interaction of thermostable direct hemolysin of vibrio parahaemolyticus with human erythrocytes.the interaction between thermostable direct hemolysin produced by vibrio parahaemolyticus wp-1 and human erythrocytes was studied. the lysis of human erythrocytes by the hemolysin was dependent of temperature and no hemolysis occurred at low temperature (0-4 c), but the hemolysin was adsorbed on human erythrocytes even at low temperature. no hemolysis was observed when antihemolysin antiserum was mixed with the hemolysin and human erythrocytes at zero time. on the other hand, lysis of the cells ...19753160
suitability of some enrichment broths and diluents for enumerating cold- and heat-stressed vibrio parahaemolyticus.vibrio parahaemolyticus cells were injured by chilling and heating, and their recovery was tested in glucose-salt-teepol broth (gstb), tryptic soy broth containing 7% nacl (tsbs), horie - arabinose - ethyl violet broth (haeb), and water blue - alizarin yellow broth (wbay). exponential phase cells were more sensitive to cold shock than were stationary phase cells. exposure of chill-injured v. parahaemolyticus to gstb and tsbs resulted in 70 to 80% death; about 70% lethality was noted for heat-in ...197717461
cyclic guanosine-3',5'-monophosphate--mediator of the action of the hormaone erythropoietin. 197718327
partial characterization of a beta-lactamase from vibrio parahaemolyticus by a new automated microiodometric technique.a number of characteristics were determined with a new automated method for a partially purified beta-lactamase from vibrio parahaemolyticus. the enzyme had a molecular weight of 28,000 by gel filtration, a ph optimum between 6.5 and 7.0, and a temperature optimum at 36 degrees c. with penicillin g as the substrate, the k(m) value for the beta-lactamase was 54.4 mum. the beta-lactamase was inhibited by cloxacillin but not by p-chloromercuribenzoate. the enzyme was similar but not identical to be ...197825616
purification of lysophospholipase of vibrio parahaemolyticus and its properties.lysophospholipase ec was solubilized from the cells of vibrio parahaemolyticus with triton x-100 and purified by the following procedure; precipitation with ammonium sulfate, acid treatment and ion exchange column chromatography using deae-cellulose, deae-sephadex a-50, and cm-cellulose, successively. the purified preparation was shown to be homogeneous by polyacrylamide gel disk electrophoresis. the isoelectric point of the enzyme was found to be around ph 3.64 by isoelectric focusing e ...197826676
a new method for testing the kanagawa phenomenon of vibrio parahaemolyticus with a liquid medium. 197935733
effects of ph of the medium on flagellation of vibrio parahaemolyticus.formation of the lateral flagella by vibrio parahaemolyticus was inhibited under an alkaline condition of the medium. however, flagellation of the polar monotrichous flagellum was not affected in the same condition. flagellation of the lateral flagella depended on the ph of the medium.197939500
antigenicity of lateral flagella of vibrio parahaemolyticus: existence of two distinct antigenic determinants in a flagellum.cells of vibrio parahaemolyticus treated with formalin agglutinated with anti-lateral flagella antiserum. on the basis of agglutination tests, antigens of lateral flagella were divided into three groups. hl1, hl2, and hl3. however, in gel diffusion tests, flagellins prepared from strains belonging to different groups showed a common antigenicity. it is assumed that these results are due to the existence of two distinct antigenic determinants in the lateral flagella. one of them exists on the sur ...197769612
possible presence of a genus-specific antigen in vibrios. 197773372
leprosy's footprints in bone-marrow histiocytes. 197985259
in vitro synthesis of infectious transforming dna by the avian sarcoma virus reverse transcriptase.infectious dna molecules, capable of transforming chicken embryo fibroblasts, can be synthesized by the rous sarcoma virus-associated reverse transcriptase in vitro. the optimal enzymatic conditions employed for infectious dna synthesis also facilitate maximum synthesis of genome length dna. analysis of the dna product synthesized by detergent-disrupted rous sarcoma virus under these conditions indicates that dna complementary to viral rna (minus-strand dna) is genome length in size, whereas dna ...197985719
immunochemical properties of oligosaccharide-protein conjugates with klebsiella-k2 specificity. i. specificity and crossreactivity of anti-conjugate versus anti-bacterial antibodies. 197986152
the puzzle of "urethral syndrome": a possible answer?82 strains of slow-growing, co2-dependent, gram-positive organisms have been isolated from the urine of 9 male and 73 female patients. 100% of the men and 93% of the women had urinary symptoms at the time of isolation, and 66% of the specimens showed pyuria. these organisms might account for the urinary symptoms of some patients previously diagnosed as having "urethral syndrome". preliminary data show that, if appropriate antibacterial treatment is given, most of these patients become symptom-fr ...197986778
