| demonstration of an alpha2-macroglobulin receptor in human fibroblasts, absent in tumor-derived cell lines. | | 1979 | 87392 |
| alpha-fetoprotein as a carrier protein in plasma and its bilirubin-binding ability. | the bilirubin-binding ability of human alpha-fetoproteins, which were purified from fetal cord serum and from ascites fluid of a hepatoma-bearing patient, was examined by the difference spectrum and the jacobsen peroxidase methods. the difference spectrum observed as a result of the specific binding of bilirubin to alpha-fetoprotein had a maximum at 482 nm, and this pattern was quite similar to that observed for serum albumin. the result obtained by the difference spectrum method showed that 1 m ... | 1979 | 89900 |
| projections of auditory nerve in the cat as seen by anterograde transport methods. | | 1979 | 91142 |
| transcription and translation in a pleiotropic streptomycin-resistant mutant of escherichia coli. | the role of the ribosomal protein s12 (streptomycin protein) in ribosome function and in other metabolic processes in the cell has been investigated. a spontaneous streptomycin-resistant strain of escherichia coli (sm3) carrying a mutation in the rpsl gene is deficient in its ability to induce the synthesis of the enzyme bets-galactosidase. it was demonstrated that the reduced rate of enzyme synthesis results from deficiencies in both the transcription of the lactose operon and translation of th ... | 1979 | 104958 |
| a new principle of injectable depot contraceptives. i drug selection and studies in monkeys. | the aim of the investigations was to develop a long-acting depot contraceptive on the basis of norethisterone or levonorgestrel. disappointing results with levonorgestrel nonanoate and levonorgestrel undecylate showed that elongation of the fatty acid, esterified with the steroid, decreased the bioavailability of the latter due to incomplete hydrolysis of the ester. therefore, several new compounds were synthesized which contained a bifunctional molecule between the steroid and the fatty acid. i ... | 1979 | 110513 |
| crosslinking studies on the ca2+, mg2+-activated atpase of escherichia coli. | crosslinking of membrane proteins of escherichia coli with dithiobis (succinimidyl propionate) (dsp) resulted in loss of several enzyme activities including the ca2+, mg2+-activated atpase. this enzyme was crosslinked by dsp to the membrane and was not released by dialysis at low ionic strength in the absence of dithiothreitol which could cleave the crosslinking group. dsp inactivated both phosphohydrolase and coupling activities of the solubilized atpase. loss of hydrolytic activity could be co ... | 1975 | 127090 |
| dissociated cultures of newborn mouse brain. i. metabolism of sulfated lipids and mucopolysaccharides. | the metabolism of 35so4-sulfated lipids and mucopolysaccharides was studied in dissociated brain cell cultures from newborn albino mouse brains. the cultures were maintained under an atmosphere of 40% o2 and 5% co2 in apparent good health up to 30 days. early morphological examination of the dissociated cells demonstrated an initial partial reaggregation of the cells, which later settled and became confluent bilayered cultures. cell proliferation measured by dna and protein determination, morpho ... | 1975 | 128706 |
| the crystal structure of bonito (katsuo) ferrocytochrome c at 2.3 a resolution. ii. structure and function. | the structure analysis of bonito heart ferrocytochrome c was carried out at 2.3 a resolution by x-ray diffraction, and a kendrew-type skeletal model was built up. this molecule has an overall egg shape, 35 a in height, 30 a in width and 23 a in thickness; the 5th ligand of the heme iron atom is the n-epsilon atom of the his-18 imidazole ring and the 6th is the met-80 sulfur atom. distinct alpha-helix regions are found between the n-terminus and reside 11, between 60 and 69, and between 90 and th ... | 1975 | 166072 |
| isoprenaline-induced release of cyclic adenosine 3',5'-monophosphate by the left ventricle in the anaesthetized intact dog. | the ability of the heart to release cyclic adenosine 3',5'-monophosphate (cyclic amp) in the coronary venous blood was examined in closed-chest anaesthetized dogs before and during an infusion of isoprenaline. before the infusion, no difference was observed between the cyclic amp plasma levels in the aortic and coronary sinus blood. during the infusion, significant coronary veno-arterial differences in nucleotide concentration were observed despite a considerable elevation of the cyclic amp plas ... | 1977 | 201223 |
