| effects in the rat of intradermal injection of purified proteinases from streptococcus and serratia marcescens. | purified streptococcal proteinase and serratia proteinase are potent permeability factors in rat skin and initiate histopathological evidence of an acute inflammatory response. these effects appear to be largely independent of terminal components of complement, histamine, and polymorphonuclear leukocytes. | 1975 | 1798 |
| surface balance study of the interaction between microorganisms and lipid monolayer at the air/water interface. | using the surface balance technique, we have compared the interaction between acholeplasma laidlawii and some marine bacteria towards different types of monolayered lipid films. cells from a. laidlawii and serratia marinorubra penetrate the film, whereas cells from psuedomonas fluorescens form a layer underneath the film. the forces that bind microorganisms to the air/water interface are not strong enough to scatter a condensed monolayer but increase the strength of loosely packed monolayers. | 1976 | 5058 |
| gentamicin-susceptibility of various pathogens isolated from clinical materials. | we studied on the antibacterial activity of gentamicin against various pathogens isolated from clinical materials mainly isolated during 1974 and 1975, comparing with other antibiotics. beta hemolytic streptococci, pneumococci and enterococci are less susceptible to gentamicin than staphylococci. staph, aureus and staph. epidermidis resistant to various antibiotics are very susceptible to gentamicin, and no resistant strain to this drug was found. haemophilus influenzae, h. parainfluenzae and h. ... | 1976 | 5619 |
| incorporation of proline into prodigiosin by a put mutant of serratia marcesens. | a put mutant of serratia marcescens, deficient in proline oxidase and therefore unable to degrade proline, was used to assay for an enzymatic reaction responsible for incorporation of proline into prodigiosin. the reaction had a ph optimum of 7.5 and a km of 1.1 x 10(-4) m at 27 c. at temperatures above 27 c, the velocity of the reaction decreased with increasing temperature and little activity was detected at 42 c. activity of the enzyme was directly proportional to the quantity of pigment form ... | 1976 | 5953 |
| kinetic properties of serratia marcescens adenosine 5'-diphosphate glucose pyrophosphorylase. | the regulatory properties of partially purified adenosine 5'-diphosphate-(adp) glucose pyrophosphorylase from two serratia marcescens strains (atcc 274 and atcc 15365) have been studied. slight or negligible activation by fructose-p2, pyridoxal-phosphate, or reduced nicotinamide adenine dinucleotide phosphate (nadph) was observed. these compounds were previously shown to be potent activators of the adpglucose pyrophosphorylases from the enterics, salmonella typhimurium, enterobacter aerogenes, e ... | 1976 | 6432 |
| inositol dehydrogenase from serratia marcescens. | | 1976 | 8876 |
| antibacterial activity of sisomicin in comparison with gentamicin. | the antibacterial activity of sisomicin -- a new aminoglycoside antibiotic -- as compared with gentamicin was tested on 521 bacterial strains of different species in a serial-dilution test. staphylococci, streptococci, e. coli, klebsialla-enterobacter, indole-psitive proteus strains, pseufomonads, salmonads, salmonellae, and serratia marcescens were inhibited to the extent of 100% at a maximun of 4.0 mug/ml. sisomicin showed a higher antibacterial activity against part of the bacterial species. ... | 1976 | 9099 |
| factors influencing the immune enhancement of intrapulmonary bactericidal mechanisms. | the effect of specific immunization on the antibacterial defense mechanisms of the murine lung was assessed against streptococcus pneumoniae, staphylococcus aureus, staphylococcus aureus (smith), serratia marcescens, klebsiella pneumoniae, proteus mirabilis, and pseudomonas aeruginosa. immunization by aerosol inhalation significantly enhanced the intrapulmonary killing of pseudomonas aeruginosa and proteus mirabilis but not the remaining organisms. with p. mirabilis, systemic immunization induce ... | 1976 | 9364 |
| purification and properties of the dihydrofolate synthetase from serratia indica. | the dihydrofolate synthetase (ec 6.3.2.12) responsible for catalyzing the synthesis of dihydrofolic acid from dihydropteroic acid and l-glutamic acid was purified about 130-fold from extracts of serratia indica ifo 3759 by ammonium sulfate fractionation, deae-sephadex column chromatography, sephadex g-200 gel filtration, and deae-cellulose column chromatography. the enzyme preparation obtained was shown to be homogeneous by deae-cellulose column chromatography and ultracentrifugal analysis. the ... | 1976 | 9496 |
| in vitro study of netilmicin compared with other aminoglycosides. | netilmicin (sch 20569) is an ethyl derivative of gentamicin c(1a) that is active against most enterobacteriaceae, pseudomonas aeruginosa, and staphylococcus aureus isolates. among 342 clinical isolates tested, all staphylococci; 92% of escherichia coli, 93% of klebsiella pneumoniae, and 92% of enterobacter were inhibited by 0.8 mug or less of netilmicin per ml, but only 78% of p. aeruginosa were inhibited by 3.1 mug or less per ml. most clinical isolates of enterococci, serratia marcescens, and ... | 1976 | 10829 |
| exocellular proteases of serratia marcescens and their toxicity to larvae of galleria mellonella. | out of 18 strains of serratia marcescens producing exocellular proteases the strain serratia marcescens cceb 415 was selected according to preliminary experiments. it could be shown that the train exhibits proteolytic activity reaching up to 10 tu per 1 ml of the culture filtrate in a medium with gelatine and peptone. two proteolytic enzyme could be demonstrated by means of specific inhibitors edta and diisopropyfluorophosphate: metalprotease with optimum activity at ph 7.5 and serine protease w ... | 1976 | 11154 |
