effects in the rat of intradermal injection of purified proteinases from streptococcus and serratia marcescens.purified streptococcal proteinase and serratia proteinase are potent permeability factors in rat skin and initiate histopathological evidence of an acute inflammatory response. these effects appear to be largely independent of terminal components of complement, histamine, and polymorphonuclear leukocytes.19751798
incorporation of proline into prodigiosin by a put mutant of serratia marcesens.a put mutant of serratia marcescens, deficient in proline oxidase and therefore unable to degrade proline, was used to assay for an enzymatic reaction responsible for incorporation of proline into prodigiosin. the reaction had a ph optimum of 7.5 and a km of 1.1 x 10(-4) m at 27 c. at temperatures above 27 c, the velocity of the reaction decreased with increasing temperature and little activity was detected at 42 c. activity of the enzyme was directly proportional to the quantity of pigment form ...19765953
kinetic properties of serratia marcescens adenosine 5'-diphosphate glucose pyrophosphorylase.the regulatory properties of partially purified adenosine 5'-diphosphate-(adp) glucose pyrophosphorylase from two serratia marcescens strains (atcc 274 and atcc 15365) have been studied. slight or negligible activation by fructose-p2, pyridoxal-phosphate, or reduced nicotinamide adenine dinucleotide phosphate (nadph) was observed. these compounds were previously shown to be potent activators of the adpglucose pyrophosphorylases from the enterics, salmonella typhimurium, enterobacter aerogenes, e ...19766432
inositol dehydrogenase from serratia marcescens. 19768876
antibacterial activity of sisomicin in comparison with gentamicin.the antibacterial activity of sisomicin -- a new aminoglycoside antibiotic -- as compared with gentamicin was tested on 521 bacterial strains of different species in a serial-dilution test. staphylococci, streptococci, e. coli, klebsialla-enterobacter, indole-psitive proteus strains, pseufomonads, salmonads, salmonellae, and serratia marcescens were inhibited to the extent of 100% at a maximun of 4.0 mug/ml. sisomicin showed a higher antibacterial activity against part of the bacterial species. ...19769099
factors influencing the immune enhancement of intrapulmonary bactericidal mechanisms.the effect of specific immunization on the antibacterial defense mechanisms of the murine lung was assessed against streptococcus pneumoniae, staphylococcus aureus, staphylococcus aureus (smith), serratia marcescens, klebsiella pneumoniae, proteus mirabilis, and pseudomonas aeruginosa. immunization by aerosol inhalation significantly enhanced the intrapulmonary killing of pseudomonas aeruginosa and proteus mirabilis but not the remaining organisms. with p. mirabilis, systemic immunization induce ...19769364
in vitro study of netilmicin compared with other aminoglycosides.netilmicin (sch 20569) is an ethyl derivative of gentamicin c(1a) that is active against most enterobacteriaceae, pseudomonas aeruginosa, and staphylococcus aureus isolates. among 342 clinical isolates tested, all staphylococci; 92% of escherichia coli, 93% of klebsiella pneumoniae, and 92% of enterobacter were inhibited by 0.8 mug or less of netilmicin per ml, but only 78% of p. aeruginosa were inhibited by 3.1 mug or less per ml. most clinical isolates of enterococci, serratia marcescens, and ...197610829
exocellular proteases of serratia marcescens and their toxicity to larvae of galleria mellonella.out of 18 strains of serratia marcescens producing exocellular proteases the strain serratia marcescens cceb 415 was selected according to preliminary experiments. it could be shown that the train exhibits proteolytic activity reaching up to 10 tu per 1 ml of the culture filtrate in a medium with gelatine and peptone. two proteolytic enzyme could be demonstrated by means of specific inhibitors edta and diisopropyfluorophosphate: metalprotease with optimum activity at ph 7.5 and serine protease w ...197611154
approach to a practical method for screening and identifying microorganism genera from urine (author's transl).in this study the author reported upon a practical new system for screening and identifying the microbial agents causing urinary tract infections. this system is composed of a combination of 3 screening procedures (ph-value + nitrite-test + catalase-test) and 8 selective culture media for the purpose of genus identification within 24 hours (uripret-g). a total of 130 cultures was investigated. the employed microorganisms were mainly recovered from urine samples. they included the following speci ...197611179
serum bactericidal activity in the horseshoe crab, limulus polyphemus.serum from the horseshoe crab, limulus polyphemus, was examined for bactericidal activity against five species of bacteria. greatest activity was found against pseudomonas putida and flavobacterium sp.; with the former, serum dilutions as high as 1:20 were capable of reducing viable counts by 50% within 2 h. bactericidal activity of a significantly lesser magnitude was demonstrated against serratia marcesencs and salmonella minnesota. no killing was seen when the lobster pathogen aerococcus viri ...197611188
