cytotoxic macrophages induced in hamsters by vaccinia virus: selective cytotoxicity for virus-infected targets by macrophages collected late after immunization. 197987476
long term effects of myochrysine in articular cartilage.intra-articularly administered sodium aurothiomalate (myochrysine) produced aurosomes containing characteristic electron dense contents (indicating the presence of gold), in the chondrocytes of rabbit articular cartilage. at first the aurosomes were bounded by a membrane but later the electron dense contents were seen lying free in the cytoplasmic matrix. such deposits were detectable up to 14 months after injection of myochrysine but none were found at later time intervals (18 months and 2 year ...197897856
clinical studies on pipemidic acid. 1978101691
transformation of horse skin cells by type-c sarcoma viruses.a horse skin cell line (e. derm, nbl-6, ccl-57) was susceptible to focus formation by the kirsten mouse sarcoma virus, feline sarcoma virus (st stain) and the msv pseudotypes with woolly monkey, gibbon monkey, rd-114, at-124, baboon placenta and murine xenotropic (balb/c 3t3 and c57l/jd) type-c viruses. foci were detected within 5 days after infection and the transformed cells continued to produce infectious virus and group-specific antigen of their respective type-c leukemia viruses. the transf ...1975165152
demonstration of oncogenic potential of mammalian cells transformed by dna-containing viruses following photodynamic inactivation.the oncogenic properties of hamster embryo cells transformed by herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) and sv40 virus following photodynamic inactivation using neutral red were determined by subcutaneous inoculation into newborn syrian hamsters. cells transformed by all three viruses produced palpable tumors after different latent periods. histopathological examination showed that hsv-2 tumors were fibrosarcomas and metastases were often seen in the lungs. hsv-2 primary tumors we ...1975165153
preparation of acid-fast microscopy smears for proficiency testing and quality control.a method is presented for preparing smears for proficiency testing and quality control in acid-fast microscopy. the work was prompted by the increased demand for acid-fast bacilli positive smears with characteristic microscopic appearance and among-smear uniformity.1978353070
[bilirubin in the blood serum of clinically healthy cows and those ill with ketosis and hepatopathies].studied was the bilirubin equilibrium in 62 normal cows, 52 cows with clinical ketosis, 31 cows with diffuse liver injury, and 14 cows that went off food for five days. a moderate rise of the total bilirubin of mixed type was found in ketosis-affected cows. statistically significant proved the changes in the free fraction, however, there were no persuasive data that they were due to superproduction of bilirubin. changes in the total bilirubin similar to those in cows with ketosis were establishe ...1979516442
cp-20,961: a structurally novel, synthetic adjuvant. 1979522084
[a strain of aeromonas veronii isolated from a patient suspected of cholera].investigations were carried on a strain isolated from patient hospitalized in poland. the patient returned from odessa, a region with cholera epidemic. the strain was suspected to be vibrio cholerae, but it did not agglutinate in v. cholerae 01 serum. the aim of this study was establishment of taxonomical classification of this strain, in order to demonstrate whether it is a representative of the non-cholera vibrio group (not agglutinating) or another representative of the family vibrionaceae. t ...19921297030
antigenic diversity of the s-layer proteins from pathogenic strains of aeromonas hydrophila and aeromonas veronii biotype sobria.the antigenic relatedness of paracrystalline surface array proteins with subunit molecular weights of approximately 52,000 from isolates of aeromonas hydrophila and aeromonas veronii biotype sobria belonging to a single heat-stable serogroup was examined. enzyme-linked immunosorbent assay and immunoblotting with two different polyclonal antisera against surface exposed and non-surface-exposed epitopes of the s-layer protein from a. hydrophila tf7 showed that the s-layer proteins of the mesophili ...19921370287
immunochemical analysis and possible biological role of an aeromonas hydrophila surface array protein in septicaemia.the biochemical, immunological, and biological properties of an s layer purified from an aeromonas hydrophila strain (ah-342) involved in a case of bacteraemia were investigated. the s layer selectively removed from the cell surface was composed of a single acidic (pi 4.56) protein subunit (surface array protein, sap) with a molecular mass of approximately 52 kda. amino acid analysis of this 52 kda protein indicated a molecule composed of 498 amino acids with 46% hydrophobic residues. no cystein ...19921382113
developmental cycle-specific host-free rna synthesis in chlamydia spp.the incorporation of radiolabeled gtp into rna in host-free chlamydia trachomatis serovar l2 organisms was investigated. the incorporation was partially inhibited by rifampin and dactinomycin and hydrolyzed by rnase. rna made by host-free chlamydiae consisted mainly of species of fewer than 800 bases in size, although 16s and 23s species were noted by agarose-gel electrophoresis. the hybridization of radiolabeled host-free rna to restriction fragments of the gene encoding the major outer membran ...19901698176
