d-alanyl-d-alanine carboxypeptidase in the bacterial form and l-form of proteus mirabilis.membranes of the bacterial form and the stable and unstable l-forms of proteus mirabilis contain ld and dd-carboxypeptidase. the dd-carboxypeptidase is inhibited non-competitively by penicillin g. the enzyme of the bacterial form is highly penicillin-sensitive (ki - 4 x 10(-9) m penicillin g). inhibition is only partly reversible by treatment with penicillinase or by dialysis against buffer. in contrast, the dd-carboxypeptidase of the unstable l-form, grown in the presence of penicillin, is 175- ...1975238
proceedings: inducible tetracycline resistance in r+ and r- strains of proteus mirabilis. 19752719
bactericidal activity and pharmacology of flucloxacillin.flucloxacillin, a recent addition to the group of isoxazolyl penicillins, was studied in vitro and in normal volunteers. the bactericidal activity of the drug against most strains of gram-positive bacteria including penicillin-resistant staphylococcus aureus was similar to that of oxacillin and approximately fourfold greater than that of cloxacillin. each of the three penicillins was administered orally to a group of ten volunteers for eight days in a dose of 500 mg four times a day. the mean co ...19763969
further observations on the potentiation of the antibacterial effect of methenamine by acetohydroxamic acid.the use of methenamine in the treatment of urinary tract infections due to proteus species is limited by urine alkalinity. acetohydroxamic acid, an inhibitor of urease, maintains acidity despite growth of proteus in urine. easily achievable concentrations of acetohydroxamic acid in vitro systems that simulated the dynamics of the urinary tract potentiated the antibacterial effect of methenamine against proteus species. the combined use of a urease inhibitor and methenamine may be effective in th ...19764573
studies of introital colonization in women with recurrent urinary infections. v. the inhibitory activity of normal vaginal fluid on proteus mirabilis and pseudomonas aeruginosa.normal vaginal fluid from premenopausal volunteers was inoculated with 10 strains of proteus mirabilis and 14 strains of pseudomonas aeruginosa at ph's of 4.3, 4.6 and 4.9. all bacteria were killed at ph 4.3. nine of 10 strains of proteus mirabilis and 12 of 14 pseudomonas aeruginosa were killed at ph 4.6. only 4 of 14 strains of pseudomonas aeruginosa were killed at ph 4.9, while 8 of 10 strains of proteus mirabilis were killed at the same ph. we conclude that in comparison to the common 0 grou ...19764631
comparative in vitro activity of cephalosporins.the in vitro activity of cephalexin, cephaloridine, cephalothin, cephapirin, cefoxitin, cephamycin c, cepharadine and cefazolin was determined against 443 isolates of bacteria. at a concentration of 12.5 mug/ml, all of the cephalosporins inhibited more than 60% of the isolates of klebsiella pneumoniae. at the same concentration, cephalexin, cephaloridine, cephalothin, cephapirin, cephamycin c and cefazolin inhibited more than 90% of isolates of proteus mirabilis. all of the cephalosporins except ...19766411
n-nitrosamine formation by cultures of several microorganisms.of 38 pure cultures of microorganisms tested, only one, pseudomonas stutzeri, was capable of forming dimethylnitrosamine from dimethylamine and nitrite during growth. resting cells of p. stutzeri, cryptococcus terreus, escherichia coli, and xanthomonas campestris formed dimethylnitrosamine, although no nitrosamine was found in growing cultures of the latter three organisms. no nitrosamine was produced by either growing cultures or resting-cell suspensions of pseudomonas fragi or proteus mirabili ...19767197
bactericidal mechanisms in rabbit alveolar macrophages: evidence against peroxidase and hydrogen peroxide bactericidal mechanisms.the role of peroxidase-mediated bacterial killing by rabbit alveolar macrophages was examined. during 3 h of incubation in vitro, alveolar macrophages ingested and killed greater than 88% of the streptococcus faecalis, proteus mirabilis, or streptococcus pneumoniae present in the incubation mixture. preincubation of alveolar macrophages with inhibitors of catalase, 3-amino-1,2,4-triazole or sodium nitrite, did not alter their bactericidal potential. iodination of ingested zymosan particles, a pe ...19767533
factors influencing the immune enhancement of intrapulmonary bactericidal mechanisms.the effect of specific immunization on the antibacterial defense mechanisms of the murine lung was assessed against streptococcus pneumoniae, staphylococcus aureus, staphylococcus aureus (smith), serratia marcescens, klebsiella pneumoniae, proteus mirabilis, and pseudomonas aeruginosa. immunization by aerosol inhalation significantly enhanced the intrapulmonary killing of pseudomonas aeruginosa and proteus mirabilis but not the remaining organisms. with p. mirabilis, systemic immunization induce ...19769364