| approach to a practical method for screening and identifying microorganism genera from urine (author's transl). | in this study the author reported upon a practical new system for screening and identifying the microbial agents causing urinary tract infections. this system is composed of a combination of 3 screening procedures (ph-value + nitrite-test + catalase-test) and 8 selective culture media for the purpose of genus identification within 24 hours (uripret-g). a total of 130 cultures was investigated. the employed microorganisms were mainly recovered from urine samples. they included the following speci ... | 1976 | 11179 |
| serum bactericidal activity in the horseshoe crab, limulus polyphemus. | serum from the horseshoe crab, limulus polyphemus, was examined for bactericidal activity against five species of bacteria. greatest activity was found against pseudomonas putida and flavobacterium sp.; with the former, serum dilutions as high as 1:20 were capable of reducing viable counts by 50% within 2 h. bactericidal activity of a significantly lesser magnitude was demonstrated against serratia marcesencs and salmonella minnesota. no killing was seen when the lobster pathogen aerococcus viri ... | 1976 | 11188 |
| unique aspects of the regulation of the aspartate transcarbamylase of serratia marcescens. | aspartate trancarbamylase (atc ase; ec 2.1.3.2) from serratia marcescens hy has been purified 134-fold. its properties are unique. unlike the atcase from escherichia coli and salmonella typhimurium, the s. marcescens hy enzyme activity is not feedback inhibited by any purine or pyrimidine nucleotide effectors; instead, the enzyme is activated by both cytidine 5'-triphosphate and adenosine 5'-triphosphate. like the atcase from e. coli and s. typhimurium, adenosine 5'-triphosphate alters the s0.5 ... | 1976 | 11207 |
| the role of microflora in development of graft-versus-host disease. | | 1976 | 11583 |
| on the effect of particulate airborne substances on various micro-organisms (author's transl). | experimental studies on the reaction of micro-organisms on fine-dust filters (type c) and high-efficiency particulate air filters (type r and s) have shown no evidence of a growth of microorganisms. therefore efforts were aimed at detecting antimicrobial factors which were supposed to exist in the precipitated acidic continental aerosol. with this in mind, air filters from different locations and with service times were extracted in water and the extract was tested for its antimicrobial effects. ... | 1976 | 11621 |
| biosynthesis of chitinase by achromobacter liquefaciens. | bacterial cultures under study synthesize exocellular chitinase on a medium containing chitin or demineralized crab shells as a source of carbon and nitrogen. conditions for biosynthesis of chitinase by the cells of achromobacter liquefaciens 301a were investigated under periodic and continuous conditions of cultivation. the preparation of chitinase isolated from the cultural broth of a. liquefaciens 301a hydrolysed colloid and native chitin at the optimum ph 6.5 and temperature 40degreesc. the ... | 1976 | 12452 |
| the extracellular metalloprotease of serratia marcescens: i. purification and characterization. | an extracellular protease of serratia marcescens produced during growth on skim milk medium was isolated by ethanol precipitation. the protease was purified by salt fractionation, deae-cellulose ion exchange chromatography and gel filtration chromatography on agarose p-100. it has a broad optimum from ph 6.0 to 9.0 and a temperature optimum of 45 degrees c for proteolytic activity on casein. it was classified as a metallo-protease by virtue of its inactivation by metal-ion chelators and reactiva ... | 1976 | 12465 |
| role of prodigiozan in the overall therapy of dysentery in children. | | 1976 | 13573 |
| resistance to antibiotics of bacteria involved in respiratory infections (author's transl). | the present status of resistance to antibiotics of bacteria involved in respiratory infections is reviewed. schematically it can outlined as follows. streptococcus b-haemolyticus as well as pneumococcus did not change their sensitivity to penicillin, but some strains are now resistant to tetracycline. streptococcus viridans, enterococcus and h. influenzae did not change substantially their sensitivity to antibiotics. staphylococcus aureus is the bacterial species that always poses some problems ... | 1976 | 13744 |
| effect of amino acids on the synthesis of extracellular protease in serratia marcescens. | | 1977 | 16200 |
| azlocillin: in vitro studies of a new semisynthetic penicillin. | the activity of azlocillin, a new semisynthetic penicillin, was determined against 582 clinical isolates of gram-negative bacilli and gram-positive cocci. over 75% of the isolates of pseudomonas aeruginosa were inhibited at a concentration of 12.5 mug or less per ml. azlocillin is also active against indole-negative and -positive proteus spp., inhibiting 98 and 71%, respectively, at a concentration of 12.5 mug or less per ml. isolates of klebsiella spp. and enterobacter spp. showed less suscepti ... | 1977 | 18083 |
| phosphate inhibition of secondary metabolism in serratia marcescens. | the synthesis of prodigiosin by non-proliferating cells of serratia marcescens was examined in the presence of a wide range of concentrations of inorganic phosphate (pi). a high elevation of pigment formation was obtained at less than or equal to 0.3 mm, and a broader but much lower elevation was obtained at 10 to 250 mm pi. the synthesis of two immediate precursors of the pitment also was inhibited by pi. the mechanism of action of pi did not involve changes in ph or accumulation of the trace m ... | 1977 | 18084 |
| effect of cadmium on fungi and on interactions between fungi and bacteria in soil: influence of clay minerals and ph. | fungi (rhizopus stolonifer, trichoderma viride, fusarium oxysporum f. sp. conglutinans, cunninghamella echinulata, and several species of aspergillus and penicillium) tolerated higher concentrations of cadmium (cd) when grown in soil than when grown on laboratory media, indicating that soil mitigated the toxic effects of cd. in soil amended with clay minerals, montmorillonite provided partial or total protection against fungistatic effects of cd, whereas additions of kaolinite provided little or ... | 1977 | 18085 |
