| immunochemical properties of oligosaccharide-protein conjugates with klebsiella-k2 specificity. i. specificity and crossreactivity of anti-conjugate versus anti-bacterial antibodies. | | 1979 | 86152 |
| [cutaneous leishmaniasis. alcoy: an endemic zone]. | | 1979 | 92178 |
| viability and endogenous substrates used during starvation survival of rhodospirillum rubrum. | cells of rhodospirillum rubrum were grown photoorganotrophically and chemoorganotrophically and then starved for organic carbon and combined nitrogen under four conditions: anaerobically in the light and dark and aerobically in the light and dark. illumination prolonged viability and suppressed the net degradation of cell material of phototrophically grown cells, but had no effect on chemotrophically grown cells that did not contain bacteriochlorophyll. the half-life survival times of carbohydra ... | 1978 | 96087 |
| changes in composition of tears of malnourished children. | | 1978 | 98738 |
| cooperative binding of mn2+ and non-cooperative binding of mg2+ to beta-galactosidase (e. coli). | | 1978 | 100107 |
| implications for adult roles from differential styles of mother-infant bonding: an ethological study. | ethological observations of maternal and infant behaviors of nine vervet monkey pairs (cercopithecus aethiops sabaeus) showed the effects of differential styles of early maternal responsiveness on later infant competence. those infants receiving the least amount of maternal responsiveness and the most time-off the mother in the first 3 months of development were more socially competent at 6 months of age. the results are discussed within current ethological "attachment" theories. the detachment ... | 1979 | 105083 |
| demonstration and characterization of specific binding sites for factor viii/von willebrand factor on human platelets. | the presence of specific factor viii/von willebrand factor (fviii/vwf) binding sites on human platelets has been demonstrated by using 125i-fviii/vwf and washed human platelets. binding is ristocetin-dependent and increases in proportion to the concentration of ristocetin from 0.2 to 1 mg/ml. binding of 125i-fviii/vwf to platelets can be competitively inhibited by unlabeled human or bovine fviii/vwf, but not by human thrombin, fibrinogen, alpha 2-macroglobulin, equine collagen, or a lectin of ri ... | 1979 | 108291 |
| nuclear thyroid hormone receptors: evidence for association with nucleolar chromatin. | | 1975 | 173323 |
| towards a phylogeny of the genus vibrio based on 16s rrna sequences. | the inter- and intrageneric relationships of the genus vibrio were investigated by performing a comparative analysis of the 16s rrnas of 10 species, including four pathogenic representatives. the results of immunological and 5s rrna studies were confirmed in that the genus is a neighboring taxon of the family enterobacteriaceae. with regard to the intrageneric structure, vibrio alginolyticus, vibrio campbellii, vibrio natriegens, vibrio harveyi, vibrio proteolyticus, vibrio parahaemolyticus, and ... | 1992 | 1371064 |
| ultrastructural alterations of brain cortex in rat following intraperitoneal administration of mercuric chloride. | intraperitoneal administration of a single dose (6 mg/kg body weight) of mercuric chloride (hgcl2) results in ultrastructural changes in brain cortex of the rats. 18 hours after administration of hgcl2 the accumulation of dense deposits of mercury in nerve and glial cell cytoplasm was observed. the quantity of microglia in neuropil was also increased in this experimental group. we postulate that these cerebral macrophages can play an important role in the process of intoxication, too. 5 days aft ... | 1992 | 1479187 |
| identification of vibrio vulnificus o serovars with antilipopolysaccharide monoclonal antibody. | a serotyping scheme for vibrio vulnificus predicated on the detection of lipopolysaccharide (lps) antigens is proposed. the serovar o typing scheme used to type v. vulnificus employs polyclonal antisera raised in rabbits immunized with heat-killed whole-cell vaccines. polyclonal typing sera produced in this manner cross-react with heterologous strains. affinity purification of polyclonal antisera with lps affinity columns resolved some of these cross-reactions; however, affinity-purified polyclo ... | 1991 | 1761690 |
| analysis of the gene of vibrio hollisae encoding the hemolysin similar to the thermostable direct hemolysin of vibrio parahaemolyticus. | the gene (designated as vh-tdh) of vibrio hollisae 9041 encoding a hemolysin similar to the thermostable direct hemolysin (tdh) of v. parahaemolyticus contained a 567-base-pair open reading frame (orf), which was 93.3-93.5% homologous to those of the tdh genes of v. parahaemolyticus, v. cholerae non-01, and v. mimicus encoding tdh or similar hemolysins. comparative analysis of the nucleotide sequence containing the vh-tdh orf with published nucleotide and amino acid sequences suggested that the ... | 1991 | 1884983 |
| properties of a hemolysin related to the thermostable direct hemolysin produced by a kanagawa phenomenon negative, clinical isolate of vibrio parahaemolyticus. | a hemolytic toxin (vp-trh) produced by a kanagawa phenomenon negative, clinical isolate of vibrio parahaemolyticus was further characterized. the purified vp-trh showed various biological activities, such as fluid accumulation in rabbit ileal loops, increase of rabbit skin vascular permeability, and cardiotoxicity on cultured myocardial cells, all of which are essentially similar to the activities found with thermostable direct hemolysin (vp-tdh), a pathogenic toxin produced by kanagawa phenomen ... | 1990 | 2118824 |
