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haemophilus somnus complex: antigenicity and specificity of fractions of haemophilus somnus.five haemophilus somnus type 8025 preparations (whole cell, sonicate, crude polysaccharide, purified polysaccharide, and protein) were produced for studies of their antigenicity in rabbits. bacterial agglutination and passive hemagglutination tests were used to assess the level of antibody produced in rabbits inoculated with the different antigenic preparations. cross-reactions were seen between the antiserums against the h sumnus 8025 antigens and a variety of related and unrelated bovine patho ...197550755
abscesses of the frontal lobe of the brain secondary to covert dental sepsis.the bacterial species found in pus aspirated from brain abscesses in two patients were typical of those found in dental sepsis. subsequently apical-root abscesses were demonstrated in the upper jaws of both patients. this evidence strongly suggests that these cerebral abscesses were secondary to dental sepsis which could have spread from the teeth to the frontal lobes by several possible antaomical pathways.197879867
significance of noncapsular antigens in protection against experimental haemophilus influenzae type b disease: cross-reactivity.a bacterial strain, tentatively identified as an actinobacillus species, was found to asymptomatically colonize the pharynx of some rats and to bear cell wall antigens which cross-react with noncapsular antigens of haemophilus influenzae type b (hib). the cross-reacting antigens appeared to be a heterogeneous mixture with varying molecular size and charge. the antigenic moieties are probably carbohydrate in nature. antisera raised with this strain had both immunochemical and biological (bacteric ...197880382
actinobacillus seminis infection in sheep in the republic of south africa. iii. growth and cultural characteristics of a. seminis.bacteriological tests were done on a large number of different strains of actinobacillus seminis and also, repeatedly, on the same culture or on different cultures taken periodically from the same donor animal. these tests were also applied to strains of a. seminis representing different serological types, which in turn were compared with strains of brucella ovis. the tests as applied proved that a. seminis strains have defined, morphological, staining, cultural and biochemical properties, altho ...197995214
serologic study of pasteurella aerogenes sp n.the antigenic attributes of pasteurella aerogenes sp n were serologically compared with species of the genera actinobacillus and pasteurella. examination included the tube-agglutination and double-immunodiffusion techniques. the results indicated the possibility of serologically different strains of p aerogenes. antisera prepared from strains of p aerogenes also reacted well with antigens prepared from yersinia pseudotuberculosis (p pseudotuberculosis) and p pneumotropica.1979112888
extraction and partial characterization of a leukotoxin from a plaque-derived gram-negative microorganism.the plaque-derived gram-negative microorganism y4 identified as a member of the genus actinobacillus, was tested for a soluble cytotoxic factor(s). sonication or incubation of viable y4 microorganisms in distilled water or normal human serum resulted in liberation of a soluble material which was cytotoxic in vitro for human polymorphonuclear leukocytes (pmns). the y4 soluble sonic extract was also cytotoxic to human peripheral blood monocytes. however, human lymphocytes, platelets, and fibroblas ...1979113347
lipoquinones of some spore-forming rods, lactic-acid bacteria and actinomycetes.the respiratory quinones of 73 strains of gram-positive bacteria including spore-forming rods, lactic-acid bacteria and actinomyctes were examined. menaquinones with seven isoprenoid units (mk-7) were the main quinone type found in representatives of the genus bacillus and in sporolactobacillus inulinus. however, a strain of b. thuringiensis produced mk-8 in addition to mk-7, and strains of b. lentus and b. pantothenticus appeared to produce mk-9 and mk-8, respectively, with no mk-7. in the clos ...1979119033
[comparative characteristics of the ultrastructure of the causative agents of glanders and melioidosis].the authors examined the ultrastructure of the causative agents of glanders and melio idosis. it was revealed that the structure of their cell wall and of the cytoplasmic membrane was characteristic of gram negative bacteria. the cytoplasm of both types of the causative agents showed the presence of ribosomes, membrane structure, nucleoid, and also osmiophilic and osmiophobic inclusions.1975174344
phosphatase activity of aerobic and facultative anaerobic bacteria.1115 strains of aerobic and facultatively anaerobic bacteria were tested for phosphatase activity by a conventional plate method and a microtest. the microtest was devised to allow results to be read after 4 h cultivation. phosphatase activity was found in wide range of species and strains. besides staphylococci, where the test for phosphatase is successfully used, it may be applied as one of the valuable tests for the differentiation of the following species: bacillus cereus, b. licheniformis, ...1978216188
isolation of actinobacillus actinomycetemcomitans and haemophilus aphrophilus at auckland hospital.actinobacillus actinomycetemcomitans and haemophilus aphrophilus were each isolated three times at auckland hospital, over a one year period. relevant clinical particulars of the major cases are given, and problems of isolation, identity and treatment discussed.1979289008
a study of the bacteria associated with advancing periodontitis in man.samples of apical plaque were taken by means of an anaerobic gas-flushed syringe from 21 sites in eight patients. the samples were anaerobically dispersed, diluted and plated and incubated in an atmosphere of 80% n2, 10% h2 and 10% co2 for 7-21 days. all colonies on plates containing 20-50 isolates were picked, repeatedly restreaked, characterized and identified where possible by a probabilistic computer identification program. the sites were divided into four groups on the basis of clinical fea ...1979294457