the cerebellar projection from the lateral reticular nucleus as studied with retrograde transport of horseradish peroxidase.the cerebellar projection from the lateral reticular nucleus (nrl) was studied in cats by means of retrograde axonal transport of horseradish peroxidase (the projection to the paramedian lobule was not included, see brodal, 1975, for afferents to this cortical region). the entire cerebellar cortex and all cerebellar nuclei receive fibres from the nrl. the strongest connection is with the anterior lobe and lobulus viiib of the posterior lobe vermis. as concerns the anterior lobe the observations ...197988191
lipopolysaccharide-derived serotype polysaccharides from neisseria meningitidis group b.three immunologically distinct types of polysaccharides have been isolated by diethylaminoethyl (deae) chromatography from the lipopolysaccharide extracts of group b neisseria meningitidis. all types contain a set of common determinants, as well as distinct ones; all of these determinants are detectable by either immunodiffusion or enzyme-linked immunosorbent assay (elisa). the polysaccharides elute from a sepharose 4b column in the range of 2-3 x 10(5) daltons and have isoelectric points from 4 ...197988491
techniques for evaluating humoral and cell-mediated immunity in mule deer fawns (odocoileus hemionus).twenty mule deer fawns (odocoileus hemionus) were removed from their dams 48 h after birth, and hand-reared. methods for monitoring their immune capability are described. passive humoral immunity was determined by serum protein electrophoresis. active humoral immunity following clostridium toxoid vaccination was determined by immunodiffusion. cell-mediated immunity was assayed using contact sensitization to 1-nitro, 2,4-dichlorobenzene (dncb).197988530
adjvant treatment of tongue and floor of the mouth cancers.since january 1974, 95 patients with anterior tongue and floor of the mouth cancers were included in a randomized trial. after stratification according to staging and initial treatment, one-third of the patients received chemotherapy for 2 years (methotrexate 400 mg followed by citrovorum factor 100 mg + bleomycin 60 mg/week, during the first 15 weeks), one-third of the patients received immunotherapy with weekly c. parvum injections during 2 years, while the remaining third did not receive any ...197888754
characterisation of the reaginic response to sulphonamides in mice.reaginic responses were induced in immunised mice with either 4-sulphanilamidobenzoic acid (4-saba) or sulphamethoxazole (smx) coupled to chicken gamma-globulin (cgg). the former was coupled through the carboxylic group of benzoic acid and the latter through the diazo derivative of the sulphanilamide group. the specificity of the reaginic responses obtained was assessed in each case by inhibition of passive cutaneous anaphylaxis in the rat. immunisation with 4-saba-cgg resulted in antibodies whi ...197989094
antigenic relationships between polypeptides derived from plantar and hand wart viruses.guinea pigs immunized with polypeptides derived from plantar and hand wart viruses developed both humoral and cell mediated immunity. the specificity of the antiserum was determined by indirect immunofluorescence (if) tests and cellular immunity by intradermal tests. while whole plantar and hand wart virus particles (pv and hv) appeared to be immunologically analogous, they had different polypeptide patterns as shown by analysis on acrylamide slab gels. in particular, the p2 polypeptide (major v ...197990120
the intra-nucleolar localization of amplified rdna in xenopus laevis oocytes.a fluorescent feulgen-stain was adapted in order to demonstrate dna-containing structures inside the amplified nucleoli of xenopus laevis. at all stages of oogenesis this method reveals granules or complex structures of dna in each nucleolus. the micronucleoli which do not stain with this method and which do not reveal an internal structure in low molarity saline, unlike real nucleoli are considered as nucleolus-like bodies. the dna-containing structures in the nucleoli can be composed of one or ...197990588
head lice and hygiene. 197991008
the hepatitis b virus and its dna polymerase: the prototype three-d virus.the hepatitis b virus (hbv), the causal agent of serum hepatitis, has a diameter of 42 nm and is comprised of an outer surface coat and a 27 nm core. a unique dna-dependent dna polymerase is associated with the core of the virus. the core also houses a circular dna that contains both double-stranded and single-stranded regions. in the endogenous reaction, the dna polymerase repairs the single-stranded gaps of the viral dna. the surface protein of the virus, called hepatitis b surface antigen, co ...197991092
monolayer absorption of human cytotoxic t cells: evidence for clonality. 197991211
human gastrointestinal cancer grown in nude mice used to test new chemotherapeutic agents. 197991567
