimens are outlined for the various types of pneumonia--pneumococcal, staphylococcal, fungal, and pneumonia due to gram-negative and anaerobic gram-negative bacilli, to blastomyces dermatitidis, and to the parasite pneumocystis carinii. in discussing current concepts of treatment, several well-known methods are emphasized, as well as ...1975792
bacteriologic diagnosis of acute pneumonia. comparison of sputum, transtracheal aspirates, and lung aspirates. 1976521
the reversible inhibition of gluconeogenesis in kidney cortex by diazenedicarboxylic acid bis (n,n-dimethylamide). 197721091
regulation of adenosine cyclic 3',5'-monophosphate and guanosine cyclic 3',5'-monophosphate levels and contractility in bovine tracheal smooth muscle. 197716208
properties of deoxynucleoside 5'-monophosphatase induced by bacteriophage t5 after infection of escherichia coli.bacteriophage t5 induced a deoxynucleoside 5'-monophosphatase during its infection of escherichia coli. the enzyme was purified about 100-fold. it was clearly distinct from the host 5'-nucleotidase activity in its physical characteristics and substrate specificity. the enzyme was active on deoxynucleoside 5'-monophosphates but was not active as a phosphatase on ribonucleotides, deoxynucleoside 5'-triphosphates, deoxynucleoside 3'-monophosphates, or deoxyoligonucleotides. furthermore, it did not ...197721305
prune-belly syndrome in a 54-year-old man.a 54-year-old man was diagnosed as having prune-belly syndrome, a deficiency of abdominal musculature, cryptorchidism, abnormalities of the urinary tract, and chronic renal failure. the patient was asymptomatic until the diagnosis was made at the age of 54 years; he is one of the oldest living patients to be reported in detail. recognition of the syndrome in adults with chronic renal failure is important.197716145
effect of pindolol on experimental hypertension in the dog. comparison with propranolol, practolol, oxprenolol, bupranolol, alprenolol and butoxamine. 197721636
acute bacteremia in asplenic renal transplant patients.four cases of overwhelming bacteremia were observed in asplenic renal transplant patients 12 to 20 months after transplantation. the bacteriologic findings and presentation of these infections are characteristic of post-splenectomy sepsis reported in nontransplant patients. it is suggested that the absence of the spleen, more than immunosuppression with azathioprine and prednisone, predisposes to these late, uncommon infections after transplantation.197716144
liver enzyme adaptation after lithium administration in handled and nonhandled rats.we have examined the interaction of lithium administration and the infant stimulation procedure of handling on hormonally regulated enzymes of liver. animals handled in infancy show an increased morning corticosterone level in response to lithium feeding and markedly elevated serum glucose during refeeding following a two day fast, when compared to non-handled control animals. lithium alters serum corticosterone both in response to the stress of fasting, and during the diurnal cycle following gl ...197721840
isolation and characterization of sheep pepsin.sheep pepsin was isolated (approx. 120-fold purification) from aqueous abomasal homogenates by (1) ph fractionation, (2) chromatography on sepharose 4b-poly-l-lysine columns and (3) gel filtration on sephadex g-100. the enzyme had mol.wt. approx. 34000, n-terminal valine and c-terminal alanine. the amino acid composition of sheep pepsin was generally similar to that of pig and ox pepsins, with a very low content of basic residues and a high content of acidic and hydroxy-amino acids. the ph optim ...197715546
physiology of competence in bacteria. 197716417
effect of imidazoles and ph on aging of phosphylated acetylcholinesterase. 197716611
amine binding sites in acyl intermediates of transglutaminases. human blood plasma enzyme (activated coagulation factor xiii) and guinea pig liver enzyme. 197716916
effect of h+ on spontaneous neuronal activity in the surface layer of the rat medulla oblongata in vitro.the effect of changing extracellular ph (phe) on the spontaneous activity of neurons in brain slices taken from the ventral layer of the rat medulla oblongata was compared to the response of neurons in dorsal slices. in the ventral medulla, more than 50% of the neurons were excited by h+. these neurons were found just lateral to the pyramidal tract between the root of the hypoglossal nerve and the trapezoid body. in the dorsal medulla, low phe caused an inhibition of activity in most neurons, al ...197722840
non-specific pharmacodynamic action of a piperidyl derivative (thioridazinum) of phenothiazine on the reactivity of the genital tract. 197722884
purification and characterization of sphingomyelinase from human brain. 197716918
compartmentalization of adenosine 3':5'-monophosphate and adenosine 3':5'-monophosphate-dependent protein kinase in heart tissue.in rabbit heart homogenates about 50% of the camp-dependent protein kinase activity was associated with the low speed particulate fraction. in homogenates of rat or beef heart this fraction represented approximately 30% of the activity. the percentage of the enzyme in the particulate fraction was not appreciably affected either by preparing more dilute homogenates or by aging homogenates for up to 2 h before centrifugation. the particulate enzyme was not solubilized at physiological ionic streng ...197716921