| comparison of hemolysins of vibrio cholerae non-o1 and vibrio hollisae with thermostable direct hemolysin of vibrio parahaemolyticus. | hemolysin (vh-rtdh) produced by vibrio hollisae and hemolysin (nag-rtdh) produced by vibrio cholerae non-o1 were characterized and compared with hemolysin (vp-tdh) produced by vibrio parahaemolyticus. these three hemolysins are each composed of two subunits and have similar, but not identical, molecular weights. the amino acid compositions of vp-tdh and nag-rtdh are similar, but are different from that of vh-rtdh. the three hemolysins showed similar lethal toxicities to mice. the effects of temp ... | 1988 | 2466545 |
| homogeneity of a hemolysin(vh-rtdh) produced by clinical and environmental isolates of vibrio hollisae. | the characteristics of vh-rtdh, a hemolysin similar to vp-tdh of vibrio parahaemolyticus produced by clinical and environmental isolates of vibrio hollisae, were comparatively studied. all 7 strains of v. hollisae tested were found to produce indistinguishable vh-rtdh when they were examined by heat-stability test, western blotting analysis and conventional polyacrylamide slab gel electrophoresis. | 1989 | 2513246 |
| hydrogen peroxide mutagenicity towards salmonella typhimurium. | in bioassays conducted under controlled, comparable conditions, weak direct mutagenicity responses were observed for hydrogen peroxide in the standard (ames test) agar plate incorporation bioassay with salmonella typhimurium strains ta97, ta98, ta102, and ta1537, in a 20 min preincubation test with strains ta97, ta98, ta100, ta102, ta1537, and ta1538, and in a liquid incubation modification using strain ta1537. these results conclusively demonstrate that hydrogen peroxide is a weak mutagen, espe ... | 1989 | 2572065 |
| evidence of immunologic cross-reactivity between hemolysins of vibrio hollisae and vibrio parahaemolyticus demonstrated by monoclonal antibodies. | | 1989 | 2584761 |
| comparative amino acid sequence analysis of hemolysins produced by vibrio hollisae and vibrio parahaemolyticus. | vibrio hollisae produces a hemolysin (vh-rtdh) that is related to the thermostable direct hemolysin of vibrio parahaemolyticus (vp-tdh). although both hemolysins are essentially similar biologically and immunologically, they differ markedly in heat stability; vp-tdh is heat stable, whereas vh-rtdh is heat labile. to elucidate the relationships between their characteristics and molecular structures, we analyzed the amino acid sequence of vh-rtdh and compared it with that of vp-tdh. vh-rtdh consis ... | 1989 | 2592352 |
| dopamine receptors in rabbit and rat eye: characterization and localization of da1 and da2 binding sites. | in vitro autoradiography is used to characterize and localize da1 and da2 receptors in rabbit and rat eyes. the bindings of 125i-sch 23982 (da1 antagonist) and of 125i-iodosulpride (da2 antagonist) to slide mounted tissue sections take place with characteristics expected of substances that recognize da1 and da2 receptors respectively. they are saturable, have high affinity and exhibit an appropriate pharmacology. the regional distribution of these receptors indicates that they are mainly present ... | 1989 | 2707038 |
| heterogeneity of fast-oxidative muscle fibers of chicken demonstrated by anti-myosin monoclonal antibodies. | the fiber type composition of two fast muscles of the chicken, namely, adductor superficialis (as) and pectoralis major (pm) was examined by the histochemical myosin atpase staining and immunochemical techniques using monoclonal antibodies (mcabs). two new mcabs produced against the myosin of the anterior latissimus dorsi (ald) muscle of the chicken and named ald-122 and ald-83 were characterized to be specific for myosin heavy chain (mhc) and for myosin light chain-1 respectively. they were use ... | 1989 | 2737924 |
| purification and partial characterization of a vibrio hollisae hemolysin that relates to the thermostable direct hemolysin of vibrio parahaemolyticus. | hemolysin produced by vibrio hollisae (vh-rtdh), which is related to the thermostable direct hemolysin (vp-tdh) of vibrio parahaemolyticus, was studied. vh-rtdh was purified by successive column chromatographies on diethylaminoethyl-cellulose and an immunoaffinity column coupled with anti vp-tdh immunoglobulin. the purified toxin was homogeneous, as demonstrated by conventional and sodium dodecyl sulfate--polyacrylamide gel electrophoresis (page). the molecular weight of vh-rtdh was slightly sma ... | 1986 | 3094925 |
| bone mineral metabolism in experimental diabetes mellitus: osteocalcin as a measure of bone remodeling. | the etiology of diabetic osteopenia has not been established. the value of serum osteocalcin (bgp) as a marker of the bone abnormalities and the possible role of the polyol pathway in diabetic osteopenia were investigated. three groups of rats were studied over 7 weeks: group d (n = 12), rats with streptozotocin (55 mg/kg)-induced diabetes given saline by gavage; group ds (n = 12), rats with streptozotocin-induced diabetes given the aldose reductase inhibitor sorbinil (25 mg/kg) daily by gavage; ... | 1988 | 3133194 |
| structural and functional studies of the effects of sympathetic nerve stimulation on rabbit submandibular salivary glands. | continuous sympathetic stimulation at 8-10 hz caused intense vasoconstriction in the gland, so stimulation was generally given in an interrupted pattern to minimize this detrimental effect on secretion. only a small increase in fluid secretion occurred; it became thick and tended to block the cannula; therefore in later experiments the main duct was not cannulated. after sympathetic stimulation there was substantial degranulation of acinar cells. however, as this was accompanied by little moveme ... | 1988 | 3165262 |
| isolation from a coastal fish of vibrio hollisae capable of producing a hemolysin similar to the thermostable direct hemolysin of vibrio parahaemolyticus. | a vibrio isolated from the intestine of a coastal fish was identified as vibrio hollisae by its biochemical characteristics. the isolate reacted with the gene probe for the thermostable direct hemolysin of vibrio parahaemolyticus. the hemolysin produced by the isolate from the fish had traits identical to those of the thermostable direct hemolysin-like hemolysin produced by a clinical strain of v. hollisae. | 1988 | 3178217 |