monoclonal antibodies to quebec strain (q17) of bovine rotavirus.fourteen monoclonal antibodies (mabs) against "quebec" strain (q17) of bovine rotavirus were isolated and characterized. four were specific for viral protein vp7 and ten were specific for viral protein vp6. five different isotypes were represented by this group of antibodies. all of the anti-vp6 and none of the anti-vp7 antibodies were sensitive to the effects of periodate on their antigen. the antibodies could be separated into three groups based on their relative resistance to the dissociation ...19901698323
biochemical and genetic characterization of autoagglutinating phenotypes of aeromonas species associated with invasive and noninvasive disease.the genetic characteristics and biochemical and structural properties of a number of autoagglutinating (aa) strains of aeromonas associated with invasive and noninvasive disease in humans and infections in animals and from environmental sources were investigated. of 27 strains analyzed by multilocus enzyme typing and dna hybridization studies, 25 (93%) were confirmed to belong to either hybridization group 1 (phenospecies and genospecies aeromonas hydrophila) or 8 (phenospecies aeromonas sobria; ...19912010642
aeromonas jandaei and aeromonas veronii dual infection of a human wound following aquatic exposure.exudate removed from an infection that developed below the left eye of a 10-year-old male following a previously inflicted wound after aquatic exposure was cultured and revealed two different aeromonas spp. further characterization showed that one strain was phenotypically identical to aeromonas veronii, while the other strain was confirmed by dna hybridization analysis to be aeromonas jandaei sp. nov. this is the first report of these more recently described aeromonads, thus far rarely reported ...19912037674
production of enterotoxin and cytotoxin by aeromonas veronii.fourteen strains of aeromonas veronii were isolated from salt and fresh water in rio de janeiro and investigated for the presence of virulence factors. eleven (79%) a. veronii strains were positive for enterotoxin by the suckling-mouse test and thirteen (93%) produced hemolysin. of the 13 a. veronii strains that produced hemolysin, seven were investigated for cytotoxin production using vero cells as indicator cells and all of them were positive.19902095293
serially etched shark enameloid observed by incident light microscopy.longitudinal and transverse tooth sections of isurus oxyrinchus were serially etched in 2.6% nitric acid. the changing optical properties of the etched surfaces were observed during the serial etchings, and the descent of the enameloid surfaces was measured. shark enameloid seems to be less effectively etched by acid than human enamel; this difference may be due to differences in solubility between fluorapatite and hydroxyapatite. most of the information regarding the structure of the enameloid ...1979525241
[yersiniosis]. 19892752462
analysis of the antagonistic effect of gamma-l-glutamyl-taurine on glucocorticoid and triiodothyronine.gamma-l-glutamyl-taurine, a newly discovered parathyroid hormone, considerably inhibited the cytochemically detected transcription-inducing effect (appearance of free histone) of prednisolone and triiodothyronine. such an inhibition took place also if the morphogenetic hormones were given not simultaneously with, but after the administration of litoralon. litoralon injected alone also displayed inductor properties increasing the number of nuclei containing free histone.1979525237
k+, mg2+-aspartate (kmg-asp)--mediated prevention of isoproterenol(iso)-induced metabolic changes in the myocardium.isoproterenol (iso) in a single dose of 7.5 mg/kg b.w. i.v was found to produce alterations of cardiac metabolism in dogs. after 2 h, high energy phosphate stores and glycogen were reduced, whereas the levels of lactate, pyruvate, the lactate/pyruvate ratio and myofibrillar atpase activity were elevated. ca2+ -accumulation by sarcoplasmic reticulum (sr) and mitochondria were increased (p less than 0.01). after 24 h, a partial recovery in the parameters followed could be observed. only myofibrill ...1975126687
[evolution and transformation in animal organism of some polychlorobiphenyls (author's transl)].intoxication by pure polychlorobiphenyls and a commercial product is studied, in rats, after intraperitoneal and oral administration. their fixation and excretion of their metabolites is greater by intraperitoneal than by oral administration. the only hydroxy metabolites, identified by means of synthesis, gas chromatography and mass spectrometry are, all, derivatives from trichlorobiphenyl. it appears that administration of commercial product induces a more rapid degradation of these compounds i ...1978105430
comparison of cefazolin, cefamandole, vancomycin, and ly146032 for prophylaxis of experimental staphylococcus epidermidis endocarditis.we evaluated antibiotic prophylaxis in the rabbit model of experimental endocarditis with three strains of staphylococcus epidermidis of differing susceptibility patterns. for the first strain, which was highly susceptible to methicillin and cephalosporins, vegetations grew s. epidermidis for all 15 untreated rabbits compared with 1 of 20 rabbits receiving cefazolin, 3 of 20 receiving cefamandole, none of 20 receiving vancomycin, and none of 20 receiving ly146032. for the second strain, which wa ...19882831815
mechanism of the epsp synthase catalyzed reaction: evidence for the lack of a covalent carboxyvinyl intermediate in order to detect covalent reaction intermediates in the 5-enolpyruvylshikimate-3-phosphate (epsp) synthase reaction, we have investigated the interaction of epsp synthase with the reaction product epsp. an exchange of epsp-methylene protons could be demonstrated by incubating epsps with epsp in d2o. since trace amounts of contaminating pi would lead to reversal of epsps reaction and hence methylene proton exchange, we added pyruvate kinase, adp, mg++ and k+. under these conditions, any contami ...19882838023
