| purification and some properties of two nadp+-specific isocitrate dehydrogenases from an obligately psychrophilic marine bacterium, vibrio sp., strain abe-1. | two isozymes of nadp+-specific isocitrate dehydrogenase [icdh; ec 1.1.1.42] were confirmed to be present in an obligately psychrophilic marine bacterium, vibrio sp., strain abe-1, on the basis of the temperature-activity curve and electrophoretic mobilities. these isozymes were separated and purified about 170-fold for isozyme i (specific activity at 40 degrees c, 24.3 units/mg protein) and about 180-fold for isozyme ii (specific activity at 20 degrees c, 59.2 units/mg protein), though the isozy ... | 1979 | 39069 |
| bacterial flora of the hemolymph of the blue crab, callinectes sapidus: numerical taxonomy. | bacteria isolated from the hemolymph of normal blue crabs were found to be predominantly vibrio sp., pseudomonas sp., acinetobacter sp., bacillus sp., flavobacterium sp., and coliforms. vibrio parahaemolyticus, a cosmopolitan facultative pathogen widely implicated in outbreaks of gastroenteritis related to the consumption of improperly processed seafood, was present in crabs collected between the months of may to november and was identified in up to 21% of the hemolymphs sampled. | 1975 | 1090258 |
| synthesis in vitro of very long chain fatty acids in vibrio sp. strain abe-1. | the activity of fatty acid synthetase (fas) from vibrio sp. strain abe-1 required the presence of acyl carrier protein and was completely inhibited by thiolactomycin, an inhibitor specific for a type ii fas. these observations indicate that this enzyme is a type ii fas. analysis by gas-liquid chromatography of the reaction products synthesized in vitro from [2-14c]malonyl-coa by the partially purified fas revealed, in addition to 16- and 18-carbon fatty acids which are normal constituents of thi ... | 1992 | 1510554 |
| expression of the b subunit of escherichia coli heat-labile enterotoxin in a marine vibrio and in a mutant that is pleiotropically defective in the secretion of extracellular proteins. | a marine vibrio (designated vibrio sp. 60) that is related to vibrio anguillarum was used as a host for a plasmid that encodes the non-toxic b subunit (etxb) of escherichia coli heat-labile enterotoxin. expression of etxb in vibrio sp. 60 resulted in the efficient and selective secretion of the b subunit into the extracellular growth medium. this indicated that vibrio sp. 60, which does not normally produce cholera-like enterotoxins, nonetheless possesses a secretory machinery that permits these ... | 1992 | 1588306 |
| cloning and nucleotide sequence of the vibrio proteolyticus aminopeptidase gene. | the gene encoding the vibrio proteolyticus aminopeptidase was cloned and sequenced and its amino acid sequence was deduced. the gene encodes a 54 kda protein, larger than the previously reported size of 30 kda for the purified aminopeptidase. sequence alignments revealed a 43-45% homology with two other vibrio sp. extracellular proteinases. | 1992 | 1627651 |
| enzymatic characterization of vibrionaceae strains isolated from environment and cold-blooded animals. | enzymatic profiles were determined by the api zym system for 15 strains of non 01 vibrio cholerae, 4 strains of v. metschnikovii, 9 strains of v. anguillarum, 6 strains of plesiomonas shigelloides and 115 strains motile aeromonas sp. all of the tested strains produced alkaline phosphatase, leucine aminopeptidase and did not possess alpha-fucosidase and alpha-mannosidase. some differences in enzymatic activities among the tested vibrionaceae strains were noted. the strains of non 01 v. cholerae, ... | 1991 | 1725094 |
| the cis/trans isomerization of the double bond of a fatty acid as a strategy for adaptation to changes in ambient temperature in the psychrophilic bacterium, vibrio sp. strain abe-1. | the major phospholipid, phosphatidylethanolamine (pe), of vibrio sp. strain abe-1 contains a unique trans-unsaturated fatty acid, 9-trans-hexadecenoic acid (16:1(9t], at the sn-2 position of the glycerol moiety. the major molecular species of pe that contain 16:1(9t) at the sn-2 position have either 9-cis-hexadecenoic acid (16:1(9c] or hexadecanoic acid (16:0) at the sn-1 position. the transition temperatures of the liquid-crystal to the gel phase of 16:1(9c)/16:1(9t)-pe and 16:0/16:1(9t)-pe wer ... | 1991 | 2054374 |
| purification and characterization of a novel beta-agarase from vibrio sp. ap-2. | beta-agarase was purified from the culture fluid of a porphyran-decomposing marine bacterium (strain ap-2) by ammonium sulfate precipitation, successive column chromatography and dnase and rnase treatment. the final enzyme preparation appeared to be homogeneous on polyacrylamide gel electrophoresis. the enzyme had a molecular mass of 20 kda, a ph optimum of 5.5, and was stable in the ph region 4.0-9.0 and at temperatures below 45 degrees c. the beta-agarase was a novel endo-type enzyme which hyd ... | 1990 | 2298219 |
| vibrio cincinnatiensis sp. nov., a new human pathogen. | a halophilic gram-negative rod was isolated from blood and cerebrospinal fluid collected from a 70-year-old male having no known contact with seafood or salt water. positive biochemical tests included oxidase, sensitivity to 0/129, o-nitrophenyl-beta-d-galactopyranoside, lysine decarboxylase and fermentation of glucose, salicin, n-inositol, sucrose, l-mannose, l-arabinose, and arbutin. negative tests included indole, ornithine decarboxylase, arginine dihydrolase fermentation of lactose, and prod ... | 1986 | 2422196 |
| isozymes of isocitrate dehydrogenase from an obligately psychrophilic bacterium, vibrio sp. strain abe-1: purification, and modulation of activities by growth conditions. | each of the two isozymes, which are different in thermostability and quaternary structure, of isocitrate dehydrogenase (nadp+) [idh: ec 1.1.1.42] was purified to an electrophoretically homogeneous state from an obligately psychrophilic marine bacterium, vibrio sp. strain abe-1. hydrophobic chromatography was an efficient procedure to separate the two isozymes from each other. the isoelectric points of isozyme i (idh-i; a dimer, mr 88,100) and isozyme ii (idh-ii; a monomer, mr 80,500) were found ... | 1987 | 3129416 |
| synthesis of membrane and periplasmic proteins during starvation of a marine vibrio sp. | changes in membrane and periplasmic protein profiles induced by starvation conditions in the marine vibrio sp. s14 were examined by one-dimensional gel electrophoresis. analysis by densitometry resolved at least six periplasmic proteins, nine outer membrane proteins, and four cytoplasmic membrane proteins induced at various times during 120 h of nutrient and energy starvation. eight of these were also synthesized by heat- and/or ethanol-shocked cells. pulse-labelling indicated that the starvatio ... | 1988 | 3221201 |
| characteristics of hg-resistant bacteria isolated from minamata bay sediment. | seventy-two strains of hg-resistant bacteria (pseudomonas) were isolated on agar plates containing 40 micrograms/ml of hgcl2 from minamata bay sediment, which was heavily polluted with mercury (45.8 micrograms/g). the minimal inhibitory concentrations (mics) of mercurial compounds were determined for the hg-resistant pseudomonads and 65 strains (pseudomonas sp., bacillus sp., vibrio sp., and corynebacterium sp.) isolated from sendai bay sediment (1 microgram/g of mercury) as control. the mics to ... | 1986 | 3709500 |
