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immune suppression in vivo with antigen-modified syngeneic cells. ii. t cell-mediated nonresponsiveness to fowl gamma-globulin.intravenous administration of syngeneic spleen cells coupled with the palmitoyl derivative of fowl gammma-globulin (p-f gamma g) results in a profound state of f gamma g-specific tolerance in c57bl/6 mice. administration of p-f gamma g coupled syngeneic cells specifically reduces both the primary and secondary hapten and carrier-specific pfc responses to tnp-f gamma g. since the haptenic response is affected, the tolerance functions at the level of the f gamma g-specific helper t cell. as few as ...197987447
cytotoxic macrophages induced in hamsters by vaccinia virus: selective cytotoxicity for virus-infected targets by macrophages collected late after immunization. 197987476
response to immunological or cholinergic stimulation of isolated mast cells from man, guinea pig and rat. 197991954
analysis of complement factor h mrna expression: dexamethasone and ifn-gamma increase the level of h in l cells.murine complement protein h is encoded by a 100-kb gene on chromosome 1. a 3.2-kb fragment of the 5' flanking region of the h gene was sequenced, and two transcription start sites for this gene were identified by rnase protection and s1 nuclease analyses, each of which had upstream tata and caat boxes. this region shares sequence homology with known regulatory elements, including the sv40 enhancer consensus, the sp1 binding site, and two glucocorticoid-responsive core elements (gre). tissue and ...19892533512
oviducal storage of spermatozoa in the turkey: its relevance to artificial insemination technology.1. this paper addresses questions concerning the role of oviducal sperm selection and storage in the relative inefficiency of turkey reproduction. 2. before the onset of egg production, the turkey has the biological potential to store spermatozoa in its oviduct for periods up to 5 weeks; after the onset of production fewer spermatozoa are retained by the oviduct. 3. a better understanding of the biological mechanisms which regulate sperm selection and storage in the turkey oviduct is necessary t ...19892670069
rates of cholesterol synthesis and low-density lipoprotein uptake in the adrenal glands of the rat, hamster and rabbit in vivo.the absolute rate of cholesterol acquisition from de novo synthesis and from receptor-dependent and receptor-independent low-density lipoprotein (ldl) uptake was determined in the adrenal glands of the rat, hamster and rabbit under in vivo conditions. the rate of incorporation of [3h]water into cholesterol in the adrenal gland was much higher in the hamster (1727 nmol/h per g) and rabbit (853 nmol/h per g) than in the rat (71 nmol/h per g). assuming that 23 atoms of 3h are incorporated into the ...19852992599
a procedure of vestibular decompensation for clinical diagnosis. i. animal experiments.vestibular lesions are often so effectively compensated that their diagnosis may be difficult. certain chemicals can decompensate the central vestibular system and thus uncover symptoms present before compensation. this study attempts to devise a procedure which can utilize such decompensation for the benefit of clinical diagnosis. unilateral labyrinthectomies were performed in rats. pathological movements and body positions due to the vestibular loss were compensated within about one week. syst ...19863485723
[plasmids of azospirillum brasilense].the cells from natural isolates of a. brasilense were found to harbour 1 to 4 plasmids with the molecular masses within the 27-300 md range. 100 md plasmids are specific for this bacterial species. strains isolated from the roots of cereals (wheat, maize, barley) have more heterogeneous plasmid composition as compared to the strains isolated from soil.19853939568
molecular mechanisms of olfactory reception. vi. kinetic characteristics of camphor interaction with binding sites of rat olfactory epithelium.camphor binding to a possible receptor of rat olfactory epithelium has been studied within the ligand concentration range 10(-11)-10(-6) m. at these concentrations camphor is bound by a set of receptors. they are distinguished by both the affinity to the ligand (k1 = 5 x 10(-10) m, k2 = 3.5 x 10(-8) m, k3 approximately equal to 10(-6) m) and their amount in the epithelium. the differences in the affinities are due to different values of the association rate constant of camphor (k1), which varies ...19853986222
[probability in the evaluation of the reliability of bactericidal effects]. 19854029632
applications of kinetic methods to aminoacyl-trna synthetases. 19744368426
[interferon production by fragments in suspension of monkey organs]. 19664959477