| cross-protective immunity to gram-negative bacilli: studies with core glycolipid of salmonella minnesota and antigens of streptococcus pneumoniae. | two immunoprophylactic approaches to the control of infections caused by gramnegative bacilli were evaluated by study of experimental infections in animals. the core glycolipid antigen derived from the re mutant of salmonella minnesota r595 is shared by virtually all enteric bacteria, and immunization with this endotoxin protects against the hemodynamic sequelae of bacterial infection and pyrexia without enhancing intravascular clearance of bacteria. the degree of protection afforded by active a ... | 1977 | 19538 |
| bacterial lipopolysaccharides as inducers of disease resistance in tobacco. | the cell wall component of pseudomonas solanacearum that induces disease resistance in tobacco was highly heat stable at neutral or alkaline ph but highly labile at acid ph. activity was unaffected by nucleases and proteases but destroyed by a mixture of beta-glycosidases. washing of bacterial cell walls released a lipopolysaccharide (lps) fraction with high inducer activity. purified lps, extracted by a variety of procedures from whole cells, isolated cell walls, and culture filtrates of both s ... | 1977 | 21613 |
| role of complement in lethal bacterial lipopolysaccharide-induced hypotensive and coagulative changes. | the effect of c3 depletion on the multiple pathophysiological changes produced by a lethal dose of serratia marcescens lipopolysaccharide (lps) was evaluated. the injection of this lps into rabbits resulted in biphasic hypotensive changes and thrombocytopenia. these changes were characterized by an acute, transient decrease occurring within minutes after injection followed by a second more gradual decrease beginning 30 to 60 min post-lps. prior depletion of c3, with the anticomplementary protein ... | 1978 | 24001 |
| bacterial 2,3-butanediol dehydrogenases. | enterobacter aerogenes, aeromonas hydrophila, serratia marcescens and staphylococcus aureus possessing l(+)-butanediol dehydrogenase produced mainly meso-butanediol and small amounts of optically active butanediol; acetobacter suboxydans, bacillus polymyxa and erwinia carotovora containing d(-)-butanediol dehydrogenase produced more optically active butanediol than meso-butanediol. resting and growing cells of these organisms oxidezed only one enantiomer of racemic butanediol. the d(-)-butanedio ... | 1978 | 25056 |
| evidence for quantitative variability of bacterial opsonic requirements. | we studied human serum opsonins by using combinations of heat inactivation and chelation to inhibit complement, adsorption to remove antibody, and trypan blue to inactivate the c3 receptor of human polymorphonuclear leukocytes. streptococcus pneumoniae, serotype 25, required both complement and immunoglobulin for opsonization, even though that strain activated the alternative complement pathway. both strains of escherichia coli required antibody and complement, but varied in the degree of depend ... | 1978 | 25243 |
| 1-n hapa gentamicin b, a new aminoglycoside active against gentamicin resistant isolates--activity compared to other aminoglycosides. | 1-n hapa gentamicin b is a new aminoglycoside active against most enterobacteriaceae, pseudomonas aeruginosa and staphylococcus aureus. among 504 clinical isolates at a concentration of 12.5 microgram/ml all staph. aureus, escherichia coli, klebsiella, enterobacter, proteus rettgeri, providencia and 78% of pseudomonas, 86% of proteus morganii were inhibited. like other aminoglycosides, the activity was greatest at an alkaline ph and reduced by high cations concentrations. 1-n hapa gentamicin b w ... | 1978 | 27494 |
| piperacillin: in vitro evaluation. | the in vitro activity of a new semisynthetic penicillin, piperacillin, was determined against 577 clinical isolates of gram-positive cocci and gram-negative bacilli. a concentration of 12.5 mug/ml inhibited 92% of isolates of pseudomonas aeruginosa, 82% of serratia marcescens, 73% of escherichia coli, 61% of klebsiella spp., and 42% of enterobacter spp. most proteus spp. were extremely susceptible; over 85% were inhibited by 0.10 mug/ml. piperacillin failed to inhibit the growth of gram-negative ... | 1978 | 28694 |
| studies of relationship among terrestrial pseudomonas, alcaligenes, and enterobacteria by an immunological comparison of glutamine synthetase. | antibody to purified glutamine synthetase from escherichia coli was prepared and used for an immunological comparison of glutamine synthetases from species of salmonella, citrobacter, enterobacter, serratia, proteus, erwinia, aeromonas, pseudomonas, acinetobacter, xanthomonas, alcaligenes, and paracoccus. the results of ouchterlony double diffusion experiments and quantitative microcomplement fixation studies indicated that the amino acid sequence of this enzyme was highly conserved in different ... | 1978 | 31146 |
| [chitinolytic activity of the pigmented strains of serratia marcescens and of their pigment-free variants]. | | 1978 | 31204 |
| precipitate produced by serratia marcescens on macconkey agar: useful diagnostic test. | the production of a precipitate by serratia marcescens on oxoid macconkey agar has proven useful as a laboratory diagnostic test. this phenomenon is specific for serratia within the enterobacteriaceae, although precipitate production is also given by acinetobacter anitratus and some pseudomonas, alcaligenes, and aeromonas species. precipitate production seems to be specific for certain batches of macconkey agar, and is probably related to a specific property of some batches of bile salts. | 1978 | 32188 |
| rate of demethylation of methylmercuric chloride by enterobacter aerogenes and serratia marcescens. | | 1979 | 36188 |
