fimbriae and haemagglutinating properties in edwardsiella and levinea.using the haemagglutinating procedure, the presence of fimbriae was revealed in all 34 edwardsiella strains tested and in 3 levinea strains out of 17 examined. the great majority of fimbriate edwardsiella strains caused haemagglutination of mr type; only two strains of edwardsiella and all fimbriate strains of levinea brought about haemagglutination of ms type. all the strains exhibited a similar spectrum of haemagglutinating activity when they were tested against red cells of various animal spe ...197770969
[isolation of edwardsiella tarda from duodenal juice and feces of a patient with chronic cholangitis (author's transl)]. 1978101616
homology of the gene coding for outer membrane lipoprotein within various gram-negative bacteria.the mrna for a major outer membrane lipoprotein from escherichia coli was found to hybridize specifically with one of the ecori and one of the hindiii restriction endonuclease-generated fragments of total dna from nine bacteria in the family enterobacteriaceae: e. coli, shigella dysenteriae, salmonella typhimurium, citrobacter freundii, klebsiella aerogenes, enterobacter aerogenes, edwardsiella tarda, serratia marcescens, and erwinia amylovora. however, among the enterobacteriaceae, dna from two ...1979104972
[evaluation of a new medium for detection of salmonella, arizona and edwardsiella]. 1977405437
[isolation of yersinia pseudotuberculosis and edwardsiella tarda from a dead monitor lizard (varanus niloticus)]. 1977406717
enterobacteriaceae infections in man and animals and detection of their natural reservoirs in india.the study presents a summary of the results regarding the presence of enterobacteriaceae in domestic animals and birds, endeavouring to explain the origin of the infections. wild reptiles, amphibians, mammalia, rodents and birds were examined into the demonstration of enterobacteriaceae, whereby particular attention was paid to the following species: arizona, edwardsiella, citrobacter, enterobacter, serratia, yersinia and erwinia.1979452773
edwardsiella tarda in freshwater catfish and their environment.edwardsiella tarda was isolated from 47, 88, and 79% of skin, visceral, and dressed-fish samples, respectively. this species was also isolated from 30% of imported dressed fish, 75% of catfish pond water samples, 64% of catfish pond mud samples, and 100% of frogs, turtles, and crayfish from catfish ponds. the incidence of edwardsiella increased during the summer months, as water temperatures increased. of several isolation media evaluated, the most effective was selective enrichment in double-st ...1979539822
enterobacteria of emerging pathogenic significance from clinical cases in man and animals and detection of toads and wall lizards as their reservoirs.a total of 416 samples comprising faecal samples from diarrhoeic cases of man, calves, sheep and goats, and urine samples from patients with urinary tract infections, were examined for the presence of enterobacteria of emerging pathogenic significance. citrobacter freundii from 20, c. intermedius biotype-a from four, serratia marcescens (serotype 05:h13, bactericin type 16) from one and erwinia herbicola from two human stool samples were isolated. only two urine samples yielded c. freundii. citr ...1978582096
edwardsiellosis in man and animals in panamá: clinical and epidemiological characteristics.edwardsiella tarda was isolated during etiologic and epidemiologic investigations of diarrheal disease agents in man and for enterobacteriaceae in various species of wild-caught animals in panamá. a total of 50 strains were recovered from approximately 14,000 specimens cultured between 1965 and 1972. in this period, edwardsiella was isolated from ten individuals with a clinically diagnosed diarrheal syndrome, while 20 of some 3,000 persons from rural areas were asymptomatic carriers of these org ...1977596515
some interesting isolates from a diagnostic laboratory.citrobacter koseri, plesiomonas shigelloides, edwardsiella tarda, yersinia enterocolitica, alkalescens dispar, vibrio parahaemolyticus, and vibrio alginolyticus were seven interesting microorganisms isolated recently in our diagnostic laboratory.1978641196
intestinal bacterial flora of the household lizard, gecko gecko.a total of 114 isolates was recovered from the intestines of 43 househould lizards, gecko gecko. among the important ones were staphylococcus aureus, salmonella typhimurium, pseudomonas aeruginosa, proteus mirabilis and edwardsiella tarda.1978653125
[childhood gastroenteritis caused by edwardsiella tarda]. 1978741022
edwardsiella tarda associated with osteomyelitis.a case of osteomyelitis associated with edwardsiella tarda is reported. specimens from a wound exudate of a 58-year-old female with osteomyelitis revealed on a direct gram-stained smear, pleomorphic, gram-negative rods. growth of the organism on differential media and analysis of its analytab (api)(*) patterns produced results consistent with e. tarda. the identification of the organism was confirmed by the center for disease control, atlanta. this represents the first reported case of a wound i ...1977753935
formation of crystalline deposits by several genera of the family enterobacteriaceae.several species of bacteria from the family enterobacteriaceae formed crystalline materials containing calcium when grown in a defined culture medium. enterobacter aerogenes, proteus vulgaris, citrobacter freundii, and c. intermedius produced calcium pyrophosphate crystals. edwardsiella tarda and escherichia coli formed calcite iii crystals, whereas proteus mirabilis, klebsiella pneumoniae, providencia stuartii, and serratia marcescens produced hydroxyapatite crystals. several of these bacteria ...1976786887