prosthetic valve endocarditis by opportunistic pathogens.the incidence of endocarditis produced by the so-called "opportunists" as a complication of prosthetic valve surgery is progressively increasing in frequency and gradually transforming the clinical picture habitually associated with this disease. we report six cases of endocarditis produced by opportunistic microorganisms (two cases by candida, and the remaining by serratia, actinobacillus, acinetobacter calcoaceticus, and bacteroides fragilis, and by corynebacterium diphtheriae) in four male an ...1977319774
[differentiation of brucella by acrylamide-gel electrophoresis (author's transl)].the strains of brucella (tab. 1) were grown on tryptose-blood-agar at 37 degrees c, and the strains of bordetella, pasteurella and actinobacillus on blood-agar. after 24 or 48 h they were harvested and extracted in phenol-acetic acid-water solution (4:2:1) (1 ml/50 mg bacterial wet weight) at 4 degrees c over 48 h. after centrifugation (8000 x g, 1 h) 2 volumes of the supernatant were mixed with 1 volume of a 40% sucrose solution in 35% acetic acid. different volumes (0.20, 0.15 or 0.10 ml) of t ...1977331758
[differentiation of "pasteurella" and "actinobacillus" from enterobacteriaceae" by use of the o/129 vibriostatic agent (author's transl)].out of 134 pasteurella and actinobacillus strains studied, 132 are found to be susceptible to the o/129 vibriostatic agent with growth inhibition diameter ranging from 10 to 42 mm (86% in the range 25 to 32 mm); 46 reference strains of enterobacteriaceae including yersinia are resistant to the o/129 vibriostatic agent. this test can quickly differentiate between typical or atypical enterobacteriaceae and the genus pasteurella or actinobacillus.1979389129
echocardiographic appearance of aortic valve vegetations in bacterial endocarditis due to actinobacillus actinomycetemcomitans.this report describes the echocardiographic features seen in a case of bacterial endocarditis due to actinobacillus actinomycetemcomitans. the echocardiogram demonstrated large, bulky clusters of echoes filling the aortic root in diastole which extended to the subvalvular and supravalvular areas. the echocardiographic features mimicked those described in cases of candida endocarditis. the morphologic appearance of vegetations at surgery correlated with the echocardiographic findings.1977402396
vertebral osteomyelitis due to actinobacillus actinomycetemcomitans. 1979430752
serological variants of actinobacillus lignieresii in slaughtered cattle. 1979430936
infective endocarditis caused by slow-growing, fastidious, gram-negative bacteria.in a review of endocarditis caused by fastidious, slow-growing gram-negative rods, similarities in the spectrum of disease overshadow differences among cases grouped by specific organisms. cardiobacterium hominis, actinobacillus actinomycetemcomitans and haemophilus species usually seed previously damaged cardiac valves presumably during bacteremia from an upper respiratory site. the clinical presentation resembles that of streptococcus viridans endocarditis and is usually subacute or chronic. d ...1979431400
bacterial isolates associated with epididymitis in rams from idaho and eastern oregon flocks.testicles from 47 rams with palpable lesions of epididymitis were subjected to bacterial culture. of the testicles examined by culture technique, 49% were positive for bacterial growth. the 2 most frequent isolates were actinobacillus actinomycetemcomitans (23.4% of the testicular pairs) and brucella ovis (14.9% of the testicular pairs). heretofore a actinomycetem-comitans has not been reported as a cause of epididymitis in rams.1979453669
determination of serovar of autoagglutinating strain of actinobacillus lignieresii by gel precipitation test. 1979459948
endocarditis caused by actinobacillus actinomycetemcomitans.a case of endocarditis caused by actinobacillus actinomycetemcomitans is described and 18 cases are reviewed. clinical features of endocarditis caused by a actinomycetemcomitans and antibiotic susceptibility are reviewed. because of the relative resistance of a actinomycetemcomitans to penicillin and ampicillin, high doses of either penicillin or ampicillin should be combined with streptomycin or gentamicin for four to six weeks.1979472858
actinobacillus septicemia in a black swan (cygnus atratus).a species of actinobacillus was isolated in pure culture from the carcass of a captive australian black swan (cygnus atratus) dying of acute septicemia. the gross and microscopic findings are described and the significance of the isolate is discussed.1979501838
medium for selective isolation of fusobacterium nucleatum from human periodontal pockets.a selective medium, cve agar, was developed for the isolation of fusobacterium nucleatum from subgingival plaque of periodontally diseased patients. the medium contained 1.0% trypticase (bbl microbiology systems), 0.5% yeast extract, 0.5% nacl, 0.2% glucose, 0.02% l-tryptophan, 1.5% agar, and 5% defibrinated whole sheep blood. erythromycin and crystal violet were added as the selective inhibitory agents at concentrations of 4 and 5 micrograms/ml, respectively. the medium permitted almost total r ...1979521483
isolation of actinobacillus suis from a colt. 1978630374
disseminated actinomycosis.a case of pulmonary actinomycosis with dissemination to skin, subcutaneous tissue, and bone is described. the cutaneous lesions were characterized by tender swellings and fluctuant nodules and were the presenting manifestation of the disease. typical sulfur granules were present in the skin and pleural biopsies. actinomyces israelii and its synergistic bacteria actinobacillus actinomycetemcomitans were cultured from skin and pleura. clinical improvement, with resolution of lung and disseminated ...1978635629
haemophilus aphrophilus cholecystitis.cultures from the gallbladder and blood of a 60-year-old man with acute cholecystitis grew haemophilus aphrophilus. this organism, an unusual isolate in clinical specimens, is most frequently seen in patients with either endocarditis or brain abscesses. haemophilus aphrophilus may be distinguished from eikenella corrodens and actinobacillus actinomycetemcomitans on the basis of colonial morphology and the biochemical tests for oxidase and catalase production and fermentation of lactose, sucrose, ...1978637050