| microbial nitrosamine formation in palm wine: in vitro n-nitrosation by cell suspensions. | the ability of certain species of bacteria and yeasts that usually contaminate fresh palm sap to induce the formation of the carcinogen dimethylnitrosamine from suitable precursors (trimethylamine, dimethylamine, nitrate, and nitrite) was investigated in vitro under neutral and acid ph conditions. in the incubation media containing cell suspensions of each species of test organism, namely, aerobacter, micrococcus, serratia, saccharomyces, and two unidentified yeasts and the precursors, added in ... | 1979 | 36441 |
| purification and characterization of a serratia marcescens metalloprotease. | an extracellular, nonelastolytic, neutral metalloprotease of serratia marcescens was purified by sequential ammonium sulfate precipitation, hydroxyapatite adsorption chromatography, flat-bed isoelectric focusing, and sephadex g-100 gel filtration. the protease preparation had a 280/260 nm absorbance ratio of 1.8, was free of detectable amounts of endotoxin, carbohydrate, phosphorus, and other known extracellular enzymes of s. marcescens, and was homogeneous by ouchterlony double immunodiffusion ... | 1979 | 37166 |
| ps-5, a new beta-lactam antibiotic. ii. antimicrobial activity. | ps-5, a new beta-lactam antibiotic, has relatively potent antimicrobial activity against gram-positive and gram-negative bacteria, especially the enterobacter groups, serratia marcescens, the proteus groups and klebsiella pneumoniae. the activity of ps-5 against many beta-lactamase-producing organisms is greater than that of cefoxitin or cefazolin. ps-5 has good therapeutic activity in mice infected with staphylococcus aureus smith or enterobacter cloacae 45. | 1979 | 38238 |
| polymicrobial bacteremia. | of 26,961 blood cultures taken during an 18-month period at the cincinnati general hospital, 1,715 (6%) were positive. ninety-four patients had blood cultures containing more than one organism. although aerobic and anaerobic streptococci were the most frequently isolated bacteria, a variety of microorganisms, including staphylococcus aureus and the klebsiella-enterobacter-serratia group, was isolated in different combinations depending on the underlying disease. neurological illness, malignant n ... | 1979 | 40048 |
| [effect of cultivation conditions on lipase biosynthesis by serratia marcescens]. | the effect of cultivation conditions (the composition of the growth medium, ph, temperature) on lipase biosynthesis was studied with serratia marcescens 345 and serratia marcescens 348. the composition of the medium was found to be of key importance for lipase synthesis; the highest lipolytic activity of s. marcescens 345 was detected on the medium with 5% soybean flour broth while that of s. marcescens 348 was displayed on mpb. sodium salts of cholic acids added at different concentrations to t ... | 1979 | 41166 |
| serratia marcescens as a pathogen of tsetse flies. | when applied to the ears of rabbits used as hosts for tsetse flies, the bacterium serratia marcescens produced significant mortality in populations of glossina m. morsitans and g. pallidipes. after being ingested during the blood meal, cells of s. marcescens multiplied in the intestine of the flies and entered the hemocoel. using the brush method of applying the bacterium, 100% mortality of both glossina species occurred within 10 days after application. in newly killed flies, the bacteria could ... | 1979 | 43085 |
| mammalian serum susceptibility of serratia marcescens: detection of three human serum susceptibility categories. | a total of 65 strains of s. marcescens, including 36 currently employed o- and h-antigen reference strains, were examined for their susceptibility to the bactericidal activity of 80 vol % of fresh human serum. the majority of strains (57 = 87.6%) proved 'delayed serum-sensitive' (dss); 4 strains (6.2%) were 'promptly serum-sensitive' (pss), whereas 4 strains (6.2%) resisted (nss) complement-mediated killing by human serum. fresh sera obtained from 7 adult human volunteers yielded essentially ide ... | 1979 | 44784 |
| further characterization of "promptly" and "delayed" human serum-sensitive strains of serratia marcescens. | the kinetics of the bactericidal activity of 80 vol% of fresh human serum against representative 'delayed serum-sensitive' (dss) and 'promptly serum-sensitive' (pss) strains of serratia marcescens were further examined with regard to various chemical and absorption procedures known to affect various components of the alternative and classical pathways of human complement activation. inulin treatment of fresh human serum failed to diminish serum bactericidal activity against dss and pss assay str ... | 1979 | 44945 |
| emergence of antibiotic resistance in hospitals, 1935-1975. | a limited review of the changes in susceptibility of common bacterial pathogens to available antibacterial agents is presented. significant developments in recent years include the following: (1) the emergence of streptococcus pneumoniae with decreased resistance to penicillin and of some strains resistant to several antibiotics; (2) a decline in prevalence of multi-drug-resistant staphylococcus aureus after 1960 following their increasing prevalence in the preceding years (these changes were me ... | 1979 | 45521 |
| immunological study of anthranilate synthetase. | an immunological study of anthranilate synthetase (asase) has been initiated using quantitative precipitation, enzyme neutralization, and immunodiffusion methods. cross-reactivity of anthranilate synthetase-anthranilate-5-phosphoribosylpyrophosphate phosphoribosyltransferase (asase-prtase) from escherichia coli, klebsiella aerogenes, and salmonella typhimurium and asase from serratia marcescens and pseudomonas putida was detected with antibodies to ?e. coli trypsin-treated asase. cross-reactivit ... | 1975 | 50316 |