[distribution of edwardsiella tarda and hydrogen sulfide-producing escherichia coli in healthy persons]. 1976816980
isolation of salmonellae and other potential pathogens from the freshwater aquarium snail ampullaria.the freshwater aquarium snail (ampullaria spp.) was demonstrated to carry as many as 10(8) viable mesophilic bacteria per g of meat plus shell. some 16 genera of bacteria were identified, with gram negatives predominating. enrichment culture techniques enabled the isolation of salmonellae from 24 to 42 lots of 200 g each. the salmonellae comprised eight different serotypes, including salmonella newport, salmonella saint-paul, and salmonella infantis. this association of salmonellae with snails m ...1976818954
serologic relationship of fimbriae among enterobacteriaceae.the existence of serological relationship of fimbriae among serotypes of the same genus and among genera of enterobacteriaceae was determined by agglutination and absorption procedures. it was found that fimbriae of different serotypes of the same genus and among the genera a) escherichia, shigella and klebsiella, and b) salmonella, arizona and citrobacter were more or less antigentically related. no relationship was found among fimbriae of the edwardsiella, enterobacter, hafnia, serratia, prote ...1977880000
[microbiological pollution of the environment due to breeding. i. enterobacteriaceae due to cattle breeding].enterobacteriaceae from faeces of cattle belonging to four cattle farms situated in the ferrara district were investigated. at the same time, investigation was made of effluent sewage and recipient wells (upstream and downstream). the cattle (of the italian frisona breed) resulted uncontaminated by salmonellae, but proved to be very susceptible to arizona, citrobacter, shigella and s. gallinarum-pullorum infections, coming from the environment. predominant species in faeces were as follows: prot ...1977911464
a simplified biochemical system to screen salmonella isolates from poultry for serotyping.the 24 most frequently isolated paratyphoids from poultry, along with salmonella gallinarum and salmonella pullorum, plus strains of arizona, citrobacter, edwardsiella, escherichia, klebsiella, prteus, pseudomonas, serratia and shigella were inoculated into triple sugar iron (tsi) and lysine iron (li) slants and into six fermentation broths which were numbered: 1 (dextrose), 2 (lactose), 3 (sucrose), 4 (mannitol), 5 (maltose), 6 (dulcitol). all the salmonella cultures (except s. pullorum) gave a ...1976995819
[enterobacteria of reptiles (author's transl)].the aerobic gram-negative faecal flora of 78 reptiles consisting of 46 species (39 lizards of 23 species, 15 tortoises of 9 species, 24 snakes of 14 species) was studied. salmonella was found to be present in 50% of lizards, in 16% of tortoises and in 16% of snakes. there were all together 15 different serotypes. edwardsiella tarda was isolated in 20% of tortoises, in 12% of snakes but only in 3% of lizards. tortoises represent therefore the possible normal habitat of edwardsiella tarda. we isol ...1976998035
[isolation of bacteria of the genus edwardsiella from patients with acute intestinal infections]. 19761015072
edwardsiella tarda in a variety of human infections. 19761024075
motility-indole-lysine medium for presumptive identification of enteric pathogens of enterobacteriaceae.detection of lysine decarboxylase activity is a useful supplement to reactions on triple sugar-iron (tsi) and urea agars in the initial examination of suspected pathogenic isolates from fecal cultures. owing to the added value of motility and indole production in the differentiation of enteric pathogens, we prepared and evaluated a motility-indole-lysine (mil) medium. the following 890 organisms were tested: 264 shigella, 2 edwardsiella, 182 salmonella enteritidis, 235 s. typhi, 3 arizona, 32 ye ...19751176632
intussusception in a florida intussusception resulting from an embedded fishhook and a mass of nylon cord, monofilament line, and wire was determined to be the cause of death in a florida manatee (trichechus manatus latirostris). pathologic findings are given along with information on bacteria (edwardsiella tarda), pesticide residues (dde, pcb's and dieldrin), and parasites (chiorchis fabaceus).19751195505
edwardsiella tarda infections in florida racoons, procyon lotor.edwardsiella tarda was isolated from the large intestine of seven (17%) of 42 racoons from florida. the rate varied from 12% in south florida to 25% in north florida. in addition, 52% of the racoons examined were carrying salmonella, with numerous serotypes represented.19751200720
[nutrition and taxonomy of "enterobacteriaceae" and related bacteria. ii. general results and classification (author's transl)].a study of 186 strains belonging to eleven genera of the family enterobacteriaceae and to three genera of the family vibrionaceae has been carried out in order to determine their "versatility" towards 146 organic substrates tested as sole source of carbon and energy. glucose was the only substrate used by all the strains; gluconate and glycerol were used by respectively 184 and 185 strains; 55 substrates were used by no one strains. the 90 substrates which were used by a fraction of the strains ...19751217783
[nutrition and taxonomy of "enterobacteriaceae" and related bacteria. iii. nutritional characters and differentiation of the taxonomic groups (author's transl)].a batch of 186 strains belonging to the families enterobacteriaceae or vibrionaceae has been studied by determination, for each strain, of the "versatility" towards 146 organic substrates tested as sole source of carbon and energy. this study allowed to work out a classification of these strains into the 32 classes which have been previously described. in the present paper the nutritional characters of these classes are reported. on the basis of these characters, the 32 classes may be regrouped ...19751217784
