approach to a practical method for screening and identifying microorganism genera from urine (author's transl).in this study the author reported upon a practical new system for screening and identifying the microbial agents causing urinary tract infections. this system is composed of a combination of 3 screening procedures (ph-value + nitrite-test + catalase-test) and 8 selective culture media for the purpose of genus identification within 24 hours (uripret-g). a total of 130 cultures was investigated. the employed microorganisms were mainly recovered from urine samples. they included the following speci ...197611179
effect of oligomycin on coupling in isolated mitochondria from oligomycin-resistant mutants of saccharomyces cerevisiae carrying an oligomycin-resistant atp phosphohydrolase. 197720055
1-n hapa gentamicin b, a new aminoglycoside active against gentamicin resistant isolates--activity compared to other aminoglycosides.1-n hapa gentamicin b is a new aminoglycoside active against most enterobacteriaceae, pseudomonas aeruginosa and staphylococcus aureus. among 504 clinical isolates at a concentration of 12.5 microgram/ml all staph. aureus, escherichia coli, klebsiella, enterobacter, proteus rettgeri, providencia and 78% of pseudomonas, 86% of proteus morganii were inhibited. like other aminoglycosides, the activity was greatest at an alkaline ph and reduced by high cations concentrations. 1-n hapa gentamicin b w ...197827494
antigenic relationship between morganella morganii and yersinia enterocolitica.two new o antigens have been described for the morganella morganii antigenic schema. o antigens of the strains representing the new serotypes (o43 :h2 and o44 : h19, 37) are related to yersinia enterocolitica o9 and o17, respectively.197659520
antigenic specificity of cell-mediated antitumor reaction in the msv system. 197560997
purification and properties of beta-lactamase from proteus morganii.the cephalosporin beta-lactamase was purified from a strain of proteus morganii that showed resistance to beta-lactam antibiotics and produced the enzyme constitutively. the purified enzyme preparation gave a single protein band on polyacrylamide gel electrophoresis and consisted of a single polypeptide of molecular weight 38,000 to 40,000 from gel filtration of sephadex g-100 and sodium dodecyl sulfate-acrylamide gel electrophoresis, its isoelectric point being ph 7.2 no cysteine residue was fo ...197770426
[use of chromolymphotrast for color lymphography]. 197984768
the phenomenon of differentiation in murine erythroleukemic cells. 197886529
ultrastructure of bacteroides species: bacteroides asaccharolyticus, bacteroides fragilis, bacteroides melaninogenicus subspecies melaninogenicus, and b. melaninogenicus subspecies intermedius.representative strains of two subspecies of bacteroides melaninogenicus (subspecies melaninogenicus and subspecies intermedius) and bacteroides asaccharolyticus as well as b. asaccharolyticus strain 536b isolated from a human perirectal abscess and bacteroides fragilis atcc 25285 were examined by glutaraldehyde-osmium fixation, ruthenium red fixation and staining, and thorium hydroxyde staining as well as by the physical preparative techniques of critical point drying--transmission electron micr ...197986590
similarity of casein- and endotoxin-induced, myeloma- associated and aged sjl/j amyloid in various strains of mice.amyloidosis was induced in a number of strains of mice by repeated injections of casein and endotoxin. spontaneous amyloid was obtained from balb/c mice bearing a myeloma tumor (igg2a producing mopc 173 tumor) and from aged sjl/j mice. both the induced and spontaneous forms were similar in their size, immunological reactivity, peptide maps and in the susceptibility of histological sections to oxidizing agents with or without trypsin digestion. since case-induced murine amyloid resembles the noni ...197987374
demonstration of an alpha2-macroglobulin receptor in human fibroblasts, absent in tumor-derived cell lines. 197987392
moloney virus-induced cell surface antigens and histocompatibility antigens are located on distinct molecules. 197987439
human corneal endothelium in organ culture. the influence of temperature and medium of incubation.forty-four human corneas from patients between 21 and 86 years were incubated in eagle's minimum essential medium with earle's salts 10--46 h post mortem. the influence of incubation temperature and composition of the medium on endothelial survival was evaluated. whole corneas were stained by alizarine red. recent cell loss was indicated by morphological alterations in the endothelial pattern. after 20--28 h of incubation minimum cell loss was found at 31 degrees c when 8% dextrane-250 and 20% s ...197988158
denaturization of allergen p: effect on allergenicity, antigenicity and immunogenicity.when allergen p was denatured by 8m urea, the modified molecule still reacted with ige specific for the native allergen but not with hemagglutinating antibodies. heating at 100 degrees c abolished the reaction in both cases. the results suggested differences between allergenic and antigenic capacities which may be based on structural differences of the antigenic determinants.197988195
the role of p23,30-bearing human macrophages in antigen-induced t lymphocyte responses. 197988270