unique aspects of the regulation of the aspartate transcarbamylase of serratia marcescens.aspartate trancarbamylase (atc ase; ec from serratia marcescens hy has been purified 134-fold. its properties are unique. unlike the atcase from escherichia coli and salmonella typhimurium, the s. marcescens hy enzyme activity is not feedback inhibited by any purine or pyrimidine nucleotide effectors; instead, the enzyme is activated by both cytidine 5'-triphosphate and adenosine 5'-triphosphate. like the atcase from e. coli and s. typhimurium, adenosine 5'-triphosphate alters the s0.5 ...197611207
on the effect of particulate airborne substances on various micro-organisms (author's transl).experimental studies on the reaction of micro-organisms on fine-dust filters (type c) and high-efficiency particulate air filters (type r and s) have shown no evidence of a growth of microorganisms. therefore efforts were aimed at detecting antimicrobial factors which were supposed to exist in the precipitated acidic continental aerosol. with this in mind, air filters from different locations and with service times were extracted in water and the extract was tested for its antimicrobial effects. ...197611621
biosynthesis of chitinase by achromobacter liquefaciens.bacterial cultures under study synthesize exocellular chitinase on a medium containing chitin or demineralized crab shells as a source of carbon and nitrogen. conditions for biosynthesis of chitinase by the cells of achromobacter liquefaciens 301a were investigated under periodic and continuous conditions of cultivation. the preparation of chitinase isolated from the cultural broth of a. liquefaciens 301a hydrolysed colloid and native chitin at the optimum ph 6.5 and temperature 40degreesc. the ...197612452
the extracellular metalloprotease of serratia marcescens: i. purification and extracellular protease of serratia marcescens produced during growth on skim milk medium was isolated by ethanol precipitation. the protease was purified by salt fractionation, deae-cellulose ion exchange chromatography and gel filtration chromatography on agarose p-100. it has a broad optimum from ph 6.0 to 9.0 and a temperature optimum of 45 degrees c for proteolytic activity on casein. it was classified as a metallo-protease by virtue of its inactivation by metal-ion chelators and reactiva ...197612465
role of prodigiozan in the overall therapy of dysentery in children. 197613573
selective association of spectrin with the cytoplasmic surface of human erythrocyte plasma membranes. quantitative determination with purified (32p)spectrin.a specific association between spectrin and the inner surface of the human erythrocyte membrane has been examined by measuring the binding of purified 32pspectrin to inside out, spectrin-depleted vesicles and to right side out ghost vesicles. spectrin was labeled by incubating erythrocytes with 32pi, and eluted from the ghost membranes by extraction in 0.3 mm napo4, ph 7.6. 32pspectrin was separated from actin and other proteins and isolated in a nonaggregated state as a so20,w = 7 s (in 0.3 mm ...197715998
effect of amino acids on the synthesis of extracellular protease in serratia marcescens. 197716200
identification of essential histidine residues of aminoacylase by photooxidation and by reaction with diethylpyrocarbonate.state and function of the histidine residues of aminoacylase were investigated by photoxidation in the presence of methylene blue and by chemical modification with diethylpyrocarbonate. complete inactivation of the enzyme was observed after oxidation of 4 histidine residues. from the ph dependence of the photooxidation it becomes evident that the inactivation of the enzyme is not a consequence of the simultaneous oxidation of tryptophan residues. the enzyme is also inactivated by chemical modifi ...197717959
phosphate inhibition of secondary metabolism in serratia marcescens.the synthesis of prodigiosin by non-proliferating cells of serratia marcescens was examined in the presence of a wide range of concentrations of inorganic phosphate (pi). a high elevation of pigment formation was obtained at less than or equal to 0.3 mm, and a broader but much lower elevation was obtained at 10 to 250 mm pi. the synthesis of two immediate precursors of the pitment also was inhibited by pi. the mechanism of action of pi did not involve changes in ph or accumulation of the trace m ...197718084
effect of cadmium on fungi and on interactions between fungi and bacteria in soil: influence of clay minerals and ph.fungi (rhizopus stolonifer, trichoderma viride, fusarium oxysporum f. sp. conglutinans, cunninghamella echinulata, and several species of aspergillus and penicillium) tolerated higher concentrations of cadmium (cd) when grown in soil than when grown on laboratory media, indicating that soil mitigated the toxic effects of cd. in soil amended with clay minerals, montmorillonite provided partial or total protection against fungistatic effects of cd, whereas additions of kaolinite provided little or ...197718085
comparison of two dioxygenases from pseudomonas putida.catechol 2,3-dioxygenase and homoprotocatechuate 2,3-dioxygenase were purified from the same strain of pseudomonas putida. molecular weights and subunit sizes were similar, but amino acid compositions showed some marked differences.197719416
