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structural and pathogenic properties of aeromonas schubertii.we investigated the phenotypic, structural, and pathogenic properties of 11 aeromonas schubertii strains recovered from extraintestinal sites. most a. schubertii strains were autoagglutination positive, possessed a high surface charge but low hydrophobicity, and fell into one or two biogroups on the basis of carbon substrate utilization patterns. fatty acid methyl ester analysis of a. schubertii revealed this species to contain a relatively high percentage of branched fatty acids (i-13:0, i-15:0 ...19921563798
gas-liquid chromatographic analysis of cellular fatty acid methyl esters in aeromonas species.the cellular fatty acids of 39 strains belonging to the genus aeromonas (aeromonas hydrophila, aeromonas caviae, aeromonas sobria, aeromonas media, aeromonas schubertii, aeromonas veronii) were determined by high resolution gas-liquid chromatography. the fatty acid profiles were characterized by major amounts (60% or more) of one saturated (hexadecanoic acid = 16:0) and two unsaturated (hexadecenoic acid = 16:1 and octadecenoic acid = 18:1) acids. while the majority of the strains of the six spe ...19911930556
characterization of aeromonas schubertii strains recently isolated from traumatic wound infections.recent studies have resulted in the proposal of a new species, aeromonas schubertii (mannitol, sucrose, and indole negative), formerly termed enteric group 501, on the basis of the study of seven strains isolated from the southeastern and southwestern united states and puerto rico. we have isolated two phenotypically similar a. schubertii strains from infected human wounds sustained in the chesapeake bay area. their identification was confirmed by dna-dna hybridization to the centers for disease ...19892768470
aeromonas schubertii, a new mannitol-negative species found in human clinical specimens.in 1983 the vernacular name enteric group 501 was coined for a group of strains that had been referred to our laboratory as "possible vibrio damsela that does not require nacl for growth." by dna-dna hybridization (hydroxyapatite method, 32p, 60 and 75 degrees c), six strains of enteric group 501 were closely related to the labeled strain 2446-81 (70 to 95% at 60 degrees c and 71 to 93% at 75 degrees c; 0 to 1% divergence). type strains of all aeromonas species and reference strains of six other ...19883139706
dna relatedness among aeromonas allosaccharophila strains and dna hybridization groups of the genus aeromonas.the genomic relatedness among three aeromonas allosaccharophila strains, including the type strain, and other aeromonas type and reference strains that were assigned to dna hybridization groups was estimated by dna-dna hybridization (competition procedure using a membrane method). all a. allosaccharophila strains were highly related (70 to 100%) to strains 289t (= cect 4199t) and atcc 35942. type strains of other validated aeromonas species, reference strains of dna groups 8 and 11, and the aero ...19957727276
identification of aeromonas schubertii and aeromonas jandaei by using a polymerase chain reaction-probe test.two oligonucleotide primers were used in a polymerase chain reaction-protocol to amplify a region (approx. 850 bp) of the 16s rrna gene of aeromonas schubertii and aeromonas jandaei. hybridization of the polymerase chain reaction products to specific internal probes provided a highly specific method for the identification of these two species.19938486241
misidentification of unusual aeromonas species as members of the genus vibrio: a continuing problem.two unusual cases of aeromonas infection are described, one associated with bacteremia (aeromonas schubertii) and another in which the organism was recovered from an infected gall bladder (aeromonas veronii biotype veronii). these strains were initially identified as vibrio damsela and vibrio cholerae by the vitek and api 20e systems, respectively. use of appropriate screening tests and familiarity with the newer aeromonas species could prevent initial misidentifications and potential public hea ...19989542946
phylogenetic positions of aeromonas encheleia, aeromonas popoffii, aeromonas dna hybridization group 11 and aeromonas group 501.the 16s rdna sequences of the recently described aeromonas encheleia and aeromonas popoffii, were determined and compared with data from all known aeromonas sp. diagnostic 16s rdna regions were also sequenced for some strains previously considered as an extension of a. encheleia and a strain of aeromonas group 501 (formerly enteric group 501). results indicated that a. encheleia and a. popoffii are phylogenetically separated species as originally described. a conclusion about hg11 taxonomic stat ...199910555319