chronic diarrhea due to a single strain of aeromonas caviae.over a period of 17 months, aeromonas caviae was cultured 15 times as the sole enteropathogen from the feces of a man who had developed chronic diarrhea after traveling to turkey. determination of rrna gene restriction patterns confirmed that the seven isolates of aeromonas caviae studied were identical (hybridization group [hg]4). after therapy with ciprofloxacin for four weeks, the patient was culture negative for the original isolate, and six months later a novel strain of aeromonas media wit ...19957537217
isolation of a novel strain of aeromonas media producing high levels of dopa-melanin and assessment of the photoprotective role of the melanin in bioinsecticide applications.to isolate a bacterium that produces high yield of melanin and to examine the effect of this bacterial pigment on the efficacy of a bioinsecticide.200718045437
expanded-spectrum beta-lactamase per-1 in an environmental aeromonas media isolate from switzerland. 200818644966
molecular and biochemical characterization of a distinct tyrosinase involved in melanin production from aeromonas media.a new tyrosinase was isolated from aeromonas media strain ws and purified to homogeneity. the purified tyrosinase, termed tyra, had a molecular mass of 58 kda and an isoelectric point of 4.90. it exhibited optimal monophenol and diphenol oxidase activities under basic conditions (ph>8.0). tyra had a relatively higher affinity to diphenol substrate l-dihydroxyphenylalanine (l-dopa) than many other tyrosinases. edta or glutathione notably inhibited the enzymatic activities of tyra, whereas triton ...200918931836
classification of brown pigmented aeromonads isolated from river water.nine aeromonas strains having a brown exopigment were isolated during the microbiological examination of river water. these brown-pigmented aeromonads were characterized by the phenotyping, fatty-acid methyl-ester analysis and ribotyping. all methods identically confirmed that the group of brown-pigmented aeromonads is quite heterogeneous. apart from the aeromonas media taxon, the brown-pigmented aeromonads in river water were represented also by strains of a. allosaccharophila and a. salmonicid ...200919418249
bacteriocin activity and probiotic activity of aeromonas media.three strains of aeromonas media (161, a164 and a199) were shown to be active in-vitro producers of bacteriocin-like inhibitory substances (blis). for example, the producer strain, aer. media a199, displayed antagonistic activity against all strains tested of aer. caviae, aer. hydrophila, aer. salmonicida, aer. veronii var. sobria, listonella anguillarum, photobacterium damsella, eight species of vibrio and yersinia ruckeri. because of this wide-ranging activity against fish/shellfish pathogens, ...199821182714
Diversity and antibiotic resistance of Aeromonas spp. in drinking and waste water treatment plants.The taxonomic diversity and antibiotic resistance phenotypes of aeromonads were examined in samples from drinking and waste water treatment plants (surface, ground and disinfected water in a drinking water treatment plant, and raw and treated waste water) and tap water. Bacteria identification and intra-species variation were determined based on the analysis of the 16S rRNA, gyrB and cpn60 gene sequences. Resistance phenotypes were determined using the disc diffusion method. Aeromonas veronii pr ...201121907383
association between chloramphenicol, hypoplastic anemia and acute myeloblastic leukemia: a case report. 20164981395
studies on pure red cell aplasia. vi. development of two-stage erythroblast cytotoxicity method and role of complement. 20144123985
examination of patients for prosthesis: especially the aging patients. 20154568882
dental specialties--past, present and future. 20154444815
calcaneal gait in spastic diplegia after heel cord lengthening: a study with gait analysis.calcaneal gait or deformity can be a significant complication after heel cord lengthening. after heel cord lengthening, 20 children with spastic diplegia were evaluated by gait analysis to define calcaneal gait objectively and describe associated morbidity. mean age was 5 years 2 months (range 2 years 7 months to 8 years 2 months), and mean length of follow-up was 5 years 8 months (range 1 years 1 month 11 year 3 months). calcaneal gait was defined as dorsiflexion 1 sd beyond the mean in the sag ...20082600179
aaee minimonograph #32: the electrophysiologic examination in patients with radiculopathies.a brief history of the evolution of radiculopathy as a clinical entity, and the use of electrodiagnostic studies to diagnose it, are provided. root anatomy and the concept of myotomes and dermatomes are reviewed, as is the pathophysiology of radiculopathy. the value and limitations of the various electrophysiologic procedures used in the diagnosis of radiculopathies are discussed, including motor and sensory nerve conduction studies, late responses, somatosensory evoked potentials, nerve root st ...20103067085