actinobacillus actinomycetemcomitans endocarditis.two patients had infective endocarditis due to actinobacillus actinomycetemcomitans. one, a 52-year-old woman with a prosthetic aortic valve, was successfully treated with carbenicillin and gentamicin. the other, a 47-year old man with calcific aortic valve disease, required emergency valvectomy and prosthetic valve replacement and responded to a combination of penicillin and gentamicin.1978647545
mycetoma-like infection caused by previously undescribed bacterium.a 56-year-old man had a verrucous plaque with draining abscesses on the dorsum of hir foot for four years. examination of cultures revealed a gram-negative bacterium that, to our knowledge, has not been described in the literature. the infection was cured by treatment with co-trimoxazole (trimethoprim and sulfamethoxazole) and topical 10% lactic acid in hydrophylic emulsion base.1978677919
infection with actinobacillus suis in pigs. 1978696543
case report. cardiobacterium hominis: review of prior infections and report of endocarditis on a fascia lata prosthetic heart valve.the clinical spectrum of disease caused by cardiobacterium hominis, a small, gram-negative coccobacillus, is little known to physicians. from analysis of a new case of c hominis endocarditis on a fascia lata aortic valve, and from review of all previous reports of infection due to this microorganism, a remarkably consistent clinical picture emerged. c hominis only causes endocarditis, affects middle-aged individuals with structurally abnormal, left-sided cardiac valves, and follows a subacute co ...1978727215
testicular pathology of merino rams.the scrotal contents of 2,281 merino rams of wide age range examined at 3 abattoirs in perth, western australia, showed a 40% prevalence of rams with 1 or more gross lesions. the percentage prevalence of gross lesions found on autopsy were: adhesions 21, testicular atrophy/hypoplasia 14, testicular calcification 13, congenital cyst of the epididymis 6, cryptorchidism 4, chronic epididymitis/scrotal abscesses 2, varicocoele 2, testicular aplasia less than 1, seminoma less than 1. the prevalence o ...1978743044
serological comparison between histophilus ovis, actinobacillus seminis and brucella ovis.the serological relationships between 4 strains of histophilus ovis, the neotype strain of actinobacillus seminis and brucella ovis were examined using a cross-absorption complement-fixation technique. it was found that the 4 strains of h. ovis were serologically homologous and that an incomplete relationship existed between these organisms and a. seminis. anteriserums prepared against one strain of h. ovis and the strain of a. seminis gave a weak, apparently non-specific cross-reaction with br. ...1978743054
a taxonomic study of the genus haemophilus, with the proposal of a new species.a collection of 426 haemophilus strains isolated from people with infectious diseases and from the normal flora of mucous membranes in humans and various animal species was studied in an attempt to revise and improve the taxonomy of the genus haemophilus. the examinations included the determination of a number of biochemical and physiological properties, of which several had not previously been applied to the taxonomy of haemophili. the resulting data reavealed many hitherto unrecognized charact ...1976772168
[pathogenic role of various pleomorphic gram-negative bacteria]. 1976796897
butandioldehydrogenase in eikenella corrodens and related bacteria.the butandioldehydrogenase was examined in 153 strains of eikenella, moraxella, acinetobacter, agrobacterium, haemophilus, actinobacillus, pasteurella, cardiobacterium and "tm-1" of hollis et al. this enzyme has been proved to be useful in the differentiation of this group of bacteria.1976797181
initial colonization of teeth in monkeys as related to diet.the initial phases of plaque development on nonretentive tooth surfaces were studied bacteriologically in macaca irus monkeys fed by stomach tube and provided with various oral supplements. except for the oral implantation of streptococcus mutans in some of the animals, the oral flora was not changed prior to the studies. dental plaque was allowed to develop on initially cleaned tooth surfaces for 3 to 5 h. plaque samples were collected and cultured on a number of selective and nonselective agar ...1976825462
subacute bacterial endocarditis due to actinobacillus actinomycetemcomitans. report of a case with a review of the literature.a case of subacute bacterial endocarditis due to actinobacillus actinomycetemcomitans is reported. the patient was successfully treated first by a combination of gentamicin and ampicillin and then, because of severe allergic reactions, ampicillin was replaced by co-trimoxazole; symptoms did not recur and blood cultures remained sterile. a synoptic table is presented of 19 reported cases of infection caused by a. actinomycetemcomitans not connected with actinomycosis, with particular regard to th ...1977915011
a comparison of various haemophilus somnus strains.sixty-eight haemophilus somnus strains isolated from the bovine in canada and the u.s.a. were compared. in media enriched with 5% ovine serum, 5% bovine serum and 10% yeast extract, h. somnus fermented glucose, levulose, maltose, mannitol, mannose, sorbitol, trehalose and xylose, but failed to ferment arabinose, dulcitol, galactose, inositol, lactose, raffinose, rhamnose, salicin and sucrose. the organisms acidified litmus milk, produced cytochrome oxidase, indole and hydrogen sulfide (h(2)s) an ...1977922555
an outbreak of diphtheria in boergoat kids.during an outbreak of diphtheria or ulcerative stomatitis in young boergoat kids fusobacterium necrophorum was consistently isolated under anaerobic cultural conditions from material taken from the edges of the ulcerative lesions from all of the cases examined. corynebacterium pyogenes, staphylococcus aureus, actinobacillus lignieresi, moraxella spp. and lactobacillus spp. were also isolated anaerobically and/or aerobically. the typical symptoms and lesions were confirmed to the mouth, tongue an ...1976940095