[contribution to the study of "edwardsiella tarda" isolated in viet-nam (author's transl)].rectal swabs from 13,947 diarrhea patients of all ages were examined during the five years period covered by this report (1969-1973). most of the specimens came from the saigon area, mainly from the infectious diseases hospital and the children's hospital. specimens also were submitted from other hospitals at provinces. from these specimens, 186 edwardsiella tarda were isolated for a 8.77 0/0 isolation rate. these organisms were relatively frequent among adults and more commonly isolated during ...19751243748
edwardsiella tarda bacteremia.presented are two case reports of patients with edwardsiella bacteremia who survived after chloramphenicol and gentamicin therapy in one case and cephalothin and kanamycin therapy in the other case. of four previously reported patients, only one survived. one of our two patients presented with subarachnoid hemorrhage associated with bacterial endocarditis and, to our knowledge, this is the first reported case of e tarda endocarditis. the clinical and laboratory features of disease due to this un ...19761251242
a puncture wound complicated by infection with edwardsiella tarda. 19921361947
classification of strains of pragia fontium, budvicia aquatica and of leminorella by whole-cell protein pattern.sds page protein patterns of 37 h2s-positive strains of species belonging to the family enterobacteriaceae including the genera budvicia (11 strains) and leminorella (l. grimontii--3 strains, l. richardii--4 strains) were compared with 10 strains of species pragia fontium. all strains under study form well separated clusters with overall similarity c = .49. clusters are separated in the range of c = .68-.83. they display high homogeneity, only one strain of edwardsiella tarda clusters with budvi ...19921512457
infection with edwardsiella tarda related to a vascular prosthesis. 19921554858
successful medical management of a patient with multiple hepatic abscesses due to edwardsiella tarda.isolation of edwardsiella tarda in humans has been associated with an asymptomatic carrier state as well as mild, self-limited diarrheal illness. extraintestinal manifestations have included soft-tissue infections, meningitis, osteomyelitis, cholangitis, and sepsis. only three cases of patients who had documented hepatic abscess due to e. tarda have been reported in the english-language literature; two patients died, and the third required a laparotomy and drainage. we report what is, to our kno ...19921571414
[an electron microscopic study of the interaction of bacterial intestinal microflora and rotavirus virions].transmission electron microscopy has revealed the capacity of rotaviruses for adsorption on escherichia coli, klebsiella pneumoniae, proteus mirabilis cells and the absence of such capacity with respect to enterococcus faecalis, lactobacillus casei and edwardsiella tarda. different degrees and a varying character of the adsorption of rotaviruses by the representatives of the opportunistic group of bacteria have been established, which may aggravate the course of rotavirus diarrhea due to the agg ...19911661985
edwardsiella tarda infection in a puppy with possible parvovirus infection. 19911662426
effect of temperature on the immune system of channel catfish (ictalurus punctatus)--ii. adaptation of anterior kidney phagocytes to 10 degrees c.1. anterior kidney phagocytes from channel catfish (ictalurus punctatus) exposed to 10 and 24 degrees c from 1 day to 5 weeks were assayed for phagocytic ability and respiratory burst activity to aeromonas hydrophila and edwardsiella ictaluri. 2. the results of this study indicated that phagocytosis in channel catfish remained partially functional at low temperature without adaptation, although partial suppression was observed. 3. adaptation to low temperature did lead to an improvement in the r ...19911685385
evolution of the ferric enterobactin receptor in gram-negative bacteria.using sodium dodecyl sulfate-polyacrylamide gel electrophoresis of iron-deficient and replete cell envelopes, 59fe-siderophore uptake studies, and western immunoblots and cytofluorimetric analyses with monoclonal antibodies (mabs), we surveyed a panel of gram-negative bacteria to identify outer membrane proteins that are structurally related to the escherichia coli k-12 ferric enterobactin receptor, fepa. antibodies within the panel identified fepa epitopes that are conserved among the majority ...19911717434
pathogenic properties of edwardsiella species.the pathogenic characteristics of 35 edwardsiella strains from clinical and environmental sources were investigated. overall, most edwardsiella tarda strains were invasive in hep-2 cell monolayers, produced a cell-associated hemolysin and siderophores, and bound congo red; many strains also expressed mannose-resistant hemagglutination against guinea pig erythrocytes. edwardsiella hoshinae strains bound congo red and were variable in their invasive and hemolytic capabilities while edwardsiella ic ...19911774326
a new plate medium for rapid presumptive identification and differentiation of enterobacteriaceae.a new selective differential agar medium for rapid presumptive identification of enterobacteriaceae from water and food samples is described (emx id agar). by a combination of fluorogenic and chromogenic substrates, the medium detects the presence of beta-d-glucuronidase, beta-d-galactosidase, beta-d-xylosidase, tryptophane deaminase and h2s; additionally, cytochrome-oxidase and indole production can be demonstrated. this medium provides an inexpensive means for simple and rapid presumptive iden ...19911777382