fuorescent retrograde neuronal labeling in rat by means of substances binding specifically to adenine-thymine rich dna.six fluorescent substances, binding specifically to adenine-thymine rich dna, were injected in rat caudate-putamen. this resulted in retrograde axonal transport and fluorescent retrograde labeling of neurons in center-medium parafascicular nucleus, substantia nigra and dorsal raphe. two of these substances, i.e. "true blue' and 'granular blue', give a very striking blue fluorescent retrograde neuronal labeling. mid-thoracic spinal injections of these two substances in rat also resulted in a pron ...197988697
[post-extrasystolic and post-pacing potentiation. i. experimental study of the isolated heart of the rabbit]. 197888947
reaction of cells from early chick embryos with antiserum against mouse sperm cells. 197989009
immunological unresponsiveness in mice. ii. cellular basis of immunological unresponsiveness induced in foetal and neonatal mice by transfer of human gamma-globulin by the maternal route.the cellular basis of the mechanism of immunological tolerance to human gamma-globulin (h gamma g) induced in foetal and neonatal mice by materno-foetal or materno-neonatal transfer after a single injection of tolerogen (deaggregated h gamma g) into the mothers was investigated using a cell transfer system and assays of passive haemagglutinating antibodies and plaque-forming cells to h gamma g. the results demonstrated that b cells are mainly involved in the tolerance induced on the fourteenth d ...197989080
differential inducibility of epstein-barr virus in cloned, non-producer raji cells.cells of the human lymphoblastoid non-producer line raji were cloned in soft agar. individual colonies were isolated and analyzed for their inducibility of the epstein-barr virus-associated early antigen (ea). the induction of ea by the tumor promoter tpa varied among the different cell clones. clones with very high and very low inducibility of the resident epstein-barr virus genome were further analyzed. constant differences in the inducibility of ea were observed after activation by tumor prom ...197989100
preparative isolation of the cell receptor for immunoglobulin e. 197989114
control of v kappa expression in the mouse. i. unexpected expression of the v kappa allele, igk-pcb, in a somatic cell hybrid of akr (igk-pca) origin. 197989158
antigenic changes associated with liver carcinogenesis.carcinogen-induced experimental hepatomas are often characterized by new individually distinct antigens capable of inducing tumor immunity in syngeneic hosts. these antigens arise as a consequence of cell-carcinogen interaction and may result from modification or replacement of normal cell-surface components. their role in immunosurveillance is not established, but they offer a target for tumor immunotherapy. reexpressed fetal antigens have also been detected, either as secretory products (alpha ...197989200
antigenic analysis of human heart tissue--characterization of cardiac antigens.the human heart antigens demonstrated in citric acid extracts by immunodiffusion were submitted to further analysis. heart antigens were precipitated at 30% to 70% saturated ammonium sulphate. these fractions contained antigens reactive with rabbit antisera to human heart. the third fraction out of four proteins which were separated by sephadex g-150 gel filtration, was reactive against rabbit antisera to human heart. the intensely stained proteins of both heart antigens were located at the nine ...197989208
[change in the thresholds of development of ventricular arrhythmias after stimulation of negative emotiogenic hypothalamic centers].the dynamics of changes in the thresholds of the origin of ventricular extrasystole, paroxysmal ventricular tachysystole and ventricular fibrillation in long-term stimulation of the ventromedial hypothalamic nucleus were studied in experiments on rabbits. it was found that myocardial resistance to the development of arrhythmias depended on the manifestation of the sympathetico-parasympathic interaction on the heart. in predominance of parasympathetic effects on the heart during hypothalamic stim ...197989222
cimetidine: interaction with oral anticoagulants in 6 patients anticoagulated with warfarin, nicoumalone, or phenindione the addition of cimetidine prolonged the prothrombin-time (pt) by a mean of 12.6 s (range 5--23 s). in 7 volunteers taking daily subtherapeutic doses of warfarin the addition of cimetidine increased the pt from 19.4 to 22.9 s and the plasma-warfarin concentration from 0.96 to 1.76 microgram/ml. cimetidine reduced the single-dose clearance of warfarin and antipyrine. the basis of the interaction between cimetidine and oral an ...197989387
monkey antiserum to mlc-primed human t lymphocytes. 197989737
observations concerning the staining properties of the macroconidia of certain dermatophyton species.staining of the macroconidia of several dermatophyton species by lactophenol-cotton blue was investigated. young macroconidia stain variably; their cytoplasm may appear homogeneous or inhomogeneous. the possible explanation seems to be variation in the composition of the cytoplasm. in some macroconidia dark-staining filament was seen along the longitudinal axis. mature macroconidia showed basal or basal+apical homogeneous deep staining. the intensive apical staining suggests that the apical stru ...197889788