cross-protective immunity to gram-negative bacilli: studies with core glycolipid of salmonella minnesota and antigens of streptococcus pneumoniae.two immunoprophylactic approaches to the control of infections caused by gramnegative bacilli were evaluated by study of experimental infections in animals. the core glycolipid antigen derived from the re mutant of salmonella minnesota r595 is shared by virtually all enteric bacteria, and immunization with this endotoxin protects against the hemodynamic sequelae of bacterial infection and pyrexia without enhancing intravascular clearance of bacteria. the degree of protection afforded by active a ...197719538
bacterial lipopolysaccharides as inducers of disease resistance in tobacco.the cell wall component of pseudomonas solanacearum that induces disease resistance in tobacco was highly heat stable at neutral or alkaline ph but highly labile at acid ph. activity was unaffected by nucleases and proteases but destroyed by a mixture of beta-glycosidases. washing of bacterial cell walls released a lipopolysaccharide (lps) fraction with high inducer activity. purified lps, extracted by a variety of procedures from whole cells, isolated cell walls, and culture filtrates of both s ...197721613
importance of the components of the woman's milk for infants in the neonatal period. 197722196
calf liver nuclear n-acetyltransferases. purification and properties of two enzymes with both spermidine acetyltransferase and histone acetyltransferase activities.calf liver contains two nuclear n-acetyltransferases which are separated by chromatography on hydroxylapatite. both acetyltransferase a and acetyltransferase b will transfer acetate from acetyl-coa to either histone or spermidine. the same protein catalyzes the reaction with both substrates; this is shown by a constant ratio of spermidine to histone activity over a 5,000-fold purification and identical heat denaturation kinetics for both spermidine and histone acetyltransferase activity with eac ...197822543
role of complement in lethal bacterial lipopolysaccharide-induced hypotensive and coagulative changes.the effect of c3 depletion on the multiple pathophysiological changes produced by a lethal dose of serratia marcescens lipopolysaccharide (lps) was evaluated. the injection of this lps into rabbits resulted in biphasic hypotensive changes and thrombocytopenia. these changes were characterized by an acute, transient decrease occurring within minutes after injection followed by a second more gradual decrease beginning 30 to 60 min post-lps. prior depletion of c3, with the anticomplementary protein ...197824001
bacterial 2,3-butanediol dehydrogenases.enterobacter aerogenes, aeromonas hydrophila, serratia marcescens and staphylococcus aureus possessing l(+)-butanediol dehydrogenase produced mainly meso-butanediol and small amounts of optically active butanediol; acetobacter suboxydans, bacillus polymyxa and erwinia carotovora containing d(-)-butanediol dehydrogenase produced more optically active butanediol than meso-butanediol. resting and growing cells of these organisms oxidezed only one enantiomer of racemic butanediol. the d(-)-butanedio ...197825056
evidence for quantitative variability of bacterial opsonic requirements.we studied human serum opsonins by using combinations of heat inactivation and chelation to inhibit complement, adsorption to remove antibody, and trypan blue to inactivate the c3 receptor of human polymorphonuclear leukocytes. streptococcus pneumoniae, serotype 25, required both complement and immunoglobulin for opsonization, even though that strain activated the alternative complement pathway. both strains of escherichia coli required antibody and complement, but varied in the degree of depend ...197825243
piperacillin: in vitro evaluation.the in vitro activity of a new semisynthetic penicillin, piperacillin, was determined against 577 clinical isolates of gram-positive cocci and gram-negative bacilli. a concentration of 12.5 mug/ml inhibited 92% of isolates of pseudomonas aeruginosa, 82% of serratia marcescens, 73% of escherichia coli, 61% of klebsiella spp., and 42% of enterobacter spp. most proteus spp. were extremely susceptible; over 85% were inhibited by 0.10 mug/ml. piperacillin failed to inhibit the growth of gram-negative ...197828694
dipeptidylaminopeptidase and carboxypeptidase activities of the brush border of rabbit small intestine. 197830675
[chitinolytic activity of the pigmented strains of serratia marcescens and of their pigment-free variants]. 197831204
kinetics of milk lipoprotein lipase. studies with tributyrin. 197832030
precipitate produced by serratia marcescens on macconkey agar: useful diagnostic test.the production of a precipitate by serratia marcescens on oxoid macconkey agar has proven useful as a laboratory diagnostic test. this phenomenon is specific for serratia within the enterobacteriaceae, although precipitate production is also given by acinetobacter anitratus and some pseudomonas, alcaligenes, and aeromonas species. precipitate production seems to be specific for certain batches of macconkey agar, and is probably related to a specific property of some batches of bile salts.197832188