behavior of a surface antigenic determinant of lateral flagella of vibrio parahaemolyticus.the behavior of a surface antigenic determinant (sa) of lateral flagella of vibrio parahaemolyticus was studied. sa, located on the surface of flagellar filaments, is responsible for h-agglutination and specific for serotype. results of h-agglutination inhibition tests demonstrated that sa could not be detected on the flagellin molecule when the flagellar filaments were dissociated to flagellin monomers by heating or treatment with urea, sodium dodecyl sulfate, hcl, or acetone, although sa could ...197991584
generation of a fibroblast chemotactic factor in serum by activation of complement.when serum complement is activated by either the classical or alternative pathways, a factor with an apparent 80,000 mol wt is generated that is chemotactic for human dermal fibroblasts. the origin of this serum-derived chemotactic factor (sdcf) is not known; however, it may be a cleavage product from c5 because it is inactivated by monospecific antiserum to human c5, and it is not generated when the complement system is activated in human serum deficient in c5. sdcf is not chemotactic for human ...197991624
variation in orbiviruses. 197991662
response to immunological or cholinergic stimulation of isolated mast cells from man, guinea pig and rat. 197991954
[astroprotein in cerebrospinal fluid following experimental brain injury (author's transl)]. 197991995
[cutaneous leishmaniasis. alcoy: an endemic zone]. 197992178
effect of fat structure on total, on chylomicron, and on high-density lipoprotein cholesterol in the absorptive state in man [proceedings]. 197992248
[influence of a dialyzable serum factor on the activity of beta-galactosidase from human liver (proceedings)]. 197992267
occurrence of isoprene lipids in bovine thyroid [proceedings]. 197992282
suramin: a potent inhibitor of the reverse transcriptase of rna tumor viruses.suramin--a well-known antitrypanosomal agent--was found to exert a strong inhibitory effect on the rna-directed dna polymerase (reverse transcriptase) activity of several oncornaviruses such as moloney murine leukemia virus, murine rauscher leukemia viruses, moloney murine sarcoma virus and avian myeloblastosis virus. inhibition of enzyme activity was obtained with both endogenous viral rna and (a)n . oligo(dt) as the template-primer. suramin effected a 50% inhibition of the reverse transcriptas ...197992362
characterization of estrogen binding in the developing rat testis. ontogeny of the testicular cytoplasmic estrogen receptor.the properties and physical characteristics of a steroid binding component present in the immature (7 to 35 day) rat were investigated and found to be different from those of the 17 beta-estradiol receptor in the mature rat testis. these properties include a binding capacity of 483 fmol estradiol/mg protein, a ka at equilibrium of 4.23 x 10(7)m-1, and broad steroid specificity as shown by interaction with several steroids; no binding was observed with diethylstilbestrol. the component, found in ...197992832
prostate-specific acid phosphatase: purification and specific antibody production in rabbits.demonstration of prostate-specific acid phosphatase by immunologic methods in tissue sections or in plasma necessitates a monospecific antiserum. this is produced by immunizing rabbits with pure prostate-specific acid phosphatase antigen, prepared from seminal fluid. the ejaculate is centrifuged, dialyzed against a citrate buffer, ph 4.8, and centrifuged again. the supernatant is brought onto a sephacryl s-200 superfine column and eluted with the same buffer. prostate-specific acid phosphatase-p ...197992890
analysis of vibrio parahaemolyticus soluble antigens by employing passive hemagglutination.the passive hemagglutination assay was explored as a sensitive test of immunological reactivity of endotoxin and other antigens prepared from selected strains of vibrio parahaemolyticus. the tannic acid procedure for passive hemagglutination, commonly used with protein antigens, was the only procedure yielding good results with v. parahaemolyticus protein extracts, endotoxins, and related preparations. these results were probably due to the presence of large amounts of protein in the v. parahaem ...197993111
electron spin resonance studies on phaeomelanins.esr spectra of synthetic melanins formed from dopa and from 5-s-cysteinyldopa and of melanins isolated from human melanomas were studied. alkali-soluble melanin synthesized from cysteinyldopa showed a signal similar to that of insoluble melanin synthesized from dopa, but also a simple fine structure. trichochromes c and f synthesized from 5-s-cysteinyldopa had a broader signal than dopamelanin, and in addition a hyperfine structure that could be explained by the presence of manganous ions. an in ...197993370
