| enzyme-catalyzed redox reactions with the flavin analogues 5-deazariboflavin, 5-deazariboflavin 5'-phosphte, and 5-deazariboflavin 5'-diphosphate, 5' leads to 5'-adenosine ester. | the ability of 5-deazaisoalloxazines to substitute for the isoalloxazine (flavin) coenzyme has been examined with several flavoenzymes. without exception, the deazaflavin is recognized at the active site and undergoes a redox change in the presence of the specific enzyme substrate. thus, deazariboflavin is reduced catalytically by nadh in the presence of the beneckea harveyi nad(p)h:(flavin) oxidoreductase, the reaction proceeding to an equilibrium with an equilibrium constant near unity. this i ... | 1976 | 3207 |
| purification and properties of the methane mono-oxygenase enzyme system from methylosinus trichosporium ob3b. | 1. a three-component enzyme system that catalyses the oxidation of methane to methanol has been highly purified from methylosinus trichosporium. 2. the components are (i) a soluble co-binding cytochrome c, (ii) a copper-containing protein and (iii) a small protein; the mol. wts. are 13 000, 47 000 and 9400 respectively. the cytochrome component cannot be replaced by similar cytochrome purified from pseudomonas extorquens or by horse heart cytochrome c. 3. the stoicheiometry suggests a mono-oxyge ... | 1977 | 15544 |
| cation binding by tobacco rattle virus. | | 1977 | 16380 |
| the tricarboxylic and acid pathway in desulfovibrio. | strains of two species of desulfovibrio were examined for enzymes of the tricarboxylic acid cycle and related pathways. pyruvate carboxylase (ec6.4.1.1) is present, and alpha-ketoglutarate is formed via the tricarboxylic acids. glutamate, but not succinyl-coa, arises from alpha-ketoglutarate. a pathway exists from pyruvate by malic enzyme (ec 1.1.1.39) activity to malate, then fumarate and succinate, again with no evidence of succinyl-coa formation. the enzymes concerned with metabolism of these ... | 1977 | 18274 |
| toxic interactions between disulfiram, and some centrally acting drugs in rats. | | 1978 | 22733 |
| studies of the control of luminescence in beneckea harveyi: properties of the nadh and nadph:fmn oxidoreductases. | highly purified nadh and nadph:fmn oxidoreductases from beneckea harveyi have been characterized with regard to kinetic parameters, association with luciferase, activity with artificial electron acceptors, and the effects of inhibitors. the nadh:fmn oxidoreductase exhibits single displacement kinetics while the nadph:fmn oxidoreductase exhibits double displacement or ping-pong kinetics. this is consistent with the formation of a reduced enzyme as an intermediate in the reaction of catalyzed by t ... | 1978 | 23827 |
| myristic acid stimulation of bacterial bioluminescence in "aldehyde" mutants. | the involvement of long chain aldehyde in bacterial luminescence was known both from its being required for light emission in the in vitro reaction with pure luciferase and from its ability to stimulate luminescence in vivo in a certain class of dark "aldehyde" mutants. we have found that the luminescence of some (but not all) of such aldehyde mutants is also stimulated by long chain aliphatic fatty acids, with a marked specificity for myristic (tetradecanoic) acid. this stimulation has been dem ... | 1978 | 24214 |
| continuous monitoring of reactions that produce nadh and nadph using immobilized luciferase and oxidoreductases from beneckea harveyi. | | 1978 | 29512 |
| the respiratory chain and proton electrochemical gradient in the alkalophile bacillus pasteurii [proceedings]. | | 1978 | 33860 |
| properties and uses of immobilized light-emitting enzyme systems from beneckea harveyi. | bacterial luciferase and nadh:fmn oxidoreductase have been immobilized onto arylamine glass beads. these immobilized enzymes can detect as little as 0.2 pmol of nadh per assay sample. glucose-6-phosphate dehydrogenase has been co-immobilized with these enzymes, and with this system it is possible to quantitate 1 pmol of glucose 6-phosphate. by co-immobilizing a fourth enzyme, hexokinase, onto the glass beads, the system can reproducibly detect 20 pmol of glucose per liter. these immobilized enzy ... | 1979 | 38021 |
| flavin mononucleotide reductase of luminous bacteria. | nad(p)h: fmn oxidoreductase (flavin reductase) couples in vitro to bacterial luciferase. this reductase, which is also postulated to supply reduced flavin mononucleotide in vivo as a substrate for the bioluminescent reaction, has been partially purified and characterized from two species of luminous bacterial. from photobacterium fischeri the enzyme has a m. w. determined by sephadex gel filtration, of 43,000 and may have a subunit structure. the turnover number at 20 degrees c, based on a purit ... | 1975 | 47604 |
| [development of c cells of the thyroid gland of the rat under an antithyroidian treatment inducing gamma tumors]. | in the normal ageing rat is observed a progressive hyperplasia of the c cells. in the rat treated by an antithyroideal drug, the hyperplasia is much more important than in normal. it already appears during the treatment and grows up after its stop. this observation means that the c cells hyperplasia, which reaches later to gamma tumors production, is not due to the tsh oversecreted under the antithyroideal drug influence. | 1975 | 58702 |