| extracellular nuclease produced by a marine bacterium. i. extracellular deoxyribonuclease formation by a marine vibrio sp. | deoxyribonuclease (dnase) activity was found in the culture fluids of numerous marine bacteria isolated from seawater. among these ogranisms, marine bacterium, vibrio sp., strain no. 2, showed the highest deoxyribonucleic acid-hydrolyzing activity. this organism requires salts of seawater for both growth and extracellular dnase formation. the dnase activity could not be detected in the synthetic seawater culture liquid lacking magnesium ion, and dnase activity decreased in a calcium-deficient me ... | 1976 | 974900 |
| growth characteristics of an obligately psychrophilic vibrio sp. | | 1977 | 879962 |
| experimental pathogenicity and mortality in ligated ileal loop studies of the newly reported halophilic lactose-positive vibrio sp. | laboratory animals were challenged subcutaneously, intraperitoneally, and intravenously with the halophilic lactose-positive vibrio. intraperitoneal inocula of 10(8) organisms proved to be rapidly lethal in mice, rats, and hamsters. the 50% lethal doese in icr strain mice was estimatd to be 8 x 10(5) live cells, injected intraperitoneally or subcutaneously. subtaneous inocula in mice resulted in severe local infections, characterized by gross edema, and for those animals surviving longer than 48 ... | 1978 | 669787 |
| novel transmissible factors in a non-o1 vibrio cholerae and a vibrio sp. | transmissible factors encoding production of lacunae (l factors) were demonstrated in a non-o1 vibrio cholerae and a vibrio sp. of recent environmental origin. lacunae were produced in lawns of non-o1 v. cholerae indicator strains under the same assay conditions as those where lacunae were produced by the well characterized p fertility plasmid of v. cholerae o1 and the v fertility factor found in a non-cholera vibrio strain. the origin of the lacunae produced by strains harbouring the v and l fa ... | 1986 | 3760815 |
| detection of a vibrio sp. by the bacteriophagous nematode pelodera chitwoodi. | | 1974 | 4418570 |
| temperature and hydrostatic pressure effects on gelatinase activity of a vibrio sp. and partially purified gelatinase. | | 1974 | 4619521 |
| postoperative wound infection associated with vibrio parahaemolyticus in a patient without exposure to seawater. | this report describes a case of wound infection associated with vibrio parahaemolyticus. the patient had ingested steamed crabs 7 days before admission for surgical treatment of intestinal obstruction due to colon carcinoma. the vibrio sp. was isolated from postoperative wound drainage as well as from stool. recovery was uneventful. | 1988 | 3384932 |
| mutagenesis by insertion of drug resistance transposon tn7 into a vibrio species. | a halotolerant, collagenolytic strain of vibrio sp. was conjugated with an escherichia coli strain carrying plasmid rp4. the plasmid was transferred to and maintained in the vibrio and could be subsequently transferred in matings to suitably marked stains of the same species. after conjugation with an e. coli carrying the cointegrate plasmid rp4::mu cts61::tn7, vibrio transconjugants were selected that carried tn7 inserted into the bacterial chromosome. a large proportion of these transconjugant ... | 1981 | 6270064 |
| occurrence of tetrodotoxin and anhydrotetrodotoxin in vibrio sp. isolated from the intestines of a xanthid crab, atergatis floridus. | vibrio sp. isolated from a xanthid crab, atergatis floridus, was cultured, and tetrodotoxin (ttx) and anhydrottx were indicated to be present in several fractions of the cell extract and the culture medium by reverse phase hplc. the presence of the c9-base in alkaline hydrolyzates of these fractions was confirmed by gc-ms and uv spectrometry. these results showed the production of ttx and anhydrottx in the vibrio sp., thus indicating one of the origins of ttx in nature. | 1986 | 3754255 |
| exclusion of clinically atypical or microbiologically mixed diarrhoeal episodes from outcome events in a field trial of oral cholera vaccines. | we investigated whether alternative clinical and microbiological criteria for outcome events affected estimates of vaccine efficacy in a randomized, double-blind field trial of b subunit-killed whole cell (bs-wc) and killed whole cell-only (wc) oral cholera vaccines among 62,285 rural bangladeshi participants. at one year of follow-up estimates of vaccine protective efficacy (pe = 60%, p less than 0.0001 for bs-wc; pe = 54%, p less than 0.0001 for wc) against all treated diarrhoeal episodes asso ... | 1989 | 2767860 |
| establishment of gene transfer systems for and construction of the genetic map of a marine vibrio strain. | two gene transfer systems were established for a marine bacterium, vibrio sp. strain 60. one was generalized transduction with a newly isolated bacteriophage, as3, and the other was conjugal gene transfer by the use of newly constructed transposon-facilitated recombination (tfr) donors. as3 transduced various chromosomal markers at frequencies of 10(-4) to 10(-6). tfr donors, which were constructed by introducing transposon tn10 into both plasmid rp4 and the chromosome, mediated the polarized tr ... | 1989 | 2539353 |
| metabolic and ultrastructural response to glucose of two eurytrophic bacteria isolated from seawater at different enriching concentrations. | two marine bacteria, an acinetobacter sp. (strain go1) and a vibrio sp. (strain g1), were isolated by extinction dilution and maintained in natural seawater supplemented with nitrogen, phosphorus, and glucose at 0.01 and 10 mg of glucose carbon per liter above ambient monosaccharide concentrations, respectively. after 3 days in unsupplemented natural seawater, growth in batch culture with glucose supplements was determined by changes in cell numbers and glucose concentration. the exponential gro ... | 1984 | 6696421 |
| isolation and characterization of mutants of a marine vibrio strain that are defective in production of extracellular proteins. | a marine vibrio strain, vibrio sp. strain 60, produces several extracellular proteins, including protease, amylase, dnase, and hemagglutinin. mutants of vibrio sp. strain 60 (epr mutants) pleiotropically defective in production of these extracellular proteins were isolated. they fell into two classes, a and b. in class a, no protease activity was detected in the cells either, whereas in class b, considerable protease activity was detected in the cells. gel electrophoretic analysis revealed that ... | 1988 | 2457013 |
| association of human enteric pathogenicity and mouse lung toxicity of escherichia coli. | mouse lung toxicity of 439 strains (431 escherichia coli, 1 shigella dysenteriae 1, 1 enterobacter cloacae, 5 vibrio sp., 1 klebsiella) was compared to other pathogenicity tests (mouse virulence, enterotoxicity, guinea pig eye test), to serogroup distribution, loss of virulence on storage, origin and haemolytic activity. mouse lethality was highest in serogroup o4 (p < 0.001), o18a,c (p < 0.001); serogroups o6, o20, o75, o115, o147 were next in order. e. coli serogroups o19, o26, o28a,b, o32, o5 ... | 1980 | 6998258 |
| differential expression in escherichia coli of the vibrio sp. strain abe-1 icdi and icdii genes encoding structurally different isocitrate dehydrogenase isozymes. | the expression of two structurally different isocitrate dehydrogenase isozymes of vibrio sp. strain abe-1 in escherichia coli was examined. at a low temperature (15 degrees c), a thermolabile and monomeric type isozyme (idh-ii), which is quite different in amino acid sequence from the e. coli isocitrate dehydrogenase, was expressed and conferred glutamate prototrophic ability on an e. coli mutant defective in isocitrate dehydrogenase. the ability of idh-ii to confer restoration of the e. coli mu ... | 1995 | 7536733 |