interaction of cefotetan and the metallo-beta-lactamases produced in aeromonas spp. and in vitro activity.aeromonas spp. are increasingly being recognized as human pathogens. the presence of metallo-beta-lactamases in these organisms represents a potential problem in antimicrobial therapy. mechanism-based inactivators of beta-lactamases are used to overcome the resistance of clinical pathogens to beta-lactam antibiotics, but no clinical useful inhibitors of the metallo-beta-lactamases are presently known. studying the interaction between cefotetan and aeromonas spp. producing metallo-beta-lactamase ...200010765032
mesophilic aeromonads in wild and aquacultured freshwater fish.numbers and species of motile aeromonas were determined in freshly caught freshwater fish, in the surrounding environment, and also during iced chilled storage of fish specimens. although no significant differences were observed in water samples, initial levels for skin, gill, and intestines were significantly lower in farmed rainbow trout (oncorhynchus mykiss) than in wild brown trout (salmo trutta) and pike (esox lucius). during storage of wild specimens, naturally occurring aeromonads grew fa ...200111348001
the occurrence of enteric pathogens and aeromonas species in organic vegetables.a range of commercially available organic vegetables (n = 86) was examined for the presence of salmonella, campylobacter, escherichia coli, e. coli o 157. listeria and aeromonas spp., to provide information on the occurrence of such organisms in organic vegetables in northern ireland. the study was not designed to quantify such organisms or to compare occurrence with conventionally farmed vegetables. standard enrichment techniques were used to isolate and identify enteric pathogens and aeromonas ...200111759753
aeromonas simiae sp. nov., isolated from monkey faeces.two aeromonas strains, ibs s6874(t) and ibs s6652, were isolated from the faeces of two healthy monkeys (macaca fascicularis) from mauritius that were kept in quarantine in the centre for primatology, strasbourg, france. phylogenetic analysis based on 16s rrna gene sequences showed that the two isolates formed an unknown genetic lineage within the genus aeromonas. the two isolates had nearly identical sequences (0.1 % nucleotide substitution) that were related closely to those of recognized aero ...200415023964
[discordancies between classical and api 20e microtest biochemical identification of vibrio and aeromonas strains].the present study reveals some discordancies of diagnosis considering vibrionaceae strains when tested by comparison with api 20e (biomerieux) system and classical biochemical reactions, aspects already mentioned in the literature by other authors. if any misidentification has not an important impact (e.g. identification of aeromonas schubertii as a. sobria), others as misidentification of aeromonas as vibrio strains or the failure to recognize vibrio strains raise major epidemiological problems ...200315341329
rabbit meat as a source of bacterial foodborne pathogens.even though worldwide production of rabbit meat is >1,000,000 tons, little information is available for rabbit meat microbiology. this study provides data on the prevalence of salmonella, escherichia coli o157:h7, yersinia enterocolitica, listeria spp., motile aeromonas spp., and staphylococcus aureus on rabbit meat. a total of 24 rabbit carcasses from two abattoirs and 27 rabbit meat packages from supermarket displays were examined. in addition to culturing methods, associated virulence genes w ...200616715811
updated phylogeny of the genus aeromonas.recent phylogenetic studies of the genus aeromonas based on gyrb and rpod gene sequences have improved the phylogeny based on 16s rrna gene sequences first published in 1992, particularly in the ability to split closely related species. these studies did not include the recently described species aeromonas simiae and aeromonas molluscorum and only a single strain of aeromonas culicicola was available for analysis at that time. in the present work, these aeromonas species and newly isolated strai ...200617012583
[characterization of aeromonas spp isolates from newborns hospitalized].aeromonas spp is recognized as pathogenic to humans after consumption of contaminated water and food. in the present investigation, 2,323 rectal swab samples from newborns hospitalized in rio de janeiro were evaluated with a view to isolating aeromonas. the samples were collected and sent to the national reference laboratory for cholera and other bacterial intestinal infections, at the oswaldo cruz institute of the oswaldo cruz foundation. the swabs were subjected to enrichment in alkaline pepto ...200818545840