aaee minimonograph #32: the electrophysiologic examination in patients with radiculopathies.a brief history of the evolution of radiculopathy as a clinical entity, and the use of electrodiagnostic studies to diagnose it, are provided. root anatomy and the concept of myotomes and dermatomes are reviewed, as is the pathophysiology of radiculopathy. the value and limitations of the various electrophysiologic procedures used in the diagnosis of radiculopathies are discussed, including motor and sensory nerve conduction studies, late responses, somatosensory evoked potentials, nerve root st ...20103067085
criteria for a recommended standard occupational exposure to hot environments. i. recommendations for a standard for work in hot environments. 20154704229
temporal and contextual cues as discriminative attributes in retardates' recognition memory. 20154704210
temporal and contextual cues as discriminative attributes in retardates' recognition memory. 20154704210
our experiences of cystic fibrosis and what we expect from the paediatrician. 20133656343
data entry errors in computerized nutritional calculations. 20113088246
[interphase death of lymphoid cells: role in the genesis of radiation sickness and molecular mechanisms].an analysis of the data on the effect of lymphoid cells on the proliferation and differentiation of hemopoietic stem cells has led to a conclusion that radiation injury of lymphocytes plays an important role in the pathogenesis of the cerebrospinal syndrome. the molecular mechanisms of lymphocyte interphase death were considered. it was shown that due to some peculiarities in the energy supply of these cells the appearance of breaks in dna causes the development of biochemical processes resultin ...20133657448
[properties of lak cell activity and lak-generation in pbl isolated from patients with renal cell carcinoma]. 20123266646
a word about words. 20123459834
[burn scars of the lower extremities and chronic venous insufficiency]. 20123407224
[platelets, adp, camp, and antiplatelet drugs]. 20154366039
[method of detection of bacterial mutations induced by cosmic rays]. 20144159300
identification and epidemiological relationships of aeromonas isolates from patients with diarrhea, drinking water and foods.a collection of aeromonas isolates obtained over a three-year period in the same geographic area (león, nw of spain) was characterized by (gtg)(5)-pcr fingerprinting, amplified fragment length polymorphism (aflp) analysis and gyrb gene sequence analysis. the isolates originated from human diarrheal stools (29 isolates), potable water (13 isolates), rabbit meat (13 isolates) and marine fish (5 isolates). the distribution of aeromonas species varied with the strain source. aeromonas caviae hg4 and ...201121550680
occurrence, abundance and elimination of class 1 integrons in one municipal sewage treatment plant.integrons are elements that encode a site-specific recombination system that recognizes and captures mobile gene cassettes and are closely related to multiple resistances of environmental microorganisms. this study was undertaken to determine the efficiency of an activated sludge process to remove integrons. the prevalence and characteristics of class 1 integrons were investigated for bacterial species isolated from the activated sludge of nanjing jiangxinzhou sewage treatment plant (stp, china) ...201121431316
mechanisms of quinolone resistance in aeromonas species isolated from humans, water and eels.mechanisms of resistance were determined in 33 quinolone-resistant isolates of the species aeromonas hydrophila, aeromonas caviae, aeromonas media, aeromonas salmonicida, aeromonas popoffii and aeromonas veronii, recovered from humans, freshwater and eels. the quinolone resistance-determining regions (qrdrs) of gyra and parc genes were sequenced in these resistant strains, as well as in 8 quinolone-sensitive aeromonas used as controls. all quinolone-resistant aeromonas carried point mutations in ...201019900538
[characterization of aeromonas spp isolates from newborns hospitalized].aeromonas spp is recognized as pathogenic to humans after consumption of contaminated water and food. in the present investigation, 2,323 rectal swab samples from newborns hospitalized in rio de janeiro were evaluated with a view to isolating aeromonas. the samples were collected and sent to the national reference laboratory for cholera and other bacterial intestinal infections, at the oswaldo cruz institute of the oswaldo cruz foundation. the swabs were subjected to enrichment in alkaline pepto ...200818545840
