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acute interstitial pneumonia [proceedings]. 1977193807
porcine abortion caused by actinobacillus equuli.actinobacillus equuli was isolated from an aborted porcine fetus. fetal lesions of peracute embolic septicemia were similar to those seen in septicemic actinobacillosis of older swine and other species. this case represented an individual rather than a herd abortion problem.1976965291
the isolation of actinobacillus equuli from equine abortion. 1976988818
removal of the reca c-terminus results in a conformational change in the reca-dna filament.the escherichia coli reca protein catalyzes homologous recombination of dna molecules, and the active form of the protein is a helical polymer that it forms around dna. previous image analysis of electron micrographs has revealed the reca protein to be organized into two domains or lobes within the reca-dna filament. we have now been able to show that a small modification of the reca protein by proteolysis results in a significant shift in the internal mass in the reca filament. we have cleaved ...19911804279
very early aortic responses during atherosclerosis induction in rabbits: measurement by duplex ultrasound. i. non-invasive study of aortic hyperresponsiveness to serotonin.we report the use of a non-invasive ultrasound method to visualize and measure changes in serotonin reactivity of the abdominal aorta during the early stages of atherosclerosis development. studies were performed at 0, 4, 8, and 14 weeks in new zealand white rabbits fed a diet enriched with 0.5% cholesterol. change in systolic vessel diameter at each examination was compared with changes in rabbits fed a control diet or rabbits on a diet enriched with cholesterol plus a concentrated marine lipid ...19902202316
an enzyme-linked immunosorbent assay (elisa) for the detection of antibodies to pasteurella pneumotropica in murine colonies.an elisa for the detection of class specific igg antibodies to pasteurella pneumotropica was developed for the serological diagnosis of infections in mouse colonies. heat inactivated whole cell preparations of an isolate of p. pneumotropica biotype heyl (strain p 166) served as antigen for the elisa procedure and for immune serum production in germ-free han:nmri mice. cross reactions with the autochthonous flora of han:nmri spf-mice were not observed, but were evident when a p. pneumotropica ant ...19882966877
septic periorchitis in a horse.a 2-month-old standard-bred colt with signs of abdominal pain and large scrotum was found to have septic periorchitis involving the right testis. surgical exploration of the abdomen and scrotum was performed; the colt was then castrated. actinobacillus equuli was isolated from specimens obtained at surgery. the colt was treated with broad-spectrum antibiotics and flunixin meglumine after surgery, and fully recovered. the clinical signs of periorchitis in the colt were similar to an inguinal/scro ...19883356574
effect of thyroid hormone therapy on plasma insulin-like growth factor i levels in normal subjects, hypothyroid patients and endemic cretins.the effect of thyroid hormone therapy (l-t4 or l-t3) on plasma immunoreactive insulin-like growth factor i (somatomedin c, sm-c) concentrations was studied in 8 normal controls, 14 primary hypothyroid subjects and in 7 patients with endemic cretinism. in normals basal levels of sm-c (1.56 +/- 0.77 u/ml) increased to (2.46 +/- 1.0 u/ml; l-t4) and to (2.9 +/- 0.95 u/ml; l-t3). plasma sm-c basal levels were significantly lower in primary hypothyroid subjects (0.81 +/- 0.48 u/ml) and increased to 2. ...19873570152
antigenic specificity of convalescent serum from cattle with haemophilus somnus-induced experimental abortion.the antigens of haemophilus somnus recognized by convalescent bovine serum were studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western blotting with a protein a-peroxidase conjugate. the same two 76k and 40k antigens were predominant in whole-bacterium preparations and in outer-membrane-enriched, triton x-100-insoluble fractions. the surface location of these two antigens was confirmed by absorbing antiserum with whole, live bacteria. absorption with h. somnus removed a ...19873570470
bacteria in enteric lesions of horses.thirty-three species of bacteria were isolated from the gastrointestinal mucosa of 23 adult horses and two foals. the bacteria isolated could be related to gross and microscopical lesions in some cases. clostridium perfringens type a, actinobacillus equuli, salmonella typhimurium and campylobacter coli biotype 1 could all be associated with gastrointestinal lesions. c jejuni biotype 1 and aeromonas hydrophila were both recovered in this study and have been identified as causes of enteritis in ho ...19863716108
qualitative and quantitative determination of enterobacterial common antigen (eca) with monoclonal antibodies: expression of eca by two actinobacillus species.the presence and quantity of the enterobacterial common antigen (eca) in several species belonging to the family enterobacteriaceae as well as to other gram-negative families were determined by a solid-phase enzyme-linked immunosorbent assay system and western blotting by using mouse monoclonal antibodies specific for eca. except for erwinia chrysanthemi, previously known to be an exception, all species known or presumed to belong to enterobacteriaceae produced eca (89 of 90 species). most speci ...19873818929
