the cathode bound group antigen of dysentery-provoking escherichieae (author's transl).antigens from disrupted cells of dysentery-provoking and of non-enteropathogenic escherichieae were submitted to immunoelectrophoresis on cellulose acetate stripes at ph 8.0. among 6 immune sera produced for this purpose by immunizing rabbits against desintegrated dysentery bacteria, only one contained a precipitine reacting with an antigen similar to the "generic antigen" of belaya. this - at ph 8.0 - cathode-bound group antigen (kga) could not only be found in virulent but also in 5 attenuated ...1975873
use of lincomycin, methicillin and ristomycin in the nutrient media for isolating pathogenic intestinal microorganisms.elective-differentiating solid nutrient media for simultaneous isolation of vibrioes, salmonella and shigella were developed. antibiotics active against grampostive microflora and dry bile salts inhibiting the growth of proteus were used as the inhibitors of the growth of the accompanying microflora. the medium was lincomycin and the bile salts may be prepared in a dry form.19752096
lactulose therapy in shigella carrier state and acute dysentery.antibiotic-resistant shigella are increasingly prevalent. lactulose, a non-absorbable disaccharide, was investigated as an alternative therapy for shigella infection on the hypothesis that the short-chain fatty acids (inhibitory to shigella) resulting from metabolism of lactulose by normal colonic flora would diminish shigella excretion. a long-term antibiotic-refractory carrier (large bowel) excreting 10(4) to 10(7)shigella sonnei/g of feces was given two courses of lactulose (of 24 and 16 days ...19752098
comparative in vitro studies of cinoxacin, nalidixic acid, and oxolinic acid.cinoxacin and nalidixic acid were found to be similar in in vitro activity against 138 shigella isolates and somewhat less active than oxolinic acid on a weight basis. cross-resistance developed when 10 shigellae were transferred on increasing amounts of the respective agent contained in mueller-hinton agar. plate dilution studies of the effect of changes in agar ph on the minimum inhibitory concentration revealed that the antibacterial activity increased with decreasing ph. protein binding inve ...197612717
kallikrein inhibitors in rat plasma.the kallikrein inhibitor contents of human and animal plasma were determined with glandular kallikreins ec one ml of plasma could inactivate 20-700 kallikrein units (ku). rat plasma was the most potent and inactivated 230-700 ku. however, no enzyme capable of inactivating kallikrein could be found in this plasma. two fractions which inhibited hog pancreatic kallikrein, a fraction corresponding to alpha2-macroglobulin and a fraction which was eluted prior to albumin, were separated from ...197714935
purification and characterization of a plasminogen activator secreted by cultured human pancreatic carcinoma cells.a plasminogen activator secreted by cultured human pancreatic carcinoma (mia paca-2) cells has been purified to apparent homogeneity by procedures including sepharose-l-arginine methyl ester affinity chromatography, sephadex g-200 gel filtration, isoelectric focusing, and sodium dodecyl sulfate gel electrophoresis. the plasminogen activator shares many properties with urokinase including: molecular weight (55 000), isoelectric point (8.7), heat stability (60 degrees c, 30 min), ph stability (1.5 ...197715590
acute bacteremia in asplenic renal transplant patients.four cases of overwhelming bacteremia were observed in asplenic renal transplant patients 12 to 20 months after transplantation. the bacteriologic findings and presentation of these infections are characteristic of post-splenectomy sepsis reported in nontransplant patients. it is suggested that the absence of the spleen, more than immunosuppression with azathioprine and prednisone, predisposes to these late, uncommon infections after transplantation.197716144
identification of essential histidine residues of aminoacylase by photooxidation and by reaction with diethylpyrocarbonate.state and function of the histidine residues of aminoacylase were investigated by photoxidation in the presence of methylene blue and by chemical modification with diethylpyrocarbonate. complete inactivation of the enzyme was observed after oxidation of 4 histidine residues. from the ph dependence of the photooxidation it becomes evident that the inactivation of the enzyme is not a consequence of the simultaneous oxidation of tryptophan residues. the enzyme is also inactivated by chemical modifi ...197717959
detection of salmonella antigens in the neutralization reaction (anr). 197717975