temperate bacteriophage phio18p from an aeromonas media isolate: characterization and complete genome sequence.a group of 74 aeromonas isolates from surface water of three ponds in bielefeld, germany was screened for prophage induction after uv irradiation. the phage phio18p was induced from the aeromonas media isolate o18. phio18p belongs to the myoviridae phage family. the complete nucleotide sequence of the double stranded dna genome of bacteriophage phio18p consists of 33,985 bp. the genome has 5' protruding cohesive ends of 16 bases. on the phio18p genome 46 open reading frames (orfs) were identifie ...200818096197
antagonistic activity of aeromonas media strain a199 against saprolegnia sp., an opportunistic pathogen of the eel, anguilla australis richardson.a bacteriocin-like inhibitory substance (blis) produced by aeromonas media strain a199 inhibited the growth of saprolegnia sp. in vitro, an opportunistic pathogen isolated from affected eels, anguilla australis (richardson). the presence of blis in solid media inhibited the growth of the vegetative state of the aquatic mould as well as the germination of cysts. uninhibited growth was, however, observed in the presence of inactive blis, suggesting that the in vitro antagonism derived from the bli ...200312962224
epidemiological relationships between aeromonas strains isolated from symptomatic children and household environments as determined by ribotyping.ribotyping was used to study the epidemiology of aeromonas associated gastro-enteritis in young children. ribotyping patterns of 29 aeromonas strains (16 aeromonas caviae, 8 aeromonas hydrophila, 3 aeromonas eucrenophila, 1 aeromonas veronii, and 1 aeromonas encheleia) isolated from primary stool cultures of sick children were compared using the gelcompare software with patterns of 104 strains (39 aeromonas eucrenophila, 29 aeromonas caviae, 11 aeromonas encheleia, 10 aeromonas hydrophila, 6 aer ...200010997832
a putative heat-labile enterotoxin expressed by strains of aeromonas media.fifteen isolates of aeromonas media (seven from diarrhoeal stools, four from water and four from superficial skin ulcers of catfish) were examined for enterotoxin production. ten of these isolates (six diarrhoeal, one from water and three from fish) caused accumulation of fluid in the initial rabbit ileal loop (ril) tests. isolates from diarrhoeal stools and fish caused relatively more fluid accumulation than those from water. those strains that caused little or no fluid accumulation in the init ...200010933251
inactivation of biofilm bacteria.the current project was developed to examine inactivation of biofilm bacteria and to characterize the interaction of biocides with pipe surfaces. unattached bacteria were quite susceptible to the variety of disinfectants tested. viable bacterial counts were reduced 99% by exposure to 0.08 mg of hypochlorous acid (ph 7.0) per liter (1 to 2 degrees c) for 1 min. for monochloramine, 94 mg/liter was required to kill 99% of the bacteria within 1 min. these results were consistent with those found by ...20102849380
prevention of suicide phenomenon in aeromonads.clinical isolates of aeromonas (13 aeromonas caviae), 8 aeromonas hydrophila, 3 aeromonas spp., and 2 aeromonas media recovered from diarrheal feces of children were submitted to the suicide phenomenon test and investigated at intervals of 24 h for up to 120 h. the same isolates were also stimulated by repeated passages in broth for ten days before the test. after the bacterial stimulus, a decreases in the number of aeromonads with suicidal capacity was observed. the suicide phenomenon was expre ...19968793406
[changes in the enzymatic activity of the mucosa of the small intestine in rats after administration of neomycin and rhodomycin]. 19854069837
evaluation of different conditions and culture media for the recovery of aeromonas spp. from water and shellfish samples.to perform a comparative study for determining the optimum culture method (direct plating or enrichment) and medium (ampicillin dextrin agar (ada), starch ampicillin agar (saa), bile salts irgasan brilliant green modified (bibg-m)) for recovering aeromonas species from water and shellfish samples.201627333573
sugar absorption in healthy preterm and full-term infants.we have studied carbohydrate absorption in 40 healthy term infants and 10 preterm neonates (31-35 weeks gestation) by respiratory h2, fecal ph, and chromatographic analysis of stools. sequential studies of h2 excretion (24-h collection) in response to breast feeding were carried out in premature infants during the first 8 weeks of life. five expired h2 during the first 2 weeks, and two continued to do so in the 3rd to 4th weeks. breath h2 excretion fell below 10 ppm by 8 weeks and was not relate ...19863958849
successful treatment of post-mitral valve annuloplasty aspergillus flavus endocarditis.aspergillus endocarditis is associated with a very high mortality. of approximately 67 cases reported in the english language literature, there have been only two known survivors. this report describes a patient with aspergillus flavus endocarditis after mitral valve annuloplasty who recovered with combined surgical and antifungal therapy. this is the first successfully treated case due to a. flavus and the first involving an annuloplasty ring.19853934969