dipeptidyl carboxypeptidase from coryne bacterium equi. 19714332216
actinobacillus equuli infection in a litter of pigs and a review of previous reports on similar infections. 19734692514
actinobacillus equuli infection in pigs. 19734721944
[pharmacological studies of a lipase, ge-56, produced by pseudomonas sp. v. teratogenic effect of ga-56]. 19744858045
rapid chemical determination of chloramphenicol acetylation by cell extracts and by cell suspensions of e. coli b. 19684873865
an outbreak of neonatal deaths in foals due to actinobacillus equuli. 19725073135
a haemolytic variant of actinobacillus equuli causing an acute septicaemia in a foal. 19715290202
endocarditis in the pig caused by actinobacillus equuli: a field and an experimental case. 19715541940
increased frequency of isolation of pasteurella and actinobacillus species and related organisms.ninety-six clinical isolates of pasteurella, actinobacillus and related organisms were submitted to our reference laboratory for identification. the procedures for detecting the 11 identified species, pasteurella multocida, pasteurella haemolytica, pasteurella ureae, pasteurella pneumotropica, actinobacillus lignieresii, actinobacillus equuli, actinobacillus actinomycetemcomitans, haemophilus aphrophilus, cardiobacterium hominis, and two unnamed species of cdc groups, hb-5 and ef-4, are describe ...19846468368
prevalence of organisms described as actinobacillus suis or haemolytic actinobacillus equuli in the oral cavity of horses. comparative investigations of strains obtained and porcine strains of a. suis sensu stricto.evidence was obtained to indicate that equine strains of organisms previously described as actinobacillus suis or hemolytic variants of actinobacillus equuli might constitute a separate group of organisms provisionally designated taxon 11. four biovars were noticed within taxon 11. selected dna:dna hybridizations support the classification of the mannitol positive biovar 2 of taxon 11 distinct from porcine a. suis. the final taxonomical position of taxon 11, however, has to await more detailed g ...19846532108
study of agglutinins to brucella abortus, b canis and actinobacillus equuli in horses.horses at a veterinary teaching hospital and a slaughterhouse were surveyed for antibodies to brucella abortus, b canis and actinobacillus equuli. four of the 141 hospitalised horses and none of the 73 slaughtered horses had titres of 1:100 or greater to b abortus. six horses of both populations reacted to the card test. one was culture positive. a card test using b canis antigen was positive in 38 per cent of the sera from hospitalised horses and all of the slaughtered horses. twenty (27.4 per ...19826816581
influence of bacterial products on the motility of stallion spermatozoa.sterile filtrates were prepared from equine isolates of klebsiella pneumoniae (genitalium), proteus mirabilis, pseudomonas aeruginosa, escherichia coli, streptococcus zooepidemicus, streptococcus equisimilis, actinobacillus equuli, and corynebacterium equi and mixed individually with extended stallion semen. when diluted in the extended semen the filtrates represented bacterial populations of 0.5 x 10(6), 1 x 10(6), 2 x 10(6) and 4 x 10(6) cells/ml. ph values were recorded for each filtrate. spe ...19826962871
fibrinous pericarditis in the horse.during a period of 18 months, between july 1978 and january 1980, 4 adult horses were referred to the new york state college of veterinary medicine with evidence of congestive heart failure. characteristic clinical abnormalities included marked muffling of heart sounds, tachycardia, jugular vein distention, and peripheral edema. treatment with antibiotics, diuretics, and anti-inflammatory drugs was unsuccessful, and all four died or were euthanatized and necropsied. at necropsy, there was marked ...19827056675
two actinobacillus species isolated from laboratory rodents.a study was made of the cultural and biochemical characteristics of 17 strains of actinobacillus isolated from laboratory rats and hamsters. 2 types were recognized which differed in their urease reaction and colony colour on blood agar. the results obtained suggest that one was actinobacillus equuli but the other could not be identified as belonging to a described species. the characteristics of both organisms are such that they could be confused with pasteurella pneumotropica. one of the types ...19807188999
isolation of actinobacillus lignieresii and actinobacillus equuli from laboratory rodents.actinobacillus lignieresii and actinobacillus equuli were cultured from a total of 36 guinea pigs, rats, and mice. the organisms were isolated from the oropharynx, the conjunctiva, and middle ear. isolates were initially screened by eight biochemical tests to determine whether they were of the genus actinobacillus. actinobacillus spp. were then differentiated by fermentation reactions of nine carbohydrates. in the past, actinobacilli may have been mistakenly identified as pasteurella spp., espec ...19807217333
