| effect of oligomycin on coupling in isolated mitochondria from oligomycin-resistant mutants of saccharomyces cerevisiae carrying an oligomycin-resistant atp phosphohydrolase. | | 1977 | 20055 |
| effects of bromocriptine on adenylate cyclase and phosphodiesterase activities of rat striatum. | the activity of 2-bromo-alpha-ergokryptine (bromocriptine) (5 mg kg-1, i.p.) on adenylate cyclase and on phosphodiesterase (pde-pde ii) of rat striatum, has been examined both in vitro and in vivo. in vitro and in vivo bromocriptine stimulated adenylate cyclase activity, but reduced the stimulating effect of dopamine on adenylate cyclase activity. bromocriptine showed a dose-dependent biphasic action on phosphodiesterases in vitro while in vivo it stimulated them. the results obtained proved bro ... | 1978 | 24111 |
| esterase zymograms of proteus and providencia. | the intracellular esterases of 80 strains of proteus and providencia were analysed by the acrylamide-agarose zymogram technique using several synthetic substrates. the esterase bands were classified in five main groups. the alphaa-esterase bands hydrolysed alpha-naphthyl acetate and were resistant or relatively insensitive to di-isofluoropropyl phosphate (dfp). the alphab-esterase band hydrolysed both alpha-naphthyl acetate and alpha-naphthyl butyrate and were very sensitive to dfp. both groups ... | 1975 | 48538 |
| letter: providencia stuartii infections. | | 1975 | 53535 |
| in vitro activity of amikacin and ten other aminoglycoside antibiotics against gentamicin-resistant bacterial strains. | sixty-nine strains of gentamicin-resistant gram-negative bacilli obtained from different geographical sources were tested for susceptibility to 11 aminoglycoside antibiotics. from the results of determinations of minimal inhibitory concentrations, patterns of resistance were established for 45 strains of enterobacteriaceae and 24 strains of pseudomonas aeruginosa. overall, 81% of the strains were sensitive to amikacin and 33% of the strains were sensitive to butirosin, the next most active compo ... | 1976 | 62815 |
| dietary fibre and experimental colon cancer. | | 1979 | 85018 |
| in vitro synthesis of infectious transforming dna by the avian sarcoma virus reverse transcriptase. | infectious dna molecules, capable of transforming chicken embryo fibroblasts, can be synthesized by the rous sarcoma virus-associated reverse transcriptase in vitro. the optimal enzymatic conditions employed for infectious dna synthesis also facilitate maximum synthesis of genome length dna. analysis of the dna product synthesized by detergent-disrupted rous sarcoma virus under these conditions indicates that dna complementary to viral rna (minus-strand dna) is genome length in size, whereas dna ... | 1979 | 85719 |
| demonstration of an alpha2-macroglobulin receptor in human fibroblasts, absent in tumor-derived cell lines. | | 1979 | 87392 |
| moloney virus-induced cell surface antigens and histocompatibility antigens are located on distinct molecules. | | 1979 | 87439 |
| human corneal endothelium in organ culture. the influence of temperature and medium of incubation. | forty-four human corneas from patients between 21 and 86 years were incubated in eagle's minimum essential medium with earle's salts 10--46 h post mortem. the influence of incubation temperature and composition of the medium on endothelial survival was evaluated. whole corneas were stained by alizarine red. recent cell loss was indicated by morphological alterations in the endothelial pattern. after 20--28 h of incubation minimum cell loss was found at 31 degrees c when 8% dextrane-250 and 20% s ... | 1979 | 88158 |
| the role of p23,30-bearing human macrophages in antigen-induced t lymphocyte responses. | | 1979 | 88270 |
| diagnostic dilemma of a 13-month-old boy with 'late-onset' combined immunodeficiency. | a 13-month-old boy had a "late-onset" form of combined immunodeficiency and a fulminant pneumocystis carinii pneumonia of one month's duration. there was no evidence of cutaneous-delayed hypersensitivity responses to diphtheria-tetanus toxoids, candida albicans, or streptokinase-streptodornase, or of lymphocyte dna synthesis after in vitro stimulation with the mitogens phytohemagglutinin and concanavalin a, and only 2% to 4% of peripheral blood e-rosetted t lymphocytes. the serum igm level was n ... | 1979 | 88898 |
| a new assay of estrogen receptor by thin-layer gel filtration. | | 1979 | 88911 |
| [post-extrasystolic and post-pacing potentiation. i. experimental study of the isolated heart of the rabbit]. | | 1978 | 88947 |
| clinical aspects of alpha 1-fetoprotein determination in human liver cancer and in humans and experimental animals at carcinogenic risk. | recent findings concerning the significance of alpha 1-fetoprotein (afp) as a tool for clinical diagnosis and monitoring of tumor diseases are reviewed briefly. the applicability of this protein marker to the early diagnosis of patients at carcinogenic risk is discussed. in addition, experimental data obtained with a model of chemical hepatocarcinogenesis are reported. the increase of proliferative activity in precancerous liver tissue preceded afp production under experimental conditions with a ... | 1979 | 89199 |