| a trans-unsaturated fatty acid in a psychrophilic bacterium, vibrio sp. strain abe-1. | a high level of a trans-unsaturated fatty acid was found in the phospholipids of a psychrophilic bacterium, vibrio sp. strain abe-1. this fatty acid was identified as 9-trans-hexadecenoic acid (c16:19t) by gas-liquid chromatography and infrared absorption spectrometry. c16:1(9)t accounted for less than 1% of the total fatty acids in cells grown at 5 degrees c and reached 12% of the total at 20 degrees c. we suggest that the increase in the level of the trans-unsaturated fatty acid is related to ... | 1990 | 2345157 |
| cloned aerolysin of aeromonas hydrophila is exported by a wild-type marine vibrio strain but remains periplasmic in pleiotropic export mutants. | with a wide host range vector, the structural gene aera for the hole-forming extracellular protein aerolysin of aeromonas hydrophila was cloned into the marine vibrio sp. strain 60 and into three pleiotropic export mutants (epr mutants). the parent strain and all of the mutants were able to express the protein with the aera promoter in the plasmid. the parent strain exported proaerolysin into the medium, while all of the mutants accumulated the protoxin in their periplasms. two of the mutants al ... | 1990 | 2294091 |
| marine natural products. xxxiv. trisindoline, a new antibiotic indole trimer, produced by a bacterium of vibrio sp. separated from the marine sponge hyrtios altum. | a new antibiotic indole trimer named trisindoline (1) was isolated, together with a known dioxopiperazine brevianamide f (2), from the culture of a bacterium of vibrio sp., which was separated from the okinawan marine sponge hyrtios altum. the structure of trisindoline (1) has been determined on the bases of physicochemical evidence and chemical synthesis. | 1994 | 7697760 |
| bacterial production of histamine in some tropical fish. | quantitative and qualitative distribution of histamine-forming bacteria associated with the fish rastrelliger kanagurta, sardinella longiceps, sillago sihama and liza subviridis, were investigated. these bacteria constituted a significant portion of the total bacterial population of fish and the values obtained in the present study were higher than those previously reported. the order of quantitative abundance of histamine-forming bacteria in the fish examined was: s. longiceps greater than r. k ... | 1990 | 2259288 |
| metabolism of linear alkylbenzenesulphonates by a vibrio sp. | | 1972 | 5076680 |
| starvation-induced modulations in binding protein-dependent glucose transport by the marine vibrio sp. s14. | the uptake kinetics of d-glucose were examined in the marine vibrio sp. s14 during a period of 168 h of complete energy and nutrient starvation. two glucose transport systems were distinguished in vibrio sp. s14: a low affinity system (km = 4.6 +/- 0.9 microm) at the onset of starvation, and a high affinity system (km = 0.55 +/- 0.15 microm) after 168 h of starvation. both systems had a narrow substrate specificity, and both were osmotic shock-sensitive. | 1990 | 2227357 |
| thymidine uptake, thymidine incorporation, and thymidine kinase activity in marine bacterium isolates. | one assumption made in bacterial production estimates from [3h]thymidine incorporation is that all heterotrophic bacteria can incorporate exogenous thymidine into dna. heterotrophic marine bacterium isolates from tampa bay, fla., chesapeake bay, md., and a coral surface microlayer were examined for thymidine uptake (transport), thymidine incorporation, the presence of thymidine kinase genes, and thymidine kinase enzyme activity. of the 41 isolates tested, 37 were capable of thymidine incorporati ... | 1990 | 2160223 |
| specific inhibition of the na(+)-driven flagellar motors of alkalophilic bacillus strains by the amiloride analog phenamil. | amiloride, a specific inhibitor for the na(+)-driven flagellar motors of alkalophilic bacillus strains, was found to cause growth inhibition; therefore, the use of amiloride for the isolation of motility mutants was difficult. on the other hand, phenamil, an amiloride analog, inhibited motor rotation without affecting cell growth. a concentration of 50 microm phenamil completely inhibited the motility of strain ra-1 but showed no effect on the membrane potential, the intracellular ph, or na(+)-c ... | 1990 | 2155207 |
| [conditions of the l-lysine decarboxylase biosynthesis in a culture of vibrio sp]. | the effect of l-lysine hcl concentrations, ph, temperature and cultivation conditions on the synthesis of intracellular lysine decarboxylase (ldc) by vibrio sp. was studied. the highest ldc activity was observed in a 4-hour culture grown at 30 degrees on a nutrient medium, ph 5.15 containing 0.9% l-lysine hcl as an inducer. under these conditions the ldc activity of the bacterium was 8.43 u/mg dry cells. | 1990 | 2112247 |
| [study, by the double diffusion in gel method, of the antigenic constituents of various strains of halophilic vibrio]. | the antigenic relationships among 18 strains of halophilic vibrio (5 v. para-haemolyticus, 5 v. alginolyticus, 3 v. anguillarum and 5 vibrio sp.) have been studied by a gel double diffusion test, either within the same species or with the others. some halophilic vibrio, named vibrio sp. and having a uncertain taxonomic position, have been found close related with v. parahaemolyticus or with v. alginolyticus, whereas others are, as well as v. angiullarum, less related to those species. | 1980 | 6161713 |
| proteolytic and lipolytic activities of micrococcus roseus (65), halomonas elongata (16) and vibrio sp. (168) isolated from danish bacon curing brines. | viable cells, cell free extracts and extracellular concentrates of micrococcus roseus (65), halomonas elongata (16) and vibrio sp. (168) isolated from danish bacon curing brines were examined for lipase, esterase, proteinase and aminopeptidase activities on natural and synthetic substrates. micrococcus roseus (65) produced one intracellular esterase with affinity for short chain esters, and two intracellular aminopeptidases with affinity for nonpolar amino acids and l-arginine, respectively. one ... | 1994 | 8074966 |
| diseases and injuries associated with mortality of hatchery reared baltic cod (gadus morhua l.) larvae. | a cod hatching plant was established in 1992 on the island of bornholm in the baltic sea in order to elucidate the possibilities for restocking of cod fry in this brackishwater system. the disease prevalence in 3 batches of hatchery-reared yolksac larvae from the baltic cod (gadus morhua l.) was monitored during the posthatch period. high prevalences of bacteriosis/mycosis, lordosis/scoliosis, injuries and protozoan endoparasitism were recorded. vibrio sp. and vibrio anguillarum serovar 04, 06, ... | 1993 | 8147291 |
| major diseases of striped bass and redfish. | diseases of striped bass, their hybrids, and redfish (red drum) are important constraints to the culture of these two species. since striped bass have been cultured for years the organisms that cause most diseases of these fish are well known, but very little specific disease information exists for redfish. however, it appears that the organisms that cause diseases of striped bass and redfish do not differ greatly from those of other fishes. the most significant viral disease is lymphocystis, bu ... | 1991 | 1926745 |