relationship of seminal volume to size and disease of the prostate in the beagle.the seminal volume of young adult beagles was correlated with the size of and disease in their prostates. the ejaculate volume of naturally collected semen increased in a linear manner with prostatic weight in dogs with normal or glandular hyperplastic prostates. this correlation was not observed in the dogs with cystic hyperplasia, in which there was a reduction in volume of 50% in the semen produced for each gram of prostate. there were no significant differences in other seminal characteristi ...197993426
symposium on uropathology. 197993566
antigenic heterogeneity of the non-serogroup antigen structure of neisseria gonorrhoeae lipopolysaccharides.studies of the antigenic structure of the polysaccharide component of gonococcal lipopolysaccaride (lps) indicated that the non-serogroup antigen structure is antigenically heterogeneous. immunodiffusion studies of gc4 strain 8551 indicated that in addition to the gc serogroup determinent, this polysaccharide contains two other sets of determinants, one which is shared with the other five gc serogroups and a second which is shared by the gc1 and gc3 serogroups. enzyme-linked immunosorbent assay ...197993575
the binding of streptomycin to ribonucleotides.incubation of streptomycin (sm) with [32p]5'-ribonucleotides at ph 7.0 produces fractions that migrate towards the cathode in high voltage electrophoresis (hve) separations at ph 3.5. sm appears to interact with pg, pa and pc but not with pu. the appearance of these [32p]-labeled fractions is dependent on incubation time and sm concentration. incubation of nucleotides with dihydrostreptomycin (dsm) or sm reduced with sodium cyanoborohydride (nabh3cn) at ph 5.0, does not produce detectable change ...197993596
inhibition of calcium-dependent regulator-stimulated phosphodiesterase activity by neuroleptic drugs is unrelated to their clinical efficacy. 197993689
location of bladder preganglionic neurons within the sacral parasympathetic nucleus of the cat.sacral parasympathetic preganglionic neurons innervating the urinary bladder were labelled by applying horseradish peroxidase (hrp) to branches of the pelvic nerve at the neck of the bladder. bladder preganglionic cells were located primarily in the intermediolateral grey matter of sacral cord segments 1, 2 and 3 and extended from the ventral end of the central canal to the inferior margin of the dorsal horn. dendrites extended into the dorsolateral funiculus, toward the lateral edge of the dors ...197993725
[allergenic properties of the blood serum of drug allergy patients]. 197993831
semicarbazide-sensitive amine oxidase in rat aorta: a plasma-membrane enzyme [proceedings]. 197993954
comparison of the antibodies elicited by the individual structural polypeptides of foot-and mouth disease and polio viruses.antibody produced against preparations of vp1, one of the four structural polypeptides of foot-and-mouth disease virus, neutralized the virus and reacted with both full and empty particles in radioimmunoassays (ria). antiserum against vp2 reacted with artificial empty particles of the virus but not with full particles. in contrast, none of the individual polypeptides of poliovirus produced antisera which neutralized the virus nor reacted with it in ria. however, antisera produced with vp1 and vp ...197994352
[a study of substrate in antinuclear antibody technique (author's transl)]. 197994362
reversal of serotonin vasodilatation in the dog external carotid bed by sympathetic view of the conflicting reports of both constrictor and dilator effects of serotonin on the external carotid vascular bed of dogs, the influence of intraarterial infusions of the amine on blood flow through this territory was assessed by electromagnetic flowmeter techniques. in anesthetized intact dogs, serotonin produced dose-related increases in flow. these vasodilator responses were markedly diminished after ipsilateral vagotomy and were followed by delayed vasoconstriction, which then bec ...197994376
mechanism of antihypertensive activity of orally administered prazosin in spontaneously hypertensive conscious, spontaneously hypertensive rats (shr) oral prazosin (0.03-3.0 mg/kg) resulted in dose-related reductions of systolic blood pressure measured with a tail cuff. in shr whose tail artery blood pressure was continuously monitored the antihypertensive effect of prazosin (1.0 mg/kg, p.o.) was accompanied by a significant increase in heart rate. several groups of shr were pithed 2 hr after oral prazosin (1.0 mg/kg) or placebo. in this preparation the mean carotid blood pressure increases ...197994380
effect of silica on a culture of rat peritoneal macrophages. 197994527
a biochemical comparison of development of various facial bones in neonatal rats. 197994540
new surgical management in the cleft palate repair and its histological aspects. 197994731
effect of lysolecithin on pig platelet collagen : glucosyltransferase [proceedings]. 197994798