| oxidation of protein sulfhydryls by products of peroxidase-catalyzed oxidation of thiocyanate ion. | | 1978 | 204336 |
| stereo-specific synthesis of 3-trifluoromethylcephalosporin derivative by microbial acylase. | cephalosporin acylase (ec 3.5.1.11) obtained from kluyvera citrophila atcc 21285 was found to catalyze synthesis of 7-[2-(2-thienyl)acetamido]-3-trifluoromethyl-3-cephem-4-carboxylic acid from methyl thienylacetate and dl-7-amino-3-trifluoromethyl-3-cephem-4-carboxylic acid. the enzymatically-synthesized compound showed [alpha]25 d + 42.7 degrees (c 0.058, meoh) and its biological activity was about twice as much as that of racemic 7-[2-(2-thienyl)acetamidol]-3-trifluoromethyl-3-cephem-4-carboxy ... | 1979 | 393680 |
| [a simple method for the determination of pcb in human milk and blood using an improved essential oil distillator (author's transl)]. | | 1977 | 404452 |
| penicillin acylase mutants with altered site-directed activity from kluyvera citrophila. | oligonucleotide-directed mutagenesis has been used to obtain specific changes in the penicillin acylase gene from kluyvera citrophila. wild-type and mutant proteins were purified and the kinetic constants for different substrates were determined. mutations in met168 highly decreased the specificity constant of the enzyme for penicillin g, penicillin v and phenylacetyl-4-aminobenzoic acid and the catalytic constant kcat for phenylacetyl-4-aminobenzoic acid. likewise, the phenylmethylsulphonyl-flu ... | 1990 | 1366784 |
| changing glycine 21 for glutamic acid in the beta-subunit of penicillin g acylase from kluyvera citrophila prevents protein maturation. | active penicillin acylase from kluyvera citrophila strain atcc 21,285 consists of two different alpha- and beta-subunits derived from a single precursor by post-translational processing. using the chemical mutagen hydroxylamine we have treated plasmid pykd59 containing the active penicillin acylase gene (pga) from k. citrophila and have generated different point mutant penicillin acylase genes, one producing a maturation deficient precursor. this point mutation has changed the glycine 310 residu ... | 1992 | 1368070 |
| sleep organization and energy expenditure of breast-fed and formula-fed infants. | sleep organization of infants may be influenced by differences in nutrient intakes from human milk and formula. because sleep/awake and sleep stage patterns affect energy expenditure, we hypothesized that differences in sleep organization between breast-fed and formula-fed infants might account in part for differences in energy expenditure between feeding groups. sleep stages and cycling of 4-mo-old breast-fed (n = 10) formula-fed (n = 10) infants were studied with simultaneous measurements of e ... | 1992 | 1480450 |
| chemical modification of serine at the active site of penicillin acylase from kluyvera citrophila. | the site of reaction of penicillin acylase from kluyvera citrophila with the potent inhibitor phenylmethanesulphonyl fluoride was investigated by incubating the inactivated enzyme with thioacetic acid to convert the side chain of the putative active-site serine residue to that of cysteine. the protein product contained one thiol group, which was reactive towards 2,2'-dipyridyl disulphide and iodoacetic acid. carboxymethylcysteine was identified as the n-terminal residue of the beta-subunit of th ... | 1991 | 1764029 |
| thermodynamic profiles of penicillin g hydrolysis catalyzed by wild-type and met----ala168 mutant penicillin acylases from kluyvera citrophila. | the met-168 residue in penicillin acylase from kluyvera citrophila was changed to ala by oligonucleotide site-directed mutagenesis. the ala-168 mutant exhibited different substrate specificity than wild-type and enhanced thermal stability. the thermodynamic profiles for penicillin g hydrolysis catalyzed by both enzymes were obtained from the temperature dependence of the steady-state kinetic parameters km and kcat. the high values of enthalpy and entropy of activation determined for the binding ... | 1990 | 2306469 |