| purification of protein kinase of mouse sarcoma virus and its effect on reverse transcriptase activity. | | 1979 | 89847 |
| studies on the mechanism and specificity of inhibition of ribonucleic acid polymerase by linear gramicidin. | | 1979 | 91383 |
| occurrence of beta 2-microglobulin in mammalian lymphocytes and erythrocytes. | cell-associated beta 2-microglobulin (beta 2m) has been studied by quantitative techniques in lymphocytes and erythrocytes of guinea pig, rabbit, rat and man. beta 2m was solubilized by sonication followed by treatment with acid, deoxycholate or thiocyanate, and then determined by radioimmunoassay. the average number of beta 2m molecules per lymphocyte, estimated after acidification, varied between 0.89 x 10(5) and 7.1 x 10(5). rat erythrocytes contained 3.0 x 10(3) molecules per cell, whereas n ... | 1979 | 91519 |
| bacteriology of raw breast milk. | | 1979 | 91911 |
| response to immunological or cholinergic stimulation of isolated mast cells from man, guinea pig and rat. | | 1979 | 91954 |
| [cutaneous leishmaniasis. alcoy: an endemic zone]. | | 1979 | 92178 |
| immunological cross-reactivity between acid extracts of myelin, liver and neoplastic tissues: studies in immunized guinea-pigs. | groups of 4 guinea-pigs were immunized with acid extracts prepared from bovine myelin (ef), normal human liver tissue and malignant or benign neoplastic tissues in freund's complete adjuvant (fca1. the animals were weighed daily and examined for clinical signs of experimental allergic encephalomyelitis (eae). all the animals immunized with ef developed clinical symptoms of eae within 21 days of the initial immunization, whilst some of the animals immunized with certain tumour extracts developed ... | 1979 | 92328 |
| guinea-pig alpha 1 microglobulin. isolation and properties in comparison with human alpha 1 microglobulin. | | 1979 | 92407 |
| electron spin resonance studies on phaeomelanins. | esr spectra of synthetic melanins formed from dopa and from 5-s-cysteinyldopa and of melanins isolated from human melanomas were studied. alkali-soluble melanin synthesized from cysteinyldopa showed a signal similar to that of insoluble melanin synthesized from dopa, but also a simple fine structure. trichochromes c and f synthesized from 5-s-cysteinyldopa had a broader signal than dopamelanin, and in addition a hyperfine structure that could be explained by the presence of manganous ions. an in ... | 1979 | 93370 |
| symposium on uropathology. | | 1979 | 93566 |
| chlorpyrifos and 3,5,6-trichloro-2-pyridinol: residues in body tissues of swine treated with chlorpyrifos for hog louse and itch mite control. | | 1979 | 93608 |
| a simple method for staining semi-thick tissue sections to demonstrate fungal elements. | a simple method of staining epon embedded semi-thick sections with schiff-reagent is described. this technique allows fungal elements in tissue to be readily observed. the combination of schiff-reagent with a methylene blue counterstain for demonstrating fungal elements and tissue structures is also described. | 1979 | 93702 |
| human pl cells grown as continued t-cell cultures. | | 1979 | 93820 |
| study of effectiveness of bleomycin in rat brain tumor model intravenously and intracerebrally. | | 1979 | 94056 |
| disopyramide plasma and myocardial tissue concentrations as they relate to antiarrhythmic activity. | antiarrhythmic concentrations of disopyramide in canine plasma and myocardium were determined by gas chromatography. ventricular tachycardia was incuded in anesthetized dogs by the intravenous administration of ouabain. disopyramide phosphate was then administered by a two-stage continuous infusion method. a rapid infusion of disopyramide (9.08 mg/kg/hr) was administered for 30 min, followed by a slow infusion (2.18 mg/kg/hr) to maintain steady-state plasma levels of 1.98-2.21, mean +/- sem = 2. ... | 1979 | 94410 |
| structure-activity relationships of an aminosugar cardiac glycoside, asi-222 hcl, in the heart-lung preparation of the dog. | asi-222 [3-beta-o-(4-amino-4,6-dideoxy-beta-d-galactopyranosyl) digitoxigenin hcl] is a semisynthetic aminosugar cardiac glycoside that has been shown to have a greater therapeutic index than ouabain or digoxin in dogs. we have compared the effects of asi, digitoxigenin, digitoxigenin-beta-d-galactose, and digoxin in the dog heart-lung preparation. minute work and stroke work were calculated. controls were obtained before and after the hearts were failed with sodium pentobarbital. asi-222 is abo ... | 1979 | 94411 |
| influence of substance p on mesenteric hemodynamics in the pig. | | 1979 | 94532 |
| a biochemical comparison of development of various facial bones in neonatal rats. | | 1979 | 94540 |