effect of encephlitogenic protein. ppd and tetanus toxoid on leukocyte migration in agarose. a study of "cross-reactivity".the reactivity to three antigens: bovine encephalitogenic protein (ep), ppd, and tetanus toxoid, was studied with blood leukocytes from healthy humans using clausen's (5) leukocyte migration in agarose technique. there is an obvious correlation between the reaction of ep (all concentrations studied) and to low concentrations of ppd; and between the reactivity to low concentrations of ep and low concentrations of tetanus toxoid. after vaccination with tetanus vaccine, a marked increase in reactiv ...197989821
antibodies in histoplasmosis detected by use of yeast and mycelial antigens in immunodiffusion and electroimmunodiffusion. 197989825
modulation of cellular interactions between c3h/10t1/2 cells and their transformed counterparts by phosphodiesterase has been demonstrated previously that nontransformed c3h/10t1/2cl8 mouse embryo fibroblasts (10t1/2) can induce a state of reversible growth inhibition in cocultured malignantly transformed mouse fibroblasts and that this inhibition is modulated by serum concentration. the present study suggests that cyclic nucleotides may be implicated in this intercellular communication. the phosphodiesterase inhibitors theophylline, caffeine, and 3-isobutyl-1-methylxanthine (ibx) at concentrations of 10(-3 ...197989899
alpha-fetoprotein as a carrier protein in plasma and its bilirubin-binding ability.the bilirubin-binding ability of human alpha-fetoproteins, which were purified from fetal cord serum and from ascites fluid of a hepatoma-bearing patient, was examined by the difference spectrum and the jacobsen peroxidase methods. the difference spectrum observed as a result of the specific binding of bilirubin to alpha-fetoprotein had a maximum at 482 nm, and this pattern was quite similar to that observed for serum albumin. the result obtained by the difference spectrum method showed that 1 m ...197989900
coupling between neuronal activity and focal blood flow in experimental seizures.local blood flow, ecog and single cortical neurone activity were recorded simultaneously from single microelectrodes in 17 cats. seizures were induced by repeated intravenous injections of pentylenetetrazol (ptz, 10-20 mg/kg) or by local application of 1 m na-penicillin. seven to 20 sec after appearance of burst activity in cortical neurones and ecog, focal flow increased up to 300% of control. the extent of this flow increase was significantly correlated with the change in firing rate of the ne ...197989943
revertants of adenovirus type-12-transformed hamster cells have lost part of the viral genomes. 197990022
the association of carbohydrate with tubulin and in vitro assembled microtubules from bovine brain.phosphocellulose-purified tubulin (pc tubulin) was analyzed for neutral and amino sugar content, which was found to be 8.3 +/- 0.11 and 0.8 +/- 0.02 mol/mol dimer, respectively. a histochemical-electron-microscopic investigation was undertaken to attempt to localize carbohydrate associated with polymerized microtubules (mt). outer diameters of mt assembled in vitro from bovine brain mt protein (tubulin and microtubule associated proteins) were found to increase upon treatment with ruthenium red, ...197990041
m467: a murine iga myeloma protein that binds a bacterial protein. i. recognition of common antigenic determinants on salmonella flagellins.we have studied the binding of m467, an iga murine myeloma protein, to flagellin from seven species of salmonella. it was found that m467 was reacting with antigenic determinants that were common to all the flagellins studied. these determinants were not related to serotypic antigens. electronmicrographs of unreduced m467 showed a variety of polymeric species bound to flagella in a manner that could produce immobilization as well as agglutination and precipitation through cross-linking of antige ...197990100
characterization of in vitro proliferative responses of human lymphocytes to leishmanial antigens.leishmaniasis is an intracellular protozoal infection for which host defense is believed to depend on cellular immune mechanisms. the in vitro proliferative responses of lymphocytes from patients with leishmaniasis and from control subjects to leishmanial antigens were examined. only lymphocytes from patients responded to 1 microgram of leishmanial antigen/ml, whereas both patient and control lymphocytes responded to 10 micrograms/ml. the nonspecific responses were most likely not due to a mitog ...197990105
differential growth inhibition in two human carcinoma cell lines by cyclic adenosine 5'-monophosphate analogs.the inhibition of cell replication in two human carcinoma cell lines by various cyclic amp analogs was explored. in all instances, the addition of the cyclic nucleotide phosphodiesterase inhibitor 1-methyl-3-isoburylxanthine resulted in synergistic growth inhibition by the analogs. a correlation was found between an analog's ability to inhibit growth and its ability to activate protein kinase. a differential effect of the breakdown product 8-bromo-amp (8-bramp) on cell replication in the two cel ...197990151
in vitro studies of the replication of moloney murine leukemia virus. 197990575
antigenic determinants in the post-mitochondrial lung fraction. 197990649