| effect of encephlitogenic protein. ppd and tetanus toxoid on leukocyte migration in agarose. a study of "cross-reactivity". | the reactivity to three antigens: bovine encephalitogenic protein (ep), ppd, and tetanus toxoid, was studied with blood leukocytes from healthy humans using clausen's (5) leukocyte migration in agarose technique. there is an obvious correlation between the reaction of ep (all concentrations studied) and to low concentrations of ppd; and between the reactivity to low concentrations of ep and low concentrations of tetanus toxoid. after vaccination with tetanus vaccine, a marked increase in reactiv ... | 1979 | 89821 |
| modulation of cellular interactions between c3h/10t1/2 cells and their transformed counterparts by phosphodiesterase inhibitors. | it has been demonstrated previously that nontransformed c3h/10t1/2cl8 mouse embryo fibroblasts (10t1/2) can induce a state of reversible growth inhibition in cocultured malignantly transformed mouse fibroblasts and that this inhibition is modulated by serum concentration. the present study suggests that cyclic nucleotides may be implicated in this intercellular communication. the phosphodiesterase inhibitors theophylline, caffeine, and 3-isobutyl-1-methylxanthine (ibx) at concentrations of 10(-3 ... | 1979 | 89899 |
| in vitro studies of the replication of moloney murine leukemia virus. | | 1979 | 90575 |
| cell differentiation in human gastric gland as revealed by nuclear binding of tritiated actinomycin. | the nuclear binding of h3 actinomycin, which is closely linked to the differentiation phenomenon, was studied in human normal gastric mucosa. actinomycin binding decreases in cells which differentiate and becomes very low in fully differentiated cells. in the gastric pits, there is a decreasing gradient of labelling from the deeper stem cells to the well-differentiated superficial cells. this indicates that migration and renewal of the surface epithelium occurs following a 'pipe-line' system. al ... | 1979 | 90638 |
| antigenic determinants in the post-mitochondrial lung fraction. | | 1979 | 90649 |
| ["non-specific cross reacting antigen (nca)", a tracer of human granulocytes and monocytes]. | the non specific cross reacting antigen, or nca, is a normal tissue antigen that cross reacts with cea. it bears a specific antigenic determinant that is absent from cea. immunocytological studies first pointed out that nca, but not cea, is present in alveolar macrophages and polymorphonuclears. further work demonstrated that nca is a marker of granulocytic series: it appears at the stage of promyelocyte and likely is linked to the azurophilic granules. the same antigen is also present in periph ... | 1979 | 92357 |
| nonspecific light loss and intrinsic dna variation problems associated with feulgen dna cytophotometry. | nonspecific light loss by the cell-wall-plus-cytoplasm (cwc) can cause a 50% increase in feulgen absorption units in peanut root-tip nuclei as determined by scanning at 450 nm, whereas this phenomenon is not evident with chicken erythrocytes. a two wavelength scanning method of subtracting nonspecific 450 nm absorption from 550 nm feulgen absorption values eliminated the nonspecific light loss in cwc, however, the two wavelength scanning method is time consuming and somewhat impractical with a r ... | 1979 | 92496 |
| effects of intrathecal and intracerebroventricular injections of substance p on nociception in the rat and mouse [proceedings]. | | 1979 | 93501 |
| antifetal and tumor-specific reactivity in human osteosarcoma. | | 1979 | 94187 |
| comparison of antigenic properties among various strains of treponema hyodysenteriae. | agglutinin-absorption and precipitin-absorption studies demonstrated that three strains of treponema hyodysenteriae isolated from cases of swine dysentery were antigenically different from each other and also from a spirochete isolated from a clinically normal dog. each of the strains of t. hyodysenteriae also possessed two common antigens. | 1979 | 95077 |
| tumor immunology in domestic animals. | | 1979 | 95155 |
| [immunological problems in oncology (author's transl)]. | the authors, after a view of the problems of immunology, regarding the most recent discoveries, deal with the conclusions about the immunological reactions in research and clinical oncology. many specific antibodies, formed as a result of the tumor, have been discovered in the field of research. it is impossible to say the same about human tumors and the probable hypothesis are numerous. the results until now obtained, even if they don't give a definite response, show that the immune defense of ... | 1979 | 95475 |
| [value of the use of a cement containing a combination of triamcinolone and tetracycline in cavity preparations]. | odontomix (reg'd) cement was used at the bottom of cavities prepared in healthy dog canines in a attempt to prevent pulp and dentine alterations caused by the preparation and filling of cavities. 24 dogs were used along with 24 controls. i.v. tetracycline was afterwards given 10 times at weekly intervals. examination of tetracycline labelling under the cavities showed that the cement caused pulp and dentine changes in confined areas, though it has not yet been possible to determine whether these ... | 1978 | 98698 |
| age-associated changes in the blood picture of the goat. | | 1978 | 98936 |
| toxoplasma gondii: penetration into differentiating friend erythroleukemia cells. | | 1978 | 103745 |