| the effect of temperature shifts on protein synthesis by the psychrophilic bacterium vibrio sp. strain ant-300. | in the psychrophilic bacterium vibrio sp. strain ant-300, the temperature-related characteristics of protein synthesis in cells grown at 0 degrees c differed from those of cells grown at 13 degrees c. cells grown at 0 degrees c and 13 degrees c transported amino acids at the same rates, dependent on the temperature at which rates were measured. the rates of protein synthesis in extracts of cells grown at 0 degrees c and at 13 degrees c differed, as a result of the changes in the properties of th ... | 1991 | 1856678 |
| changes in rates of synthesis of individual proteins in a psychrophilic bacterium after a shift in temperature. | in the psychrophilic bacterium vibrio sp. strain ant-300, which has the ability to grow efficiently between 13 and -2 degrees c, with an optimum at 7 degrees c, cells in steady-state growth at 0 and 13 degrees c appeared to exhibit different patterns in the levels of certain individual proteins. with a shift in temperature, the steady-state level of individual proteins was achieved only after dramatic transient changes in the rates of synthesis of a small number of those proteins whose levels wo ... | 1991 | 1777861 |
| responses to multiple-nutrient starvation in marine vibrio sp. strain ccug 15956. | the response of marine vibrio sp. strain s14 (ccug 15956) to long-term (48-h) multiple-nutrient starvation (i.e., starvation for glucose, amino acids, ammonium, and phosphate simultaneously) can be described as a three-phase process. the first phase, defined as the stringent control phase, encompasses an accumulation of guanosine 5'-diphosphate 3'-diphosphate (ppgpp) and decreases in rna and protein synthesis during the first 40 min. in the second phase, there is a temporary increase in the rate ... | 1990 | 1701428 |
| both the anaerobic pathway and aerobic desaturation are involved in the synthesis of unsaturated fatty acids in vibrio sp. strain abe-1. | vibrio sp. strain abe-1 is a unique marine bacterium in terms of its ability to synthesize delta 9-trans-hexadecenoic acid and delta 7-cis-tetradecenoic acid (14:1(7c); okuyama, h., sasaki, s., higashi, s. and murata, n. (1990) j. bacteriol. 172, 3515-3518). the present study, involving labeling with [1-14c]acetate, demonstrated that 14:1 is synthesized by the anaerobic pathway. when cells of this bacterium were grown in the presence of [1-14c]myristic acid (14:0), this compound was converted to ... | 1992 | 1551444 |
| ribosomes exist in large excess over the apparent demand for protein synthesis during carbon starvation in marine vibrio sp. strain ccug 15956. | carbon starvation induces the development of a starvation- and stress-resistant cell state in marine vibrio sp. strain s14 (ccug 15956). the starved cells remain highly responsive to nutrients during prolonged starvation and exhibit instantaneous severalfold increases in the rates of protein synthesis and rna synthesis when substrate is added. in order to elucidate the physiological basis for the survival of cells that are starved for a long time, as well as the capacity of these cells for rapid ... | 1992 | 1383195 |
| survival, stress resistance, and alterations in protein expression in the marine vibrio sp. strain s14 during starvation for different individual nutrients. | the response of the marine vibrio sp. strain s14 to starvation for carbon, nitrogen, or phosphorus and to simultaneous depletion of all these nutrients (multiple-nutrient starvation) was examined with respect to survival, stress resistance, quantitative and qualitative alterations in protein and rna synthesis, and the induction of the stringent control. of the conditions tested, carbon starvation and multiple-nutrient starvation both promoted long-term starvation resistance and a rapid induction ... | 1992 | 1371661 |
| [non-cholera vibrios in enterobacteriologic routine]. | of 3250 diarrheal stools received for microbiologic diagnosis at a private clinical laboratory in recife, brazil, strains of vibrio were isolated from 55 (1.7%). the study was carried out from may 1989 through may 1991. for recovering vibrio, fecal samples were enriched in alkaline peptone water supplemented with 2% nacl and subcultured on thiosulfate-citrate-bile salts-sucrose agar (tcbs). of the recovered species, v. parahaemolyticus was most commonly found (24 strains), followed by v. furniss ... | 1992 | 1340026 |
| food microbiology update. emerging foodborne pathogens. | a review of three "emerging" foodborne pathogen groups is presented, including campylobacter jejuni/coli, yersinia enterocolitica, and foodborne vibrio sp. | 1983 | 6383214 |
| a role for tumor necrosis factor-alpha in the increased mortality associated with vibrio vulnificus infection in the presence of hepatic dysfunction. | the present study was designed to evaluate whether pre-existing hepatic dysfunction (cirrhosis) leads to increased morbidity and mortality, in part through an inappropriate in vivo tumor necrosis factor-alpha response. | 1996 | 8633922 |
| an analysis of the effect of changes in growth temperature on proteolysis in vivo in the psychrophilic bacterium vibrio sp. strain ant-300. | in the psychrophilic bacterium vibrio sp. strain ant-300, the rate of protein degradation in vivo, measured at fixed temperatures, increased with elevation of the growth temperature. a shift in growth temperature induced a marked increase in this rate. dialysed cell-free extracts hydrolysed exogenous insulin, globin and casein (in decreasing order of activity) but did not hydrolyse exogenous cytochrome c. cells contained at least seven protease separated by deae-sephacel chromatography, one of w ... | 1992 | 1336030 |
| global analysis of the carbon starvation response of a marine vibrio species with disruptions in genes homologous to rela and spot. | the stringent control response, which involves a rapid accumulation of ppgpp, is triggered if the marine vibrio sp. strain s14 is subjected to carbon and energy starvation. by means of high-resolution two-dimensional gel electrophoresis analysis, we addressed the role of the major ppgpp-synthesizing enzyme (rela) in the regulation of the carbon starvation response of vibrio sp. strain s14. the finding that a large number of the carbon starvation-induced proteins were underexpressed in the vibrio ... | 1996 | 8759854 |
| changes in morphology and cell wall structure that occur during growth of vibrio sp. nctc4716 in batch culture. | when grown in batch culture in various media vibrio sp. nctc4716 displayed a distinct sequence of morphological forms. organisms occurred as stout, almost straight rods in exponential phase, curved rods (characteristic of vibrio spp.) in stationary phase, and predominantly as spheres in decline phase. the spheres were formed after growth had ceased due to the depletion of the carbon/energy source. they were not viable, survival of the culture depending on the few rod forms that remained during t ... | 1975 | 803546 |
