Publications

TitleAbstractYear
Filter
PMID
Filter
effect of heat-killed cells of achromobacter nematophilus poinar et thomas, and the fraction (endotoxin) isolated from them on galleria mellonella l. caterpillars. 19751192248
proceedings: effects of histamine, 2-methylhistamine and 4-methylhistamine on blood pressure and vascular resistance in the cat. 19751151865
effect of glucose and insulin on protein and basement membrane synthesis in isolated glomeruli of diabetic rats.in incubation experiments with isolated glomeruli, an increased synthesis of protein and basement membranes was detected in diabetic rats compared to metabolically healthy controls. different glucose concentrations in the incubation medium and insulin did not influence protein and basement membrane synthesis of nondiabetic glomeruli. on the other hand, in diabetic glomeruli the synthetic activity depends on glucose concentration. insulin had a stimulating effect on protein and basement membrane ...1979525208
[effectiveness of fibrinolytic agents in complex treatment of traumatic endophthalmitis]. 19751082561
[effusions in the dog. 1. clinico-diagnostic study with special reference to the cytology of the fluid]. 19751231052
the lecithin: cholesterol acyltransferase reaction, lysolecithin and red cell ageing in blood stored under normal transfusion service conditions.the lecithin:cholesterol acyltransferase (lcat) activity and lysolecithin content of human blood stored under standard blood transfusion service conditions at 4 degrees c for 6 weeks has been investigated. cooling the blood to 4 degrees c rapidly inactivates the lcat reaction, but the enzyme is not denatured during storage under these conditions. citrate in the anticoagulant did not activate the lcat reaction in freshly-taken whole blood. the total phospholipid and total lysolecithin content of ...19761251568
surveillance of pertussis in the cssr. iv. immunological surveys of antibodies to pertussis and parapertussis in the bohemian regions and in slovakia in 1958 - 1971. 1976184206
juvenile periodontitis (periodontosis).juvenile periodontitis, characterized by early age of onset, rapid rate of periodontal tissue destruction and selective tooth involvement in the absence of commensurate aetiological factors, is recognized as a distinct clinical entity. the present paper describes the clinical findings and some immunological characteristics in a group of thirty-four patients under the age of thirty. clinical aspects studied included the influence of sex, race, family history and general health. immunological find ...1977267621
location of adult and fetal aldolases a, b, and c by immunoperoxidase technique in lf fast-growing rat hepatomas.the resurgence of aldolase isozymes in cancerous tissues is a well-known but poorly understood phenomenon. this resurgence poses the problem of whether or not adult and fetal aldolase isozymes are produced by the same cells. for clarification of this question, the immunoperoxidase technique was used to locate aldolases a, b, and c in one type of fast-growing hepatoma, the lf hepatoma and, by comparison, in normal adult liver. under optical microscopy, aldolases a and c were located in the cytopl ...1978201371
changes in the plasma lipoprotein-apoproteins of guinea pigs in response to dietary cholesterol.the major apoproteins from four plasma lipoproteins were isolated from control and cholesterol-fed guinea pigs. apoproteins were studied by column chromatography, polyacrylamide gel electrophoresis, and amino acid analysis. dietary cholesterol altered the plasma apolipoproteins mainly by an enrichment in the content of arginine-rich polypeptide (arp) in all density fractions. this protein had a similar molecular weight (34 000), electrophoretic mobility, amino acid composition, and microheteroge ...1977201278
evaluation of the role of c4 in the cellular immune response in vitro. 1979156225
repair of deaminated cytosine residues of dna: biological significance of the absence of uracil from dna. 1978100113
behavioral measurement measurements of excitability changes in reward sites in the dog: a frequency dependent effect. 197899757
ultrastructural observations on the maturation and secretion of granules in atrial myocardium. 197899521
cytogenetic analysis of double satellites on marker chromosome no. 14. 197995131
[histochemical studies on the structure and the age changes of descemet's membrane in cattle (author's transl)].descemet's membrane of 15 cattle-corneas, aged 6 month to 13 years was studied by various histochemical and morphometric methods. 3 zones could be differentiated. zone 1 is relatively small and does not show significant changes with age; zone 2 comprises the main part of descemet's membrane, its diameter increased with age. zone 2 is rich in sulfated glycosaminoglycans. zone 3, below the endothelial lining, does not develop before the 3rd and 5th year of life and stains different from zone 2.197994246
anomalous alpha 2-macroglobulin-protease complexes in cystic fibrosis: decreased uptake of the complexes by fibroblasts in culture.immunochemical and functional properties of control and cystic fibrosis (cf) alpha 2-macroglobulin (alpha 2m) are compared. crossed immunoelectrophoresis and ouchterlony double diffusion revealed no qualitative differences between the two alpha 2-m preparations. trypsin-esterase activity assayed with bapna as a substrate, in the presence of an excess sti, gave similar ratios between total and active alpha 2m. these alpha 2m-trypsin complexes were equally stable under various experimental conditi ...197993265
from the nichd. national institute of child health and human development. predictors of hereditary disease or congenital defects. 197993264