| transcription and translation in a pleiotropic streptomycin-resistant mutant of escherichia coli. | the role of the ribosomal protein s12 (streptomycin protein) in ribosome function and in other metabolic processes in the cell has been investigated. a spontaneous streptomycin-resistant strain of escherichia coli (sm3) carrying a mutation in the rpsl gene is deficient in its ability to induce the synthesis of the enzyme bets-galactosidase. it was demonstrated that the reduced rate of enzyme synthesis results from deficiencies in both the transcription of the lactose operon and translation of th ... | 1979 | 104958 |
| isolation of an iron-binding compound from pseudomonas aeruginosa. | an iron-binding compound was isolated from ethyl acetate extracts of culture supernatant fluids of pseudomonas aeruginosa and was purified by successive paper and thin-layer chromatographic procedures. the purified compound was characterized by uv, visible, infrared, and fluorescence spectroscopy. the compound possesses phenolic characteristics, with little or no similarity to dihydroxybenzoates and no indication of a hydroxamate group. p. aeruginosa synthesized the compound during active growth ... | 1979 | 104968 |
| effect of continuously increasing concentrations of plasma ketone bodies on the uptake and oxidation of glucose by muscle in man. | muscle metabolism in man was studied by measuring the arterial and deepvenous concentrations of glucose, lactate, pyruvate, free fatty acids, beta-hydroxybutyrate and aceacetoacetate, and forearm blood flow. after the subjects had fasted overnight, their arterial free fatty acid and ketone levels rose continuously during a period of 90 min, leading to increased ketone body uptake by muscle. hence, for each subject, a relation was obtained between arterial concentrations and arterial-deepvenous d ... | 1978 | 105915 |
| thyroid function studies in the nephrotic syndrome. | total serum and urinary thyroxine (t4), triiodothyronine (t3), and thyroxine-binding globulin (tbg) as well as serum free t4, thyroid-stimulating hormone (tsh), and t3 resin uptake (t3ru) were measured in seven patients with the nephrotic syndrome. the nephrotic syndrome was defined by proteinuria exceeding 3 g/24 h. all patients were clinically euthyroid. most values for total serum t4, free t4, t3, t3ru, tbg, and tsh were within normal limits. however, the mean serum t3 and tbg values were sig ... | 1979 | 106751 |
| trans-stilbene oxide: a new inducer of rat liver microsomal udp-glucuronyltransferase. | | 1979 | 106858 |
| overall pathway of mononucleosome production. | five electrophoretically distinguishable classes of mononucleosomes (mi, mii, ...mv) are produced upon treatment of mammalian nuclear chromatin with micrococcal nuclease. these five forms differ in their initial dna lengths, relative mass proportions, stability, contents of histone h1, and presence of certain nonhistone proteins. a new "chromatin fingerprinting" technique has been developed in order to trace nuclease-mediated interconversions between these mononucleosomes and their polynucleosom ... | 1979 | 107168 |
| [investigations on the aminoglycoside inactivating enzyme aac-(6') iv (author's transl)]. | in serratia marcescens and pseudomonas aeruginosa from clinical specimen acetyltransferase aac-(6') iv leads to varying phaenotypically expressed resistance to aminoglycoside antibiotics. the substrate profile and the rate of inactivation of the enzymes investigated are almost identical with each other and with the enzyme of p. aeruginosa gn 315. the enzymes cannot be associated with r factors by the standard methods for conjugation, transformation, density gradient centrifugation and agarose g ... | 1979 | 108221 |
| [rapid methods useful for determination of streptococcal groups with special reference to streptococci of groups b and d]. | a study was carried out on the efficiency of certain rapid methods for the identification of streptococcal groups, especially groups b and d, methods based upon physiologic properties such as hippurate hydrolysis, production of pigment, esculin hydrolysis, tolerance to salt and bilolysis, associated with the classical tests, for instance the hemolysis type and sensitivity to bacitracin and optochin. by these tests, accessible to hospital laboratories, a number of 3083 streptococcal strains were ... | 1979 | 111335 |
| mutational mosaicism and genetic counseling in retinoblastoma. | mutations may arise throughout an organism's life cycle. typically, sporadic meiotic mutations give rise to individuals with all their germinal and somatic cells bearing the mutant gene. these mutations may be amorphs (with full penetrance and expressivity) or hypomorphs (with reduced penetrance and expressivity). mutational mosaicism, however, involves the origin of mutations occurring during mitosis, whether in the parent at some stage prior to reproductive maturity or in the offspring at some ... | 1979 | 120116 |
| characterization of 5.8s ribosomal ribonucleic acid in neurospora crassa. | neurospora crassa ribosomes contain a species of ribonucleic acid (rna) of molecular weight 54,000, similar to 5.8s ribosomal rna previously described for other eukaryotic organisms. the 5.8s rna from n. crassa was found to be released by heat treatment at 60 c from 25s ribosomal rna but not from 18s ribosomal rna. the base composition of n. crassa 5.8s rna was similar to that of 5.8s rna from saccharomyces cerevisiae, but differed from animal 5.8s rna. during the course of this study, it was di ... | 1975 | 126993 |