| the human calbindin 27-kda gene: structural organization of the 5' and 3' regions, chromosomal assignment, and restriction fragment length polymorphism. | the 5' and 3' regions of the human gene coding for calbindin 27 kda were cloned and sequenced. structural features of the 5' region included the presence of an alu repeat and two elements regularly associated with eukaryotic promoters: an alternating purine-pyrimidine element and a homopurine-homopyrimidine box. the 3' region contained a second alu family member and a degenerate 1.4-kb l1 repeat. a comparison with the chicken promoter was made in order to define regions conserved in evolution an ... | 1989 | 2565876 |
| complete nucleotide sequence of the penicillin acylase gene from kluyvera citrophila. | the penicillin acylase (pac) from kluyvera citrophila atcc21285 has been purified to homogeneity and found to be composed of two non-identical subunits of 23 and 62 kda, in contrast with the previous findings [shimizu et al., agr. biol. chem. 39 (1975) 1655-1661]. the nucleotide (nt) sequence of the k. citrophila pac gene contained in the 3-kb pvui-hindiii fragment of pkap1 [garcía and buesa, j. biotechnol. 3 (1986) 187-195] has been determined, showing that it encodes a protein of 844 amino aci ... | 1986 | 3032748 |
| [primary leiomyosarcoma of the pulmonary artery. apropos of a case]. | a case of leiomyosarcoma of the pulmonary artery in a 64-year old man without previous cardiovascular disease is reported. the clinical picture, which comprised episodes of paroxysmal dyspnoea associated with acute cor pulmonale, suggested pulmonary embolism. radioisotope perfusion study and pulmonary angiography seemed to confirm this diagnosis, but no improvement was obtained with a prolonged thrombolytic treatment. the presence of a median mass at ct led to exploratory thoracotomy and to the ... | 1987 | 3122693 |
| phospholipase c activates protein kinase c and induces monocytic differentiation of hl-60 cells. | phospholipase c (plc)-mediated hydrolysis of membrane phospholipids results in the production of diacylglycerol, inositol phosphates, and choline metabolites. inositol triphosphate increases calcium levels, while diacylglycerol activates protein kinase c. the present studies demonstrate that exogenous plc generates inositol phosphates, releases choline metabolites, and activates protein kinase c in human hl-60 promyelocytic leukemia cells. plc also induced monocytic differentiation of hl-60 cell ... | 1988 | 3165300 |
| experimental infection of sheep and goats with muellerius capillaris (protostrongylidae, nematoda). | | 1988 | 3188728 |
| the unborn patient. | | 1988 | 3347563 |
| a kluyvera cryocrescens strain from a gall-bladder infection. | the isolation and the identification of a pure-culture kluyvera cryocrescens strain in a gall-bladder pus specimen from a 76-year-old woman with acute cholecystitis is described. this is the first reported recovery of a k. cryocrescens strain from such a sample. | 1988 | 3356228 |
| differentiation of kluyvera cryocrescens from kluyvera ascorbata by irgasan susceptibility testing. | from the faeces of a diarrhoeic patient, we isolated a strain of kluyvera ascorbata on cefsulodin-irgasan-novobiocin (cin) agar, a highly selective medium used for yersinia enterocolitica. since no selective media have been described for kluyvera spp., we tested 5 strains each of k. ascorbata and k. cryocrescens for their ability to grow on cin agar. k. ascorbata exhibited an inhibition on cin of less than 10% cfu compared to blood agar, whereas k. cryocrescens did not grow at all even with inoc ... | 1986 | 3426156 |
| alternative protein products with different carboxyl termini from a single class i gene, h-2kb. | in addition to mrna encoding the canonical form of the murine class i antigen h-2kb (348 amino acid residues), mrna that would encode a shortened form of h-2kb (missing 9 amino acids from the c-terminus) has been identified in c57bl/6 spleen cells by rnase-protection studies. the alternative transcripts of h-2kb arise through the use of different ag acceptor splice sites for exon viii. the existence of a shortened h-2kb protein was demonstrated by sequential immunoprecipitation. lysates of splee ... | 1986 | 3461476 |
| protostrongylus rufescens: a cytogenetic study. | the diploid chromosome number of protostrongylus rufescens is 2n = 11 for males and 2n = 12 for females. so, the sex determinism mechanism is xo/xx. the study of the genetic behaviour of this species has been made. in diakinesis stage the bivalents show typical tetrads with cross, phi, and lineal configurations. the division of the sexual chromosome is prereductional for the first meiotic division. | 1987 | 3571924 |