effects of systemic and intracerebral administration of adrenergic receptor blocking drugs on the conditioned avoidance behavior and maze learning in rats. 197834842
rate of demethylation of methylmercuric chloride by enterobacter aerogenes and serratia marcescens. 197936188
purification and characterization of a serratia marcescens extracellular, nonelastolytic, neutral metalloprotease of serratia marcescens was purified by sequential ammonium sulfate precipitation, hydroxyapatite adsorption chromatography, flat-bed isoelectric focusing, and sephadex g-100 gel filtration. the protease preparation had a 280/260 nm absorbance ratio of 1.8, was free of detectable amounts of endotoxin, carbohydrate, phosphorus, and other known extracellular enzymes of s. marcescens, and was homogeneous by ouchterlony double immunodiffusion ...197937166
ps-5, a new beta-lactam antibiotic. ii. antimicrobial, a new beta-lactam antibiotic, has relatively potent antimicrobial activity against gram-positive and gram-negative bacteria, especially the enterobacter groups, serratia marcescens, the proteus groups and klebsiella pneumoniae. the activity of ps-5 against many beta-lactamase-producing organisms is greater than that of cefoxitin or cefazolin. ps-5 has good therapeutic activity in mice infected with staphylococcus aureus smith or enterobacter cloacae 45.197938238
[effect of cultivation conditions on lipase biosynthesis by serratia marcescens].the effect of cultivation conditions (the composition of the growth medium, ph, temperature) on lipase biosynthesis was studied with serratia marcescens 345 and serratia marcescens 348. the composition of the medium was found to be of key importance for lipase synthesis; the highest lipolytic activity of s. marcescens 345 was detected on the medium with 5% soybean flour broth while that of s. marcescens 348 was displayed on mpb. sodium salts of cholic acids added at different concentrations to t ...197941166
serratia marcescens as a pathogen of tsetse flies.when applied to the ears of rabbits used as hosts for tsetse flies, the bacterium serratia marcescens produced significant mortality in populations of glossina m. morsitans and g. pallidipes. after being ingested during the blood meal, cells of s. marcescens multiplied in the intestine of the flies and entered the hemocoel. using the brush method of applying the bacterium, 100% mortality of both glossina species occurred within 10 days after application. in newly killed flies, the bacteria could ...197943085
mammalian serum susceptibility of serratia marcescens: detection of three human serum susceptibility categories.a total of 65 strains of s. marcescens, including 36 currently employed o- and h-antigen reference strains, were examined for their susceptibility to the bactericidal activity of 80 vol % of fresh human serum. the majority of strains (57 = 87.6%) proved 'delayed serum-sensitive' (dss); 4 strains (6.2%) were 'promptly serum-sensitive' (pss), whereas 4 strains (6.2%) resisted (nss) complement-mediated killing by human serum. fresh sera obtained from 7 adult human volunteers yielded essentially ide ...197944784
the peptidyltransferase center of ribosomes. 197944920
further characterization of "promptly" and "delayed" human serum-sensitive strains of serratia marcescens.the kinetics of the bactericidal activity of 80 vol% of fresh human serum against representative 'delayed serum-sensitive' (dss) and 'promptly serum-sensitive' (pss) strains of serratia marcescens were further examined with regard to various chemical and absorption procedures known to affect various components of the alternative and classical pathways of human complement activation. inulin treatment of fresh human serum failed to diminish serum bactericidal activity against dss and pss assay str ...197944945
emergence of antibiotic resistance in hospitals, 1935-1975.a limited review of the changes in susceptibility of common bacterial pathogens to available antibacterial agents is presented. significant developments in recent years include the following: (1) the emergence of streptococcus pneumoniae with decreased resistance to penicillin and of some strains resistant to several antibiotics; (2) a decline in prevalence of multi-drug-resistant staphylococcus aureus after 1960 following their increasing prevalence in the preceding years (these changes were me ...197945521
immunological study of anthranilate immunological study of anthranilate synthetase (asase) has been initiated using quantitative precipitation, enzyme neutralization, and immunodiffusion methods. cross-reactivity of anthranilate synthetase-anthranilate-5-phosphoribosylpyrophosphate phosphoribosyltransferase (asase-prtase) from escherichia coli, klebsiella aerogenes, and salmonella typhimurium and asase from serratia marcescens and pseudomonas putida was detected with antibodies to ?e. coli trypsin-treated asase. cross-reactivit ...197550316
