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isolation of actinobacillus suis from a colt. 1978630374
morphologic and functional characteristics of human intestinal lymphoid cells isolated by a mechanical technique. 1979153255
effect of spa waters from the hisar health resort springs "momina banja" and "momina salza" on experimentally induced gastric ulcer. 1978104904
lysosomal acid hydrolases of normal adipose tissues of man, monkey and rat. 197897094
actinobacillus suis infections in alberta swine, 1973--75: pathology and bacteriology. 1976974978
actinobacillus suis in the horse. 19761265987
infection with actinobacillus suis in pigs. 1978696543
alteration of lung structure and function by pseudomonas aeruginosa. 19873122195
opsonization of yeast by the plasma of biomphalaria glabrata (gastropoda): a strain-specific, time-dependent process.phagocytosis of yeast by haemocytes of biomphalaria glabrata, intermediate host of schistosoma mansoni, is influenced strongly by host plasma components, although it can occur without involvement of such factors. plasma from two strains of b. glabrata which are resistant to s. mansoni differs in its opsonic properties from the plasma of a susceptible strain. this may reflect the principles which determine compatibility or incompatibility in this host-parasite system. opsonization is a time-depen ...19892671868
suppression of sv40-promoted gene expression by differentiation of preadipose cells.when a plasmid bearing the chloramphenicol acetyltransferase (cat) gene under the control of an sv40 early promoter is introduced into preadipose or adipose cells of line 3t3-f442a, the promoter directs high levels of transient expression of cat. however, when the plasmid is introduced into preadipose cells and the cells are then allowed to differentiate into adipose cells, the expression of the cat gene is suppressed. in this process, the plasmid is not changed detectably in amount, topology, o ...19882849579
prevalence of seroreactors to the 104-kilodalton hemolysin of actinobacillus pleuropneumoniae in swine herds.a rabbit homologous polyclonal antiserum to the 104-kilodalton hemolysin of actinobacillus pleuropneumoniae serotype 1 strain cm-5 was specifically produced and used in an antigen capture enzyme-linked immunosorbent assay (elisa) to detect swine serum antibodies to this potentially important virulence factor. sera from pigs experimentally infected with the most common disease-producing serotypes (serotypes 1, 2, 5, and 7) of a. pleuropneumoniae produced positive results in this elisa. of 144 ser ...19902332472
malignant hyperpyrexia. 19892653537
very early aortic responses during atherosclerosis induction in rabbits: measurement by duplex ultrasound. i. non-invasive study of aortic hyperresponsiveness to serotonin.we report the use of a non-invasive ultrasound method to visualize and measure changes in serotonin reactivity of the abdominal aorta during the early stages of atherosclerosis development. studies were performed at 0, 4, 8, and 14 weeks in new zealand white rabbits fed a diet enriched with 0.5% cholesterol. change in systolic vessel diameter at each examination was compared with changes in rabbits fed a control diet or rabbits on a diet enriched with cholesterol plus a concentrated marine lipid ...19902202316
serodiagnosis of pleuropneumonia using enzyme-linked immunosorbent assay with capsular polysaccharide antigens of actinobacillus pleuropneumoniae serotypes 1, 2, 5 and 7.capsular polysaccharide antigens of serotypes 1, 2, 5 and 7 of actinobacillus pleuropneumoniae were used in enzyme-linked immunosorbent assays (elisas) to test sera from experimentally infected and field pigs. specific reactions were found in sera of experimental pigs with antigens of serotypes 1, 5 and 7 whereas the serotype 2 antigen was cross-reactive. a 1:200 serum dilution was used for testing of 300 sera from 21 swine herds in southern ontario. cases of pleuropneumonia had occurred in 11 o ...19902249177
further evidence for angiotensin-sodium interaction in central control of fluid balance. 19704320106
comparison of ascorbic acid and ascorbic acid 2-o-alpha-glucoside on the cytotoxicity and bioavailability to low density cultures of fibroblasts.ascorbic acid 2-o-alpha-glucoside (aa-2g) is a stable ascorbate derivative which has vitamin c activity in vivo and in vitro. we studied whether aa-2g exerts a prooxidant action in cultured fibroblasts from chick embryo and human skin, as does ascorbic acid. at concentrations of 0.1-1.0 mm, ascorbic acid markedly reduced the viable cell number of low density cultures within 24 hr, whereas aa-2g had no such effect. the ascorbate cytotoxicity was dependent on the cell density at the time of its ad ...19921472084
molecular characterization of an rtx toxin determinant from actinobacillus suis.rtx cytolysins are a family of calcium-dependent, pore-forming, secreted toxins found in a variety of gram-negative bacteria. the prototypical member of this family is the alpha-hemolysin of escherichia coli. the rtx genetic determinants from seven members of the family pasteurellaceae, pasteurella haemolytica, actinobacillus actinomycetemcomitans, and a. pleuropneumoniae serotypes 1,5,7, and 9 were previously cloned and sequenced. using the leukotoxin determinant from p. haemolytica serotype a1 ...19921587585
thermogenesis and mitochondrial gdp binding with age in response to the novel agonist cgp-12177a.the ability to regulate body temperature diminishes with age in both humans and rodents. to investigate whether attenuation of sympathetically activated thermogenesis in brown adipose tissue (bat) may account for the loss of thermoregulation with age, we assessed o2 consumption and body temperature in response to norepinephrine and the specific bat beta-adrenergic agonist cgp-12177a in 6-, 18-, and 24-mo-old rats. in addition, the effects of this agonist on interscapular bat mitochondrial gdp bi ...19921539643