| extracellular nuclease produced by a marine bacterium. ii. purification and properties of extracellular nuclease from a marine vibrio sp. | extracellular nuclease produced by a marine vibrio sp., strain no. 2, was purified by salting out with ammonium sulfate and by chromatography on a deae-cellulose column and twice on a sephadex g-200 column. the nuclease was eluted as a single peak in which the deoxyribonuclease (dnase) activity and ribonuclease (rnase) activity appeared together. polyacrylamide disc gel electrophoresis showed a single band of stained protein which had both dnase and rnase activity. the molecular weight of the en ... | 1976 | 10061 |
| soft-shell clam, mya arenaria, a convenient laboratory animal for screening pathogens of bivalve mollusks. | attempts to introduce infectious or foreign material into oysters and other bivalve mollusks usually involve force or trauma because of immediate, prolonged adduction of the tightly closing valves. the soft-shell clam, mya arenaria, is unable to seal its valves completely and relaxes readily, exposing soft tissue and a large siphon. this species is free from fouling organisms and is readily available at all seasons in the new england and mid-atlantic areas. suspensions of five strains of vibrio ... | 1971 | 4940871 |
| responses of diverse heterotrophic bacteria to elevated copper concentrations. | the influence of copper on the growth of escherichia coli, pseudomonas aeruginosa, bacillus cereus, bacillus subtilis, vibrio parahaemolyticus, vibrio alginolyticus (three strains), and an unidentified vibrio sp. was examined in batch cultures. the effects of copper at micromolar concentrations varied from undetectable to complete growth inhibition. each strain was able to recover from a growth lag observed after copper addition at a characteristic concentration. copper concentrations that allow ... | 1994 | 8069784 |
| recovery from nutrient starvation by a marine vibrio sp. | a marine psychrophilic vibrio sp., ant-300, recovered from starvation after the addition of 1 volume of complete nutrient medium to 9 volumes of starvation menstruum. turbidity (measured by optical density), viable cell counts, cell size (measured from electron micrographs), and cellular concentrations of protein, dna, and rna were monitored with recovery time. the usual growth curve of bacterial cultures was observed. on a per viable cell basis, protein, dna, and rna increased to maximum values ... | 1983 | 6191662 |
| use of vibrio spp. for expression of escherichia coli enterotoxin b subunit fusion proteins: purification and characterization of a chimera containing a c-terminal fragment of dna polymerase from herpes simplex virus type 1. | the nontoxic b subunit of escherichia coli heat-labile enterotoxin (etxb) is a convenient carrier molecule for the attachment and delivery of heterologous peptides into eukaryotic cells. to evaluate the properties of such etxb-based fusion proteins an efficient method for their production and purification is required. high-level production and purification of native etxb has been achieved using heterologous expression and secretion in a marine vibrio (amin, t., and hirst, t. r., 1994, protein ex ... | 1996 | 8936601 |
| eubacteria-type isocitrate dehydrogenase from an archaeon: cloning, sequencing, and expression of a gene encoding isocitrate dehydrogenase from a hyperthermophilic archaebacterium, caldococcus noboribetus. | a gene coding for isocitrate dehydrogenase (icdh) was cloned from a hyperthermophilic archaebacterium, caldococcus noboribetus, and sequenced. the gene was preceded by a promoter-like sequence and was followed by a terminator-like sequence. the deduced amino acid sequence of c. noboribetus icdh showed high similarities to eubacterial icdh. in particular, extremely high identity scores were found for icdhs from vibrio sp. (48.2%) and escherichia coli (47.9%). the gene was expressed in e. coli by ... | 1996 | 8951037 |
| partial purification of extracellular toxic material of fish vibrio. | hemolytic activity of 3 pathogenic strains, of fish vibrio commonly associated with vibriosis (v anguillarum ncmb6 and ncmb571 strains, and vibrio sp n7802 strain) was examined, using chicken and mammalian erythrocytes. vibrio strains ncmb6 and ncmb571 and their culture filtrates had hemolytic activity against 8 kinds of erythrocytes tested, whereas vibrio strain n7802 produced only a little amount of hemolysin. strain ncmb571 culture filtrate and its material partially purified by column chroma ... | 1984 | 6497124 |
| effects of antimetabolites on the adhesion of an estuarine vibrio sp. to polystyrene. | the effect of various metabolic inhibitors and antibiotics on the adhesion of an estuarine bacterium, vibrio proteolytica, to polystyrene was investigated. cells were either exposed to the substratum and the antimetabolite simultaneously or grown in the presence of a 25% mic and presented the substratum in the absence of the antimetabolite. based on the response elicited, these inhibitors could be divided into three classes: (i) those that had little or no effect on adhesion (fluorodeoxyuridine ... | 1984 | 6508307 |
| substrate specificity of a novel alcohol resistant metalloproteinase, vimelysin, from vibrio sp. t1800. | vimelysin is a novel alcohol resistant metalloproteinase from vibrio sp. t1800. the substrate specificity of vimelysin was studied by using natural and furylacryloyl dipeptide substrates. vimelysin cleaved mainly pro7-phe8 bond and slightly tyr4-ile5 bond in human angiotensin i. vimelysin also cleaved mainly phe24-phe25 and tyr16-leu17 bonds, and slightly his5-leu6, his10-leu11, ala14-leu15, and gly23-phe24 bonds in oxidized insulin b-chain. the substrate specificity of vimelysin, by using furyl ... | 1996 | 8987663 |
| characterization of malate dehydrogenase from deep-sea psychrophilic vibrio sp. strain no. 5710 and cloning of its gene. | a metabolic key enzyme malate dehydrogenase (mdh) was purified from a deep-sea psychrophilic bacterium, vibrio sp. strain no. 5710. the enzyme displayed an optimal activity shifted toward lower temperature and a pronounced heat lability. a gene encoding this enzyme was isolated and cloned. recombinant escherichia coli cells harboring the isolated clone expressed mdh activity with temperature stability identical to that of the parental psychrophile. nucleotide sequencing of the gene revealed that ... | 1996 | 8998993 |
| novel extracellular alkaline metalloendopeptidases from vibrio sp. nuf-bpp1: purification and characterization. | we found two types of novel alkaline metalloendopeptidases (ap1 and ap2) from a marine bacterium, isolated from the intestine of a five-barred goatfish (parupeneus trifasciatus) and identified as vibrio sp. (nuf-bpp1). we studied the structure-function relationship of these marine bacterial proteases. the electrophoretically homogeneous proteases had a molecular mass of 48 kda for ap1 and 36 kda for ap2 on sds-page, and showed optimum activity at around ph 9.5-10.0 (casein as substrate). calcium ... | 1997 | 9028037 |
| comparative studies and laboratory diagnosis of vibrio vulnificus, an invasive vibrio sp. | vibrio vulnificus was isolated from a bacteremic patient. this strain, together with other isolates of v. vulnificus, was compared with v. alginolyticus, v. fluvialis, and v. parahaemolyticus with regard to growth characteristics on enteric agar media (enabling isolation and identification) and production of exoenzymes which could correlate with invasive potential. v. vulnificus grew well on macconkey. endo, xylose-lysine deoxycholate, and hektoen enteric agar plates. because v. vulnificus colon ... | 1984 | 6699140 |