gas-liquid chromatographic analysis of cellular fatty acid methyl esters in aeromonas species.the cellular fatty acids of 39 strains belonging to the genus aeromonas (aeromonas hydrophila, aeromonas caviae, aeromonas sobria, aeromonas media, aeromonas schubertii, aeromonas veronii) were determined by high resolution gas-liquid chromatography. the fatty acid profiles were characterized by major amounts (60% or more) of one saturated (hexadecanoic acid = 16:0) and two unsaturated (hexadecenoic acid = 16:1 and octadecenoic acid = 18:1) acids. while the majority of the strains of the six spe ...19911930556
homage to professor alfred meyer on his 94th birthday. 20103067111
darkroom diseases and how to combat them. 20113112384
waste water effluent contributes to the dissemination of ctx-m-15 in the natural environment.multidrug-resistant enterobacteriaceae pose a significant threat to public health. we aimed to study the impact of sewage treatment effluent on antibiotic resistance reservoirs in a river.201424797064
draft genome sequence of high-melanin-yielding aeromonas media strain ws.we sequenced the genome of the high-melanin-yielding aeromonas media strain ws and then analyzed genes potentially involved in melanin formation. the 4.2-mb draft genome carries multiple genes responsible for pyomelanin synthesis and other candidate genes identified in our separate study, which have no homolog in other strains of aeromonas species.201223144426
[difficulties in the diagnosis of epilepsy in children].on the basis of an analysis of 3 cases it was shown that the occurrence of seizure-like symptoms with abnormal eeg and history data suggesting possible damage to the central nervous system are not always sufficient for an unequivocal diagnosis of epilepsy and for beginning of long-term antiepileptic treatment which is not without adverse effects on the developing organism of a child. for establishing of correct diagnosis and avoiding of too hasty introduction of anticonvulsant treatment not only ...20123587512
[difficulties in the diagnosis of epilepsy in children].on the basis of an analysis of 3 cases it was shown that the occurrence of seizure-like symptoms with abnormal eeg and history data suggesting possible damage to the central nervous system are not always sufficient for an unequivocal diagnosis of epilepsy and for beginning of long-term antiepileptic treatment which is not without adverse effects on the developing organism of a child. for establishing of correct diagnosis and avoiding of too hasty introduction of anticonvulsant treatment not only ...20123587512
comparison of intravenous ranitidine & cimetidine on gastric acid secretion & ph. 20144054988
[various physiological and biochemical peculiarities in selective phytotoxicity of triazines in viran infection]. 20051214640
low-dose diuretic therapy for hypertension.the efficacy of low dosages of diuretics was evaluated in two studies. in one, 62 (48%) of 130 patients became normotensive with 2.5 mg/day of metolazone. in the other, 28 (49%) of 57 patients became normotensive with 25 mg of chlorthalidone, compared with 12 (22%) of 55 patients given placebo. there was a marked variation in blood pressure response and the occurrence of hypokalemia (less than 3.5 meq/l of potassium) from center to center and within patient groups in both studies. the mean decre ...20113768929
[surgery and diabetes mellitus (author's transl)]. 20154467806
effects of reticuloendothelial blockade on acute dimethylnitrosamine poisoning in mice. 20154438333
visual information obtained from flashes and from afterimages. 20164771206
fetal supradiaphragmatic accessory liver lobe. report of a case and review of the literature.a right thoracic accessory liver lobe with a normal diaphragm is reported in a stillborn fetus. a review of the literature reveals seven cases affecting adult patients.2003150113
fourth pharyngeal pouch sinus.this is the first description and documentation of an extremely rare developmental anomaly of the pharyngeal apparatus in a 15-year-old female. the clinical, radiological, histological, and anatomical findings indicated that the anomaly originated in the pharyngeal fourth pouch. it presented as a recurrent lateral neck abscess which was due to a paratracheal sinus. this sinus penetrated the larynx and after a translaryngeal course ended in the pyriform sinus. it was caudad to the superior laryng ...20144033337