the use of counter-immuno-electrophoresis to identify causative organisms in bacterial meningitis: experience in cape town.crebrospinal fluid (csf) from 142 patients was tested for the presence of haemophilus influenzae, pneumococcal and meningococcal antigens by counter-immuno-electrophoresis with commercial antisera. group- or type-specific antigen was detected in the csf of 67% of 64 patients with meningitis proved by culture to be due to these organisms, and in 10 of 25 patients with purulent meningitis but negative cultures. no false positive results were obtained in 24 normal csf specimens, or in the csf of 29 ...197717927
bacteremia in hospitalized children.the results of 6,951 blood cultures taken from hospitalized children are reviewed. one or more organisms grew from 6% (399) of the cultures, of which 189 (two thirds) were considered to represent confirmed bacteremia. the most common organisms associated with bacteremia were haemophilus influenzae, streptococcus pneumoniae, enterobacteriaceae, and staphylococcus aureus. patients with deficient host defenses (newborns, oncology patients) with bacteremia had a higher mortality than normal children ...197718005
effect of 30 months in a locked environment on the microbial flora of dogs. 197718131
purification and properties of the nad(p)h:nitrate reductase of the yeast rhodotorula glutinis.the assimilatory nitrate reductase from the yeast rhodotorula glutinus has been purified 740-fold, its different catalytic activities have been characterized and some inhibitors studied. the purified enzyme (150 units per mg protein) contains a cytochrome of the b-557 type. an s20,w of 7.9 s was found by the use of sucrose density gradient centrifugation, and a stokes radius of 7.05 nm was determined by gel filtration. from these values, a molecular weight of 230 000 was estimated for the native ...197718182
activities of leishmania from human skin ulcers in small laboratory animals. 197723653
an adenosine 3':5'-monophosphate-adenosine binding protein from mouse liver. factors affecting the activation of the binding protein by adenosine 5'-triphosphate. 197823726
in vitro testosterone synthesis by testicular tissue of old mouse: the metabolism of 3beta-hydroxy-5-ene steroids. 197718359
sound spectrographic cry analysis of infants with bacterial meningitis. 197718378
partial purification of 6-(d-erythro-1',2',3'-trihydroxypropyl)-7,8-dihydropterin triphosphate synthetase from chicken liver.an enzyme that catalyzes the formation of 6-(d-erythro-1',2',3'-trihydroxypropyl)-7,8-dihydropterin triphosphate (d-erythrodihydroneopterin triphosphate) and formic acid from gtp has been purified about 3700-fold from homogenates of chicken liver. the molecular weight of the enzyme, d-erythrodihydroneopterin triphosphate synthetase (gtp cyclohydrolase), has been estimated to be 125,000 by gel filtration on ultrogel aca-34. the enzyme functions optimally between ph 8.0 and 9.2 and is considerably ...197718471
neurogenic vasodilation of cat cerebral arteries.transmural nerve stimulation (tns) with 0.3-msec pulses between 1 and 25 hz dilated cat cerebral artery segments in the presence of active muscle tone. maximum vasodilatation occurred at 8 hz. the dilator response to exogenous acetylcholine, but not to tns, was abolished by atropine. neither physostigmine nor hemicholinium affected the dilator response to tns, which persisted after administration of guanethidine, phenoxybenzamine, propranolol, reserpine, and chronic sympathectomy. however, it wa ...197824505
acute purulent otitis media in children older than 5 years. incidence of haemophilus as a causative organism.it is generally believed that haemophilus influenzae is not commonly a causative agent of otitis media in children older than 5 years of age. we recently studied cases of 58 children, aged from 5 to 9 years, who had acute otitis media. haemophilus species were the causative agents in 36% of cases. this high incidence of haemophilus isolation from the middle ear exudate of these children has important therapeutic implications; antibiotics effective against h influenzae should be employed when dea ...197718616
nuclease detection in sds-polyacrylamide gel electrophoresis. 197718945
pd2-, pa2- and pd2'-values of a series of compounds in a histaminic and a cholinergic system.(1) affinity and intrinsic activity values of 75 compounds for a histaminergic and a cholinergic system are presented. the quantitative correlations between the affinity values of 35 compounds and some physicochemical constants (van der waals volume, lipophilicity, number of hydrogen atoms on the protonated amine) are discussed. (2) absence of systematic differences between pd2 and pa2 of partial agonists supports the assumption that these values are equivalent expressions of the same affinity. ...197719265
the influence of ph, low sodium ion concentration and methotrexate on the jejunal-surface ph: a model for folic acid transfer. 197719312
the microbiology of chronic middle ear effusions in children.serous otitis media is the most common cause of hearing loss in childhood. traditionally, this entity has been felt to be a sterile process. this study was undertaken to determine the incidence of infected middle ear fluid in patients with asymptomatic middle ear effusions. the external auditory canal, nasopharynx and middle ear fluids of 57 patients undergoing myringotomy were cultured. of 57 patients, 26 had positive cultures; 67% of patients less than 36 months of age had positive culture, wi ...197719659