| sharing ia antigens between species. iii. ia specificities shared between mice and human beings. | certain mouse alloantisera have been found to detect immunologic cross-reactions between human and murine ia antigens. almost every anti-iaa, -iak and -iad serum tested exhibited such cross-reactions. sera prepared against the products of limited segments of the mouse i region and tested on human b cells revealed that anti-i-e/ck cross-reactions were more readily detectable than anti-i-a, b, jk cross-reactions. most of the mouse alloantisera were cytotoxic to bells from almost every individual t ... | 1979 | 94693 |
| de novo purine synthesis in cultured human fibroblasts. | | 1979 | 94758 |
| induction of monoclonal antibody synthesis in malignant human b cells by polyclonal b cell activators. relationship between b cell subsets and prognosis. | | 1979 | 94897 |
| a silver carbonate method for cell counts of neurons and glial elements on paraffin embedded brain tissue. | a modification of the del rio-hortega method for the demonstration of central nervous system elements is presented. this silver impregnation technique is particularly useful for the classification of cell types for quantitative differential cell counts. formalin fixed paraffin sections are immersed in formol-ammonium bromide for 1 1/2 hours; this solution is an excellent mordant for various silver nitrate stains. the samples are stained for 20 to 60 minutes in a silver carbonate solution (25 ml ... | 1979 | 94949 |
| treatment of severe herpes keratitis with cryosurgery and immunotherapy. | the contrasting type of lesion produced in the first eye of non-immune and the second eye of the then immune rabbits using herpes simplex type i virus is demonstrated. cryosurgery may make the first eye worse but the second eye is healed. a large percentage of second eyes heal spontaneously, however, as circulating antibody titres are high. artefacts may be mistaken for lesions in the immune eye but superficial viral growth has a characteristic plaque-like appearance. one hundred eyes with recur ... | 1979 | 95060 |
| biochemical and immunochemical studies of alpha-amylase from the salivary glands of the mouse. | | 1979 | 95093 |
| vitamin a effect on tumor angiogenesis. | the inhibitory effect of vitamin a on tumor establishment and growth has been studied in two animal models. the c57l/j hepatoma, when placed in c57l/j mice receiving inoculations of vitamin a, showed slow growth and the hosts had significantly prolonged survival over untreated mice. the v-2 carcinoma, when implanted in the corneas of new zealand white rabbits receiving injections of vitamin a, showed decreased vascular response in the limbic vessels. the absence of an induced vascular response p ... | 1979 | 95197 |
| efficacy of penicillin g, cefazolin, and gentamicin in m-k medium at 4 degrees c. | corneal-scleral shells are routinely stored in mccarey-kaufman (m-k) medium at 4 degrees c. antibiotics are added to the medium to suppress bacterial growth. we examined the antibacterial activity of penicillin g potassium, 100 microgram/ml, cefazolin sodium, 100 microgram/ml, and gentamicin sulfate, 20 microgram/ml against test organisms in m-k medium at 4 degrees, 23 degrees, and 37 degrees c for periods up to 18 hours. within the limits of the experimental conditions, none of the antibiotics ... | 1978 | 96782 |
| meningitis caused by neisseria meningitidis type w-135. | | 1978 | 97374 |
| biochemical changes in guinea pig lungs due to amosite asbestos. | | 1978 | 98326 |
| use of t4 rna ligase to construct model substrates for a ribosomal rna maturation endonuclease. | rnase m5 of bacillus subtilis specifically cleaves a 179-nucleotide precursor 5s rrna to yield mature 5s rrna (116 nucleotides) and two fragments derived from the termini. possible recognition elements for rnase m5 within the precursor structure include nucleotide sequences arranged with 2-fold rotational and translational symmetry about the substrate bonds. we have used bacteriophage t4 rna ligase to construct, from synthetic oligonucleotides and mature or precursor 5s rrna fragments, test subs ... | 1978 | 98766 |
| effects of chronic lead exposure on levels of acetylcholine and choline and on acetylcholine turnover rate in rat brain areas in vivo. | rats were exposed to lead acetate from birth, and were killed at the age of 44--51 days for analysis of levels and turnover rates of acetylcholine (ach). steady-state levels of ach were not altered in midbrain, cortex, hippocampus, or striatum of lead-exposed rats. similarly, no changes in choline (ch) concentrations were found in cortex, hippocampus, or striatum. in the midbrain, however, a 30% reduction in ch levels was observed. changes in specific activity of ch and ach were measured as a fu ... | 1978 | 98796 |
| experimental spinal cord trauma, ii: blood flow, tissue oxygen, evoked potentials in both paretic and plegic monkeys. | graded clinical motor deficits were produced in a series of rhesus monkeys subjected to experimental spinal cord trauma from a variety of impact loads. an argon washout technique was used to measure spinal cord blood flow; tissue oxygen carbon dioxide, and somatosensory evoked potentials (seps) were also monitored. each animal received a clinical grade and, after a week of study, the spinal cords were removed for histopathological grading. blood flow in paraplegic animals was significantly decre ... | 1978 | 98856 |