influence of immunomodulatory agents on bovine humoral and cellular immune responses to parenteral inoculation with bovine rotavirus vaccines.sodium diethyldithiocarbamate (dtc), mycobacterium cell wall extract (mcwe, regressin), killed corynebacterium parvum (c. parvum, immunoregulin) and muramyldipeptide (mdp) were each combined with purified, live bovine rotavirus and inoculated into 3 month-old holstein-friesian calves in order to examine their ability to potentiate specific humoral and cellular immune responses. the vaccinated calves were boosted twice at 3 and 6 weeks after initial vaccine inoculation. the rotavirus was administ ...19882847397
normal immunosuppressive protein: in vitro inhibition of dna synthesis in t and b lymphocytes and lymphoid cell lines.the biologic activity of normal immunosuppressive protein (nip) isolated from human plasma was studied. nip was found to inhibit the proliferation of both t and b lympohcytes in vitro. it suppressed the dna synthesis of normal mouse lymphocytes responding to the mitogens phytohemagglutinin and lipopolysaccharide, as well as the [3h]thymidine and [3h]leucine uptake by t and b lymphoid cell lines of human and murine origin. the lymphoid specificity of nip was demonstrated by showing that dna and p ...197993092
specificity and t-lymphocyte dependence of the humoral immune response in the rat to purified ovine and porcine mucins. 197992759
detection by antihapten antibodies of liver-bound compounds related to azocarcinogens or their metabolites.the localization of known azocarcinogens and metabolites such as p-aminoazobenzene and n-methyl-p-aminoazobenzene bound to components of liver cells of rats fed single or multiple doses of 3'-methyl-p-dimethylaminoazobenzene has been determined with the use of antibodies raised against p'-azo-p-aminoazobenzene and p'-azo-n-monomethyl-p-aminoazobenzene in the indirect fluorescent antibody procedure. these 2 antisera reacted with liver cells of rats fed 3'-methyl-p-dimethylaminoazobenzene, p'-amin ...197990352
observations on the m-wave and the cnv in the squirrel monkey.a typical cnv paradigm, with food as reward, evokes in the squirrel monkey's post-arcuate and post-central cortices both m-waves in response to the cues and what appears to be a cnv in the interstimulus interval. both wave forms appear to be generated locally in cortex and to be more closely related temporally to the cues than to the animal's behavioral response. the cnv, like the m-wave, appears to reflect the animal's level of interest in obtaining the reward. it is smaller when the animal mis ...197985525
histamine concentration and ca2+ mobilization in arterial smooth muscle.smooth muscle contraction is dependent on ca2+ entry from the extracellular space or release from intracellular stores. the sensitivity of these ca2+ sources to agonist concentration was evaluated by measuring myoplasmic [ca2+] (as estimated by aequorin), myosin phosphorylation, and isometric stress in the swine carotid media. high histamine concentrations produced transient elevations in [ca2+] and phosphorylation with rapid generation of near maximal stress. lower histamine concentrations prod ...19892750885
decreased gastro-intestinal responses to salbutamol and serotonin in streptozotocin-induced diabetes: improving effect of insulin in vivo and in vitro.1. decreased gastro-intestinal responses to salbutamol (sal) and serotonin (5-ht) in experimental diabetes have been postulated. the present study was designed to investigate whether in vivo and in vitro insulin treatments improve the decreased gastro-intestinal responses. 2. in vivo insulin treatment (166.7 micrograms/kg/day i.p.) for 6 weeks is able to improve both decreased gastro-intestinal beta-adrenergic and serotonergic responses. 3. insulin incubation in bathing medium for 4-5 hr enhance ...19883063593
decreased gastro-intestinal responses to salbutamol and serotonin in streptozotocin-induced diabetes: improving effect of insulin in vivo and in vitro.1. decreased gastro-intestinal responses to salbutamol (sal) and serotonin (5-ht) in experimental diabetes have been postulated. the present study was designed to investigate whether in vivo and in vitro insulin treatments improve the decreased gastro-intestinal responses. 2. in vivo insulin treatment (166.7 micrograms/kg/day i.p.) for 6 weeks is able to improve both decreased gastro-intestinal beta-adrenergic and serotonergic responses. 3. insulin incubation in bathing medium for 4-5 hr enhance ...19883063593
effect of acute ethanol administration on human blood aldehyde dehydrogenase activity.the aldehyde dehydrogenase (ec activity in human blood was measured after acute oral administration of two different doses of ethanol to healthy subjects. no changes in aldehyde dehydrogenase activity were observed in either of the two dose groups, as compared to control subjects receiving no ethanol. likewise, incubation of human whole blood samples with 20 mm ethanol for 2 hr caused no change in aldehyde dehydrogenase activity. the results show that human blood aldehyde dehydrogenase ...19883067608
bone bonding behavior of mgo-cao-sio2-p2o5-caf2 glass (mother glass of a.w-glass-ceramics).in this study, it was found that a ca-p layer and a si layer were formed on the interface of the mother glass of apatite-wollastonite containing glass-ceramics (designated aw) and bone tissue. the dissolution of si, ca, and p from glass (mgo-cao-sio2-p2o5-caf2) is necessary to form a chemical film (a si layer and a ca-p layer). the three kinds of glasses used were 1) a mirror surface of the mother glass (mgo 4.6, cao 44.9, sio2 34.2, p2o5 16.3, caf 0.5 weight ratio) of aw (designated g-aw (mirro ...19892738079