serum levels of amoxycillin following its oral administration to thoroughbred foals.amoxycillin trihydrate was administered orally to 6 foals at dose rates of 13 mg/kg (low), 20 mg/kg (medium) and 30 mg/kg (high) and serum concentrations determined at intervals up to 8 h. therapeutic serum levels of 1 microgram/ml persisted for 268 mins at a dose rate of 13 mg/kg, for 339 mins at 20 mg/kg and for 381 mins at 30 mg/kg. a 2 micrograms/ml serum level persisted for 198 mins at a dose rate of 13 mg/kg, for 268 mins at 20 mg/kg and for 311 mins at 30 mg/kg. to determine the spectra o ...19817238483
peritonitis in horses associated with actinobacillus equuli.actinobacillus equuli was the cause of peritonitis in 5 horses. in 3 the onset was sudden with intestinal stasis and acute abdominal pain as predominant findings. two others presented with chronic disease and weight loss. characteristically the peritoneal fluid had a high nucleated cell count with non-degenerate neutrophils as the predominant cell type. four horses were treated and recovered.19807425990
pleural effusion associated with acute and chronic pleuropneumonia and pleuritis secondary to thoracic wounds in horses: 43 cases (1982-1992).case records of 43 horses with pleural effusion associated with acute pleuropneumonia, chronic pleuropneumonia, or pleuritis secondary to a penetrating thoracic wound were reviewed to determine the predisposing factors, diagnosis, and treatment of this condition. acute pleuropneumonia was diagnosed in 36 horses, the majority of which were thoroughbreds (89%). of 22 (61%) horses that were in race training at the onset of illness, 11 (31%) had been recently transported a long distance and 4 (11%) ...19947744650
peritonitis associated with actinobacillus equuli in horses: 15 cases (1982-1992).peritonitis attributable to actinobacillus equuli was diagnosed in 15 horses examined at the veterinary center between 1982 and 1992. in 13 horses, historical findings included acute onset of mild to severe signs of abdominal pain, lethargy, and inappetence. two other horses had a history of weight loss for 3 to 6 weeks prior to examination. diagnosis was based on the physical signs and laboratory findings, including results of peritoneal fluid analysis (gross characteristics, total protein, tot ...19947928617
in-vitro susceptibility to antimicrobial drugs of bacterial isolates from horses in the netherlands.minimum inhibitory concentrations (mics) of 30 antimicrobial agents (including the hitherto unreported antimicrobial agents doxycycline, minocycline, vanomycin, 3 quinolones and 3 combinations of antimicrobial agents) for isolates of salmonella spp. (20), escherichia coli (17), klebsiella spp. (8), proteus spp. (7), pseudomonas aeruginosa (7), actinobacillus equuli (5), rhodococcus equi (4), streptococcus zooepidemicus (23), streptococcus equisimilis (6), streptococcus equi (4), coagulase-positi ...19938354217
a prospective study of septicaemia in colostrum-deprived foals.fourteen mares and their foals were attended at parturition. after mare-foal bonding, 8 colostrum-deprived (cd) foals were removed from their dams, deprived of colostrum, and provided with an alternative milk source for the first 24 h of life. the mares were milked out every 2-4 h during this period to remove colostrum, after which the cd foals were returned to their mares and allowed to nurse. six colostrum-fed (cf) foals were allowed to suck colostrum in the normal manner. foal serum igg conce ...19938508750
pharmacokinetics and therapeutic potential for repeated oral doses of trimethoprim/sulphachlorpyridazine in horses.the pharmacokinetic parameters of a powder formulation of trimethoprim/sulphachlorpyridazine were studied in eight healthy horses which received 5 mg/kg trimethoprim and 25 mg/kg sulphachlorpyridazine 12-hourly with concentrate for five days. the intake of the medicated concentrate by the horses was variable during the first two days, but after they became accustomed to the taste the intake by all the horses during the last three days was good. faecal samples taken before and on the last day of ...19958578661
equine neonatal septicaemia: 24 cases.equine neonatal septicaemia was confirmed in 24 foals hospitalised at the rural veterinary centre between 1989 and 1992 with suspected septicaemia. septicaemia was confirmed by culture of bacteria from blood of live foals and tissues obtained at necropsy of foals that died or were euthanased. pathogenic bacteria isolated were predominantly enterobacteriaceae (including escherichia coli and salmonella serovars) and actinobacillus equuli. clinical manifestations of septicaemia included signs of de ...19968660228