| effects of igm and igg antibody in patients with bacteremia due to gram-negative bacilli. | earlier studies, which indicated that high titers of o-specific antibody to the patient's infecting organism in acute-phase serum specimens were not associated with a decrease in the frequency of subsequent shock and death in bacteremia due to gram-negative bacilli, were reexamined for evaluation of the protective activity of specific igg and igm antibody. titers of hemagglutination antibody and levels of igm, determined by indirect immunofluorescent staining of the patient's infecting organism, ... | 1976 | 54397 |
| gentamicin-resistant pseudomonas aeruginosa and serratia marcescens in a general hospital. | from aug. 1, 1974, to july 31, 1975, inclusive, 19-1% of pseudomonas aeruginosa and 50-0% of serratia marcescens isolates from a general hospital were gentamicin resistant as determined by standardised disc testing; 80% of 118 different clinical isolates showed minimum inhibitory concentrations larger than or equal to 16 mug/ml of gentamicin in agar-dilution testing. all gentamicin-resistant s. marcescens and 79% of ps. aeruginosa isolates were susceptible to amikacin, while tobramycin and sisom ... | 1976 | 55850 |
| a general method for the isolation of non-sense suppressor strains in gram-negative bacteria. | | 1975 | 58594 |
| expiratory bacterial filters. | | 1976 | 59079 |
| "5-amino-5"-deoxybutirosin, a semisynthetic analogue of butirosin a: antibacterial activity in vitro and in mice. | aminodeoxybutirosin (ad-btn), the 5''-amino-5''-deoxy derivative of butirosin (btn), was synthesized to improve on the antibacterial activity of btn by preventing bacterial enzymatic phosphorylation at the 5'' position. ad-btn possesses the spectrum characteristic of btn and gentamicin (gtm) and was active at low levels in vitro against a wide variety of gram-negative species including pseudomonas aeruginosa, indole-positive proteus and serratia marcescens; its action was bactericidal against bo ... | 1976 | 62554 |
| serratia marcescens in hospital practice. | | 1977 | 64829 |
| serratia marcescens in hospital practice. | | 1977 | 65627 |
| the importance of s. marcescens. | | 1977 | 66438 |
| gentamicin-resistant serratia. | | 1977 | 66541 |
| importance of serratia marcescens. | | 1977 | 67455 |
| a polyvalent human gamma-globulin immune to pseudomonas aeruginosa: passive protection of mice against lethal infection. | as a means to development of guidelines for therapeutic application to human disease, preparations of human polyvalent gamma-globulin immune to pseudomonas aeruginosa (pg) were studied in acute infections in mice. pg was highly effective in controlling lethal infections induced in mice by the major immunotypes of p. aeruginosa; greater than or equal to 10 microgram of of gamma-globulin per mouse protected against challenge with less than or equal to 10(6) 50% lethal doses of p. aeruginosa. pg wa ... | 1977 | 70492 |
| source of american serratia. | | 1977 | 70675 |
| [side effects of antibiotics in patients with thermal burns]. | the possibilities of antibacterial therapy in the clinics of burn diseases has at present decreased because of increasing microflora resistance to antibiotics. this phenomenon is one of the most often causes of antibacterial drug side effects in burn patients. for control of infections complications in burn patients which are most often lethal it is necessary to use biologically active subtance, such as prodigiozan and lysozime in addition to the directed antibiotic therapy. the use of specific ... | 1977 | 71876 |
| [simple method of growing sporeless anaerobic microorganisms on flat media]. | | 1977 | 72883 |
| [antigenic study of serratia marcescens isolated in france. i.--antigens: individualization of six new h factors (author's transl)]. | the h antigens of 469 strains of serratia marcescens have been studied. a specific immobilization test in soft agar with antiserum is described. being more quickly carried out than tube agglutination, it has the same specificity. the most frequently identified h antigen is h:12. six new h antigens (h:14 to h:19) have been individualized, one of them--h:17--frequently occurring in strains isolated in france. | 1977 | 73361 |
| detection of serratia outbreaks in hospital. | infections due to serratia marcescens were studied in 23 different hospitals. a retrospective study was done in 4 hospitals; all isolates were compared by serological typing, antibiograms, bacteriocin production, and bacteriocin sensitivity. 2 of the hospitals were having cross-infection problems due to antibiotic-resistant strains, but the other 2 had little or no cross-infection. outbreaks were studied in 19 other hospitals. 9 of these outbreaks were classified as "common source" since contam ... | 1976 | 73753 |
| serotyping of serratia marcescens: determination of serogroup (o) antigens and serological cross-reactions. | | 1978 | 76382 |
| phenotypic variations of bacteriocin susceptibility, o-antigen determinants, and serum susceptibility among isolates of a multiple-drug-resistant, nosocomially significant strain of serratia marcescens. | | 1978 | 76383 |
| serratia marcescens colonising the gut. | | 1978 | 77407 |
| purification and partial characterization of the b subunit of serratia marcescens tryptophan synthetase. | a trpe mutant of serratia marcescens (e-7) was isolated, and the multimeric enzyme tryptophan synthetase (ec 4.2.1.20) was purified to homogeneity from derepressed cells. the a and b subunits were resolved, and the b subunit was partially characterized and compared with the escherichia coli b subunit as part of a comparative evolution study of the trpb cistron of the trp operon in the enterobacteriaceae. the s. marcescens b subunit is a dimer (beta(2)), and its molecular weight was estimated to ... | 1978 | 77857 |