| proteoglycans of soluble fraction of mouse mastocytoma. | proteoglycans have been isolated from a high speed supernatant fraction of a mouse mastocytoma by procedures which should minimize alteration of the native protein-polysaccharide molecule. the methods used include in vivo labeling proteoglycans with 35s-sulfate, 3h-leucine and 3h-lysine, centrifugation of the tumor homogenate at 105,000 g, cetylpyridinium fractionation of the supernatant, and further purification of some of the fractions obtained by deae-cellulose column chromatography, gel filt ... | 1975 | 127169 |
| [experiments and theoretical cell kinetic calculations on the problem of in vivo synchronization with vincristine in l 1210 ascites tumor cells and crypt cells of the mouse (author's transl)]. | experiments are described on synchronizing l 1210 ascites tumor cells and jejunal crypt cells of the mouse with vincristine. in both cases the percentage of mitoses increases to a peak value 4 hrs after application of vincristine and decreases during the following 8 hrs ("releasing time") to the level of the normal mitotic index. following this first mitotic peak no futher peak of neither the mitotic nor the labeling index was observed during 48 hrs in l 1210 ascites tumor cells and during 38 hr ... | 1975 | 127443 |
| occupational allergic contact dermatitis to virginiamycin, an antibiotic used as a food additive for pigs and poultry. | a warehouseman aged 31 working in a pharmaceutical factory got an occupational contact dermatitis to virginiamycin (factor m), an antibiotic used as a food additive for pigs and poultry. a review is made of contact dermatitis to virginiamycin and to pristinamycin. | 1978 | 149638 |
| immunological methods employed in an attempt to induce erythema nodosum leprosum (enl) in mice. | in the mouse foot pad model five different parameters were employed to simulate the condition of enl as observed in the human. the experimental groups with five to six months leprosy infection were injected intravenously with various anti-mycobacterial antibodies, m. leprae sonicate and 'b' cells obtained from syngenic donors. the control group of animals, infected similarly, were treated either with m. leprae sonicate or gamma globulins precipitated from normal human serum. all recipients were ... | 1978 | 151764 |
| irs, hew threaten triple-a bonds. | | 1978 | 152403 |
| effects of sarcoplasmic reticulum ca2+-atpase on phospholipid bilayer fluidity: boundary lipid. | | 1978 | 153147 |
| changes in somatostatin concentration in pancreas and other tissues of streptozotocin diabetic rats. | changes in immunoreactive somatostatin were examined in islets, whole pancreas, stomach, and hypothalamus of streptozotocin-diabetic rats. there was no change in islet somatostatin content at 2 days after the administration of streptozotocin, but thereafter, somatostatin progressively increased in the diabetic animals by 45% at 2 weeks, 230% at 6 weeks, and 500% by 6 months. by contrast, islet glucagon rose acutely and maintained a constant 2-fold elevation irrespective of the duration of the di ... | 1978 | 154402 |
| experimental evaluation of a posterior chamber lens fixated with dacron. | a posterior chamber intraocular lens using dacron fibers to achieve fixation to the iris was studied. the lens was inserted in rabbit eyes following extracapsular lensectomy, and in primate eyes after lensectomy, vitrectomy, and peripheral iridectomy. surgical implantation of the lenses was simple and quick, and no operative complications occurred. clinically, no difference was noted in the amount of inflammation in eyes receiving a lens with or without dacron. none of the lenses implanted in pr ... | 1979 | 154637 |
| mutations altering genetic recombination and repair of dna in bacteriophage t4. | | 1975 | 163533 |
| [immunologic results obtained with an experimental system oftransplantable colonic tumors. possible applications of these results to human intestinal tumors]. | intestinal cancers, morphologically very close to human colo-rectal adeno-carcinoma, have been induced by dimethylhydrazine in inbred rats. graftable lines have been obtained from 5 primary intestinal tumors, and 3 cell lines have been cultivated from the grafts. this model was used to demonstrate carcinofetal antigen(s) on the surface of the intestinal cancer cells. circulating antibodies against tumor-associated antigen(s) have been found in tumor-bearing rats. cancer enhancement was regularly ... | 1975 | 174767 |
| multiple molecular forms of lactate dehydrogenase and glucose 6-phosphate dehydrogenase in normal and abnormal human breast tissues. | normal and abnormal breast samples of women were analyzed for multiple molecular forms of lactate dehydrogenase and glucose 6-phosphate dehydrogenase, using acrylamide disc gel electrophoresis and specific enzyme staining techniques for separation and quantitation. infiltrating ductal carcinomas demonstrated a significant increase (three to six-fold) in the proportion of ldh-5 compared to samples of normal breast, fibrocystic disease and fibroadenoma, indicative of a shift toward the muscle-type ... | 1976 | 177177 |