what is the diagnostic value of beta-d-glucuronidase (bdg) activity of bacteria using fluorocult ecd agar for their cultivation?a total of 1510 strains from 15 genera of gram-negative and gram-positive bacteria were studied. more than 94% of 327 escherichia coli strains showed beta-d-glucuronidase (bdg) activity. seventeen serotypes from 170 e. coli o serogroup representatives were negative. relationship between the existence of bdg positive and negative e. coli strains in the same serogroup or serotype has not been observed. the rate of bdg positivity was 42% among salmonella arizonae strains and 42.2% among shigella st ...19911805501
beta-d-glucuronidase (bdg) activity of gram-negative bacteria.bdg is an inducible enzyme that is encoded by the uida gene in escherichia coli. genetic sequences of this gene are present in most if not all e. coli strains regardless of the bdg phenotype. expression of bdg activity can be influenced by lactose-induced catabolite repression or genetic mutations. salmonella, shigella and yersinia strains frequently exhibit positive bdg reaction. bdg activity of strains belonging to genus edwardsiella, serratia, yersinia, vibrio, erwinia, alcaligenes, acinetoba ...19911817425
in vitro susceptibilities of edwardsiella tarda to 22 antibiotics and antibiotic-beta-lactamase-inhibitor agents.the in vitro susceptibilities of 22 isolates of edwardsiella tarda were studied with 22 antibiotics and antibiotic-beta-lactamase-inhibitor agents. results indicated that all isolates were susceptible to the aminoglycosides, cephalosporins, penicillins, imipenem, aztreonam, ciprofloxacin, and antibiotic-beta-lactamase-inhibitor agents. each strain produced a beta-lactamase even though no resistance was detected to the beta-lactams.19911873976
health status of salmonids in river systems in natal. iii. isolation and identification of bacteria.both pathogenic and non-pathogenic bacteria were isolated from fish, both salmonid and non-salmonid, from selected river systems in natal. pasteurella pisicida was isolated for the first time from fish in south africa. the isolation of yersinia ruckeri, aeromonas salmonicida, and edwardsiella tarda were recorded for the first time from fish in natal. a. hydrophila and flexibacter columnaris were found to be widespread throughout the river systems in natal. the streptococcus species which caused ...19911881658
bacteria associated with tegument of clinostomum marginatum (digenea).adults of clinostomum marginatum freshly collected from a heron, ardea herodias, were examined using transmission electron microscopy. specimens from the mouth of the bird were encrusted with bacteria that were not removed by washing unless the saline contained antibiotics. there was no evidence that the attached bacteria were damaging to the trematode tegument. three species of gram-negative bacteria were isolated from the worm surfaces and identified; achromobacter sp. was present in pure cult ...19911919930
edwardsiella tarda: a causative agent in human a retrospective study 45 specimens of e. tarda infection from 44 adult cases at songklanagarind hospital during february 1982 to march 1989 were reviewed. there were 24 males and 20 females, with a mean age of 48.20 years. nearly all of e. tarda were isolated from extraintestinal sources, especially pus and urine and most of them were subsequently found to be nosocomial-acquired infections. about half were polomicrobial infections of e. tarda and gram negative bacilli. forty one patients were ...19911948259
enteric infections associated with exposure to animals or animal products.the epidemiology and clinical presentation of enteric infections are discussed in this article. these include bacterial, viral, and parasitic illnesses, and are associated with the increasing popularity of drinking unpasteurized milk; eating raw fish and shellfish; consuming undercooked pork, poultry, and eggs; and having contact with pets. salmonella, campylobacter jejuni, vibrio, yersinia, aeromonas, edwardsiella, hepatitis a virus, norwalk virus, anisakis, eustrongylides, diphyllobothrium, na ...19911955706
[pansinusitis, brain abscess and subdural empyema caused by an unusual microorganism: edwardsiella tarda]. 19901969672
penetration and replication of edwardsiella spp. in hep-2 cells.the ability of 22 edwardsiella strains to penetrate and replicate in cultured epithelial cells was initially evaluated by light microscopy methods and by the recovery of gentamicin-resistant (gmr) bacteria from the triton x-100 cell lysates of hep-2-infected monolayers. giemsa-stained hep-2 cells revealed the presence of numerous internalized bacteria 3 h postinfection, often appearing as parallel rows of replicated bacteria within the cytosol and sometimes obliterating the cytoplasm because of ...19911987028
fish mortality in the mississippi catfish farming industry in 1988: causes and treatments.the 1988 fish mortality summary for the catfish (ictalurus punctatus) industry in mississippi is presented. in 1988, 2,456 cases were submitted to mississippi cooperative extension service fish disease laboratories at belzoni and stoneville. bacterial infection caused by edwardsiella ictaluri was the leading cause of catfish mortality. descriptions and treatments are presented for bacterial, parasitic, viral and other diseases affecting mississippi farm-raised catfish in 1988.19912023314