serological and biochemical properties of the major outer membrane protein within strains of the genus actinobacillus.sarcosyl-extracted outer membrane preparations of organisms of the genus actinobacillus were investigated with regard to heat-modifiable and serological properties as well as n-terminal amino acid sequencing of the isolated major outer membrane protein (omp). the major omp of actinobacillus lignieresii was recognized by a monoclonal antibody with specificity towards proteus mirabilis ompa. moreover, n-terminal amino acid sequencing revealed strong homology to ompa of enterobacteriaceae, on the c ...19968837386
detection of bacteria in equine synovial fluid by use of the polymerase chain reaction.equine synovial fluid aliquots were inoculated with salmonella enteritidis, escherichia coli, actinobacillus equuli, staphylococcus aureus, and streptococcus zooepidemicus to obtain approximate concentrations of 1000, 100, 10, and 1 colony forming u/ml. synovial fluid aliquots were also inoculated with an unquantitated inoculum of bacteroides fragilis and clostridium perfringens. inoculated synovial fluid was incubated in trypticase-soy broth or columbia broth for approximately 12 hours. then al ...19969012103
characterisation of australian isolates of actinobacillus capsulatus, actinobacillus equuli, pasteurella caballi and bisgaard taxa 9 and 11.the objective of this work was to perform a comprehensive phenotypic characterisation of 16 isolates of bacteria previously identified as actinobacillus equuli.19979034500
isolation of actinobacillus equuli from the oral cavity of healthy horses and comparison of isolates by restriction enzyme digestion and pulsed-field gel electrophoresis.swab samples were collected from the oral cavity of 174 horses in 10 farms and cultured selectively for actinobacillus equuli. a. equuli could be isolated from 37% of all samples, varying between 12 and 88% in the different farms. eight horses were sampled repeatedly for several days, with a variation in isolation frequency between 50 and 88%. isolates were compared by restriction enzyme digestion and pulsed-field gel electrophoresis. a high degree of strain variability was found within each hor ...19989549855
diversity among isolates of actinobacillus equuli and related organisms as revealed by ribotyping.the objective of this work was to examine the diversity within australian isolates of actinobacillus equuli and related organisms by the genotypic method of ribotyping.19989673769
actinobacillus equuli septicemia: an unusual zoonotic infection.we describe the isolation of actinobacillus equuli from the blood of a 53-year-old butcher with septicemia. this species of the genus actinobacillus is primarily associated with animals and animal diseases, especially septicemia in foals. this is the first report of the isolation of a. equuli from a human with septicemia.19989705442
serum antibody in equine neonatal septicaemia due to actinobacillus equuli. 19989773474
actinobacillus and pasteurella species isolated from horses with lower airway disease.seventy-three bacterial isolates from 65 horses with and without evidence of lower airway disease were identified to assess whether the association with disease was accounted for by a small or large number of species. just over half (50.5 per cent were actinobacillus equuli, 17.8 per cent were a suis-like, 11 per cent were pasteurella pneumotropica, 8.2 per cent were a lignieresii, 6.8 per cent were p haemolytica and 5.5 per cent were p mairii. these results suggest that a range of actinobacillu ...19989787421
molecular cloning and sequencing of the aroa gene from actinobacillus pleuropneumoniae and its use in a pcr assay for rapid identification.the gene (aroa) of actinobacillus pleuropneumoniae, serotype 2, encoding 5-enolpyruvylshikimate-3-phosphate synthase was cloned by complementation of the aroa mutation in escherichia coli k-12 strain ab2829, and the nucleotide sequence was determined. a pair of primers from the 5' and 3' termini were selected to be the basis for development of a specific pcr assay. a dna fragment of 1,025 bp was amplified from lysed a. pleuropneumoniae serotypes 1 to 12 of biovar 1 or from isolated dna. no pcr p ...199910203526
biochemical fingerprinting and ribotyping of isolates of actinobacillus equuli from healthy and diseased horses.a total of 112 isolates of actinobacillus equuli, including both clinical isolates and isolates from the oral cavity of healthy horses, were included in this study. all isolates were ribotyped and 92 of the isolates were also typed biochemically, with the commercially available pheneplate (php) system, which includes 48 different substrates. as expected, ribotyping was more sensitive than biochemical fingerprinting in detecting differences between the isolates. the correlation between the two me ...199910223322
effects of actinobacillus equuli culture supernatants on equine neutrophil functions and survival.after exposure of equine granulocytes from both foals and adult horses to culture supernatants from clinical isolates of actinobacillus equuli, phagocytic capacity and respiratory burst was examined by flow-cytometry and a chemiluminescence assay, respectively. one haemolytic isolate of an equine actinobacillus was also included in the study. an average decrease of 22% in total number of granulocytes, in the flow cytometric assay (p < 0.01), and an average decrease of 26% in light emission, in t ...199910605369
