hemolysins of edwardsiella tarda.isolates of edwardsiella tarda from four sources produced nonfilterable hemolsin in trypticase soy broth. the cell-associated hemolysin was partially heat labile, destroyed by formalin and sensitive to treatment with trypsin. these characteristics, and the observation that ca++ or mg++ ions enhanced activity, suggest that a proteinaceous, enzymic component may be responsible for the hemolytic activity.197934473
demonstration of an alpha2-macroglobulin receptor in human fibroblasts, absent in tumor-derived cell lines. 197987392
the role of p23,30-bearing human macrophages in antigen-induced t lymphocyte responses. 197988270
passive transfer in diabetes mellitus. 197988629
cell differentiation in human gastric gland as revealed by nuclear binding of tritiated actinomycin.the nuclear binding of h3 actinomycin, which is closely linked to the differentiation phenomenon, was studied in human normal gastric mucosa. actinomycin binding decreases in cells which differentiate and becomes very low in fully differentiated cells. in the gastric pits, there is a decreasing gradient of labelling from the deeper stem cells to the well-differentiated superficial cells. this indicates that migration and renewal of the surface epithelium occurs following a 'pipe-line' system. al ...197990638
[ultrastructural cytochemistry of the mouse juxtaglomerular apparatus (author's transl)].the ultrastructural cytochemistry of the mouse juxtaglomerular apparatus has been studied. the specific granules of the juxtaglomerular cells were found to be argentaphobic when ultrathin sections of araldite-embedded renal cortex were stained according to the periodic acid-thio-carbohydrazide-silver proteinate technique of thiery. this technique revealed an abundant glycogen of type beta in the specific granules and in the cytosol of these cells. the rim of specific granules was positive when u ...197992002
relations between statistical properties of spontaneous activity of lateral geniculate neurons and their anatomo-physiological features [proceedings]. 197992234
development of visual acuity in infant monkeys (macaca nemestrina) during the early postnatal weeks. 197896592
clinical relevance of the liver-specific lipoprotein (lsp). 197896648
catecholamine-induced alterations in glucose homeostasis in baboons, dogs, rabbits, and rats: comparative effects of somatostatin. 197898685
changes in composition of tears of malnourished children. 197898738
[isolation of edwardsiella tarda from duodenal juice and feces of a patient with chronic cholangitis (author's transl)]. 1978101616
the fate of transplanted pancreatic islets in the rat.syngeneic pancreatic islets transplanted into the liver or the spleen reverse streptozotocin-induced diabetes in the rat, but allogeneic islets function only briefly and are rejected. shortly after transplantation, thrombi often form around transplanted tissue, particularly around nonislet tissue that contaminates islet preparations. these thrombi are a source of transient liver injury in recipients of intrahepatic grafts. a few days after transplantation, syngeneic islets injected into the port ...1979104625
transcription and translation in a pleiotropic streptomycin-resistant mutant of escherichia coli.the role of the ribosomal protein s12 (streptomycin protein) in ribosome function and in other metabolic processes in the cell has been investigated. a spontaneous streptomycin-resistant strain of escherichia coli (sm3) carrying a mutation in the rpsl gene is deficient in its ability to induce the synthesis of the enzyme bets-galactosidase. it was demonstrated that the reduced rate of enzyme synthesis results from deficiencies in both the transcription of the lactose operon and translation of th ...1979104958
homology of the gene coding for outer membrane lipoprotein within various gram-negative bacteria.the mrna for a major outer membrane lipoprotein from escherichia coli was found to hybridize specifically with one of the ecori and one of the hindiii restriction endonuclease-generated fragments of total dna from nine bacteria in the family enterobacteriaceae: e. coli, shigella dysenteriae, salmonella typhimurium, citrobacter freundii, klebsiella aerogenes, enterobacter aerogenes, edwardsiella tarda, serratia marcescens, and erwinia amylovora. however, among the enterobacteriaceae, dna from two ...1979104972
heterologous radioimmunoassay for rabbit prolactin.a highly specific heterologous double-antibody ria has been developed to measure rabbit prl by using guinea pig antiserum to human prl and ovine [125 i]iodo-prl. rabbit pituitary prl and serum give parallel dose-response curves in the assay and no cross-reaction (less than 0.1%) occurs with gh, placental lactogens, lh, fsh, or tsh from several different species. the assay is suitable for the measurement of human, ovine, bovine, caprine, and canine prl in addition to rabbit prl, but shows no cros ...1978105878
the role of catecholamines in clinical medicine.the sympathetic nervous system is of major importance for the regulation of several physiological functions. drugs which inhibit the actions of catecholamines and adrenergic drugs are used in the treatment of many clinical disorders. the potential role of catecholamines in a number of human diseases has, however, until recent years been studied to a limited extent only due to lack of methods for quantitation of sympathetic nervous activity. after the development of enzymatic isotope-derivative a ...1979106629