effect of organic mercurials and sulfhydryl compounds on the urease activity of proteus: inhibition by urine and ascorbic acid.meralluride, mercaptomerin, ethacrynic acid, and penicillamine inhibited urease activity of proteus mirabilis. the activity of the organic mercurials and ethacrynic acid was markedly inhibited by human and dog urine. antiurease activity could not be detected in the urine of a human and a dog given meralluride by injection. urine from patients receiving penicillamine also failed to inhibit urease activity. ascorbic acid inhibited, whereas dehydroascorbic acid enhanced, the activity of the mercuri ...197610828
approach to a practical method for screening and identifying microorganism genera from urine (author's transl).in this study the author reported upon a practical new system for screening and identifying the microbial agents causing urinary tract infections. this system is composed of a combination of 3 screening procedures (ph-value + nitrite-test + catalase-test) and 8 selective culture media for the purpose of genus identification within 24 hours (uripret-g). a total of 130 cultures was investigated. the employed microorganisms were mainly recovered from urine samples. they included the following speci ...197611179
which gentamicin assay method is the most practicable? 197714915
intracellular nucleodepolymerase of bacteria, representatives of the proteus and providencia groups. its isolation and properties.high activity of enzymes, splitting native, denaturated dna, deoxyribooligonucleotides and rna, was observed in cell free extracts of bacteria--representatives of 5 strains of proteus-providencia. some properties of nucleases were studied in cell free extracts. in the bacteria studied dnaases were thermolabile proteins, which were completely inactivated at 50-60 degrees, rnaases were more thermostable. the ph optima of the dnaases were at ph 9.0-11.0 in cell free system; rnaases were maximally a ...197617219
cyclic guanosine-3',5'-monophosphate--mediator of the action of the hormaone erythropoietin. 197718327
differential susceptibility of escherichia coli and proteus mirabilis to mouse urine and to urea.the purpose of this investigation was to compare the antimicrobial properties of mouse urine and of urea against escherichia coli and proteus mirabilis. nornal urine was found to inhibit the growth of e. coli and p. mirabilis, whereas urine from diuresing animals permitted multiplication of these bacteria. addition of urea to urine from diuresing animals restored its bactericidal effect on p. mirabilis but not on e. coli. this bactericidal effect on p. mirabilis was dependent on the additive act ...197718546
the effect of nutrient depletion on the drug resistance of a polymyxin-sensitive proteus mirabilis proceedings. 197722714
factors influencing the therapy of experimental proteus mirabilis meningitis in rabbits.the therapy of proteus mirabilis meningitis with gentamicin alone and in combination with chloramphenicol was studied in a rabbit model. antibiotics were administered for 8 hr. samples of serum and cerebrospinal fluid (csf) obtained simultaneously were assayed at 2-hr intervals for antibiotic concentration and counts of bacteria in csf. the percentage of penetration (concentration in csf divided by concentration in serum x 100%) of gentamicin ranged from 14% to 23%, but very large dosages were r ...197824668
significance of microbial contamination of stored cadaver kidneys.the importance of microbial contamination of cadaver kidneys was assessed in 83 consecutively stored and transplanted kidneys. fourteen kidneys had a single positive culture during storage and five had multiple positive cultures. only one postoperative infection could be traced to kidney contamination during storage (candida wound infection). in three of 64 patients who received noncontaminated kidneys, posttransplant wound infections developed. no wound infections occurred in 35 patients who re ...197825059
purification of dna polymerase from proteus mirabilis. 197825512
the immunotropic effect of cysteine in the foetal period and in adult rats after administration of thymus-independent antigens. 197725698
modification of the ph-auxostat culture method for the mass cultivation of bacteria. 197826435
dipeptidylaminopeptidase and carboxypeptidase activities of the brush border of rabbit small intestine. 197830675
the antimicrobial activity of urine of paraplegic patients receiving methenamine mandelate.the antimicrobial activity of urine collected from adult male paraplegics ingesting methenamine mandelate (mm) was evaluated. the in vitro bacterial growth in urine from these patients was inhibited when the free formaldehyde (hcho) concentration was 10 to 22 microgram per ml. when the hcho concentration was in the region of 28 microgram per ml or greater, bactericidal effect became apparent. urine containing 1323 microgram of mm per ml with a ph of 5.9 when freshly voided had sufficient hcho to ...197830734