testicular androgen binding protein (abp) - a parameter of sertoli cell secretory function.using abp as an index of sertoli cell secretory function, several important features of the sertoli cell have emerged: 1. the stimulation of abp production by fsh clearly points to the sertoli cell as a target cell for fsh (3,4,9-16,21,24). 2. the dramatic effects of androgens on abp production both in immature and mature rats also suggest that the sertoli cell is a target cell for androgen (3,12,14,16,25). 3. the striking reduction in abp production in the cryptorchid testis raises the question ...197518322
effect of oligomycin on coupling in isolated mitochondria from oligomycin-resistant mutants of saccharomyces cerevisiae carrying an oligomycin-resistant atp phosphohydrolase. 197720055
role of phospholipids in muscarinic binding by neural membranes. 197720109
drug effects on blood pressure and heart rate in unanesthetized animals. (1). effects of beta-blocking agents (author's transl).beta-blocking actions of orally administered kö 1400 and tiprenolol, new beta-blocking agents, were studied in unanesthetized rats and dogs, using a fall of blood pressure and an increase in heart rate produced by isoproterenol as a measure of beta-receptor activation. blood pressure was recorded from the aorta of the dog and the caudal artery of rat via indwelling catheter, and heart rate of the dog was recorded by a cardiotachometer triggered by r waves of the lead ii electrocardiogram. mean r ...197720392
liver enzyme adaptation after lithium administration in handled and nonhandled rats.we have examined the interaction of lithium administration and the infant stimulation procedure of handling on hormonally regulated enzymes of liver. animals handled in infancy show an increased morning corticosterone level in response to lithium feeding and markedly elevated serum glucose during refeeding following a two day fast, when compared to non-handled control animals. lithium alters serum corticosterone both in response to the stress of fasting, and during the diurnal cycle following gl ...197721840
calf liver nuclear n-acetyltransferases. purification and properties of two enzymes with both spermidine acetyltransferase and histone acetyltransferase activities.calf liver contains two nuclear n-acetyltransferases which are separated by chromatography on hydroxylapatite. both acetyltransferase a and acetyltransferase b will transfer acetate from acetyl-coa to either histone or spermidine. the same protein catalyzes the reaction with both substrates; this is shown by a constant ratio of spermidine to histone activity over a 5,000-fold purification and identical heat denaturation kinetics for both spermidine and histone acetyltransferase activity with eac ...197822543
proteolytic enzymes of the k-1 strain of streptomyces griseus obtained from a commercial preparation (pronase). effect of ph, metal ions, and amino acids on aminopeptidase activity. 197822544
beta adrenergic blockade, regional left ventricular blood flow and st-segment elevation in canine experimental myocardial ischemia.the effects of dl-propranolol, d-propranolol, dl-pindolol and dl-practolol on regional myocardial blood flow (assessed by means of tracer microspheres) and on st-segment elevation in ischemic and nonischemic areas of the canine left ventricle have been investigated. dl-propranolol and dl-pindolol, but not dl-practolol and d-propranolol, induced blood flow redistribution from the epicardium to the endocardium both in ischemic and nonischemic areas. dl-propranolol-induced redistribution was abolis ...197826795
environment and selfsufficiency of elder man (author's transl).the problems of environment of elderly people are concentrated in social area and in the intimate living space, which can not be maintained sufficiently because of increasing age and decreasing performance. the consequences of mobility in industrial revolution scattered families and also deprived old men of their helpers. inadequate living rooms and absence of technical comfort especially in the houses of the very aged men produce intense dependence of the own individual performance. medical dat ...197827118
neurochemical and operant behavioral studies of a strain of alcohol-preferring rats.the levels of serotonin (5-ht), 5-hydroxyindoleacetic acid, tyrosine (tyr), norepinephrine (ne), acetylcholine, gaba, glutamate (glu), aspartate (asp), alanine, glycine (gly) and taurine were measured in the cns of adult male rats selectively inbred to the f8 generation for alcohol preference (p) and non-preference (np). with respect to the values found in the np group, higher levels of 5-ht, gaba, glu and gly and lower levels of asp were found in the diencephalon-mesencephalon (d-m) and higher ...197827820