| age-associated changes in the blood picture of the goat. | | 1978 | 98936 |
| biological variation of immunoglobulin concentrations in normal human tears related to age and sex. | single radial immunodiffusion method was used to measure the concentration of igg, iga, igm and igd in tears of 220 healthy individuals aged from 2 to 86 years. relation of the values to age and sex has been evaluated statistically by regression analysis method. mean iga level was 30.7 mg/100 ml. igg could be detected in 200 samples and the level was less than 1 mg/100 ml. igm was detected in only 7 samples and the value was less than 1 mg/100 ml. igd could not be detected in any of the sample. ... | 1978 | 98956 |
| fluorometric assay for measuring biological half-life of coralyne sulfoacetate in dogs and monkeys. | a spectrophotofluorometric assay for coralyne sulfoacetate was developed. caralyne was extracted from serum samples with n-butyl alcohol, and the drug concentrations were determined fluorometrically at 475 nm when the extract was excited at 325 nm. a biphasic serum decay curve for coralyne was observed for both dogs and monkeys. the biological half-lives for the two phases were 20 and 196 min in dogs and 15 and 142 min in monkeys. | 1978 | 99510 |
| [picosecond energy transfer between the spectral forms of pigments from the reaction center of rhodospirillum rubrum]. | absorption changes of reaction centers from rhodospirillum rubrum at 748, 796 and 870 nm induced by 532 and 870 nm picosecond light pulses were investigated with a picosecond spectrometer. kinetics of absorption changes at 748 and 796 had an additional bleaching when induced by the 532 nm pulse, in comparison with those at 870 nm. the additional bleaching was interpreted as a result of the excitation energy transfer via spectral forms of pigments of reaction centers. the experimental results fit ... | 1978 | 100143 |
| the diet and feeding behaviour of cercopithecus aethiops tantalus. | the feeding behaviour of representative groups of tantalus monkeys (cercopithecus aethiops tantalus) was observed during 1974-1975 in three habitats in cameroon. observations took place during the wet and dry seasons in sahelian savannah, guinea savannah and farmed forest. the tantalus monkeys were similar to other races of c.aethiops that have been studied in that they were omnivorous and lacked any narrow specialisations of either diet or feeding techniques. the dietary range was similar in al ... | 1978 | 101428 |
| murine macrophage-lymphocyte interactions: scanning electron microscopic study. | light and scanning electron microscopic observations revealed murine macrophage-lymphocyte interactions involving the initial contact of peritoneal, spleen, or thymus lymphocytes with peritoneal macrophage processes or microprocesses followed by clustering of lymphocytes over the central nuclear area of the macrophages. lymphocyte-lymphocyte clustering was not observed in the absence of macrophages. attachment and subsequent clustering appeared not to require the presence of serum or antigen; th ... | 1978 | 101458 |
| dominant constitutive mutations in malt, the positive regulator gene of the maltose regulon in escherichia coli. | | 1978 | 101676 |
| the effect of dinitrochlorobenzene (dncb) on dimethylbenzathracene (dmba) carcinogenesis on hamster buccal pouch. | dncb is an antigen that stimulates the cell-mediated response in a sensitized host. the purpose of this study was to define the effect dncb would have on a standard model system for oral carcinogenesis, theorizing that tumorgenesis would be delayed or inhibited. fifty-six syrian hamsters were divided into four groups. in group a (dncb/dmba), the right buccal pouch of twenty animals was treated with 2% dncb in orabase, twice a week, and 0.5% dmba, three times a week, for 10 weeks. in group b, twe ... | 1978 | 101932 |
| short-term memory in the rhesus monkey: effects of dopamine blockade via acute haloperidol administration. | the effects of dopaminergic blockade on recent or short-term memory (stm) were evaluated in test-sophisticated rhesus monkeys. each monkey was tested under several doses of the antidopaminergic haloperidol (0.006 to 0.05 mg/kg), in an automated, delayed-response procedure. the same procedure and test apparatus had previously been used to demonstrate profound stm impairments in aged rhesus monkeys and strikingly similar deficits in young monkeys given the anticholinergic scopolamine. the results ... | 1978 | 101990 |
| mechanisms and significance of qrs macrovoltage in the absence of cardiac hypertrophy. | the electric stimulation of the lateral ventricular walls carried out in experiments on dog heart (open-thorax), as well as the analysis of the clinical, radiological and electrocardiographic data recorded in 462 cases with qrs macrovoltage led to the following conclusions: a) in 22% of the cases (hospital cardiologic examinations) this anomaly cannot be accounted for either by age, blood pressure or cardiac hypertrophy; b) a temporarily perturbed development of the ventricular depolarization, i ... | 1978 | 101995 |