the role of thiamine in nervous tissue.the possibility that thiamine (vitamin b1) has a role in nervous tissue that is independent of its well-documented coenzyme function is discussed. after reviewing the localization and metabolism of the vitamin and its phosphate esters, the effects of either thiamine deprivation or antimetabolites of thiamine on conduction and transmission, and the relationship between thiamine triphosphate and the genetic, neurological disease, subacute necrotizing encephalomyelopathy (leigh's disease), it is su ...197937452
microbiological activity of cefazedone as compared to cefazolin and a comparison of the antibacterial activity of (6r,7r)-7-(2-[3,5-dichloro-4-oxo-1(4h)-pyridyl]-acetamido)-3-([5-methyl-1,3,4-thiadiazol-2-yl)-thio]methyl)-8-oxo-5-thia-1-azabicyclo[4,2,0]oct-2-ene-2-carboxylic acid (cefazedone, refosporen) as compared to other cephalosporins it can be seen that cefazedone possesses good activity against gram-positive bacteria; as regards activity against enterobacteriaceae, in particular strains of e. coli, klebsiella species and proteus mirabilis, they fall w ...197939575
ultraviolet irradiation disrupts somatic pili structure and function.three piliated bacterial species were exposed to ultraviolet light (7 x 10(3) microw/cm2), and the effect of increasing duration of irradiation on the integrity of the somatic pili was quantitated by negative-stain electron microscopy. heavily piliated proteus mirabilis became devoid of pili after 20 min of irradiation, but escherichia coli and neisseria gonorrhoeae required 40 min for complete depiliation. partially purified proteus pili underwent progressive loss of structural integrity with i ...197940875
[photodynamic effect of quinacrine on bacteria].the acridine dye quinacrine (qa) was tested with regard to the photodynamic action on bacteria (proteus mirabilis, escherichia coli). the absorption maximum of the yellow dye qa ist in contrary to the photodynamically active dyes methylene blue (mb) and thiopyronine (tp) situated in the short wave region of the visible spectrum. using for illumination a common light source--they have in general a weak emission in the short wave region--relatively high concentrations of qa are necessary for photo ...197942229
[comparison of four recently introduced cephalosporins with respect to probability of resistance in multiresistant strains of escherichia coli, proteus mirabilis and klebsiella spec. (author's transl)].strains with the same number of resistances were arranged in so-called resistance classes. nine classes of resistance (0 to greater to or equal to 8) were formed by means of ten standard chemotherapeutics; the four new cephalosporins were excluded. for every resistance class frequency of cephalosporinresistance was described as coefficient ranging from 0 to 1 (fig. 1). in cephalothin the coefficients were markedly rising only in 6 (7)-fold resistant strains of the species examined. similar but s ...197944785
[the distribution of minimal inhibitory concentrations of recently introduced cephalosporins in multiresistant strains of escherichia coli, proteus mirabilis and klebsiella spec. as revealed by zone sizes of a standardized agar diffusion test (author's transl)].strains of a species were divided into two groups according to the number of resistances (less than or equal to 4, greater than or equal to 5) using 10 standard chemotherapeutics regularly examined, the new cephalosporins not being among them. these groups of less than or equal to 4- and greater than or equal to 5-fold resistant strains were compared for each cephalosporin tested (fig. 1). the most different distributions of zone diameters (of both groups) were seen in cephalothin, whereas in ce ...197944786
incompatibility of r plasmids from clinical material.thirteen r plasmids derived from strains of e enterobacteriaceae isolated from clinical material have been characterized. they belonged to many incompatibility groups and differ widely respecting other phenotypic characteristics, even if they come from bacteria isolated from a small geographic area.197944944
[in vitro activity of cefaclor (author's transl)].a comparative study was conducted on the in vitro activity of cefaclor and other oral cephalosporins against a large number of freshly isolated clinical strains of gram-negative and gram-positive bacteria. the activity of cefaclor against gram-positive pathogens is very similar to that of cephalexin. the action of cefaclor against streptococcus pneumoniae is superior. cefaclor is the most active antibiotic against strains of haemophilus influenzae, and is also more active than cephalexin and cep ...197945087
letter: antagonism of oxolinic acid by nitrofurantoin. 197548654
infection caused by proteus mirabilis strains with transferrable gentamicin-resistance factors.during a period of 10 weeks, four patients in one hospital became infected with gentamicin-resistant proteus mirabilis. in two of them septicaemia associated with indwelling catheters developed, one had urinary tract and wound infections, and in the fourth patient the organism was isolated from a superficial wound. the p. mirabilis strains showed multiple drug resistance. strains form the first three patients were apparently identical and were sensitive to tobramycin. their gentamicin resistance ...197548945