an environmental chamber system for prolonged metabolic studies on small animals. 19734771134
proceedings: uptake and incorporation of (u-14c)glucose during culture of mouse blastocysts previously undergoing normal implantation and induced delay of implantation. 19761255628
morphological and functional aspects of the glomerular basement membrane.addition of alcian blue to the fixative reveals structural elements bearing negatively charged sites within the glomerular capillary wall of the rat kidney. according to previous research (review by brenner et al., 1977), these charges represent one of the most important functional components of the glomerular filtration barrier. alcian blue also demonstrates negatively charged microfibrils (probably glycoproteins) of the mesangial matrix. preliminary observations of glomeruli of lower vertebrat ...197995429
interrupting the early region of polyoma virus dna enhances tumorigenicity.the tumorigenicity of dna from polyoma virus after cleavage with a variety of restriction enzymes was evaluated in suckling hamsters. cleavage with enzymes that interrupt the region of the genome coding for the large tumor (t) antigen of polyoma virus markedly enhanced the tumorigenicity above that observed with dna i of the virus. cell lines established in vitro from tumors induced by polyoma virions, polyoma virus dna i, or polyoma virus dna that had been cleaved with restriction endonucleases ...1979226975
qualitative and quantitative determination of enterobacterial common antigen (eca) with monoclonal antibodies: expression of eca by two actinobacillus species.the presence and quantity of the enterobacterial common antigen (eca) in several species belonging to the family enterobacteriaceae as well as to other gram-negative families were determined by a solid-phase enzyme-linked immunosorbent assay system and western blotting by using mouse monoclonal antibodies specific for eca. except for erwinia chrysanthemi, previously known to be an exception, all species known or presumed to belong to enterobacteriaceae produced eca (89 of 90 species). most speci ...19873818929
isolation of actinobacillus suis from a canada goose (branta canadensis).blindness from conjunctivitis caused by actinobacillus suis was found in a canada goose (branta canadensis) from a wildlife refuge in kentucky. lesions were not observed elsewhere and other body organs were grossly normal. actinobacillus spp. in birds is rare and this is apparently the first report of conjunctivitis resulting from this infection in waterfowl.19873625911
enhancement of host resistance to bacterial infections in normal and immunosuppressed mice with actinobacillus suis.a single intraperitoneal (ip) inoculation of heat-killed actinobacillus suis atcc 15,557 (as 15,557) into normal and immunosuppressed (dexamethasone-treated) mice led to remarkable nonspecific resistance to ip challenge with lethal doses of opportunistic pathogens such as pseudomonas aeruginosa, methicillin-resistant staphylococcus aureus and candida albicans. the duration of this enhanced protective action and the minimal effective dose, in normal mice, induced by as 15,557 were superior to tho ...19968741706
actinobacillus suis-like organisms in horses.actinobacillus suis-like organisms have been recognized in equine specimens at the university of california veterinary medical teaching hospital since 1975. the most common source (65%) of the organism was transtracheal washings. the organism was gram-negative, produced hemolysis on blood agar, and gave a positive reaction for oxidase, urease, o-nitrophenyl-beta-d-galactopyranoside, and esculin. carbohydrate reactions were variable, consisting of 4 main patterns. actinobacillus suis-like organis ...19873631683
acute interstitial pneumonia [proceedings]. 1977193807
evaluation of heat-sensitive, neutrophil-toxic, and hemolytic activity of haemophilus (actinobacillus) pleuropneumoniae.cytotoxic and hemolytic activity of haemophilus (actinobacillus) pleuropneumoniae serotype 1 strain cm5 was investigated because of the potential role as a virulence determinant. viable bacteria were toxic for porcine and bovine neutrophils, whereas bacteria killed by heat treatment at 60 c for 1 hour were not. similarly, bacteria-free culture supernatant was cytotoxic and hemolytic in assays that used porcine neutrophils and erythrocytes, whereas supernatant treated at 60 c for 1 hour had no ac ...19883421527
multiple cis-acting sequence elements are required for efficient splicing of simian virus 40 small-t antigen pre-mrna.we have determined the effects of a number of mutations in the small-t antigen mrna intron on the alternative splicing pattern of the simian virus 40 early transcript. expansion of the distance separating the small-t pre-mrna lariat branch point and the shared large t-small t 3' splice site from 18 to 29 nucleotides (nt) resulted in a relative enhancement of small-t splicing in vivo. this finding, coupled with the observation that large-t pre-rna splicing in vitro was not affected by this expans ...19882851720
immunoserological comparison of 104-kilodalton proteins associated with hemolysis and cytolysis in actinobacillus pleuropneumoniae, actinobacillus suis, pasteurella haemolytica, and escherichia coli.a homologous polyclonal antibody was produced in a rabbit to the 104-kilodalton (kda) protein hemolysin of actinobacillus pleuropneumoniae serotype 1 strain cm-5. in immunoblots, this antibody recognized a similar 104-kda protein produced in culture supernatants by a. pleuropneumoniae serotypes 1 to 12 and taxon "minor group" in addition to pasteurella haemolytica, actinobacillus suis, and alpha-hemolysin-producing escherichia coli (but only weakly in the latter two organisms). these results wer ...19892674017