| homovanillic acid in human csf: comparison of fluorimetry and gas chromatography-mass spectrometry. | | 1978 | 103678 |
| implications for adult roles from differential styles of mother-infant bonding: an ethological study. | ethological observations of maternal and infant behaviors of nine vervet monkey pairs (cercopithecus aethiops sabaeus) showed the effects of differential styles of early maternal responsiveness on later infant competence. those infants receiving the least amount of maternal responsiveness and the most time-off the mother in the first 3 months of development were more socially competent at 6 months of age. the results are discussed within current ethological "attachment" theories. the detachment ... | 1979 | 105083 |
| mutagenicity of 4-nitropyridine-1-oxide for salmonella typhimurium. | | 1978 | 106271 |
| effects of morphine withdrawal on food competition hierarchies and fighting behavior in rats. | | 1979 | 106426 |
| congenital partial hemimelia tibia in a kitten. | | 1979 | 106597 |
| cavitary pulmonary lesions complicating use of flow-directed balloon-tipped catheters in two cases. | | 1979 | 106701 |
| the cellulolytic enzymes of botryodiplodia theobromae pat. separation and characterization of cellulases and beta-glucosidases. | 1. filtrates from cultures of different ages of botryodiplodia theobromae pat. were fractionated by gel filtration, ion-exchange chromatography and polyacrylamide-gel electrophoresis. 2. five cellulases (c1, c2, c3, c4 and c5) were found, and their molecular weights, estimated by gel filtration, were 46000-48000 (c1), 30000-35000 (c2), 15000-18000 (c3), 10000-11000 (c4) and 4800-5500 (c5). 3. cellulase c5 was absent from old culture filtrates. 4. cellulase c1 had little or no activity on cm-cell ... | 1979 | 106849 |
| metabolism of diethylstilbestrol: identification of a catechol derived from dienestrol. | the enzymatic oxidation of e-3,4-bis-(p-hydroxyphenyl)-hex-3-ene (diethylstilbestrol) by either mushroom tyrosinase or rat liver microsomes in the presence of nadph and air yields a catechol. upon further oxidation of both compounds with periodate and condensation of the resulting o-quinones with o-phenylenediamine, phenazines are produced. the phenazines derived from the products of both the plant and animal enzyme systems are identical to the product obtained by oxidation of diethylstilbestrol ... | 1978 | 106901 |
| tumor-host responses to various nutritional feeding procedures in rats. | parenteral and enteral nutrition are being used as adjuncts to cancer therapy. a liquid diet formulation containing a 27% solution of glucose and 3.9% crystalline amino acids with electrolytes and vitamins was given continuously for a week via parenteral (iv), and via intragastric (ig) routes and also was given ad libitum via the oral or per os (po) route to groups of buffalo rats with and without a morris no. 7777 transplantable hepatoma to find out how these feeding procedures affect tumor-hos ... | 1979 | 107299 |
| rna polymerase binding sites and transcription map of the dna of bacillus subtilis phage phi29. | | 1979 | 107317 |
| [semiessential and nonessential amino acids in parenteral feeding]. | with reference to a critical study of the relevant literature and to results from their own investigations, the authors emphasize the importance of the semi-essential and non-essential amino acids arginine, histidine, tyrosine, cystine and glutamic acid for the completion of essential amino-acid mixtures destined for parenteral feeding. madden's assumption that intravenously applied glutamic acid is intolerable was not supported by the authors' experiments in dogs. as evidenced by the increase o ... | 1978 | 107453 |
| a three month inhalation toxicity study in the squirrel monkey (saimiri sciureus) with terbutaline sulfate (bricanyl). | a study of the subacute toxicity of inhaled terbutaline sulfate was performed in the squirrel monkey (saimiri sciureus). 3 groups of monkeys were exposed 1 h daily 7 days/week for 3 months to terbutaline sulfate aerosols at concentrations of 0.039, 0.185 and 0.799 mg terbutaline/litre of air respectively. a fourth group was a chamber control receiving air only. the following clinical parameters were evaluated: physical appearance and behavior, weight gain, ophthalmoscopic appearance, electrocard ... | 1978 | 107616 |
| pituitary-thyroid function in spironolactone treated hypertensive women. | four weeks high dose spironolactone treatment (aldactone searle, 100 mg q. i. d.) significantly enhanced the tsh (delta max. 8.5 +/- 4.1 vs. 4.6 +/- 3.1 microunits/ml, p less than 0.05) and t3 (delta max. 32 +/- 27 vs. 11 +/- 16 ng/100 ml, p less than 0.05) responses to an intravenous trh/lh-rh bolus injection in 6 eumenorrhoeic euthyroid hypertensive women, without affecting basal serum tsh, t3 or t4 levels or the basal and stimulated lh, fsh and prolactin values (p greater than 0.10). the mean ... | 1979 | 107696 |