micrococcal urinary-tract infections in young a prospective study in young women, novobiocin-resistant subgroup-3 micrococci were the second commonest cause, after escherichia coli; of acute urinary infections. proteus mirabilis was the only other causative organism. symptoms, pyuria, or possible aetiological factors were the same in micrococcal and coliform infections. the infecting micrococcus "biotype" was only rarely found among the normal flora of the genitourinary tract of young women, though other micrococci and staphylococci were ...197551404
studies on lipopolysaccharides of proteus vulgaris serogroups. chemotypes of genus proteus lipopolysaccharides. 197555287
[serological activity of different antigenic preparations of proteus in precipitation tests]. 197555948
current questions on the tumour-associated antigens of chemically-induced tumours ii. in search of practical uses.with special reference to tumours which were chemically-induced in animals, we present a brief review on the applicability of the results obtained, as to immunoprophylaxis and to immunotherapy. a search is made onto what are the mostly representative animal models for human studies. from the present available data, experimental chemically-induced bladder and colonic tumours seem to be the "best" models for human counterparts.197657809
rational choice of penicillins and cephalosporins based on parallel in-vitro and in-vivo tests.because of the unavailability of strictly comparable data, seven representative penicillins and the five cephalosporins currently used in britain were evaluated in parallel, both in vitro and in vivo. penicillin sensitive and resistant strains of staphylococcus aureus and proteus mirabilis were the main test organisms. minimum bacteriocidal concentrations of cloxacillin, flucloxacillin, cephalothin, and cephazolin in serum were much higher than conventional minimum inhibitory concentrations in t ...197661353
a polyvalent human gamma-globulin immune to pseudomonas aeruginosa: passive protection of mice against lethal a means to development of guidelines for therapeutic application to human disease, preparations of human polyvalent gamma-globulin immune to pseudomonas aeruginosa (pg) were studied in acute infections in mice. pg was highly effective in controlling lethal infections induced in mice by the major immunotypes of p. aeruginosa; greater than or equal to 10 microgram of of gamma-globulin per mouse protected against challenge with less than or equal to 10(6) 50% lethal doses of p. aeruginosa. pg wa ...197770492
inhibition of phagocytosis in vitro by obligate anaerobes.a variety of species of obligate anaerobes have been shown to interfere with the phagocytosis and killing of proteus mirabilis and other aerobic bacteria in vitro. although all the obligate anaerobes examined showed this activity, the effect was greatest with strains of bacteroides melaninogenicus and b. fragilis. in contrast, none of the 36 aerobes tested acted in this way. these observations suggest that the presence of obligate anaerobes may be fundamental to the pathogenesis of some types of ...197773952
stomach as source of bacteria colonising respiratory tract during artificial ventilation.10 adults had intermittent positive-pressure ventilation for 3--34 days. all 10 had paralytic ileus. microbial overgrowth in the stomach was found in 9 patients (bacterial in 7 and fungal in 2); gram-negative bacteria predominated. the trachea invariably became colonised by bacteria, mainly gram-negative organisms. in 3 instances the gram-negative bacteria were found in the stomach before they appeared in the tracheal aspirate, and in 1 case the pathogen originated in the faeces.197881991
[effect of s-adenosyl-l-methionine on the cerebral and peripheral metabolism of l-dopa].the repartition of [3h]l-dopa administered i.p. does not change in rats treated with s-adenosyl-l-methionine (sam). the effect was studied on the central nervous system and at the periphery. the biosynthesis of metabolites [3h]dopamine and [3h]3-o-methyldopamine are also unchanged. on the other hand, in the kidney an accumulation of [3h]norepinephrine +[3h]normetanephrine was observed, while in the brainstem + midbrain the synthesis of these metabolites was decreased after sam injection. the rel ...197884550
study of the glycolipid composition of the milk fat-globule membrane and the mouse mammary tumour virus prepared from the milk of infected swiss mice [proceedings]. 197884579
[use of chromolymphotrast for color lymphography]. 197984768
dietary fibre and experimental colon cancer. 197985018
[determination of protein-sh groups with ddd reagent]. 197685309
[mast-cell degranulation in the intestine: its significance for the pathogenesis of galactosamine hepatitis. histological and histochemical studies on mast-cells using toluidine-blue and subsequent naphthol-asd-chloracetate reaction (author's transl)]. 197885385