cell-mediated immune response in mice treated with steroidal contraceptives.the splenomegaly assay (simonsen, 1962) was standardized using different strains of rats and mice. male wistar rat (donor)-female swiss mouse (host) was found to be the suitable combination that could be employed in subsequent experiments to study the potential of contraceptive steroids to alter cmir. the index of splenomegaly appeared to increase in case of mice treated with combination oral contraceptives (ovulen, ovral or enovid). the differences observed, however, neared significance only in ...197555327
the thyroxine-binding properties of rat and rabbit serum proteins.the thyroxine (t4) binding properties of rat and rabbit serum proteins were studied using a gel equilibration technique, gel filtration and polyacrylamide gel electrophoresis. in both species two different t4 binding molecules were identified in whole serum and in cohn fraction v preparations. only one of these binding species demonstrated the characteristics of specific binding, i.e., high affinity for the hormone and binding site saturability. the mean (+/- sd) apparent association constants ( ...197655359
gentamicin-resistant pseudomonas aeruginosa and serratia marcescens in a general hospital.from aug. 1, 1974, to july 31, 1975, inclusive, 19-1% of pseudomonas aeruginosa and 50-0% of serratia marcescens isolates from a general hospital were gentamicin resistant as determined by standardised disc testing; 80% of 118 different clinical isolates showed minimum inhibitory concentrations larger than or equal to 16 mug/ml of gentamicin in agar-dilution testing. all gentamicin-resistant s. marcescens and 79% of ps. aeruginosa isolates were susceptible to amikacin, while tobramycin and sisom ...197655850
effects of hypothalamic lesions on levels of plasma free fatty acids in the mallard duck.plasma free fatty acid (ffa) levels were measured in the mallard duck (anas platyrhynchos) following hypothalamic lesions at various sites. the results indicate that ventromedial lesions produced hyperphagia, increased deposition of fat, and significantly elevated levels of plasma ffa. anterior bilateral lesions resulted in aphagia, severe loss in body weight and a marked decrease in plasma ffa. lesions in other regions of the hypothalamus produced various changes depending upon the extent of d ...197557744
a general method for the isolation of non-sense suppressor strains in gram-negative bacteria. 197558594
expiratory bacterial filters. 197659079
"5-amino-5"-deoxybutirosin, a semisynthetic analogue of butirosin a: antibacterial activity in vitro and in mice.aminodeoxybutirosin (ad-btn), the 5''-amino-5''-deoxy derivative of butirosin (btn), was synthesized to improve on the antibacterial activity of btn by preventing bacterial enzymatic phosphorylation at the 5'' position. ad-btn possesses the spectrum characteristic of btn and gentamicin (gtm) and was active at low levels in vitro against a wide variety of gram-negative species including pseudomonas aeruginosa, indole-positive proteus and serratia marcescens; its action was bactericidal against bo ...197662554
serratia marcescens in hospital practice. 197764829
serratia marcescens in hospital practice. 197765627
the importance of s. marcescens. 197766438
importance of serratia marcescens. 197767455
a polyvalent human gamma-globulin immune to pseudomonas aeruginosa: passive protection of mice against lethal a means to development of guidelines for therapeutic application to human disease, preparations of human polyvalent gamma-globulin immune to pseudomonas aeruginosa (pg) were studied in acute infections in mice. pg was highly effective in controlling lethal infections induced in mice by the major immunotypes of p. aeruginosa; greater than or equal to 10 microgram of of gamma-globulin per mouse protected against challenge with less than or equal to 10(6) 50% lethal doses of p. aeruginosa. pg wa ...197770492
source of american serratia. 197770675
[side effects of antibiotics in patients with thermal burns].the possibilities of antibacterial therapy in the clinics of burn diseases has at present decreased because of increasing microflora resistance to antibiotics. this phenomenon is one of the most often causes of antibacterial drug side effects in burn patients. for control of infections complications in burn patients which are most often lethal it is necessary to use biologically active subtance, such as prodigiozan and lysozime in addition to the directed antibiotic therapy. the use of specific ...197771876
[simple method of growing sporeless anaerobic microorganisms on flat media]. 197772883
[antigenic study of serratia marcescens isolated in france. i.--antigens: individualization of six new h factors (author's transl)].the h antigens of 469 strains of serratia marcescens have been studied. a specific immobilization test in soft agar with antiserum is described. being more quickly carried out than tube agglutination, it has the same specificity. the most frequently identified h antigen is h:12. six new h antigens (h:14 to h:19) have been individualized, one of them--h:17--frequently occurring in strains isolated in france.197773361