| synthetic disaccharide-protein antigen for production of specific o2 antiserum for immunofluorescence diagnosis of salmonella. | antisera from rabbits immunized with the synthetic disaccharide paratose 1 leads to alpha 3 mannose, representive of salmonella o-antigen 2, covalently linked to bovine serum albumin (bsa), were used in indirect immunofluorescence studies for the identification of salmonella serogroup a (o-antigen 1,2,12) bacteria. among 1311 enteric bacteria tested, 497 were salmonella. the anti-paratose 1 leads to alpha 3 mannose-bsa serum identified correctly all the 63 serogroup a strains tested. no positive ... | 1978 | 78659 |
| mutational biosynthesis of butirosin analogs. ii. 3', 4'-dideoxy-6'-n-methylbutirosins, new semisynthetic aminoglycosides. | a pair of new butirosin analogs was isolated from the fermentation broth obtained by cultivating a neamine-negative mutant of the butirosin-producing organism bacillus circulans in the medium supplemented with 6'-n-methylgentamine c1a. these antibiotics were characterized and elucidated as 3', 4'-dideoxy-6'-n-methylbutirosins a and b (dmb-a & dmb-b), by chemical and spectroscopic studies. dmb-a and dmb-b exhibited broad-spectrum antibacterial activities with in vitro potency similar to or slight ... | 1978 | 81823 |
| electron microscopic observations of polysaccharide components in polymyxin b treated outer membranes from serratia marcescens. | treatment of outer membranes of serratia marcescens with polymyxin b results in the formation of blebs. this effect is thought to be due to the action of the antibiotic on the lipopolysaccharides, proteins, phospholipids or a combination thereof. it is unclear whether this effect is dissociative, degradative or due to an inhibition of the assembly of outer membrane components. prior studies showed that lipopolysaccharides and polymyxin b form complexes, but direct visualization of the in situ ac ... | 1979 | 83989 |
| [antigenic study of "serratia marcescens" isolated in france.ii.--characterization of o antigens and description of 5 new o factors, occurrence of serotypes and labelling of new h factors (author's transl)]. | the somatic o-antigens of 583 french isolates of serratia marcescens were studied with the aid of the tube o-agglutination technique sensitized by centrifugation. flagella antigens were identified by the h-immobilization test. five new o-antigens (o16 to o20) were found. an antigenic factor (co) common to isolates of serotypes o12:h9, o14:h12, o14:h20, and o13:h17 is described. agar cultures of serotypes o12:h9 and o14:h12 dissociated into two colonial types: iridescent colonies, and non-iridesc ... | 1978 | 85425 |
| [simplified and accelerated methods of determining the generic affiliation of bacteria of the intestinal bacilli group]. | | 1979 | 86647 |
| the strategy of infection as a criterion for phylogenetic relationships of non-coli phages morphologically similar to phage t7. | five phages which are morphologically similar to coliphage t7 but attack other host bacteria have been compared to t7 and to its relative, t3, by the following criteria: (a) cross-reactivity with antisera against t7 and t3, (b) dna base sequence homologies, as determined by the c0t technique, (c) synthesis of two phage-coded enzymes: rna polymerase and samase, (d) patterns of phage-directed protein synthesis, as determined by sds-polyacrylamide gel electrophoresis of phage coat subunits. as judg ... | 1979 | 90110 |
| problems associated with prolonged wear soft contact lenses. | complications of prolonged wearing of soft contact lenses are corneal infection, peripheral vascularization, giant papillary conjunctivitis and allergy, sterile ulcers, corneal warping, lens infection, protein and calcium deposits, lens fragility, and problems with disinfecting solutions. further long-term studies are needed to compare the safety of prolonged wear soft contact lenses with the safety of intraocular lenses, on which long-term studies are currently being done. | 1979 | 93736 |
| mezlocillin: a new acyl ureidopenicillin. antimicrobial activity and combination effects with four aminoglycoside antibiotics. | mezlocillin (baypen), carbenicillin and mezlocillin combined with gentamicin, sisomicin, tobramycin and amikacin, respectively, were tested in vitro against 325 fresh clinical isolates of pathogenic gram-negative bacteria using the tube dilution procedure. the new acyl ureidopenicillin has a greater antimicrobial activity than carbenicillin against nearly all species tested. the comparison of the effectiveness of the mezlocillin-aminoglycoside combinations demonstrates the superiority of mezloci ... | 1978 | 95925 |
| sk&f 75073, new parenteral broad-spectrum cephalosporin with high and prolonged serum levels. | sk&f 75073, a new parenteral cephalosporin, was found to have broad in vitro and in vivo antibacterial activity including isolates usually resistant to cephalothin and cefazolin. this activity included indole-positive proteus and enterobacter species and some serratia isolates. proteus mirabilis strains were particularly susceptible, as were haemophilus influenzae and neisseria species. the activity of sk&f 75073 against gram-positive bacteria was poorer than that of the control cephalosporins. ... | 1978 | 96734 |
| [action of some bacterial proteases on calf kidney cells cultured in vitro]. | | 1977 | 98380 |
| in vitro susceptibility of gentamicin and/or tobramycin resistant gram-negative bacilli to seven aminoglycosides. | the in vitro activity of gentamicin, tobramycin, sisomicin, netilmicin, amikacin, kanamycin and streptomycin was tested simultaneously by the agar dilution method against 584 clinical isolates of gram-negative bacilli that were resistant to gentamicin and/or tobramycin. about half of the gentamicin-resistant pseudomonas were susceptible to tobramycin but cross-resistance was virtually complete between gentamicin and tobramycin for enterobacteriaceae. sisomicin was much more active than gentamici ... | 1978 | 98451 |
