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mutant of b-tropic murine leukemia virus synthesizing an altered polymerase molecule.a nonconditional mutant of b-tropic murine leukemia virus (mulv), defective in polymerase, has been isolated by cloning chronically infected cells. the cell clone containing the mutant produced virus particles which were noninfectious. however, superinfection of the cells by replication-competent xc-negative viruses resulted in the rescue of virus capable of forming plaques in a modified xc test, termed the "complementation plaque assay" (a. rein and r. h. bassin, j. virol. 28:656-660, 1978). an ...197992571
[vasodilator activity of co2 in newly formed subcutaneous gas pockets in rats (proceedings)]. 197992930
chemical structure of the cell wall of mycobacterium tuberculosis var. bovis, strain bcg.bcg cell walls contain approximately 30% free lipids like other mycobacterial cell walls. the insoluble skeleton of the cell wall is made up of two covalently linked polymers, a peptidoglycan and an arabinogalactan mycolate, with which are associated non peptidoglycan amino acids and a glucan. we present data on two structural features: 1. the "non peptidoglycan" amino acids; they form two kinds of compounds: peptide chains which can be solubilized by proteolytic enzymes and a trypsin-chymotryps ...1975126548
proton translocation in cytochrome-deficient mutants of escherichia coli.cytochrome-deficient cells of a strain of escherichia coli lacking 5-amino-levulinate synthetase have been used to study proton translocation associated with the reduced nicotinamide adenine dinucleotide (nadh) dehydrogenase region of the electron transport chain. menadione was used as electron acceptor, and mannitol was used as the substrate for the generation of intracellular nadh. the effects of iron deficiency on nadh- and d-lactate-menadione reductase activities were studied in iron-deficie ...1979154508
temperature-sensitive mutants of herpes simplex virus type 1 defective in transcriptional and post-transcriptional functions required for viral dna synthesis. 1978214940
immune response to a mammary adenocarcinoma. vi. blocking of tumor-specific cell-mediated cytotoxicity by antibodies to the cytotoxic lymphocyte. 1978309904
the terminal oxidase of photobacterium phosphoreum. a novel cytochrome.the terminal oxidase of photobacterium phosphoreum has been purified to the electrophoretically homogeneous state and some of its properties have been studied. the enzyme catalyses oxidation of ascorbate in the presence of phenazine methosulphate or n,n,n',n'-tetramethyl-p-phenylenediamine. the reaction is inhibited by cyanide. nitrite at comparatively high concentrations inhibits the enzyme, but the enzyme does not catalyse nitrite reduction with ascorbate plus the electron mediator as the elec ...1979465487
evidence for a fatty acid reductase catalyzing the synthesis of aldehydes for the bacterial bioluminescent reaction. resolution from luciferase and dependence on fatty acids.the enzyme responsible for the stimulation by atp and nadph of light emission catalyzed by bacterial luciferase has been partially purified from extracts of the luminescent bacterium, photobacterium phosphoreum. the stimulatory activity was found to be stabilized by high concentrations of mercaptoethanol, permitting it to be separated from luciferase into an active and stable form and enabling further characterization of its functional properties. the activity of the enzyme was shown to be depen ...1979572825
studies on luciferase from photobacterium phosphoreum. x. heat of formation of the intermediate in the bioluminescent reaction studied by stopped-flow calorimetry.the heat production in the reaction of luciferase-fmnh2 complex with o2 in the absence of aldehyde was measured by stopped-flow calorimetry. deltah of the reaction, luciferase-fmnh2+ o2 leads to intermediate x1, is -1.3 x 10(2) kj.mol-1 and the calculated deltas for the reaction is -180 j.mol-1.k-1 at 20 degrees c. the heat production in the bioluminescent reaction was also measured in the presence of a saturating concentration of aldehyde, and it was estimated that 43 and 79% of the c10 and c13 ...1978659382
properties and kinetics of salt activation of a membrane-bound nadh dehydrogenase from a marine bacterium photobacterium phosphoreum.a membrane-bound nadh dehydrogenase, solubilized and partially purified from a marine bacterium photobacterium phosphoreum, contains fad as the prosthetic group, and is specific for nadh. ferricyanide, various other redox dyes and cytochrome c can act as electron acceptors. the enzymatic activity when assayed with electron acceptors other than cytochrome c, is activated by monovalent cations (na+ and k+) and deactivated by high concentrations of monovalent anions (scn-, no3-, and cl-) but not by ...1978721793
studies on luciferase from photobacterium phosphoreum. xi. interaction of 8-substituted fmnh2 with luciferase.the interaction of bacterial luciferase from photobacterium phosphoreum with reduced flavin was investigated using various 8-substituted fmnh2 analogs. flavins tested were fmnh2 and fmnh2 substituted at the 8 position with ho-, ch3o-, c2h5o-, cl-, br-, i-, h2n-, (ch3)hn-, and (ch3)2n. 8-ch30-, c2h5o-, cl-, and br-fmnh2 showed luminescent activity in the luciferase reaction with emission peaks at various wavelengths. 8-ho- and i-fmnh2 were competitive inhibitors toward fmnh2 in the luminescent re ...1978738995
studies on luciferase from photobacterium phosphoreum. ix. further studies on the spectroscopic characteristics of the enzyme-fmn intermediates.the absorption and fluorometric changes of the reaction mixture of luciferase-fmnh2 complex with o2 were re-examined. rapid formation (k2(app) = 2.0 s-1 at [o2] = 120 micrometer) of an intermediate with a single absorption maximum at 380 nm within the range of 350-550 nm, and a weak fluorescence at 520 nm (less than or equal to 10% of that of fmn when excited at 380 nm) was observed. the absorption and fluorescence spectra and decay rate of the intermediate were estimated by simulation using an ...1977881410