detection of serratia outbreaks in hospital.infections due to serratia marcescens were studied in 23 different hospitals. a retrospective study was done in 4 hospitals; all isolates were compared by serological typing, antibiograms, bacteriocin production, and bacteriocin sensitivity. 2 of the hospitals were having cross-infection problems due to antibiotic-resistant strains, but the other 2 had little or no cross-infection. outbreaks were studied in 19 other hospitals. 9 of these outbreaks were classified as "common source" since contam ...197673753
serotyping of serratia marcescens: determination of serogroup (o) antigens and serological cross-reactions. 197876382
phenotypic variations of bacteriocin susceptibility, o-antigen determinants, and serum susceptibility among isolates of a multiple-drug-resistant, nosocomially significant strain of serratia marcescens. 197876383
serratia marcescens colonising the gut. 197877407
purification and partial characterization of the b subunit of serratia marcescens tryptophan synthetase.a trpe mutant of serratia marcescens (e-7) was isolated, and the multimeric enzyme tryptophan synthetase (ec was purified to homogeneity from derepressed cells. the a and b subunits were resolved, and the b subunit was partially characterized and compared with the escherichia coli b subunit as part of a comparative evolution study of the trpb cistron of the trp operon in the enterobacteriaceae. the s. marcescens b subunit is a dimer (beta(2)), and its molecular weight was estimated to ...197877857
mutational biosynthesis of butirosin analogs. ii. 3', 4'-dideoxy-6'-n-methylbutirosins, new semisynthetic aminoglycosides.a pair of new butirosin analogs was isolated from the fermentation broth obtained by cultivating a neamine-negative mutant of the butirosin-producing organism bacillus circulans in the medium supplemented with 6'-n-methylgentamine c1a. these antibiotics were characterized and elucidated as 3', 4'-dideoxy-6'-n-methylbutirosins a and b (dmb-a & dmb-b), by chemical and spectroscopic studies. dmb-a and dmb-b exhibited broad-spectrum antibacterial activities with in vitro potency similar to or slight ...197881823
electron microscopic observations of polysaccharide components in polymyxin b treated outer membranes from serratia marcescens.treatment of outer membranes of serratia marcescens with polymyxin b results in the formation of blebs. this effect is thought to be due to the action of the antibiotic on the lipopolysaccharides, proteins, phospholipids or a combination thereof. it is unclear whether this effect is dissociative, degradative or due to an inhibition of the assembly of outer membrane components. prior studies showed that lipopolysaccharides and polymyxin b form complexes, but direct visualization of the in situ ac ...197983989
[effect of s-adenosyl-l-methionine on the cerebral and peripheral metabolism of l-dopa].the repartition of [3h]l-dopa administered i.p. does not change in rats treated with s-adenosyl-l-methionine (sam). the effect was studied on the central nervous system and at the periphery. the biosynthesis of metabolites [3h]dopamine and [3h]3-o-methyldopamine are also unchanged. on the other hand, in the kidney an accumulation of [3h]norepinephrine +[3h]normetanephrine was observed, while in the brainstem + midbrain the synthesis of these metabolites was decreased after sam injection. the rel ...197884550
study of the glycolipid composition of the milk fat-globule membrane and the mouse mammary tumour virus prepared from the milk of infected swiss mice [proceedings]. 197884579
[antigenic study of "serratia marcescens" isolated in france.ii.--characterization of o antigens and description of 5 new o factors, occurrence of serotypes and labelling of new h factors (author's transl)].the somatic o-antigens of 583 french isolates of serratia marcescens were studied with the aid of the tube o-agglutination technique sensitized by centrifugation. flagella antigens were identified by the h-immobilization test. five new o-antigens (o16 to o20) were found. an antigenic factor (co) common to isolates of serotypes o12:h9, o14:h12, o14:h20, and o13:h17 is described. agar cultures of serotypes o12:h9 and o14:h12 dissociated into two colonial types: iridescent colonies, and non-iridesc ...197885425
the influence of left ventricular filling on postextrasystolic potentiation in the dog heart. 197985504
cell-mediated immunity to epstein-barr virus in nasopharyngeal carcinoma. 197986139
the humoral and cell-mediated immune responses to somatic and metabolic antigens in rats infected with angiostrongylus cantonensis. 197886211
inhibition of neuronal firing by opiates: evidence against the involvement of cyclic nucleotides.1. extracellular recordings were made in vitro from single neurones of the myenteric plexus of the guinea-pig ileum. 2. neuronal firing was inhibited by morphine and normorphine (10 nm to 1 micrometer). cyclic adenosine 3',5'-monophosphate (cyclic amp) (100 micrometer to 1 mm) also inhibited the firing of the majority of the neurones. prostaglandin e2 usually caused a short-lasting excitation of myenteric neurones and the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine was usually withou ...197986371