comparative light- and electron-microscopical study of the normal adenohypophysis in the human.the pars distalis of 1 girl and of 3 sexually mature women was studied. the glands were dissected out and fixed within a period of 30 min following death; in the aldehyde fixative, each gland was systematically divided into ten pieces. before fixation in osmium tetroxide, each piece was again divided into several blocks. at least 60 blocks were obtained from each gland. semi-thin sections obtained from all the blocks were stained and studied under the light microscope. ultrathin sections were ob ...197985403
the spinal cord protein (scp): appraisal of physiological and immunological hypotheses of anti-encephalitogenic action. 197885602
in vitro synthesis of infectious transforming dna by the avian sarcoma virus reverse transcriptase.infectious dna molecules, capable of transforming chicken embryo fibroblasts, can be synthesized by the rous sarcoma virus-associated reverse transcriptase in vitro. the optimal enzymatic conditions employed for infectious dna synthesis also facilitate maximum synthesis of genome length dna. analysis of the dna product synthesized by detergent-disrupted rous sarcoma virus under these conditions indicates that dna complementary to viral rna (minus-strand dna) is genome length in size, whereas dna ...197985719
[method of preparing the tularemia antigen for an erythrocyte diagnosticum]. 197985742
urinary beta 2 microglobulin in upper and lower urinary-tract infections.beta 2-- microglobulin excretion in 24 h urine collections and beta2- microglobulin clearance were significantly increased in patients with upper-urinary-tract infections, but beta 2- microglobulin clearance and excretion were normal in patients with cystitis. beta 2-microglobulin estimation may be used to distinguish between upper and lower urinary-tract infections, except when pre-existing tubular damage is present. voided urine must have a ph above 6 . 0, since beta 2- microglobulin is rapidl ...197986044
the humoral and cell-mediated immune responses to somatic and metabolic antigens in rats infected with angiostrongylus cantonensis. 197886211
[intratypical antigenic differentiation of poliovirus strains circulating in mali by means of cross-adsorbed sera].the intratypic antigenic differentiation of poliovirus strains isolated from sick or healthy children in mali in 1975-1976 was performed. the method is based on the use of cross-adsorbed sera in the neutralization tests in which the virus is titrated by the plaque procedure or by the cytopathic effect. all the examined strains of serologic types i, ii, and iii were distinguished by the antigenic structure from the vaccine strains and were classified as "wild". quantitative differences in the ant ...197886237
serological investigations on ribitol-containing lipopolysaccharides from proteus mirabilis.lipopolysaccharides containing or noncontaining ribitol derived from several proteus mirabilis strains were studied using passive hemagglutination, hemagglutination-inhibition and semi-quantitative precipitin tests. the results indicate that ribitol plays a role in the serological specificity of the respective lipopolysaccharides.197886334
the role of lysine in the serological specificity of some proteus mirabilis lipopolysaccharides. 197886335
the isolated human cortex. a golgi analysis of krabbe's disease.the clinical course of a child with krabbe's leukodystrophy was characterized by clinical seizures, startle myoclonus, and paroxysmal activity recorded by eeg. at autopsy in the fourth year, myelinated subcortical axons were destroyed, virtually completely. despite isolation from major subcortical and interhemispheric connections, the cell and fiber pattern of the cortex appeared remarkably normal in routine histologic preparations. the normal range of pyramidal and stellate interneurons were al ...197986346
inhibition of neuronal firing by opiates: evidence against the involvement of cyclic nucleotides.1. extracellular recordings were made in vitro from single neurones of the myenteric plexus of the guinea-pig ileum. 2. neuronal firing was inhibited by morphine and normorphine (10 nm to 1 micrometer). cyclic adenosine 3',5'-monophosphate (cyclic amp) (100 micrometer to 1 mm) also inhibited the firing of the majority of the neurones. prostaglandin e2 usually caused a short-lasting excitation of myenteric neurones and the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine was usually withou ...197986371
the phenomenon of differentiation in murine erythroleukemic cells. 197886529
cytofluorometric and cytochemical comparisons of normal and abnormal human cells from the female genital tract.acridine orange staining of exfoliated cells from epithelial tissues facilitates discrimination between normal and abnormal cells: abnormal cells develop highly elevated nuclear fluorescence. comparisons of acridine orange (ao) staining with propidium iodide (pi) or feulgen staining have shown that: (a) pi staining also provides highly elevated nuclear fluorescence from abnormal cells; (b) the distributions of nuclear fluorescence following ao or pi staining were usually not significantly differ ...197986579