apx toxins in pasteurellaceae species from animals.pasteurellaceae species particularly of porcine origin which are closely related to actinobacillus pleuropneumoniae were analyzed for the presence of analogues to the major a. pleuropneumoniae rtx toxin genes, apxicabd, apxiica and apxiiicabd and for their expression. actinobacillus suis contains both apxicabd(var.suis) and apxiica(var. suis) operons and was shown to produce apxi and apxii toxin. actinobacillus rossii contained the operons apxiica(var.rossii) and apxiiicabd(var.rossii). however, ...200010831858
septicemia and peritonitis due to actinobacillus equuli infection in an adult horse.actinobacillus equuli is a rare cause of peritonitis in adult horses. septicemia and peritonitis due to a. equuli were diagnosed at necropsy in an 8-year-old saddlebred mare. the origin of the infection was not known; however, small necrotic colonic mucosal lesions presumed to have been caused by phenylbutazone treatment may have allowed bacterial invasion. a good response to antimicrobial treatment has been documented in the small numbers of previously reported acute cases of peritonitis. becau ...200111280382
pharmacokinetics of orbifloxacin and its concentration in body fluids and in endometrial tissues of mares.pharmacokinetics and distribution of orbifloxacin into body fluids and endometrium was studied in 6 mares after intragastric (ig) administration at a single dose rate of 7.5 mg/kg body weight. orbifloxacin concentrations were serially measured in serum, synovial fluid, peritoneal fluid, urine, cerebrospinal fluid, and endometrial tissues over 24 hours. minimum inhibitory concentrations of orbifloxacin were determined for 120 equine pathogens over an 11-month period. the mean peak serum concentra ...200111480524
peritonitis associated with actinobacillus equuli in horses: 51 cases.to review the clinical findings, diagnosis and treatment of 51 horses with peritonitis attributed to actinobacillus equuli.200111599812
opsonic capacity of foal serum for the two neonatal pathogens escherichia coli and actinobacillus equuli.two of the most commonly isolated foal pathogens are escherichia coli and actinobacillus equuli. the hypothesis tested in this study was that young foals carry a lower opsonic capacity for these bacteria compared to adult horses. a flow-cytometric method for the phagocytosis of these by equine neutrophils was established. the opsonic capacity of serum from healthy foals from birth to age 6 weeks was evaluated and related to the concentrations of igga and iggb. phagocytosis of yeast was used as a ...200111770988
predictive values, sensitivity and specificity of abdominal fluid variables in determining the need for surgery in horses with an acute abdominal crisis.to determine the predictive values, sensitivity and specificity of abdominal fluid variables associated with the need for surgery in horses with an acute abdominal crisis.200212019696
characterization of aqx and its operon: the hemolytic rtx determinant of actinobacillus equuli.actinobacillus equuli, a member of the family pasteurellaceae is the etiologic agent of a frequently lethal septicemia in neonatal foals as well as other more chronic diseases like arthritis, pleuritis, pneumonia or peritonitis. it may also be isolated from the oral cavity of healthy horses. hemolytic isolates of a. equuli are known but so far no virulence determinants have been described for this bacterial species. by screening hemolytic a. equuli strains with specific gene probes, a hemolysin, ...200212034544
final classification of bisgaard taxon 9 as actinobacillus arthritidis sp. nov. and recognition of a novel genomospecies for equine strains of actinobacillus lignieresii.phenotypic characterization of bacteria from diseased and healthy horses identified 18 isolates as bisgaard taxon 9 and 11 isolates as actinobacillus lignieresii. all strains of taxon 9 were alpha-galactosidase- and raffinose-positive and showed variable fermentation of (+)l-arabinose and (-)d-sorbitol. strains of a. lignieresii were negative for these characteristics, with the exception of raffinose. two strains from the (-)d-sorbitol-negative group of taxon 9 showed a 16s rrna similarity of 99 ...200212148635
reclassification of equine isolates previously reported as actinobacillus equuli, variants of a. equuli, actinobacillus suis or bisgaard taxon 11 and proposal of a. equuli subsp. equuli subsp. nov. and a. equuli subsp. haemolyticus subsp. nov.members of bisgaard taxon 11 have been isolated from horses. these bacteria are of importance in the veterinary clinic and also to the medical profession, since they may be isolated from infected wounds of humans bitten by horses. six strains from different continents were identified as taxon 11, with 16s rrna similarities between 98.0 and 99.7%. a single isolate that represented the so-called (+)l-arabinose-positive actinobacillus equuli isolated from a diseased foal showed 99.9% 16s rrna simil ...200212361259