| strain-dependent cytotoxic effects of endotoxin for mouse peritoneal macrophages. | the cytotoxic effects of bacterial lipopolysaccharides (lps) on mouse leukocytes have been examined in vivo and in vitro. intraperitoneal injection of lps into c57bl/6 mice greatly reduced the recovery of mononuclear cells; lps was cytotoxic for macrophages, but had a mitogenic effect on lymphocytes. similar effects of lps on peritoneal leukocytes were observed in vitro. when monolayers of adherent peritoneal cells were studied in vitro, cytotoxicity was also observed, suggesting that the effect ... | 1978 | 101460 |
| [bacteriocin (marcescin) typing of serratia marcescens. ii. the distribution of serratia marcescens in hospital and its isolated cases by bacteriocin (marcescin) typing (author's transl)]. | | 1978 | 101627 |
| quantitative assessment of bactericidal activities of beta-lactam antibiotics by agar plate method. | quantitative bactericidal activities of beta-lactam antibiotics were determined by the agar plate method. broth cultures, of which the colony-forming units were counted before the study, were inoculated on antibiotic-containing agar plates, utilizing a 10(-3), 10(-2), or 10(-1) dilution or undiluted culture plated with each 0.001-ml calibrated loop. these plates were incubated at 37 degrees c overnight, and the minimal drug concentration at which no bacterial growth was observed on the plates wa ... | 1978 | 102246 |
| [species specific esterase profiles in the genus serratia]. | esterases of 62 serratia marcescens, s. liquefaciens, s. plymuthica and s. marinorubra strains were analysed by electrophoresis in polyacrylamide-agarose gel. the comparative distribution of bands showed that the four serratia species were characterized by distinct electrophoretic patterns of their esterases. | 1978 | 102456 |
| susceptibility of the anaerobic bacteria, group d streptococci, enterobacteriaceae, and pseudomonas to semisynthetic penicillins: carbenicillin, piperacillin, and ticarcillin. | sodium piperacillin t-1220, a new semisynthetic penicillin, was tested in vitro against 297 clinical isolates of anaerobic bacteria and 669 aerobic bacteria by the conventional agar dilution method and compared with carbenicillin and ticarcillin. at a 100-mug/ml concentration the three drugs showed comparable effectiveness against the anaerobes tested. however, at 20 mug/ml, piperacillin was the most effective drug against bacteroides fragilis, peptostreptococci, and group d streptococci. at thi ... | 1978 | 103491 |
| dry-heat destruction of lipopolysaccharide: dry-heat destruction kinetics. | dry-heat destruction kinetics of lipopolysaccharides from escherichia coli, serratia marcescens, and salmonella typhosa at 170 to 250 degrees c are described. the destruction rate seems to follow the second order and can be linearized by the equation, log y = a + b . -10cx. because c is the slope, 1/c = d3. both a and b are constant at a given temperature and are linear functions of temperature. the d(3)170, d(3)190, d(3)210, d(3)230, and d(3)250 values for e. coli lipopolysaccharide are 251, 99 ... | 1978 | 103502 |
| [serratia in respiratory tract infection (author's transl)]. | | 1978 | 103986 |
| homology of the gene coding for outer membrane lipoprotein within various gram-negative bacteria. | the mrna for a major outer membrane lipoprotein from escherichia coli was found to hybridize specifically with one of the ecori and one of the hindiii restriction endonuclease-generated fragments of total dna from nine bacteria in the family enterobacteriaceae: e. coli, shigella dysenteriae, salmonella typhimurium, citrobacter freundii, klebsiella aerogenes, enterobacter aerogenes, edwardsiella tarda, serratia marcescens, and erwinia amylovora. however, among the enterobacteriaceae, dna from two ... | 1979 | 104972 |
| therapeutic experience with netilmicin. | netilmicin, a new aminoglycoside antibiotic, was used to treat 19 patients with urinary tract infection and 5 with systemic infection. the causal organisms were escherichia coli (in 2), klebsiella pneumoniae (in 4), serratia marcescens (in 12) and pseudomonas aeruginosa (in 7); 1 patient was infected with two of these organisms. all the isolates of causal organisms except one of serratia were initially sensitive to netilmicin but many were resistant to other aminoglycosides. sixteen of the urina ... | 1978 | 105797 |
| defective cellular immunity to gram-negative bacteria in cystic fibrosis patients. | in vitro lymphocyte responses to pseudomonas aeruginosa have been found to be impaired in cystic fibrosis patients with advanced clinical disease. the responses to klebsiella pneumoniae, serratia marcescens, and proteus mirabilis were studied in a similar group of cystic fibrosis patients and normal individuals. cystic fibrosis patients found to be unresponsive to pseudomonas were also unresponsive to klebsiella, serratia, and proteus. responsiveness to staphylococcus aureus was not impaired in ... | 1979 | 106011 |
| disinfection of nitrous oxide inhalation equipment. | cross-infection by contaminated equipment is a potential hazard associated with conscious sedation with nitrous oxide and oxygen . nosocomial infections have occasionally been linked wih the use of unsterile inhalation devices; microbial contamination of sterile nasal hoods routinely occurs during administration of nitrous oxide; and in vitro experiments indicate that subsequent use of contaminated nasal masks may lead to aspiration of microorganisms. although the incidence of respiratory diseas ... | 1979 | 106079 |
| tk polyagglutination associated with reduced a and h activity. | tk polyagglutinable erythrocytes are described in which a and h activities were reduced. it is suggested that bacteroides fragilis isolated from cultures of the patient's blood was responsible for each of these membrane modifications. | 1978 | 106548 |
| cavitary pulmonary lesions complicating use of flow-directed balloon-tipped catheters in two cases. | | 1979 | 106701 |