studies on luciferase from photobacterium phosphoreum. viii. fmn-h2o2 initiated bioluminescence and the thermodynamics of the elementary steps of the luciferase reaction.bacterial luciferase from photobacterium phosohoreum was found to produce bioluminescence on reaction with fmn and h2o2 in the presence of aldehyde. this luminescence is presumably produced by the same x1 intermediate as that found in fmnh2-o2 initiated luminescence. from the ratio of the light intensities of the fmn-h2o2 initiated reactions, we calculated the association constant of the reaction, luciferase+fmn+h2o2in equilibriumx1, and estimated its temperature dependence. from these results w ...1976950335
[the luminescent bacteria test for clean water legislation].luminescent bacteria (photobacterium phosphoreum) may be used, on the one hand, for classical toxicity tests based on the inhibition of respiration or growth and, on the other hand, their luminescence itself may become the test criterion. such a luminescence inhibition test is commonly called a luminescent bacteria test. it may be conducted with fresh bacteria or with conserved ones. the luminescence parameter is, just like the parameter oxygen consumption, a summative parameter for undisturbed ...19921307824
[luminescence, growth and respiration as end points of the toxic effects on photobacterium phosphoreum--short report]. 19921307830
microtox ec50 values for drinking water by-products produced by ozonolysis.the aim was to determine the microtox ec50 values of some aliphatic aldehydes and carboxylic acids of normal chain length with 1-14 carbon atoms since these compounds have been detected as ozonolysis by-products in drinking water. the aqueous ec50 values decreased with increasing chain length except for formaldehyde and for the c1-c7 acids. at chain lengths above c7, where methanolic saline solutions were utilized to promote solubility, the aldehydes were more toxic than their corresponding carb ...19921376239
fluorescence study of the ligand stereospecificity for binding to lumazine protein.6,7-dimethyllumazine derivatives, substituted at the 8-position with aldityls or monohydroxyalkyl groups, have been examined for their binding ability to lumazine apo-protein from two strains of photobacterium phosphoreum using fluorescence dynamics techniques. on the protein the lumazine has a nearly monoexponential decay of fluorescence with lifetime 13.8 ns (20 degrees c). in free solution the lifetime is 9.6 ns. the concentration of free and bound lumazine in an equilibrium mixture can be re ...19921483455
investigations of phagocytosis concerning the immunological defence mechanism of mytilus edulis using a sublethal luminescent bacterial assay (photobacterium phosphoreum).1. a simple method for the determination of phagocytosis activity using mussel hemocytes by measuring the bioluminescence is presented. 2. the immunological defence activity based on phagocytosis is measured and quantified by a luminescent bacterial assay with photobacterium phosphoreum. 3. the measuring system allows us to establish the stress of the immunological defence mechanism of organisms exposed to chemicals and polluted rivers or sewage. results with reference substances and the phagocy ...19911677842
crystallization and preliminary x-ray diffraction studies of a flavoprotein, fp390, from a luminescent bacterium, photobacterium phosphoreum.a flavoprotein, fp390, obtained from a luminescent bacterium, photobacterium phosphoreum, in the purification of luciferase has been crystallized by the vapor-diffusion procedure. crystals obtained from polyethylene glycol 4000 solutions, whose x-ray photographs show powder diffraction patterns, were unsuitable for further crystallographic work. however, tetragonal crystals grown from potassium phosphate solution well diffracted x-rays beyond 3 a resolution. the space group of this crystal is p4 ...19911783606
qsar studies of comparative toxicity in aquatic organisms.this study investigated the relationships between the toxicities of common organic pollutants to the fathead minnow (pimephales promelas), to daphnia magna, to tetrahymena pyriformis and in the microtox test, which uses the luminescent bacterium photobacterium phosphoreum. the toxicity data were compiled from the literature, with the exception of 40 experimentally determined microtox data. encouraging correlations are seen, indicating significant relationships between fish toxicities and those t ...19911815364
regression and cluster analysis of the acute toxicity of 267 chemicals to six species of biota and the octanol/water partition coefficient.the acute toxicities of 267 compounds to six aquatic and one terrestrial species were investigated with correlation, principal component and cluster analysis techniques for relationships with each other and with the compounds' octanol/water partition coefficient. selection of the investigated chemicals was based on the availability of at least three of the following measured parameters: acute (24-h to 96-h) lethal concentrations (lc50) to the fish fathead minnow (pimephales promelas), the fish g ...19911815369
evaluation of the genotoxic activity and acute toxicity of euphorbia splendens latex, a molluscicide for the control of schistosomiasis.1. the latex of euphorbia splendens var. hislopii has a molluscicidal action at low concentration (ld90 less than 1.5 ppm or 1.5 micrograms/ml) against the vector snails of schistosomiasis. 2. in the present study, the latex in natura or after lyophilization was submitted to the ames test and the chromotest to evaluate genotoxicity, to the microtox system to determine acute toxicity, and to the chinese hamster ovary cell assay (cho) to measure cytotoxicity. 3. the latex had no mutagenic activity ...19911823273