| excision of cutaneous tumors in the horse using histologic guidance. | a highly successful surgical technique for removing cutaneous tumors in humans was used in seven horses with cutaneous squamous cell carcinoma (n = 3) or sarcoid (n = 4). in humans, the complete underside of the tumor is evaluated histologically by processing horizontal sections from the tumor base, and orientation between the wound surface and the undersurface of the excised tumor is maintained by mapping both surfaces. the technique ensures that small foci of residual tumor can be located accu ... | 1988 | 3227634 |
| [kidney morphology in hemolytic-uremic syndrome]. | morphology of the kidneys in hemolytic-uremic syndrome is considered basing on autopsy findings obtained for 3 infants with 5-17-day history of acute renal failure. a newborn infant of 17 days developed the disease after feto-fetal hemotransfusion when macerated fetus-donor hemolysis products entered the circulation of the fetus-recipient through monochorionic placenta. the second case in an infant of 6 months was due to adtp vaccine. the last infant aged 16 months manifested the syndrome in the ... | 1988 | 3233069 |
| bacteremia caused by vibrio hollisae. | vibrio hollisae was recovered from the bloodstream of a 36-year-old man with chronic active hepatitis who was admitted to the hospital with signs of gastrointestinal illness. v. hollisae is an infrequent human pathogen associated with seafood ingestion. no etiologic link to seafood was demonstrated in this case report. | 1988 | 3343331 |
| influence of coenzyme q10 on doxorubicin uptake and metabolism by mouse myocardial cells in culture. | | 1988 | 3416371 |
| na+ modulates the k+ permeability and the membrane potential of alkalophilic bacillus. | in the absence of na+ in the medium, the membrane potential of obligately alkalophilic bacillus cells was found to be decreased by the addition of k+ to the medium, whereas k+ addition in the presence of na+ had no effect. rb+ showed essentially the same effect as k+. the decreased membrane potential was quickly restored by lowering the k+ concentration in the medium or by adding na+ or li+ to the medium. thus, in the absence of na+, the membrane potential of alkalophilic bacillus seems to be af ... | 1987 | 3663674 |
| liposome as an adjuvant for the production of estradiol antibodies. | mice immunized with liposome associated estradiol-bovine serum albumin conjugate (e2-bsa) showed a significantly higher estradiol-specific immune response than did mice injected with the free conjugate. the conjugation of estradiol with bsa is imperative, since injection of liposomes with estradiol in the lipid bilayer was found to be ineffective in inducing an immune response. entrapment of e2-bsa in neutral and positively charged liposomes was found to be a potent mode of immunostimulation, ev ... | 1987 | 3666803 |
| synthetic oligodeoxyribonucleotide probes to detect kanagawa phenomenon-positive vibrio parahaemolyticus. | synthetic oligodeoxyribonucleotide probes were used in the colony hybridization test to examine the association between the kanagawa phenomenon (kp) and the thermostable direct hemolysin gene (tdh) of vibrio parahaemolyticus. representative v. parahaemolyticus strains with a variety of kp reactions and 17 other vibrio species were examined for homology with four synthetic oligodeoxyribonucleotide probes (19 to 21 bases long) representing different regions of the tdh structural gene. under string ... | 1986 | 3711299 |
| fluid accumulation in infant mice caused by vibrio hollisae and its extracellular enterotoxin. | vibrio hollisae, a halophilic bacterium isolated from patients with diarrhea, was examined for virulence factor production. intragastric administration of 2 x 10(7) cfu per mouse elicited fluid accumulation which peaked at ca. 6 h postchallenge in infant mice. an enterotoxin which elongated chinese hamster ovary (cho) cells was detected in extracts of infected-mouse intestines and in culture fluids from various growth media. the yield of the enterotoxin was maximal beginning at the onset of the ... | 1987 | 3818087 |
| canine distemper virus does not infect oligodendrocytes in vitro. | dissociated canine brain cell cultures were infected with virulent canine distemper virus (cdv). double immunofluorescent labelling was done to simultaneously demonstrate viral antigen and specific glial cell markers. virus containing oligodendrocytes were not found at any stage of the infection. a certain proportion of the infected cells were shown to be astrocytes. it was concluded that cdv has no obvious tropism for oligodendrocytes which could explain the mechanism of demyelination in distem ... | 1985 | 3897461 |
| interactions between response stereotypy and memory strategies on the eight-arm radial maze. | three groups of water-deprived rats collected water from the ends of the 8-arm radial maze. sighted subjects, and subjects blinded either with or without pre-enucleation experience on the radial maze, all retrieved the water efficiently. most of the subjects exhibited the same response stereotypy, regularly choosing 8 adjacent arms of the maze, then stopping in the center of the maze. the strategies underlying this performance were analysed by interrupting trials and rotating the maze 180 degree ... | 1986 | 3954863 |
| detection of the thermostable direct hemolysin gene and related dna sequences in vibrio parahaemolyticus and other vibrio species by the dna colony hybridization test. | a specific gene probe for the vibrio parahaemolyticus thermostable direct hemolysin gene was constructed and used to examine the presence or absence of the thermostable direct hemolysin gene or related dna sequences in v. parahaemolyticus and other vibrios by the dna colony hybridization method. the gene probe consisted of a 406-base-pair, completely internal fragment covering 71% of the structural gene with psti linkers added to the ends. six copies of this 415-base-pair psti fragment were clon ... | 1985 | 4030087 |