| radioimmunoassay of 17beta-hydroxy-5alpha-androstan-3-one, 4-androstene-3,17-dione, dehydroepiandrosterone, 17-hydroxyprogesterone and progesterone and its application to the human male plasma. | | 1976 | 178963 |
| [use of the immunofluorescence test as a rapid diagnostic technic in influenza]. | | 1975 | 179228 |
| the cleavage of polyoma virus dna by restriction enzymes kpni and psti. | the action of two restriction endonucleases on polyoma virus dna has been examined and the sites at which they cleave the dna located. one of the enzymes, kpni from klebsiella pneumoniae ok8, cleaves polyoma dna twice at about 11-6 and 59-2% from the ecori site. the other enzyme, psti from providencia stuartii 164, cleaves polyoma dna five times at about 14-8, 16-5, 32-6, 50-3 and 80-0% from the ecori site. some of the cleavages produced by these enzymes alone, or in conjunction with other endon ... | 1976 | 180247 |
| a protein-binding assay for direct determination of adenosine 3',5'-monophosphate in amniotic fluid, cerebrospinal fluid, plasma, and urine. | an adenosine 3',5'-monophosphate (cyclic amp)-binding protein was isolated from bovine skeletal muscle. this preparation showed maximum binding capacity for cyclic amp at the physiological ph of amniotic fluid, cerebrospinal fluid, and plasma and had a high association constant of 1.4x10(9) 1/mol. this preparation of binding protein, together with albumin and edta in the assay buffer, gave a sensitive and specific competitive protein-binding assay that permitted direct determination of cyclic am ... | 1976 | 181838 |
| virus-induced murine leukemia involving two subpopulations of lymphocytes. | | 1975 | 183714 |
| phosphorylated sites of calf thymus h2b histone by adenosine 3':5'-monophosphate-dependent protein kinase from bovine cerebellum. | | 1976 | 183776 |
| identification and discharge patterns of spinal sympathetic interneurons. | sympathetic interneurons, in the vicinity of the intermediolateral cell column of the cat thoracic spinal cord, were identified by determining whether the probability of spontaneously occurring unitary discharge was correlated in time with the r wave of the ecg. fifteen units which could not be antidromically activated by stimulation of the cervical sympathetic nerve exhivited a positive post-r wave relationship. the discharge patterns of these cells were distinctly different from those of antid ... | 1976 | 184713 |
| markers to distinguish normal and neoplastic mammary epithelial cells in vitro: comparison of saturation density, morphology and concanavalin a reactivity. | normal and premalignant mouse mammary epithelial cells can be prepared in high yields by collagenase dissociation of minced glands followed by a brief, differential centrifugation to remove contaminating fibroblasts and fat cells. the major difficulties in preparing pure cultures in quantity are 1) incomplete dissociation of gland material, and 2) cell death during enzymatic digestion. these problems are eliminated by careful selection of collagenases for dissociation. normal and premalignant ma ... | 1976 | 185159 |
| opiate receptor: autoradiographic localization in rat brain. | opiate receptor sites in rat brain can be labeled in vivo by [3h]diprenorphine, a potent opiate antagoinst. using techniques to minimize diffusion in fresh, frozen, unfixed brain, we have localized [3h]diprenorphine by autoradiography to visualize the distribution of opiate receptors. silver grains indicative of the binding of labeled [3h]diprenorphine are discretely localized in numerous areas of the brain with very high densities in the locus coeruleus, the substantia gelatinosa of the spinal ... | 1976 | 185626 |
| subcellular distribution of steroids in rat adrenocortical tissue after incubation in vitro [proceedings]. | | 1977 | 194992 |
| the influence of metabolities on the assay of platelet serotonin. | | 1977 | 196357 |
| viral hepatitis: enzyme assays and serologic procedures in the study of an epidemic. | an epidemic of viral hepatitis beginning in late 1975 in a residence for multiply handicapped children, recognized very early in its course, was investigated prospectively to permit comparison of enzymatic and serologic tests. thirty-three residents of the institution and 46 full- and part-time employees were studied by the immune adherence hemagglutination procedure for antibody (anti-hav) to hepatitis a virus (hav). of these, 31 residents and 37 staff members were susceptible at the beginning ... | 1977 | 201170 |
| human leukocyte migration inhibitory factor (lif). iv. 3',5'-cgmp protects lif against inactivation by the esterase inhibitor phenylmethylsulfonyl fluoride. | recently reported experiments suggest that human leukocyte migration inhibitory factor (lif) has properties of an esterase and a protease with substrate specificities directed against arginine esters and amides. also reported previously, the synthetic phosphodiester bis-p-nitrophenyl phosphate (bnpp) but not various phosphomonoesters preserve lif activity in the presence of the serine esterase inhibitor phenylmethylsulfonyl fluoride (pmsf). in this paper i demonstrate that guanosine 3'5'-cyclic ... | 1977 | 203999 |
| paradoxical effect of mecillinam on providencia stuartii. | | 1976 | 206531 |