penicillin and cephalosporin immunogenicity in man.whether cross-allergenicity exists between the penicillin and cephalosporin antibiotics is controversial. a solid-phase radioimmunoassay was adapted to detect drug-specific igg, igm and ige antibodies. penicillin and cephalothin-specific serum immunoglobulins were determined in patients' serum before and after a therapeutic course of sodium cephalothin. changes in igg, igm and ige antipenicillin and anticephalothin antibodies, respectively, occurred and were closely correlated to one another (r ...197992902
[physicochemical properties of neurospecific basic protein (scp) of the spinal cord].basic protein (scp) known as antiencephalytogenic was isolated from the bull spinal cord and spinal roots. the protein is purified to a molecular homogeneous state, some of its physicochemical properties are studied. molecular homogeneity of the protein is established by the method of disk electrophoresis in 15% polyacrylamide gel with sodium dodecyl sulphate present and by analytic ultracentrifugation. during electrophoresis in 15% polyacrylamide gel at ph 4.0 the protein movement occurs only i ...197992837
ia determinants on human t-cell subsets defined by monoclonal antibody. activation stimuli required for expression.the nature of ia antigens which appear on human t cells after activation and the stimuli required for their expression was examined utilizing a monoclonal antibody reactive with the ia antigen framework. t cells were purified using monoclonal antibodies directed either at the entire t-cell population (okt3) or the t-cell inducer subset (okt4). by indirect immunofluorescence, it was shown that the human t-cell population contains no detectable ia+ cells in the resting state. in contrast, in exces ...197992523
aryl hydrocarbon hydroxylase induction in mammalian liver-derived cell cultures. effects of various metabolic inhibitors on the enzyme activity in hepatoma cells. 1976181006
the influence of sodium, potassium and lanthanum on amino acid release from spinal-medullary synaptosomes. 1975166144
correspondence: growth plate defects in foals. 19761265980
lysogeny and bacteriocinogeny in xenorhabdus nematophilus and other xenorhabdus spp.induction by mitomycin or high-temperature treatment resulted in the production of bacteriocins and phages in both phases of xenorhabdus nematophilus a24, indicating lysogeny. phage dna purified from x. nematophilus a24 hybridized to several fragments of drai-digested a24 chromosomal dna, confirming that the phage genome was incorporated into the bacterial chromosome. bacteriocins and phages were detected in cultures of most other xenorhabdus spp. after mitomycin or high-temperature treatment. x ...19921444417
dna adduct measurements and tumor incidence during chronic carcinogen exposure in animal models: implications for dna adduct-based human cancer risk assessment. 19921489923
[fever, edema, eosinophilia and acute pneumonia: bilateral, diffuse pneumocystis carinii pneumonia and candida pneumonia of the left lung (evaluation of eosinophilia)]. 19751082519
pulsatile growth hormone secretion during the breeding season in male reindeer and its association with hypophagia and weight loss.male deer reduce food intake and lose weight during the breeding season (rut) in autumn. growth hormone (gh) is lipolytic in ruminants; therefore, pulsatile gh secretion was measured in three young reindeer (rangifer tarandus tarandus) bulls during the natural period of weight loss during rut. blood samples were withdrawn through a jugular cannula every 20 min for 16 hr on 5 occasions from late summer to early winter in unanaesthetised animals. the deer were group fed a pelleted concentrate rati ...19921563616
death due to disruption of sternal fixation wire--a rare complication after open heart surgery through sternotomy: report of a case.following sternotomy for open heart surgery, a 57-year-old man had the sternum closed in the usual manner with six no.22 gauge stainless steel wire sutures. twenty-four days later, sternum disruption occurred with only one wire suture in place, 3 wire sutures cutting through the bone, and 2 wire sutures fractured. a lacerating injury over the anterior surface of the right ventricle was rendered by these 2 fractured wires followed by fatal outcome. to our knowledge, death induced by fractured wir ...19892794943
syphilis and human immunodeficiency virus infection. 19921615239
a preliminary trial of beta-carotene in subjects infected with the human immunodeficiency virus.beta-carotene is a nontoxic carotenoid with immunomodulating properties in animals and humans. based on our observations in normal immunocompetent subjects, we studied the effects of this compound in 11 patients infected with the human immunodeficiency virus (hiv). each subject received 60 mg of beta-carotene daily for 4 mo. clinical and laboratory studies were obtained at baseline, every month while on treatment and for 2 mo after treatment. increases in the percent of cells expressing leu 11 ( ...19921347316
the role of monocyte/macrophages and cytokines in the pathogenesis of hiv infection.the monocyte/macrophage system, which is characterized by a high degree of heterogeneity, is a target of the human immunodeficiency virus (hiv) in vitro as well as in vivo. both bone-marrow-derived precursor elements and circulating monocytes are infectable in vitro and have also been found infected in seropositive individuals. however, terminally differentiated macrophages are the most commonly infected cells found in vivo in addition to the cd4+ t lymphocytes. several immunoregulatory cytokine ...19921388722