purification of a new high activity form of glucose-6-phosphate dehydrogenase from rat liver and the effect of enzyme inactivation on its immunochemical reactivity.a new form of cytoplasmic glucose-6-phosphate dehydrogenase (e.c. was purified from rat liver by protamine sulfate precipitation, ammonium sulfate fractionation, ion exchange chromatography with diethylaminoethyl cellulose, and affinity chromatography with cibacron blue agarose and nadp agarose. this form of the enzyme has a specific activity of over 600 units/mg of protein and gives essentially a single band by polyacrylamide gel electrophoresis. the form of the enzyme isolated by this ...197990677
the role of eosinophil receptors in the non-genomic response to oestrogens in the uterus. 197990751
connectivity of the auditory forebrain nuclei in the guinea fowl (numida meleagris).injection of tritiated leucine and proline into the nucleus ovoidalis of the guinea fowl (numida meleagris) produces terminal labeling in the palaeostriatum and in three adjacent zones (field l1-l3) of the auditory neostriatum (an). l2, situated between l1 and l3, receives the main input and corresponds to the former field l of rose. these neuroanatomically defined zones of the auditory neostriatum are also characterized by differing properties of their neurons. injection of radioactive material ...197991442
morphochemic studies on histamine and monoaminooxidase in the liver and studies on histaminolytic activity of guinea pig plasma in the course of sensitization with heterogenic protein. 197991564
["non-specific cross reacting antigen (nca)", a tracer of human granulocytes and monocytes].the non specific cross reacting antigen, or nca, is a normal tissue antigen that cross reacts with cea. it bears a specific antigenic determinant that is absent from cea. immunocytological studies first pointed out that nca, but not cea, is present in alveolar macrophages and polymorphonuclears. further work demonstrated that nca is a marker of granulocytic series: it appears at the stage of promyelocyte and likely is linked to the azurophilic granules. the same antigen is also present in periph ...197992357
nonspecific light loss and intrinsic dna variation problems associated with feulgen dna cytophotometry.nonspecific light loss by the cell-wall-plus-cytoplasm (cwc) can cause a 50% increase in feulgen absorption units in peanut root-tip nuclei as determined by scanning at 450 nm, whereas this phenomenon is not evident with chicken erythrocytes. a two wavelength scanning method of subtracting nonspecific 450 nm absorption from 550 nm feulgen absorption values eliminated the nonspecific light loss in cwc, however, the two wavelength scanning method is time consuming and somewhat impractical with a r ...197992496
[histamine liberation in the fish meat infected with proteus morgani]. 197992805
location of bladder preganglionic neurons within the sacral parasympathetic nucleus of the cat.sacral parasympathetic preganglionic neurons innervating the urinary bladder were labelled by applying horseradish peroxidase (hrp) to branches of the pelvic nerve at the neck of the bladder. bladder preganglionic cells were located primarily in the intermediolateral grey matter of sacral cord segments 1, 2 and 3 and extended from the ventral end of the central canal to the inferior margin of the dorsal horn. dendrites extended into the dorsolateral funiculus, toward the lateral edge of the dors ...197993725
antifetal and tumor-specific reactivity in human osteosarcoma. 197994187
immunodiagnosis of systemic candidiasis: mannan antigenemia detected by radioimmunoassay in experimental and human infections.a radioimmunoassay (ria) that detects candida mannan was developed so that immunodiagnosis of systemic candidiasis could be improved. the ria was evaluated in an animal model of disseminated disease and in a panel of patient sera. mannan antigenemia was detected with the ria in 52% of 29 rabbits with systemic candidasis, but not in 60 normal rabbits or 31 rabbits with systemic aspergillosis. in an evaluation of human sera, mannan antigenemia was detected in five of 11 patients with systemic cand ...197994341
comparison of the antibodies elicited by the individual structural polypeptides of foot-and mouth disease and polio viruses.antibody produced against preparations of vp1, one of the four structural polypeptides of foot-and-mouth disease virus, neutralized the virus and reacted with both full and empty particles in radioimmunoassays (ria). antiserum against vp2 reacted with artificial empty particles of the virus but not with full particles. in contrast, none of the individual polypeptides of poliovirus produced antisera which neutralized the virus nor reacted with it in ria. however, antisera produced with vp1 and vp ...197994352
thyroid hormone regulation of alpha-adrenergic receptors: studies in rat myocardium.the effects of alterations in thyroid state on cardiac alpha-adrenergic receptors were investigated by the binding of [3h]dihydroergocryptine (dhe), a potent alpha-adrenergic antagonist. in seven experiments, cardiac membranes from euthyroid rats bound 47 +/- 9 fmoles dhe/mg protein (mean +/- se) at saturation and demonstrated a dissociation constant (kd) of 2.5 +/- 0.4 nm. hyperthyroidism, produced by parenteral injection of triiodothyronine, significantly reduced the binding of dhe at all conc ...197994388
[ca++-dependent amylase secretion by the pig submandibular gland slices in vitro]. 197995119