astrocytes: a possible primary site for colchicine-mediated neurotoxicity in the rat striatum.ultrastructural changes in rat astrocytes including endfoot swelling and rupture of plasma membranes were seen as early as 1 h after injection of 6.0 micrograms colchicine (co) into the striatum. significant change in neuronal morphology was not seen by 24 h. astrocytes may therefore be primary targets for co-mediated damage, which then may have secondary neurotoxic consequences.19911828783
complexity and sequence identification of 24 rat v beta genes.twenty-four tcr v beta genes were cloned by anchored pcr from the lewis rat strain and identified by nucleotide and amino acid sequence comparisons to known mouse v beta genes. rat v beta genes exist in 17 single-member and 3 multimember subfamilies and exhibit 86 to 94 and 72 to 92% nucleotide and amino acid sequence similarities, respectively, to their mouse counterparts. a single rat gene, designated v beta 20, having no previously known mouse counterpart was identified; a closely related gen ...19911828824
development of species-specific hybridization probes for marine luminous bacteria by using in vitro dna amplification.by using two highly conserved region of the luxa gene as primers, polymerase chain reaction amplification methods were used to prepare species-specific probes against the luciferase gene from four major groups of marine luminous bacteria. laboratory studies with test strains indicated that three of the four probes cross-reacted with themselves and with one or more of the other species at low stringencies but were specific for members of their own species at high stringencies. the fourth probe, g ...19911854194
marine biosurfactants, iii. toxicity testing with marine microorganisms and comparison with synthetic surfactants.eight synthetic and nine biogenetic surfactants were tested on their toxicity. because of their possible application as oil dispersants against oil slicks on sea, the test organisms used were marine microorganisms (mixed and pure cultures of bacteria, microalgae, and protozoa). bacterial growth was hardly effected or stimulated, whilst that of algae and flagellates was reduced. all substances tested were biodegraded in sea water. the bioluminescence of photobacter phosphoreum (microtox test) was ...19911878108
the acute toxicity of pulse-dosed, para-substituted phenols to larval american flagfish (jordanella floridae): a comparison with toxicity to photoluminescent bacteria and predicted toxicity using log kow.the acute toxicity of nine para-substituted phenols was determined using a pulse-exposure testing protocol and 8-day-old larval american flagfish (jordanella floridae). relative tolerance was assessed by determining the 2-h pulse exposure concentration causing 20 and 50% mortality (pe lc20 and pe lc50) over the subsequent 94 h. four bioassays were run for each phenol and yielded the following mean pe lc20 values (mg 1(-1)) in descending order of toxicity: p-aminophenol, 0.06; hydroquinone, 0.13; ...19911891709
borrowed proteins in bacterial bioluminescence.a library of photobacterium phosphoreum dna was screened in lambda 2001 for the lumazine protein gene, using two degenerate 17-mer oligonucleotide probes that were deduced from a partial protein primary sequence. the lumazine protein gene was localized to a 3.4-kilobase bamhi/ecori fragment in one clone. the fragment contained an open reading frame, encoding a 189-residue protein, that had a predicted amino acid sequence that concurred with the partial sequence determined for lumazine protein. c ...19911996310
structure and properties of luciferase from photobacterium phosphoreum.the nucleotide sequences of the luxa and luxb genes coding for the alpha and beta subunits, respectively, of luciferase from photobacterium phosphoreum have been determined. the predicted amino acid sequences of the alpha and beta subunits were shown to be significantly different from other bacterial luciferases with 62 to 88% identity with the alpha subunits and 47 to 71% identity with the beta subunits of other species. expression of the different luciferases appear to correlate with the numbe ...19912018544
identification of the acyl transfer site of fatty acyl-protein synthetase from bioluminescent bacteria.fatty acid activation, transfer, and reduction by the fatty acid reductase multienzyme complex from photobacterium phosphoreum to generate fatty aldehydes for the luminescence reaction is regulated by the interaction of the synthetase and reductase subunits of this complex. identification of the specific site involved in covalent transfer of the fatty acyl group between the sites of activation and reduction on the synthetase and reductase subunits, respectively, is a critical step in understandi ...19912023262
further study on polyamine compositions in vibrionaceae.it has previously been reported that norspermidine, one of the unusual polyamines, is present in vibrio species. to expand this observation, the cellular polyamine compositions of additional species and strains in the family vibrionaceae (vibrio, photobacterium, listonella, and shewanella) as well as aeromonas species and plesiomonas shigelloides, which have been proposed to be excluded from vibrionacea, were determined by using gas-liquid chromatography. some vibrio species previously reported ...19912059921