| isolation of a carbon starvation regulatory mutant in a marine vibrio strain. | a carbon starvation-responding lac fusion of the marine vibrio sp. strain s14 was used as a reporter strain in order to identify genes critical in the regulation of the carbon starvation response. interestingly, sequence data together with an altered phenotype with respect to the accumulation of guanosine 3',5'-bispyrophosphate (ppgpp) imply that one of the genes (csrs) identified by this approach is an escherichia coli spot equivalent. complementary data suggest that the function encoded by the ... | 1995 | 7592494 |
| a thermolabile triosephosphate isomerase from the psychrophile vibrio sp. strain ant-300. | we report the isolation of a gene encoding triosephosphate isomerase (tim; ec 5.3.1.1) from vibrio sp. strain ant-300, a psychrophilic marine eubacterium that grows optimally at 7 degrees c. the deduced primary sequence of this isomerase is 50% identical to escherichia coli tim and 37% identical to the isomerase of the psychrotroph moraxella sp. ta137. transformation with this gene allowed growth of a tim-deficient e. coli strain on selective media, but only at temperatures below 30 degrees c. t ... | 1995 | 7639513 |
| cloning, sequence analysis, expression, and inactivation of the corynebacterium glutamicum icd gene encoding isocitrate dehydrogenase and biochemical characterization of the enzyme. | nadp(+)-dependent isocitrate dehydrogenase (icd) is an important enzyme of the intermediary metabolism, as it controls the carbon flux within the citric acid cycle and supplies the cell with 2-oxoglutarate and nadph for biosynthetic purposes. in the amino acid-producing organism corynebacterium glutamicum, the specific activity of icd was independent of the growth substrate and of the growth phase at approximately 1 u/mg, indicating that this enzyme is constitutively formed. the icd gene, icd, w ... | 1995 | 7836312 |
| glucose upshift of carbon-starved marine vibrio sp. strain s14 causes amino acid starvation and induction of the stringent response. | the physiological status of carbon-starved cells of the marine vibrio sp. strain s14 has been investigated by the analysis of their immediate response to carbon and energy sources. during the first minute after glucose addition to 48-h-starved cells, the pools of atp and gtp increased rapidly, and the [atp]/[adp] ratio reached the level typical for growing cells within 4 min. the total rates of rna and protein synthesis increased initially but were inhibited 4 to 5 min after glucose addition by ... | 1994 | 7928949 |
| stringent control during carbon starvation of marine vibrio sp. strain s14: molecular cloning, nucleotide sequence, and deletion of the rela gene. | in order to evaluate the role of the stringent response in starvation adaptations of the marine vibrio sp. strain s14, we have cloned the rela gene and generated relaxed mutants of this organism. the vibrio rela gene was selected from a chromosomal dna library by complementation of an escherichia coli delta rela strain. the nucleotide sequence contains a 743-codon open reading frame that encodes a polypeptide that is identical in length and highly homologous to the e. coli rela protein. the amin ... | 1994 | 7928955 |
| reductive and hydrolytic activities of enteric bacteria on food colours. | reductive and hydrolytic activities on two food colours namely metanil yellow and indigo carmine by different strains of enteric bacteria were evaluated. enteric bacteria possessed both reductive and hydrolytic capabilities which depended upon the incubation period and the type of strain. in certain bacterial strains, (escherichia coli and vibrio sp.), reductive activity was visible after 3 hr. other enteric strains showed an increasing activity right from the beginning of incubation. enterobact ... | 1997 | 9475071 |
| purification and characterization of a novel enzyme, alpha-neoagarooligosaccharide hydrolase (alpha-naos hydrolase), from a marine bacterium, vibrio sp. strain jt0107. | a novel enzyme, alpha-neoagarooligosaccharide hydrolase (ec 3.2.1.-), which hydrolyzes the alpha-1,3 linkage of neoagarooligosaccharides to yield agaropentaose (o-beta-d-galactopyranosyl(1-->4)-o-3,6-anhydro-alpha-l-galactopyranosyl (1-->3)-d-galactose], agarotriose [o-beta-d-galactopyranosyl(1-->4)-o-3,6-anhydro- alpha-l-galactopyranosyl (1-->3)-d-galactose], agarobiose [o-beta-d-galactopyranosyl(1-->4)-3,6-anhydro-l-galactose], 3,6-anhydro-l-galactose, and d-galactose was isolated from the mar ... | 1994 | 7961439 |
| restriction endonucleases from selenomonas ruminantium which recognize and cleave 5'-at/taat-3'. | two natural isolates from fallow-deer rumen identified as selenomonas ruminantium were found to produce a restriction endonuclease which we called sru4di. this enzyme was isolated from cell extracts by phosphocellulose chromatography. analysis of the sru4di recognition site showed that sru4di recognizes the hexanucleotide sequence 5'-at/taat-3' generating 5'dinucleotide protruding ends upon cleavage and thus is a true isoschizomer of vspi, a restriction enzyme isolated from vibrio sp. | 1994 | 8042908 |
| use of green fluorescent protein to tag and investigate gene expression in marine bacteria. | two broad-host-range vectors previously constructed for use in soil bacteria (a. g. matthysse, s. stretton, c. dandie, n. c. mcclure, and a. e. goodman, fems microbiol. lett. 145:87-94, 1996) were assessed by epifluorescence microscopy for use in tagging three marine bacterial species. expression of gfp could be visualized in vibrio sp. strain s141 cells at uniform levels of intensity from either the lac or the npt-2 promoter, whereas expression of gfp could be visualized in psychrobacter sp. st ... | 1998 | 9647829 |
| sequence analysis of the agab gene encoding a new beta-agarase from vibrio sp. strain jt0107. | an agarase gene (agab) was cloned from genomic dna of vibrio sp. strain jt0107. analysis of the 3200 nucleotide sequence just before the agarase 0107 gene (agaa) which existed in genomic dna of vibrio sp. strain jt0107 revealed a putative single open reading frame coding for 955 amino acids. comparison of the deduced amino acid sequence of agab to that of agarase 0107 revealed the existence of partially highly homologous regions. a part of this gene was expressed in escherichia coli to yield a p ... | 1994 | 8193156 |
| immunofluorescent detection of ice-ice disease-promoting bacterial strain vibrio sp. p11 of the farmed macroalga, kappaphycus alvarezii (gigartinales, rhodophyta). | a specific immunofluorescent probe consisting of polyclonal antibodies was developed to detect a marine bacterium, vibrio sp. strain p11, which was found in a previous study to promote the ice-ice disease in the cultivated red macroalga, kappaphycus alvarezii. the method involves a combined application of the fluorescent stains, 4',6-diamidino-2-phenylindole (dapi) and the p11 pabs, into a homogenized seaweed sample (<1 g wet wt), which is prediluted to make bacteria countable under an epifluore ... | 1998 | 9701641 |
| genes encoding two isocitrate dehydrogenase isozymes of a psychrophilic bacterium, vibrio sp. strain abe-1. | the genes coding for two structurally different isocitrate dehydrogenase isozymes (idh-i and idh-ii) of a psychrophilic bacterium, vibrio sp. strain abe-1, were cloned and sequenced. open reading frames of the genes (icdi and icdii) are 1,248 and 2,229 bp in length, respectively. the amino acid sequences predicted from the open reading frames of icdi and icdii corresponded to the n-terminal amino acid sequences of the purified idh-i and idh-ii, respectively. no homology was found between the ded ... | 1993 | 8226630 |