host cell specific activity of rtx toxins from haemolytic actinobacillus equuli and actinobacillus suis.we assessed and compared host cell specificity of the haemolytic and cytotoxic activity of the rtx toxins from actinobacillus equuli, an equine pathogen, and actinobacillus suis, which is pathogenic for pigs. the two bacterial species are closely related, phenotypically as well as phylogenetically, sharing the same 16s rrna gene sequence. both species contain specific protein toxins from the family of pore-forming rtx toxins, however, the two species differ in their rtx toxin profiles. haemolyti ...200312488079
phylogenetic relationship of equine actinobacillus species and distribution of rtx toxin genes among clusters.equine actinobacillus species were analysed phylogenetically by 16s rrna gene (rrs) sequencing focusing on the species actinobacillus equuli, which has recently been subdivided into the non-haemolytic a. equuli subsp. equuli and the haemolytic a. equuli subsp. haemolyticus. in parallel we determined the profile for rtx toxin genes of the sample of strains by pcr testing for the presence of the a. equuli haemolysin gene aqx, and the toxin genes apxi, apxii, apxiii and apxiv, which are known in po ...200312791244
[pathogenic power of bacterium viscosum equi for small laboratory animals]. 195613327482
[somato-antigenic affinity of escherichia coli to shigella viscosa]. 195414391986
[shigella equirulis and bacterium anitratum (parashigellosis of foals)]. 195014775475
a medium for maintaining the viability of shigella equuli. 195114812836
delineation of the genus actinobacillus by comparison of partial infb sequences.a 426 bp fragment of infb, a housekeeping gene that encodes translation initiation factor 2, was sequenced from 59 clinical isolates and type strains of actinobacillus species and sequences were compared. partial sequences of 16s rrna genes were also obtained. by comparing infb sequences, actinobacillus pleuropneumoniae, actinobacillus equuli, actinobacillus suis, actinobacillus ureae, actinobacillus arthritidis, actinobacillus hominis and two unnamed genomospecies showed more than 85 % similari ...200415143001
antibodies to aqx toxin of actinobacillus equuli in horses and foals.actinobacillus equuli is found in the normal oral flora of horses, but has been associated with several diseases, and particularly with the usually fatal septicaemia in neonatal foals which is thought to be associated with a failure of the passive transfer of immunoglobulins via the colostrum. the aqx protein of a equuli, belonging to the rtx family of pore-forming toxins, is also cytotoxic to horse lymphocytes. the presence of antibodies to aqx was investigated in sera from individual horses fr ...200415384504
subacute bacterial endocarditis with pulmonary embolism in a horse associated with shigella equirulis. 195015434463
aroa gene pcr-rflp diversity patterns in haemophilus parasuis and actinobacillus species.the haemophilus parasuis aroa gene encodes 5-enolpyruvylshikimate-3-phosphate synthase and participates in the aromatic amino acids and the folic acid universal metabolic pathway of bacteria. the application of aroa-based pcr-rflp methodology yields a significant degree of diversity in h. parasuis and actinobacillus species. pcr amplification of the aroa gene rendered a 1,067-bp fragment in all 15 h. parasuis serovars, and also in actinobacillus pleuropneumoniae serotypes 1-12, actinobacillus li ...200615936788
haemolytic strains of actinobacillus equuli. 197216030623
antibacterial activity of cefquinome against equine bacterial pathogens.cefquinome is known for its use as an antibacterial drug in cattle and pigs. the objective of this study was to evaluate the antibacterial activity of cefquinome against equine pathogenic bacteria. the minimum inhibitory concentration (mic) of cefquinome was determined for a total of 205 strains, which had recently been isolated in europe from diseased horses (respiratory infection, foal septicaemia). the bactericidal activity was tested against 19 strains using the time killing method. the post ...200616455213
studies on rough and smooth variants of shigella equirulis (b. nephritidis-equi). 193216559587
classification of actinobacillus spp isolates from horses involved in mare reproductive loss syndrome.to identify actinobacillus spp isolates recovered from fetuses and pericardial fluid from horses affected with mare reproductive loss syndrome (mrls) and determine whether these bacterial species are the same as those isolated from clinically normal horses.200616881857
an unusual actinobacillus equuli strain isolated from a rabbit with tyzzer's disease.actinobacillus equuli was isolated in pure culture from the liver and lungs of an adult rabbit with tyzzer's disease (clostridium piliforme). based on the haemolytic features on blood agar plates, a positive reaction in the camp-test, hydrolysis of esculin, the inability to ferment l-arabinose, tdna-pcr and sequencing of the 16s rrna gene, the isolate was classified as a. equuli subsp. haemolyticus biovar 1. however, the aqxa gene, characteristic for haemolytic a. equuli strains, was not detecte ...200717482388