isolation and identification of two novel sds-resistant secreted chitinases from aeromonas schubertii.two sds-resistant endochitinases, designated as aschi53 and aschi61, were isolated from aeromonas schubertii in a soil sample from southern taiwan. maldi-tof mass measurement indicates the molecular weights of 53,527 for aschi53 and 61,202 for aschi61. n-terminal and internal amino acid sequences were obtained, and blast analysis of the sequences and ms/ms peptide sequencing showed that they were novel proteins. degradation of chitin by these two endochitinases gave rise to hexameric chitin olig ...200919197977
a fatal case of necrotizing aeromonas schubertii fasciitis after penetrating injury. 201121247722
effects of bengal gram on cholesterol induced atherosclerosis in rabbits. 19725017797
concurrent schedule control of human visual target fixations.operant conditioning techniques were applied to the study of how target fixations are controlled by the probability of signal occurrence. in a standard vigilance setting, gaze at three illuminable volt meters was monitored by a mackworth television eye camera with automatic recording capability. gaze at a given meter produced illumination of the meter, and signals (deflections of the needle on the meter) were scheduled as intermittent consequences of this response. target fixations were thus pla ...19734759060
role of methionine in the initiation of the biosynthesis of bovine proinsulin. 19734717064
effect of erythropoietin preparations on cyclic amp and cyclic gmp levels in rat fetal liver cell cultures. 1977194988
first pediatric case of chromobacterium haemolyticum causing proctocolitis.bloody diarrhea in children is usually due to either infectious or inflammatory etiology, but infection is far more common than inflammatory bowel disease in children worldwide. if, however, the patient has unfavorable response to antibiotics and a definite infectious agent has yet to be identified; colonoscopy should be performed. the current patient presented with acute onset of mucous bloody diarrhea. stool culture was initially identified as vibrio mimicus and later identified as aeromonas s ...201425252051
first record of the rare species aeromonas schubertii from mussels: phenotypic and genetic reevaluation of the species and a review of the literature.in a study where the prevalence of aeromonas in shellfish was analysed, three isolates of aeromonas schubertii were identified, representing this the first report of this species from mussels. this species was originally described in 1988 from strains isolated from extra-intestinal human infections and since then has been cited in only 18 occasions. for many years, a. schubertii was the only mannitol-negative species of the genus. however, three additional mannitol-negative species (aeromonas si ...201626825089
occurrence, molecular characterization, and antimicrobial susceptibility of aeromonas spp. in marine species of shrimps cultured at inland low salinity ponds.we aimed to document the risk of aeromonas spp. in marine shrimp species cultured in inland low salinity ponds in thailand. in 14 of 18 shrimp samples retrieved from inland grow-up ponds, aeromonas spp. were detected at ranges from 4667 to 1,500,000 cfu/g body weight. the phylogenetic tree constructed with the gyrb and cpn60 concatenated sequences indicated that the 87 isolates consisted of aeromonas veronii (70%), aeromonas aquariorum (18%), aeromonas caviae (7%), aeromonas jandaei (2%), and ae ...201525583334
alternative first-line therapies in geriatric hypertension.between 45 and 60% of elderly persons have mild to moderate hypertension. in those with combined systolic/diastolic hypertension, blood pressure reduction significantly reduces morbidity and mortality. secondary causes of hypertension, particularly renovascular, should be considered in elderly patients with a recent history of hypertension. because of their proven efficacy and low cost, low-dose thiazide diuretics remain an important first-line therapy. angiotensin-converting enzyme (ace) inhibi ...20092647588
agoraphobia and parental bereavement.the prevalence of parental bereavement was determined in 50 married female outpatients with a dsm-iii diagnosis of agoraphobia and in a control group of married female outpatients diagnosed as having non-psychotic psychiatric disorders other than agoraphobia. the two groups were matched for age and overall severity of psychiatric symptoms. compared with the general population, the patient control group reported a statistically significant excess of parental, but not maternal, bereavement. the ag ...20123435374
[epidemiological survey of oral diseases in a greek population. i. cephalonia island]. 1998106348
[differential diagnosis of the complications of acute cranio-cerebral injuries]. 20164722262