reinforcing stimulation of the postero-lateral hypothalamus and mating behaviour in the rat.male rats implanted with chronic electrodes into the postero-lateral hypothalamic site were tested for self-stimulation behaviour. rats exhibiting steady self-stimulation behaviour were observed during mating tests with an oestrus female. during these tests the hypothalamus of male rats was stimulated. results show that no stimulus-bound sexual behaviour was observed. nevertheless, correlations were found between the rewarding value of the cerebral stimulation and the specific sexual components ...197887181
similarity of casein- and endotoxin-induced, myeloma- associated and aged sjl/j amyloid in various strains of mice.amyloidosis was induced in a number of strains of mice by repeated injections of casein and endotoxin. spontaneous amyloid was obtained from balb/c mice bearing a myeloma tumor (igg2a producing mopc 173 tumor) and from aged sjl/j mice. both the induced and spontaneous forms were similar in their size, immunological reactivity, peptide maps and in the susceptibility of histological sections to oxidizing agents with or without trypsin digestion. since case-induced murine amyloid resembles the noni ...197987374
moloney virus-induced cell surface antigens and histocompatibility antigens are located on distinct molecules. 197987439
human corneal endothelium in organ culture. the influence of temperature and medium of incubation.forty-four human corneas from patients between 21 and 86 years were incubated in eagle's minimum essential medium with earle's salts 10--46 h post mortem. the influence of incubation temperature and composition of the medium on endothelial survival was evaluated. whole corneas were stained by alizarine red. recent cell loss was indicated by morphological alterations in the endothelial pattern. after 20--28 h of incubation minimum cell loss was found at 31 degrees c when 8% dextrane-250 and 20% s ...197988158
denaturization of allergen p: effect on allergenicity, antigenicity and immunogenicity.when allergen p was denatured by 8m urea, the modified molecule still reacted with ige specific for the native allergen but not with hemagglutinating antibodies. heating at 100 degrees c abolished the reaction in both cases. the results suggested differences between allergenic and antigenic capacities which may be based on structural differences of the antigenic determinants.197988195
the role of p23,30-bearing human macrophages in antigen-induced t lymphocyte responses. 197988270
fuorescent retrograde neuronal labeling in rat by means of substances binding specifically to adenine-thymine rich dna.six fluorescent substances, binding specifically to adenine-thymine rich dna, were injected in rat caudate-putamen. this resulted in retrograde axonal transport and fluorescent retrograde labeling of neurons in center-medium parafascicular nucleus, substantia nigra and dorsal raphe. two of these substances, i.e. "true blue' and 'granular blue', give a very striking blue fluorescent retrograde neuronal labeling. mid-thoracic spinal injections of these two substances in rat also resulted in a pron ...197988697
dansylcadaverine specific staining for transamidating enzymes. 197988908
a new assay of estrogen receptor by thin-layer gel filtration. 197988911
[post-extrasystolic and post-pacing potentiation. i. experimental study of the isolated heart of the rabbit]. 197888947
reaction of cells from early chick embryos with antiserum against mouse sperm cells. 197989009
binding specificity of rat alpha-fetoprotein for a series of estrogen derivatives: studies using equilibrium and nonequilibrium binding techniques.the binding specificity of rat alpha-fetoprotein (afp) for a series of estrogen derivatives has been investigated and compared to that of the uterine cytosol estrogen receptor. in addition, several equilibrium and nonequilibrium binding techniques have been examined for their appropriateness in studying afp-estrogen interactions under different experimental conditions. the ratio of association constants (rac) of various estrogen derivatives (compared to 17 beta-estradiol; rac = 100%) were determ ...197989027
trasylol prevents trypsin-induced shock in dogs.the effect of simultaneous intravenous administration in the dog of bovine trypsin and trasylol followed by continued infusion of trasylol was studied. special attention was paid to the interchange between the dominating plasma protease inhibitors alpha1-antitrypsin and a-macroglobulins and to the disappearance of trasylol and its trypsin complexes from the circulation. the following results were obtained: 1) trypsin was preferentially bound by the alpha-macroglobulins, though trasylol is a stro ...197989067