[immunological problems in oncology (author's transl)].the authors, after a view of the problems of immunology, regarding the most recent discoveries, deal with the conclusions about the immunological reactions in research and clinical oncology. many specific antibodies, formed as a result of the tumor, have been discovered in the field of research. it is impossible to say the same about human tumors and the probable hypothesis are numerous. the results until now obtained, even if they don't give a definite response, show that the immune defense of ...197995475
group f, a new vibrio? 197795779
lower levels of thyrotropin-releasing hormone-degrading activity in human cord and in maternal sera than in the serum of euthyroid, nonpregnant adults.thyrotropin-releasing hormone (trh)-degrading activity was investigated in human cord, maternal, and euthyroid adult sera by measuring (a) the rate of disappearance of trh and (b) the rate of formation of degradation products. the rate of trh degradation in cord and maternal sera was 25-33% of that in euthyroid adult serum. concomitantly, in cord and maternal sera, the rate of formation of proline, a major trh degradation product in serum, was one-quarter to one-third that in euthyroid adult ser ...197896140
[disinfection of gutta-percha cones]. 197796557
beta-lactamases: determination of their isoelectric points.important discrepancies in isoelectric points (pi) of beta-lactamases were observed depending on the experimental procedure used for their determination: isoelectric focusing (ief) in sucrose density gradients or analytical ief in thin-layer polyacrylamide gels (pa). the variations, negligable in the case of tem-like beta-lactamases, appeared to be important in the case of cephalosporinases and are related to an artifact which appears in pa-ief. this has been clearly shown with beta-lactamase pr ...197896731
[dna polymerases in human platelets].human platelets have two dna polymerases. in the mitochondrial-free cytoplasm we have found a dna polymerase gamma and another dna polymerase very closely related to the polymerase alpha from animal cells. in the mitochondria only the gamma activity is present.197896974
[gastric candidiasis. report of a case].one patient was seen with candida albicans infection in a gastric ulcer. gastric biopsy was of value in the diagnosis. the patient responded well to nistatin therapy.197797165
chymotrypsinlike activity of some intestinal bacteria.some tests of exocrine pancreatic function are based on the estimation of intestinal chymotryptic activity by using specific substrates such as n-benzoyl-l-tyrosyl-paba (bt-p). in this study the action of a number of bacteria isolated from human or monkey intestines on bt-p was investigated. the monkeys studied had an intestinal flora closely resembling that of man. except for bacteroides, proteus vulgaris no. 73, proteus morgani no. 202, and pseudomonas aeruginosa no. 76, significant splitting ...197898039
identification of cytoplasmic tubulin in dictyostelium discoideum. 197898348
effect of hypotension induced by sodium nitrocyanoferrate (iii) on the release of arginine-vasopressin in the unanaesthetized monkey. 1978101639
mutations altering the cellular localization of the phage lambda receptor, an escherichia coli outer membrane protein.two mutant strains of escherichia coli have been isolated in which the cellular location of an outer membrane protein, the phage lambda receptor (the lamb gene product), is altered. these mutations were initially selected in a strain containing a lamb-lacz fusion. in the parent strain the protein coded for by the hybrid gene is located, at least in part, in the outer membrane. in the mutants it is located in the cytoplasm. the mutations responsible for the alteration of cellular location lie ver ...1978104291
the fate of transplanted pancreatic islets in the rat.syngeneic pancreatic islets transplanted into the liver or the spleen reverse streptozotocin-induced diabetes in the rat, but allogeneic islets function only briefly and are rejected. shortly after transplantation, thrombi often form around transplanted tissue, particularly around nonislet tissue that contaminates islet preparations. these thrombi are a source of transient liver injury in recipients of intrahepatic grafts. a few days after transplantation, syngeneic islets injected into the port ...1979104625
activity of bepridil and other anti-anginals on cardiovascular modifications engendered by conditioned anxiety in the dog.the action of bepridil (5 mg/kg), perhexilene (5 mg/kg) propranolol (0.5 mg/kg), dipyridamole (0.5 mg/kg), amiodarone (10 mg/kg), pentaerythritol tetranitrate (40 microgram/kg) and nitroglycerine (40 microgram/kg) on cardiovascular manifestations of auditory-inducted emotional stress has been investigated in the conscious dog. bepridil, perhexilene and propranolol considerably reduce tachycardia provoked by the anxiogenic stimulus, with nitroglycerin exerting an attenuated effect and the others ...1978104675
bronchial mucosal permeability.the tracheobronchial epithelium has well-developed tight junctions which on a morphologic basis should be markedly resistant to penetration by protein molecules. despite this, antigen inhalation in monkeys allergic to ascaris suum results in the rapid onset of pulmonary physiologic changes. recent studies in man and animals have shown that a substantial number of mast cells exist in the bronchial lumen and epithelium. we suggest that antigen-antibody interaction initially occurs on these superfi ...1979104888