serum antibodies in mares and foals to actinobacillus equuli whole cells, outer membrane proteins, and aqx toxin.actinobacillus equuli is carried in the alimentary tract of mares and can cause severe septicemia of neonatal foals. a hemolytic subspecies, a. equuli subsp. haemolyticus, and a non-hemolytic subspecies, a. equuli subsp. equuli, have been identified. hemolytic strains produce the rtx toxin aqx. the purpose of this study was to demonstrate sequentially in two sets of mare-foal pairs antibodies to a. equuli whole bacterial cells, outer membrane proteins, and recombinant aqx and to compare the tran ...200717604847
fatal pulmonary hemorrhage associated with rtx toxin producing actinobacillus equuli subspecies haemolyticus infection in an adult horse.a case of fatal pulmonary hemorrhage in a 6-year-old american paint mare with a 2-week history of intermittent coughing, fever, and epistaxis is described. significant macroscopic abnormalities at postmortem examination were restricted to the respiratory system, and microscopically, severe pulmonary hemorrhage with suppurative bronchopneumonia was found. actinobacillus equuli subsp. haemolyticus was cultured from a transtracheal wash performed antemortem as well as from the lungs at necropsy. th ...200818182526
actinobacillus equuli subsp. equuli associated with equine valvular endocarditis.microbiological and pathological data from a case of equine valvular endocarditis are reported. limited information is available on the pathogenic potential of equine actinobacillus species as several strains originate from apparently healthy horses. after the establishment of two subspecies within this species, this seems to be the first report of an etiological association between a. equuli subsp. equuli and equine endocarditis. furthermore, new information on some phenotypical characteristics ...200718184417
facial cellulitis due to actinobacillus equuli infection in a neonatal foal. 200818344501
metritis, valvular endocarditis, and septicemia by actinobacillus equuli in a gilt in the united states.a 1-year-old pregnant yorkshire gilt was found dead with no previous clinical signs. gross findings included metritis, splenomegaly, and valvular endocarditis. bacterial endocarditis (in the mitral and tricuspid valves) and metritis with dissemination to multiple organs was diagnosed by using histologic examination. gram-negative coccobacillary organisms present in the valvular lesions were characterized as actinobacillus equuli by using polymerase chain reaction examination on formalin-fixed, p ...200818587096
a first report on the microbial colonisation of the equine oesophagus.based on cryo-sem, standard and high resolution tem, glycoconjugate histochemistry, and microbiological differentiation, the present study demonstrates the colonisation of the epithelium of the equine oesophagus with microorganisms. as particularly apparent using cryo-sem to illustrate natural conditions, the present microbiota were clearly dominated by bacteria, forming a one-layer system, as attached to and embedded in concentrated mannose/mannan substances covering the outer stratum corneal c ...201019942420
shigella equirulis infection in california. 194521005490
actinobacillus equuli as a primary pathogen in breeding sows and piglets.the death of over 300 sows in 2 months on a 3000 sow farrow-to-isowean operation in manitoba was attributed to infection with actinobacillus equuli. this pathogen commonly infects foals, and is rarely reported in swine. our report is the second recently published case of this pathogen in north american swine.201021286321
classification of avian haemolytic actinobacillus-like organisms (bisgaard taxon 26) associated with anseriforme birds as actinobacillus anseriformium sp. nov.avian haemolytic actinobacillus-like organisms have tentatively been named bisgaard taxon 26. phenotypic information has been published on 65 strains of this taxon. in the current study, 31 isolates were selected for genotypic characterization. thirty strains had the same rpob sequence and only one strain diverged in one nt. the highest rpob similarity to other taxa of 89.7 % was obtained to the type strain of actinobacillus equuli subsp. haemolyticus and the similarity to the type strain of the ...201121441378
serum antibody responses in horses and mice following immunization with actinobacillus equuli outer membrane proteins and recombinant aqx toxin.the immune responsiveness of mice (without prior natural exposure) and mares (with naturally acquired antibodies) was determined following vaccination with actinobacillus equuli outer membrane proteins (omps) and/or recombinant a. equuli toxin (raqx). mice were vaccinated subcutaneously on days 0 and 21 with one of three doses (5, 25 or 50μg) of a. equuli omps, raqx or both, together with freund's incomplete adjuvant (fia). antibodies against formalin-killed whole bacterial cells (wbcs), omps an ...201121596597