antimutagenic and anticarcinogenic effects of betel leaf extract against the tobacco-specific nitrosamine 4-(n-nitrosomethylamino)-1-(3-pyridyl)-1-butanone (nnk).earlier studies showed that betel leaf inhibits the mutagenic action of standard mutagens like benzo[a]pyrene and dimethylbenz[a]anthracene. since tobacco-specific nitrosamines are the major carcinogens present in unburnt forms of tobacco, we studied the effect of an extract of betel leaf on the mutagenic and carcinogenic actions of one of the most potent, 4-(n-nitrosomethylamino)-1-(3-pyridyl)-1-butanone (nnk). betel-leaf extract and hydroxychavicol suppressed the mutagenicity of nnk in both th ...19911855909
acute effects of intravenous propafenone on the internal ventricular defibrillation threshold in the anesthetized dog.in 10 treated and 2 control dogs, the short-term effects of intravenous propafenone (2 mg/kg/10 minutes, followed by 1 mg/min [n = 2] or 25 micrograms/kg/min [n = 8]) on the internal ventricular defibrillation energy requirements (der) were investigated. multiple stored energy levels were randomly tested and the percent successful defibrillation was plotted against the stored energy, and the raw data were fit by logistic regression. the energy at 50% (e50) and 80% (e80) defibrillation success in ...19911951000
[resonance stimulation of regional ventilation of the lungs by sound vibration in the prevention of acute respiratory insufficiency]. 19892704716
histochemistry of tissue reaction in the heart of cattle experimentally infected with cysticercus bovis. 1979161543
plasmids and phase variation in xenorhabdus spp.three strains of xenorhabdus nematophilus (a24, f1, nc116) and strain dan of xenorhabdus bovienii were tested to evaluate whether the phase variation observed in these bacteria was in any way connected with plasmids. the plasmid patterns of both phases of a24 and f1 strains were the same, whereas the two nc116 phases had only one band each. no difference was observed between the undigested or digested plasmid patterns of the two phases from the three strains. no plasmid was detected in either ph ...19911768133
treatment of acute migraine with subcutaneous sumatriptan.sumatriptan succinate, a 5-ht1d receptor agonist, constricts human cranial arteries. two parallel-group trials for treatment of acute migraines were conducted in the united states. adult patients were randomized and given either 6 mg of sumatriptan succinate subcutaneously (n = 734) or placebo (n = 370). at 1 hour, sumatriptan was significantly more effective than placebo in reducing moderate or severe headache pain to mild or no pain (70% vs 22%), in completely relieving headaches (49% vs 9%), ...19911851894
bullous pemphigoid antigen: cdna cloning, cellular expression, and evidence for polymorphism of the human gene.a human epidermal keratinocyte lambda gt11 recombinant cdna library was screened with a 0.45-kb cdna that was generated by polymerase chain reaction (pcr) amplification of a segment of human bullous pemphigoid (bp) antigen mrna. the screen yielded five clones, the largest one, pcbpa-4, being 2.3 kb in size. the pcbpa-4 cdna hybridized in northern analyses with an approximately 9-kb mrna from cultured keratinocytes, whereas no hybridization signal was detected with rna from human skin fibroblast, ...19912045679
antagonizing effects of phorbol 12-myristate 13-acetate on hormonally stimulated gluconeogenesis in isolated rat hepatocytes involve activity changes of pyruvate kinase.the tumor-promoting phorbol ester phorbol 12-myristate 13-acetate partially neutralized the stimulatory effects of epinephrine (alpha 1-adrenergic actions), glucagon, and dibutyryl-camp on gluconeogenesis in isolated hepatocytes of fasted rats, when lactate or dihydroxyacetone was used as the substrate. by constructing metabolic crossover plots and by comparing rates of lactate production from dihydroxyacetone with k0.5 values of extracted pyruvate kinase for phosphoenolpyruvate, we obtained evi ...19902168154
dopamine denervation of the nucleus accumbens induces a selective increase in the number of delta-opioid binding sites.selective lesions of dopamine (da) neurons of the nucleus accumbens were made in rats by local injections of 6-hydroxydopamine (6-ohda). seven days after 6-ohda, the binding affinities and capacities at mu-, delta- and kappa-opioid binding sites were determined in the nucleus accumbens by selective labelling techniques. 6-ohda pretreatment caused a significant increase in the number of delta-opioid binding sites (+30%) while the number of mu-, kappa- and total sites was not modified. the affinit ...19872825926
dopamine denervation of the nucleus accumbens induces a selective increase in the number of delta-opioid binding sites.selective lesions of dopamine (da) neurons of the nucleus accumbens were made in rats by local injections of 6-hydroxydopamine (6-ohda). seven days after 6-ohda, the binding affinities and capacities at mu-, delta- and kappa-opioid binding sites were determined in the nucleus accumbens by selective labelling techniques. 6-ohda pretreatment caused a significant increase in the number of delta-opioid binding sites (+30%) while the number of mu-, kappa- and total sites was not modified. the affinit ...19872825926
galanin-immunoreactive neurons in the guinea-pig small intestine: their projections and relationships to other enteric neurons.galanin immunoreactivity was observed in nerve cell bodies and nerve fibres, but not in enteroendocrine cells, in the small intestine of the guinea-pig. nerve terminals were found in the myenteric plexus, in the circular muscle, in submucous ganglia, around submucous arterioles, and in the mucosa. lesion studies showed that all terminals were intrinsic to the intestine; those in myenteric ganglia arose from cell bodies in more orally placed ganglia. myenteric nerve cells were also the source of ...19872446770