similarity principles and intrinsic geometries: contrasting approaches to interspecies scaling.we criticize standard allometric approaches on the grounds that they emphasize scaling to one variable at a time, whereas chemically reactive hydrodynamic systems involved in pharmacokinetic phenomena are of higher dimension. we show that attempts based on mechanical similitude to set a dosage that would be equivalent across species (for example, from mouse to humans) lead to ambiguous results. another failing of standard allometry may be its incapability to accommodate the neoteny of homo sapie ...19863820095
analysis of major antigens of haemophilus (actinobacillus) pleuropneumoniae and related organisms.outer membrane protein (omp)-enriched extracts and whole-cell protein preparations of haemophilus (actinobacillus) pleuropneumoniae and related organisms were examined by polyacrylamide gel electrophoresis and immunoblotting. both the omp-enriched and whole-cell protein profiles of actinobacillus suis, a. pleuropneumoniae (nad-independent biovar), a. lignieresii, and pasteurella haemolytica were very similar to those of h. pleuropneumoniae serotypes 1 to 8. antisera prepared against h. pleuropne ...19873298061
cefadroxil in the horse: pharmacokinetics and in vitro antibacterial activity.sodium cefadroxil was administered as a single intravenous dose (25 mg/kg) to six healthy adult mares. plasma samples were collected over a 24-h period and cefadroxil concentrations were measured by microbiological assay. the pharmacokinetic behavior of the drug was appropriately described in terms of a one-compartment open model. values for the major pharmacokinetic terms were: extrapolated initial plasma concentration = 59.2 +/- 15.0 micrograms/ml; half-life = 46 +/- 20 min; apparent volume of ...19854057345
[respiratory diseases of swine: some epidemiologic aspects].epidemiological surveys enable to know better the aetiology of respiratory diseases. the surveys carried out in slaughter-houses in brittany (france) in 1980 and 1981, show a worrying situation concerning porcine respiratory diseases. microbiological studies brought out the preponderance of the isolation of mycoplasma hyopneumoniae, pasteurella multocida, bordetella bronchiseptica, streptococcus suis and actinobacillus suis. some patterns of experimental infection with mycoplasma hyopneumoniae a ...19854064626
in vitro susceptibility of selected veterinary bacterial pathogens to ciprofloxacin, enrofloxacin and norfloxacin.the minimum inhibitory concentrations (mic) of ciprofloxacin, enrofloxacin, and norfloxacin were tested for approximately ten clinical isolates of each of actinobacillus pleuropneumoniae, actinobacillus suis, actinomyces pyogenes, corynebacterium pseudotuberculosis, erysipelothrix rhusiopathiae, haemophilus parasuis, haemophilus somnus, pasteurella haemolytica, pasteurella multocida, rhodococcus equi, streptococcus equi, streptococcus suis and streptococcus zooepidemicus. ciprofloxacin and enrof ...19902306672
monoclonal antibody in the identification of haemophilus somnus.electrophoretic comparisons of outer membrane proteins of haemophilus somnus isolates revealed 2 major protein bands (46 and 14 kilodaltons [kd]) common to all isolates tested. a monoclonal antibody raised against h. somnus reacted to the 46-kd band. coagglutination tests were performed using a monoclonal antibody coagglutination assay. the monoclonal reagent was produced by incubating cowan strain staphylococcus aureus suspension, used as a source of crude protein a, with mouse ascitic fluid mo ...19902094432
isolation of actinobacillus suis from a cat's lung.actinobacillus suis has been isolated from the lungs of 9-month-old cat. the bacterium was characterized biochemically as well as genetically, and its sensitivity profile to different antimicrobial agents was established. the role of this isolate in the cat's condition is discussed.19999919368
the cytotoxin of actinobacillus pleuropneumoniae (pleurotoxin) is distinct from the haemolysin and is associated with a 120 kda polypeptide.mutants of actinobacillus pleuropneumoniae strain hk 361 (serotype 2) were isolated which were deficient in type ii (ca2(+)-dependent) haemolysin activity (hly-). some of the hly- mutants secreted a potent, heat-labile extracellular cytotoxic activity against porcine alveolar macrophages. comparison of cell-free culture supernatant from the parent strain and some hly- mutants by sds-page and immunoblotting revealed the loss of a major extracellular polypeptide of 109 kda. two hly- mutants which ...19912033378
simplified procedure for preparation of sensitized latex particles to detect capsular polysaccharides: application to typing and diagnosis of actinobacillus pleuropneumoniae.a novel, inexpensive method for obtaining immunoglobulin g (igg) specific for capsular antigen is described for use in latex agglutination tests. hyperimmune rabbit serum against encapsulated actinobacillus pleuropneumoniae was thoroughly adsorbed with a nonencapsulated mutant. the capsule titer of the absorbed serum was unaffected, whereas reactivity to nonencapsulated cells was reduced to background levels, as determined by enzyme immunoassay. the igg component of the adsorbed serum was recove ...19957494018
actinobacillus suis-like organisms and evidence of hemolytic strains of actinobacillus lignieresii in horses.thirty-seven local isolates of actinobacillus suis-like organisms from diseased and clinically normal horses and 1 llama were compared with reference strains of a suis, a lignieresii, a equuli, a capsulatus, a hominis, a (pasteurella) ureae, and equine a suis-like organisms (aslo) previously described in literature. comparison was by cultural characteristics, carbohydrate fermentation, enzyme profiles, and whole-cell protein polyacrylamide gel electrophoresis. carbohydrate fermentation, determin ...19911928905