| hospital isolates of serratia marcescens transferring ampicillin, carbenicillin, and gentamicin resistance to other gram-negative bacteria including pseudomonas aeruginosa. | thirteen independent isolates of serratia marcescens associated with nosocomial urinary tract infections were obtained from the clinical microbiology laboratory at hines veterans administration hospital. the isolates were resistant to at least ampicillin, carbenicillin, gentamicin, and tobramycin. they could be divided into two groups on the basis of their antibiotypes. group i (9 strains) showed resistance to 13 antibiotics, including 3 beta-lactams, 6 aminoglycosides, tetracycline, sulfonamide ... | 1979 | 106772 |
| [intensification of antibacterial and antiviral nonsusceptibility and of the immune response with an officinal rna preparation]. | sodium nucleinate increased essentially the insusceptibility of mice to pathogenic escherichia, strain o26, pr. vulgaris, ps. aeruginosa, ser. marcescens, and produced a total stimulating effect on the nonspecific bacterial resistance; analogous stimulating activity was found in the homologous low polymeric rna from the liver. sodium nucleinate intensified the insusceptibility of the animals to the tick-born encephalitis and encephalomyelitis viruses, and increased the antibody-forming cells cou ... | 1979 | 106911 |
| [comparison of two methods of antibiotic sensitivity test: gel diffusion and automatic method using abac apparatus. ii. study on 399 strains isolated in hospital (author's transl)]. | the authors compared for routine use, the results obtained on 399 strains of gram negative bacilli by two technics of antibiotic sensitivity: the technic of gel diffusion with imprenated discs and an automatic method (abac apparatus). among the antibiotics used as a routine in our laboratory by the diffusion method and the 16 studied with the abac apparatus, 11 are common to both methods and were compared. the concordance of the results was greater than 90% for colistine (94.5%), oxolinic acid ( ... | 1978 | 107836 |
| [investigations on the aminoglycoside inactivating enzyme aac-(6') iv (author's transl)]. | in serratia marcescens and pseudomonas aeruginosa from clinical specimen acetyltransferase aac-(6') iv leads to varying phaenotypically expressed resistance to aminoglycoside antibiotics. the substrate profile and the rate of inactivation of the enzymes investigated are almost identical with each other and with the enzyme of p. aeruginosa gn 315. the enzymes cannot be associated with r factors by the standard methods for conjugation, transformation, density gradient centrifugation and agarose g ... | 1979 | 108221 |
| [effect of exogenous nucleodepolymerases on bacillus subtilis multiplication]. | the effect of exogenous nucleodepolymerases on cell growth was studied with bacillus subtilis. nucleodepolymerases of microbial and animal origin stimulated growth of the culture. the stimulating action of dnaases depended on the dose of the enzyme added to the growth medium and on the phase of the cultural growth. acceleration of the cultural growth correlated with intensification of dna synthesis in the bacterial cells. | 1979 | 108529 |
| pseudomonas aeruginosa vasculitis and bacteremia following conjunctivitis: a simple model of fatal pseudomonas infection in neutropenia. | during attempts to create a realistic model of fatal bacteremia due to pseudomonas aeruginosa during immunosuppression, it was found that the invasive as well as the disseminated phase of infection could be mimicked by gentle instillation of 10(8) colony-forming units of p. aeruginosa into the intact conjunctival sac of agranulocytic rabbits. within 48 hr animals developed conjunctivits leading to severe necrotizing vasculitis and fatal bacteremia. twelve of 26 strains from patients with p. aeru ... | 1979 | 109545 |
| selective proteolysis of the beta 2 subunit of serratia marcescens tryptophan synthase. | | 1979 | 110267 |
| aminopeptidase i activities in several microorganisms. | aminopeptidase i activity which was found to be localized in the same subcellular fraction and to be similarly heat stable was partially purified by a common procedure from escherichia coli b, escherichia coli k12, enterobacter aerogenes, salmonella typhimurium, serratia marcescens pseudomonas aeruginosa, and proteus vulgaris. the enzyme preparations were shown to contain a single animopeptidase active toward both leucylleucine and methionylalanylserine by mixed-substrate initial-velocity kineti ... | 1978 | 110425 |
| gram-negative germs infections in infancy. | the incidence of systemic or local infections due to gram-negative bacilli in an infant ward from september 1969 to december 1976 was 7.9%. the 29.34% were septicemia, most of them as epidemic outbreaks caused by pseudomonas aeruginosa, klebsiella-enterobacter and serratia marcescens. two facts are to be emphasized: an almost complete disappearance of systemic infections with pseudomonas starting from 1972, and the global predominance of the group klebsiella-enterobacter, particularly evident fr ... | 1979 | 112565 |
| antibacterial activity of a new parenteral cephalosporin--hr 756: comparison with cefamandole and ceforanide. | hr 756, a new parenteral cephalosporin that is beta-lactamase resistant, was tested against 271 bacterial isolates. both agar and broth dilution testing were employed, using two media and two inoculum sizes of bacteria. antibacterial activity of the drug was compared to that of cefamandole (cfm) and ceforanide (cfn). in agar, hr 756 was more active than cfm and cfn against all bacteria tested except isolates of staphylococcus aureus, which were better inhibited by cfm. hr 756 exhibited some anti ... | 1979 | 112919 |
| in vitro comparison of rosamicin and erythromycin against urinary tract pathogens. | the in vitro activity of rosamicin and erythromycin was compared at various ph values against 311 strains of bacteria representing common urinary tract pathogens. alkalinization of the media consistently and significantly increased the antibacterial activity of rosamicin against all of the organisms tested. this was also true for erythromycin except when tested against strains of proteus. at ph 8, rosamicin was two- to sixfold more active than erythromycin against enterobacteriaceae. the activit ... | 1979 | 112920 |