improved sensitivity of an enzyme immunoassay ideia for detecting chlamydia tests on 375 genital tract specimens a commercially available enzyme immunoassay for chlamydia trachomatis (ideia; boots-celltech) was found to have sensitivity values of 62% for men and 74% for women, and a specificity of 97% for both groups, relative to the results obtained by a fluorescence assay (micro trak; syva). the positive predictive value and the negative predictive value of the immunoassay were 91% and 87%, respectively. collection of samples for ideia in transport medium in plasti ...19892668343
experimental evidence for enteropathogenicity in aeromonas veronii.eleven ornithine-positive strains of aeromonas (9 a. veronii and 2 provisionally classified as aeromonas species ornithine positive) were tested for ability to cause fluid accumulation in the rabbit ileal loop. all eight beta-hemolytic strains caused fluid accumulation. gel diffusion analysis revealed that the a. veronii beta-hemolysin was serologically related to the a. hydrophila beta-hemolysin, a known enterotoxic molecule. the biological activity of the a. veronii hemolysin was neutralized b ...19883143475
non-immune igg f(ab')2 binding to group c and g streptococci is mediated by structures on gamma chains.the present investigation was designed to determine whether the heavy or the light immunoglobulin chain is involved in the non-immune binding of igg f(ab')2 fragments to specific surface receptors on human group c and g streptococci. purified human polyclonal igg was mildly reduced with dithiothreitol and alkylated with iodoacetamide. light (l) and heavy (h) chains were separated. intact igg and purified l and h chains of polyclonal immunoglobulin g were tested in an inhibition assay for non-imm ...19853156400
delivered volume during high-frequency ventilation. 19892648741
[the body as hostage of the self. phenomenological aspects of anorexia nervosa].there are several basic structures upon which anorexia nervosa could develop: hostility between mother and daughter, feministic protest, abandonnism, the ascetic structure, the reluctance against the being-thrown-on-the-world, asw. the phenomenological analysis reveals, through these different structures, a common distress. the patient feels the others, and also, the existence, as a violence. yet, she feels help ass in her fight against this violence. so she takes her own body as hostage in orde ...19883207043
sexual maturation of turkish boys in ankara. 19883232223
comparative sequence analysis, in vitro expression and biosynthesis of mouse prp.a number of studies have indicated that an endogenous brain protein, prp, is associated with transmissible agents causing spongiform encephalopathies such as scrapie, kuru, and creutzfeldt-jakob disease. it has been proposed that prp derived from scrapie brain is the scrapie agent itself. to test directly whether the prp mrna in scrapie brain tissue can encode the scrapie agent, we expressed prp cdna cloned from scrapie-infected mouse brain in vitro. the expressed prp did not transmit scrapie to ...19892574873
glutamate degradation in the ischemic dog heart: contribution to anaerobic energy production.the present study investigated the conversion of amino acids to succinate and the contribution of this pathway to anaerobic energy production during regional ischemia in the dog heart in situ. the relation between regional myocardial blood flow, estimated by the tracer microsphere technique, and myocardial contents of metabolites (glutamate, alanine, succinate, lactate) as well as their local arterio-venous differences (a-v) were determined. during 30 min of coronary artery occlusion, myocardial ...19892565982
[medico-genetic studies of human provisional organs]. 19873318529
whole-body autoradiography of [3h]dihydrostreptomycin in guinea pigs and rats: the labelling of the inner ear in relation to other tissues.[3h]dihydrostreptomycin was given intramuscularly to young pigmented guinea pigs and rats. whole-body autoradiography, combined with densitometric measurement of the blackening of the autoradiograms, and liquid scintillation counting were used to determine the levels of radioactivity in the inner ear in relation to blood and other tissues. it was found that there was an accumulation of radioactivity in the perilymph of both the cochlear and vestibular parts of the labyrinth. the labelling of the ...19862422870
evaluation of prostaglandins as mediators of tubuloglomerular feedback.orthograde and retrograde microperfusion experiments were conducted in sprague-dawley rats to evaluate the participation of vasoconstrictive eicosanoids as mediators of tubuloglomerular feedback (tgf) signals. retrograde perfusion with 160 microm arachidonic acid (aa) added to a hypotonic solution enhanced the stop-flow pressure (sfp) feedback responses compared with those obtained with the control hypotonic solution (delta sfp, 1.6 +/- 0.4 vs. 10.1 +/- 0.7 mmhg with aa). blockade of thromboxane ...19883364575
toxicology of selected pesticides, drugs, and chemicals. organophosphorus and carbamate insecticides.organophosphorus and carbamate insecticides produce acute toxicosis via a common mechanism--the inhibition of acetylcholinesterase--and, therefore, these categories of insecticides are considered together. this article discusses the mechanisms, toxicity, clinical signs, lesions, and diagnosis of, as well as treatment for, toxicoses produced by these compounds.19902180181