| influence of dietary protein on the enzyme system synthesizing ascorbic acid in the rat. | | 1968 | 4234667 |
| the response of the pituitary-adrenal system to a stressful stimulus: the effect of conditioning and pentobarbitone treatment. | | 1965 | 4284301 |
| virus infections of horses at newmarket, 1972 and 1973. | | 1974 | 4375339 |
| temperature changes following microinjection of histamine into the thermoregulatory centers of the rat. | | 1970 | 4391628 |
| anion effects on pyruvate kinase. | rabbit muscle pyruvate kinase is shown not to be activated by several anions (acetate, chloride, lactate, nitrate and sulphate) when tested as their tetramethylammonium salts. this contradicts the conclusions of focant & watts (1973). | 1974 | 4464855 |
| biogenesis of mitochondria. xxv. studies on the mitochondrial genomes of petite mutants of yeast using ethidium bromide as a probe. | | 1973 | 4583801 |
| cationic environment and dynamics of insulin secretion. iv. effect of ouabain. | | 1974 | 4615999 |
| cationic environment and dynamics of insulin secretion. iv. effect of ouabain. | | 1974 | 4615999 |
| illness caused by vibrio damsela and vibrio hollisae. | vibrio damsela was isolated from six wound infections in otherwise healthy persons. in five of the six cases the wounds were known to have been exposed to salt or brackish water at the time of the injury. vibrio hollisae was isolated from an index stool culture in nine cases in which no other enteric pathogen was identified. all nine patients had diarrhoea and abdominal pain; one patient had bloody diarrhoea. six of the nine patients were known to have eaten raw seafood in the five days before t ... | 1982 | 6123029 |
| identification of vibrio hollisae sp. nov. from patients with diarrhea. | the name vibrio hollisae (synonym = special bacteriology group ef-13) is proposed for a new group of 16 strains that occurred in stool cultures of patients with diarrhea. v. hollisae is a small gram-negative rod, which is motile with a single polar flagellum. no lateral or peritrichous flagella were observed, even when it was grown on a solid medium. sodium chloride is required for growth, so v. hollisae is a halophilic vibrio. strains were positive (36 degrees c, 24 or 48 h) for oxidase (kovacs ... | 1982 | 7076812 |
| adherence to and invasion of tissue culture cells by vibrio hollisae. | the adherence to and invasion of cultured epithelial cells by vibrio hollisae were examined by quantitative studies and by light, fluorescent, and electron microscopy. condensed actin was observed around clustered adherent and intracellular bacteria. bacteria multiplied intracellularly. inhibitor studies indicated that internalization occurred by an integrated pleiotropic process involving eukaryotic and prokaryotic protein syntheses, microfilaments, microtubules, and receptor-mediated endocytos ... | 1995 | 7591167 |
| purification and characterization of a chinese hamster ovary cell elongation factor of vibrio hollisae. | the halophilic bacterium vibrio hollisae, isolated from patients with diarrhea, produces an extracellular toxin which elongates chinese hamster ovary (cho) cells. we purified this toxin to homogeneity by sequential ammonium sulfate precipitation, gel filtration with sephacryl s-200, hydrophobic interaction chromatography with phenyl-sepharose cl-4b, ion-exchange chromatography with deae-sephadex a-50, and affinity chromatography. the toxin is heat labile and sensitive to proteases, with an isoel ... | 1995 | 7790052 |
| identification of vibrio hollisae associated with severe gastroenteritis after consumption of raw oysters. | vibrio hollisae was recovered from the stool culture of a 40-year-old female hospitalized for severe abdominal cramping, vomiting, fever, and watery diarrhea. she had consumed two dozen raw oysters 5 days prior. there was only a single colony on thiosulfate-citrate-bile salts-sucrose-agar, and definitive identification required conventional test media with 1% nacl. | 1994 | 7929777 |
| severe gastroenteritis associated with vibrio hollisae infection: report of two cases and review. | vibrio hollisae, one of the more recently described halophilic vibrio species, is infrequently associated with gastrointestinal disease and only rarely recovered from individuals presenting with gram-negative sepsis. in this report we describe two cases of severe gastrointestinal disease associated with v. hollisae in otherwise healthy individuals. in one of these individuals, severe epigastric pain was apparently associated with signs of pseudoappendicitis, necessitating exploratory surgery. in ... | 1994 | 8011809 |
| identification of the siderophores from vibrio hollisae and vibrio mimicus as aerobactin. | hydroxamate siderophores were purified from low-iron cultures of vibrio hollisae atcc 33564 and vibrio mimicus atcc 33653. by a combination of 1h and 13c nmr spectroscopy, fast atom bombardment mass spectrometry, and compositional analysis, both of the siderophores were identified as aerobactin, a citrate-based dihydroxamate siderophore, which is highly prevalent in species of the family enterobacteriaceae. four other clinical strains belonging to these species also produced aerobactin. in respo ... | 1994 | 8013878 |
| the thermostable direct hemolysin gene (tdh) of vibrio hollisae is dissimilar in prevalence to and phylogenetically distant from the tdh genes of other vibrios: implications in the horizontal transfer of the tdh gene. | vibrio hollisae strains isolated recently from patients in various locations were examined for the presence of the thermostable direct hemolysin gene (tdh) using nucleic acid hybridization and polymerase chain reaction assays. the results were consistent with the previous finding that all strains of v. hollisae carry the tdh gene. in contrast, the tdh gene has been detected in a minority of strains for other vibrio species (v. parahaemolyticus, v. cholerae non-o1, and v. mimicus). detailed phylo ... | 1996 | 8871530 |