differential inducibility of epstein-barr virus in cloned, non-producer raji cells.cells of the human lymphoblastoid non-producer line raji were cloned in soft agar. individual colonies were isolated and analyzed for their inducibility of the epstein-barr virus-associated early antigen (ea). the induction of ea by the tumor promoter tpa varied among the different cell clones. clones with very high and very low inducibility of the resident epstein-barr virus genome were further analyzed. constant differences in the inducibility of ea were observed after activation by tumor prom ...197989100
preparative isolation of the cell receptor for immunoglobulin e. 197989114
control of v kappa expression in the mouse. i. unexpected expression of the v kappa allele, igk-pcb, in a somatic cell hybrid of akr (igk-pca) origin. 197989158
inheritance of tolerance susceptibility to human gamma-globulin in congenic mice.the genetic control of susceptibility to tolerance induction with human gamma-globulin (hgg) was studied by using h-2 congenic mice. strains tested that were congenic with c57bl/10sn were completely tolerized by 1.0 mg deaggregated hgg. in contrast a/sn mice showed full tolerance whereas a.sw mice were only intermediately tolerant. it was further shown that (b10 x sjl)f1 mice could be rendered tolerant but (b10.s x sjl)f1 mice could not. these data indicate a role for h-2 linked genes in control ...197989162
antigenic changes associated with liver carcinogenesis.carcinogen-induced experimental hepatomas are often characterized by new individually distinct antigens capable of inducing tumor immunity in syngeneic hosts. these antigens arise as a consequence of cell-carcinogen interaction and may result from modification or replacement of normal cell-surface components. their role in immunosurveillance is not established, but they offer a target for tumor immunotherapy. reexpressed fetal antigens have also been detected, either as secretory products (alpha ...197989200
[preparation of antisera by combined administration of antigens]. 197989268
the influence of decalcifying fluids on the demonstration of mycobacterium tuberculosis in paraffin sections. 197989611
monkey antiserum to mlc-primed human t lymphocytes. 197989737
histological diagnosis of myocardial injury. comparison of hematoxylin-basic fuchsin-picric acid (hbfp)-stained sections obtained during autopsy with isolated viable rat cardiac myocytes exposed to anoxia.the light microscopic appearances in hematoxylin-basic fuchsin-picric acid (hbfp)-stained histological sections from cardiac and skeletal muscle tissue were put in relation to the reactions of isolated viable rat cardiac myocytes exposed to anoxia in suspension and their morphology in paraffin-embedded sections. special attention was paid to prenecrotic phases of myocytic injury which were followed, in viable rat cardiac myocytes, by light microscopy, and confirmed with biochemical assays indica ...197989781
histamine-releasing effect of a corticotrophin derivative. ii. mechanism of action of histamine release by c 44 680-ba, compared with that of cpd. 48/80, dextran and triton.the mechanism of the histamine-liberating action of the synthetic polypeptide c 44 680-ba, an alkyl-prolyl derivative of beta 1-19 corticotrophin, was investigated and compared with those of compound 48/80, dextran, melittin and triton x-100. it was found that the release of histamine from rat peritoneal cells induced by the polypeptide is dependent on temperature, ph, calcium ions and energy-providing processes. in regard to these criteria, the mode of action of this histamine liberator resembl ...197989804
steroid secretion by in vitro perfused testes: inhibitors of testosterone biosynthesis. 197989816
effect of encephlitogenic protein. ppd and tetanus toxoid on leukocyte migration in agarose. a study of "cross-reactivity".the reactivity to three antigens: bovine encephalitogenic protein (ep), ppd, and tetanus toxoid, was studied with blood leukocytes from healthy humans using clausen's (5) leukocyte migration in agarose technique. there is an obvious correlation between the reaction of ep (all concentrations studied) and to low concentrations of ppd; and between the reactivity to low concentrations of ep and low concentrations of tetanus toxoid. after vaccination with tetanus vaccine, a marked increase in reactiv ...197989821
antibodies in histoplasmosis detected by use of yeast and mycelial antigens in immunodiffusion and electroimmunodiffusion. 197989825
inhibition of histamine release from rat mast cells by cytochalasin a and other sulfhydryl reagents. 197989848
alpha-fetoprotein as a carrier protein in plasma and its bilirubin-binding ability.the bilirubin-binding ability of human alpha-fetoproteins, which were purified from fetal cord serum and from ascites fluid of a hepatoma-bearing patient, was examined by the difference spectrum and the jacobsen peroxidase methods. the difference spectrum observed as a result of the specific binding of bilirubin to alpha-fetoprotein had a maximum at 482 nm, and this pattern was quite similar to that observed for serum albumin. the result obtained by the difference spectrum method showed that 1 m ...197989900