porcine abortion caused by actinobacillus equuli.actinobacillus equuli was isolated from an aborted porcine fetus. fetal lesions of peracute embolic septicemia were similar to those seen in septicemic actinobacillosis of older swine and other species. this case represented an individual rather than a herd abortion problem.1976965291
actinobacillus suis infections in alberta swine, 1973--75: pathology and bacteriology. 1976974978
the isolation of actinobacillus equuli from equine abortion. 1976988818
foal septicemia (a case report). 19761049467
haemophili and related bacteria in the human oral cavity. 19751066093
cardiobacterium homonis endocarditis. characterization of the unusual organisms and review of the literature.a case of subacute bacterial endocarditis in which cardiobacterium hominis was isolated from the blood of a 55-year old woman who had rheumatic heart disease is reported. a survey of the literature revealed very few reports in which this organism has been implicated in human lesions. the colonies grew after 48 hours of incubation in a candle jar. they were small, convex, nonhemolytic, and oxidase-positive. the indole reaction was positive, the catalase and nitrate reactions were negative, and ac ...19751089355
[discovery of glanders bacillus]. 19751102281
rapid identification of gram-negative rods using a three-tube method combined with a dichotomic key.a three-tube method combined with a dichotomic key is presented which will identify virtually all enterobacteriaceae as well as a number of other frequently encountered gram-negative rods at a genus or species level within 20 hours following the primary isolation. the method is shown to be reliable and simple, saving processing time and material.19751106114
[antibiotic sensitivity of the causative agent of glanders].sensitivity of the glanders causative agent to 20 antibiotics representing different groups is described. the results of the in vitro studies showed that most of the antibiotics were not active against the glanders causative agent, except for polymyxin m, levomycetin and their combination. some relative activity was observed with neomycin. the "indicators" method used by the authors for determination of the m. mallei sensitivity to the antibiotics allowed the shortening of the time of the test b ...19751119794
endocarditis due to actinobacillus actinomycetemcomitans. 19751125627
cerebral actinomycotic granuloma.a rare case of primary cerebral actinomycosis is presented. pathologically the most important finding is represented by two types of lesions occurring in the same granulomatous mass without abscess formation. the possibility of an associated infection by actinomyces israelii and actinobacillus actinomycetemcomitans or lignieresi is discussed.19751235011
isolation of actinobacillus lignieresi from a epidural abscess in a horse with progressive paralysis. 19761245450
[diseases caused by some gram-negative pleomorphic microaerophilic organisms and absolute anaerobes]. 19761264846
actinobacillus suis in the horse. 19761265987
development and characterization of porphyromonas gingivalis-specific rat t-cell clones.porphyromonas gingivalis has been implicated as a major pathogen in periodontitis. to determine the role of t cells in the regulation of this disease, a method was developed for the generation and characterization of rat t-cell clones with antigen specificity to p. gingivalis whole cells. the clones studied so far demonstrated a t-helper (th) phenotype w3/13+, w3/25+, ox8- and ox22-. these t-cell clones proliferated in vitro in response to p. gingivalis, but not to other bacteria (prevotella int ...19921281976
rapid identification of actinobacillus actinomycetemcomitans based on analysis of 23s ribosomal rna.actinobacillus actinomycetemcomitans is a key microorganism in the pathogenesis of several different forms of periodontal diseases. identification of this bacterium from clinical specimens may often be complicated by the fact that the colony morphology on tsbv selective medium closely resembles that of haemophilus aphrophilus and a key differentiating characteristic, catalase reaction, may be variable. recent genetic studies have shown that the 23s ribosomal rna molecule is split into two smalle ...19921284398
antibacterial activity of dental implant metals.this study was conducted to investigate the specific in vitro antibacterial effect of seven dental implant metals on oral bacteria which have often been identified in failing implants. the metals chosen for evaluation were titanium, chromium, cobalt, aluminum, iron, gold, and vanadium. these metals were selected because they are contained in many commonly used dental implants. the bacteria selected for this study included isolates of porphyromonas endodontalis, porphyromonas gingivalis, prevotel ...19921288798
reactive antigens of the periodontopathic bacterium. actinobacillus actinomycetemcomitans. 19921295343
immunosuppressive effect induced by actinobacillus actinomycetemcomitans: effect on immunoglobulin production and lymphokine synthesis.the soluble sonicated extract (se) from actinobacillus actinomycetemcomitans inhibited primary t cell-dependent antibody responses in vivo. the production of igg and igm to sheep red blood cells (srbc) was depressed when mice were treated with high concentrations of se plus srbc. preinjection of se 3 days prior to srbc completely inhibited igg production. se plus srbc-primed mice showed markedly depressed cd4/cd8 ratios relative to phosphate-buffered saline plus srbc- or srbc-immunized mice. se- ...19921299800
evaluation of the e test for antimicrobial susceptibility testing of actinobacillus actinomycetemcomitans.the e test was compared with an agar dilution method for antimicrobial susceptibility testing of actinobacillus actinomycetemcomitans on mueller-hinton haemophilus test medium. eighty recent isolates of a. actinomycetemcomitans from 40 subjects and 3 reference strains were studied. excellent agreement was obtained between the e test and the agar dilution test for benzylpenicillin (98.8%), ampicillin (97.5%), cefaclor (100%), cefuroxime (100%), tetracycline hydrochloride (100%) and ciprofloxacin ...19921299801