gas-liquid chromatographic analysis of cellular fatty acid methyl esters in aeromonas species.the cellular fatty acids of 39 strains belonging to the genus aeromonas (aeromonas hydrophila, aeromonas caviae, aeromonas sobria, aeromonas media, aeromonas schubertii, aeromonas veronii) were determined by high resolution gas-liquid chromatography. the fatty acid profiles were characterized by major amounts (60% or more) of one saturated (hexadecanoic acid = 16:0) and two unsaturated (hexadecenoic acid = 16:1 and octadecenoic acid = 18:1) acids. while the majority of the strains of the six spe ...19911930556
retinal neurite growth on astrocytes is not modified by extracellular matrix, anti-l1 antibody, or oligodendrocytes.two factors that may influence the course of axonal regeneration in the central nervous system (cns) are extracellular matrix (ecm) and cell surface molecules that may enhance or inhibit neurite outgrowth. whereas cultured astrocytes have been reported to be a good substratum for neurite outgrowth, there is recent evidence that cultured oligodendrocytes are inhibitory. to test the influences of 1) ecm components, 2) the l1 adhesion molecule, and 3) the inhibitory potential of mature oligodendroc ...19911828788
thermally-induced delayed fluorescence of photosystem i and ii chlorophyll in thermophilic cyanobacterium synechococcus elongatus.stationary delayed fluorescence (df) of chlorophyll in isolated membrane preparations from thermophilic cyanobacterium synechococcus elongatus was investigated as a function of temperature. two peaks at different temperatures were observed. the low-temperature peak (54-60 degrees c) coincided with the main maximum of the thermally-induced delayed fluorescence of chlorophyll in intact cells and psii-particles with active oxygen-evolving system. the high-temperature peak (78 degrees c) coincided w ...19921426973
halogenated isoniazid derivatives as possible antitubercular and antineoplastic agents. note 1.halogenated arylhydrazones, 2-aryl-4-thiazolidinones and their 1,1-dioxides containing isoniazid (ini) moieties were prepared and explored for antimicrobial (against bacteria, candida and mycobacteria) and antitumor activities. none of the tested compounds showed any marked antimicrobial effect. furthermore, among the compounds submitted to nci in vitro primary antitumor screening, those bearing 3-fluoro or -chlorophenyl substituents were found to possess selective inhibitory effects on the grow ...19921388607
aeromonas veronii, a new ornithine decarboxylase-positive species that may cause 1983, the vernacular name enteric group 77 was coined for a group of strains that had been referred to our laboratory as "possible vibrio cholerae except for gas production." by dna-dna hybridization (hydroxyapatite, 32p), 8 of 10 strains of enteric group 77 were very highly related to the labeled strain 1169-83 (74 to 100% at 60 degrees c and 75 to 100% at 75 degrees c; percent divergence, 0.0 to 2.5). type strains of six other aeromonas species were 45 to 66% related (60 degrees c) to strai ...19873584425
[h2-receptor antagonist-refractory ulcer--its pathophysiology and role of proton-pump inhibitors].among h2-receptor antagonist (h2ra)-refractory ulcers, non-responders that did not heal after 5 months therapy had high intraluminal ph in the basal condition and high sensitivity to inhibition of acid secretion by h2ra but possessed gastric mucosa to generate less prostaglandins. combination therapy of pge1-analogue with h2ra healed these ulcers by 60%. proton-pump inhibitor (ppi) exerted a complete inhibition of acid secretion in these patients and the rate of healing was 88%. helicobacter pyl ...19921347325
chromosome banding patterns of 49 cases of chronic myelocytic leukemia. 1977266114
5-hydroxytryptamine receptor activity of the dopamine receptor agonist fenoldopam in canine tracheal smooth muscle.fenoldopam is a new vasodilator undergoing clinical trials for the treatment of hypertensive emergencies. its pharmacologic effects result from activation of vascular dopamine-1 receptors. in canine tracheal smooth muscle strips, fenoldopam caused a concentration- and calcium-dependent increase in tension, which was not antagonized by atropine, indomethacin or the dopamine-1 receptor antagonist, sch 23390. the ec50 (1.89 x 10(-6) m) exceeded that of serotonin or acetylcholine (8.38 x 10(-8) and ...19921346635
[changes in the composition of intracellular lipids and hydrocarbons in candida tropicalis in the absence of growth].the fatty acid composition of lipids and the composition of hydrocarbons were studied in candida tropicalis cultivated on a medium with propionic acid and incubated in the conditions of starvation and on a medium containing glucose-1-6-14c but no nitrogen. intracellular fatty acids with an odd number of carbon atoms were found to be easily metabolized by yeast cells. the content and composition of intracellular hydrocarbons were very labile and underwent intensive transformation caused by change ...19751214607
identification and characterization of an 8-kd peptide stimulating late erythropoiesis.chronic renal failure is often associated with severe anemia, subnormal levels of erythropoietin (epo), and the presence of erythropoietic inhibitors. we studied an anephric patient with polycystic kidney disease maintaining a hemoglobin level of 13-14 g. biochemical, endocrine, and hematological parameters were all within normal limits. epo levels examined on two occasions during the clinical investigation were found to be low to subnormal. plasma of this patient supported human bone marrow ery ...19892753086