hepatic microsomal metabolism of epinephrine and adrenochrome by superoxide-dependent and -independent pathways. 197931892
the uptake of choline by streptococcus pneumoniae.uptake of choline, a structural component of pneumococcal c- and f-teichoic acids, into bacteria growing in a defined medium was very efficient with an uptake constant ([s]10 5) of 3.2 microns. it was inhibited by iodoacetate, dinitrophenol and oligomycin but not by structural analogues of choline. ethanolamine, however, was transported in the absence of choline but with a reduced affinity ([s]0.5 71.4 microns). the same constitutive system was probably used by both ethanolamine and choline. it ...197834010
study on the ion-permeability of human dental enamel with special reference to an electrochemical approach. 197734011
pseudomonas arthritis following puncture wounds of the foot. 197934012
effects of water soluble splenic extracts on intestinal smooth muscle. 197834364
[comparative study of interferon production in mice with the graft versus host reaction].mice with graft versus host reaction (gvhr) show a decreased production of serum interferon and that produced by the bone marrow and spleen cells and blood leukocytes in vitro upon inoculation with newcastle disease virus. interferon induction with lipopolysaccharide of flexner bacteria resulted in activation of production of serum interferon and that induced in spleen cell and blood leukocyte suspensions. serum interferon production after administration of poly(i) . poly(c) was similar in mice ...197834929
pharmacokinetics of the new beta-adrenoceptor blocker sl 75212 in man after repeated oral administration [proceedings]. 197941560
[proceedings: new method for chronic implantation of cortical electrodes]. 197554071
[study of the ability of shigella sonnei and flexneri to inactivate antibiotics]. 197554495
[study of the role of type-specific antigen in the process of shigella flexneri interaction with epithelial cell and macrophage cultures].epithelial cells of the hep-2 line infected in parallel by genetically connected strains of sh. flexneri, differing in the capacity to synthesize the type antigen were studied morphologically. the type antigen proved to show no significant influence on the penetration of dysentery bacilli into the cell. a study of the process of phagocytosis of the mentioned strains by a culture of peritoneal macrophages of guinea pig demonstrated that the presence of a type antigen communicated to the bacteria ...197555022
[obtaining purified preparations of individual shigella newcastle k- and o-antigens by using an ultracentrifugation method].a modified method of obtaining the purified k- and o-antigens of sh. newcastle with the use of ultracentrifugation is described. serologically individual k- and o-antigens were obtained and their chemical composition was studied. polysaccharide of the k-antigen consisted of the galacturonic acid, galactose, glucose, rhamnose xylose and hexosamine; it contained no heptoses. the o-antigen contained galactose, glucose, mannose, rhamnose, heptose and hexosamine; in difference to k-antigen was equal ...197555023
levamisole in advanced human breast cancer.a clinical trial of levamisole, an orally effective modifier of the immune response, is reported in women with primary inoperable breast cancer (stage iii). after being rendered clinically disease-free by radiotherapy to the breast, supraclavicular area, and axilla, patients were allocated alternately to a control group (no further treatment) and a levamisole-treated group (150 mg orally three times a week on alternate weeks) and were followed-up by physical examination and laboratory tests. in ...197655529
[sensitivity of dysentery bacteria to furazolidone and phthalazol according to the materials of medical epidemiological station of odessa-kishinev railroad]. 197658090
[localization on the shigella flexneri f6s chromosome of a rfa gene implicated in the biosynthesis of bacterial cell wall lipopolysaccharide]. 197558625
[receptor sites for phages specific for r mutants of shigella flexneri]. 197558628
[the metabolism of 4-14c-pregnenolone in tissue sections of human ovaries and their modification by chlormadinone acetate].in vitro incubations with slices of two normal human ovaries and 4-14c-pregnenolone as precursor were carried out to study the possibility of a direct influence of chlormadinone acetate on the metabolism of pregnenolone. in agreement with our previous studies the incubations of the ovary from the follicle phase of the cycle yields a profile of steroids different from that of the ovary from the corpus luteum phase of the cycle. under the experimental condition chosen, the presence of enzymes of ...197558782