in vitro responses of channel catfish, ictalurus punctatus, neutrophils to edwardsiella ictaluri.the in vitro responses of channel catfish peripheral blood neutrophils to edwardsiella ictaluri were examined through the use of phagocytic, bactericidal, and chemiluminescent assays. evidence from both light and electron microscopy indicate that catfish neutrophils appeared to phagocytose e. ictaluri. although extracellular killing of e. ictaluri was observed, bactericidal assays did not demonstrate intracellular killing of e. ictaluri by neutrophils. catfish neutrophils mount a chemiluminescen ...19912050246
[reactions of different enterobacteriaceae and vibrionaceae species in brila-mug (fluorocult) bouillion].in order to test the usefulness of brila-mug (= fluorocult) medium (merck) for isolation and identification of total coliforms and faecal coliforms in surface water according to the ec guidelines for bathing waters a total of 969 strains of different enterobacteriaceae and vibrionaceae species was examined under different culture conditions. these included 486 e. coli (reference strains of o-groups 1-170, enterotoxin and verotoxin-producing strains), 149 salmonella (subspecies i-vi), 92 yersinia ...19902080970
["edwardsiella tarda" in marine animals]. 19902131605
production of shiga-like toxin among escherichia coli strains and other bacteria isolated from diarrhea in são paulo, elevated level of shiga-like toxin i (slt-i) production was found in 1 of 466 escherichia coli strains studied. among the 34 sonic lysates obtained from classical enteropathogenic e. coli, 5 produced slt-i. the aeromonas, citrobacter, edwardsiella, enterobacter, klebsiella, proteus, providencia, pseudomonas, salmonella, serratia, shigella, yersinia, and vibrio strains also studied were not slt producers, except for a shigella dysenteriae type 1 strain. although slt-i-producing e. coli strains ...19902199511
[a case of sepsis caused by edwardsiella tarda complicated panophthalmitis and pyogenic spondylitis].edwardsiella tarda (e. tarda) is gram negative enterobacteriaceae which has been found generally in animal hosts and occasionally in human feces. we have reported a case of sepsis caused by e. tarda, complicated panophthalmitis and pyogenic spondylitis. a 39-year old patient suffered from fever, polyarthralgia and lumbago. we performed blood culture, from which e. tarda was isolated. spinal ct scan showed destruction and osteogenesis of the fourth and fifth lumbar vertebral body and cranial ct s ...19902212756
life-threatening edwardsiella tarda soft-tissue infection associated with catfish puncture wound. 19902230281
homology among bacterial catalase genes.catalase activities in crude extracts of exponential and stationary phase cultures of various bacteria were visualized following gel electrophoresis for comparison with the enzymes from escherichia coli. citrobacter freundii, edwardsiella tarda, enterobacter aerogenes, klebsiella pneumoniae, and salmonella typhimurium exhibited patterns of catalase activity similar to e. coli, including bifunctional hpi-like bands and a monofunctional hpii-like band. proteus mirabilis, erwinia carotovora, and se ...19902253114
evolution of aromatic amino acid biosynthesis and application to the fine-tuned phylogenetic positioning of enteric bacteria.a comprehensive phylogenetic tree for virtually the entire assemblage of enteric bacteria is presented. character states of aromatic amino acid biosynthesis are used as criteria, and the results are compared with partial trees based upon sequencing of 16s rrna, 5s rrna, and tryptophan leader peptide. three major clusters are apparent. enterocluster 1 possesses a gene fusion (trpg-trpd) encoding anthranilate synthase: anthranilate 5-phosphoribosylpyrophosphate phosphoribosyltransferase of tryptop ...19902298692
soft-tissue infection caused by edwardsiella tarda and aeromonas hydrophila. 19902319177
a medium for the selective isolation of edwardsiella ictaluri.a selective medium, called edwardsiella ictaluri medium (eim), has been formulated for the isolation of edwardsiella ictaluri. the medium inhibits the growth of most gram-negative bacteria, except proteus sp., serratia marcescens and some isolates of aeromonas hydrophila and yersinia ruckeri. the bacteria that grow on the eim are easily differentiated from e. ictaluri based on colony morphology. the eim inhibits gram-positive bacteria with the exception of enterococci. the addition of fungizone ...19902338726
a cluster of edwardsiella tarda infection in a day-care center in florida. 19902388007
plasmid homologies in edwardsiella ictaluri.plasmids from all available non-channel catfish isolates of edwardsiella ictaluri were classified by gel electrophoresis and hybridization methods. all isolates, regardless of source, contained classes of homologous plasmids with similar but not identical sizes.19892619312
automated biochemical identification of bacterial fish pathogens using the abbott quantum ii.the quantum ii, originally designed by abbott diagnostics for automated rapid identification of members of enterobacteriaceae, was adapted for the identification of bacterial fish pathogens. the instrument operates as a spectrophotometer at a wavelength of 492.600 nm. a sample cartridge containing 20 inoculated biochemical chambers is inserted in the path of the analyzing beam. reactions are converted into a 7-digit octal biocode, relayed via a sensor to the memory module, and compared to biocod ...19892644450
bacterial diseases of marine fish.the principal bacterial diseases found among wild and cultured marine fish are reviewed. the bacterial agents discussed include the gram-negative pathogens in the vibrio, aeromonas, pasteurella and edwardsiella genera, renibacterium salmoninarum and the myxobacteria, streptococci, mycobacteria, nocardias and anaerobic organisms which have been associated with fish diseases. methods for the isolation and identification of these organisms are described.19892678720