| experimental transmission of wuchereria bancrofti to monkeys. | infective larvae of wuchereria bancrofti from laboratory-raised culex pipiens fatigans and aedes togoi mosquitoes fed on human volunteers in jakarta, indonesia (j strain) and kinmen island, china (k strain) were introduced into taiwan monkeys (macaca cyclopis) by subcutaneous inoculation, by foot puncture, or by permitting infected mosquitoes to feed weekly on the monkeys. some animals were splenectomized and others were treated with varying regimens of immunosuppressants. necropsy was done on m ... | 1979 | 107818 |
| role of physiologic autoantibody in the removal of senescent human red cells. | the mechanism by which mononuclear phagocytes distinguish mature "self" from senescent "self" was investigated. evidence is presented indicating that human mononuclear phagocytes distinguish senescent rbc from mature rbc on the basis of selective ig attachment to the membranes of senescent cells. this ig, eluted from senescent human rbc, was shown to be igg and free of other igs by immunodiffusion, immunoelectrophoresis, and polyacrylamide gel electrophoresis. the igg was polyclonal with respect ... | 1978 | 108480 |
| glomerular basement membrane thickness following islet transplantation in the diabetic rat. | glomerular basement membrane (gbm) thickness was measured in diabetic rats prior to and following islet transplantation. over the course of the experiment (from 7 to 13 months of diabetes) gbm thickness in diabetic animals exceeded that of littermate controls. after 7 months of diabetes a group of animals received successful intraportal transplants of neonatal pancreatic tissue. gbm thickness at 2 and 6 months following islet transplantation matched the thickness in nontransplanted diabetic rats ... | 1979 | 110982 |
| the characterization of mason-pfizer monkey virus-induced cell fusion. | | 1979 | 111409 |
| interaction of the mannosephilic lectins of pseudomonas aeruginosa with luminous species of marine enterobacteria. | the marine bacteria beneckea harveyi and photobacterium leiognathi were shown to bear mannose-containing binding sites for the mannosephilic lectins of pseudomonas aeruginosa and concanavalin a (con a). the interaction between the lectins and the marine bacteria was demonstrated by the bacteriagglutination test, by adsorption of the lectins onto the bacteria and by mannose-specific peroxidase-binding to the lectin-coated bacteria. treatment of the bacteria with formaldehyde, phenol, ethanol or b ... | 1979 | 120927 |
| structural studies of heparitin sulfates. | heparitin sulfate fractions with a large range in sulfate content were subjected to degradation by flavobacterium heparinase and by nitrous acid. the products obtained were fractionated by chromatography, characterized, and used to arrive at tentative structures for these complex polysaccharides. the heparitin sulfate chains examined appear to be composed of: 1. uninterrupted blocks of n-acetylglucosamine containing disaccharides; 2. larger blocks with a molecular weight range of 5000 to 6000 wh ... | 1975 | 123778 |
| fatal immune complex glomerulonephritis without deposits. | repeated intravenous injections of egg albumin in rabbits produced small antigen-excess complexes and severe glomerulonephritis. immunoglobulins and complement in the glomeruli were not clearly demonstrated by immunofluorescence; deposits were found to be sparse by electron microscopy. this study demonstrates that soluble immune complexes are responsible for the glomerular reaction. the apparent absence of deposits is thus not sufficient to exclude an immune complex pathogenesis. | 1975 | 123971 |
| [correlation between uterine weight and endometrial development in rabbits treated with progesterone]. | progesterone induced effect on rabbit uterus follows a log dosis endometrial glandular changes linear relationship and log dosis-organ weight one. it appears a strong correlation between endometrial changes and organ weight. thus organ weight may be looked like a pseudogestative activity test, histological investigation remaining the actual proof of specific activity. besides these relations bring direct informations on the quality of new progestatives. | 1975 | 126770 |
| the significance of coronary arterial stenosis during cardiopulmonary bypass. | myocardial infarction may develop during an uneventful open-heart operation. in order to better understand this complication, we undertook an experimental study. the left circumflex coronary artery of 20 dogs was narrowed to 50 per cent of its area by a metal screw clamp to produce a localized coronary stenosis. regional myocardial perfusion in the distribution of both the stenotic circumflex and normal left anterior descending (lad) coronary arteries was measured by injection of a radioactive-l ... | 1975 | 127092 |