transcription and translation in a pleiotropic streptomycin-resistant mutant of escherichia coli.the role of the ribosomal protein s12 (streptomycin protein) in ribosome function and in other metabolic processes in the cell has been investigated. a spontaneous streptomycin-resistant strain of escherichia coli (sm3) carrying a mutation in the rpsl gene is deficient in its ability to induce the synthesis of the enzyme bets-galactosidase. it was demonstrated that the reduced rate of enzyme synthesis results from deficiencies in both the transcription of the lactose operon and translation of th ...1979104958
isolation of an iron-binding compound from pseudomonas iron-binding compound was isolated from ethyl acetate extracts of culture supernatant fluids of pseudomonas aeruginosa and was purified by successive paper and thin-layer chromatographic procedures. the purified compound was characterized by uv, visible, infrared, and fluorescence spectroscopy. the compound possesses phenolic characteristics, with little or no similarity to dihydroxybenzoates and no indication of a hydroxamate group. p. aeruginosa synthesized the compound during active growth ...1979104968
[isolation of treponemas from the colon of pigs with clinical dysentery].optimal culture conditions in artificial nutritive media were determined for a defined avirulent strain of treponema hyodysenteriae and for four field strains of treponemas in pigs with clinical dysentery. the treponemas were isolated with the use of milliporous filters with pores of 0.3 micrometer in diameter, which were located on the surface of blood agar. no significant difference in the influence of equine, bovine or sheep blood on the growth of treponemas was determined. the commercial amo ...1979105448
[effect of feeding pelleted feed on the level of volatile fatty acids in the digestive tract of sheep].in an experiment with wethers we investigated the effect of complete pelleted feed ration on the concentration of volatile fatty acids in the rumen and intestinal tract. the animals consumed daily 1,300 g of dry matter of a diet which contained 41.81% of meadow hay, 25.28% of barley, 15.37% of sawdust, 14.98% of molasses, 1.32% of urea and 1.24% of a vitamin-mineral supplement for a period of six months. the pelleted diet increased the total amount of volatile fatty acids, the content of propion ...1979105451
[b. cereus count in meat and dairy food products].studies were carried out to establish the contamination of some meat and dairy food products with b. cereus. a total of 48 heat-treated sausages (32 perishable and 16 durable) and 64 batches of pasteurized milk were sampled. it was found that 25 per cent of investigated sausage samples contained b. cereus. perishables proved to a considerable extent more frequently contaminated (33.3 per cent). the count of b. cereus in such products ranged from 10(1) to 10(3) per g. however, pasteurized milk wa ...1978105462
[lysozyme concentration in the loose connective tissue of animals with different species sensitivity to brucellosis].lysozyme content was determined in the subcutaneous cellular tissue of guinea pigs and albino rats differing by species resistance to brucella infection. experiments were conducted on intact animals and those inoculated subcutaneously with live br. abortus 19-ba vaccine. connective tissue was taken from the site of the vaccine administration and from the contralateral side (control). observations showed connective tissue of the animals highly sensitive to brucella infection to be exceedingly poo ...1978105522
thermostable antigens from the bone marrow of several animal species.studies on bone marrow extracts from ten animal species have revealed the presence of thermostable antigens. similar antigens were found in other organs as shown by immunodiffusion and cross-reactivity was demonstrated between the thermostable proteins from horse and dog and between cow and sheep proteins. using an immunoenzymatic reaction with glucose oxidase as the marker, the thermostable antigens were localized in the blood and bone marrow polymorphonuclear leukocytes and the myeloid cell li ...1978105786
cryopreservation of antibody-coated human erythrocytes. 1978105832
insulin and glucagon binding and degradation by kidney cell membranes.the binding and degradation of insulin and glucagon to kidney cell membranes was examined. time- and temperature-dependent specific binding of [125i]iodo-insulin to kidney cell membranes was demonstrated. the membranes also degraded insulin in a time-, temperature-, and protein concentration-dependent manner. the apparent km of the degradation was 2.7 x 10(-7) m. glucagon degradation by the kidney membranes was extremely active. per milligram of protein the kidney membrane was over 20 times as a ...1978105882
effect of continuously increasing concentrations of plasma ketone bodies on the uptake and oxidation of glucose by muscle in man.muscle metabolism in man was studied by measuring the arterial and deepvenous concentrations of glucose, lactate, pyruvate, free fatty acids, beta-hydroxybutyrate and aceacetoacetate, and forearm blood flow. after the subjects had fasted overnight, their arterial free fatty acid and ketone levels rose continuously during a period of 90 min, leading to increased ketone body uptake by muscle. hence, for each subject, a relation was obtained between arterial concentrations and arterial-deepvenous d ...1978105915
the effect of 60-day hypokinesia on submaxillary, stomach, small intestine glands and on gastric secretion. 1978106408