dietary influences on disease resistance factors in channel catfish.the effects of diet on selected disease resistance factors were studied in channel catfish (ictalurus punctatus). two commercial feeds and a "control," laboratory-prepared diet were compared. macrophage function (phagocytosis and intracellular killing) was used to assess nonspecific disease resistance and serum antibody was measured as an indication of specific immunity. we measured the immune response to edwardsiella ictaluri, a common bacterial pathogen of catfish, as well as the phagocytosis ...19892767308
surface properties and ultrastructure of edwardsiella species.twenty-two strains of edwardsiella representing the three currently recognized species were evaluated for surface characteristics and ultrastructural morphology. all isolates tested possessed a high surface cell charge as detected by deae-cellulose chromatography; surface hydrophobicity was variable and strain but not species dependent. two major types of adhesins detected by hemagglutination assays were identified: one was inhibited by d-mannose (mannose-sensitive hemagglutinin) and found on al ...19892768468
development and testing of invasion-associated dna probes for detection of shigella spp. and enteroinvasive escherichia coli.genetic determinants of the invasive phenotype of shigella spp. and enteroinvasive escherichia coli (eiec), two common agents of bacillary dysentery, are encoded on large (180- to 210 kilobase), nonconjugative plasmids. several plasmid-encoded antigens have been implicated as important bacterial ligands that mediate the attachment and invasion of colonic epithelial cells by the bacteria. selected invasion plasmid antigen (ipa) genes have recently been cloned from shigella flexneri serotype 5 int ...19882830310
[first isolation of edwardsiella tarda from fish in abidjan: a possible source of human contamination].the bacteriological analysis of 127 fish viscera effected for the research of enteropathogenic germs showed that 8% of them contain edwardsiella tarda and can be a possible source of human infections.19882841045
rapid plasmid analysis for identification of edwardsiella ictaluri from infected channel catfish (ictalurus punctatus).eighteen different strains of edwardsiella ictaluri isolated from infected channel catfish (ictalurus punctatus) were screened to determine whether plasmid dna was present. two plasmids of 5,700 and 4,900 base pairs were identified. restriction enzyme analysis showed that each of the strains harbored these same two plasmids. restriction maps of the separated plasmids indicated that these plasmids were not closely related to each other. a rapid screening technique was developed that would allow t ...19873038017
drug-resistant plasmids from fish pathogens.the epidemiological surveillance of drug-resistant strains of the fish pathogenic bacteria vibrio anguillarum and pasteurella piscicida carrying transferable r plasmids in fish farms is described. the dna structure of r plasmids, and the drug-resistant determinants of r plasmids from the fish pathogens aeromonas hydrophila,aeromonas salmonicida, edwardsiella tarda, v. anguillarum, and p. piscicida are discussed.19883079184
edwardsiella tarda septicemia complicating acute leukemia.we describe here an unusual case of edwardsiella tarda septicemia preceded by acute gastroenteritis in a patient with acute leukemia in complete remission receiving maintenance chemotherapy. she also had ulcerated rectal cancer with bleeding, which was thought to serve as the portal of entry into the blood stream. she was successfully treated with cefmetazole and gentamicin in combination. however, the concomitant resolution of chemotherapy-induced granulocytopenia may have had a favorable effec ...19883193663
inhibitory activity of antibiotic-producing marine bacteria against fish pathogens.the activity of antibiotic-producing marine bacteria was assayed against bacterial fish pathogens belonging to the genera vibrio, aeromonas, pasteurella, edwardsiella, yersinia and pseudomonas with the aim of evaluating the possible use of these marine strains for controlling epizootics in aquaculture. inhibition tests on solid medium showed that, in general, the majority of fish bacteria were strongly sensitive to the marine bacteria. only two strains (edwardsiella tarda and pseudomonas aerugin ...19883204073
chemical characterization of lipopolysaccharide from edwardsiella ictaluri, a fish pathogen.the chemical components of lipopolysaccharide (lps) from the fish pathogen edwardsiella ictaluri (ed. ictaluri) were analyzed by sds-page, gas chromatography, and spectrophotometry, and compared with those of salmonella typhimurium and escherichia coli 0111:b4. only four to five low molecular weight species of lps from ed. ictaluri were detected by silver staining after separation by polyacrylamide gel electrophoresis. the low molecular weight species, as well as a low sugar content, indicate th ...19883208199
neonatal sepsis and meningitis caused by edwardsiella tarda. 19883231505
edwardsiella tarda serotyping scheme for international use.a combination of two systems for the serotyping of edwardsiella tarda developed independently at the national institute of health, tokyo, japan, and the centers for disease control, atlanta, ga., has enabled a single serotyping scheme comprising 61 o groups and 45 h antigens to be established for international use.19883235660
isolation, characterization, and molecular cloning of cryptic plasmids isolated from edwardsiella ictaluri.fifty-five isolates of edwardsiella ictaluri were examined for the presence of plasmid dna by a rapid alkaline extraction procedure. all 49 isolates from channel catfish and a single isolate from bengal danio carried 2 plasmids with molecular masses of approximately 3.2 and 3.7 megadaltons (mdal). five e ictaluri isolates from other fish contained 1 to 3 plasmids, which had molecular masses ranging from 2.5 to 45 mdal. the 2 plasmids (3.2 and 3.7 mdal) from the type strain of e ictaluri (atcc 33 ...19883247906