| effects of peptide yy on gallbladder motility. | the effects of peptide yy (pyy) on cholecystokinin-stimulated gallbladder contraction were investigated in the prairie dog model. twelve animals underwent laparotomy with catheter placement into the gallbladder and common bile duct (vent). the gallbladder was continuously perfused with [14c]polyethylene glycol-labeled lactated ringer at 0.03 ml/min, and vent effluent was collected at 2.5-min intervals. all animals received 20 min of intravenous infusion of cholecystokinin octapeptide (cck-op), 2 ... | 1987 | 3591942 |
| broviac catheter infection with kluyvera cryocrescens: a case report. | kluyvera cryocrescens was isolated from a blood culture drawn from a patient with a broviac catheter-related infection. k. cryocrescens has been considered an opportunistic pathogen but has not previously been associated with central venous catheter infections. | 1987 | 3597755 |
| social, economic, and surgical anatomy of a drug-related abscess. | the "letting of pus" has been a surgical triumph throughout medical history. this study was initiated to expand our knowledge of the etiologic, economic, and surgical aspects of an abscess. the records of 651 patients undergoing intraoperative incision and drainage of an abscess over a 12-month interval were analyzed. the abscesses were due to injection of illicit street drugs in 421 patients (64.7%), perirectal, pilonidal or sweat gland inflammation in 118 patients (18%), complications of diabe ... | 1986 | 3729176 |
| sweat gland response to local heating during sleep in man. | in order to assess whether the fluctuations in the sweating response occurring during sleep are related to changes in central drive or in peripheral sweat gland reactivity, 4 healthy male subjects spent 6 non-consecutive nights in a climatic chamber. air temperature was 25 degrees c, dew-point temperature was 10 degrees c and air velocity was 0.3 m x s-1, while wall temperature was either 38 degrees c, 46 degrees c or 48.7 degrees c giving 3 levels of operative temperature (to = 30, 33 or 34 deg ... | 1986 | 3795118 |
| determination of nanogram quantities of vanadium in biological material by isotope dilution thermal ionization mass spectrometry with ion counting detection. | | 1985 | 3907412 |
| day to day variations in morphology and duration of fragmented ventricular potentials during the late post-myocardial infarction phase in conscious dogs. | the relationship between the inducibility of ventricular tachyarrhythmias and the presence of fractionated epicardial ventricular electrograms ('late potentials') was studied daily between days 3 and 8 after experimental myocardial infarction in 15 conscious dogs. before each programmed stimulation, epicardial infarct zone electrograms were recorded from implanted 'composite' electrodes during sinus rhythm. there was considerable daily variation in the morphology and duration of delayed ventricu ... | 1985 | 4054137 |
| continuous splitting off of phosphate from phosvitins by phosphoprotein phosphatase. | | 1969 | 4309716 |
| test method for determining the viricidal activity of disinfectants against vesicular stomatitis virus. | a test method using embryonating chicken eggs for the evaluation of several types of disinfectants as viricides against vesicular stomatitis virus was developed which gave specific, reproducible results. | 1970 | 4313316 |
| catabolism of hamamelose. the anaerobic dissimilation of d-hamamelose by kluyvera citrophila 627. | | 1974 | 4452358 |
| [the synthesis of rat liver and kidney lysosomes (author's transl)]. | | 1974 | 4594653 |
| stimulation of cell-free polypeptide synthesis by a protein fraction extracted from chick oviduct polyribosomes. | | 1972 | 4622202 |
| giardia intestinalis. a prospective investigation of the prevalence in denmark. | | 1973 | 4697076 |
| [supplement to the study of the mechanism of electrocardiographic alterations in meningeal hemorrhage (experimental study)]. | | 1969 | 4975492 |
| enzymatic synthesis of d(-)-alpha-aminobenzylpenicillin by kluyvera citrophila. | | 1971 | 5581352 |
| purification and properties of hamamelosekinase. | hamamelosekinase (atp:hamamelose 2(1)-phosphotransferase) was purified from a crude extract of kluyvera citrophila 627 (enterobacteriaeceae) which has been grown on d-hamamelose. ammonium-sulfate fractionation and twofold chromatography on deae-cellulose resulted in a 51-fold purification of the enzyme. neither glucosekinase nor significant atpase activity could be detected in the pure preparation. besides d-hamamelose only d-hamamelitol was utilized as a substrate; however, the latter was phosp ... | 1980 | 6249593 |