in vitro evidence for an inhibitor of lipogenesis in serum from overfed obese rats.involvement of a humoral agent in regulation of energy balance has been demonstrated by parabiosis experiments. overfed obese rats produce a blood-borne factor that inhibits adipose fatty acid synthesis in their partners, resulting in loss of body fat without significant inhibition of food intake. an in vitro bioassay was developed to test small serum samples for antilipogenic activity. epididymal adipocytes from ad libitum-fed rats were preincubated for 12 h with 2% serum. basal adipocyte fatty ...19892764157
death due to disruption of sternal fixation wire--a rare complication after open heart surgery through sternotomy: report of a case.following sternotomy for open heart surgery, a 57-year-old man had the sternum closed in the usual manner with six no.22 gauge stainless steel wire sutures. twenty-four days later, sternum disruption occurred with only one wire suture in place, 3 wire sutures cutting through the bone, and 2 wire sutures fractured. a lacerating injury over the anterior surface of the right ventricle was rendered by these 2 fractured wires followed by fatal outcome. to our knowledge, death induced by fractured wir ...19892794943
[the effect of progesterone, testosterone and estradiol on steroid hormone release by the ovarian follicles of cows in vitro]. 19892806660
[effect of titanium phosphate, zirconium oxide and a mixture of ammonium phosphotungstenate with zirconium phosphate on the elimination of radioactive cesium from the bodies of rats]. 19853835674
serotonergic regulation of the brain and gut beta-endorphin and dynorphin content in the rat.a specific radioimmunoassay was used to measure immunoreactive dynorphin (ir-dyn) and beta-endorphin (ir-be) in the brain, pituitary and gut, following a pharmacological manipulation of the serotonin system. administration of the serotonin receptor agonist m-chlorophenylpiperazine (m-cpp, 2.5-5 mg/kg ip) or the serotonin releasing agent fenfluramine (20-40 mg/kg ip) induced a significant increase in the hypothalamic ir-be content and a decrease in its anterior pituitary level. these effects were ...19852870487
anatomical defects and variations due to aneuploidy. 19872953395
[effect of the culture medium composition on bacitracin synthesis and sporulation in bacillus lichenformis 28 ka].the effect of the culture medium composition on the bacitracin synthesis and sporulation in bacillus licheniformis 28 ka was being studied. during the producer's growth on a medium with tripton a biphasic pattern of the process was observed, i.e. a rapid growth of the bacterial biomass was accompanied by the production of insignificant amounts of the antibiotic. the maximum efficiency of the antibiotic synthesis coincides with the end of the exponential growth phase and the onset of sporificatio ...19863952068
interferon-gamma-induced degradation of tryptophan by human cells in vitro.several human cells were investigated for their ability to degrade tryptophan and to synthesize neopterin upon induction by interferon-gamma (500 units/ml for 48 h). concentrations of tryptophan, kynurenine, 3-hydroxykynurenine, anthranilic acid, 3-hydroxyanthranilic acid, 7,8-dihydroneopterin and neopterin were assessed in the culture supernatants by hplc. fibroblasts, a-22 arachnoidea, hk-2351 scalp, t-2346 meningeom and hela cervical carcinoma cells but not hl-60 promyelocytic leukaemia cells ...19873122784
thyrotropic and peripheral activities of thyrotrophin and thyrotrophin-releasing hormone in the chick embryo and adult chicken.the influence of an intravenous injection of thyrotrophin-releasing hormone (trh) and bovine thyrotrophin (tsh) on circulating levels of thyroid hormones and the liver 5'-monodeiodination (5'-d) activity is studied in the chick embryo and the adult chicken. in the 18-day-old chick embryo, an injection of 1 microgram trh and 0.01 i.u. tsh increase plasma concentrations of triiodothyronine (t3) and of thyroxine (t4). trh, however, preferentially raises plasma levels of t3, resulting in an increase ...19883133299
mutational specificity and the influence of excision repair: insights into the mechanisms of error-avoidance and error-fixation.the excision repair process controlled by the uvrabc gene in escherichia coli is the major pathway for the repair of a diverse series of dna damages. to achieve a better understanding of the mechanics of this repair pathway and its impact upon mutagenesis, we have applied a recently developed technology by which the nature of mutation is determined at the dna sequence level. a comparison of the classes and distribution of mutation in excision-repair-proficient and excision-repair-deficient strai ...19892698833
the purification of portuguese man-of-war nematocyst toxins by gel diffusion. 19734150989
[influence of temperature on the development of experimental infection of the newborn mouse by coxsackie virus]. 19664222899
occupational pigmented contact dermatitis from azo-dyes.a 51-year-old man had been working in a dye factory for 25 years and had noticed itching and pigmentation on the extremities for the past 5 years. patch testing showed positive reactions to sudan i and vacanceine red, among the azo dyes which he had been handling. after he changed his work, he became free from erythematous lesions with itching. the pigmentation almost disappeared 10 months later.19853156714
selective glycogen depletion in skeletal muscle fibres of man following sustained contractions.1. six healthy males performed sustained contractions with different tensions related to their maximal voluntary contraction (mvc). the isometric exercise consisted of efforts to extend the knee when flexed at an angle of 90 degrees .2. biopsy samples were taken from the lateral portion of m. quadriceps femoris before and after different periods (6-45 min) during a series of sustained contractions. total glycogen content was determined on each muscle sample. in order to evaluate whether the glyc ...19744278538