effect of recent antibacterial agents against bacteria causing diarrhoea.the susceptibility of 424 bacterial isolates causing diarrhoea were tested by agar dilution technique on mueller-hinton agar against amoxicillin, ampicillin, ceftriaxone, chloramphenicol, co-trimoxazole, ciprofloxacin, doxycycline, norfloxacin and ofloxacin. the bacterial species included were aeromonas hydrophila, edwardsiella tarda, pleisomonas shigelloides, salmonella spp., shigella spp., vibrio cholerae and vibrio parahaemolyticus. the most active compounds were the fluorinated 4-quinolones ...19883266900
kell blood group activity of gram-negative understand better the relationships between blood-group antigens and bacterial constituents, examples of 23 gram-negative bacteria (representing the 10 genera citrobacter, edwardsiella, enterobacter, escherichia, klebsiella, proteus, pseudomonas, salmonella, serratia, and shigella) were tested for the presence of kl-like antigens by hemagglutination-inhibition (hai) assays against both igg and igm anti-kl. saline-suspended whole organisms, cell-free culture media, and disrupted organisms were ...19883368935
the phylogenetic origin of the bifunctional tyrosine-pathway protein in the enteric lineage of bacteria.because bifunctional enzymes are distinctive and highly conserved products of relatively infrequent gene-fusion events, they are particularly useful markers to identify clusters of organisms at different hierarchical levels of a phylogenetic tree. within the subdivision of gram-negative bacteria known as superfamily b, there are two distinctive types of tyrosine-pathway dehydrogenases: (1) a broad-specificity dehydrogenase (recently termed cyclohexadienyl dehydrogenase [cdh]) that can utilize ei ...19883386529
edwardsiella tarda bacteremia. 19873436323
[a strain of edwardsiella tarda isolated from a patient with chronic diarrhea]. 19873621316
class d tetracycline resistance determinants of r plasmids from the fish pathogens aeromonas hydrophila, edwardsiella tarda, and pasteurella piscicida.tetracycline resistance determinants of r plasmids from the fish pathogens aeromonas hydrophila, edwardsiella tarda, and pasteurella piscicida were classified as class d by their expression of resistance to tetracycline and minocycline and through their dna structure.19873631946
effect of water temperature on the clinical outcome of infection with edwardsiella ictaluri in channel catfish fingerlings (mean body weight, 19 +/- 3 g each) were given intraperitoneal inoculations of edwardsiella ictaluri suspensions of 10(4), 10(5), or 10(6) bacteria. control fish were inoculated intraperitoneally with sterile 0.85% nacl solution. ten-day trials were conducted at water temperatures of 17, 21, 23, 25, 28, and 32 c. differences in mortality between e ictaluri-infected fish and fish used as controls were observed at water temperatures of 23, 25, and 28 c, but not at tempe ...19873692982
antimicrobial susceptibility of edwardsiella ictaluri.edwardsiella ictaluri isolates collected over the past 7 yr from outbreaks of disease in fish occurring in different geographic areas were screened against 37 antimicrobial agents. edwardsiella ictaluri were found to be susceptible to most agents active against gram negative bacteria such as aminoglycosides, cephalosporins, the "newer" penicillins, quinolones, tetracyclines, chloramphenicol, nitrofurantoin, and potentiated sulfonamides. resistance was observed against colistin, sulfonamides, and ...19863712643
salmonellae and other enteropathogenic bacteria in the intestines of wall geckos in nigeria.the aerobic bacterial flora of the intestine of 150 wall geckos (hemidactylus brookei) was investigated. a variety of bacteria was recovered including 35 isolates of salmonella and several other species of enterobacteriaceae, viz. shigella sonnei - 2, edwardsiella tarda - 4, enterobacter spp - 8, citrobacter freudii - 3, serratia marcescens - 3, proteus spp - 35, klebsiella pneumoniae - 13, and escherichia coli - 17, isolates. eight salmonella serotypes were identified, the predominant ones bein ...19863729372
modulary effects of temperature on antibody response and specific resistance to challenge of channel catfish, ictalurus punctatus, immunized against edwardsiella ictaluri.the effects of various temperature treatments on the level of the humoral antibody response in channel catfish immunized with formalin killed edwardsiella ictaluri was determined in laboratory controlled experiments. immunized fish that were held at 25 degrees c for 30 days and 12 degrees c for an additional 30 days had higher antibody titers, and were more protected upon challenge, than immunized fish held at 25 degrees c for 60 days. also immunized catfish held at 25 degrees c for 5 or 10 days ...19863765350
antimicrobial susceptibility of edwardsiella tarda from the united states and taiwan.edwardsiella tarda isolates from the united states and taiwan were screened against 37 antimicrobial agents. these isolates were highly susceptible to the amino-glycosides, second and third generation cephalosporins, newer penicillins, nitrofurantoin, sulfamethoxazole/trimethoprim, and the quinolones. source-related resistance was found for penicillin g, sulfadiazine, colistin, novobiocin, spectinomycin, ampicillin, tetracycline, and chloramphenicol. generally, the taiwan isolates were more resi ...19863776095