| location of the cleavage sites on the sv 40 dna map produced by the restriction endonucleases pst 1 and bam 1. | restriction endonucleases from providencia stuartii (pst 1) and bacillus amyloliquefaciens h (bam 1) cleave sv 40 dna at two and one specific sites, respectively. using ecori and hind iii endonuclease restriction sites as reference, the two pst i sites were mapped at 0.050; 0.265 and the bam i site was mapped at 0.170 of the genome length, clockwise, from the single ecori cleavage site. | 1978 | 210371 |
| clinicopathologic features of primary and metastatic neoplastic disease of the liver in dogs. | | 1978 | 211107 |
| transfer of duck cell dna sequences to the nucleus of 3t3 cells by rous sarcoma virus. | peking duck cell nuclear dna has no complementarity to rna of prague c rous sarcoma virus (rsv). upon infection of peking duck cells by prague c rsv, polynucleotide sequences complementary to peking duck cell nuclear dna can be detected in the high molecular weight rna from progeny peking duck cell passaged rsv (rsvpd) by rna.dna molecular hybridazation. when 3t3 cells are subsequently infected by rsvpd, polynucleotide sequences complementary to peking duck cell nuclear dna can be detected in th ... | 1978 | 212741 |
| adenosine kinase from rabbit liver. i. purification by affinity chromatography and properties. | | 1979 | 218933 |
| isolation of foot-and-mouth disease virus messenger rna from membrane-bound polyribosomes and characterization of its 5' and 3' termini. | | 1979 | 228483 |
| hr 756, a highly active cephalosporin: comparison with cefazolin and carbenicillin. | hr 756, a new parenteral cephalosporin, was compared with cefazolin and carbenicillin for activity against a total of 264 strains of pseudomonas aeruginosa, escherichia coli, klebsiella spp., proteus mirabilis, proteus spp. (indole positive), enterobacter spp., salmonella typhi, serratia marcescens, providencia stuartii, and staphylococcus aureus. in every comparison, except that with the last organism, hr 756 was clearly more active than cefazolin and carbenicillin. all three compounds had simi ... | 1978 | 253572 |
| haloxon as an anthelmintic for dogs and cats. | | 1977 | 272801 |
| relationship between fluoride in the drinking water and the plasma fluoride concentration in man. | | 1978 | 272948 |
| prospects of prevention of recurrent herpetic eye disease. | various theories of recurrence of herpetic disease are outlined to indicate the potential role that interferon, interferon-inducers, or a hypothetical perfect synthetic antiviral may have in preventing recurrences, and the possibilities of minimizing trigger effects are considered. attention is drawn to certain aspects of the management of herpetic eye disease that have a direct bearing on the incidence of subsequent recurrence. there is a need to initiate a movement to mobibize ophthalmologists ... | 1977 | 273346 |
| pregnancy-specific beta-1 glycoprotein (sp-1) in normal and abnormal pregnancy. | maternal serum levels of a pregnancy specific beta-1 glycoprotein (sp-1) were measured by radial-immunodiffusion in 369 normal pregnancies. mean levels rose progressively to approximately 200 mg/l at 36 weeks of gestation followed by a plateau and a fall at term. the 95% confidence limits were established for sp-1 logarithmic correction of the positively skewed raw data and certain theoretical and practical advantages were demonstrated in the use of sp-1 compared with human placental lactogen (h ... | 1978 | 283780 |
| the proportional volume of mucous acinar cells in normal human submandibular salivary glands. | | 1979 | 294238 |
| a simple one-step hemolytic assay for c2 with c2-deficient human serum. | a simple one-step procedure has been developed for the molecular titration of c2 by utilizing the ability of the test material to restore the hemolytic activity of human serum selectively deficient in c2 (c2d serum). in this assay, equal volumes of ea (10(8) cells/ml), c2d serum (1/20), and a suitable dilution of a source of c2 were incubated at 37 degrees c for 60 min and the fraction of cells lysed was used to calculate the effective molecules of c2/ml test material. the assay can be used to t ... | 1977 | 321681 |
| therapy for experimental intraabdominal sepsis: comparison of four cephalosporins with clindamycin plus gentamicin. | an experimental model of intraabdominal sepsis in rats was used for evaluation of treatment with four cephalosporins: cephalothin, cefazolin, cefoxitin, and cefamandole. doses were 115 mg of each of the cephalosporins/kg and, in additional experiments, 350 mg of cephalothin or cefazolin/kg. response to treatment was evaluated by mortality rate and the incidence of intraabdominal abscess. all four cephalosporins and gentamicin produced a similar reduction in mortality rates. there was a marked va ... | 1977 | 321707 |
| in vitro susceptibility of cephalothin-resistant enterobacteriaceae to cefoxitin and bl-s786. | the in vitro antibacterial activity of two agents relatively resistant to beta-lactamases, bl-s786 and cefoxitin, was tested against 123 recent different clinical isolates of cephalothin-resistant enterobacteriaceae. bl-s786 showed considerable activity against escherichia coli and lesser activity against klebsiella pneumoniae with, respectively, 68% and 41% inhibited at 32 microng/ml. cefoxitin showed more activity in vitro against e. coli, k. pneumoniae, serratia marcescens and providencia stu ... | 1977 | 324961 |