studies of human anti-igm anti-igg cryoglobulins. i. patterns of reactivity with autologous and isologous human igg and its subunits.monoclonal human anti-igg preparations purified from mixed igm-igg cryoglobulins were tested for their antigenic specificity by haemagglutination-inhibition assay. a panel of fourteen igg preparations of the four gamma chain subclasses were prepared from myeloma sera and used as inhibitors of haemagglutination. each of six igm anti-globulins demonstrated different reactivity profiles with these igg preparations. in addition, the fraction of the serum igg which had bound to and cryoprecipitated w ...197986510
the phenomenon of differentiation in murine erythroleukemic cells. 197886529
cytofluorometric and cytochemical comparisons of normal and abnormal human cells from the female genital tract.acridine orange staining of exfoliated cells from epithelial tissues facilitates discrimination between normal and abnormal cells: abnormal cells develop highly elevated nuclear fluorescence. comparisons of acridine orange (ao) staining with propidium iodide (pi) or feulgen staining have shown that: (a) pi staining also provides highly elevated nuclear fluorescence from abnormal cells; (b) the distributions of nuclear fluorescence following ao or pi staining were usually not significantly differ ...197986579
ultrastructure of bacteroides species: bacteroides asaccharolyticus, bacteroides fragilis, bacteroides melaninogenicus subspecies melaninogenicus, and b. melaninogenicus subspecies intermedius.representative strains of two subspecies of bacteroides melaninogenicus (subspecies melaninogenicus and subspecies intermedius) and bacteroides asaccharolyticus as well as b. asaccharolyticus strain 536b isolated from a human perirectal abscess and bacteroides fragilis atcc 25285 were examined by glutaraldehyde-osmium fixation, ruthenium red fixation and staining, and thorium hydroxyde staining as well as by the physical preparative techniques of critical point drying--transmission electron micr ...197986590
definition of two ld antigens in rhesus monkeys.two rhesus (macaca mulatta) monkey lymphocyte-defined (ld) antigens have been identified using two typing cells as stiumlators in a one-way mixed leukocyte culture (mlc) assay. an analysis of the genetic behavior of these ld antigens in six rhesus monkey families revealed that both antigens were linked with rhla. one probable recombinant indicated that the ld locus lies outside the two known rhla-sd loci and the locus which controls the serum protein, properdin b(bf). these two antigens, ld1 and ...197987026
potentiating effect of nitrate and other anions on contraction in vascular smooth muscle. 197887173
similarity of casein- and endotoxin-induced, myeloma- associated and aged sjl/j amyloid in various strains of mice.amyloidosis was induced in a number of strains of mice by repeated injections of casein and endotoxin. spontaneous amyloid was obtained from balb/c mice bearing a myeloma tumor (igg2a producing mopc 173 tumor) and from aged sjl/j mice. both the induced and spontaneous forms were similar in their size, immunological reactivity, peptide maps and in the susceptibility of histological sections to oxidizing agents with or without trypsin digestion. since case-induced murine amyloid resembles the noni ...197987374
moloney virus-induced cell surface antigens and histocompatibility antigens are located on distinct molecules. 197987439
immune suppression in vivo with antigen-modified syngeneic cells. ii. t cell-mediated nonresponsiveness to fowl gamma-globulin.intravenous administration of syngeneic spleen cells coupled with the palmitoyl derivative of fowl gammma-globulin (p-f gamma g) results in a profound state of f gamma g-specific tolerance in c57bl/6 mice. administration of p-f gamma g coupled syngeneic cells specifically reduces both the primary and secondary hapten and carrier-specific pfc responses to tnp-f gamma g. since the haptenic response is affected, the tolerance functions at the level of the f gamma g-specific helper t cell. as few as ...197987447
human corneal endothelium in organ culture. the influence of temperature and medium of incubation.forty-four human corneas from patients between 21 and 86 years were incubated in eagle's minimum essential medium with earle's salts 10--46 h post mortem. the influence of incubation temperature and composition of the medium on endothelial survival was evaluated. whole corneas were stained by alizarine red. recent cell loss was indicated by morphological alterations in the endothelial pattern. after 20--28 h of incubation minimum cell loss was found at 31 degrees c when 8% dextrane-250 and 20% s ...197988158
expression of endodermally derived and neural crest-derived differentiation antigens by human lung and colon tumors. 197988254
the role of p23,30-bearing human macrophages in antigen-induced t lymphocyte responses. 197988270
nucleolar organizing regions of human chromosomes.silver-stained cells from 49 parents with a history of several abortions were compared with cells from 35 parents with normal liveborn children. the modal and mean number of silver-stained nors (ag-nors) observed on d- or g-group chromosomes was similar in both groups and between males and females. ag-nors were randomly distributed on all five acrocentric pairs. the distribution and size of ag-nors within an individual was not random and was fairly consistent from cell to cell. the mean number o ...197988410