a new principle of injectable depot contraceptives. i drug selection and studies in monkeys.the aim of the investigations was to develop a long-acting depot contraceptive on the basis of norethisterone or levonorgestrel. disappointing results with levonorgestrel nonanoate and levonorgestrel undecylate showed that elongation of the fatty acid, esterified with the steroid, decreased the bioavailability of the latter due to incomplete hydrolysis of the ester. therefore, several new compounds were synthesized which contained a bifunctional molecule between the steroid and the fatty acid. i ...1979110513
effect of neonatal hypothyroidism and delayed l-triiodothyronine treatment on behavioural activity and norepinephrine and dopamine biosynthetic systems in discrete regions of rat brain. 1979111295
renal malakoplakia with renal vein thrombosis. 1979116513
the role of 5-ht in the mechanism of amphetamine stereotypy-inducing action was potentiated in rats in proportion to the previously administered doses of pcpa (3 x 100, 3 x 150 or 3 x 200 mg/kg); however, the dose of 3 x 300 mg/kg pcpa brought about the decrease in stereotypy. reserpinized rats exhibited also increased am stereotypy but of shorter duration. am hyperactivity in mice was potentiated also by the pretreatment with pcpa but this action occurred within a further phase of observation (30 min after the injection). it seems then tha ...1975128744
cerebral aterial spasm. i. adrenergic mechanism in experimental cerebral vasospasm.this study demonstrates that an adrenergic mechanism plays an important role in producing the delayed cerebral vasospasm which follows subarachnoid hemorrhage. results were as follows: 1. experimental subarachnoid hemorrhage (sah) was produced by injection of fresh arterial blood into the cisterna magna in cats. the cerebral vasospasm was shown angiographically to be biphasic in nature: immediate constriction lasting 1 h and marked prolonged spasm occurring between the 3rd and 5th day after sah. ...1978148833
[immunoprofile of lmif in immunologic deficiency syndromes. ii. mediator production by isolated subpopulations of human lymphocytes]. 1978149303
adenine arabinoside for therapy of herpes zoster in immunosuppressed patients: preliminary results of a collaborative study.eighty-seven immunosuppressed patients (53 with localized and 34 with disseminated herpes zoster) from 10 institutions were enrolled in a controlled therapeutic trial of adenine arabinoside. a crossover design was employed; thus, 47 patients received drug for five days and then received placebo, and 40 were given the two substances in the opposite order. resolution of acute pain and the cutaneous lesions were graded during a 10-day observation period. during the initial five-day period, treated ...1976180198
subcellular distribution of steroids in rat adrenocortical tissue after incubation in vitro [proceedings]. 1977194992
regulation of the in vitro anamnestic antibody response by cyclic amp. ii. antigen-dependent enhancement by exogenous prostaglandins of the e series. 1978212208
suppressive effects on simian virus 40-induced oncogenesis of several immunosuppressive agents and hormonal modifications applied during the latent period.the long latent period required for tumor induction with simian virus 40 (sv40) in the subcutis of hamsters can be used to investigate whether host factor(s) participate in oncogenesis. several treatments were applied during this period, and the results were compared with those of the same treatment applied in another series of experiments, homologous sv40 tumor grafting in hamsters. the results obtained were as follows: (a) the latent period for sv40 tumor induction in the female was shorter th ...1978214231
identification and characterization of normal and malignant human blood leukocytes by surface glycoprotein patterns.we have labeled surface glycoproteins of normal and malignant human blood leukocytes by the galactose oxidase-nab3h4 and periodate-nab3h4 labeling techniques. the labeled glycoproteins were separated by slab gel electrophoresis in the presence of sodium dodecyl sulfate and visualized by fluorography. the different types of normal blood cells could be distinguished by their surface glycoprotein patterns. the surface glycoproteins of cells from patients with acute lymphoblastic, myeloid, or monobl ...1978291363
detection of r plasmids in naturally occurring fish-pathogenic bacteria, edwardsiella tarda.the conjugative r plasmids were detected from fish-pathogenic edwardsiella tarda isolated from cultured eels. these r plasmids were resistant to sulfonamide and tetracycline, or sulfonamide, streptomycin, chloramphenicol, tetracycline and kanamycin. there were no inhibitions in growth of phages w-31, p1, t1, t3, t7, and lambda. they were classified into incompatibility group a.1977323647
pharmacological control of reproduction cycles. 1979380121
[isolation of yersinia pseudotuberculosis and edwardsiella tarda from a dead monitor lizard (varanus niloticus)]. 1977406717