| unusual enterobacteriaceae. "proteus rettgeri" that "change" into providencia stuartii. | a blood culture bottle from a patient with bacteremia contained both proteus rettgeri biogroup 5 and providencia stuartii (described in bergey's manual of determinative bactiology [8th ed., 1974] as proteus inconstans), which had the same unusual antibiotic resistance pattern. single colonies of this p. rettgeri biogroup 5 isolate were shown to produce urea- clones. if current taxonomy is used, the strain changed from p. rettgeri to p. stuartii in the laboratory and probably also in the patient. ... | 1977 | 334793 |
| aminoglycoside inactivating enzymes produced by r plasmids of escherichia coli, citrobacter freundii, klebsiella pneumoniae, proteus vulgaris, providencia stuartii and serratia marcescens. | | 1977 | 340445 |
| rapid tween 80 hydrolysis test for mycobacteria. | a rapid tween 80 hydrolysis test for mycobacteria utilizing gas-liquid chromatography is described. the test requires 1 h of incubation followed by simple extraction and chromatography of nonderivatized oleic acid. | 1978 | 342536 |
| inhibition of thymidine phosphorylase in vivo provides a rapid method for switching dna labeling. | uridine blocks the in vivo conversion of thymine to thymidine in escherichia coli, thus, one can change dna labels by labelling first with a thymine label (e.g. 14c) and then, at the time of the change, adding 50 microgram uridine per ml and thymidine (e.g. 3h). the cells immediately start using the thymidine, ignore the thymine for several generations, and are not affected by the uridine. | 1977 | 342905 |
| preparation of acid-fast microscopy smears for proficiency testing and quality control. | a method is presented for preparing smears for proficiency testing and quality control in acid-fast microscopy. the work was prompted by the increased demand for acid-fast bacilli positive smears with characteristic microscopic appearance and among-smear uniformity. | 1978 | 353070 |
| simultaneous production of two types of beta-lactamase in escherichia coli and providencia stuartii. | the production of beta-lactamase has been studied in two strains isolated from clinical samples: providencia stuartii mulb 501 and escherichia coli mulb 130. these strains were selected for their high resistance level to penicillins and cephalosporins. determination and identification of beta-lactamase activity were achieved by combining several up-to-date methods including (i) neutralization by anti-beta-lactamase sera, (ii) purification by affinity chromatography on cephalosporin c linked seph ... | 1978 | 365308 |
| [investigation on the presence of salmonella in drinking water from water supplies and distribution systems in togo (author's transl)]. | bacteriological drinking water examination of samples from water works and wells as well as from their distribution systems revealed that from 26 samples, where salmonella organisms could be isolated, in 16 cases e. coli- and coliforms could not be found and that in 9 of these cases even the colony count was below 100 colonies per ml at 20 degrees c and 37 degrees c incubation temperature. the common reasons for such contaminations are technical deficiencies, mostly faulty well-heads or broken c ... | 1979 | 384726 |
| comparative bactericidal effect of ceforanide (bl-s 786) and five other cephalosporins in an in vitro pharmacokinetic model. | the bactericidal activity of ceforanide was compared, in an in vitro kinetic model, with that of five other cephalosproins: cephalothin, cefazolin, cefamandole, cefuroxime, and cefoxitin. cultures of various pathogens in 95% human serum were incubated for 12 hours in the presence of the cephalosporins whose concentrations were modified periodically-by addition of a concentrated solution of drug or dilution with unmedicated serum-in order to simulate the variation of antibiotic concentration in h ... | 1979 | 396292 |
| [use of a laser photometer to determine blood platelet shape and aggregation in a flowing system]. | the paper treats of an original device for studies with the photometric method of the platelets shape and of their aggregation and disaggregation processes in the flow. experiments on the stenosed vessel model showed that in the presence of adp stenosis potentiated the platelets aggregation in the rabbit platelet-rich plasma. | 1979 | 420923 |
| o serotyping of providencia stuartii isolates collected from twelve hospitals. | a collection of 829 isolates of providencia stuartii, mostly from urological specimens of patients in 12 hospitals, were o serotyped. hospitals varied in serotype distribution, but most isolates (97%) fell into one or another of 14 o types of p. stuartii. one type (o63) was found in 10 hospitals, and six types (o4, o17, o25, o52, o55, o56) were found in 5 or more hospitals. these seven types were more common than others and included 753 (91%) of the isolates. only four isolates agglutinated in p ... | 1979 | 429535 |
| liver abscess caused by haemophilus paraphrohaemolyticus. | haemophilus paraphrohaemolyticus was isolated in pure culture from a liver abscess in a 73-year-old man. | 1979 | 429547 |