electronic excitation transfer in the complex of lumazine protein with bacterial bioluminescence intermediates.fluorescence dynamics measurements have been made on the bioluminescence reaction intermediates using photobacterium leiognathi, vibrio fischeri, and vibrio harveyi luciferases, both alone and in mixtures with photobacterium phosphoreum lumazine protein. each luciferase produces a "fluorescent transient" intermediate on reaction with the bioluminescence substrates, fmnh2, tetradecanal, and o2, and all have a fluorescence quantum yield about 0.3, with a predominant lifetime around 10 ns. the p. l ...19912069948
a lux-specific myristoyl transferase in luminescent bacteria related to eukaryotic serine esterases.the diversion of fatty acids from fatty acid biosynthesis into the luminescent system is catalyzed by a lux-specific acyltransferase that catalyzes the cleavage of fatty acyl-acyl carrier protein (acp). analysis of the substrate specificities for fatty acyl-acps of the transferases from divergent luminescent bacteria, photobacterium phosphoreum and vibrio harveyi, has demonstrated that myristoyl-acp is cleaved at the highest rate. inhibition by phenylmethanesulfonyl fluoride as well as resistanc ...19912071574
affinity purification of bacterial luciferase and nad(p)h:fmn oxidoreductases by fmn-sepharose for analytical applications.a modified purification method for bacterial luciferases and nad(p)h:fmn oxidoreductases is described which uses fmn-sepharose alone or coupled to deae ion exchange chromatography for the simultaneous purification of luciferase and the various oxidoreductases from vibrio harveyi, a bright mutant of vibrio harveyi, vibrio fischeri, and photobacterium phosphoreum. this purification method is compared with deae-sepharose cl 6b fractionations from these organisms. both methods allow the separation o ...19902220416
a soluble fatty acyl-acyl carrier protein synthetase from the bioluminescent bacterium vibrio harveyi.an enzyme catalyzing the ligation of long chain fatty acids to bacterial acyl carrier protein (acp) has been detected and partially characterized in cell extracts of the bioluminescent bacterium vibrio harveyi. acyl-acp synthetase activity (optimal ph 7.5-8.0) required millimolar concentrations of atp and mg2+ and was slightly activated by ca2+, but was inhibited at high ionic strength and by triton x-100. acp from either escherichia coli (apparent km = 20 microm) or v. harveyi was used as a sub ...19902223012
the lumazine protein gene in photobacterium phosphoreum is linked to the lux operon. 19902243804
bacterial luminescence: a new tool for investigating the effects of acoustic energy and cavitation.an assay utilizing luminescent bacteria, photobacterium phosphoreum, was adapted to assess the antibacterial effects of acoustic energy. acoustic pressures up to 67 kpa in the 100- to 800-hz frequency range were applied to bacteria freely suspended in a liquid medium. bacterial luminescence decreased after sonication, thus showing sensitivity to the effects of acoustic energy. this decreased luminescence was linearly related to exposure duration, appeared independent of acoustic frequency in thi ...19902283427
13c and 15n nmr studies on the interaction between 6,7-dimethyl-8-ribityllumazine and lumazine protein.the interaction between the prosthetic group 6,7-dimethyl-8-(1'-d-ribityl)lumazine and the lumazine apoproteins from two marine bioluminescent bacteria, one from a relatively thermophilic species, photobacterium leiognathi, and the other from a psychrophilic species, photobacterium phosphoreum, was studied by 13c and 15n nmr using various selectively enriched derivatives. it is shown that the electron distribution in the protein-bound 6,7-dimethyl-8-ribityllumazine differs from that of free 6,7- ...19902331466
inhibition of bioluminescence in photobacterium phosphoreum by sulfamethizole and its stimulation by thymine.in bioluminescent bacteria very few agents have been reported that can selectively inhibit the luminescence. in sensitivity tests with photobacterium phosphoreum, using 55 different antibiotics, it was found that sulfamethizole, an inhibitor of dihydropteroate synthetase and the formation of folic acid, inhibited bioluminescence more than growth. likewise, in mutants requiring thymine for growth, the luminescence per cell was much less in a medium low in thymine. in neither case could the decrea ...19902372557
elongation of exogenous fatty acids by the bioluminescent bacterium vibrio harveyi.bioluminescent bacteria require myristic acid (c14:0) to produce the myristaldehyde substrate of the light-emitting luciferase reaction. since both endogenous and exogenous c14:0 can be used for this purpose, the metabolism of exogenous fatty acids by luminescent bacteria has been investigated. both vibrio harveyi and vibrio fischeri incorporated label from [1-14c]myristic acid (c14:0) into phospholipid acyl chains as well as into co2. in contrast, photobacterium phosphoreum did not exhibit phos ...19892492504
[specific properties of dna from fractions of isolated mouse synaptonemal complexes]. 19892598773
[specific properties of dna from fractions of isolated mouse synaptonemal complexes]. 19892598773
evolution of a trna operon in gamma purple bacteria.genomic dna from eubacteria belonging to the gamma-3 subdivision of purple bacteria, as classified by woese (c.r. woese, microbiol. rev. 51:221-271, 1987), were probed with the argt operon of escherichia coli encoding 5'-trna(arg)-trna(his)-trna(leu)-trna(pro)-3'. the homologous operon from vibrio harveyi was isolated and sequenced. comparison of the five available sequences of this trna cluster from members of the families enterobacteriaceae, aeromonadaceae, and vibrionaceae led to the conclusi ...19892687235
dynamic fluorescence study of the interaction of lumazine protein with bacterial luciferases.the equilibrium association of lumazine protein from photobacterium phosphoreum with luciferases from either p. phosphoreum or an aldehyde-requiring dark mutant of vibrio harveyi is measured from changes of the rotational correlation time which is derived from the decay of the lumazine ligand's fluorescence anisotropy. the rotational correlation time of lumazine protein is 23 ns (2 degrees c, 0.25 m pi) and is increased on addition of luciferase due to the formation of a higher molecular weight ...19892720095