pasteurella multocida produces a protein with homology to the p6 outer membrane protein of haemophilus influenzae.an antibody specific for a 16-kda outer membrane protein of a rabbit strain of pasteurella multocida was used to probe representatives of all 16 somatic serotypes of p. multocida, as well as the vaccine strains cu and m9, and all were shown to express the protein. the gene encoding this protein was cloned and sequenced and found to have extensive sequence homology with the gene encoding the p6 protein of haemophilus influenzae. the protein in p. multocida has been designated p6-like. the gene en ...19957868272
evaluation of a saline boiled extract, capsular polysaccharides and long-chain lipopolysaccharides of actinobacillus pleuropneumoniae serotype 1 as antigens for the serodiagnosis of swine pleuropneumonia.a saline boiled extract (sbe), capsular polysaccharides (cps) and long-chain lipopolysaccharides (lc-lps) of actinobacillus pleuropneumoniae serotype 1 have been evaluated in elisa for the serodiagnosis of swine pleuropneumonia caused by this serotype. mean optical densities (ods) obtained with the three antigens using sera from negative herds as well as from animals experimentally and naturally exposed to a. pleuropneumoniae serotypes 1, 9 or 11 were not significantly different. the positive el ...19947886937
immunological properties of actinobacillus pleuropneumoniae hemolysin i.the 105 kda hemolysin i protein from actinobacillus pleuropneumoniae serotype i type strain 4074 (hlyi) was shown by immunoblot analysis to be the predominant immunogenic protein if convalescent field sera or sera from pigs experimentally infected with a. pleuropneumoniae serotype 1 were used. sds gel- and immunoblot-analysis using total culture, washed cells or culture supernatant showed that hlyi is essentially secreted and is not found attached to the bacteria. proteins in the 105 kda range t ...19911887566
the distribution of smooth muscle in the cat ovary with a note on its adrenergic innervation. 19734722569
[expression and distribution of catechol 2,3-dioxygenase in escherichia coli].a series of new plasmids containing xyle gene was constructed based on the shuttle plasmid ptg 402 between e. coli and b. subtilis. the expressed xyle gene product catechol 2,3-dioxygenase (cato2ase) was measured for its output and distribution, and analysed for its structural hydrophobicity and hydrophilicity. it was demonstrated that the output of cato2ase was relative to plasmids, host cells, culture time and with or without induction. the enzyme didn't have features of excretional proteins a ...19921524847
actinobacillus suis infection after a pig bite. 19968826821
serological and biochemical properties of the major outer membrane protein within strains of the genus actinobacillus.sarcosyl-extracted outer membrane preparations of organisms of the genus actinobacillus were investigated with regard to heat-modifiable and serological properties as well as n-terminal amino acid sequencing of the isolated major outer membrane protein (omp). the major omp of actinobacillus lignieresii was recognized by a monoclonal antibody with specificity towards proteus mirabilis ompa. moreover, n-terminal amino acid sequencing revealed strong homology to ompa of enterobacteriaceae, on the c ...19968837386
production of apx toxins by field strains of actinobacillus pleuropneumoniae and actinobacillus suis.the three apx toxins of actinobacillus pleuropneumoniae have potential value for use in vaccines and diagnostic tests which will be species specific instead of serotype specific, provided that the apx toxins are species specific and all field strains produce these toxins. we examined 114 a. pleuropneumoniae field strains and found that they secreted either apxi, apxii, apxi and apxii, or apxii and apxiii and secreted no other cytolytic activities. however, proteins similar to apxi and apxii were ...19948063425
biochemical and antigenic relationships between porcine and equine isolates of actinobacillus suis.a total of 50 actinobacillus suis isolates were studied for their biochemical and antigenic characteristics. of them, 40 isolates originated from different tissues of diseased pigs, and the other ten isolates were from horses with respiratory problems. there was no major biochemical difference among equine and porcine a. suis isolates. results of tube agglutination tests showed that porcines isolates were antigenically homogeneous while equine isolates were heterogeneous.19968870199
actinobacillus suis strains isolated from healthy and diseased swine are clonal and carry apxicabdvar. suis and apxiicavar. suis toxin genes.actinobacillus suis isolates recovered from both healthy and diseased pigs were characterized by biochemical testing, serotyping, restriction endonuclease fingerprinting, and apx toxin gene typing. the clinical isolates analyzed were collected over a 10-year period from approximately 40 different locations in southwestern ontario, canada. little variation in the biochemical profiles of these isolates was seen, and all isolates reacted strongly with rabbit antisera prepared against one of the str ...19979114394
actinobacillus suis-like organisms associated with septicemia in neonatal calves. 19968744750
molecular characterization of a common 48-kilodalton outer membrane protein of actinobacillus pleuropneumoniae.previous studies have shown that a vaccine prepared from outer membranes of actinobacillus pleuropneumoniae serotype 5 can elicit protective immunity in swine against challenge with either serotype 5 or serotype 1. these results suggest the presence of common subcapsular surface antigens, such as outer membrane proteins, that contribute to cross-protective immunity. we have identified a 48-kda outer membrane protein that is common to all 12 capsular serotypes of a. pleuropneumoniae but is not pr ...19968557378
an outbreak of actinobacillus suis septicemia in grow/finish pigs. 19968844585
[histoenzymology of the heart conduction system in myocardial infarct in man]. 19744431148