steroid receptor content in cytosol from normal and hyperplastic human prostates.analyses of steroid hormone receptors were performed using a dextran-coated charcoal technique in cytosolic preparations from 40 cases of benign prostatic hyperplasia (bph) and 10 normal human prostates. binding data were calculated according to scathchard. in all bph specimens, receptors for the synthetic androgen methyltrienolone (mt) were found (mean maximum number of binding sites, 566 fmol/mg dna; mean kd, 0.61 nm), and 25 of 28 samples contained progestin [17 alpha, 21-dimethyl-19-nor-4.8- ...197988456
a new assay of estrogen receptor by thin-layer gel filtration. 197988911
the pathophysiology of asthma.because postmortem studies of humans provide little information on the initial pathophysiologic events in asthma, animal models have been developed. recently the ascaris-allergic rhesus monkey has provided an opportunity to examine the onset of pathophysiologic changes following challenge and to correlate them with airway structure. these studies have suggested that the initial interaction between antigen and mast cells may occur in the bronchial lumen or in the epithelium superficial to the tig ...197989006
reaction of cells from early chick embryos with antiserum against mouse sperm cells. 197989009
preparative isolation of the cell receptor for immunoglobulin e. 197989114
control of v kappa expression in the mouse. i. unexpected expression of the v kappa allele, igk-pcb, in a somatic cell hybrid of akr (igk-pca) origin. 197989158
inheritance of tolerance susceptibility to human gamma-globulin in congenic mice.the genetic control of susceptibility to tolerance induction with human gamma-globulin (hgg) was studied by using h-2 congenic mice. strains tested that were congenic with c57bl/10sn were completely tolerized by 1.0 mg deaggregated hgg. in contrast a/sn mice showed full tolerance whereas a.sw mice were only intermediately tolerant. it was further shown that (b10 x sjl)f1 mice could be rendered tolerant but (b10.s x sjl)f1 mice could not. these data indicate a role for h-2 linked genes in control ...197989162
antigenic changes associated with liver carcinogenesis.carcinogen-induced experimental hepatomas are often characterized by new individually distinct antigens capable of inducing tumor immunity in syngeneic hosts. these antigens arise as a consequence of cell-carcinogen interaction and may result from modification or replacement of normal cell-surface components. their role in immunosurveillance is not established, but they offer a target for tumor immunotherapy. reexpressed fetal antigens have also been detected, either as secretory products (alpha ...197989200
antigenic analysis of human heart tissue--characterization of cardiac antigens.the human heart antigens demonstrated in citric acid extracts by immunodiffusion were submitted to further analysis. heart antigens were precipitated at 30% to 70% saturated ammonium sulphate. these fractions contained antigens reactive with rabbit antisera to human heart. the third fraction out of four proteins which were separated by sephadex g-150 gel filtration, was reactive against rabbit antisera to human heart. the intensely stained proteins of both heart antigens were located at the nine ...197989208
[change in the thresholds of development of ventricular arrhythmias after stimulation of negative emotiogenic hypothalamic centers].the dynamics of changes in the thresholds of the origin of ventricular extrasystole, paroxysmal ventricular tachysystole and ventricular fibrillation in long-term stimulation of the ventromedial hypothalamic nucleus were studied in experiments on rabbits. it was found that myocardial resistance to the development of arrhythmias depended on the manifestation of the sympathetico-parasympathic interaction on the heart. in predominance of parasympathetic effects on the heart during hypothalamic stim ...197989222
[use of herman's method of staining mycobacterium tuberculosis]. 197989264
cimetidine: interaction with oral anticoagulants in 6 patients anticoagulated with warfarin, nicoumalone, or phenindione the addition of cimetidine prolonged the prothrombin-time (pt) by a mean of 12.6 s (range 5--23 s). in 7 volunteers taking daily subtherapeutic doses of warfarin the addition of cimetidine increased the pt from 19.4 to 22.9 s and the plasma-warfarin concentration from 0.96 to 1.76 microgram/ml. cimetidine reduced the single-dose clearance of warfarin and antipyrine. the basis of the interaction between cimetidine and oral an ...197989387
[studies on human immunoglobulin preparations: i. viral antibody profiles (author's transl)].a study was undertaken to assess the viral antibody spectrum in human immunoglobulin preparations from four different manufacturers for intravenous and/or intramuscular application. studies of the kind seem to be reasonable for calculating the expected therapeutic effect of the administered immunoglobulin. the investigated 16 gammaglobulin batches showed almost the same variety of viral antibodies and only slightly differring antibody titers. it is interesting to note, that antibodies to vaccini ...197989620
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