the cutaneous reactions to kallikrein, prostaglandin and thurfyl nicotinate in chronic urticaria and the effect of polyphloretin phosphate.the erythema and wealing resulting from the application of thurfyl nicotinate ointment (trafuril) and from the inoculation of kallikrein has been studied in patients with chronic urticaria and normal controls. polyphloretin phosphate (ppp) suppressed the reaction in controls but in patients with urticaria it increased the reactions to trafuril and had little effect on the kallikrein reaction. ppp also suppressed the pge2-induced erythma in normal controls but not in urticaria patients. in a sepa ...197659506
studies on the pathogenesis of enteric infections caused by invasive bacteria.salmonellae, shigellae and some escherichia coli must invade the intestinal epithelial cell and multiply within the mucosa to cause disease. although the bacterial cell most likely possesses several properties essential to this invasive ability, the nature of the cell envelope complex is at present the only characteristic which has been implicated in this process. while a number of pathophysiological events result from invasion, some of our recent efforts have concerned the site and mechanism of ...197662646
[several allergic reactions under conditions of functioning of an inhibitor of antibody activity in the body].allergization under conditions of functioning an inhibitory factor in the organism was studied by the reaction of leukocytolysis with tsuverkalov's dysenterin and in the reproduction of hypersensitivity of the immediate type on the schultz-dale's model. it was shown that in functioning an inhibitory factor in the organism the degree of leukocytolysis reaction with the dysentery allergen decreased in the immunized animals in parallel with the fall in the serum antibody activity. a depression of t ...197765882
[role of several functional groups in the biological activity of allergenic preparations]. 197666777
[study of genetic control of synthesis of type iii and 6th group-specific antigens of flexner shigellae].as revealed in experiments of interspecific and intraspecific crosses, the determinant responsible for the group-specific 6, as well as controling the synthesis of type iii antigens of sh. flexneri were localized near the lac-pro markers on the chromosome. in intraspecies crosses of bacteria of y variant with the donor sh. flexneri 3c strain the percentage of linking of lac+a6+ was 32, and of lac+aiii+ --24. genetic confirmation of the dependence of the function of the a6 (defined as att6) deter ...197768643
whipple's disease: case report with immunological studies.a patient with whipple's disease is described whose small bowel biopsy demonstrated antigenicity to rabbit antisera to groups a, b, c, and g streptococci and shigella flexneri, but not to antisera to pneumococcus or shigella sonnei by immunofluorescent techniques. in addition, the patient's lymphocytes responded normally to phytohemagglutinin and to concanavalin a. these studies support the idea what whipple's disease is mediated by a bacteria-like agent which shares certain antigenic similariti ...197878871
lipopolysaccharides of shigella flexneri 6 as phage bacteriophages specifically lytic for representative strains of shigella flexneri serotype 6 were studied. cross hemagglutination experiments and chemical analysis of isolated lipopolysaccharides showed that strains 975 and 323 are smooth and strains 288, 551 and 488--rough. lipopolysaccharides isolated from the smooth strains inactivated phages f10 and "a" whereas those from the rough strains demonstrated poor inactivation of specific bacteriophages. the lipopolysaccharides of both the sm ...197880932
[production of antibodies against shigella flexneri antigen types i and ii by means of affinity chromatography]. 197882304
[solubilization by chlorpromazine of gamma glutamyltransferase of the plasma and microsomal membranes of rabbit liver (proceedings)]. 197884593
immunochemical localization of nitrate reductase in escherichia coli [proceedings]. 197884772
staining of legionella pneumophila. 197985128
[mast-cell degranulation in the intestine: its significance for the pathogenesis of galactosamine hepatitis. histological and histochemical studies on mast-cells using toluidine-blue and subsequent naphthol-asd-chloracetate reaction (author's transl)]. 197885385