actinobacillus and streptococcus: producers of isoschizomers of the restriction endonucleases r.hphi, r.saui, r.nhei, r.mboi and r.swai.new restriction endonucleases have been found in microorganisms isolated from the microflora of human teeth. the strain-producers are actinobacillus suis and streptococcus milleri. the new enzymes are isoschizomers of the prototypes as follows: asuhpi - hphi; asusai - saui; asunhi - nhei; asumbi and smimbi - mboi; smii - rare-cutter swai.19989628357
differentiation of actinobacillus pleuropneumoniae strains by sequence analysis of 16s rdna and ribosomal intergenic regions, and development of a species specific oligonucleotide for in situ detection.the aims of this study were to characterize and determine intraspecies and interspecies relatedness of actinobacillus pleuropneumoniae to actinobacillus lignieresii and actinobacillus suis by sequence analysis of the ribosomal operon and to find a species-specific area for in situ detection of a. pleuropneumoniae. amplification and sequence analysis of the 16s-23s rdna ribosomal intergenic sequence (ris) from the three species showed the existence of two ris's, differing by about 100 bp. both se ...19989779607
agents of the "suis-ide diseases" of swine: actinobacillus suis, haemophilus parasuis, and streptococcus suis.in recent years, actinobacillus suis, haemophilus parasuis, and streptococcus suis have emerged as important pathogens of swine, particularly in high health status herds. their association with a wide range of serious clinical conditions and has given rise to the moniker "suis-ide diseases." these organisms are early colonizers and, for that reason, are difficult to control by management procedures such as segregated early weaning. vaccination, serodiagnostic testing, and even serotyping are com ...199910369563
comparative pathogenicity of different actinobacillus suis o/k serotypes.the pathogenicity of actinobacillus suis serotypes o1/k1 (strain so4), o1/k2 (strain c84), and o2/k2 (strain h91-0380) was evaluated in specific-pathogen-free (spf) piglets challenged by intraperitoneal inoculation with approximately 1 x 10(7) colony-forming units per ml. all 3 strains produced peritonitis, but differences were observed in the composite histopathologic scores (p = 0.001) and in their ability to spread (p = 0.008) at 7 h post challenge. the o2/k2 strain caused the most severe per ...200010805245
pathogenesis and clinical signs of equine herpesvirus-1 in experimentally infected ponies in vivo.equine herpesvirus-1 (ehv-1) causes respiratory disease, neonatal death, abortion and neurologic disease. the main purpose of this study was to identify viral antigen in respiratory tract samples by immunoperoxidase staining. six pony foals were selected on the basis of demonstrating seronegativity to ehv-1 by virus neutralization and housed in isolation. they were infected experimentally by administering ehv-1 nebulized ultrasonically through a face mask. successful infection was clinically app ...19989442940
synergistic effects of actinobacillus suis cells in combination with 5-fluorouracil on mice bearing murine l1210 leukemia cells.the mean survival rates of female bdf1 mice transplanted intravenously (i.v.) with murine l1210 leukemia cells were significantly prolonged by intraperitoneal (i.p.) pretreatment (before i.v. transplantation) or by i.p. pre- and post-treatment (before and after the i.v. transplantation) with heat-killed actinobacillus suis cells atcc 15557 (as 15557) alone, as compared with untreated l1210-leukemia-cell-bearing control mice. however, significant prolongation of the mean survival rates was not el ...19979222280
a 24-kda cloned zinc metalloprotease from actinobacillus pleuropneumoniae is common to all serotypes and cleaves actin in vitro.actinobacillus pleuropneumoniae causes pleuropneumonia in swine. this bacterium secretes proteases that degrade porcine hemoglobin and iga in vitro. to further characterize a. pleuropneumoniae proteases, we constructed a genomic library expressed in escherichia coli dh5alpha, and selected a clone that showed proteolytic activity. the recombinant plasmid carries an 800-base pair a. pleuropneumoniae gene sequence that.codes for a 24-kda polypeptide. a 350-base pair psti fragment from the sequence ...200010805246
actinobacillosis in an alpaca (lama pacos).the death of a 6-week-old alpaca being treated for acute polyarthritis is described. post-mortem and histological findings included fibrinous mural endocarditis, lung abscessation, acute passive congestion of the liver, embolic suppurative nephritis and chronic nonsuppurative polyarthritis. actinobacillus suis was isolated from the heart lesion and in pharyngeal swabs from four of six clinically normal alpacas in the same flock.199216031654
apx toxins in pasteurellaceae species from animals.pasteurellaceae species particularly of porcine origin which are closely related to actinobacillus pleuropneumoniae were analyzed for the presence of analogues to the major a. pleuropneumoniae rtx toxin genes, apxicabd, apxiica and apxiiicabd and for their expression. actinobacillus suis contains both apxicabd(var.suis) and apxiica(var. suis) operons and was shown to produce apxi and apxii toxin. actinobacillus rossii contained the operons apxiica(var.rossii) and apxiiicabd(var.rossii). however, ...200010831858
actinobacillus suis infection in pigs. a report of four outbreaks and two sporadic cases. 19744850396
prevalence of o1/k1- and o2/k3-reactive actinobacillus suis in healthy and diseased swine.a cell surface antigen-typing system was devised for the swine pathogen actinobacillus suis and used to examine the prevalence of different lipopolysaccharide (o) types in healthy and diseased pigs. the strains examined in this study were isolated from a variety of locations in canada and from kansas. lipopolysaccharide preparations of 151 isolates of a. suis were characterized by immunoblotting using polyclonal antisera generated to strains so4 (o1/k1), h89-1173 (o2/k3), and vsb 3714, a rough s ...200011015398