| first european case of gastroenteritis and bacteremia due to vibrio hollisae. | vibrio hollisae is a pathogenic vibrio species known to cause gastroenteritis in humans after the consumption of shellfish. all cases of infection reported previously were restricted to the atlantic and pacific coasts of the united states. a case of gastroenteritis and bacteremia in a previously healthy 76-year-old man who ate cockles from the quiberon bay in brittany, france, is described. this is the first report of vibrio hollisae infection in europe. | 1996 | 8997558 |
| identification of major antigenic proteins of pasteurella piscicida. | two different antigenic protein-coding clones (ppa1 and ppa2) were isolated using anti-pasteurella piscicida rabbit serum from a genomic dna library of p. piscicida strain kp9038. the ppa1 and ppa2 expressed 7 kda and 45 kda proteins in escherichia coli, respectively, and the molecular sizes of these expressed proteins are the same as these of the major antigenic proteins of p. piscicida. ppa1 encodes a protein of 83 amino acids residues, which is similar to the bacterial lipoprotein. comparison ... | 1997 | 9441863 |
| response of pathogenic vibrio species to high hydrostatic pressure. | vibrio parahaemolyticus atcc 17802, vibrio vulnificus atcc 27562, vibrio cholerae o:1 atcc 14035, vibrio cholerae non-o:1 atcc 14547, vibrio hollisae atcc 33564, and vibrio mimicus atcc 33653 were treated with 200 to 300 mpa for 5 to 15 min at 25 degrees c. high hydrostatic pressure inactivated all strains of pathogenic vibrio without triggering a viable but nonculturable (vbnc) state; however, cells already existing in a vbnc state appeared to possess greater pressure resistance. | 1999 | 10347079 |
| analysis of gyrb and toxr gene sequences of vibrio hollisae and development of gyrb- and toxr-targeted pcr methods for isolation of v. hollisae from the environment and its identification. | isolation of vibrio hollisae strains, particularly from the environment, is rare. this may be due, in part, to the difficulty encountered when using conventional biochemical tests to identify the microorganism. in this study, we evaluated whether two particular genes may be useful for the identification of v. hollisae. the two genes are presumed to be conserved among the bacterial species (gyrb) or among the species of the genus vibrio (toxr). a portion of the gyrb sequence of v. hollisae was cl ... | 2000 | 10919814 |
| vibrio calviensis sp. nov., a halophilic, facultatively oligotrophic 0.2 microm-fiiterabie marine bacterium. | a gram-negative, facultatively anaerobic, straight to slightly curved rod-shaped bacterium (re35f/12t) sensitive to vibriostatic agent o/129 was previously isolated from sea water (western mediterranean sea, bay of calvi, corsica, france) by 0.2 microm-membrane filtration. strain re35/f12t (= cip 107077t = dsm 14347t) was facultatively oligotrophic, halophilic, required na+ for growth and produced acid but no gas from d-glucose under anaerobic conditions. comparative 165 rrna gene-sequence analy ... | 2002 | 11931167 |
| enterovibrio norvegicus gen. nov., sp. nov., isolated from the gut of turbot (scophthalmus maximus) larvae: a new member of the family vibrionaceae. | twenty-two isolates originating from the gut of healthy cultured turbot larvae in norway were investigated using a polyphasic approach. amplified fragment length polymorphism fingerprinting analysis showed that the isolates have typical patterns and form two main groups. phylogenetic analysis revealed that the isolates belong to the gamma-proteobacteria, with vibrio hollisae as their closest neighbour. dna-dna hybridization, chemotaxonomic and phenotypic analyses further proved that these isolat ... | 2002 | 12508862 |
| reclassification of vibrio hollisae as grimontia hollisae gen. nov., comb. nov. | the taxonomic positions of three representative strains of vibrio hollisae (lmg 17719(t), lmg 21416 and lmg 21538) were investigated by means of 16s rdna sequences and phenotypic data. v. hollisae strains (genbank/embl accession nos aj514909-aj514911) shared 99.5 % 16s rdna sequence similarity, but had only 94.6 % similarity to their closest phylogenetic neighbour, enterovibrio norvegicus. 16s rdna sequence similarity of v. hollisae and vibrio cholerae was only 91 %. these results suggest that v ... | 2003 | 13130058 |
| use of reca as an alternative phylogenetic marker in the family vibrionaceae. | this study analysed the usefulness of reca gene sequences as an alternative phylogenetic and/or identification marker for vibrios. the reca sequences suggest that the genus vibrio is polyphyletic. the high heterogeneity observed within vibrios was congruent with former polyphasic taxonomic studies on this group. photobacterium species clustered together and apparently nested within vibrios, while grimontia hollisae was apart from other vibrios. within the vibrios, vibrio cholerae and vibrio mimi ... | 2004 | 15143042 |
| biodiversity of vibrios. | vibrios are ubiquitous and abundant in the aquatic environment. a high abundance of vibrios is also detected in tissues and/or organs of various marine algae and animals, e.g., abalones, bivalves, corals, fish, shrimp, sponges, squid, and zooplankton. vibrios harbour a wealth of diverse genomes as revealed by different genomic techniques including amplified fragment length polymorphism, multilocus sequence typing, repetetive extragenic palindrome pcr, ribotyping, and whole-genome sequencing. the ... | 2004 | 15353563 |