edwardsiella tarda in freshwater catfish and their environment.edwardsiella tarda was isolated from 47, 88, and 79% of skin, visceral, and dressed-fish samples, respectively. this species was also isolated from 30% of imported dressed fish, 75% of catfish pond water samples, 64% of catfish pond mud samples, and 100% of frogs, turtles, and crayfish from catfish ponds. the incidence of edwardsiella increased during the summer months, as water temperatures increased. of several isolation media evaluated, the most effective was selective enrichment in double-st ...1979539822
enterobacteria of emerging pathogenic significance from clinical cases in man and animals and detection of toads and wall lizards as their reservoirs.a total of 416 samples comprising faecal samples from diarrhoeic cases of man, calves, sheep and goats, and urine samples from patients with urinary tract infections, were examined for the presence of enterobacteria of emerging pathogenic significance. citrobacter freundii from 20, c. intermedius biotype-a from four, serratia marcescens (serotype 05:h13, bactericin type 16) from one and erwinia herbicola from two human stool samples were isolated. only two urine samples yielded c. freundii. citr ...1978582096
edwardsiellosis in man and animals in panamá: clinical and epidemiological characteristics.edwardsiella tarda was isolated during etiologic and epidemiologic investigations of diarrheal disease agents in man and for enterobacteriaceae in various species of wild-caught animals in panamá. a total of 50 strains were recovered from approximately 14,000 specimens cultured between 1965 and 1972. in this period, edwardsiella was isolated from ten individuals with a clinically diagnosed diarrheal syndrome, while 20 of some 3,000 persons from rural areas were asymptomatic carriers of these org ...1977596515
occurrence of edwardsiella tarda in the household lizard, gecko gecko. 1977604577
some interesting isolates from a diagnostic laboratory.citrobacter koseri, plesiomonas shigelloides, edwardsiella tarda, yersinia enterocolitica, alkalescens dispar, vibrio parahaemolyticus, and vibrio alginolyticus were seven interesting microorganisms isolated recently in our diagnostic laboratory.1978641196
intestinal bacterial flora of the household lizard, gecko gecko.a total of 114 isolates was recovered from the intestines of 43 househould lizards, gecko gecko. among the important ones were staphylococcus aureus, salmonella typhimurium, pseudomonas aeruginosa, proteus mirabilis and edwardsiella tarda.1978653125
isolation of edwardsiella tarda from three oregon sea mammals.edwardsiella tarda was isolated from the peritoneal exudate of a steller's sea lion (eumetopias jubata) with peritonitis resulting from a perforating colonic ulcer; from the liver of a harbor porpoise (phocena phocena) with metritis and peritonitis sequela to dystocia; and from the liver of a california sea lion (zalophus californianus) with peritonitis following fracture and necrosis of a lumbar vertebrae. these findings indicate e. tarda is a common opportunistic invader in sick or injured mar ...1978691128
[childhood gastroenteritis caused by edwardsiella tarda]. 1978741022
edwardsiella tarda associated with osteomyelitis.a case of osteomyelitis associated with edwardsiella tarda is reported. specimens from a wound exudate of a 58-year-old female with osteomyelitis revealed on a direct gram-stained smear, pleomorphic, gram-negative rods. growth of the organism on differential media and analysis of its analytab (api)(*) patterns produced results consistent with e. tarda. the identification of the organism was confirmed by the center for disease control, atlanta. this represents the first reported case of a wound i ...1977753935
formation of crystalline deposits by several genera of the family enterobacteriaceae.several species of bacteria from the family enterobacteriaceae formed crystalline materials containing calcium when grown in a defined culture medium. enterobacter aerogenes, proteus vulgaris, citrobacter freundii, and c. intermedius produced calcium pyrophosphate crystals. edwardsiella tarda and escherichia coli formed calcite iii crystals, whereas proteus mirabilis, klebsiella pneumoniae, providencia stuartii, and serratia marcescens produced hydroxyapatite crystals. several of these bacteria ...1976786887
[distribution of edwardsiella tarda and hydrogen sulfide-producing escherichia coli in healthy persons]. 1976816980
isolation of salmonellae and other potential pathogens from the freshwater aquarium snail ampullaria.the freshwater aquarium snail (ampullaria spp.) was demonstrated to carry as many as 10(8) viable mesophilic bacteria per g of meat plus shell. some 16 genera of bacteria were identified, with gram negatives predominating. enrichment culture techniques enabled the isolation of salmonellae from 24 to 42 lots of 200 g each. the salmonellae comprised eight different serotypes, including salmonella newport, salmonella saint-paul, and salmonella infantis. this association of salmonellae with snails m ...1976818954
[microbiological pollution of the environment due to breeding. i. enterobacteriaceae due to cattle breeding].enterobacteriaceae from faeces of cattle belonging to four cattle farms situated in the ferrara district were investigated. at the same time, investigation was made of effluent sewage and recipient wells (upstream and downstream). the cattle (of the italian frisona breed) resulted uncontaminated by salmonellae, but proved to be very susceptible to arizona, citrobacter, shigella and s. gallinarum-pullorum infections, coming from the environment. predominant species in faeces were as follows: prot ...1977911464