detection and subtyping of actinobacillus pleuropneumoniae strains by pcr-rflp analysis of the tbpa and tbpb genes.a pcr-based procedure for detection and serotype identification of actinobacillus pleuropneumoniae strains was developed and evaluated. the a. pleuropneumoniae tbpa and tbpb genes were used as targets for amplification of dna fragments, with a pair of specific primers for each gene. amplification with tbpa primers rendered a 2.8-kb pcr product from all 12 a. pleuropneumoniae reference strains as well as from actinobacillus suis strain ccm 5586, while amplification of a 1.9-kb pcr product was obs ...200011081581
the first description of a (1--> 6)-beta-d-glucan in prokaryotes: (1 --> 6)-beta-d-glucan is a common component of actinobacillus suis and is the basis for a serotyping system.the chemical and antigenic properties of the cell-surface lipopolysaccharides (lpss) and capsular polysaccharides (cpss) of seven representative strains of actinobacillus suis from healthy and diseased pigs were investigated. four strains produced a linear (1 --> 6)-beta-d-glucan homopolymer, beta-d-glcp-(1-[ --> 6)-beta-d-glcp-(1-]n -->, as a lps-o-chain (o1) and as a cps (k1). polyclonal antisera prepared against a (1 --> 6)-beta-d-glucan-containing strain showed a positive reaction against bo ...200011086692
the transferrin receptor of actinobacillus pleuropneumoniae: quantitation of expression and structural characterization using a peptide-specific monoclonal antibody.when actinobacillus pleuropneumoniae (a. pp) is grown under iron-restricted conditions in vitro, transferrin binding proteins (tbps) are induced. the functional transferrin receptor of a. pp is composed of two outer membrane proteins (tbp1 and tbp2) and shows an exquisite specificity for porcine transferrin. this complex was studied using a monoclonal antibody (mab 1.48) raised against a synthetic peptide corresponding to a hydrophilic domain of tbp2 common to several a. pp serotypes. the antibo ...200111356318
actinobacillus suis septicaemia in two foals.a 24-hour-old hackney ony filly developed signs of weakness, depression and a poor suck reflex, with harsh lung sounds over both fields, and a 48-hour-old arabian colt from a normal birth which had sucked vigorously developed loose stools and became depressed, weak and anorectic. both foals had serum igg concentrations greater than 800 mg/dl, but each had a severe neutropenia with a left shift, and blood cultures from both of them yielded actinobacillus suis. the a suis isolates had different an ...19968629322
in vitro susceptibility of porcine respiratory pathogens to tilmicosin.bacterial isolates obtained from swine with various clinical diseases were tested for susceptibility to tilmicosin by minimum inhibitory concentration (mic) and kirby-bauer disk diffusion tests using national committee on clinical laboratory standards methodology. the tilmicosin mic90 was < or =0.125 microg/ml for erysiopelothrix rhusiopathiae, < or = 1 microg/ml for haemophilus parasuis isolates, 8 microg/ml for actinobacillus suis and pasteurella multocida type a, 16 microg/ml for toxigenic an ...200011108454
reclassification of equine isolates previously reported as actinobacillus equuli, variants of a. equuli, actinobacillus suis or bisgaard taxon 11 and proposal of a. equuli subsp. equuli subsp. nov. and a. equuli subsp. haemolyticus subsp. nov.members of bisgaard taxon 11 have been isolated from horses. these bacteria are of importance in the veterinary clinic and also to the medical profession, since they may be isolated from infected wounds of humans bitten by horses. six strains from different continents were identified as taxon 11, with 16s rrna similarities between 98.0 and 99.7%. a single isolate that represented the so-called (+)l-arabinose-positive actinobacillus equuli isolated from a diseased foal showed 99.9% 16s rrna simil ...200212361259
prevalence of a novel capsule-associated lipoprotein among pasteurellaceae pathogenic in animals.capsular serotype a strains of pasteurella multocida of avian origin express a 40-kda lipoprotein (plp-40) thought to attach the extracellular polysaccharide to the cell surface. the objective of the present study was to assess the prevalence of plp-40 in p. multocida strains of disparate serotypes and host origins, as well as other pathogenic members of the family pasteurellaceae. exponential-phase reference and clinical isolates were radiolabeled with [3h]-palmitate, lysed to obtain whole-cell ...200211910502
iron acquisition by actinobacillus suis: identification and characterization of transferrin receptor proteins and encoding genes.actinobacillus suis is an important pathogen of swine, especially in high-health-status herds. a published report mentioning the binding of porcine transferrin (tf) by at least one strain of a. suis suggested that a. suis, like other members of the pasteurellaceae, can acquire tf-bound iron by means of a siderophore-independent, receptor-mediated mechanism. the objective of the present study was to characterize the components involved in this process, if present. growth assays, with seven strain ...200312742718
acute hemorrhagic pulmonary infarction and necrotizing pneumonia in horses: 21 cases (1967-1993).to characterize history, clinical signs, and pathologic findings in horses with histologically confirmed acute hemorrhagic pulmonary infarction and necrotizing pneumonia.19979187729
host cell specific activity of rtx toxins from haemolytic actinobacillus equuli and actinobacillus suis.we assessed and compared host cell specificity of the haemolytic and cytotoxic activity of the rtx toxins from actinobacillus equuli, an equine pathogen, and actinobacillus suis, which is pathogenic for pigs. the two bacterial species are closely related, phenotypically as well as phylogenetically, sharing the same 16s rrna gene sequence. both species contain specific protein toxins from the family of pore-forming rtx toxins, however, the two species differ in their rtx toxin profiles. haemolyti ...200312488079