microbial diversity and potential pathogens in ornamental fish aquarium water.ornamental fishes are among the most popular and fastest growing categories of pets in the united states (u.s.). the global scope and scale of the ornamental fish trade and growing popularity of pet fish in the u.s. are strong indicators of the myriad economic and social benefits the pet industry provides. relatively little is known about the microbial communities associated with these ornamental fishes or the aquarium water in which they are transported and housed. using conventional molecular ...201222970112
optimization of recombinant hexaoligochitin-producing chitinase production with response surface methodology.hexaoligochitin produced by chitinase, aschi61, from aeromonas schubertii was recently expressed. in this work, the optimal conditions for the mass production of aschi61 were investigated. the efficiency of recombinant protein expression in escherichia coli was determined by various parameters, including the ph of the culture medium, induction temperature, shaking speed, inducer concentration, and induction period. the optimization experiments could be simplified through a statistical design of ...201324099937
first case of aeromonas schubertii infection in the freshwater cultured snakehead fish, ophiocephalus argus (cantor), in china.an epizootic in snakehead fish, ophiocephalus argus, in earthen ponds in xianning, hubei province, central china, from june to august 2009 was found to be caused by aeromonas schubertii. the cumulative mortality within 40 days was 45%, and the diseased fish were 18 months old and 35-45 cm in length. multiple, ivory-white, firm nodules, 0.5-1 mm in diameter, were scattered throughout the kidney. blood clots, 3-5 mm in diameter, were found in the liver. this is a disease frequently found in cultur ...201222417292
complete genome sequence of the type strain of aeromonas schubertii, atcc 43700.we sequenced the complete genome of the type strain of aeromonas schubertii, atcc 43700. the full genome sequence of a. schubertii atcc 43700 is 4,356,858 bp, which encodes 3,842 proteins and contains 110 predicted rna genes.201626893413
isolation and characterization of aeromonas schubertii from diseased snakehead, channa maculata (lacepède).pure bacterial cultures were isolated from diseased snakeheads, channa maculata (lacepède), suffering high mortality in a farm in zhongshan, southern china. three isolates, namely zs20100725, zs20100725-1 and zs20100725-2, were identified as aeromonas schubertii. all the isolates showed high 16s rrna sequence similarities with a. schubertii. the isolates exhibited strong virulence to snakeheads in experimental challenges with ld(50) ranging between 1.4 × 10(4) and 6.4 × 10(6) cfu g(-1). two of t ...201222524539
complete genome sequence of the highly virulent aeromonas schubertii strain wl1483, isolated from diseased snakehead fish (channa argus) in china.we sequenced the complete genome of the highly virulent aeromonas schubertii strain wl1483, which was isolated from diseased snakehead fish (channa argus) in china. the full genome sequence of a. schubertii wl1483 is 4,400,034 bp, which encodes 4,376 proteins and contains 195 predicted rna genes.201626798095
draft genome sequence of the aeromonas diversa type strain.we present here the first genome sequence of the aeromonas diversa type strain (cect 4254(t)). this strain was isolated from the leg wound of a patient in new orleans (louisiana) and was originally described as enteric group 501 and distinguished from a. schubertii by dna-dna hybridization and phenotypical characterization.201323792745
the characteristics of chitinase expression in aeromonas schubertii.in this study, chitinase activity in an incubation broth of aeromonas schubertii was measured using colloidal chitin azure as the substrate. more specifically, the induction of chitinases due to amendment with various carbon sources was examined. the highest chitinase activity was found following amendment with 0.5-1.0 % chitin powder, whereas the activity increased negligibly due to amendment with other carbon sources, such as glucose, glcnac, glcn, sorbitol, sucrose, cellulose, or starch. the ...201424682877
production of hexaoligochitin from colloidal chitin using a chitinase from aeromonas schubertii.chitin derivatives, such as those with modified main saccharide chains and deacetylated side chains, exhibit versatile biological functions. the biomedical properties of chitin oligosaccharides depend on their degree of oligomerization. of the chitin oligosaccharides, chitin hexamers are generally the most potent. in our recent study, n-acetylchitohexaose was obtained by digesting chitin with aschi61, a chitinase from aeromonas schubertii. in this work, the factors involved in the production of ...201424854211
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