the response of the pituitary-adrenal system to a stressful stimulus: the effect of conditioning and pentobarbitone treatment. 19654284301
[a lipoma of the cerebellopontile angle].the case of a 38-year old man with a lipoma of the left cerebellopontine angle is presented. while the clinical signs of that tumour are unspecific, an exact diagnosis is possible by ct and mri. as in other reported cases only an incomplete removal of the tumours was made because of the adherence of the lipoma to the nerves of the cpa.19883261397
[a lipoma of the cerebellopontile angle].the case of a 38-year old man with a lipoma of the left cerebellopontine angle is presented. while the clinical signs of that tumour are unspecific, an exact diagnosis is possible by ct and mri. as in other reported cases only an incomplete removal of the tumours was made because of the adherence of the lipoma to the nerves of the cpa.19883261397
glucagon stimulation of adenyl cyclase activity of cardiac muscle. 19684302984
glucagon stimulation of adenyl cyclase activity of cardiac muscle. 19684302984
neutralization of the anticomplementary activity of an amorphous silica by some proteins. 19674305245
sarcoma blockade in vivo. rabies-rous system in chickens. 19704320567
coronary vascular actions of stroma-free hemoglobin preparations.the left anterior descending coronary artery was cannulated in anesthetized, open-chest dogs for use as a coronary test bed to assess the coronary vascular actions of oxygenated, unmodified and pyridoxylated, partially cross-linked (polymerized), stroma-free hemoglobin solutions (sfhs). the actions of these sfhss were assessed in this test bed, by comparison with perfusion with whole blood. unmodified sfhs caused significant vasoconstriction, whereas pyridoxylated, partially polymerized sfhs did ...19883355384
avian leukosis-sarcoma virus antibodies in wildfowl, domestic chickens and man in kenya. 19734358887
a procedure of vestibular decompensation for clinical diagnosis. i. animal experiments.vestibular lesions are often so effectively compensated that their diagnosis may be difficult. certain chemicals can decompensate the central vestibular system and thus uncover symptoms present before compensation. this study attempts to devise a procedure which can utilize such decompensation for the benefit of clinical diagnosis. unilateral labyrinthectomies were performed in rats. pathological movements and body positions due to the vestibular loss were compensated within about one week. syst ...19863485723
[a study of fructose metabolism in the human liver]. 19714399035
trimethoprim used for selective decontamination of the digestive tract in rats: possible route of excretion.selective elimination of enterobacteriaceae species from the digestive tract of rats has been accomplished by oral treatment with trimethoprim (235 mg/kg body weight/day) within 6 days. in the present study it was investigated whether this elimination was mainly due to antimicrobial activity of trimethoprim excreted with the gastrointestinal mucus or mainly by non-absorbed trimethoprim in the lumen contents. by means of whole gut irrigation (wgi) the lumen contents were washed out, followed by m ...19873497436
effect of oral chenodeoxycholic acid on bile acid kinetics and biliary lipid composition in women with cholelithiasis.bile acid kinetics and biliary lipid composition were characterized in six women with gallstones before and after 6 mo of oral therapy with chenodeoxycholic acid, an agent that induces dissolution of cholesterol gallstones in man. over a dosage range of 1-4 g/day, absorption varied from 0.8 to 2.3 g/day. the chenodeoxycholic acid pool expanded two-to sixfold, and bile became composed predominantly (> 90%) of chenodeoxycholic acid conjugated chiefly with glycine. cholic acid and deoxycholic acid ...19734583981
hypertension as a localizing sign of mass lesions of brainstem. 19724635025
a practicable variant of the ion exchange method for the radiometric estimation of ornithine decarboxylase activity.a known ornithine decarboxylase assay working with ion exchange separation of [3h]ornithine and [3h]putrescine has been revised. the assay can be performed in disposable 1.5 ml vessels with a total of four pipetting steps. the separation of enzyme substrate and product, respectively, requires 3 h per 50 samples. the detection limit is about 50 pmoles [3h]putrescine formed.19873545892
retinol glycolipids. 19734684696
[comparative studies on cerebral vessel pathology in animals and man]. 19734725275
fetal-type creatine kinase in rat fast and slow muscles during denervation and reinnervation.creatine kinase (ck) has three forms of isozymes; ck-bb, ck-mb, and ck-mm. in adult rats they show a specific tissue distribution: the bb form in the brain, the mb form in the heart, and the mm form in skeletal muscle. in embryonic skeletal muscles only the bb and mb forms are found. adult slow-twitch muscles contain more fetal type creatine kinase (ck-b) than do fast-twitch muscles. in the present experiment the effect of denervation and reinnervation on the ck-b concentration was investigated ...19873556205
reversible damage of rat upper respiratory tract caused by cigarette smoke. 19744809936