[enterobacteria of reptiles (author's transl)].the aerobic gram-negative faecal flora of 78 reptiles consisting of 46 species (39 lizards of 23 species, 15 tortoises of 9 species, 24 snakes of 14 species) was studied. salmonella was found to be present in 50% of lizards, in 16% of tortoises and in 16% of snakes. there were all together 15 different serotypes. edwardsiella tarda was isolated in 20% of tortoises, in 12% of snakes but only in 3% of lizards. tortoises represent therefore the possible normal habitat of edwardsiella tarda. we isol ...1976998035
edwardsiella tarda in a variety of human infections. 19761024075
[the acute vestibular paralysis (author's transl)].acute vestibular paralysis may not be considered as a nosologic entity but as a syndrome. symptomatology (vertigo, spontaneous and provoked vestibular nystagmus, absence of cochlear signs) shows an uniform picture. the results of the caloric test as well as the nystagmic responses induced by galvanic stimulation and the development of central vestibular compensation however indicate that the site of the lesion is not only confined to the labyrinth but may also occur at the level of the periphera ...19751081389
intussusception in a florida intussusception resulting from an embedded fishhook and a mass of nylon cord, monofilament line, and wire was determined to be the cause of death in a florida manatee (trichechus manatus latirostris). pathologic findings are given along with information on bacteria (edwardsiella tarda), pesticide residues (dde, pcb's and dieldrin), and parasites (chiorchis fabaceus).19751195505
edwardsiella tarda infections in florida racoons, procyon lotor.edwardsiella tarda was isolated from the large intestine of seven (17%) of 42 racoons from florida. the rate varied from 12% in south florida to 25% in north florida. in addition, 52% of the racoons examined were carrying salmonella, with numerous serotypes represented.19751200720
[nutrition and taxonomy of "enterobacteriaceae" and related bacteria. iii. nutritional characters and differentiation of the taxonomic groups (author's transl)].a batch of 186 strains belonging to the families enterobacteriaceae or vibrionaceae has been studied by determination, for each strain, of the "versatility" towards 146 organic substrates tested as sole source of carbon and energy. this study allowed to work out a classification of these strains into the 32 classes which have been previously described. in the present paper the nutritional characters of these classes are reported. on the basis of these characters, the 32 classes may be regrouped ...19751217784
[contribution to the study of "edwardsiella tarda" isolated in viet-nam (author's transl)].rectal swabs from 13,947 diarrhea patients of all ages were examined during the five years period covered by this report (1969-1973). most of the specimens came from the saigon area, mainly from the infectious diseases hospital and the children's hospital. specimens also were submitted from other hospitals at provinces. from these specimens, 186 edwardsiella tarda were isolated for a 8.77 0/0 isolation rate. these organisms were relatively frequent among adults and more commonly isolated during ...19751243748
edwardsiella tarda bacteremia.presented are two case reports of patients with edwardsiella bacteremia who survived after chloramphenicol and gentamicin therapy in one case and cephalothin and kanamycin therapy in the other case. of four previously reported patients, only one survived. one of our two patients presented with subarachnoid hemorrhage associated with bacterial endocarditis and, to our knowledge, this is the first reported case of e tarda endocarditis. the clinical and laboratory features of disease due to this un ...19761251242
a puncture wound complicated by infection with edwardsiella tarda. 19921361947
classification of strains of pragia fontium, budvicia aquatica and of leminorella by whole-cell protein pattern.sds page protein patterns of 37 h2s-positive strains of species belonging to the family enterobacteriaceae including the genera budvicia (11 strains) and leminorella (l. grimontii--3 strains, l. richardii--4 strains) were compared with 10 strains of species pragia fontium. all strains under study form well separated clusters with overall similarity c = .49. clusters are separated in the range of c = .68-.83. they display high homogeneity, only one strain of edwardsiella tarda clusters with budvi ...19921512457
infection with edwardsiella tarda related to a vascular prosthesis. 19921554858
inhibition of elevated hepatic glutathione abolishes copper deficiency cholesterolemia.dietary copper deficiency causes hypercholesterolemia and increased hepatic 3-hydroxy-3-methyl-glutaryl coenzyme a (mhg-coa) reductase activity and increased hepatic glutathione (gsh) in rats. we hypothesized that inhibition of gsh production by l-buthionine sulfoximine (bso), a specific gsh synthesis inhibitor, would abolish the cholesterolemia and increased hmg-coa reductase activity of copper deficiency. in two experiments, two groups of 20 weanling male rats were fed diets providing 0.4 and ...19921563598