rapid staining of campylobacter pyloridis. 19872447004
post-implantation development and cytogenetic analysis of diandric heterozygous diploid mouse embryos.diandric heterozygous diploid mouse embryos were produced by standard micromanipulatory techniques using eggs from female mice with a normal chromosome constitution and fertilised by homozygous rb(1.3)1bnr males containing a pair of large metacentric marker chromosomes in their karyotype. the constructed diandric eggs were transferred to the oviducts of pseudopregnant recipients and subsequently autopsied midday on the eighth day of gestation. from a total of 85 eggs transferred to females that ...19892612210
post-implantation development and cytogenetic analysis of diandric heterozygous diploid mouse embryos.diandric heterozygous diploid mouse embryos were produced by standard micromanipulatory techniques using eggs from female mice with a normal chromosome constitution and fertilised by homozygous rb(1.3)1bnr males containing a pair of large metacentric marker chromosomes in their karyotype. the constructed diandric eggs were transferred to the oviducts of pseudopregnant recipients and subsequently autopsied midday on the eighth day of gestation. from a total of 85 eggs transferred to females that ...19892612210
activation of calpain i and calpain ii: a comparative study using terbium as a fluorescent probe for calcium-binding sites.the present study demonstrates the activation of calpain i and calpain ii by micromolar levels of terbium and has utilized the enhancement in the fluorescence of protein-bound terbium to study and compare the calcium binding sites of the two enzymes. calpain i and calpain ii were isolated from bovine erythrocytes and brain, respectively. while the rates of activation of calpain i by terbium and calcium are comparable, the rate of activation of calpain ii was much greater in the presence of terbi ...19882847653
sensitivity and specificity of an enzyme-linked immunosorbent assay using the recombinant dna-derived treponema pallidum protein tmpa for serodiagnosis of syphilis and the potential use of tmpa for assessing the effect of antibiotic therapy.the recombinant dna-derived treponema pallidum membrane protein tmpa, purified from escherichia coli k-12, was used in an enzyme-linked immunosorbent assay (elisa) to evaluate its suitability in a screening test for syphilis and to monitor the effect of antibiotic treatment. the sensitivity of the tmpa elisa was 76% for primary syphilis, 100% for secondary syphilis, and 98% for early latent syphilis. all except 1 of 15 serum samples positive for yaws were positive in this test. a specificity of ...19892643617
sensitivity and specificity of an enzyme-linked immunosorbent assay using the recombinant dna-derived treponema pallidum protein tmpa for serodiagnosis of syphilis and the potential use of tmpa for assessing the effect of antibiotic therapy.the recombinant dna-derived treponema pallidum membrane protein tmpa, purified from escherichia coli k-12, was used in an enzyme-linked immunosorbent assay (elisa) to evaluate its suitability in a screening test for syphilis and to monitor the effect of antibiotic treatment. the sensitivity of the tmpa elisa was 76% for primary syphilis, 100% for secondary syphilis, and 98% for early latent syphilis. all except 1 of 15 serum samples positive for yaws were positive in this test. a specificity of ...19892643617
dose and time-course evaluation of a redox-based estradiol-chemical delivery system for the brain. ii. pharmacodynamic responses.clinically, brain-enhanced delivery and sustained release of estradiol (e2) are desirable for effective treatments of menopausal hot flushes and prostatic adenocarcinoma and for fertility regulation. thus, we conducted studies to determine the dose- and time-dependent effects of a brain-enhanced estradiol-chemical delivery system (e2-cds) on anterior pituitary hormones secretion in ovariectomized (ovx) rats. the e2-cds has consistently demonstrated preferential retention of its intermediate meta ...19902293207
studies on the osmophilic fungus wallemia sebi as an allergen evaluated by skin prick test and radioallergosorbent test.recently, wallemia sebi, a species of osmophilic fungi, has been abundantly detected in house dust using low water activity media. in this study, allergenic activity of w. sebi was assessed by skin prick test and radioallergosorbent test (rast) in 74 asthmatic patients ranging from 6 to 32 years of age. aspergillus fumigatus and house dust were used for comparison. in skin prick test, w. sebi extract, a. fumigatus extract and house dust extract elicited positive reactions in 4 (5.4%), 4 (5.4%) a ...19892613343
growth hormone-dependent insulin-like growth factor (igf) binding protein from human plasma differs from other human igf binding proteins.a growth hormone-dependent binding protein for insulin-like growth factors (igf-i and igf-ii) has been isolated from human plasma. analyzed on sds gels, the preparation contained a major protein band of 53 kda, and a minor band of 47 kda. after transfer to nitrocellulose, both species bound iodinated igf-i, and could be detected using an antibody raised against the purified preparation. in contrast, an igf binding protein purified from human amniotic fluid bound igf-i but was not detectable immu ...19862945561