goitrogens. 197985506
comparison of the temperature-dependence of rapid axonal transport and microtubules in nerves of the rabbit and bullfrog.1. the average velocity of transport of dopamine-beta-hydroxylase, an enzyme confined to unmyelinated adrenergic nerves, was measured in rabbit and bullfrog sciatic nerves or branches incubated in vitro at various temperatures. in parallel experiments, the number and density of microtubules were measured in cross-sections of randomly selected unmyelinated axons from another set of nerves incubated under the same conditions. 2. average transport velocity was exponentially related to temperature o ...197985705
immunochemical characteristics of shigella sonnei and serotype 6 shigella flexneri lipopolysaccharides and enterobacterial common antigen.immunochemical studies on shigella sonnei and serotype 6 shigella flexneri 0 antigens (lipopolysaccharides) and enterobacterial common antigen (eca) isolated from shigella sonnei were carried out. oligosaccharide structure of 0-specific chain of serotype 6 shigella fiexneri lipopolysaccharide was defined and beta-l-rhamnosyl-1,3-n-acetyl-d-galactosamin as immunodeterminant of type vi specificity was recognized. the structures of core regions of lipopolysaccharides isolated from r mutants of both ...197886336
definition of two ld antigens in rhesus monkeys.two rhesus (macaca mulatta) monkey lymphocyte-defined (ld) antigens have been identified using two typing cells as stiumlators in a one-way mixed leukocyte culture (mlc) assay. an analysis of the genetic behavior of these ld antigens in six rhesus monkey families revealed that both antigens were linked with rhla. one probable recombinant indicated that the ld locus lies outside the two known rhla-sd loci and the locus which controls the serum protein, properdin b(bf). these two antigens, ld1 and ...197987026
similarity of casein- and endotoxin-induced, myeloma- associated and aged sjl/j amyloid in various strains of mice.amyloidosis was induced in a number of strains of mice by repeated injections of casein and endotoxin. spontaneous amyloid was obtained from balb/c mice bearing a myeloma tumor (igg2a producing mopc 173 tumor) and from aged sjl/j mice. both the induced and spontaneous forms were similar in their size, immunological reactivity, peptide maps and in the susceptibility of histological sections to oxidizing agents with or without trypsin digestion. since case-induced murine amyloid resembles the noni ...197987374
moloney virus-induced cell surface antigens and histocompatibility antigens are located on distinct molecules. 197987439
some progress with nasopharyngeal carcinoma. 197987621
4th international workshop on human gene mapping. report of the committee on the genetic constitution of the x and y chromosomes. 197888299
immunofluorescent staining of platelet suspensions and detection of antiplatelet antibody in patients with idiopathic thrombocytopenic immunofluorescent staining of 31 specimens of platelets obtained from 13 cases of idiopathic thrombocytopenic purpura (itp) revealed positive staining on the surface of platelets for both immunoglobulins (igs) and human b1c globulin in 9 specimens, for only igs in 1 specimen and for human b1c alone in 5 specimens. the pattern of the positive immunofluorescent staining was granular. indirect immunofluorescent staining of normal platelets in serum obtained from patients with itp was positiv ...197988810
differentiation between cyclic amp level and allergic histamine release in mast cells [proceedings]. 197988891
[post-extrasystolic and post-pacing potentiation. i. experimental study of the isolated heart of the rabbit]. 197888947
autonomic block, cardiovascular depression and histamine release produced by polymyxin b in the cat.the present study quantifies the autonomic block, the cardiovascular depression and the histamine releasing effects of polymyxin b in nine anaesthetized cats. the dose requirements for 50 per cent depression of the mean arterial blood pressure, the bradycardiac response to vagal stimulation, and the contraction of the nictitating membrane elicited by pre-ganglionic and post-ganglionic stimulation of the cervical sympathetic trunk have a narrow range of scatter, being of the order of 6--12 ...197989005
reaction of cells from early chick embryos with antiserum against mouse sperm cells. 197989009
nonspecific suppressor cell activity and specific cellular unresponsiveness in rat schistosomiasis. 197989037