[calcium kinetics of myocardium in experimental hypercalcemia and isoproterenol lesions--ultrastructural and roentgen microanalytical findings]. 1978106573
the role of catecholamines in clinical medicine.the sympathetic nervous system is of major importance for the regulation of several physiological functions. drugs which inhibit the actions of catecholamines and adrenergic drugs are used in the treatment of many clinical disorders. the potential role of catecholamines in a number of human diseases has, however, until recent years been studied to a limited extent only due to lack of methods for quantitation of sympathetic nervous activity. after the development of enzymatic isotope-derivative a ...1979106629
thyroid function studies in the nephrotic serum and urinary thyroxine (t4), triiodothyronine (t3), and thyroxine-binding globulin (tbg) as well as serum free t4, thyroid-stimulating hormone (tsh), and t3 resin uptake (t3ru) were measured in seven patients with the nephrotic syndrome. the nephrotic syndrome was defined by proteinuria exceeding 3 g/24 h. all patients were clinically euthyroid. most values for total serum t4, free t4, t3, t3ru, tbg, and tsh were within normal limits. however, the mean serum t3 and tbg values were sig ...1979106751
carbonic anhydrase of human endometrium: biochemical & clinical aspects. 1978107115
chronic administration of chenodeoxycholic acid increases cholesterol saturation in bile in the dog.six dogs were given chenodeoxycholic acid, 200 mg/day for 15 days; gallbladder and hepatic bile samples were taken and biliary bile acids, phospholipids and cholesterol were compared to those of fourteen control dogs. it was found that: (1) the cholesterol saturation index of gallbladder bile was higher in treated dogs (0.12 +/- 0.06; m +/- sd) than in controls (0.07 +/- 0.01; p less than 0.05); the proportion of chenodeoxycholic acid in gallbladder bile was higher in treated dogs (27.8 +/- 12.5 ...1979110595
elevation of blood lead concentration by confinement in the rhesus monkey.rhesus monkeys were exposed to lead (pb) acetate under various regimens during the first 12 months of life. at 30 months of age, these animals and unexposed controls were confined to an unfamiliar experimental chamber for one week. serum cortisol concentration and pb concentration in whole blood (pbb) were measured prior to, during, and after this confinement. cortisol concentrations rose 60--90% within 2 hours of confinement, and declined to baseline levels after 98 hours of confinement. mean b ...1979114260
immunization against experimental trypanosoma cruzi infection: inherent difficulties of a uniform comparative evaluation of antigenic preparations. 1979120626
human cardiac myosin atpase and light subunits. a comparative study.myosin was extracted from normal human hearts (autopsy material) and compared to that of pig heart and rabbit white skeletal muscle. myosin light subunits were isolated by a preparative urea gel electrophoresis. these subunits were shown by urea and sodium dodecylsulfate gel electrophoresis to be only slightly affected by the time lapse between death and the beginning of myosin extraction. this was also true for myosin atpases. the ca-2+-activated atpases of pig and human heart myosins have the ...1975124584
comparative ultrastructure and blood-brain barrier in diapause and nondiapause larvae of the european corn borer ostrinia nubilalis (hübner).ultrastructural examination of diapause and nondiapause larval brains of the european corn borer disclosed anatomical differences that may be related to the insect's "blood-barrier". the perineurial type i cells are quite closely appressed in the diapause brain, but thrown into extensive folds with large intercellular spaces in the nondiapause brain. the perineurial type ii cells of diapause and nondiapause larvae are basically similar in general ultrastructure, and most likely form the basis fo ...1975126807
amino-acid sequence of the cyanogen-bromide fragment from human plasminogen that forms the linkage between the plasmin chains.the complete amino acid sequence of a cyanogen bromide fragment (122 residues) obtained from plasminogen is described. this fragment forms the overlap between heavy (a) and light (b) chains of human plasmin. the particular arginyl-valyl bond cleaved in the second step of the activation process is shown to be arg98-val99 in this fragment. this site is not very similar to the one in the nh2-terminal part of the molecule (arg68-met69). remarkable homologies with the 'triple loops' ('kringle structu ...1975126863
an ultrastructural study of neuromuscular spindles in normal mice: with reference to mice and man infected with mycobacterium leprae.mycobacterium leprae have been found within muscle spindles in mice, using electron microscopy, and in man, using light microscopy. their mode of entry clearly is important. it may be via capsular cells, capillaries or nerves. for this reason muscle spindles from normal mice were studied by electron microscopy with special reference to the capsule and the relationship of it with capillaries and nerves, as well as details of the intrafusal fibres and capsular space. a fenestrated capillary was fo ...1975126981