[the heterogeneity of streptokinases].purified streptokinases of some strains of different types of strep. pyrogenes (group a) as well as streptokinases of a strain of group c or group g streptococci, resp., were studied in regard of their heterogeneity. differences of the streptokinases were found in their amino acid compositions and in isoelectric points. serological differences were detected by a quantitative precipitation reaction with fluorescein-coupled antistreptokinase antisera from the goat as well as by neutralization reac ...197994885
heterogeneity, immunological comparison and concentration profiles of alpha-fetoproteins derived from late-gestational and early postnatal mouse tissue.four distinct alpha-fetoprotein (afp) species were separated by ion-exchange chromatography of the approximately 70 000 dalton fraction of an extract of late-gestation fetal mice. although chromatographicaly heterogeneous, antigenic differences among the afp species resolved were not detected using classical double-diffusion methods. analysis of the afp contribution to total body saline-extractable protein of fetal and neonatal mice revealed an abrupt decrease in the fetal afp contribution to to ...197995198
inhibition by isoproterenol of ovalbumin-induced contraction of tracheal strips and release of histamine from lung isolated from the actively sensitized guinea-pig. 197995248
computer-controlled double-beam scanning microspectrophotometry for rapid microscopic image reconstructions.a method for automated collection of various specific data from an entire microscopical preparation and their quantitative evaluation is described. its application to the study of neuronal connections is discussed in some detail. brain sections are scanned using a computer-controlled microscope for reflectance, fluorescences or absorbance signals. two illuminating beams are used, one of them being amplitude modulated. by means of a synchronous detection the two signals are recorded simultaneousl ...197995455
transformation of marmoset lymphocytes in vitro with herpesvirus ateles.circulating lymphocytes from three species of marmoset monkeys were transformed in vitro with herpesvirus ateles (hva) either by co-cultivation with lethally x-irradiated hva-producing lymphoblastoid cells or by infection with cell-free virus: 42 transformed lymphocyte cultures were obtained from 72 transformation attempts. attempts to transform squirrel monkey lymphocytes were unsuccessful in 29 attempts. association of hva with each transformed culture was demonstrated by staining of antigen-p ...197896033
lower levels of thyrotropin-releasing hormone-degrading activity in human cord and in maternal sera than in the serum of euthyroid, nonpregnant adults.thyrotropin-releasing hormone (trh)-degrading activity was investigated in human cord, maternal, and euthyroid adult sera by measuring (a) the rate of disappearance of trh and (b) the rate of formation of degradation products. the rate of trh degradation in cord and maternal sera was 25-33% of that in euthyroid adult serum. concomitantly, in cord and maternal sera, the rate of formation of proline, a major trh degradation product in serum, was one-quarter to one-third that in euthyroid adult ser ...197896140
pseudomonas cytochrome c551 at 2.0 a resolution: enlargement of the cytochrome c family.the structure of respiratory cytochrome c551 of pseudomonas aeruginosa, with 82 amino acids, has been solved by x-ray analysis and refined to a crystallographic r factor of 16.2%. it has the same basic folding pattern and hydrophobic heme environment as cytochromes c, c2, and c550, except for a large deletion at the bottom of the heme crevice. this same "cytochrome fold" appears to be present in photosynthetic cytochromes c of green and purple sulfur bacteria, and algal cytochromes f, suggesting ...197896440
comparison of bacterial cardiotoxins: thermostable direct hemolysin from vibrio parahaemolyticus, streptolysin o and hemolysin from listeria monocytogenes.some properties of the bacterial cardiotoxins, thermostable direct hemolysin from vibrio parahaemolyticus (vibriolysin), and streptolysin o and hemolysin from listeria monocytogenes (listeriolysin), were compared. these toxins had rapid lethal effects on mice when injected intravenously. the electrocardiographic changes of rats after intravenous injections of these toxins were very similar, showing bradycardia and inhibition of atrio-ventricular conduction. these toxins also caused cessation of ...197896814
[physico-chemical properties of microbial antigens and the immuno-allergic tests].experiments were conducted on guinea pigs sensitized by the delayed and immediate types of allergy. different antigens obtained from the strain of brucella abortus ba-19 were used for sensitization and the resolving action. comparison of the resolving properties of the corpuscular, soluble (ultrasound treated) antigens and purified protein fractions, polysaccharide and rna was carried out in the skin reactions of the immediate and delayed type, passive skin anaphylaxis, acute anaphylactic shock, ...197896888
[serological survey of animal rickettsioses in cameroon. i. evaluation of the threshold of positivity and specificity of the micro-agglutination reaction as related to the size of the animal]. 197796947