immunological unresponsiveness in mice. ii. cellular basis of immunological unresponsiveness induced in foetal and neonatal mice by transfer of human gamma-globulin by the maternal route.the cellular basis of the mechanism of immunological tolerance to human gamma-globulin (h gamma g) induced in foetal and neonatal mice by materno-foetal or materno-neonatal transfer after a single injection of tolerogen (deaggregated h gamma g) into the mothers was investigated using a cell transfer system and assays of passive haemagglutinating antibodies and plaque-forming cells to h gamma g. the results demonstrated that b cells are mainly involved in the tolerance induced on the fourteenth d ...197989080
preliminary studies on nasal decongestant activity from the seed of the shea butter tree, butyrospermum parkii.1 the seed of butyrospermum parkii yields shea butter which according to local traditional healers relieves inflammation of the nostrils. 2 since there is as yet no absolutely satisfactory nasal decongestant in clinical use, it was decided to investigate the effects of shea butter in nasal congestion. the substance was prepared in the laboratory. 3 the human subjects used were those suffering from rhinitis with moderate to severe nasal congestion. they were divided into the test group which rece ...197989854
the cellular basis of transplantation tolerance in the rat. 197990010
selective vascular thrombosis induced by a direct electrical current: animal experiments. first clinical trials. 197890140
characterization of an rna-directed dna-polymerase from a cell line derived from a radiation-induced lymphoma in rna-directed dna polymerase was purified from a cell line derived from a radiation-induced lymphoma in nih swiss mice which produced non-infectious type c virus particles. the enzyme was isolated from a high speed particulate fraction which bands at a density of 1.16--1.19 g/ml in a sucrose gradient, and purified by successive chromatography on deae-cellulose, phosphocellulose and hydroxyapatite. the purified dna polymerase has a molecular weight of 68 000, a ph optimum of 7.5, a kcl optimum ...197990522
complement profiles in monkeys subjected to aggregate (immune complex) anaphylaxis, and following injection of soluble and particulate polysaccharides.complement profiles were established in four groups of macaca irus monkeys: (i) aggregate (immune complex) anaphylaxis was induced following immunization, with ovalbumin. upon challenge, systemic arterial pressure decreased from 115 to 50 mm hg (mean values) in 10 min. the complement profiles revealed decreases in: c1q to less than 10% of initial value within 5 min; c4 proportional to hypotension; c3 slowly to 60% at 24 h; c5, c6, c7, c8 and factor b to about 80% of initial value in 5--30 min. c ...197990656
[neural secretion of histamine by the gastric mucosa and its effects on gastric motility]. 197890747
prolonged survival of corneal allografts in rabbits treated with cyclosporin a. 197990771
maternal blocking factors in human pregnancy are found in plasma not serum.pregnancy plasma and sera were evaluated for differential blocking effects in mixed lymphocyte culture reactions. autologous pregnancy plasma, but not serum was found to contain a specific allogeneic inhibitor for paternal lymphocytes.197990919
[vital staining of retina and uvea with trypan blue--particularly in comparison with neutral red staining (author's transl)]. 197991316
connectivity of the auditory forebrain nuclei in the guinea fowl (numida meleagris).injection of tritiated leucine and proline into the nucleus ovoidalis of the guinea fowl (numida meleagris) produces terminal labeling in the palaeostriatum and in three adjacent zones (field l1-l3) of the auditory neostriatum (an). l2, situated between l1 and l3, receives the main input and corresponds to the former field l of rose. these neuroanatomically defined zones of the auditory neostriatum are also characterized by differing properties of their neurons. injection of radioactive material ...197991442
antigen specific t cell-mediated suppression. vi. properties of in vitro generated l-glutamic acid60-l-alanine30-l-tyrosine10 (gat)-specific t-suppressor factor(s) (gat-tsf) in responder mouse strains. 197991643
[therapeutic and preventive aspects of diseases caused by pneumocystis carinii in children]. 197892047
horizontal organization of velocity characteristics in area 18 of the cat [proceedings]. 197992238
[influence of a dialyzable serum factor on the activity of beta-galactosidase from human liver (proceedings)]. 197992267
