Publications

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vectors for expression and amplification of cdna in mammalian cells: expression of rat phenylalanine hydroxylase.we have constructed two recombinant plasmid vectors for direct expression and amplification of cdna in mammalian cells. each vector carries two dominant selectable markers (the bacterial neo gene and the mouse dhfr gene), a promoter sequence (viral ltr in pav009/a+, and sheep metallothionein promoter in pmt010/a+), a polyadenylation signal sequence, and a bam hi site to allow insertion of cdna. we have used these vectors to prepare recombinant clones for the expression of rat phenylalanine hydro ...19863028740
scalp cooling by cold air for the prevention of chemotherapy-induced alopecia.a new system is described for cooling the scalp with cold air in order to prevent chemotherapy-induced alopecia. compressed air was cooled by means of a vortex tube built into a hair-drier cap. this system reduced the blood flow in the scalp to 35%, the surface temperature to 14.2 degrees c and the intradermal temperature at hair follicle level to 29.2 degrees c. the low temperature could be kept constant for at least one hour of cooling. by means of comparison, with cryogel packs the lowest epi ...20072074916
a new vibrio species, vibrio cincinnatiensis, causing meningitis: successful treatment in an adult. 19863510054
entry of vibrio cincinnatiensis into viable but nonculturable state and its resuscitation.the aim was to characterize the viable but nonculturable (vbnc) state of vibrio cincinnatiensis and its resuscitation.200919196443
[2 cases of sudden death of twins with idiopathic cardiomegaly]. 2002129892
bacteria of the gamma-subclass proteobacteria associated with zooplankton in chesapeake bay.the seasonal abundance of gamma-subclass proteobacteria, vibrio-photobacterium, vibrio cholerae-vibrio mimicus, vibrio cincinnatiensis, and vibrio vulnificus in the choptank river of chesapeake bay associated with zooplankton was monitored from april to december 1996. large (>202- microm) and small (64- to 202- microm) size classes of zooplankton were collected, and the bacteria associated with each of the zooplankton size classes were enumerated by fluorescent oligonucleotide direct count. larg ...200212406743
seasonality of chesapeake bay bacterioplankton species.bacteria, gamma-subclass of proteobacteria, vibrio-photobacterium, vibrio vulnificus, vibrio cholerae-vibrio mimicus, and vibrio cincinnatiensis in water samples collected from the choptank river in chesapeake bay from 15 april to 16 december 1996 were enumerated using a fluorescent oligonucleotide direct-counting (fodc) procedure. fodc results obtained using a bacteria taxon-specific probe ranged from one-third the number of to the same number as that obtained by the acridine orange direct coun ...200212406742
structure of the rabbit phospholamban gene, cloning of the human cdna, and assignment of the gene to human chromosome 6.we have isolated and characterized genomic dna clones encoding rabbit phospholamban. only a single gene for phospholamban was detected in the rabbit genome. the phospholamban gene of 13.2 kilobases contains only one 10.5-kilobase intron, which separates exonic sequences located in the 5'-untranslated region. two potential transcription initiation sites were mapped to 335 and 185 nucleotides upstream from the translation initiation site in the mrna or 239 and 89 nucleotides upstream from the exon ...19911828805
steroids in asthma. 200091910
[biochemical aspect in cardiac ischemia--ischemic changes and reperfusion injury]. 20102838873
contribution to some phenotypical characteristics of vibrio cincinnatiensis. studies in one strain of a diarrhoeic human patient and in two isolates from aborted bovine fetuses.until now, only a few strains of v. cincinnatiensis have been isolated. this study describes a further three isolates which originated in one case from a stool specimen of an immunocompromised elder patient suffering from enteritis and in two cases from the rennin stomachs of aborted bovine fetuses. these strains grew on tcbs, cin, macconkey and xld plates. their biochemical activities were dependent on nacl concentration, in particular the formation of indole, lysine and ornithine decarboxylase ...19938305803
antimicrobial resistance and resistance genes in aerobic bacteria isolated from pork at slaughter.the aim of this study was to investigate the phenotypic and genotypic antimicrobial resistance, integrons, and transferability of resistance markers in 243 aerobic bacteria recovered from pork at slaughter in the people's republic of china. the organisms belonged to 22 genera of gram-negative bacteria (92.2%) and gram-positive bacteria (7.8%). high levels of resistance were detected to tetracycline, trimethoprim-sulfamethoxazole, and ampicillin (36.2 to 54.3%), and lower levels were detected to ...201627052863
cervical lymph node metastases with occult primary.patients with cervical metastases from an unknown primary tumour present both diagnostic and therapeutic problems. twenty patients with a metastatic lesion in the neck, in whom the primary tumour was not found despite extensive, diagnostic procedures, were treated in the department of otolaryngology, linköping university hospital, during a 14-year period (1971-1984). since 1975 tonsillectomy has been performed regularly on the affected side as a part of the diagnostic procedure. eight patients w ...20123815873
vibrio cincinnatiensis sp. nov., a new human pathogen.a halophilic gram-negative rod was isolated from blood and cerebrospinal fluid collected from a 70-year-old male having no known contact with seafood or salt water. positive biochemical tests included oxidase, sensitivity to 0/129, o-nitrophenyl-beta-d-galactopyranoside, lysine decarboxylase and fermentation of glucose, salicin, n-inositol, sucrose, l-mannose, l-arabinose, and arbutin. negative tests included indole, ornithine decarboxylase, arginine dihydrolase fermentation of lactose, and prod ...19862422196
complement-mediated killing of vibrio species by the humoral fluids of amphioxus branchiostoma belcheri: implications for a dual role of o-antigens in the resistance to bactericidal activity.the functional properties of complement in invertebrate deuterostomes are rather ill-defined. here we showed that the humoral fluids from amphioxus branchiostoma belcheri were capable of causing lysis of some vibrio species including vibrio alginolyticus hw284, vibrio parahaemolyticus hw458 and vibrio harvey sf-1, the first such data in the invertebrate deuterostomes. the fluid bacteriolytic activity was abolished by pre-incubation with heat-inactivated rabbit anti-human c3 serum, heating at 45 ...200818178103
vibrio injenensis sp. nov., isolated from human clinical specimens.vibrio species are well known as motile, mostly oxidase-positive, facultative anaerobic gram-negative bacteria. they are abundant in aquatic environments and are a common cause of human infections including diarrhea, soft tissue diseases, and bacteremia. here, two gram-negative bacteria, designated m12-1144(t) and m12-1181, were isolated from human clinical specimens and identified using a polyphasic taxonomic approach. phylogenetic study based on 16s rrna gene sequence analysis revealed that th ...201728012139
[new studies on a relationship between hemoglobins and rhesus factors in africans]. 20144308643
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