rat alpha-macrofetoprotein (acute-phase alpha 2-macroglobulin) during hepatocarcinogenesis.rat alpha-macrofetoprotein (amf) and alpha-fetoprotein (afp) are secreted by the fetal liver and become elevated in serum during hepatocarcinogenesis and in animals bearing hepatocellular carcinomas. it has been suggested that these fetal plasma proteins may be influenced by related control mechanisms. the experiments presented herein examined the early responses of these plasma proteins during hepatocarcinogenesis using the hepatocarcinogens acetylaminofluorene and diethylnitrosamine. under the ...197989903
coupling between neuronal activity and focal blood flow in experimental seizures.local blood flow, ecog and single cortical neurone activity were recorded simultaneously from single microelectrodes in 17 cats. seizures were induced by repeated intravenous injections of pentylenetetrazol (ptz, 10-20 mg/kg) or by local application of 1 m na-penicillin. seven to 20 sec after appearance of burst activity in cortical neurones and ecog, focal flow increased up to 300% of control. the extent of this flow increase was significantly correlated with the change in firing rate of the ne ...197989943
pattern of condensation of mouse and chinese hamster chromosomes in g2 and mitosis of 33258-hoechst-treated cells. 197989960
revertants of adenovirus type-12-transformed hamster cells have lost part of the viral genomes. 197990022
enzymatic tracers in the study of vascular permeability.elucidation of the ultrastructural basis of vascular permeability was aided by the development of cytochemical techniques for visualizing the distribution, within the vessel wall, of intravenously injected peroxidatic enzymes of varying molecular size. tracer enzymes available range from 10 a (hemeoctapeptide) to 52 a (catalase) effective molecular radius. the use of enzymatic probe molecules assumes a thorough characterization of: (a) the molecular charge (isoelectric point of the native enzyme ...197990073
modulation of the host response in human schistosomiasis. i. adherent suppressor cells that inhibit lymphocyte proliferative responses to parasite antigens. 197990093
m467: a murine iga myeloma protein that binds a bacterial protein. i. recognition of common antigenic determinants on salmonella flagellins.we have studied the binding of m467, an iga murine myeloma protein, to flagellin from seven species of salmonella. it was found that m467 was reacting with antigenic determinants that were common to all the flagellins studied. these determinants were not related to serotypic antigens. electronmicrographs of unreduced m467 showed a variety of polymeric species bound to flagella in a manner that could produce immobilization as well as agglutination and precipitation through cross-linking of antige ...197990100
biosynthesis of an unglycosylated envelope glycoprotein of rous sarcoma virus in the presence of tunicamycin.cells stably infected with rous sarcoma virus were treated with tunicamycin to prevent the glycosylation of the precursor (pr92gp) to the two viral envelope glycoproteins gp85 and gp35. pretreatment of the cells for 4 h with the antibiotic resulted in a 90% reduction in [3h]mannose incorporation into total cellular glycoproteins, intracellular viral glycoproteins, and released virus particles. protein synthesis and virus particle formation were not significantly affected by the treatment. a new ...197990167
[pharmacological properties and antitumor activity of a preparation diiodo-benzo-tepa in experiments].experimental data on the pharmacokinetics, toxicity, antitumor activity of the antitumor drug diiodo-benzo-tepa are reported.197990429
cells of origin of the spinocerebellar tract in the rat, studied with the method of retrograde transport of horseradish peroxidase.following injections of horseradish peroxidase into the cerebellum, the distribution of labeled neurons was studied in the whole length of the spinal cord of the rat. to find the ascending side of the axons, injections were made following hemisections at c1 or between c1 and c2. labeled spinocerebellar tract neurons were classified into two groups according to the axonal course in the spinal cord; one is composed of neurons with uncrossed ascending axons and the other, neurons with crossed ascen ...197990539
in vitro studies of the replication of moloney murine leukemia virus. 197990575
cell differentiation in human gastric gland as revealed by nuclear binding of tritiated actinomycin.the nuclear binding of h3 actinomycin, which is closely linked to the differentiation phenomenon, was studied in human normal gastric mucosa. actinomycin binding decreases in cells which differentiate and becomes very low in fully differentiated cells. in the gastric pits, there is a decreasing gradient of labelling from the deeper stem cells to the well-differentiated superficial cells. this indicates that migration and renewal of the surface epithelium occurs following a 'pipe-line' system. al ...197990638
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