immunologic regulation in pregnancy.theories to explain the failure of rejection of the fetus by the mother during pregnancy include immunologic privilege of the uterus as a graft site, lack of transplantation antigen expression on the trophoblast, weakening of maternal cellular immunity during pregnancy, and separation of maternal and fetal circulations. evidence for and against each of these theories is discussed. local concentration of a variety of hormones, including human chorionic gonadotropin (hcg), sex steroids, alpha-feto ...197992318
the relation between the concentration of placental specific proteins in retroplacental and peripheral blood.concentrations of four placental proteins: human placental lactogen (hpl), placental protein 5 (pp5), pregnancy specific beta 1 glycoprotein (sp1) and human chorionic gonadotrophin (hcg), and a normal serum component, alpha 2 macroglobulin, were measured in the peripheral circulation and in blood obtained from the retroplacental space in 20 women at term delivery. levels of hpl and pp5 were higher in the retroplacental blood than in the peripheral circulation in all patients. by contrast, levels ...197992339
guinea-pig alpha 1 microglobulin. isolation and properties in comparison with human alpha 1 microglobulin. 197992407
nonspecific light loss and intrinsic dna variation problems associated with feulgen dna cytophotometry.nonspecific light loss by the cell-wall-plus-cytoplasm (cwc) can cause a 50% increase in feulgen absorption units in peanut root-tip nuclei as determined by scanning at 450 nm, whereas this phenomenon is not evident with chicken erythrocytes. a two wavelength scanning method of subtracting nonspecific 450 nm absorption from 550 nm feulgen absorption values eliminated the nonspecific light loss in cwc, however, the two wavelength scanning method is time consuming and somewhat impractical with a r ...197992496
immunoelectron microscopic localization of herpes simplex virus antigens in infected cells using the unlabeled antibody-enzyme method.subcellular localization of viral antigens was demonstrated during viral morphogenesis using herpes simplex virus type 1 (hsv-1) infected monolayers of rabbit cornea cells. the localization was done by immunoelectron microscopy employing the peroxidase-antiperoxidase (pap) immunocytochemical technique and the postembedding staining method. the localization of viral antigens was followed at time intervals during infection from 2 to 19 hr. after exposure of sections to either polyspecific antibodi ...197992500
[modified method of obtaining washings of the large intestine for determining coproantibodies and immunoglobulins in the dynamics of acute dysentery].the study of the washings obtained during rectoscopy from the mucous membrane of the large intestine with the use of a special attachment for a rectoscope revealed that this method had a number of advantages when compared with the study of coprofiltrates; in particular, this method allowed to obtain pure secretions of the mucous membrane free of fecal admixtures and to concentrate the gamma globulin fraction. the study of the dynamics of coproantibody secretion in acute dysentery revealed that c ...197992867
[relations between oxoglutarate transport function and variations of space in rat heart mitochondria (proceedings)]. 197992958
selective aromatic-hydrophobic binding and fractionation of immunoglobulin by means of phenyl-(ch2)n-nh-substituted agaroses. 197992988
[determination of the c-reactive protein content of human whole blood]. 197993165
human alphafetoprotein 1956-1978. 197993405
effect of azacytidine on simian virus 40 nucleoprotein complexes.simian virus 40 nucleoprotein complexes synthesized in the presence of 5-azacytidine showed small differences in sedimentation rate on neutral sucrose and buoyant density in metrizamide and cesium chloride. simian virus 40 deoxyribonucleic acid (dna) i, isolated from the nucleoprotein complexes of drug-treated cultures, was found to band at a higher buoyant density and therefore had a decreased ability to bind ethidium bromide. the data indicated that these molecules were deficient in superhelic ...197993427
evidence in vitro for a participation of the arginine repressor in cumulative repression of escherichia coli carbamoylphosphate synthase [proceedings]. 197993462
a novel class of schizosaccharomyces pombe mutants, phenotypically unable to grow on glycerol, a respiratory substrate, but still able to oxidize glycerol aerobically [proceedings]. 197993469
effects of intrathecal and intracerebroventricular injections of substance p on nociception in the rat and mouse [proceedings]. 197993501