actinobacillus actinomycetemcomitans in brazilian insulin-dependent individuals with diabetes mellitus.the prevalence of actinobacillus actinomycetemcomitans (aa) in subgingival plaque specimens from 26 insulin-dependent diabetes mellitus patients, 11-25 years of age, was determined between january 1987 and december 1989. one hundred and thirty subgingival plaque samples were collected with sterile periodontal curettes. the specimens were weighted, diluted, inoculated on trypticase-soy-serum-bacitracin-vancomycin agar medium (tsbv) and incubated under microacrophilic conditions. aa was isolated f ...19921303114
adjuvant properties of propionibacterium avidum kp-40 in vaccination against endemic viral and bacterial infections. ii. swine immunized with inactivated haemophilus pleuropneumoniae vaccine and experimentally infected with different virulent serotypes of h. pleuropneumoniae.forty 3-month old swine were treated with immunomodulating propionibacterium avidum kp-40 (pa) and/or vaccinated with a formalin-inactivated mixture of serotypes 1, 3, 5 and 9 of haemophilus pleuropneumoniae (pleurovac). three weeks after revaccination all animals were inoculated with viable single serotypes of haemophilus pleuropneumoniae. the igg antibodies induced by vaccination agglutinated all serotypes of haemophilus pleuropneumoniae, except for serotype 5. antibody titers were not influen ...19921303696
the effect of 3 mouthrinses on plaque and gingivitis development.the aim of this study was to evaluate the effect of 3 mouthrinses, listerine antiseptic (thymol), peridex (chlorhexidine), perimed (povidone iodine and hydrogen peroxide), and a placebo (water) on the development of dental plaque and gingivitis, when used as the only oral hygiene procedure for 14 days. 71 subjects were entered into a randomized, double-blind study. at the baseline examination, papillary bleeding score (pbs), and plaque index (pi) were registered, after which subjects received su ...19921310096
serum haptoglobin concentration in swine naturally or experimentally infected with actinobacillus pleuropneumoniae.serum haptoglobin (hp) concentrations were measured in swine that were naturally or experimentally infected with actinobacillus pleuropneumoniae. in swine from a specific-pathogen-free herd, mean serum concentration of hp (+/- sd) was 5.79 +/- 1.06 mg of cyanmethemoglobin-binding capacity (chbc)/dl. serum hp concentrations in paired samples were measured at 7-day intervals in 40 swine randomly selected from a conventional herd that was experiencing an acute episode of pneumonia and deaths caused ...19921293115
gingival crevicular fluid concentration and side effects of minocycline: a comparison of two dose regimens.the purpose of this study was to conduct a direct comparison of two dose regimens of minocycline to determine 1) whether they achieved crevicular fluid concentrations in a therapeutic range; and 2) the frequency of side effects. in a double-blind design, 30 patients divided into 2 groups were given either 100 mg minocycline or 200 mg minocycline per day for an 8-day period. the concentration of minocycline in the gingival clevicular fluid (gcf) at 8 days was 4.77 micrograms/ml for the 100 mg a d ...19921313100
microbiological and clinical results of metronidazole plus amoxicillin therapy in actinobacillus actinomycetemcomitans-associated periodontitis.we report on the microbiological and clinical effects of mechanical debridement in combination with metronidazole plus amoxicillin therapy in 118 patients with actinobacillus actinomycetemcomitans-associated periodontitis. patients were categorized into 3 groups: 28 had localized periodontitis; 50 had generalized periodontitis, and 40 had refractory periodontitis. after initial treatment and metronidazole plus amoxicillin therapy 114 of 118 (96.6%) patients had no detectable a. actinomycetemcomi ...19921313103
humoral antibody responses in periodontal disease.several forms of periodontal disease are considered to be infectious diseases with associated specific bacteria. this study examined the humoral antibody levels as assayed by elisa to actinobacillus actinomycetemcomitans, porphyromonas gingivalis, and prevotella intermedia in adult periodontitis (ap), localized juvenile periodontitis (ljp), rapidly progressive periodontitis (rpp), and in periodontally healthy subjects (hs). sixty-two of the 64 (96.9%) patients had significantly elevated antibody ...19921313106
evaluation of oral bacteria as risk indicators for periodontitis in older adults.the prevalence of people and sites with attachment loss, pocket depth, actinobacillus actinomycetemcomitans, prevotella intermedia, and porphyromonas gingivalis are described for a random sample of 366 black and 297 white community-dwelling adults, aged 65 or over, residing in five counties in north carolina. in addition, relationships between sites harboring these microorganisms and loss of attachment (la) and pocket depth (pd) are presented in a manner that considers the lack of independence o ...19921313107
pneumonia associated with salmonella choleraesuis infection in swine: 99 cases (1987-1990).salmonella choleraesuis was isolated in pure or mixed bacterial cultures from 153 swine necropsied between jan 1, 1987 and dec 31, 1990. pneumonia was seen in 99 of 109 swine from which this bacterium was isolated in the absence of other pathogenic bacteria. pneumonia was seen more frequently than hepatitis, splenomegaly, or colitis. pleuropneumonia that was grossly indistinguishable from the pleuropneumonia associated with actinobacillus pleuropneumoniae was seen in 29 of 99 swine from which s ...19921289347
microbiology of healthy and diseased periodontal sites in poorly controlled insulin dependent diabetics.a group of poorly-controlled insulin dependent diabetes mellitus (iddm) patients were examined in a cross-sectional design for total microbial levels, microbial incidence, and the percent levels of selected periodontal microorganisms. these organisms were selected on the basis of prior reports that associated them with either periodontal disease or health. one periodontally-healthy and one periodontally-diseased site were examined in each iddm patient. increased levels of the periodontal pathoge ...19921315389