role of surgical treatment in pancreatic carcinoma.from 1964 through 1984, we treated 810 patients for carcinoma of the pancreas. in 129 patients pde was possible (16%). up to 1977, we had performed whipple's procedures, while from 1978 through 1982 total pancreatectomy was our principal procedure. since 1983, partial pde again has been the procedure of choice for the potentially curative treatment of pancreatic cancer. comparing the results of both methods (60 partial/64 total), we did not find any advantages of total pancreatectomy. operative ...20123815874
[personality of patients with myocardial infarct]. 20051236645
biometeorological triggers in childhood asthma.the relationship between child asthma admissions and biometeorological factors was investigated over a 16-month period. no relationship was found with many commonly suspected precipitants such as temperature, humidity and wind. there was however a strong association with rainfall (p much less than 0.001). associations occurred with low barometric pressure and counts of coloured basidiospores and green algae, but no significant relationship was found with grass pollen.20123416418
[liver function in patients with chronic ischemic heart disease combined with peptic ulcer]. 20123493589
[surgical sciatica. study of 251 cases undergoing a single operation (author's transl)]. 2005544204
identification and molecular characterization of the homogentisate pathway responsible for pyomelanin production, the major melanin constituents in aeromonas media ws.the pigmentation of many aeromonas species has been thought to be due to the production of a l-dopa (l-3,4-dihydroxyphenylalanine) based melanin. however, in this study we found that although l-dopa synthesis occurs in the high-melanin-yielding aeromonas media strain ws, it plays a minor, if any, role in pigmentation. instead, the pigmentation of a. media strain ws is due to the production of pyomelanin through hga (homogentisate). gene products of phha (encodes phenylalanine hydroxylase), tyrb ...201525793756
the interpretation of psychic reality, developmental influences, and unconscious communication. 20144056299
efficacy of lactoferricin b in controlling ready-to-eat vegetable spoilage caused by pseudomonas spp.the microbial content of plant tissues has been reported to cause the spoilage of ca. 30% of chlorine-disinfected fresh vegetables during cold storage. the aim of this work was to evaluate the efficacy of antimicrobial peptides in controlling microbial vegetable spoilage under cold storage conditions. a total of 48 bacterial isolates were collected from ready-to-eat (rte) vegetables and identified as belonging to acinetobacter calcoaceticus, aeromonas media, pseudomonas cichorii, pseudomonas flu ...201526453993
antimicrobial resistance and plasmid profile of bacterial strains isolated from the urbanized eltsovka-1 river (russia).antimicrobial resistance and plasmid profile of gram-positive and gram-negative bacterial strains isolated from the urbanized eltsovka-1 river (russia) were investigated. sequencing of the 16s rrna of of g+ strains showed 99-100% identity to that of bacillus aerophilus, bacillus altitudinis, bacillus amyloliquefaciens, bacillus anthrancis, bacillus barbaricus, bacillus cereus, bacillus flexus, bacillus indriensis, bacillus stratosphericus, bacillus subtilis subsp. subtilis, bacillus thuringiensi ...201525803299
draft genome sequences of amoeba-resistant aeromonas spp. isolated from aquatic environments.amoeba-resistant aeromonas veronii arb3 and aeromonas media arb13 and arb20, which may be important intracellular pathogens of eukaryotic hosts, were isolated from pond and river waters. the draft genome sequences indicate that the strains harbor multiple protein secretion systems and toxins that induce disruption of the actin cytoskeleton.201425359918
enhanced production of recombinant aspartase of aeromonas media nfb-5 in a stirred tank reactor.aspartase gene (aspa) from aeromonas media nfb-5 was cloned and expressed in escherichia coli bl21 using pet21b(+) expression vector. maximum production of aspartase was obtained at shake-flask after 5 h of iptg (1.5 mm) induction at 37°c and by supplementing the media with kh2po4 (0.3%, w/v) and k2hpo4 (0.3%, w/v). further production was investigated at a laboratory scale stirred tank reactor using response surface methodology (rsm). agitation (130-270 rpm), aeration (0.30-1.70 vvm) and iptg in ...201323219690
broad diversity of conjugative plasmids in integron-carrying bacteria from wastewater environments.in this study we assessed the occurrence, diversity and conjugative potential of plasmids in integron-carrying aeromonas and enterobacteriaceae from wastewaters. sixty-six strains were included as donors in mating assays using rifampicin-resistant escherichia coli and pseudomonas putida recipient strains. the diversity of plasmids from donors and transconjugants (resistant to tetracycline or streptomycin) was evaluated by restriction analysis and replicon typing targeting 19 incompatibility grou ...201222409355