| diarrhea among expatriate residents in thailand: correlation between reduced campylobacter prevalence and longer duration of stay. | background: the etiology of diarrhea among expatriates in thailand was investigated. methods: one hundred and five stool specimens were collected from americans and europeans who had developed diarrhea while residing in thailand. these stools were analyzed for bacterial enteric pathogens. results: enterotoxigenic escherichia coli was isolated from 18 (17%) persons and campylobacter from 10 (10%). shigella and salmonella were each isolated from 8 (8%) persons. vibrio sp., enteroinvasive escherich ... | 1996 | 9815428 |
| cloning and sequencing of agaa, a unique agarase 0107 gene from a marine bacterium, vibrio sp. strain jt0107. | an agarase gene (agaa) was cloned from genomic dna of vibrio sp. strain jt0107. an open reading frame of 2,985 nucleotides gave a primary translation product composed of the mature protein, agarase 0107 (975 amino acid residues, with a molecular weight of 105,271) and a signal peptide of 20 amino acid residues at the n terminus. comparison of the deduced amino acid sequence of agarase 0107 with those of streptomyces coelicolor and pseudomonas atlantica suggests that these enzymes share two regio ... | 1993 | 8285681 |
| evidence for cis-trans isomerization of a double bond in the fatty acids of the psychrophilic bacterium vibrio sp. strain abe-1. | vibrio sp. strain abe-1 was grown in a medium that contained as its stable isotope tracer either [2,2-2h2]cis-9-hexadecenoic or [2,2-2h2]trans-9-hexadecenoic acid. gas chromatographic-mass spectrometric analysis of the cis-9-hexadecenoic and trans-9-hexadecenoic acid fractions from the cells revealed the formation of an intracellularly isomerized 2,2-2h2-fatty acid which differed from the tracer only in the geometrical configuration of the double bond. this observation shows that cis-trans isome ... | 1993 | 8423164 |
| vibrio viscosus in farmed atlantic salmon salmo salar in scotland: field and experimental observations. | winter mortality occurred in market-sized (2 to 3 kg) atlantic salmon salmo salar reared in sea cages in scottish waters. many of the fish had skin ulcers. internally prominent dark-brown petechiae or ecchymotic haemorrhage was observed. splenomegaly was associated with congestion and widespread necrosis. a vibrio sp. was isolated from internal organs. biochemically isolates of the bacterium were similar to a previously described bacterium, vibrio viscosus, recorded in a phenotypic study from fa ... | 1998 | 9891731 |
| jamu gendong, a kind of traditional medicine in indonesia: the microbial contamination of its raw materials and endproduct. | an examination on the microbiological quality of seven kinds of jamu gendong (jg) and their raw materials has been conducted according to the requirements of microbial contamination in traditional medicine, issued by the department of health of indonesia in 1986. samples of jg and their raw materials were taken from producers in three districts of surabaya. the samples were subject to the following examinations: total plate count (tpc), mpn coliform, the enumeration of molds and yeasts, the pres ... | 1998 | 10030724 |
| purification and characterization of a new agarase from a marine bacterium, vibrio sp. strain jt0107. | a marine bacterial strain that decomposes the cell walls of some seaweeds, including a laminaria sp. and undaria pinnatifida, has been isolated from seawater. this strain has been classified to the genus vibrio. one of the enzymes which the bacteria secreted into the culture medium was isolated and purified 45-fold from the culture fluid by a combination of ammonium sulfate precipitation and successive rounds of anion-exchange column chromatography. purified protein migrated as a single band (m( ... | 1993 | 8517750 |
| nh4+ transport system of a psychrophilic marine bacterium, vibrio sp. strain abe-1. | nh4(+) transport system of a psychrophilic marine bacterium vibrio sp. strain abe-1 (vibrio abe-1) was examined by measuring the uptake of [14c]methylammonium ion (14ch3nh3+) into the intact cells. 14ch3nh3+ uptake was detected in cells grown in medium containing glutamate as the sole nitrogen source, but not in those grown in medium containing nh4cl instead of glutamate. vibrio abe-1 did not utilize ch3nh3+ as a carbon or nitrogen source. nh4cl and nonradiolabeled ch3nh3+ completely inhibited 1 ... | 1999 | 10356994 |
| purification and characterization of an extracellular beta-1,4-mannanase from a marine bacterium, vibrio sp. strain ma-138. | a beta-mannanase (ec 3.2.1.78) from vibrio sp. strain ma-138 was purified by ammonium sulfate precipitation and several chromatographic procedures including gel filtration, adsorption, and ion-exchange chromatographies. the final ion-exchange chromatography mono q yielded one major active fraction and three minor active fractions. the major active fraction was purified to homogeneity on the basis of native polyacrylamide gel electrophoresis (page). this purified enzyme was identified as a glycop ... | 1995 | 8534110 |
| identification of inadequately cleaned equipment used in a sheep carcass-breaking process. | aeromonads deposited on pork during a carcass-breaking process were recovered on hydrophobic grid membrane filters placed on ampicillin-dextrin-ethanol agar. isolates from 85 honey-yellow colonies from filters on that medium were aeromonas hydrophila (95%) or vibrio sp. (5%). presumptive aeromonads, coliforms, and escherichia coli in swab samples from product passing through a sheep carcass-breaking process were enumerated by hydrophobic grid membrane filtration techniques with a detection level ... | 1999 | 10382653 |
| determination of total protein content of bacterial cells by sypro staining and flow cytometry. | an assay has been developed for measuring protein biomass of marine planktonic bacteria by flow cytometry. the method was calibrated by using five species of bacteria (an arcobacter sp., a cytophaga sp., an oceanospirillum sp., a pseudoalteromonas sp., and a vibrio sp.) recently isolated from seawater samples and grown in culture at different temperatures. the intensity of sypro-protein fluorescence of these bacteria strongly correlated with their total protein content, measured by the bicinchon ... | 1999 | 10388732 |
| synthesis of immediate upshift (iup) proteins during recovery of marine vibrio sp. strain s14 subjected to long-term carbon starvation. | proteins induced during the initial phase of recovery after long-term carbon starvation in the marine vibrio sp. strain s14 were identified by two-dimensional gel electrophoresis analysis. nutritional upshift experiments with pulse-labeled cells were performed after addition of glucose to cells starved for 48 h. eighteen proteins synthesized during the first 3 min after substrate addition were identified and designated immediate upshift proteins (iup proteins). they were induced at least 10-fold ... | 1996 | 8550518 |
| a rela/spot homologous gene from streptomyces coelicolor a3(2) controls antibiotic biosynthetic genes. | a 0.972-kilobase pair dna fragment from streptomyces lividans that induces the production of the blue-pigmented antibiotic actinorhodine in s. lividans when cloned on a multicopy plasmid has led to the isolation of a 4-kilobase pair dna fragment from streptomyces coelicolor containing homologous sequence. computer-assisted analysis of the dna sequence revealed three putative open reading frames (orfs), orf1, orf2, and orf3. orf2 extends beyond the sequenced dna fragment, and its deduced product ... | 1996 | 8631867 |