hydrolysis of urea in the gastrointestinal tract of "monoxenic" rats: effect of immunization with strains of ureolytic bacteria.axenic rats, in whose feces urea is ordinarily excreted, were inoculated with ureolytic strains of lactobacillus or actinobacillus originally derived from the microflora of "holoxenic" rats. in these "monoxenic" animals, harboring one or another of the bacterial strains, fecal urea was hydrolyzed, with a more rapid onset of ureolysis in the case of actinobacillus as compared with lactobacillus. in vitro, a parallel difference between the two strains with regard to the onset of ureolysis was obse ...19761248879
the occurrence of periodontitis-related microorganisms in relation to titanium implants.the peri-implant space was examined for the presence of periodontitis-related microorganisms in 19 patients. all patients had been fitted with osseointegrated titanium implants which were clinically sound at the time of examination and all had been prescribed daily 0.2% chlorhexidine mouthrinse. in 17 patients, two contralateral fixtures in patients were selected, the remaining 2 patients had only one fixture, and associated mucosal clinical variables assessed for plaque accumulation, gingival i ...19921317427
the use of dna probes to examine the distribution of subgingival species in subjects with different levels of periodontal destruction.the present investigation examined the distribution of 14 subgingival species at a total of 2299 sites in 90 subjects with different levels of periodontal destruction. subgingival plaque samples taken from the mesial aspect of each tooth were anaerobically dispersed, diluted and plated on non selective media. after anaerobic incubation, colonies were lifted to nylon filters and specific species detected using digoxygenin-labeled whole chromosomal dna probes. the mean total viable count for all s ...19921318331
immunoglobulin-degrading enzymes in localized juvenile periodontitis.previous reports have indicated the association of periodontal diseases with elevated levels of serum immunoglobulin g (igg) antibodies to periodontally relevant bacteria. recent results from this laboratory suggest that enzymes proteolytic for immunoglobulins are important virulence factors of several periodontal bacteria. specifically, enzymes from porphyromonas (bacteroides) gingivalis culture supernatant fluid (sf) cleaved human igg (4 subclasses), iga1 and iga2, igm, igd and ige. proteolyti ...19921318974
humoral igg antibodies to oral microbiota in a population at risk for root-surface caries.mutans streptococci have been strongly implicated in the initiation of dental caries on coronal surfaces. their role in development of root-surface caries is less clear. the etiologic agents of both types of dental caries are likely to elicit systemic immune responses. the objective of the present study, therefore, was to study the association of clinical variables of disease with humoral igg antibodies to nine oral micro-organisms in 314 adult subjects, aged 45-65 years, who were at risk for ro ...19921321176
subgingival temperature (iii). relation to microbial counts.the present investigation examined the relationship of selected bacterial species and subgingival temperature. 35 subjects were measured at 6 sites per tooth for clinical parameters and subgingival temperature. measurements were repeated for 21 subjects at 2 month intervals providing a total of 66 subjects visits. at each visit, subgingival plaque samples were taken from the mesial aspect of each tooth and anaerobically dispersed, diluted and plated on non-selective media. after anaerobic incuba ...19921321846
e-test: a new technique for antimicrobial susceptibility testing for periodontal microorganisms.the purpose of the study was to validate the epsilometer test (e-test) method for antimicrobial susceptibility testing of selected periodontopathic microorganisms using the agar dilution method as a standard. the e-test has been developed to provide a direct quantification of antimicrobial susceptibility of microorganisms. the device consists of a predefined, continuous, and exponential gradient of antibiotic concentrations immobilized along a rectangular plastic test strip. after 48 hours incub ...19921324301
development of a rapid latex agglutination test for periodontal pathogens.the studies reported here describe the development, characterization, and initial application of latex agglutination assays for periodontal pathogens. latex reagents were prepared by sensitization of latex microspheres with rabbit igg antibodies to either actinobacillus actinomycetemcomitans, porphyromonas gingivalis, or prevotella intermedia. the protein concentration utilized for sensitization and microsphere size were optimized. one reagent was prepared to a. actinomycetemcomitans and a secon ...19921324302
synergistic effect of pyrophosphate and sodium dodecyl sulfate on periodontal pathogens.our previous studies have shown that pyrophosphate (ppi), the anticalculus component of tartar-control dentifrices, inhibits the growth of organisms associated with coronal and root surface caries. the purposes of this investigation were to: 1) determine if periodontal pathogens are similarly susceptible to the growth-inhibitory properties of ppi; and 2) determine if combinations of pyrophosphate-sodium dodecyl sulfate (ppi-sds) inhibit growth synergistically. porphyromonas gingivalis, actinobac ...19921324304
a preliminary study on the effects of the nd:yag laser on root surfaces and subgingival microflora in vivo.the purpose of this study was to examine the effects of root preparation using the pulsed nd:yag laser, either alone or in combination with manual instrumentation. study specimens consisting of 18 teeth with associated periodontal pockets from 8 different patients were treated as follows: 5 specimens were root planed with curets followed by laser exposure for 3 minutes using energy settings of 3.0 w at 20 pps; 2 specimens were root planed and then laser treated for 3 minutes using settings of 2. ...19921324305
anti-proliferative and cytotoxic activity of surface-associated material from periodontopathogenic bacteria.the easily solubilized surface-associated material from three bacterial species associated with periodontal diseases, actinobacillus actinomycetemcomitans, porphyromonas gingivalis and eikenella corrodens, produced dose-dependent inhibition of thymidine incorporation by human fibroblasts, the human monocytic cell line u937 and guinea pig epidermal cells. in contrast, lipopolysaccharides from a. actinomycetemcomitans and p. gingivalis were either inactive or substantially less active over the dos ...19921325147