| rapid phenotypic characterization of vibrio isolates by pyrolysis metastable atom bombardment mass spectrometry. | pyrolysis mass spectrometry was investigated for rapid characterization of food-borne bacterial pathogens. nine isolates of vibrio parahaemolyticus and one isolate each of vibrio fluvialis, vibrio hollisae, and vibrio vulnificus were analyzed. pyrolysis mass spectra, generated via an alternative ionization method, metastable atom bombardment, were subject to principal component-discriminant analysis. the spectral patterns were used to distinguish vibrio isolates differing in species, serotype an ... | 2005 | 16096691 |
| identification and transcriptional organization of aerobactin transport and biosynthesis cluster genes of vibrio hollisae. | we had previously reported that vibrio hollisae produces aerobactin in response to iron starvation. in the present study, we identified in v. hollisae atcc33564 the aerobactin system cluster which consists of eight genes, hatcdb, iucabcd and iuta. the hatcdb genes encode proteins homologous to components of bacterial atp binding cassette transport systems for ferric aerobactin. the iucabcd and iuta orthologs code for aerobactin biosynthesis enzymes and the ferric aerobactin receptor, respectivel ... | 2006 | 16809025 |
| rugosity in grimontia hollisae. | grimontia hollisae, formerly vibrio hollisae, produces both smooth and rugose colonial variants. the rugose colony phenotype is characterized by wrinkled colonies producing copious amounts of exopolysaccharide. cells from a rugose colony grown at 30 degrees c form rugose colonies, while the same cells grown at 37 degrees c form smooth colonies, which are characterized by a nonwrinkled, uncrannied appearance. stress response studies revealed that after exposure to bleach for 30 min, rugose surviv ... | 2007 | 17189437 |
| severe gastroenteritis and hypovolemic shock caused by grimontia (vibrio) hollisae infection. | vibrio hollisae is a halophilic species that was recently reclassified as grimontia hollisae. this organism is known to cause moderate to severe cases of gastroenteritis. we report a case of an individual who suffered a more severe form of this disease, presenting with profound hypotension and acute renal failure, secondary to hypovolemic shock. | 2007 | 17704283 |
| genes encoding the vibrio harveyi haemolysin (vhh)/thermolabile haemolysin (tlh) are widespread in vibrios. | v. harveyi vhh haemolysin, which shows high homology to the tlh haemolysin (the identities of their deduced amino acid sequences are up to 85.6%), is a putative virulence factor to marine cultured fish. a vhh probe, which is specific to v. harveyi vhha haemolysin gene, was used to screen ecor i digests of total dna from 57 vibrio strains, including 26 vibrio type strains, 20 v. harveyi isolates and 11 v. parahaemolyticus isolates. as a result, 1 strong hybridisation band was detected in 13 type ... | 2007 | 18062266 |
| grimontia hollisae, a potential agent of gastroenteritis and bacteraemia in the mediterranean area. | vibrio hollisae was first described in 1982 as an agent of diarrhoea and was reclassified in 2003 into a novel genus as grimontia hollisae. we report the first case of g. hollisae bacteraemia in the mediterranean area, in an 81-year-old man with a severe gastroenteritis and hepatitis following the consumption of raw oysters. the incidence of this micro-organism as an agent of gastroenteritis may be underestimated because it may not be detected using routine culture conditions. | 2009 | 19089475 |
| vibrios as causal agents of zoonoses. | vibrios are gram-negative rod-shaped bacteria that are widespread in the coastal and estuarine environments. some species, e.g. vibrio anguillarum and v. tapetis, comprise serious pathogens of aquatic vertebrates or invertebrates. other groups, including grimontia (=vibrio) hollisae, photobacterium (=vibrio) damselae subsp. damselae, v. alginolyticus, v. harveyi (=v. carchariae), v. cholerae, v. fluvialis, v. furnissii, v. metschnikovii, v. mimicus, v. parahaemolyticus and v. vulnificus, may cau ... | 2010 | 19342185 |
| rapid and specific detection of the thermostable direct hemolysin gene in vibrio parahaemolyticus by loop-mediated isothermal amplification. | several investigators have reported that thermostable direct hemolysin (tdh) and tdh-related hemolysin are important virulence factors of vibrio parahaemolyticus, but it has been difficult to detect these factors rapidly in seafood and other environmental samples. a novel nucleic acid amplification method, termed the loop-mediated isothermal amplification (lamp), which amplifies dna with high specificity and rapidity under isothermal conditions, was applied. in this study, we designed tdh gene-s ... | 2009 | 19435222 |
| rna colony blot hybridization method for enumeration of culturable vibrio cholerae and vibrio mimicus bacteria. | a species-specific rna colony blot hybridization protocol was developed for enumeration of culturable vibrio cholerae and vibrio mimicus bacteria in environmental water samples. bacterial colonies on selective or nonselective plates were lysed by sodium dodecyl sulfate, and the lysates were immobilized on nylon membranes. a fluorescently labeled oligonucleotide probe targeting a phylogenetic signature sequence of 16s rrna of v. cholerae and v. mimicus was hybridized to rrna molecules immobilized ... | 2009 | 19561182 |
| development of a loop-mediated isothermal amplification assay for sensitive and rapid detection of the tdh and trh genes of vibrio parahaemolyticus and related vibrio species. | thermostable direct hemolysin (tdh) and tdh-related hemolysin (trh) are the major virulence determinants of vibrio parahaemolyticus. trh is further differentiated into trh1 and trh2 on the basis of genetic and phenotypic differences. we developed a novel and highly specific loop-mediated isothermal amplification (lamp) assay for sensitive and rapid detection of the tdh, trh1, and trh2 genes of v. parahaemolyticus. the lamp assay was designed for both combined and individual detection of the tdh, ... | 2010 | 19966027 |