| [influence of long-term swimming training on the structure and enzyme activity of myosin in the rat myocardium (author's transl)]. | intermittent hemodynamic loading of the rat myocardium due to swimming training for several weeks leads to a significant increase in the specific atpase activity of myosin. this enzymatic alteration of the myosin molecule is accompanied by changes in the stoichiometry of its light chains which are of great significance for the atpase activity. the maximum shortening velocity of the unloaded myocardium (vmax), estimated on the basis of afterloaded contractions, shows a slight increase as a result ... | 1975 | 127740 |
| [cyto-immunological study of the anterior pituitary somatotropin cells of male and female adult peking, barbary and hybrid (from crossing male peking x female barbary) ducks and of male ducklings of the 3 races)]. | the anti-human-sth immunserum applied on hypophysis sections of adults males and females peking (anas platyrhynchos l.) barbary (cairina moschata l.) and hybrid (from the mating male peking x female barbary) ducks, marks orangeophils, oval- or round shaped, pas negative cells exclusively localized in the caudal lobe of the pars distalis. in the males ducklings of the three races this immunserum reveals numerous and partially exhausted cells. the authors conclude that the acidophils alpha cells o ... | 1976 | 134787 |
| [variations of various enzymatic activities of the krebs cycle (malate dehydrogenase, isocitrate dehydrogenase, succinate dehydrogenase) during experimental ischemic shock in the rat. influence of adenosine 5' triphosphoric acid]. | during the ischemic shock caused by the removal of tourniquets placed on the hind paws of the rat, a marked decrease in the enzyme activities of krebs cycle yielding atp (malate dehydrogenase, isocitrate dehydrogenase, succinate dehydrogenase) at the level of the gastrocnemius muscle and the liver, was observed together with a plasma increase of these enzymes. the intraperitoneal injection of atp diminishes significantly the variations observed. | 1976 | 134805 |
| [synchronous electric activity with inspiration in the vastus lateralis muscle of the anesthesied dog breathing spontaneously]. | in the dog, under chloralose or pentobarbitone anesthesia and breathing spontaneously, a muscular electrical activity of low voltage synchronous with respiration was observed in m. vastus lateralis. the mean maximum amplitude of this activity is inversely linked with respiratory rate. when the respiratory frequency is equal or exceeds 25 cycles per minute this activity disappears. such a phenomenon seems to be related to the activity of the respiratory centre. | 1976 | 134813 |
| mechanism of action of granulopoiesis inhibiting factor (chalone). i. evidence for a receptor protein on bone marrow cells. | rat bone marrow cells respond to granulopoiesis inhibiting factor by a reduced incorporation of tritiated thymidine. cells treated with low concentrations of trypsin lose their ability to respond to the factor if protein synthesis is partially inhibited by low doses of cycloheximide. responsiveness is retained if protein synthesis is permitted after enzyme treatment. the data suggest that a protein receptor on the external surface of the target cells is required for the action of granulopoiesis ... | 1975 | 134902 |
| [isolation of vad-medani arbovirus from hyalomma asiaticum sch. et schl., 1929 in turkmen ssr]. | | 1976 | 139538 |
| the metabolic effects and uptake of ethidium bromide by rat liver mitochondria. | | 1977 | 141908 |
| biochemical study of testosterone in skin: in vivo and in vitro metabolism of testosterone in rat skin. | | 1977 | 143191 |
| variation in potassium transport properties of mouse 3t3 cells as a result of subcultivation. | unindirectional potassium influx and the fraction of this influx sensitive to ouabain, an inhibitor of the (na + k) activated atpase, have been evaluated as a function of subcultivation of the 3t3 and sv40 transformed 3t3 cell. total and ouabain-sensitive k influx change little over approximately 50 passages of the transformed 3t3 cell. in contrast, these components of k influx increase nearly 5-fold over a similar number of passages of the 3t3 cell. during early passages total and ouabain-sens ... | 1977 | 145445 |
| endotoxin receptor site. ii. specificity of endotoxin receptor of platelets and sensitivity to endotoxin in vivo. | the biological specificity of the endotoxin receptor on platelet membranes was examined. the binding indices of platelets in experimental endotoxemia which was induced by intravenous administration of endotoxin (lipopolysaccharide of e. coli, difco) to rabbits were found to be 30% of the control at 60 min after the injection. the result suggests that the endotoxin receptor of platelets was already occupied. the binding indices of human platelets were measured after pretreatment with pharmacologi ... | 1978 | 151487 |
| [regulation of functions of myosin and actomyosin from smooth muscle (author's transl)]. | | 1978 | 152446 |
| adult canine myocytes: isolation, morphology and biochemical characteristics. | | 1979 | 155746 |