[the prevention of atrophic rhinitis of swine].in a pig breeding unit that for years had been infected with atrophic rhinitis, the weaned piglets were divided into four groups. group 1 (10 weaned piglets) --their mother was vaccinated with 2 ml rhinipig i.m. 3 weeks prior to farrowing. --the piglets received 0.2 ml each of pargenta 50 (= 10 mg gentamycin) i.m. on the third, fifth and seventh days after birth. --the piglets were fed a food containing 10 ppm gentamycin-base during the 6 weeks following their weaning. group 2 (10 weaned piglets ...19892719633
[induction of granulomatous liver inflammation by noninfectious particles].a single intravenous injection of 0.1 mg/g of zymosan led to the formation of granuloma-type clusters of mononuclears in the liver of cba mice or (cba x c57b1)f1 hybrid mice. the area occupied by the granulomas grows for 3 to 9 days and then gradually diminishes so that the granulomas disappear practically in one month. hydrocortisone injected in a dose of 125 mg/kg 2 and 24 hours before or 2 and 24 hours after zymosan inhibited the initiation of granulomas but, at the same time, led to their sl ...19902293164
culture of islets of langerhans from an infant with intractable neonatal hypoglycemia: cytochemical and radioimmunological studies.extralobular islet of langerhans cells were isolated from the pancreas of an infant with intractable neonatal hypoglycemia (nesidioblastosis) by digestion with a mixture of trypsin and collagenase, and subsequent purification on a gradient of fetal calf serum. these islet cells cultured in eagle's medium containing 20% fetal calf serum formed confluent endocrine cell monolayers within 15 days. these endocrine cells were studied immunocytochemically, and their secretion products were assayed by r ...19892698367
[comparison of the frequency and intensity of vibrations of dental drive systems in tooth preparations ].during high speed cavity preparation the vibration burden of human teeth was evaluated in comparison between turbine- and micromotor-driven handpieces. the acceleration spectra of different burs showed an advantage of the air-driven handpiece.19892698343
transformation of xenorhabdus nematophilus.the ability of xenorhabdus nematophilus 19061/1 to be transformed by phk17 plasmid dna was studied and optimized. a number of factors, including culture conditions, stage of growth, transformation buffer ph, cation type and concentration required for the production of competency, washing, heat shock conditions, and cell-dna ratio, were found to affect transformation significantly. on the basis of these observations, a procedure for the routine transformation of x. nematophilus 19061/1 at frequen ...19892729982
[trans-sigmoid urinary diversion and dediversion and cancer: experimental study].the present experimental study was carried out in 75 female wistar rats. end-to-side vesicosigmoidostomy was performed in 65; the remaining 10 rats comprised the control group. thirty-three rats were de-diverted at 1 and 2 months later. the site of the vesicosigmoid anastomosis was assessed histologically at 1, 2, and 3 months postoperatively in diverted rats, and at 1 and 2 months in de-diverted rats. squamous metaplastic changes and variable degrees of transitional cell line neoplasm were obse ...19892735776
heparin and glutathione: physiological decondensing agents of human sperm nuclei.it has been proposed that reduced glutathione (gsh) or other thiol reagents may participate in the basic mechanism by which sperm-decondensing activity is accomplished. however, in vitro, these reagents seem to be inactive and require the presence of other chemicals, usually detergents. heparin binds specifically to the sperm membrane and provokes the decondensation of human sperm and the activation of dna transcription and synthesis. however, the concentrations at which these effects occur seem ...19892744703
the defect of lymphocyte locomotion in chronic lymphocytic leukaemia: studies of polarization and growth-dependent locomotion.this paper reports a study of the locomotor behaviour of the lymphocytes from 17 patients with chronic lymphocytic leukaemia (cll). the cells were studied both immediately after separation from blood and after culture for 24 to 48 h with a range of growth activators. cells direct from blood were tested for polarization in fetal calf serum (fcs 20%), phorbol myristate acetate (pma 10(-7)m) and colchicine (10(-5)m). the polarization of lymphocytes from cll patients with high white cell (wbc) count ...19883383451
britain launches genome program. 19892928800
[changes in the cyclic nucleotide system of t-lymphocytes in experimental tuberculosis in animals]. 19882851783
xenorhabdus luminescens (dna hybridization group 5) from human clinical specimens.an unusual isolate from a human leg wound was identified as xenorhabdus luminescens. this finding led to the discovery or isolation of four additional strains, two from blood and two from wounds. three of the five strains were from patients in san antonio, tex. three strains were studied by dna-dna hybridization (s1 nuclease-trichloroacetic acid method) and were 77 to 100% related to each other, 34% related to the type strain of x. luminescens, 35 to 40% related to three of grimont's other dna h ...19892768446
detrimental actions of endogenous fatty acids and their derivatives. a study of ischaemic mitochondrial injury.functional and structural alterations of myocardial mitochondria were investigated after four conditions of myocardial ischaemia in guinea pig heart: (1) 45 min complete ischaemia, (2) 60 min low-flow anoxic perfusion (0.3 ml/g wet weight per minute) with a modified tyrode solution, (3) as (2) with 0.4 mm palmitic acid added to the perfusate, and (4) as (2) with 0.4 mm oleic acid added. under conditions (1) and (2) the loss of tissue atp (20-30% of aerobic control) and the degree of mitochondria ...19872821981