| rapid identification and characterization of vibrio species using whole-cell maldi-tof mass spectrometry. | vibrio identification by means of traditional microbiological methods is time consuming because of the many biochemical tests that have to be performed to distinguish closely related species. this work aimed at evaluating the use of maldi-tof mass spectrometry for the rapid identification of vibrio (v.) spp. as an advantageous application to rapidly discriminate the most important vibrio spp. and distinguish vibrio spp. from closely related bacterial species like photobacterium damselae and grim ... | 2010 | 20059616 |
| purification, crystallization and preliminary x-ray analysis of a thermostable direct haemolysin from grimontia hollisae. | vibrio hollisae, a halophilic species recently reclassified as grimontia hollisae, is a causative agent of gastroenteritis and septicaemia. one important pathogenic vibrio factor, thermostable direct haemolysin (tdh), has been purified and crystallized in two crystal forms using the vapour-diffusion method. the crystals belonged to an orthorhombic space group, with unit-cell parameters a = 104.8, b = 112.4, c = 61.3 å and a = 122.9, b = 123.3, c = 89.8 å. the crystals contained either four or ei ... | 2011 | 21301091 |
| site-directed mutations of thermostable direct hemolysin from grimontia hollisae alter its arrhenius effect and biophysical properties. | recombinant thermostable direct hemolysin from grimontia hollisae (gh-rtdh) exhibits paradoxical arrhenius effect, where the hemolytic activity is inactivated by heating at 60 °c but is reactivated by additional heating above 80 °c. this study investigated individual or collective mutational effect of tyr53, thr59, and ser63 positions of gh-rtdh on hemolytic activity, arrhenius effect, and biophysical properties. in contrast to the gh-rtdh wild-type (gh-rtdh(wt)) protein, a 2-fold decrease of he ... | 2011 | 21494434 |
| cloning of a novel collagenase gene from the gram-negative bacterium grimontia (vibrio) hollisae 1706b and its efficient expression in brevibacillus choshinensis. | the collagenase gene was cloned from grimontia (vibrio) hollisae 1706b, and its complete nucleotide sequence was determined. nucleotide sequencing showed that the open reading frame was 2,301 bp in length and encoded an 84-kda protein of 767 amino acid residues. the deduced amino acid sequence contains a putative signal sequence and a zinc metalloprotease consensus sequence, the hexxh motif. g. hollisae collagenase showed 60 and 59% amino acid sequence identities to vibrio parahaemolyticus and v ... | 2011 | 21515782 |
| Detection and characterization of pathogenic vibrios in shellfish by a Ligation Detection Reaction-Universal Array approach. | Vibrios are a group of major foodborne pathogens widely distributed in marine environment. Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus are the pathogenic species of Vibrio that pose the greatest threat to human health. However, other vibrios, e.g. Vibrio alginolyticus, Vibrio mimicus and Grimontia hollisae, apparently less relevant in the group of foodborne pathogens, have been sporadically found in outbreaks. For seafood safety and economic purposes, a rapid and powerful met ... | 2011 | 22177227 |
| fetal heart rate during a maternal grand mal epileptic seizure. | although maternal ingestion of antiepileptic drugs is strongly suspected of causing congenital defects, particularly oral clefts, the effect of epilepsy itself or a combined effect of drug intake and epilepsy have not been excluded as etiological factors. very little is known about fetal oxygenation during a maternal grand mal epileptic seizure. we describe two cases in which fetal heart rate was recorded during a maternal epileptic seizure during labor. the first fetus became clearly asphyctic ... | 1998 | 106102 |
| mechanism of action of beta-blockers in hypertension. | | 2001 | 14643 |
| pelvic displacement of a cardiac pacemaker following trauma. a case report. | the rate migration of an implanted cardiac pacemaker from the subrectus area anterior to the peritoneum to the pelvis following a motor vehicle accident is reported. discovery of dislodgement was made by observing a marked increase of pacemaker impulse amplitude in the three standard limb leads as measured on an oscilloscope. | 2003 | 152472 |
| male transsexualism in england: sixteen cases with surgical intervention. | the syndrome of transsexualism is now regarded as a dinstinct entity separate from transvestism and homosexuality (benjamin, 1966; green and money, 1969). it refers to those patients who since childhood have been convinced of their wrong sex assignment and who wish to attain the sexual characteristics of the opposite sex and to live as a member of that sex. the present report is an initial one referring to the first group of transsexual patients studied at a postgraduate teaching hospital by a m ... | 2005 | 1169047 |
| effect of a mixture of cryoprotectants in attaining liquid nitrogen survival of callus cultures of a tropical plant. | | 2005 | 544179 |
| anti-rh globulin in massachusetts. | | 2014 | 4178642 |
| [the development of osseointegrated implants]. | | 2007 | 1932767 |
| [artero-venous angiodysplasia at the splenic hylum: contribution to the knowledge of pre-hepatic artero-venous angiodysplasias with portal hypertension]. | | 2014 | 4080430 |
| [new trematode genera from fishes of the southern atlantic]. | two new genera of the lepocreadiid trematodes, gibsonia gen. n., (type species--g. hastata sp. n.) from macrourus carinatus günther, 1878 (macrouridae), collected in the falkland islands area, and muraenolepitrema gen. n. (type species--m. magnatestis sp. n.) from muraenolepis microps lönnberg, 1905 (muraenolepidae), collected in the south georgia island area, are described. gibsonia gen. n. differs from all known genera of the family lepocreadiidae by unusual body shape divided into 2 parts, su ... | 2011 | 3226778 |