lack of benefit of ursodeoxycholic acid in drug-induced cholestasis in the rat.the administration of ursodeoxycholic acid (udca) has been reported to improve cholestasis in patients with primary biliary cirrhosis or sclerosing cholangitis. in the present study, we tested the hypothesis that udca similarly might reduce cholestasis induced by drugs. rats were treated with three different drugs reported to induce cholestasis: 17 alpha-ethynylestradiol, alpha-napthylisothiocyanate, and cyclosporine a. udca administration (0.4.g/day-1.k-1 before and during administration of the ...19921570353
successful medical management of a patient with multiple hepatic abscesses due to edwardsiella tarda.isolation of edwardsiella tarda in humans has been associated with an asymptomatic carrier state as well as mild, self-limited diarrheal illness. extraintestinal manifestations have included soft-tissue infections, meningitis, osteomyelitis, cholangitis, and sepsis. only three cases of patients who had documented hepatic abscess due to e. tarda have been reported in the english-language literature; two patients died, and the third required a laparotomy and drainage. we report what is, to our kno ...19921571414
[an electron microscopic study of the interaction of bacterial intestinal microflora and rotavirus virions].transmission electron microscopy has revealed the capacity of rotaviruses for adsorption on escherichia coli, klebsiella pneumoniae, proteus mirabilis cells and the absence of such capacity with respect to enterococcus faecalis, lactobacillus casei and edwardsiella tarda. different degrees and a varying character of the adsorption of rotaviruses by the representatives of the opportunistic group of bacteria have been established, which may aggravate the course of rotavirus diarrhea due to the agg ...19911661985
edwardsiella tarda infection in a puppy with possible parvovirus infection. 19911662426
comparison of the effects of hydralazine and nifedipine on contractions and 45ca influx of rat aorta.the effect of the vasodilator hydralazine has been compared with nifedipine on kcl-(k+) (60 mm) and noradrenaline-(na) (10 microm) induced 45ca uptake and contractile responses in rat aorta arterial strips without endothelium. hydralazine (0.5-10 mm) was equally effective in relaxing k(+)- (ic50 = 2.2 +/- 0.17 mm) and na- (ic50 = 3.06 +/- 0.25 mm) induced tension, the degree of relaxation depending on the dose. nifedipine totally inhibited k(+)- (ic50 = 3.16 +/- 0.28 nm) induced contractions wit ...19911680180
pathogenic properties of edwardsiella species.the pathogenic characteristics of 35 edwardsiella strains from clinical and environmental sources were investigated. overall, most edwardsiella tarda strains were invasive in hep-2 cell monolayers, produced a cell-associated hemolysin and siderophores, and bound congo red; many strains also expressed mannose-resistant hemagglutination against guinea pig erythrocytes. edwardsiella hoshinae strains bound congo red and were variable in their invasive and hemolytic capabilities while edwardsiella ic ...19911774326
the effect of exposure to impulse x rays on normal and epileptic activity in rabbit brain.exposure to x rays (20 impulses of 4 hz frequency, total dose 0.6-1.1 mgy) increased the epileptic activity of a focal area, which was produced in the visual cortex of rabbit brain by freezing with liquid nitrogen, and by stimulating with flashes of light at frequencies of 5-6 hz. the number of seizure complexes during photostimulation for 5 s increased by 80% compared with the initial level, and this effect continued for 15 min. in control animals (with no epileptic foci), a decrease was observ ...19911797142
the effect of exposure to impulse x rays on normal and epileptic activity in rabbit brain.exposure to x rays (20 impulses of 4 hz frequency, total dose 0.6-1.1 mgy) increased the epileptic activity of a focal area, which was produced in the visual cortex of rabbit brain by freezing with liquid nitrogen, and by stimulating with flashes of light at frequencies of 5-6 hz. the number of seizure complexes during photostimulation for 5 s increased by 80% compared with the initial level, and this effect continued for 15 min. in control animals (with no epileptic foci), a decrease was observ ...19911797142
antimutagenic and anticarcinogenic effects of betel leaf extract against the tobacco-specific nitrosamine 4-(n-nitrosomethylamino)-1-(3-pyridyl)-1-butanone (nnk).earlier studies showed that betel leaf inhibits the mutagenic action of standard mutagens like benzo[a]pyrene and dimethylbenz[a]anthracene. since tobacco-specific nitrosamines are the major carcinogens present in unburnt forms of tobacco, we studied the effect of an extract of betel leaf on the mutagenic and carcinogenic actions of one of the most potent, 4-(n-nitrosomethylamino)-1-(3-pyridyl)-1-butanone (nnk). betel-leaf extract and hydroxychavicol suppressed the mutagenicity of nnk in both th ...19911855909
in vitro susceptibilities of edwardsiella tarda to 22 antibiotics and antibiotic-beta-lactamase-inhibitor agents.the in vitro susceptibilities of 22 isolates of edwardsiella tarda were studied with 22 antibiotics and antibiotic-beta-lactamase-inhibitor agents. results indicated that all isolates were susceptible to the aminoglycosides, cephalosporins, penicillins, imipenem, aztreonam, ciprofloxacin, and antibiotic-beta-lactamase-inhibitor agents. each strain produced a beta-lactamase even though no resistance was detected to the beta-lactams.19911873976