phylogenetic relationship of equine actinobacillus species and distribution of rtx toxin genes among clusters.equine actinobacillus species were analysed phylogenetically by 16s rrna gene (rrs) sequencing focusing on the species actinobacillus equuli, which has recently been subdivided into the non-haemolytic a. equuli subsp. equuli and the haemolytic a. equuli subsp. haemolyticus. in parallel we determined the profile for rtx toxin genes of the sample of strains by pcr testing for the presence of the a. equuli haemolysin gene aqx, and the toxin genes apxi, apxii, apxiii and apxiv, which are known in po ...200312791244
delineation of the genus actinobacillus by comparison of partial infb sequences.a 426 bp fragment of infb, a housekeeping gene that encodes translation initiation factor 2, was sequenced from 59 clinical isolates and type strains of actinobacillus species and sequences were compared. partial sequences of 16s rrna genes were also obtained. by comparing infb sequences, actinobacillus pleuropneumoniae, actinobacillus equuli, actinobacillus suis, actinobacillus ureae, actinobacillus arthritidis, actinobacillus hominis and two unnamed genomospecies showed more than 85 % similari ...200415143001
actinobacillus suis infection of horses.nineteen isolates of actinobacillus suis were recovered from horses during the period october 1978-december 1980. animals varied in age from a full term foetus to 12 years. one isolate was obtained from the nose of an apparently healthy horse, the remainder were obtained from still-born foetuses (2), foals dying within a week of birth (5), older animals with respiratory (6) or genital infections (3) or abscesses in the jaw (1). one isolate was obtained from the lung of a 2-week-old foal which ha ...198216030875
actinobacillus suis infection in pigs in australia.this is the first report of the isolation of actinobacillus suis in association with significant disease among preweaned pigs in australia. sudden deaths occurred in preweaned piglets at one facility and enlarged joints, particularly the stifles and tarsi, occurred at another. isolates with the biochemical phenotype and apx genotype profile of a suis were cultured from affected piglets. both facilities were of high health status and one had undergone a recent depopulation, disinfection and sow r ...200010904814
aroa gene pcr-rflp diversity patterns in haemophilus parasuis and actinobacillus species.the haemophilus parasuis aroa gene encodes 5-enolpyruvylshikimate-3-phosphate synthase and participates in the aromatic amino acids and the folic acid universal metabolic pathway of bacteria. the application of aroa-based pcr-rflp methodology yields a significant degree of diversity in h. parasuis and actinobacillus species. pcr amplification of the aroa gene rendered a 1,067-bp fragment in all 15 h. parasuis serovars, and also in actinobacillus pleuropneumoniae serotypes 1-12, actinobacillus li ...200615936788
identification of actinobacillus suis genes essential for the colonization of the upper respiratory tract of swine.actinobacillus suis has emerged as an important opportunistic pathogen of high-health-status swine. a colonization challenge method was developed, and using pcr-based signature-tagged transposon mutagenesis, 13 genes belonging to 9 different functional classes were identified that were necessary for a. suis colonization of the upper respiratory tract of swine.200516177387
metalloproteases secreted by actinobacillus suis.actinobacillus suis secretes metalloproteases into its medium. these secreted proteins, when concentrated by precipitation with 70% (nh4)2so4 or methanol, displayed proteolytic activity at >200 kda molecular mass bands in 10% polyacrylamide gels copolymerized with bovine casein (1%). they showed activity in a broad ph range (from ph 5 to ph 10) and were inhibited by 20 mm edta or egta, but could be reactivated by calcium. they were found heat stable at 40 degrees c, 50 degrees c, 60 degrees c, a ...200415297931
porcine mannan-binding lectin a binds to actinobacillus suis and haemophilus parasuis.various collagenous lectins involved in innate immunity bind to surface oligosaccharides of bacteria and other microorganisms. we have been characterizing porcine plasma lectins that bind in a carbohydrate-dependent manner to surfaces of important bacterial pig pathogens including actinobacillus suis (as), a. pleuropneumoniae (app), and haemophilus parasuis (hp). a plasma protein with 32kda subunits (pi 5.4 and 5.75) bound most isolates of hp, as, and some app. partial amino acid sequences of th ...200616480769
the structural basis for the serospecificity of actinobacillus suis serogroup o:2.actinobacillus suis is an important bacterial pathogen of healthly pigs. an o-antigen (lipopolysaccharide; lps) serotyping system is being developed to study the prevalence and distribution of representative isolates from both healthy and diseased pigs. in a previous study, we reported that a. suis serogroup o:1 strains express lps with a (1-->6)-beta-d-glucan o-antigen chain polysaccharide that is similar in structure to a key cell-wall component in yeasts, such as saccharomyces cerevisiae and ...200616609699
emended description of actinobacillus capsulatus arseculeratne 1962, 38al.the taxonomic position of actinobacillus capsulatus, a member of the family pasteurellaceae found in rabbits, hares and hamsters, has been challenged. 16s rrna gene (rrs) sequence data show the species to be heterogeneous. using a polyphasic approach, 23 strains that were identified previously as belonging, or closely related, to a. capsulatus were analysed. eighty characters were included in the phenotypic analysis. phylogenetic analysis was done based on rrs, rpob, infb and recn sequences. in ...200717329797