acute bacterial meningitis at boston city hospital during 12 selected years, 1935-1972.data are presented on the occurrence of and mortality rate from acute bacterial meningitis at boston city hospital during 12 years between 1935 and 1972 selected in relation to the introduction of potent antibacterial agents. the most frequent causative organisms were streptococcus pneumoniae, neisseria meningitidis, and haemophilus influenzae, but large proportions were caused by other gram-positive cocci and gram-negative bacilli. the greatest reduction in mortality rate after antibiotics beca ...197720479
a new spectrophotometric assay for protein in cell extracts. 197720815
early interactions of competent bacteria with nucleic acids (author's transl). 197721387
effect of amisyl and spasmolytin on the blood supply and oxygen metabolism of the brain.the effect of benactyzine and spasmolytin adiphenine on the blood circulation in the oxygen consumption by the brain were investigated in experiments on dogs. the resulting data point to a biphasic action of benacyzine and spasmolytin on the blood supply of the brain (after a short-lived increase of the blood flow a clearcut decline of it is noted). the oxygen intake from the blood varies irregularly. in chlorophos-poisoned dogs the oxygen absorption by the brain changes under the effect of bena ...197826597
bacterial etiology of otitis media during the first six weeks of life.tympanocentesis was performed on 70 infants who had otitis media during the first six weeks of life. the bacteria isolated from their middle-ear effusions were streptococcus pneumoniae (13 patients), neisseria catarrhalis (11 patients), hemophilus influenzae (ten patients), enterobacteriaceae (four patients), staphylococcus aureus (four patients), streptococci (groups a and b) (three patients), and pseudomonas aeruginosa (two patients). thirty patients (42.9%) had middle-ear effusions which did ...197826735
interaction of folic acid with ribonuclease a. 197721693
comparison of the ribonucleoproteins of different rabies virus serotypes by radioimmunoassay.radioimmunoassay (ria) provides a sensitive serological procedure for detecting rabies virus ribonucleoprotein (rnp) as well as its specific antibodies, ria was carried out using highly purified rnps labelled by the chloramine-t method. this paper describes optimal conditions for iodination of rnp with high specific activity. the optimal concentrations of 125i, rnp, chloramine-t, and reducing agent as well as the effect of ph on the reaction were investigated. ria proved to be extremely sensitiv ...197722569
aerobic and anaerobic bacteriology of acute otitis media in children.tympanocentesis and aerobic and anaerobic cultivation of the middle-ear fluid obtained was performed through one or both tympanic membranes of 62 children with acute otitis media. aerobic bacteria alone, predominantely pneumococcus and hemophilus influenzae, were isolated from 57% of patients; anaerobic organisms alone, most commonly propionibacterium acnes and peptococcus, from 15%. thirteen percent yielded mixtures of aerobes and anaerobes, and several had multiple aerobic or anaerobic agents. ...197822590
neurochemical anatomy of the neuroendocrine hypothalamus. neurochemical anatomy of the hypothalamus.microdissection techniques for isolated removal of the various regions of the hypothalamus as well as the individual hypothalamic nuclei are detailed. recent development of biochemical microassays have made it possible that the concentrations of neurohormones, neuropeptides, neurotransmitters and their related enzymes could be detected in such a small volume of brain tissue than the hypothalamic nuclei. data available of the hypothalamic distribution of above substances are summarized. the possi ...197827266
central antagonism of tyramine-induced systemic hypotension by mescaline. 197722607
red cell oxygen affinity in fetal sheep: role of 2,3-dpg and adult hemoglobin.studies were carried out during fetal life in sheep to determine the relationship of 2,3-diphosphoglycerate (dpg), the intracellular red cell and extracellular ph, and the switchover to adult hemoglobin synthesis in regulating the position of the fetal red cell oxygen-affinity curve in utero. adult hemoglobin first appeared near 120 days of gestation. the mean oxygen tension at which hemoglobin is half saturated (p50) prior to 120 days of gestation remained constant at 13.9 +/- 0.3 (sd) torr and ...197827486
the increasing incidence of ampicillin-resistant haemophilus influenzae. a cause of otitis media.middle ear exudate was obtained by myringotomy or aspiration from 625 suburban children, aged 1 month to 12 years, who had acute otitis media; bacterial pathogens were isolated from 71%. haemophilus species were isolated from 212 (29%), and streptococcus pneumoniae from 205 (28%). from 1975 to august 1977, the number of haemophilus isolates found to be resistant to ampicillin has appreciably increased. currently, 8% of all cases of acute otitis media in the washington, dc, area are due to h infl ...197822767
the leeuwenhoek lecture, 1977. mouse teratocarcinoma and mouse embryo. 197827802
an in vivo and in vitro analysis of the alloantigen response in syrian hamsters. 197722946
experimental liver failure. a comparison between hepatectomy and hepatic devascularization in the pig.difficulties in the treatment of acute hepatic failure due to insufficient knowledge of the pathogenesis and the impredectability of the course has made an experimental model desirable. a number of such models have been proposed, among these hepatectomy and total hepatic devascularization. in the present study hepatectomy and total hepatic devascularization have been compared, as it has been doubted if hepatectomy, not including necrotic liver tissue, is a valid model of human hepatic failure. n ...197722974
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