single-step purification and characterization of recombinant aspartase of aeromonas media nfb-5.aspartase (l-aspartate ammonia-lyase; ec 4.3.1.1) catalyzes the reversible amination of fumaric acid to produce l-aspartic acid. aspartase coding gene (aspa) of aeromonas media nfb-5 was cloned, sequenced, and expressed with his tag using pet-21b⁺ expression vector in escherichia coli bl21. higher expression was obtained with iptg (1.5 mm) induction for 5 h at 37 °c in lb medium supplemented with 0.3% k₂hpo₄ and 0.3% kh₂po₄. recombinant his tagged aspartase was purified using ni-nta affinity chr ...201222328292
delineation of taxonomic species within complex of species: aeromonas media and related species as a test case.aeromonas media is an opportunistic pathogen for human and animals mainly found in aquatic habitats and which has been noted for significant genomic and phenotypic heterogeneities. we aimed to better understand the population structure and diversity of strains currently affiliated to a. media and the related species a. rivipollensis. forty-one strains were included in a population study integrating, multilocus genetics, phylogenetics, comparative genomics, as well as phenotypics, lifestyle, and ...201728458658
malignant melanoma. 20103768643
a spectrophotometric study of the interaction of deoxyribonucleoproteins with the acriflavine-phosphotungstic acid complex. 20144214994
phylogenetic diversity, antibiotic resistance and virulence traits of aeromonas spp. from untreated waters for human consumption.it is well known that water constitutes an important contamination route for microorganisms. this is especially true for aeromonas which are widespread in untreated and treated waters. in this study, portuguese untreated waters not regularly monitored were screened for the presence and diversity of aeromonads. a total of 206 isolates were discriminated by rapd-pcr and 80 distinct strains were identified by gyrb based phylogenetic analysis. the most frequently detected species were aeromonas hydr ...201223107502
aeromonas rivipollensis sp. nov., a novel species isolated from aquatic samples.two gram-negative, facultatively anaerobic, motile and rod-shaped bacteria, strains p2g1(t) and p1a11, were isolated from the ter river in ripoll, spain. phylogenetic analysis based on 16s rrna gene sequences showed that both strains are closely related to each other and that their closest relatives were aeromonas media atcc 33907(t) (99.4%) and aeromonas hydrophila atcc 7966(t) (99.3%). multilocus sequence analysis (mlsa) based on the partial sequences of gyra, gyrb, rpod, reca, and dnaj genes ...201526630331
semen collection methods affect the bacterial composition of post-thawed semen of silver barb (barbodes gonionotus).biosafety issue associated with the risk of pathogenic contamination of cryopreserved semen is a common concern because of associated declines in sperm quality, storage period and disease transmission. this study was conducted to evaluate the effects of methods of semen collection on sperm quality and bacterial composition of post-thawed semen of silver barb (barbodes gonionotus). semen collection methods consisted of four treatments: (1) hand-stripping of abdomen without rinsing of urogenital a ...201626778122
the occurrence of aeromonas in drinking water, tap water and the porsuk river.the occurrence of aeromonas spp. in the porsuk river, public drinking water and tap water in the city of eskisehir (turkey) was monitored. fresh water samples were collected from several sampling sites during a period of one year. total 102 typical colonies of aeromonas spp. were submitted to biochemical tests for species differentiation and of 60 isolates were confirmed by biochemical tests. further identifications of isolates were carried out first with the vitek system (biome˜rieux) and then ...201124031613
oxa-427, a new plasmid-borne carbapenem-hydrolysing class d β-lactamase in enterobacteriaceae.to describe a novel plasmid-borne class d carbapenemase (chdl) named oxa-427 identified in several enterobacteriaceae clinical isolates from nine patients in one belgian hospital.201728859446
identification of yfih and the catalase cata as polyphenol oxidases of aeromonas media and cata as a regulator of pigmentation by its peroxyl radical scavenging capacity.pyomelanin is the major constituent of pigment in melanogenic aeromonas strains of bacteria. however, eumelanin, synthesized from tyrosine via l-dopa and polyphenol oxidases (ppos), may also be present in this genus since l-dopa is frequently detected in culture fluids of several species. to address this question, we used a deletion mutant of aeromonas media strain ws, in which pyomelanin synthesis is completely blocked under normal culture conditions. when tyrosine was supplied to the medium, w ...201729051758
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