phosphorylation, glycosylation, and proteolytic activity of the 52-kd estrogen-induced protein secreted by mcf7 cells.we have studied the posttranslational modifications of the 52-kd protein, an estrogen-regulated autocrine mitogen secreted by several human breast cancer cells in culture (westley, b., and h. rochefort, 1980, cell, 20:353-362). the secreted 52-kd protein was found to be phosphorylated mostly (94%) on high-mannose n-linked oligosaccharide chains, and mannose-6-phosphate signals were identified. the phosphate signal was totally removed by alkaline phosphatase hydrolysis. the secreted 52-kd protein ...19873543022
the optimum time for neutering surgery of dogs and cats. 19883381459
the effects of anions on fluid reabsorption from the proximal convoluted tubule of the rat.1. fluid reabsorption from surface proximal tubules of the rat was measured in vivo using stationary microperfusion techniques. reabsorptive rate (jv) was measured from droplets containing chloride as the main reabsorbable anion and when chloride was substituted by bromide, iodide, nitrate, acetate, isethionate or methylsulphate in either the tubular lumen alone or in both lumen and peritubular capillaries. 2. in tubules with an intact blood supply, droplet volume decreased in a manner best desc ...19883256612
canine lymphoma: clinical considerations. 19863333887
current state and tendencies of antibiotic resistance in hungary.this survey is based on data for 245 903 isolates reported by public health network laboratories in 1983. facultatively pathogenic gram-negative bacteria comprised two-third of the isolates, and--except escherichia coli--were resistant in a high percent to the most frequently used antibiotics. oxacillin and vancomycin were the most effective against staphylococcus aureus being in 94.7% resistant to penicillin. in contrast to other streptococci, all streptococcus pyogenes strains were sensitive t ...19863811828
human term placenta releases placental protein 10 (pp10) in tissue culture.placenta, decidua, chorion and amnion were studied to determine the tissue concentration of placental protein 10 (pp10). as measured by radio-immunoassay, all tissues studied were found to contain approximately the same concentration of pp10, in both early and late pregnancy. the release of pp10 was studied in tissue culture, first by using explants of placenta, decidua, amnion and chorion in four preliminary studies. only the placenta released significant amounts of pp10 into the culture medium ...19863811843
canine lymphoma: clinical considerations. 19863333887
nonactivated and activated glucocorticoid receptor complexes from human salivary gland adenocarcinoma cell line.[3h]triamcinolone acetonide glucocorticoid receptor complexes from human salivary gland adenocarcinoma cells (hsg cells) were shown to be activated with an accompanying decrease in molecular weight in intact cells, as analyzed by gel filtration, deae chromatography, the mini-column method and glycerol gradient centrifugation. glucocorticoid receptor complexes consist of steroid-binding protein (or glucocorticoid receptor) and non-steroid-binding factors such as the heat-shock protein of molecula ...19883401512
quantitative carcinogenesis in relation to human exposures.while carcinogenic potency cannot be expressed in absolute terms, quantitative carcinogenesis nevertheless plays an important role both in assessing cancer risks and in devising effective methods of cancer prevention. attention is drawn to newer methods of prevention based on interference with the carcinogenic process, which is likely to counterbalance, or even to reverse, the anticipated trend of increased cancer incidence resulting from new carcinogens introduced in our environment.19883390839
paracentric inversions in man. a french collaborative study. groupe de cytogénéticiens français.the collection of paracentric inversions ascertained in french cytogenetics laboratories gives a sample of 32 unpublished cases. the carriers seem to have a normal reproductive fitness, without difference between males and females. no cases of aneusomie de recombinaison were found, and it is likely that almost all, and perhaps all cases were ascertained for a cause independent of the inversion. some chromosomes are too frequently affected: 3, 7 and 14; conversely chromosome 2 was never involved, ...19863491573
expression of the gene for bacillus subtilis aspartokinase ii in escherichia coli.the gene coding for the subunits of aspartokinase ii from bacillus subtilis has been identified in a b. subtilis dna library and cloned in a bacterial plasmid (bondaryk, r. p., and paulus, h. (1984) j. biol. chem. 259, 585-591). the introduction of a plasmid carrying the aspartokinase ii gene into an auxotrophic escherichia coli strain lacking all three aspartokinases restored its ability to grow in the absence of l-lysine, l-threonine, and l-methionine. the b. subtilis aspartokinase gene could ...19852981222
[development of polyribosomes during maturation of reticulocytes in vivo]. 19704098024
phosphorylation, glycosylation, and proteolytic activity of the 52-kd estrogen-induced protein secreted by mcf7 cells.we have studied the posttranslational modifications of the 52-kd protein, an estrogen-regulated autocrine mitogen secreted by several human breast cancer cells in culture (westley, b., and h. rochefort, 1980, cell, 20:353-362). the secreted 52-kd protein was found to be phosphorylated mostly (94%) on high-mannose n-linked oligosaccharide chains, and mannose-6-phosphate signals were identified. the phosphate signal was totally removed by alkaline phosphatase hydrolysis. the secreted 52-kd protein ...19873543022