| the dnak homologue of the marine vibrio sp. strain s14 binds to the unprocessed form of a carbon starvation-specific periplasmic protein. | the escherichia coli dnak homologue in vibrio sp. strain s14 was shown to possess chaperone function for translocation during carbon starvation. this was demonstrated by using the method of co-immunoprecipitation. dnak co-precipitated with the carbon starvation-specific periplasmic space protein csp5 three hours after the onset of carbon starvation. pulse-chasing of the protein with radiolabelled methionine followed by the addition of an excess of unlabelled methionine demonstrated that the csp5 ... | 1994 | 7912811 |
| polynucleotide sequence relationships among japanese and american strains of vibrio parahaemolyticus. | polynucleotide sequence relationships between two reference vibrio parahaemolyticus strains isolated from japanese and american gastroenteritis patients were investigated by use of (32)p-dna/dna reassociation in free solution. in addition, these strains were similarly compared with 22 other strains of estuarine and marine vibrios, including 11 strains previously identified as v. parahaemolyticus (2 japanese, 1 of unknown location, and 8 american strains obtained from diverse geographical locatio ... | 1973 | 4712574 |
| recent developments in fish vaccinology. | during the last 10 to 20 years vaccination has become established as an important method for prevention of infectious diseases in farmed fish, mainly salmonid species. so far, most commercial vaccines have been inactivated vaccines administered by injection or immersion. bacterial infections caused by gram-negative bacteria such as vibrio sp., aeromonas sp., and yersinia sp. have been effectively controlled by vaccination. with furunculosis, the success is attributed to the use of injectable vac ... | 1999 | 10614510 |
| purification and characterization of beta-1,3-xylanase from a marine bacterium, vibrio sp. xy-214. | beta-1,3-xylanase was purified to gel electrophoretic homogeneity and 83-fold from a cell-free culture fluid of vibrio sp. xy-214 by ammonium sulfate precipitation and successive chromatographies. the enzyme had a pl of 3.6 and a molecular mass of 52 kda. the enzyme had the highest level of activity at ph 7.0 and 37 degrees c. the enzyme activity was completely inhibited by cu2+, hg2+, and n-bromosuccinimide. the enzyme hydrolyzed beta-1,3-xylan to produce mainly xylotriose and xylobiose but did ... | 1999 | 10635569 |
| complicated suppurative otitis media in a greek diver due to a marine halophilic vibrio sp. | halophilic vibrios are distinct from non-cholera vibrios and have been recognized increasingly as potentially pathogenic bacteria in extraintestinal infections. a case of suppurative chronic otitis media in a greek diver with vibrio alginolyticus recovered from an ear drainage culture, is reported. the patient received appropriate antimicrobial therapy and her hearing improved significantly after a tympanoplasty type i was performed. the association of halophilic vibrio spp. infections with prol ... | 1995 | 8551126 |
| quality of water--quality of life. | especially in developing countries, the problem of adequate drinking water supply is an ever growing one. public health programmes have been established to improve the population's health conditions, but these programmes require big financial means for guaranteeing adequate supply of potable water and medical therapy for sick people. too little emphasis is still put on regular testing of drinking water for microorganisms such as aeromonas sp. and vibrio sp. in a spot check analysis in various co ... | 1999 | 10659386 |
| [clinical study on bacteremia in patients with liver cirrhosis]. | infections and fever are frequent in patients with liver cirrhosis. study on bacteremia in cirrhotic patients has not been reported in japan. in a 16 year period from 1979 to 1994, we collected 39 cases with 40 episodes and 44 microorganisms of bacteremia for this study. the incidence of bacteremia in cirrhotic hospital admissions was 4.8% (39/808). gram negative bacteria were the predominant microorganisms of bacteremia (66%, 29/44). among them, escherichia coli, klebsiella pneumoniae, vibrio s ... | 1996 | 8699093 |
| kinetic characterization of the neutral protease vimelysin from vibrio sp. t1800. | the kinetics of the hydrolysis of dipeptide and tripeptide substrates by the recently discovered neutral protease from vibrio species t1800 (vimelysin) were studied. in the ph dependence of the apparent second-order rate constant, the pka2 value of vimelysin (approximately 6.5) was significantly lower than thermolysin (8.3), although the pka1 (approximately 5.1) values were comparable (5.0). the kcat/km(lim) parameter for hydrolysis of fua-gly-phenh2 (fua = furylacryloyl) was more than sevenfold ... | 1996 | 8917432 |
| identification and characterization of an extracellular protease activity produced by the marine vibrio sp. 60. | the marine fish pathogen vibrio sp. 60 has been used as a host for heterologous expression of the escherichia coli heat-labile enterotoxin b-subunit and derivatives carrying a c-terminal extension. in this study, a chimeric enterotoxin b-subunit with an extension corresponding to the carboxy-terminal nine amino acids -tyr-ala-gly-ala-val-val-asn-asp-leu-cooh from the small subunit of herpes simplex virus type 1-encoded ribonucleotide reductase, is shown to be proteolytically cleaved in the extra ... | 1996 | 8919453 |
| heat-labile bacterial alkaline phosphatase from a marine vibrio sp. | psychrophilic organisms have successfully adapted to various low-temperature environments such as cold ocean waters. catalysts with increased catalytic efficiencies are produced, generally at the expense of thermal stability due to fewer non-covalent stabilizing interactions. a marine bacterial strain producing a particularly heat-labile alkaline phosphatase was selected from a total of 232 strains isolated from north-atlantic coastal waters. from partial 16s rrna sequences the strain was charac ... | 2000 | 10862903 |
| isocitrate dehydrogenase from thermus aquaticus yt1: purification of the enzyme and cloning, sequencing, and expression of the gene. | isocitrate dehydrogenase from an extremely thermophilic bacterium, thermus aquaticus yt1, was purified to homogeneity, and the gene was cloned by using a degenerate oligonucleotide probe based on the n-terminal sequence. the gene consisted of a single open reading frame of 1,278 bp preceded by a shine-dalgarno ribosome binding site, and a terminator-like sequence was detected downstream of the open reading frame. the g+c content of the coding region was 65%, and that of the third nucleotide of t ... | 1996 | 8953733 |
| [recent advances in tetrodotoxin research]. | one century has passed since fugu toxin was named tetrodotoxin (ttx) by tahara. chemical problems such as crystallization of tetrodotoxin and subsequent structure determination were solved by research groups headed by tsuda, hirata, woodward, and mosher. the international symposium on the chemistry of natural products in kyoto (1964) was well known as symposium which the structure of ttx was internationally clarified. since the first isolation of toxin from taricha torosa (imori) as natural sour ... | 2000 | 11082696 |