nitrate reductase activity of mycobacterium tuberculosis and mycobacterium bovis in the presence of electron donors.the activities of the nitrate reductase enzyme of mycobacterium tuberculosis, m. bovis, and of bcg were assayed with and without addition of electron donors. m. tuberculosis always reduced nitrate; m. bovis did so only in the presence of electron donors, and bcg did not show enzymatic activity.197897306
cerebral prostaglandin synthesis during the dietary and pathological stresses of essential fatty acid deficiency and experimental allergic encephalomyelitis.rats of the lewis strain were fed diets adequate or deficient in essential fatty acids (efa). at 70-80 days of age experimental allergic encephalomyelitis (eae) was induced using adjuvants containing either mycobacterium butyricum or mycobacterium tuberculois h37ra. when the former mycobacterium was used, the incidence of eae was greater in the efa-deficient than in efa-adequate controls; but when the rats challenged with m. tuberculosis, the incidence of the disease was the same in both dietary ...197897486
[passage of endogenous urea into a jejunum of sheep].the quantitative passage of urea-n and ammonia n into an isolated jejunum of the merino sheep was studied within one hour before, and 1, 2, 3, 4 and 5 hours after feeding. the total amount of ammonia-n that passes to the isolated jejunum [to both proximal and distal part] within an hour is small [0.28-0.35 mg h-1], without any significant differences in passage prior to and after feeding. however, urea-n secretion is high, especially secretion to the fore part of the jejunum [ 3.35-3.62 mg h-1 ...197897837
altered mrna metabolism in ribonuclease iii-deficient strains of escherichia coli.the metabolism of mrna from the lactose (lac) operon of escherichia coli has been studied in ribonuclease (rnase) iii-deficient strains (rnc-105). the induction lag for beta-galactosidase from the first gene was twice as long, and enzyme synthesis was reduced 10-fold in one such mutant compared with its isogenic rnc+ sister; in the original mutant strain ab301-105, synthesis of beta-galactosidase was not even detectable, although transduction analysis revealed the presence of a normal lac operon ...197898520
allogeneic restriction of acquired antimicrobial resistance in the rat.allogeneic restriction of acquired antimicrobial resistance was demonstrated by infusing listeria-immune thoracic duct lymphocytes (tdl) into syngeneic, semisyngeneic, or allogeneic recipients that had been infected i.v. with listeria monocytogenes (lm). syngeneic and semisyngeneic recipients enjoyed a high level of protection (3 to 5 log10 reduction in viable organisms within 64 hr, whereas allogeneic recipients were poorly protected (0.5 to 2.5 log10 reduction). sharing one haplotype between t ...197898584
changes in composition of tears of malnourished children. 197898738
[immunological relations between mother and child during pregnancy (author's transl)].the relations between the maternal and foetal organisms during pregnancy are partly very complicated and remain inexplicable. blastocyst, trophoblast, embryo and foetus have membrane antigens at a very early stage, and the immunocompetence of the foetus develops already during the second third of the pregnancy period. many non-specific factors are involved in preventing the castingoff reaction of the foetal organism. the trophoblastic tissue is probably of central importance. the focal point of ...197898927
improved safety of splenoportography by plugging of the needle tract.splenoportography, a useful preoperative diagnostic procedure in patients with portal hypertension, has been largely discontinued because of the risk of hemorrhage. we have used both ivalon and gelfoam in particle and plug form to plug the needle tract after splenoportography. initial studies were conducted on six dogs. plugging of the needle tract by injection of suspensions of small gelfoam or ivalon particles resulted in intravasation of these particles into the splenic vein. this was demonst ...197898987
fibrinogen turnover in pregnant rabbits during the first and last thirds of gestation.although thrombin-mediated fibrinogen derivatives have been observed in normal pregnancy, an increased turnover of fibrinogen has been suspected but has not yet been demonstrated. fibrinogen turnover was studied in 14 pregnant and 10 nonpregnant female rabbits by use of purified rabbit 125i-labeled fibrinogen. in the last third of gestation the half life of fibrinogen was shortened by 45% and the fractional catabolic rate increased by 44% in comparison to the values for the first third of gestat ...197899036
interferon inducing activity of polyinosinic acid.although poly(i) is generally considered to be inactive as an interferon inducer, we have found several authentic poly(i) preparations to be effective inducers. their interferon inducing ability varied considerably from one cell system to another. in human diploid fibroblasts, primed with interferon and superinduced by cycloheximide and actinomycin d, all active poly(i) samples proved nearly as effective in inducing interferon as poly(i).poly(c). in primary rabbit kidney cell cultures, the activ ...197899486