health status of salmonids in river systems in natal. iii. isolation and identification of bacteria.both pathogenic and non-pathogenic bacteria were isolated from fish, both salmonid and non-salmonid, from selected river systems in natal. pasteurella pisicida was isolated for the first time from fish in south africa. the isolation of yersinia ruckeri, aeromonas salmonicida, and edwardsiella tarda were recorded for the first time from fish in natal. a. hydrophila and flexibacter columnaris were found to be widespread throughout the river systems in natal. the streptococcus species which caused ...19911881658
bacteria associated with tegument of clinostomum marginatum (digenea).adults of clinostomum marginatum freshly collected from a heron, ardea herodias, were examined using transmission electron microscopy. specimens from the mouth of the bird were encrusted with bacteria that were not removed by washing unless the saline contained antibiotics. there was no evidence that the attached bacteria were damaging to the trematode tegument. three species of gram-negative bacteria were isolated from the worm surfaces and identified; achromobacter sp. was present in pure cult ...19911919930
edwardsiella tarda: a causative agent in human a retrospective study 45 specimens of e. tarda infection from 44 adult cases at songklanagarind hospital during february 1982 to march 1989 were reviewed. there were 24 males and 20 females, with a mean age of 48.20 years. nearly all of e. tarda were isolated from extraintestinal sources, especially pus and urine and most of them were subsequently found to be nosocomial-acquired infections. about half were polomicrobial infections of e. tarda and gram negative bacilli. forty one patients were ...19911948259
[pansinusitis, brain abscess and subdural empyema caused by an unusual microorganism: edwardsiella tarda]. 19901969672
penetration and replication of edwardsiella spp. in hep-2 cells.the ability of 22 edwardsiella strains to penetrate and replicate in cultured epithelial cells was initially evaluated by light microscopy methods and by the recovery of gentamicin-resistant (gmr) bacteria from the triton x-100 cell lysates of hep-2-infected monolayers. giemsa-stained hep-2 cells revealed the presence of numerous internalized bacteria 3 h postinfection, often appearing as parallel rows of replicated bacteria within the cytosol and sometimes obliterating the cytoplasm because of ...19911987028
[reactions of different enterobacteriaceae and vibrionaceae species in brila-mug (fluorocult) bouillion].in order to test the usefulness of brila-mug (= fluorocult) medium (merck) for isolation and identification of total coliforms and faecal coliforms in surface water according to the ec guidelines for bathing waters a total of 969 strains of different enterobacteriaceae and vibrionaceae species was examined under different culture conditions. these included 486 e. coli (reference strains of o-groups 1-170, enterotoxin and verotoxin-producing strains), 149 salmonella (subspecies i-vi), 92 yersinia ...19902080970
["edwardsiella tarda" in marine animals]. 19902131605
kinetics of the reaction of prostaglandin h synthase compound ii with ascorbic acid.the reduction of prostaglandin h synthase compound ii by ascorbic acid in the presence of diethyldithiocarbamate was studied in 0.1 m phosphate buffer (ph 8.0) at 4.0 +/- 0.5 degrees c, by rapid scan spectrometry and transient state kinetics. a saturation effect and nonzero intercept were observed in the plot of pseudo-first-order rate constant versus ascorbic acid concentration. the saturation behavior suggests formation of a complex between prostaglandin h synthase compound ii and ascorbic aci ...19902168160
[a case of sepsis caused by edwardsiella tarda complicated panophthalmitis and pyogenic spondylitis].edwardsiella tarda (e. tarda) is gram negative enterobacteriaceae which has been found generally in animal hosts and occasionally in human feces. we have reported a case of sepsis caused by e. tarda, complicated panophthalmitis and pyogenic spondylitis. a 39-year old patient suffered from fever, polyarthralgia and lumbago. we performed blood culture, from which e. tarda was isolated. spinal ct scan showed destruction and osteogenesis of the fourth and fifth lumbar vertebral body and cranial ct s ...19902212756
new animal breeding techniques and their application.the new biotechnologies of gene transfer, in-vitro production, cloning and sexing of embryos have been developed and are being refined with efficiencies suitable for use in animal agriculture. efficient in-vitro systems for maturing oocytes and capacitating spermatozoa, for fertilizing and developing the embryos have resulted in commercial in-vitro production of embryos. cloning of embryos by nuclear transfer has been accomplished for sheep, cattle, pigs and rabbits with nuclear material supplie ...19902213713
life-threatening edwardsiella tarda soft-tissue infection associated with catfish puncture wound. 19902230281
toxicological evaluation of morning glory seed: subchronic 90-day feeding study.diets containing 0.8, 2.53 and 8.0% field variety morning glory seed were fed to male and female rats (20 per group) in a 90-day subchronic feeding study. gross clinical observations, body weight, and feed and water intake were recorded weekly. at 90 days, all surviving rats were autopsied, organs were weighed, and blood chemistry analyses, haematology, and bone-marrow evaluation for evidence of clastogenic effects were performed. tissues from control (0% seed) and high-dose (8.0% seed) rats wer ...19902242829