supragingival cleaning 3 times a week. the microbiological effects in moderately deep pockets.it is generally believed that supragingival plaque control has little effect on the subgingival microflora of deep periodontal pockets. however, this may not be true for moderately deep pockets (4-5 mm), which may represent a pathological state between gingivitis and marginal periodontitis. in 6 patients with poor oral hygiene and severe gingival inflammation, 4 matched sites (1 in each quadrant), were chosen which demonstrated at least 20% spirochetes and 15% black-pigmented gram-negative bacil ...19921325484
microbial etiology of periodontal diseases. where are we? where are we going?many studies indicate a strong association of actinobacillus actinomycetemcomitans with localized juvenile periodontitis. species associated with adult periodontitis include bacteroides forsythus, porphyromonas gingivalis, prevotella intermedia, a. actinomycetemcomitans, and wolinella recta. capnocytophaga species may be important in pubertal gingivitis. an unnamed spirochete related to treponema pallidum has been identified in acute necrotizing ulcerative gingivitis lesions. species isolated fr ...19921325846
construction of mutants of actinobacillus actinomycetemcomitans defective in serotype b-specific polysaccharide antigen by insertion of transposon tn916.mutants of actinobacillus actinomycetemcomitans strain y4 defective in the capsular-like serotype b-specific polysaccharide antigen (spa) were constructed by inserting the transposon tn916. southern blot analysis suggested that the transposon was inserted into a variety of different sites on the chromosome. whole cells from two mutants (strains st1 and st2) lacked reactivity with a monoclonal antibody to spa of a. actinomycetemcomitans y4 (mab s5) in enzyme-linked immunosorbent assay, but those ...19921326593
neutrophil modulation by actinobacillus actinomycetemcomitans. ii. phagocytosis and development of respiratory burst.compromised neutrophil function has been found in a number of patients with localized juvenile periodontitis (ljp), although the pathogenic mechanism is unknown. since infection with actinobacillus actinomycetemcomitans is frequently found in patients with ljp, we have evaluated in vitro the effect of a bacterial extract of a. actinomycetemcomitans on the development of the respiratory burst by neutrophils. pre-incubation of neutrophils with bacterial extract increased h2o2 induced by fmlp and z ...19921328589
comparative study of subgingival microbiological sampling techniques.the presence of specific bacteria in subgingival plaque has been used as an indicator of active periodontal disease. the technique of subgingival sampling may conjecturally influence the identification and enumeration of microorganisms reported. in this study, paper point sampling and scaler sampling are compared. subgingival samples using both methods were taken from three surfaces in each of 12 patients at the following time points: at each of two appointments one week apart before treatment a ...19921328591
longitudinal monitoring for disease progression of localized juvenile periodontitis.the present study was performed to investigate the factors associated with disease progression in localized juvenile periodontitis (ljp) patients by longitudinal monitoring of microbiological changes. following a 9-month period, 9 ljp patients were divided into 2 groups based upon attachment loss, progressing and non-progressing. both groups received scaling, root planing, and modified widman flaps. clinical and microbiological data were obtained at baseline, following the observation period, an ...19921328592
in vitro activity of azithromycin compared with that of erythromycin against actinobacillus actinomycetemcomitans.the in vitro susceptibility of actinobacillus actinomycetemcomitans to azithromycin, a new macrolide antibiotic of a new class known as azalides, was compared with that of erythromycin by the agar dilution method on mueller-hinton haemophilus test medium. eighty-two a. actinomycetemcomitans strains, 79 recent clinical isolates obtained from 40 periodontally healthy or diseased subjects, and 3 type strains were included in the study. erythromycin showed poor in vitro activity against a. actinomyc ...19921329617
comparative in-vitro activity of azithromycin, macrolides (erythromycin, clarithromycin and spiramycin) and streptogramin rp 59500 against oral organisms.the in-vitro activities of azithromycin, clarithromycin, spiramycin and rp 59500 were compared with erythromycin against a wide range of oral organisms which have been implicated in oral infections and/or endocarditis (clindamycin was included for oral streptococci). all compounds tested showed good activity against many of these organisms, although some variation was observed with different species. clarithromycin was the most active of the antibiotics tested against gram-positive anaerobes, in ...19921331019
occurrence of periopathogens in smoker and non-smoker patients.the aim of the present study was to elucidate the interrelationship between cigarette smoking and the occurrence of periopathogens, i.e., actinobacillus actinomycetemcomitans, bacteroides gingivalis and bacteroides intermedius. the study was based on 145 patients with clinically severe periodontitis, 52 men (age range 32-73 years) and 93 women (age range 32-74 years). 83 patients were smokers (> or = 15 cigarettes/day) and 62 non-smokers. bacterial samples were collected from one site with a pro ...19921331203
comparative clinical and microbiological effects of topical subgingival application of metronidazole 25% dental gel and scaling in the treatment of adult periodontitis.the aim of the study was to compare the clinical and microbiological effects of topical application of a metronidazole gel and a single session of subgingival scaling in the treatment of adult periodontitis. an open, randomized controlled clinical study design was employed. each of 24 subjects received the 2 treatments simultaneously each in 2 randomly selected quadrants of the dentition. the metronidazole, 25% gel was applied subgingivally on days 0 and 7. scaling was carried out in one quadran ...19921332988