interaction between luciferases from various species of bioluminescent bacteria and the yellow fluorescent protein of vibrio fischeri strain y-1.the interaction of the yellow fluorescent protein (yfp) from vibrio fischeri strain y-1 with luciferases from other bioluminescent bacterial strains have been studied. addition of purified yfp to a y-1 luciferase assay results in enhancement of the intensity of blue (484 nm) bioluminescence and a new peak in the emission spectrum at about 540 nm. the bimodal spectrum also resulted when the luciferase used in the reaction was isolated from the species v. fischeri atcc 7744, v. fischeri y-1, photo ...19892742584
covalent reaction of cerulenin at the active site of acyl-coa reductase of photobacterium phosphoreum.inhibition of bioluminescence in photobacterium phosphoreum by cerulenin has been demonstrated to be due to a specific inactivation of the acyl-coa reductase subunit of the fatty acid reductase complex required for synthesis of the aldehyde substrate for the luminescent reaction. in contrast, the activities of the other luminescence-related enzymes, acyl-protein synthetase, acyl-transferase, and luciferase, were unaffected by cerulenin. myristoyl-coa, but not nadph, protected the acyl-coa reduct ...19892751874
bioluminescence spectral and fluorescence dynamics study of the interaction of lumazine protein with the intermediates of bacterial luciferase bioluminescence.the mechanism of the shifting of the bioluminescence spectrum from the reaction of bacterial luciferase by lumazine protein is investigated by methods of fluorescence dynamics. a metastable intermediate is produced on reaction of vibrio harveyi luciferase with fmnh2 and o2. it has an absorption maximum at 374 nm and a rotational correlation time (phi) derived from the decay of its fluorescence (maximum 500 nm) anisotropy of 90 ns (2 degrees c). lumazine protein from photobacterium phosphoreum ha ...19892765486
time-resolved fluorescence spectroscopy of lumazine protein from photobacterium phosphoreum using synchrotron radiation.time-resolved fluorescence on lumazine protein from photobacterium phosphoreum was performed with synchrotron radiation as a source of continuously tunable excitation. the experiments yielded structural and dynamic details from which two aspects became apparent. from fluorescence anisotropy decay monitoring of lumazine fluorescence with different excitation wavelengths, the average correlation times were shown to change, which must indicate the presence of anistropic motion of the protein. a sim ...19892767000
transcription from a plant gene promoter in animal cells.the promoter segment of a plant gene (maize alcohol dehydrogenase 1 (adh 1)) has been fused to two bacterial reporter genes, ecogpt (1) and neo (2), in psv2-derived vectors and introduced into cultured mammalian cells by dna transfection. the padh1-gpt plasmids transformed the recipient cells for resistance to mycophenolic acid plus xanthine (3) and the analogous padh1-neo plasmid transformed cells to g418 resistance (2). s1 analysis of transient transfections of cv1 cells with various derivativ ...19852866489
regulation and properties of the glutamine synthetase purified from photobacterium phosphoreum.glutamine synthetase from a marine enterobacterium, photobacterium phosphoreum, was purified to homogeneity from cells grown in glycerol-yeast extract medium. the purified enzyme had a molecular weight of approximately 670,000 and a subunit size of 56,000, i.e. larger than that of the enzyme from e. coli. regulation of the glutamine synthetase activity by adenylylation/deadenylylation was demonstrated on snake venom phosphodiesterase treatment. the state of adenylylation appeared to influence bo ...19862870058
importance of pulmonary blood volume and aortic blood pressure in regulation of left ventricular function. 19882900297
[central neurotransmitters and control of specific appetite for the macronutrients].studies of brain monoamines and neuropeptides have provided extensive evidence in support of their role in the control of food intake, meal patterns and appetite for specific macronutrients. in this process, the medial and lateral portions of the hypothalamus have a critical responsibility in balancing signals for hunger and satiety. via its rich and biologically active neurotransmitter substances, the hypothalamus monitors and integrates the complex sensory and metabolic input concerning the nu ...19882901820
immunological investigation of the distribution of cytochromes related to the two terminal oxidases of escherichia coli in other gram-negative bacteria.monospecific antibodies were raised against the two terminal oxidase complexes of the aerobic respiratory chain of escherichia coli. these are the cytochrome d and cytochrome o complexes. the antibodies were used to check for the occurrence of cross-reactive antigens in membrane preparations from a variety of gram-negative bacteria by rocket immunoelectrophoresis and immunoblotting techniques. with these criteria, proteins closely related to the cytochrome d complex of e. coli appeared to be wid ...19852981822
purification and properties of a cytochrome b560-d complex, a terminal oxidase of the aerobic respiratory chain of photobacterium phosphoreum.a cytochrome b560-d complex, a terminal oxidase in the respiratory chain of photobacterium phosphoreum grown under aerobic conditions, was purified to near homogeneity. the purified oxidase complex is composed of equimolar amounts of two polypeptides with molecular weights of 41,000 and 54,000, as determined by gel electrophoresis in the presence of sodium dodecyl sulfate. it contains 10.2 nmol of protoheme and 22.5 nmol of iron/mg of protein. the enzyme is a "cytochrome bd-type oxidase," showin ...19863011768