homology among bacterial catalase genes.catalase activities in crude extracts of exponential and stationary phase cultures of various bacteria were visualized following gel electrophoresis for comparison with the enzymes from escherichia coli. citrobacter freundii, edwardsiella tarda, enterobacter aerogenes, klebsiella pneumoniae, and salmonella typhimurium exhibited patterns of catalase activity similar to e. coli, including bifunctional hpi-like bands and a monofunctional hpii-like band. proteus mirabilis, erwinia carotovora, and se ...19902253114
the isolation and identification of edwardsiella tarda from a diseased native australian eel (anguilla reinhardtii). 19902264808
participation of graft and host brain dendrites in the establishment of interconnections. 19902290942
soft-tissue infection caused by edwardsiella tarda and aeromonas hydrophila. 19902319177
differences in the phagocytosis of four bacteria by channel catfish neutrophils.the purpose of this study was to compare the phagocytosis of aeromonas hydrophila, edwardsiella ictaluri, edwardsiella tarda and micrococcus luteus by channel catfish neutrophils. various aspects of opsonization effect and bactericidal ability of channel catfish neutrophils were investigated. percent phagocytosis ranged from a low of 1% to a high of 91%. the highest percent phagocytosis and phagocytic indices routinely occurred with normal serum and were highest for m. luteus (91.78%, 55.25) and ...19902369968
a cluster of edwardsiella tarda infection in a day-care center in florida. 19902388007
aminergic neurotransmitter and water content changes in rats after transient forebrain ischemia.we have studied changes of cerebral monoamine metabolism and water content, during recirculation following global transient ischemia (20 min) using the four-vessel occlusion model in rats. levels of monoamines and their metabolites were determined in cortex, striatum, hippocampus, and hypothalamus. water content was evaluated by weight and by the analysis of t1 and t2 relaxation times in 1h-nuclear magnetic resonance. norepinephine levels decreased; 3,4-dihydroxyphenylethylamine, 3,4-dihydroxyph ...19862430056
aminergic neurotransmitter and water content changes in rats after transient forebrain ischemia.we have studied changes of cerebral monoamine metabolism and water content, during recirculation following global transient ischemia (20 min) using the four-vessel occlusion model in rats. levels of monoamines and their metabolites were determined in cortex, striatum, hippocampus, and hypothalamus. water content was evaluated by weight and by the analysis of t1 and t2 relaxation times in 1h-nuclear magnetic resonance. norepinephine levels decreased; 3,4-dihydroxyphenylethylamine, 3,4-dihydroxyph ...19862430056
time of insemination and its effect on in-vitro fertilization, cleavage and pregnancy rates in gnrh agonist/hmg-stimulated this prospective study, we compared the effect of delayed inseminations on fertilization, cleavage and pregnancy rates in two groups of patients. in one group, the ovarian stimulation was performed with a clomiphene citrate/human menopausal gonadotrophin/human chorionic gonadotrophin (cc/hmg/hcg) protocol. the other group was pre-treated with gonadotrophin-releasing hormone agonist (gnrha) and ovarian stimulation was carried out with an hmg/hcg protocol. under both protocols, a delay of 2, 4 ...19892515208
recombinant human erythropoietin treatment in patients on maintenance home haemodialysis. 19892570273
effect of urapidil on the performance of ischaemic myocardium in anaesthetized dogs.the effect of urapidil on the ischaemic myocardium was studied in eight anaesthetized dogs. stenosis of the left descending coronary artery reduced blood flow and systolic contraction of the post-stenotic myocardium by about 50%; the end-diastolic length of the post-stenotic myocardium and the end-diastolic pressure increased, while aortic pressure slightly decreased. subsequent administration of urapidil (0.25 + 0.25 + 0.5 + 1.0 mg/kg intravenously) did not affect the systolic shortening and en ...19892576670
[the electrolyte balance of the blood in dogs exposed to multiple +gz overloads].experiments of the series were carried out using 7 noninbred dogs. in the first series, the animals were exposed to + gz acceleration of increasing value once or twice a week during 5 months. each exposure continued until distinct cardiac arrhythmias were seen in ecg. two weeks after the onset of exposure the dogs displayed hyponatremia and higher k-na coefficient and two months later they showed hyperkalemia and hypermagnesemia which led to a 1.5 g increase of acceleration tolerance. in the sec ...19892586060
serious infections with edwardsiella tarda. a case report and review of the literature.edwardsiella tarda, a member of the family enterobacteriaceae, has recently become recognized as pathogenic, especially in patients with an underlying illness. in the present report, a patient had sickle cell hemoglobinopathy and e tarda bacteremia. other cases of serious infection with edwardsiella are reported in the literature. edwardsiella infection may present as bacteremia, enteric fever, gastroenteritis, localized infection, and an asymptomatic carrier state. on the basis of this review, ...19892643415