fetal-type creatine kinase in rat fast and slow muscles during denervation and reinnervation.creatine kinase (ck) has three forms of isozymes; ck-bb, ck-mb, and ck-mm. in adult rats they show a specific tissue distribution: the bb form in the brain, the mb form in the heart, and the mm form in skeletal muscle. in embryonic skeletal muscles only the bb and mb forms are found. adult slow-twitch muscles contain more fetal type creatine kinase (ck-b) than do fast-twitch muscles. in the present experiment the effect of denervation and reinnervation on the ck-b concentration was investigated ...19873556205
[effects of inosine on the transmembrane potential and contractility of the hypoxic cardiac muscle]. 19873686054
inhibition of liver glutathione s-transferase activity in rats by hypolipidemic drugs related or unrelated to clofibrate.the effects of in vivo administration of six hypolipidemic drugs on rat liver glutathione s-transferase activity were compared. this activity was measured with sulfobromophthalein (bsp), 1,2-dichloro-4-nitrobenzene (dcnb) or 1-chloro-2,4-dinitrobenzene (cdnb) as substrate. except for the nicotinic acid derivative ethanolamine oxiniacate, all the compounds tested significantly reduced it, whether or not they were related to clofibrate. the hepatic glutathione concentration either remained unchang ...19863707598
disproportional changes in the rates of biliary bile acid, phospholipid and cholesterol secretion in the shr/n-corpulent rat.lean and obese male rats of the shr/n-cp strain were fed a plain chow diet ad libitum and studied at 11-13 weeks of age. compared to their lean littermates the obese animals manifested 2-4-fold higher plasma levels of cholesterol, triacylglycerol and glucose. in the obese rats, the mass of the liver was increased more than 70% and there was a significant increase in the total hepatic content of free cholesterol (42%), cholesteryl ester (2.9-fold) and triacylglycerol (7.4-fold). despite the diffe ...19863768384
effect of dilauroylphosphatidylcholine on the acrosome reaction and subsequent penetration of bull spermatozoa into zona-free hamster eggs.incubation of bull sperm with liposomes made with phosphatidylcholine (pc) containing fatty acyl chains of either 10 (pc10) or 12 (pc12) carbons resulted in greater than 90% of the sperm exhibiting an acrosome reaction (ar) within 15 min. liposomes of pc10 rapidly destroyed sperm motility while pc12 acrosome-reacted sperm remained motile for several h. liposomes of pc with greater than or equal to 14-carbon fatty acyl chains had no effect on the ar or motility of sperm. the ar was not induced by ...19863768462
demonstration of human reagin in monkey tissues. 3. in vitro passive sensitization of monkey ileum with sera of atopic patients. physiologic and enhancing experiments. 19674961409
microsomal binding sites for antioestrogens in rat liver. properties and detergent solubilization.the properties of an antioestrogen binding site (aebs), which has high affinity and specificity for nonsteroidal antioestrogens and structurally related compounds, have been studied in rat liver microsomes. when subcellular organelles were separated on percoll density gradients the distribution of the aebs paralleled that of nadph-cytochrome c reductase, indicating that the aebs is associated with the endoplasmic reticulum. saturation analysis showed that [3h]tamoxifen was bound to a single clas ...19863790095
a simple and rapid method for quantitative determination of protein in urine. 19725061737
electron microscopic examination of factors influencing the expression of filamentous surface structures on clinical and environmental isolates of aeromonas veronii biotype sobria.strains of aeromonas veronii biotype sobria isolated from clinical and environmental sources were examined for their expression of surface structures under a variety of culture conditions. when grown on solid media at 37 degrees c, more than 95% of bacteria from the majority of strains isolated from human diarrheal feces and chicken carcasses were non-piliated or expressed only a few pili of long, flexible morphology per cell. strains isolated from water or other foods were much more likely to e ...19957565173
a specific puld homolog is required for the secretion of paracrystalline surface array subunits in aeromonas hydrophila.aeromonas hydrophila is an important pathogen of fish, and its high-virulence strains display a two-dimensional paracrystalline layer (s-layer) on their outermost surfaces. the nucleotide sequence of a 4.1-kb region located 700 bp upstream of the a. hydrophila tf7 s-layer protein gene (ahsa) has been determined. a sequence analysis of the region revealed the presence of three complete open reading frames ending in a gene encoding a 79.8-kda polypeptide that shows high homology to the puld family ...19957608063
case of aeromonas veronii (dna group 10) bacteremia.we describe the first case report of bacteremia due to aeromonas veronii biotype veronii. the infection occurred in a 77-year-old man suffering from multiple underlying conditions which included cancer of the sigmoid colon. because of the unusual biochemical phenotype of this group (ornithine decarboxylase positive), it was originally identified as vibrio cholerae.19947883912
aeromonas species in septicemia: laboratory characteristics and clinical observations.we retrospectively analyzed clinical and epidemiological data on and laboratory characteristics of 53 cases of aeromonas septicemia. only four aeromonas genomospecies (species defined by dna relatedness) were associated with the 53 cases, with aeromonas hydrophila (sensu stricto) predominating (47%). nearly 60% of all aeromonas isolates from blood fell into one of four somatic groups: serogroups o:11, o:16, o:18, and o:34. unlike aeromonas-associated gastroenteritis, septicemia did not peak in f ...19947948561
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