| [x-ray computed tomography in venous pathology]. | after presenting the tomodensitometric semiology of venous thromboses in various territories, the authors define its indications. it is essentially in deep territories, head, chest and abdomen that the ct-scan is used to study and diagnose unrecognized thromboses as well as determine the venous repercussion of various neighboring ailments. the respective place of the ct-scan, ultrasonography and various phlebographies is analyzed. | 2011 | 3043474 |
| noninvasively diagnosed peripheral arterial disease as a predictor of mortality: results from a prospective study. | intermittent claudication has been reported in previous studies to approximately double the risk of subsequent mortality. however, a history of claudication is often present in the absence of significant peripheral arterial disease (pad) and absent in the presence of pad. for this reason we evaluated the association between large-vessel and small-vessel pad, measured by highly reliable and valid noninvasive tests, and mortality in 567 older subjects from a defined population followed-up for an a ... | 2014 | 4028377 |
| the mesencephalic exterolateral posterior nucleus of the mormyrid fish bryenomyrus niger: efferent connections studied by the hrp method. | | 1999 | 91414 |
| hypersomatotropism and acanthosis nigricans in two brothers. | | 2016 | 4843205 |
| [newly designed ultraviolet light monochromator]. | | 2015 | 4542243 |
| initial management of cutaneous malignant melanoma. | appropriate initial surgical management of primary cutaneous malignant melanoma is important since no effective treatment exists once the tumour metastasizes. the author reviews methods of initial treatment, excisional biopsy with adequate margins being the most common. he also reports results from a questionnaire survey on the type of microstaging used at hospitals across canada. two thirds of the pathologists who responded to the questionnaire preferred to use both clark's and breslow's method ... | 2013 | 3719498 |
| [left ventricular wall thickness in hypertrophic cardiomyopathy: comparisons of measurements by magnetic resonance imaging, cineangiography, and echocardiography]. | to evaluate the usefulness of magnetic resonance imaging (mri) in measuring regional wall thickness of the left ventricle, 11 patients with hypertrophic cardiomyopathy (hcm) were imaged. the measurements were compared with those obtained by two-dimensional echocardiography (2d-echo) and cineangiography. regional wall thickness was ascertained at six locations including the base, middle, and apex of the interventricular septum (ivs) and left ventricular posterior wall (lvpw) from the two selected ... | 2012 | 3267712 |
| isotachophoresis of csf proteins in gel tubes especially gammaglobulins. an analytical and preparative technique for high-separation of csf proteins. | an isotachophoretic method using polyacrylamide gel (pag-itp) in a simple disc electrophoretic equipment with plastic tubes containing the gels, was elaborated and especially designed for studying the gammaglobulins in csf and serum from control subjects and patients with neurological disorders, especially known or probable ms. the device and the itp system used, including leading and terminating electrolytes and spacer substances, dividing the gammaglobulins in a reproducible way, are described ... | 2001 | 92548 |
| [medical management of tachycardias (author's transl)]. | guidelines for a step-wise plan of treatment of tachycardias have been compiled based on clinical empirical experience and with the aid of surface electrocardiograms, intracardial electrograms and stimulation techniques. the plan is primarily with the aid of surface electrocardiograms, intracardial electrograms and stimulation techniques. the plan is primarily oriented with respect to the antiarrhythmic efficacy, the adverse reactions and the practicability of the respective agents. any type of ... | 2001 | 93075 |
| a simple centrifuge column for desalting protein solutions. | | 2005 | 543535 |
| health checks on patients 75 years and over in nottinghamshire after the new gp contract. | to investigate annual health checks for patients of 75 years and over required by the 1990 contract for general practitioners. | 2006 | 1393076 |
| [cytodiagnosis using fine needle aspiration in otorhinolaryngologic pathology]. | | 2012 | 3334298 |
| reference ranges and ideal patient values for blood cholesterol. can there be reconciliation? | reference ranges for blood cholesterol levels reflect the nationwide epidemic of "silent" hypercholesterolemia. as such, reference ranges for cholesterol levels without accompanying designation of levels associated with increased coronary risk are misleading and falsely comforting to physicians and patients. in a recent continent-wide study of 108 academically based clinical laboratories, highly variable reference ranges were observed, the ranges having been derived from a vast assortment of sou ... | 2013 | 3754717 |
| a dental family's impact on the community: the many contributions of john and vada somerville. | | 2013 | 3910146 |
| diaphragmatic movement using ultrasound during spontaneous and mechanical ventilation: effect of tidal volume. | using ultrasound (us) the effect of various tidal volumes on the movement of ventral, dome and dorsal parts of the right hemidiaphragm was studied, both during spontaneous and mechanical ventilation. six healthy non-medicated volunteers who were in the supine position breathed spontaneously shallowly (tidal volume (vt) being 400 ml) (sb), and deeply (vt 1000 ml) (sb-deep). in addition, they were mechanically ventilated with intermittent positive pressure ventilation at three different vt's: 500 ... | 2006 | 1449048 |
| [effectiveness of the comprehensive treatment by rhonidase electrophoresis and tbilisi sulfide baths of elderly patients suffering from osteoarthrosis deformans]. | | 2003 | 154769 |