localization and comparative nucleotide sequence analysis of the transforming domain in herpes simplex virus dna containing repetitive genetic elements.the 7.5-kilobase bamhi e fragment (bamhi-e) of herpes simplex virus type 2 (hsv-2) dna (map position 0.533-0.583) encodes the 144-kda subunit of ribonucleotide reductase and induces the neoplastic transformation of immortalized cell lines. to define the minimal transforming region of bamhi-e, a series of subclones were constructed that spanned the entire fragment. these subclones were assayed for focus formation in rat-2 cells. removal of the promoter region from the viral 144-kda-protein gene l ...19863020562
the chicken carbonic anhydrase ii gene: evidence for a recent shift in intron position.the complete nucleotide sequence of the coding region of the chicken carbonic anhydrase ii (ca ii) gene has been determined from clones isolated from a chicken genomic library. the sequence of a nearly full length chicken ca ii cdna clone has also been obtained. the gene is approximately 17 kilobase pairs (kb) in size and codes for a protein that is comprised of 259 amino acid residues. the 5' flanking region contains consensus sequences commonly associated with eucaryotic genes transcribed by r ...19873029691
pharmacokinetics of insulin following intravenous and subcutaneous administration in canines.studies were conducted to examine the absorption and disposition kinetics of insulin in dogs following intravenous (iv) and subcutaneous (sc) administration of commercial preparations. after iv and sc dosing, the plasma levels were described by models which considered basal insulin level contributions. intersubject variation in the disposition kinetics was small with half-lives of 0.52 +/- 0.05 h and total body clearances of 16.21 +/- 2.08 ml min-1 kg-1. calculated insulin plasma secretion rates ...19863535917
localization and comparative nucleotide sequence analysis of the transforming domain in herpes simplex virus dna containing repetitive genetic elements.the 7.5-kilobase bamhi e fragment (bamhi-e) of herpes simplex virus type 2 (hsv-2) dna (map position 0.533-0.583) encodes the 144-kda subunit of ribonucleotide reductase and induces the neoplastic transformation of immortalized cell lines. to define the minimal transforming region of bamhi-e, a series of subclones were constructed that spanned the entire fragment. these subclones were assayed for focus formation in rat-2 cells. removal of the promoter region from the viral 144-kda-protein gene l ...19863020562
differential transient and long-term expression of dna sequences introduced into t-lymphocyte lines.we have used a protoplast fusion protocol to introduce the genes encoding neomycin phosphotransferase (neo) and chloramphenicol acetyltransferase (cat) into murine and human t-lymphocyte lines. plasmid constructs containing the neo gene under the control of the promoters from the rous sarcoma virus long terminal repeat (rsv ltr), the sv40 early region, or the herpes simplex virus thymidine kinase gene (hsv tk) can stably transform each of three t-cell lines to g-418 resistance. the characteristi ...19863028736
kappa opioid receptor activation depresses excitatory synaptic input to rat locus coeruleus neurons in vitro.intracellular recordings were made from neurons of the rat locus coeruleus contained within a brain slice maintained in vitro. when applied to the slice in known concentrations the selective kappa opioid receptor agonist trans-(+)-3,4-dichloro-n-methyl-[2-(1-pyrrolidinyl)cyclohexyl] benzeneacetamide methane sulphonate (u50488) (0.01-1 microm) produced a concentration-dependent depression of the excitatory post-synaptic potential evoked by electrical stimulation of afferent inputs to the locus co ...19873031541
arachidonic acid inhibits phosphate transport by cultured renal cells.because arachidonic acid and its metabolites are reported to be intracellular messengers of various exogenous stimuli, we studied whether arachidonic acid influences phosphate transport by cultured mouse renal epithelial cells. arachidonic acid, at 10(-7)-10(-4)m, inhibited phosphate transport without influencing cyclic adenosine 3':5'-monophosphate production. nordihydroguaiaretic acid and indomethacin, inhibitors of arachidonic acid metabolism, did not cancel the arachidonic acid-induced inhib ...19873032193
arachidonic acid inhibits phosphate transport by cultured renal cells.because arachidonic acid and its metabolites are reported to be intracellular messengers of various exogenous stimuli, we studied whether arachidonic acid influences phosphate transport by cultured mouse renal epithelial cells. arachidonic acid, at 10(-7)-10(-4)m, inhibited phosphate transport without influencing cyclic adenosine 3':5'-monophosphate production. nordihydroguaiaretic acid and indomethacin, inhibitors of arachidonic acid metabolism, did not cancel the arachidonic acid-induced inhib ...19873032193
isolation of heart-resistant clostridium perfringens from healthy cattle. 19724333445
preservation of "peripheral" benzodiazepine receptors: differential effects of freezing on [3h]ro 5-4864 and [3h]pk 11195 binding.the equilibrium binding constants of [3h]ro 5-4864 (a "peripheral" benzodiazepine receptor ligand) to renal membranes preserved by various freezing techniques were investigated. the bmax for [3h]ro 5-4864 binding to membranes from kidneys preserved as unwashed homogenates stored at -80, -20, or 5 degrees c, whole kidneys stores at -20 or 5 degrees c or as washed homogenate stored at -20 degrees c was significantly decreased (approximately 35%). only when kidneys were frozen intact (using a dry-i ...19873035290