aflatoxin is degraded by mycelia from toxigenic and nontoxigenic strains of aspergilli grown on different substrates.the ability of 9-day-old mycelia of aspergillus parasiticus nrrl 2999 to degrade aflatoxin varied depending on the substrate used to grow the mold. substrates which allowed substantial mycelial growth yielded mycelia which actively degraded aflatoxin. substrates which allowed minimal growth of mycelia yielded mycelia with little ability to degrade aflatoxin. biodegradation of aflatoxin was also strain-dependent. a. parasiticus nrrl 2999 and nrrl 3000 actively degraded aflatoxin, a. flavus nrrl 3 ...197899656
evidence for a c-terminal tyrosine residue in human and mouse b-lymphocyte membrane mu chains. 197899661
[reservoirs and vectors of trypanosoma cruzi. lxix. preliminary survey of wild triatominae in the region of triangulo mineiro, mg, brazil]. 197899771
purification and characterization of allergens from parietaria officinalis pollen. 1978101439
mortality of monkeys after exposure to fission neutrons and the effect of autologous bone marrow order to assess the risk of exposure to ionizing radiation in man, and to evaluate the results of therapeutic measures, the mortality of rhesus monkeys irradiated with x-rays and fission neutrons and the effect of autologous bone marrow transplantation have been investigated. the ld50/30d values for x- and neutron-irradiated monkeys amount to 525 and 260 rad respectively, resulting in an r.b.e. of approximately 2 for the occurrence of the bone marrow syndrome. protection of the animals by aut ...1978101475
chromosome-membrane association in bacillus subtilis. iv. further purification of dna-membrane complex by using a combination of centrifugation and electrophoresis.we have developed a simple procedure to purify a dna-membrane complex from bacillus subtilis by using a combination of centrifugation and electrophoresis. several unique proteins were detected in the purified complex.1978101532
[studies on moraxella. ii. studies on the identification of moraxella by biochemical character (author's transl)]. 1977101604
high affinity uptake sites for taurine in the retina. 1978104877
transcription and translation in a pleiotropic streptomycin-resistant mutant of escherichia coli.the role of the ribosomal protein s12 (streptomycin protein) in ribosome function and in other metabolic processes in the cell has been investigated. a spontaneous streptomycin-resistant strain of escherichia coli (sm3) carrying a mutation in the rpsl gene is deficient in its ability to induce the synthesis of the enzyme bets-galactosidase. it was demonstrated that the reduced rate of enzyme synthesis results from deficiencies in both the transcription of the lactose operon and translation of th ...1979104958
separation and characterization of highly purified forms of liver microsomal cytochrome p-450 from rats treated with polychlorinated biphenyls, phenobarbital, and 3-methylcholanthrene. 1979105007
implications for adult roles from differential styles of mother-infant bonding: an ethological study.ethological observations of maternal and infant behaviors of nine vervet monkey pairs (cercopithecus aethiops sabaeus) showed the effects of differential styles of early maternal responsiveness on later infant competence. those infants receiving the least amount of maternal responsiveness and the most time-off the mother in the first 3 months of development were more socially competent at 6 months of age. the results are discussed within current ethological "attachment" theories. the detachment ...1979105083
case report: intestinal clearance of calcium and protein in waldenstrom's macroglobulinemia.a 63-year-old man with waldenstrom's macroglobulinemia had severe steatorrhea, marked protein-losing enteropathy, and excessive endogenous fecal calcium clearance. the malabsorption and protein loss resulted in weight loss and hypoalbuminemia. in contrast, the striking enteric calcium loss was completely compensated by an increase in calcium absorption resulting in a positive calcium balance.1978105640
polychlorinated biphenyl-induced morphologic changes in the gastric mucosa of the rhesus monkey.the early morphologic events in polychlorinated biphenyl-induced lesions of the gastric mucosa of immature male rhesus monkeys (macaca mulatta) were studied in serial biopsies taken during consumption of diets containing 3, 10, 30, and 100 mg. per kg. (p.p.m.) of aroclor 1242. the severity of the lesions correlated directly with both the duration and the level of exposure. the first changes were seen after ingestion of 30 and 100 mg. per kg. for 2 weeks; changes were not seen until 10 weeks at 3 ...1979106183
[comparative study of methods for the bacterioscopic diagnosis of tuberculosis and of methods for determining drug sensitivity]. 1979106383