automated biochemical identification of bacterial fish pathogens using the abbott quantum ii.the quantum ii, originally designed by abbott diagnostics for automated rapid identification of members of enterobacteriaceae, was adapted for the identification of bacterial fish pathogens. the instrument operates as a spectrophotometer at a wavelength of 492.600 nm. a sample cartridge containing 20 inoculated biochemical chambers is inserted in the path of the analyzing beam. reactions are converted into a 7-digit octal biocode, relayed via a sensor to the memory module, and compared to biocod ...19892644450
dexamethasone regulation of the expression of cytokine mrnas induced by interleukin-1 in the astrocytoma cell line u373mg.bsf-2/il-6, gm-csf and il-1 beta mrnas were induced by recombinant il-1 in human astrocytoma cell line u373mg. the induction of bsf-2/il-6 and il-1 beta mrnas did not require de novo protein synthesis while that of gm-csf mrna required a newly synthesized protein. dexamethasone inhibited the induction of these cytokine mrnas by il-1. this process seems to require continued protein synthesis. these results suggest that the production of these cytokines are positively and negatively controlled by ...19892646146
cytoskeletal proteins including myofilaments in human tumors. 19892660368
immunocytochemical localization and immunochemical characterization of an insulin-related peptide in the pancreas of the urodele amphibian, ambystoma mexicanum.the pancreas of the axolotl, ambystoma mexicanum, was investigated by immunocytochemical methods for the presence of immunoreactivity to a number of antisera raised against mammalian insulins. all anti-insulin antisera tested revealed substantial amounts of reaction products confined solely to the aldehyde-fuchsinophilic b cells of the endocrine pancreas. the reactive cell population was detected by use of one polyclonal antiserum against bovine insulin and eight different monoclonal antibodies ...19892663173
a new tool for the serodiagnosis of acute plasmodium falciparum malaria in individuals with primary infection.we have developed an elisa which detects, with high specificity, antibodies against a major surface protein of p. falciparum merozoites which is a processing product of the precursor glycoprotein gp190. this assay can be used in the diagnosis of acute malaria in individuals with primary infection. two partial sequences of gp190 were expressed in e. coli as beta-galactosidase (beta-gal) fusion proteins. the same sequences fused to chloramphenicol acetyltransferase (cat) or mouse dihydrofolate red ...19892668417
comparison of direct immunofluorescent staining and an enzyme-linked immunoabsorbent assay for identifying chlamydia trachomatis in cervical and urethral specimens.a direct fluorescent antibody (dfa) stain (microtrak, syva) and an enzyme-linked immunoabsorbent assay (chlamydiazyme, abbott) were used to detect chlamydial infection in 179 cervical specimens and 56 specimens obtained from the female urethra. chlamydiazyme, when compared with the dfa technique, revealed a high degree of correlation in these specimens (22 and 21 positive cervical specimens, respectively, and 7 each positive urethral specimens). since both techniques are readily available, inexp ...19892681749
electrical and mechanical properties of spontaneous contraction in hypertensive rat portal vein.contractile and electrical activities of longitudinal smooth muscle of portal vein from normotensive wistar kyoto rats (wky) and stroke-prone spontaneously hypertensive rats (shrsp) were compared. amplitude and duration of spontaneous contraction of shrsp portal vein were greater than those of wky portal vein. no significant differences were observed in the resting membrane potentials between these preparations. spontaneous spike activity appeared as a form of bursts. duration of the burst and t ...19892726435
surface properties and ultrastructure of edwardsiella species.twenty-two strains of edwardsiella representing the three currently recognized species were evaluated for surface characteristics and ultrastructural morphology. all isolates tested possessed a high surface cell charge as detected by deae-cellulose chromatography; surface hydrophobicity was variable and strain but not species dependent. two major types of adhesins detected by hemagglutination assays were identified: one was inhibited by d-mannose (mannose-sensitive hemagglutinin) and found on al ...19892768468
[cardiovascular risk factors in children and adolescents in fuenlabrada: objectives, features of the population, design, participation, equipment, laboratory technics, and quality control].fuenlabrada is at the present time the most industrialized town placed at the south are of madrid. this population has some peculiar attractive characteristics from the epidemiologic point of view. perhaps the most important is that the majority of its inhabitants are immigrant from other depressed rural areas of the country, with a low socio-economic and cultural level. the new conditions of life could have changed the life expectation and the causes of morbidity and mortality of this populatio ...19892772364
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