characterization of 5.8s ribosomal ribonucleic acid in neurospora crassa.neurospora crassa ribosomes contain a species of ribonucleic acid (rna) of molecular weight 54,000, similar to 5.8s ribosomal rna previously described for other eukaryotic organisms. the 5.8s rna from n. crassa was found to be released by heat treatment at 60 c from 25s ribosomal rna but not from 18s ribosomal rna. the base composition of n. crassa 5.8s rna was similar to that of 5.8s rna from saccharomyces cerevisiae, but differed from animal 5.8s rna. during the course of this study, it was di ...1975126993
[muscular clearance after administration of phenoxybenzamine in shock].the authors made an experimental investigation in the dog concerning the evolution of the muscular clearance of phenoxybenzamine in the course of de-shocking procedures. the animals were shocked by hemorrhage and were treated by perfusion of volemic mass and administration of phenoxybenzamine. this substance was flowed in paralell with the hemodynamic parameters and the rhythm of the diuresis. it was noted that the direction and the amplitude of changes concerning the half-life of na131i do not ...1975127228
[experiments and theoretical cell kinetic calculations on the problem of in vivo synchronization with vincristine in l 1210 ascites tumor cells and crypt cells of the mouse (author's transl)].experiments are described on synchronizing l 1210 ascites tumor cells and jejunal crypt cells of the mouse with vincristine. in both cases the percentage of mitoses increases to a peak value 4 hrs after application of vincristine and decreases during the following 8 hrs ("releasing time") to the level of the normal mitotic index. following this first mitotic peak no futher peak of neither the mitotic nor the labeling index was observed during 48 hrs in l 1210 ascites tumor cells and during 38 hr ...1975127443
[veterinary-sanitary measures on turkey farms]. 1975129930
[economic effectiveness of veterinary measures in swine balantidiasis]. 1976130713
studies on in vitro carcinogenesis. i. autoradiographic and biochemical study on the incorporation of labelled methylcholantrene into mouse embryonic cells.the incorporation of radioactive labelled methylcholanthrene into cba/t6t6 mouse embryonic fibroblast cultures was studied by using light and electronmicroscopic autoradiographic and also liquid scintillation counting techniques. during 24 hrs treatment time at the applied methylcholanthrene concentrations (0.01-2.5 mug/ml) the proportion of cells labelled with 3h-methylcholanthrene and the number of grains above the cells showed a relationship with the duration of treatment and also with the do ...1976130740
treponemal antigen in immunopathogenesis of syphilitic glomerulonephritis.a patient with syphilitic glomerulonephritis had a renal biopsy and was treated for secondary syphilis. light, electron, and immunofluorescence microscopic studies revealed an acute proliferative glomerulonephritis with subepithelial, intramembranous, and subendothelial immune complex deposits containing igg, iga, igm, c4, and c3. similar local deposits containing predominantly igm were noted in areas of mesangial proliferation. indirect fluorescent antibody studies employing rabbit treponemal a ...1976130804
ossification in cancer of the stomach: an experimental rats with nitrosoguanidine induced carcinomas of the gastric stump, heterotopic ossifications are found freqently. the following stages of differentiation during the desmal ossification in the stump carcinomas are demonstrated: 1. osteoblasts, 2. osteoid, 3. woven bone, 4. lamellar bone. --the islands of metaplastic bone cells are predominantly located in the invasive marginal zone of the carcinoma of the gastric stump. the histology of the heterotopic ossification in the gastric stump of the ...1976132787
effects of exercise training and exhaustion on 45ca uptake by rat skeletal muscle mitochondria and sarcoplasmic reticulum.mitochondrial and sarcoplasmic reticular 45ca2+ uptake and ca2+-atpase activity were determined in skeletal muscle from exercise trained and non-trained rats at rest or following short-term exhaustive exercise. in trained rats exercised to exhaustion, mitochondrial 45ca2+ uptake was significantly depressed when compared to non-trained rats at rest. ca2+-atpase activity of sarcoplasmic reticulum from trained rats exercised to exhaustion was significantly increased as compared to trained rats at r ...1976134437
allergic reaction to allopurinol with cross-reactivity to oxypurinol.a 25-year-old white man with gout and nephropathy and with a previous reaction to allopurinol was given a trial dose of oxypurinol. he developed malaise, a generalized erythematous reaction with edema, pruritus, and emesis; this was clinically identical to the reaction he experienced with allopurinol. when the patient's lymphocytes were exposed in vitro to oxypurinol and allopurinol, increased dna synthesis was observed, suggesting an immunologic basis for the reaction. this patient indicates th ...1976134655
[influence of microflora on the metabolism of l-threonine at the level of the rat digestive tract].intestinal tissues are found to incorporate l threonine 14cu at faster rate in the absence of microflora. most of the radioactivity of the digestive contents occurs in the tca soluble compounds, except in the coecum and large intestine of the conventional rats where the microflora synthetized its own proteins from the labelled digestive material. free threonine is the main labelled soluble compound in the conventional rats digestive contents. in the other hand, in the germfree rats digestive con ...1976134804
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