rapid catalase supplemental test for identification of members of the family enterobacteriaceae.a simple, rapid, semiquantitative slide catalase test useful for differentiating members of the family enterobacteriaceae is described. judging by the time required for appearance of oxygen bubbles in 3% hydrogen peroxide, the immediate catalase reactors were yersinia, serratia, proteus, morganella, providencia, cedecea, and hafnia spp. the delayed catalase reactors were escherichia, shigella, klebsiella, enterobacter, salmonella, citrobacter, edwardsiella, kluyvera, and tatumella spp. this info ...19873818934
edwardsiella tarda infection in hat yai hospital. 19863819621
exotic bacterial pathogens edwardsiella tarda and edwardsiella ictaluri from imported ornamental fish betta splendens and puntius conchonius, respectively: isolation and quarantine significance. 19863827773
disease patterns in the detroit zoo: a study of reptilian and amphibian populations from 1973 through 1983.a retrospective study was conducted to determine disease patterns in reptilian and amphibian populations at the detroit zoo from 1973 through 1983. in the reptilian population (mean +/- sd = 285.2 +/- 28), overall annual mortality rates were 1% to 40%. mortality rates were highest in the fall months (20%) and lowest in the winter months (6%). the most frequently affected reptiles were iguana (iguana iguana), reticulated python (python reticulatus), rattlesnakes (crotalus spp), common boa (constr ...19853878352
liver abscess caused by edwardsiella tarda.edwardsiella tarda, among other enterobacteriaceae, is a rare isolate from clinical specimens in man. a liver abscess caused by e. tarda was found in a middleaged woman without overt epidemiologic exposure to known reservoirs of this organism. while the most likely source of infection was the bowel, we failed to demonstrate a gut carriage of e. tarda or to show any local condition predisposing to the development of the liver lesion.19853911993
biochemical characteristics of edwardsiella ictaluri.a total of 119 isolates of edwardsiella ictaluri collected over the last 7 years from several states were biochemically characterized. although not very reactive biochemically, this bacterium shows a high degree of homogeneity. differences were found only in the production of gas from formate or from glucose at 37 degrees c and in the production of hydrogen sulfide as detected by lead acetate paper. analysis of e. ictaluri by year of geographic area indicated that some differences existed, but n ...19863954336
rapid microbiochemical method for presumptive identification of gastroenteritis-associated members of the family enterobacteriaceae.a method for rapid screening of isolates of pathogenic members of the family enterobacteriaceae is described. flow charts are used in conjunction with triple sugar iron agar, o-nitrophenyl-beta-d-galactopyranoside-phenylalanine-motility sulfate screening media, oxidase test, and six rapid biochemical tests, namely, lysine decarboxylase, urease, indole, esculin hydrolysis, malonate, and xylose. this scheme is used to provide an inexpensive but rapid presumptive identification of salmonella, shige ...19854008622
chemiluminescence by peripheral blood phagocytes from channel catfish: function of opsonin and temperature.the ability of the peripheral blood phagocytes from channel catfish, ictalurus punctatus, to produce chemiluminescence (cl) following ingestion of the enteric pathogen edwardsiella ictaluri was evaluated under a variety of opsonic and temperature conditions. the cl response was influenced by the concentration of immune serum used to opsonize the bacteria as well as the presence of nonspecific, heat labile opsonins. peak light emissions were diminished and the time to peak response was increased ...19854018342
comparative in vitro activities of selected antimicrobial agents against edwardsiella tarda.mics of 14 antimicrobial agents for 29 strains of edwardsiella tarda were determined by an agar dilution method. of the agents tested, ciprofloxacin, enoxacin, and norfloxacin were the most active on a weight basis. all strains were also susceptible to clinically achievable concentrations of ampicillin, chloramphenicol, tetracycline, trimethoprim, sulfamethoxazole, trimethoprim plus sulfamethoxazole, cefotaxime, and gentamicin. ninety percent of the strains demonstrated high-level resistance to ...19854026271
chronic enteritis associated with edwardsiella tarda infection in rockhopper penguins. 19854077643
a preliminary study on the intestinal flora of cecum and colon of eight, ten and 12 week old swine.using a replica-plating method and differential culture media, members of the enterobacteriaceae and pseudomonaceae were determined quantitatively in the colon and cecum content of pigs from different litters, born on the same farm and kept under the same environmental conditions, from the age of six weeks. the bacterial flora in pigs of eight, ten and 12 weeks of age showed little significant quantitative variation. pigs of 12 weeks of age differed significantly from younger ones with respect t ...19734270808
edwardsiella tarda in jungle diarrhoea and a possible association with entamoeba histolytica. 19714332825
sickle hemoglobinopathy and edwardsiella tarda meningitis. 19744415769
selective medium for hydrogen sulfide production by salmonellae.triple sugar iron agar does not reveal hydrogen sulfide production by all salmonella organisms nor does it permit clear-cut separation of those nonsalmonellae which produce h(2)s. numerous media with varied combinations of nutrients, inhibitors, selective agents, ph levels, and metal salts were tested for h(2)s production of cultures of salmonella, citrobacter, edwardsiella, arizona, proteus, providencia, klebsiella, and enterobacter. an agar medium has been devised which promotes growth and h(2 ...19724557561
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