cloning and expression of the photobacterium phosphoreum luminescence system demonstrates a unique lux gene organization.the organization of the lux structural genes (a-e) in photobacterium phosphoreum has been determined and a new gene designated as luxf discovered. the p. phosphoreum luminescence system was cloned into escherichia coli using a pbr322 vector and identified by cross-hybridization with vibrio fischeri lux dna. the lux genes were located by specific expression of p. phosphoreum dna fragments in the t7-phage polymerase/promoter system in e. coli and identification of the labeled polypeptide products. ...19883049575
highly repetitive trna(pro)-trna(his) gene cluster from photobacterium phosphoreum.a dna fragment comprising the four trna gene sequences of the escherichia coli argt locus hybridized with two sau3a-generated dna fragments from the vibrio photobacterium phosphoreum (atcc 11040). detailed sequence analysis of the longer fragment shows the following gene organization: 5'-promoter-trna(pro)-trnapro-trna(pro)-trna(his)-trna(pro)-trna(pro)- trna(his)-trna(pro)-five pseudogenes derived from the upstream trnapro interspersed by putative rho-independent terminators. this sequence demo ...19883056906
interaction of aflatoxin b1 and cyclopiazonic acid toxicities.toxic properties of the mycotoxins cyclopiazonic acid and aflatoxin b1 have been analyzed separately and in combination by monitoring their effects on luminescence in the marine bacterium photobacterium phosphoreum, strain ncmb 844. genotoxicity was analyzed with a dark mutant of this organism whose reversion to the bioluminescent condition is stimulated by compounds attacking guanine sites in deoxyribonucleic acids. in this assay, cyclopiazonic acid, unlike aflatoxin b1, is not enhanced by cycl ...19873130566
inhibition of feline infectious peritonitis virus replication by recombinant human leukocyte (alpha) interferon and feline fibroblastic (beta) interferon.replication of feline infectious peritonitis virus (fipv) in feline cell cultures was inhibited after incubation of cells with either human recombinant leukocyte (alpha) interferon (ifn) or feline fibroblastic (beta) ifn for 18 to 24 hours before viral challenge exposure. compared with virus control cultures, fipv yields were reduced by ranges of 0.1 to 2.7 log10 or 2 to 5.2 log10 tcid50 in cultures treated with human alpha- or feline beta-ifn, respectively; yield reductions were ifn dose depend ...19883178028
tandemly repeated trna pseudogenes in photobacterium.a region distal to three trna genes in photobacterium phosphoreum, a gram-negative eubacterium, unexpectedly contains a high number of repeated dna segments that are closely related to the adjacent trnapro gene. the 5' to 3' order of this cluster is trnapro-trnahis-trnapro followed by eight trnapro-like structures interspersed by rho-independent terminators. the two trnapro genes, which are identical, and the trnahis gene have 86% and 87% positional identity, respectively, to their counterparts ...19883194413
a new lux gene in bioluminescent bacteria codes for a protein homologous to the bacterial luciferase subunits.the nucleotide sequence of a new gene, luxf, located between the luxb and e genes in the bioluminescent system of photobacterium phosphoreum has been determined. the luxf gene codes for a polypeptide of 231 amino acids which is homologous to the alpha and beta subunits of luciferase coded by the luxa and luxb genes, respectively. the degree of homology of the luxf protein is very high with the beta subunit of luciferase (approximately 30% identity) with greatest similarity to the vibrio luxb pro ...19883415691
lactic acidosis during closed-chest cpr in dogs.survival after out-of-hospital cardiac arrest is intimately related to the time from cardiovascular collapse to the initiation of cpr, or downtime. furthermore, the reperfusion technique that optimizes coronary and cerebral blood flow after cardiac arrest may also be dependent on downtime. peak blood lactate levels have been shown to be unchanged throughout resuscitation and predictive of downtime in dogs subjected to cardiopulmonary arrest and open cardiac massage. the purpose of this study was ...19873688590
intersubunit transfer of fatty acyl groups during fatty acid reduction.fatty acid reduction in photobacterium phosphoreum is catalyzed in a coupled reaction by two enzymes: acyl-protein synthetase, which activates fatty acids (+atp), and a reductase, which reduces activated fatty acids (+nadph) to aldehyde. although the synthetase and reductase can be acylated with fatty acid (+atp) and acyl-coa, respectively, evidence for acyl transfer between these proteins has not yet been obtained. experimental conditions have now been developed to increase significantly (5-30- ...19863782102
evaluation of a new approach to the safety assessment of biomaterials.the effectiveness of a bacterial luminescence inhibition assay in assessing the toxicity of compounds which are released from biomaterials was evaluated. luminescence from a strain of bacteria most closely resembling photobacterium phosphoreum was measured. the concentration that inhibited luminescence by 50% (ec50) was determined for selected plasticizers, monomers and additives. the intraperitoneal (i.p.-ald) and intravenous (i.v.-ald) approximate lethal doses were determined using mice. by ra ...19863816615
interleukin 1 released from beagle alveolar macrophages exposed to dust particles. 19853877837
selenium mediated reduction of the toxicity expression of cigarette smoke condensate in photobacterium phosphoreum. 19863947767