| lymphoproliferative disease in a cotton-top marmoset after inoculation with infectious mononucleosis-derived epstein-barr virus. | injection of concentrated ebv derived from cells of the kaplan line of infectious mononucleosis (im) origin resulted in malignant lymphoproliferation in one out of three cotton-top marmosets 6 weeks after inoculation. two additional animals receiving the same isolate after incubation with an antibody-containing human serum did not develop tumors. inoculation of concentrated virus derived from the p3hr-1 line of burkitt origin did not lead to lymphoproliferations in five marmosets. three of these ... | 1975 | 168158 |
| [comparitive study of phosphatase activity in the seminal plasma of various domestic animals in egypt (short communication)]. | | 1975 | 169119 |
| effect of jet aircraft noise upon in vitro adrenocortical response to acth in feral mus musculus. | | 1976 | 176555 |
| use of cryopreserved virus-infected target cells in a lymphocytotoxicity 51cr release microassay for cell-mediated immunity to cytomegalovirus. | a new 51cr-release microassay is described for the measurement of cell-mediated immunity to cytomegalovirus, using cryopreserved target cells acutely infected with cytomegalovirus. | 1976 | 177371 |
| [localization of genome rna synthesis in sendai virus]. | localization of sendai virus 50s rna in ehrlich ascitic carcinoma cells was studied. at 48 hours postinfection virus-specific 50s rna was found in the nucleus after 30 min exposure to 3h-uridine, and its amount increased after 1-hour exposure to the precursor reaching 18% of the total nucleus virus-specific rna. after 2-hour or longer exposure to the precursor the amount of 50s rna in the nuclei decreased considerably and in the cytoplasm in this gradient region a considerable radioactivity appe ... | 1975 | 179216 |
| infectious murine type-c viruses released from human cancer cells transplated into nude mice. | type-c virus particles were revealed by electron microscope in 6 of 9 tumours of cultured and biopsied human cancers heterotransplanted into nude mice. some tumours in nude mice were explanted for in vitro cultivation. the virus particles were also found in the cultures derived from the virus-positive tumors. they were mostly found extracellularly, but the particles in budding process were also encountered frequently. cytological study and karyotype analysis of the cultured cells proved these vi ... | 1977 | 193756 |
| [proviral genetic material of rous sarcoma virus in mammalian tumor cells]. | | 1977 | 195780 |
| isolation of highly purified leydig cells by density gradient centrifugation. | purification of specific endocrine cells from mixed populations after dispersion of target tissues is important for detailed analysis of mechanisms of hormone action. a simple method for rapid isolation of endocrine cells with retention of biological integrity, has been developed by centrifugation in density gradients formed with metrizamide. by this procedure, highly purified leydig cells retaining morphological and biochemical characteristics were obtained. such preparations bound 20, 300+/-3, ... | 1977 | 195801 |
| turnover of carbamyl-phosphate synthase, of other mitochondrial enzymes and of rat tissues. effect of diet and of thyroidectomy. | | 1977 | 195806 |
| mutants of luminous bacteria with an altered control of luciferase synthesis. | arginine is known to increase the luminescence in vivo and in vitro of the marine bacterium beneckea harveyi growing in minimal medium. mutants in which this arginine effect is either diminished, or absent were isolated as bright clones on a minimal medium after n-methyl-n'-nitro-n-nitrosoguanidine mutagenesis. on a minimal medium both with and without added arginine and also on complex medium, these "minimal bright" mutants produce higher levels of luminescence than the wild type both in vivo a ... | 1977 | 195929 |
| an investigation of the homology of guanylate kinase isozymes in mammals and further evidence for multiple guk gene loci. | guanylate kinase in the red cells of 63 different mammalian species was studied by electrophoresis and multiple molecular forms of the enzyme were found in all species. two species were investigated in more detail. using molecular weight estimates as a criterion of homology, the fallow deer and the chinese hamster were found to have isozymes that corresponded to isozyme e, f, and g of man. variation in the guanylate kinase isozymes was detected in a small population of orangutans. results sugges ... | 1977 | 201245 |
| [attempts to establish an immunoprevention of transmissible gastroenteritis (tge) in swine. i. pathogenicity of the b1 strain following serial passages]. | | 1977 | 202115 |
| toxoplasmosis, distemper, and herpesvirus infection in a skunk (mephitis mephitis). | a striped skunk (mephitis mephitis) showing abnormal behavior had histopathologic lesions of toxoplasmosis and canine distemper in addition to intranuclear, eosinophilic inclusions in the reticuloendothelial cells of the spleen, liver and lung. the inclusions, on electron microscopic examination, were compatible with herpesvirus infection. | 1978 | 204802 |