effects of morphine withdrawal on food competition hierarchies and fighting behavior in rats. 1979106426
carboxypeptidase of streptomyces griseus. implications of its characteristics. 1979106886
penicillin-resistant gonorrhea. 1979106997
overall pathway of mononucleosome production.five electrophoretically distinguishable classes of mononucleosomes (mi, mii, are produced upon treatment of mammalian nuclear chromatin with micrococcal nuclease. these five forms differ in their initial dna lengths, relative mass proportions, stability, contents of histone h1, and presence of certain nonhistone proteins. a new "chromatin fingerprinting" technique has been developed in order to trace nuclease-mediated interconversions between these mononucleosomes and their polynucleosom ...1979107168
electron capture gas-liquid chromatographic-mass spectral identification of acids produced by neisseria meningitidis in a defined medium.the acid metabolites and the cellular fatty acids of three strains of neisseria meningitidis grown in a chemically defined liquid medium were determined with computerized frequency-pulsed electron capture gas-liquid chromatography. five acids not previously reported were subsequently identified: isobutyric, octanoic, decenoic (c10:1), dodecenoic (c12:1), and tetradecenoic (c14:1). these acids were produced during active metabolism and were not detected as cellular constituents. the frequency-pul ...1979107187
halophilic, lactose-positive vibrio in a case of fatal septicemia.a halophilic vibrio species was isolated from blood cultures from a 59-year-old male with enteritis. the strain differed from vibrio parahaemolyticus and vibrio alginolyticus by its ability to ferment lactose, its production of beta-galactosidase, and its lower nacl tolerance. a report of this infection and a description of the isolate is presented.1979107189
radical scavenging as the mechanism for stimulation of prostaglandin cyclooxygenase and depression of inflammation by lipoic acid and sodium iodide.certain radical-trapping reducing agents have been shown to stimulate prostaglandin biosynthesis in vitro (1--6) and to depress phorbol myristate acetate-induced mouse ear edema (16). the increased prostaglandin synthesis resulted from influences on the cyclooxygenase. to ascertain whether these alterations were due to direct interaction with the enzyme or to indirect scavenging of the oxidant released during pgg2 reduction, we report the effects of lipoic acid and sodium iodide. both of these a ...1978107544
enteric infections in singapore with special reference to typhoid.a review of the epidemiological features of the more important enteric infections in singapore was given. enteric fevers (typhoid and paratyphoid), salmonella gastroenteritis and shigellosis remained endemic at a rather constant level in spite of vast improvement in environmental sanitation, but amoebic dysentery showed marked decline. vibrio parahaemolyticus is an important cause of food poisoning since it was first reported in 1973. el tor cholera is believed to be introduced through regional ...1978107597
a three month inhalation toxicity study in the squirrel monkey (saimiri sciureus) with terbutaline sulfate (bricanyl).a study of the subacute toxicity of inhaled terbutaline sulfate was performed in the squirrel monkey (saimiri sciureus). 3 groups of monkeys were exposed 1 h daily 7 days/week for 3 months to terbutaline sulfate aerosols at concentrations of 0.039, 0.185 and 0.799 mg terbutaline/litre of air respectively. a fourth group was a chamber control receiving air only. the following clinical parameters were evaluated: physical appearance and behavior, weight gain, ophthalmoscopic appearance, electrocard ...1978107616
pituitary-thyroid function in spironolactone treated hypertensive women.four weeks high dose spironolactone treatment (aldactone searle, 100 mg q. i. d.) significantly enhanced the tsh (delta max. 8.5 +/- 4.1 vs. 4.6 +/- 3.1 microunits/ml, p less than 0.05) and t3 (delta max. 32 +/- 27 vs. 11 +/- 16 ng/100 ml, p less than 0.05) responses to an intravenous trh/lh-rh bolus injection in 6 eumenorrhoeic euthyroid hypertensive women, without affecting basal serum tsh, t3 or t4 levels or the basal and stimulated lh, fsh and prolactin values (p greater than 0.10). the mean ...1979107696
experimental transmission of wuchereria bancrofti to monkeys.infective larvae of wuchereria bancrofti from laboratory-raised culex pipiens fatigans and aedes togoi mosquitoes fed on human volunteers in jakarta, indonesia (j strain) and kinmen island, china (k strain) were introduced into taiwan monkeys (macaca cyclopis) by subcutaneous inoculation, by foot puncture, or by permitting infected mosquitoes to feed weekly on the monkeys. some animals were splenectomized and others were treated with varying regimens of immunosuppressants. necropsy was done on m ...1979107818
isolation of listeria monocytogenes from animals in the sudan. 1979107996
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