sensitization of oral bacteria in biofilms to killing by light from a low-power laser.biofilms of streptococcus sanguis, porphyromonas gingivalis, fusobacterium nucleatum and actinobacillus actinomycetemcomitans were prepared on the surfaces of agar plates and a number of compounds were screened for their ability to sensitize bacteria in these biofilms to killing by light from a 7.3 mw helium/neon (he/ne) laser. toluidine blue o and methylene blue enabled detectable killing of all four target organisms after exposure to he/ne light for 30 s. aluminium disulphonated phthalocyanine ...19921334649
expression of fc-gamma-riii and fibronectin in peripheral polymorphonuclear neutrophils with increased response to fc stimulation in patients with juvenile periodontitis.the nature of increased luminol-enhanced chemiluminescence (cl) in peripheral polymorphonuclear neutrophils (pmn) in juvenile periodontitis is of pathophysiological interest and may serve as a model for tissue damage caused by granulocytes. activation of pmns by opsonized staphylococcus aureus was compared with that of actinobacillus actinomycetemcomitans and bacteroides gingivalis, regarded as being more specific for juvenile periodontitis. the cl was higher in the pmns from the patients, indep ...19921335228
inhibition of bone dna and collagen production by surface-associated material from bacteria implicated in the pathology of periodontal disease.gentle extraction of oral bacteria implicated in the pathogenesis of periodontal disease, actinobacillus actinomycetemcomitans, porphyromonas gingivalis, or eikenella corrodens, with saline removes the extracellular components while leaving the bacteria intact. this readily-solubilized surface-associated material (sam) has been demonstrated to significantly inhibit dna and collagen synthesis by murine calvaria at concentrations as low as 10 ng/ml. dna and collagen synthesis in isolated calvarial ...19921335499
bacterial concentration fluorescence immunoassay (bcfia) for the detection of periodontopathogens in plaque.a bacterial concentration fluorescence immunoassay (bcfia) was developed to rapidly detect periodontopathic bacteria in human plaque samples. the bcfia utilized fluorescent-tagged monoclonal antibodies (mabs) directed against the lipopolysaccharide of selected gram-negative bacteria. microorganisms identified in plaque using the bcfia included actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, and fusobacterium nucleatum. the immunoassay procedure involved com ...19921336047
dna probe and enzyme analysis in periodontal diagnostics.recent research in periodontology has indicated that a finite number of bacterial species are associated with periodontal disease. this has generated the hypothesis that periodontal disease is essentially an infection due to one or more of the putative periodontopathogens; i.e., the specific plaque hypothesis (sph). this microbial specificity paradigm has already changed the way researchers and some clinicians view periodontal disease. the clinician must heed several cautions signs, however, if ...19921336048
characteristics and utilization of antibody measurements in clinical studies of periodontal disease.the detection and quantitation of immune responses to infections have long been used as a diagnostic tool in medical infections. recently, increasing evidence has supported that active, specific antibody responses to selected members of the subgingival microbiota are noted in periodontitis patients. this report describes the various specificities of this antibody as they relate to periodontitis classification and prognosis. the functional aspects of the serum antibody have come under increasing ...19921336049
antimicrobial effect of a dental varnish, in vitro.the effects of a polymer based antimicrobial releasing varnish cervitec were investigated against different gram-positive and gram-negative bacterial strains as well as a yeast using the agar diffusion inhibitory test (adt-test in vitro). as positive controls a 1% chlorhexidine gel and 1% aqueous solution of thymol and a placebo polymer varnish without active agents were employed. the test experimental varnish containing 1% chlorhexidine and 1% thymol showed antimicrobial activity against all gr ...19921336221
[the value of using serum specific antibody in differential diagnosis of periodontitis].in the present study, serum anti-bacteroides gingivalis (bg) and anti-actinobacillus actinomycetemcomitans (aa) antibody titers in rpp, ap patients and healthy subjects were determined by elisa. discriminant analysis for differential diagnosis of rpp, ap and healthy subjects was performed using clinical measurements (pd, al, pii, bi) and serum antibody levels as combined parameters. the results showed significantly different serum antibody levels among the three examined groups. the results also ...19921338038
an unusual epizootic of actinobacillosis in dairy heifers.actinobacillosis developed in a group of 52 holstein-friesian replacement heifers. they were confined to 4 pens in a barn. cervical abscesses were first observed by the owner in 1 heifer a month after the heifers were fed chopped haylage that was particularly dry, tough, and stemmy. during the weeks that followed, morbidity reached 73% in heifers given the feed. calves up to 4 months old were not given the haylage and did not develop the disease. standard bacteriologic methods were used to ident ...19751168181
antibacterial activity of some triclosan-containing toothpastes and their ingredients.the antibacterial activity of 4 triclosan-containing toothpastes was compared to a conventional fluoride dentifrice and triclosan and sodium lauryl sulphate (sls), both singly and in combination. a panel of 17 bacteria was tested by an agar dilution method. at concentrations typical of those found in toothpastes, triclosan and sls displayed approximately equal antibacterial activity. a paste containing triclosan and zinc citrate appeared more active than the other triclosan pastes which, in gene ...19921345321
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