fatty acyl-amp as an intermediate in fatty acid reduction to aldehyde in luminescent bacteria.the acyl protein synthetase component (50k) of the fatty acid reductase complex from the luminescent system of photobacterium phosphoreum has been found to catalyze the activation of fatty acid via formation of an enzyme bound acyl-amp (carboxyphosphate mixed anhydride) immediately prior to the acylation of the enzyme. ppi-atp exchange and nucleotide binding experiments are dependent on fatty acid and indicate that the fatty acyl-amp is directly formed and that an adenylated enzyme intermediate ...19853968067
purification of lumazine proteins from photobacterium leiognathi and photobacterium phosphoreum: bioluminescence properties.bright strains of the marine bioluminescent bacterium photobacterium leiognathi produce a "lumazine protein" in amounts comparable to that previously found in photobacterium phosphoreum. new protocols are developed for the purification to homogeneity of the proteins from both species in yields up to 60%. in dimmer strains the amounts of lumazine protein in extracts are less, and also there is an accompanying shift of the bioluminescence spectral maximum to longer wavelength, 492 nm. both types o ...19853986184
chemical characterization of lumazine protein from photobacterium leiognathi: comparison with lumazine protein from photobacterium phosphoreum.the properties of lumazine proteins purified from the marine bioluminescent bacteria photobacterium phosphoreum, a psychrophile, and photobacterium leiognathi, a relatively thermophilic species, are compared. an accurate 1:1 stoichiometry of binding of the ligand 6,7-dimethyl-8-ribityllumazine to each lumazine protein is established by back-titration of the apoprotein with the authentic ligand, using both fluorescence and absorption measurements. neither protein contains metal cofactors, organic ...19853986185
spectral properties and function of two lumazine proteins from photobacterium.the spectral properties are compared for two 6,7-dimethyl-8-ribityllumazine proteins from marine bioluminescent bacteria, one from a psychrophile, photobacterium phosphoreum, and the other from a thermophile, photobacterium leiognathi. the visible spectral properties, which are the ones by which the protein performs its biological function of bioluminescence emission, are almost the same for the two proteins: at 2 degrees c and 50 mm pi, ph 7, fluorescence quantum yield phi f = 0.59 and 0.54, re ...19853986186
physical characterization of lumazine proteins from photobacterium.the physicochemical properties of photobacterium lumazine proteins have been investigated. the molecular weights obtained by several physical techniques are in good agreement, and the averages are 2% and 8% higher than the minimum molecular weights from amino acid and ligand content. the average molecular weights, sedimentation coefficients, and molecular radii are respectively the following: photobacterium leiognathi lumazine protein, 21 200 +/- 300, 2.18 s, and 22.9 a; photobacterium phosphore ...19853986187
quantitative structure-activity relationships and mixture toxicity of organic chemicals in photobacterium phosphoreum: the microtox test.quantitative structure-activity relationships were calculated for the inhibition of bioluminescence of photobacterium phosphoreum by 22 nonreactive organic chemicals. the inhibition was measured using the microtox test and correlated with the partition coefficient between n-octanol and water (poct), molar refractivity (mr), and molar volume (mw/d). at log poct less than 1 and greater than 3, deviations from linearity were observed. introduction of mr and mw/d improved the quality of the relation ...19853987587
direct complement fixation and isolation attempts for detecting chlamydia psittaci infection of psittacine birds.serum and fecal specimens from 147 birds were tested for chlamydia psittaci. isolation rates ranged from 3.4% of 88 birds that had titers less than 8, to 50% of six birds that had titers greater than or equal to 128. testing of 44 paired sera revealed a significant titer increase in 5 pairs, a significant titer decrease in 8 pairs, and a stable titer in 31 pairs. the examination of both feces and serum is recommended for determining chlamydia psittaci infections in individual live psittacine bir ...19854074254
[radioprotective effect of mexamine on oral administration to monkeys]. 19734202792
[animal experiments on the effect of vitamin a on walker carcinoma of the rat]. 19734348296
[animal experiments on the effect of vitamin a on walker carcinoma of the rat]. 19734348296
virus infections of horses at newmarket, 1972 and 1973. 19744375339
action of insulin and triiodothyronine on energy-controlled pathways of hydrogen. 19684387276
cerebral blood flow following induced subarachnoid hemorrhage in the monkey. 19724403562
studies on luciferase from photobacterium phosphoreum. vi. stoichiometry and mode of binding of fmnh2 and o2 to stripped luciferase. 19744426891
[pathogenetic and immunotherapeutic significance of a cutaneous burn toxin in experimental pseudomonas infection]. 19744436111
studies on luciferase from photobacterium phosphoreum. vii. interaction with carboxylic acid. 19744452671
plasma insulin levels, and insulin sensitivity in adrenalectomized rats. effects of corticosterone and dexamethasone. 19744460293
the aldehyde content of luminous bacteria and of an "aldehydeless" dark mutant.fatty aldehydes, present in the luminescent cells of photobacterium phosphoreum and achromobacter fischeri, and to a very slight extent in the cells of a visually dark, "aldehydeless" mutant of the latter species, were extracted, purified, and oxidized to the corresponding acids. the acids were analyzed by mass spectrometry. the results, in conjunction with various other lines of evidence, indicate that saturated fatty aldehydes, comprising mostly dodecanal, tetradecanal, and hexadecanal, functi ...19744531008