| culture of human haemopoietic cells in diffusion chambers. i. growth of normal peripheral blood cells. | growth of mononuclear cells from human peripheral blood from 10 normal individuals was tested in diffusion chamber culture over a period up to 17 or 21 days. after an initial decrease during the first few days an increase of the total cell number was observed with maximal values on day 13. in all individuals growth of undifferentiated blast cells, lymphocytes, plasma cells, immature and mature granulopoietic cells, macrophages, and megakaryocytes occurred. in all individuals the different cell t ... | 1979 | 444678 |
| [nosocomial infections with providencia stuartii (proteus inconstans) (author's transl)]. | 48% of all isolates of providencia stuartii obtained within a period of one year, originated from the urine of catheterised patients, whereas another 37% were found in the respiratory tract of patients with intubation. the incidence of providenica stuartii correlated with the period during which iatrogenic measures had been applied, as well as with a preceding course with antibiotics. | 1979 | 467264 |
| [dynamics of intramembrane particles during proteolysis of certain membranes]. | freeze-fracture electron microscopy has been used to study the ultrastructure of proteolytic enzymes treated of the bovine photoreceptor membranes, the rat liver microsome ghosts and the rabbit sarcoplasmic reticulum membranes. the observed increase in the intramembranous particle number in the inner fracture face suggests transmembrane dipping of amphipathic integral proteins affected by the partial proteolysis. | 1979 | 476184 |
| an xyy man. | this case report describes a young man with a 47, xyy karyotype who was convicted of arson. he suffered from a cardiac disorder which may well have been part of the xyy syndrome rather than a chance association. his abnormal karyotype was disclosed in court and used by the defence in a plea in mitigation. | 1979 | 497632 |
| the fibrinolytic activity of the vein following venous stasis. | this animal study investigated the fibrinolytic activity in the vein wall of the pig following venous stasis, which was induced artificially using an intermittently inflated pneumatic cuff. the method used to measure the fibrinolytic activity was modified from todd's original technique. the area of lysis and the area of the vein wall were measured and expressed as a ratio, called the fibrinolytic index. after 3 h of intermittent venous stasis the fibrinolytic index was reduced by 68 per cent, si ... | 1979 | 497652 |
| [effect of indolylacetic acid on formation of bacteroid forms of rhizobium leguminosarum]. | the purpose of this work was to study the effect of indolylacetic acid (iaa) on the strains of rhizobium leguminosarum, effective and noneffective with respect to symbiotic nitrogen fixation (l4 and 245a, and 14--73, respectively). iaa at a concentration of 50 mcg/ml and higher inhibited the growth of the bacterium, temporarily delayed celular division, and induced intensive formation of elongated bacteroid-like cells, predominantly y-shaped or having a clavate shape. many bacteroid-like cells w ... | 1979 | 502911 |
| spinal analgesia and sedation of goats with lignocaine and xylazine. | spinal anaesthesia of 20 does undergoing laparotomies was accomplished with epidural injections of 2 per cent lignocaine at a dosage of 1 ml per 4.55 kg in 16 goats. epidural injection resulted in paralysis lasting 198.5 minutes +/- 36.6 minutes. subarachnlid injection resulted in paralysis lasting 180.8 minutes +/- 44.9 minutes. xylazine was given intramuscularly at the dosage of 0.11 mg per kg after posterior paralysis was complete. these treatments resulted in effective analgesia for surgical ... | 1979 | 516312 |
| o-serotyping providencia alcalifaciens. | the o-serotyping scheme for providencia was tested on providencia alcalifaciens isolates collected mostly from two hospitals. the specificites of the somatic (o) antigens of p. alcalifaciens were found to be different from those of providencia stuartii, and separation of the providencia typing scheme to allow separate typing of each species led to more efficient typing. all but 4 of 86 isolates were typable. eighteen serotypes occurred among 53 typable isolates obtained from a pediatric hospital ... | 1979 | 521478 |
| application of o-serotyping in a study of providencia rettgeri (proteus rettgeri) isolated from human and nonhuman sources. | a somatic (o) antigen serotyping scheme for providencia rettgeri (proteus rettgeri) was modified to exclude o-type strains recently reclassified as urea-positive providencia stuartii and was extended to include new serotypes to provide for serotyping on the basis of 93 o-antigens. isolates from two hospitals, five public health laboratories, and nonhuman sources (polluted water and frogs) were serotyped. the 112 isolates collected from a large general hospital over a 99-month period were distrib ... | 1979 | 521481 |
| interactions of folch-lees proteolipid apoprotein with planar lipid bilayers. | water-soluble folch-lees proteolipid apoprotein from bovine cns white matter induces a voltage-dependent conductance in black lipid membranes. na+ is required for the induced conductance change but the established conductance has very low ionic selectivity. the induced conductance fluctuates with a minimum amplitude of 10(-11)--10(-10) mho. the magnitude of the conductivity change is dependent on protein concentration and on the composition of lipid bilayers. at a fixed voltage the induced condu ... | 1979 | 522128 |