[electrophysiologic, histochemical and immunologic correlates in the central mechanism of audiogenic epilepsy]. 19724336346
[muscarinic receptors of the superior cervical lymph node of the cat]. 19664386667
mitomycin c-induced bidirectional transcription from the colicin e1 promoter region in plasmid cole1.treatment of a colicinogenic culture with mitomycin c induces convergent transcription from two adjacent promoters at the beginning of the colicin e1 gene. s1-mapping and primer extension assays indicate that the mitomycin c-inducible transcripts correspond to colicin e1 mrna (cea mrna) and to a transcript, designated rna-c, that may code for an entry exclusion function. nucleotide sequences that strongly resemble a consensus sequence for lexa protein binding sites span the transcription start p ...19863092859
effects of steroid and nonsteroid metabolites on enzyme conformation and pyridoxal phosphate binding. 19694391266
[studies on stimulation of immunological responses resulting from intravesical bcg instillation in patients with bladder carcinomas]. 19883251104
effect of heavy metals on the growth of tissue cultures (ii).the effect of toxic concentrations of three heavy metal compounds on the growth of the secondary callus tissue of nicotiana tabacum l. and ruta graveolens l. was studied. the metal compounds examined were znso4, niso4, cuso4. the metal compounds used were placed in murashige, skoog (1962) and white (1943) culture medium at 10(-6) and 10(-4) m concentration, respectively, before autoclaving. the culture media containing macro- and microelements and vitamins were completed with carbon source and r ...19883254011
effects of corn oil- and fish oil-supplemented diets on phospholipid fatty acid composition of rat liver nuclei.the effects of dietary fat on fatty acid compositions of phospholipids in liver nuclei were investigated. by feeding a diet containing 5% corn oil or fish oil to rats, the proportions of n -6 or n -3 polyunsaturated fatty acids (pufa), respectively, were increased in phospholipids of the liver nuclei. by feeding a fat-free diet, endogenous pufa were increased. even after feeding the fat-free diet for 40 weeks, the n - 6 still remained at about 10% in both phosphatidylcholine (pc) and phosphatidy ...19883196728
effect of heavy metals on the growth of tissue cultures (ii).the effect of toxic concentrations of three heavy metal compounds on the growth of the secondary callus tissue of nicotiana tabacum l. and ruta graveolens l. was studied. the metal compounds examined were znso4, niso4, cuso4. the metal compounds used were placed in murashige, skoog (1962) and white (1943) culture medium at 10(-6) and 10(-4) m concentration, respectively, before autoclaving. the culture media containing macro- and microelements and vitamins were completed with carbon source and r ...19883254011
protein inclusions produced by the entomopathogenic bacterium xenorhabdus nematophilus subsp. nematophilus.the entomopathogenic bacterium xenorhabdus nematophilus subsp. nematophilus produces two types of intracellular inclusion bodies during in vitro culture. large cigar-shaped inclusions (designated type 1) and smaller ovoid inclusions (designated type 2) were purified from cell lysates, using differential centrifugation in discontinuous glycerol gradients and isopycnic density gradient centrifugation in sodium diatrizoate. the inclusions, composed almost exclusively of protein, are readily soluble ...19873667532
audiological development in south-east asia. thailand health profile.the otological centre:bangkok unit has been established since march 13, 1985 on the initiation of isa and ifos as one of the planned three centres for each continent of the world. bangkok centre serves the asian countries on the global programme on prevention of hearing impairment and deafness. it was the first of the planned three centres to be established and operative. the centre has already run annual training courses to upgrade oto-audiological knowledge of ent doctors and personnel engaged ...19883187388
retinal toxicity of combination antiviral drugs in an animal model.we investigated the retinal toxicity of various combinations of four antiviral agents in intravitreal injection and vitrectomy infusion solutions in the rabbit. the nontoxic concentration of each individual agent for intravitreal injection and for vitrectomy infusion has previously been described. our results support the concept that concentrations of 200 micrograms/0.1 ml of trifluridine, 400 micrograms/0.1 ml of hydroxyacyclovir, 200 micrograms/0.1 ml of acyclovir and 30 micrograms/0.1 ml of v ...19873123041
a new sialoglycoprotein from rat seminal vesicle and its association with semen coagulum.by radiolabeling using naio4/[3h]kbh4, a new sialoglycoprotein with mr of 19,000 was found in the secretion of rat seminal vesicle. it was shown to interact non-covalently with semen coagulum. it existed in three acidic forms with pi values of 4.1, 3.7 and 2.9 and possessed high contents of sialic acids and acidic amino acids.19863707590
distribution of immunoreactive inhibin like material in different regions of human prostate.distribution of immunoreactive inhibin material (ir-ilm) in different regions of human prostate was studied by using specific radio-immunoassay. mean ir-ilm concentrations in the central, peripheral and periurethral zones were 27.3 +/- 9.3, 2.4 +/- 0.7 and 3.0 +/- 1.5 micrograms/mg protein respectively (p central vs periurethral and peripheral less than 0.05).19873688474
Displaying items 1 - 100 of 573