studies on luciferase from photobacterium phosphoreum. ii. substrate specificity and stoichiometry of the reaction in vitro. 19724634973
studies on luciferase from photobacterium phosphoreum. 3. isolation and partial characterization of an enzyme-bound pigment. 19724644322
isolation of d-erythro-neopterin 2':3'-cyclic phosphate from photobacterium phosphoreum. 19734699996
studies on luciferase from photobacterium phosphoreum. iv. preparation and properties of stripped luciferase. 19734770374
studies on luciferase form photobacterium phosphoreum. v. an enzyme-fmn intermediate complex in the bioluminescent reaction. 19744834652
status of zinc in cow's milk. 19674962019
on the catabolism of deoxyribonucleosides in cells and cell extracts of escherichia coli. 19684973225
optimal environmental conditions and nutrient concentrations for the synthesis of bacterial luciferase in photobacterium phosphoreum. 19725078563
studies on luciferase from photobacterium phosphoreum. i. purification and physiochemical properties. 19715562345
generation of fatty acids by an acyl esterase in the bioluminescent system of photobacterium phosphoreum.the fatty acid reductase complex from photobacterium phosphoreum has been discovered to have a long chain ester hydrolase activity associated with the 34k protein component of the complex. this protein has been resolved from the other components (50k and 58k) of the fatty acid reductase complex with a purity of greater than 95% and found to catalyze the transfer of acyl groups from acyl-coa primarily to thiol acceptors with a low level of transfer to glycerol and water. addition of the 50k prote ...19846147345
proteolytic inactivation of luciferases from three species of luminous marine bacteria, beneckea harveyi, photobacterium fischeri, and photobacterium phosphoreum: evidence of a conserved structural feature.upon limited proteolysis of luciferases from the luminous marine bacteria photobacterium fischeri, photobacterium phosphoreum, and beneckea harveyi, the rate of loss of luciferase activity is the same as the rate of loss of the heavier subunit of all three enzymes. it thus appears that the larger subunit of the luciferase from p. phosphoreum should be designated alpha based on its apparent homology with the alpha subunits of the luciferases from b. harveyi and p. fischeri. the luciferase from b. ...19806161366
[continuous cultivation of photobacterium phosphoreum luminescent bacteria with control of the luminescence].the paper describes a procedure for continuous cultivation of luminescent bacteria photobacterium phosphoreum according to which the nutrient flow is controlled with respect to the luminescence intensity. the biomass yield of this cultivation procedure at the given luminescence intensity 6.1 x 10(12) quantum x ml-1 s-1 is over three times higher than that obtained in periodic cultivation, with the specific cell luminescence being identical in both cases. the cultivation process is unstable at th ...19836353408
relationship between ion requirements for respiration and membrane transport in a marine bacterium.intact cells of the marine bacterium alteromonas haloplanktis 214 oxidized nadh, added to the suspending medium, by a process which was stimulated by na+ or li+ but not k+. toluene-treated cells oxidized nadh at three times the rate of untreated cells by a mechanism activated by na+ but not by li+ or k+. in the latter reaction, k+ spared the requirement for na+. intact cells of a. haloplanktis oxidized ethanol by a mechanism stimulated by either na+ or li+. the uptake of alpha-aminoisobutyric ac ...19846690427
differential acylation in vitro with tetradecanoyl coenzyme a and tetradecanoic acid (+atp) of three polypeptides shown to have induced synthesis in photobacterium phosphoreum.acylation of extracts of photobacterium phosphoreum at different stages of growth with [3h]tetradecanoic acid (+atp) has shown that two polypeptides found in the fatty acid reductase complex, the fatty acid activating enzyme (50k) and the 34k polypeptide, were specifically labeled during induction of the luminescent system. an alternate method for in vitro acylation of polypeptides in the luminescent system was developed using tetradecanoyl-coa. both the 34k polypeptide and, to a lesser extent, ...19846693412
vibrio harveyi aldehyde dehydrogenase. partial reversal of aldehyde oxidation and its possible role in the reduction of fatty acids for the bioluminescence reaction.vibrio harveyi aldehyde dehydrogenase, which catalyzes the oxidation of long chain aliphatic aldehydes to acids, has been discovered to have both acyl-coa reductase and thioesterase activities. tetradecanoyl-coa was reduced to tetradecanal in the presence of nad(p)h, as monitored by the stimulation of luciferase activity by the aldehyde product (acyl-coa reductase). in the absence of nadph, [3h]tetradecanoyl-coa was hydrolyzed to the hexane-soluble fatty acid (thioesterase). inhibition data with ...19846725283
the effects of phosphate on the structure and stability of the luciferases from beneckea harveyi, photobacterium fischeri, and photobacterium phosphoreum. 19806967319
complementation of subunits from different bacterial luciferases. evidence for the role of the beta subunit in the bioluminescent mechanism.complementation of the nonidentical subunits (alpha and beta) of luciferases isolated from two different bioluminescent strains, beneckea harveyi and photobacterium phosphoreum, has resulted in the formation of a functional hybrid luciferase (alpha h beta p) containing the alpha subunit from b. harveyi luciferase (alpha h) and the beta subunit from p. phosphoreum luciferase (beta p). the complementation was unidirectional; activity could not be restored by complementing the alpha subunit of p. p ...19806969259
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