the role of rtx toxins in host specificity of animal pathogenic pasteurellaceae.rtx toxins are bacterial pore-forming toxins that are particularly abundant among pathogenic species of pasteurellaceae, in which they play a major role in virulence. rtx toxins of several primary pathogens of the family of pasteurellaceae are directly involved in causing necrotic lesions in the target organs. many rtx toxins are known as haemolysins because they lyse erythrocytes in vitro, an effect that is non-specific, but which serves as a useful marker in bacteriological identification and ...201121645978
deficiency of eosinophil peroxidase detected by automated cytochemistry. 20113122494
differential gallbladder contractility in fundal adenomyomatosis: demonstration by cholecystokinin cholescintigraphy.the cholecystokinin cholescintigraphic findings of fundal adenomyomatosis in a 29-yr-old male with severe post-prandial pain are presented. planar cholescintigraphy demonstrated a trilobed gallbladder contour. following the administration of 0.02 micrograms/kg of cholecystokinin at maximal gallbladder filling, fundal dyskinesia was observed. regional gallbladder ejection fractions were: whole gallbladder, 43%; proximal two-thirds of the gallbladder, 70%; and gallbladder fundus, 32%. first harmon ...20133668668
asymptotic relative efficiency. 20133717280
umbilical-cord insertion as source of pure fetal blood for prenatal diagnosis. 200187711
james r. atkinson (january 6, 1920 - february 12, 1978). 2004356827
grounds for divorce in england and wales--a social and demographic analysis. 20133700447
[change in the alkaline phosphatase activity of blood neutrophils in children with pneumonia]. 20144184662
discussion: oxidative disorders. 1998104556
the rubella immunity gap. 200187852
[treatment of herpes zoster with emetine]. 20164767947
[serum calcium levels in a population of 6048 men; variations with age and correlation with other biological data]. 20154432038
[the immunomodulation of the natural killer activity of the splenocytes in c3ha mice during hepatoma 22a growth].a single injection of c3ha mice with various immunomodulators-ds-rna, thymogene (tm) and cyclophosphamide (cy)--performed one day before transplantation of syngeneic hepatoma 22a cells led to a decrease in the tumor growth rate. the most prominent effect was found following the cy treatment. the nk cell activity estimated per spleen of mice treated with ds-rna and tm was seen increased in comparison with the control mice not given the modulators. the rate of tumor growth was due probably to this ...20072238117
[clothing]. 20154858032
report of a case: removal of an antral dentigerous cyst via a modified caldwell-luc. 1987265903
[plasma variation of atrial natriuretic peptide in central hypervolemia (diurnal or nocturnal) induced by antiorthostatic decubitus at -6 degrees].plasma levels of anp were measured during a 4 hrs head-down tilt at -6 degrees in 5 healthy male volunteers (aged 20-22 m.2). the experiments took place from 8 to 14 hrs, (day), and from 22 to 7 hrs (night). the control period was 1 hr. in a seated position (8 to 9 hrs. for day and 22 to 23 hrs. for night). blood samples were collected at 9 and 23 hrs. and every 20 min. during the first hours, and every hours thereafter. electroencephalograms were continuously recorded during night. our results ...20102957028
direct recovery of proteins into a free-liquid phase after preparative isoelectric focusing in immobilized ph gradients.a new method for electrophoretic retrieval of protein zones from immobiline matrices is described, based on elution directly in a free liquid phase, rather than in ion-exchange beads or molecular sieves, as previously described. the chopped immobiline gel is loaded on top of a 5% t stacking gel, 6-10 mm in height, and forced to transverse it and collect into a chamber, filled with 20% sucrose solution, closed on its anodic side by a dialysis sac. the transfer is practically quantitative, for mos ...20133782719
[apprenticeship--a hard time--reliability]. 20154511653
[the days of the battle of moscow (from the memoirs of the military veterinary service of the western front)]. 20154895403
first human case of meningitis and sepsis in a child caused by actinobacillus suis or actinobacillus equuli.we report the first human case of meningitis and sepsis caused in a child by actinobacillus suis or a. equuli, a common opportunistic pathogen of swine or horses, respectively. identification was performed by matrix-assisted laser desorption ionization-time of flight mass spectrometry and real-time pcr assay. a previous visit to a farm was suspected as the source of infection.201525878346
complete genome sequence of actinobacillus equuli subspecies equuli atcc 19392(t).actinobacillus equuli subsp. equuli is a member of the family pasteurellaceae that is a common resident of the oral cavity and alimentary tract of healthy horses. at the same time, it can also cause a fatal septicemia in foals, commonly known as sleepy foal disease or joint ill disease. in addition, a. equuli subsp. equuli has recently been reported to act as a primary pathogen in breeding sows and piglets. to better understand how a. equuli subsp. equuli can cause disease, the genome of the typ ...201526203343
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