[diagnostic valve of determination of the activity of certain blood serum enzymes in different forms of myocardial infarct]. 20154468565
developmental-behavioral dysfunction in preschool children. descriptive analysis of a pediatric consultative model.a study of 79 preschool children referred to an interdisciplinary clinic because of behavioral problems and developmental concerns was done. historical data and information regarding present functioning were collected using standard questionnaires. in addition to medical, neurological, and psychiatric examinations, every child received a neurodevelopmental assessment. when compared with a matched cohort of children from the community, the clinic population had a higher incidence of developmental ...200092192
deficiency of eosinophil peroxidase detected by automated cytochemistry. 20113122494
minimal incision techniques for digital deformities.we have presented basic minimal incision approaches to the more common digital deformities. we feel that with proper evaluation of the deformity and the patient, minimal incision surgery techniques give excellent long-term results with several advantages. these advantages include: less discomfort due to less surgical dissection. less need for internal fixation by careful use of compression dressings in the immediate postoperative period along with early ambulation. less disability. compression d ...20102937517
ontario. actinobacillus suis septicemia mimicking erysipelas in sows. 198817423089
actinobacillus suis infection in pigs in southwestern ontario.actinobacillus suis was isolated from tissues of 39 pigs, 2 porcine lungs, and 1 uterine swab submitted for diagnostic evaluation from 24 farms in southwestern ontario between 1985 and 1988. these isolates represented a gradually increasing incidence of herd outbreaks caused by a. suis in southwestern ontario. the outbreaks were typified by sudden death in suckling or recently weaned pigs; 87% of the affected pigs examined at the laboratory were between two and 28 days old. petechial to ecchymot ...199017423607
letter: diet and exercise in coronary heart-disease. 20144136082
serologic profile of a cohort of pigs and antibody response to an autogenous vaccine for actinobacillus suis.actinobacillus suis is a commensal opportunistic pathogen in swine. however, in recent years, an increasing prevalence of clinical signs associated with a. suis has been observed in high health status herds in north america. the objectives of the study were to assess the kinetics of antibodies to a. suis in pigs from a herd showing clinical signs of a. suis infection and, to evaluate the antibody response in gilts following vaccination with an autogenous vaccine. an enzyme-linked immunosorbent a ...200111361153
studies on the production of quorum-sensing signal molecules in mannheimia haemolytica a1 and other pasteurellaceae species.the bioluminescence assay system using vibrio harveyi reporter strains were used to examine quorum-sensing autoinducer (ai) activity from mannheimia haemolytica a1 cell-free culture supernatant. we showed that m. haemolytica a1 cell-free culture supernatant contains molecules that can stimulate the quorum-sensing system that regulates the expression of the luciferase operon in v. harveyi. specifically, m. haemolytica a1 can stimulate only the quorum system 2 but not system 1, suggesting that the ...200211786252
[clinical significance of lipid metabolic indices in diabetes mellitus in children]. 199898917
traumatic shock monitored by doppler ultrasonic flowmeter. case report. 1998124813
prevalence of organisms described as actinobacillus suis or haemolytic actinobacillus equuli in the oral cavity of horses. comparative investigations of strains obtained and porcine strains of a. suis sensu stricto.evidence was obtained to indicate that equine strains of organisms previously described as actinobacillus suis or hemolytic variants of actinobacillus equuli might constitute a separate group of organisms provisionally designated taxon 11. four biovars were noticed within taxon 11. selected dna:dna hybridizations support the classification of the mannitol positive biovar 2 of taxon 11 distinct from porcine a. suis. the final taxonomical position of taxon 11, however, has to await more detailed g ...19846532108
[lipid metabolism disorders: diagnosis and treatment. 4: drug therapy and dietary recommendations. national cholesterin-initiative]. 20061855762
enhanced resistance to bacterial infection in protegrin-1 transgenic mice.antibiotic-resistant bacteria have become a public health concern. it was suggested that one source of resistant pathogens may be food-producing animals. alternative approaches are therefore needed to enhance the resistance of farm animals to bacterial infection. protegrin-1 (pg-1) is a neutrophil-derived antimicrobial peptide that possesses activity against a wide range of bacteria and enveloped viruses. here we report on the production of transgenic mice that ectopically expressed pg-1 and com ...200818316527
prevalence of actinobacillus pleuropneumoniae, actinobacillus suis, haemophilus parasuis, pasteurella multocida, and streptococcus suis in representative ontario swine herds.tonsillar and nasal swabs were collected from weanling pigs in 50 representative ontario swine herds and tested for the presence of 5 important bacterial upper respiratory tract pathogens. all but 1 herd (2%) tested positive for streptococcus suis by polymerase chain reaction (pcr); 48% of herds were s. suis serovar 2, 1/2 positive. in all but 2 herds there was evidence of haemophilus parasuis infection. in contrast, toxigenic strains of pasteurella multocida were detected by a p. multocida--enz ...200818505187
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