surface-associated host proteins on virulent treponema pallidum.a surface coat of host serum proteins was detected on virulent treponema pallidum by sodium dodecyl sulfate-gel electrophoresis. the loosely associated serum proteins could be removed by repeated washings in a protein-free medium. washed t. pallidum retained the ability to readsorb numerous host proteins from rabbit serum as well as iodinated rabbit or human albumin. in addition, various avidly associated host serum proteins including albumin, alpha(2)-macroglobulin, transferrin, ceruloplasmin, ...197993574
antigenic heterogeneity of the non-serogroup antigen structure of neisseria gonorrhoeae lipopolysaccharides.studies of the antigenic structure of the polysaccharide component of gonococcal lipopolysaccaride (lps) indicated that the non-serogroup antigen structure is antigenically heterogeneous. immunodiffusion studies of gc4 strain 8551 indicated that in addition to the gc serogroup determinent, this polysaccharide contains two other sets of determinants, one which is shared with the other five gc serogroups and a second which is shared by the gc1 and gc3 serogroups. enzyme-linked immunosorbent assay ...197993575
abortive expression of the epstein-barr virus (ebv) cycle in a variety of ebv dna-containing cell lines, as reflected by nucleic acid hybridization in situ.a variety of epstein-barr virus (ebv) dna-containing cell lines have been tested for the expression of the ebv-associated antigens ebna (nuclear antigen), ea (early antigen), and vca (viral capsid antigen), and for the presence of cells containing disproportionate amounts of ebv dna. the antigen tests utilized immunofluorescence and 125i-labelled antibodies combined with autoradiography. ebv-dna was detected by in situ hybridization with 3h-labelled ebv rna complementary to p3hr-1 ebv dna (p-ebv ...197993584
characteristics and genetic control of nk-cell-mediated cytotoxicity activated by naturally acquired infection in the mouse.most and perhaps all natural-cell-mediated cytotoxicity (ncmc) may be activated by a response to exogenous infections. specific-pathogen-free (spf) mice have little or no natural killer (nk) cell activity, but when they are relocated in conventional conditions, they develop strong ncmc within 2 to 3 days. unlike other spf animals, hypothymic nude mice display good ncmc which is further augmented upon their entry into a pathogenic environment. the ontogeny, genetic control and other features of p ...197993585
alpha-fetoprotein inhibits antibody binding to acetylcholine receptor. significance for myasthenia gravis and other autoimmune diseases. 197993589
1-isoamyl-3-isobutylxanthine: a remarkably potent agent for the potentiation of norepinephrine, histamine, and adenosine-elicited accumulations of cyclic amp in brain slices. 197993674
in vivo release of substance p in the cat substantia nigra.push-pull cannuale were implanted into the substantia nigra (sn) and the caudate nucleus (cn) of the cat to study the in vivo release of substance p (sp), using a radioimmunoassay and high pressure liquid chromatography (hplc) analysis. the spontaneous release of sp could be detected in the sn and the cn. potassium (50 mm) locally applied stimulated sp release in both structures. furthermore an important evoked release of sp was observed in the sn during depolarization of striato-nigral sp fiber ...197993729
characterization and mode of action of a bacteriocin produced by a bacteroides fragilis strain.a bacteroides fragilis strain produces a low-molecular-weight (13,500 to 18,700), proteinaceous bacteriocin during the stationary growth phase. the extracellular bacteriocin is not inducible by ultraviolet light or mitomycin c and is stable between ph 7.5 and 8.2. the majority of the bacteriocin is thermolabile, but a small proportion (3%) of the bacteriocin is stable after autoclaving at 121 degrees c for 15 min. killing of sensitive bacteroides cells follows single-hit kinetics, and the intera ...197993876
effects of exogenous gonadotropic and steroid hormones on the social behaviour and gonadal maturation of male domestic ducklings anas platyrhynchos l.male domestic ducklings were injected during their first month of life with mammalian gonadotrophins (ovine lh or fsh, hmg) or gonadal steroids (testosterone or oestradiol). lh and testosterone stimulated sexual behaviour while oestradiol inhibited the increase of aggression observed in control birds during the experiment. the mammalian gonadotrophins did not increase plasma testosterone but nevertheless they all stimulated the testis growth. several hypotheses which could explain this finding ( ...197993890
the lac-test, a rapid method for the detection of agents inducing sos-dna repair in esch erichia coli [proceedings]. 197993911
Displaying items 1 - 100 of 11070