| kluyvera, a new (redefined) genus in the family enterobacteriaceae: identification of kluyvera ascorbata sp. nov. and kluyvera cryocrescens sp. nov. in clinical specimens. | kluyvera is proposed as a new genus for the group of organisms formerly known as enteric group 8 (synonym = api group 1). strains of kluyvera share the properties of most members of the family enterobacteriaceae: they are gram-negative rods, motile with peritrichous flagella, catalase positive, and oxidase negative; they grow on macconkey agar, ferment d-glucose with the production of acid and gas, and are susceptible to many antibiotics. strains are usually indole positive, methyl red positive, ... | 1981 | 7240403 |
| new insights on the specificity of penicillin acylase. | in contrast with the general thought that penicillin g acylases (pgas) were only able to hydrolyse amides or esters of higly hydrophobic acids, we have demonstrated that the pga from kluyvera citrophila catalysed the hydrolysis of 4-nitrophenyl esters of acetic, propionic, butyric and valeric acids. values of kcat. and kcat./km were greatest for the first compound and less than values for benzylpenicillin by factors of 30 and 7, respectively. 4-nitrophenyl acetate was hydrolysed faster than 2-ni ... | 1995 | 7826381 |
| changing the substrate specificity of penicillin g acylase from kluyvera citrophila through selective pressure. | escherichia coli (mut, mutd, leu-) cells transformed with plasmid pykd59 harbouring the pac gene encoding penicillin acylase (pa) from kluyvera citrophila atcc 21285 were exposed to environmental conditions that made expression of this enzyme essential for growth. under these conditions, spontaneous mutants were isolated that used adipyl-l-leucine as the sole source of l-leucine. dna sequencing of the mutant pac genes identified a transversion mutation of thymine to guanine at position 1163. thi ... | 1994 | 7980457 |
| ph studies to elucidate the chemical mechanism of penicillin acylase from kluyvera citrophila. | the variation with ph of the kinetic parameters of penicillin acylase from kluyvera citrophila has been used to gain information about the chemical mechanism of the reaction catalysed by the enzyme. the ph-dependence of log (v/km) for penicillin g showed that a group with a pk value over 4.7 must be deprotonated and that a group with a pk value over 9.7 must be protonated in the free enzyme for activity. the solvent perturbation and temperature studies indicated that these groups are respectivel ... | 1993 | 8338640 |
| characterization of an escherichia coli aromatic hydroxylase with a broad substrate range. | the hpab gene encoding an aromatic hydroxylase of escherichia coli atcc 11105, a penicillin g acylase-producing strain, has been cloned and expressed in e. coli k-12. this gene was located near the paca gene coding for penicillin g acylase. the hydroxylase has a molecular mass of 59,000 da, uses nadh as a cosubstrate, and was tentatively classified as a 4-hydroxyphenylacetic acid hydroxylase, albeit it exhibited a rather broad substrate specificity acting on different monohydric and dihydric phe ... | 1993 | 8458860 |
| inactivation of penicillin acylase from kluyvera citrophila by n-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline: a case of time-dependent non-covalent enzyme inhibition. | penicillin acylase (pa) from kluyvera citrophila was inhibited by n-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline (eedq), a specific carboxy-group-reactive reagent. enzyme activity progressively decreased to a residual value depending on eedq concentration. neither enzymic nor non-enzymic decomposition of eedq is concomitant with pa inactivation. moreover, enzyme re-activation is achieved by chromatographic removal of eedq, ph increase or displacement of the reagent with penicillin g. it was then ... | 1993 | 8489517 |
| rapid burst kinetics in the hydrolysis of 4-nitrophenyl acetate by penicillin g acylase from kluyvera citrophila. effects of mutation f360v on rate constants for acylation and de-acylation. | the kinetics of release of 4-nitrophenol were followed by stopped-flow spectrophotometry with two 4-nitrophenyl ester substrates of penicillin g acylase from kluyvera citrophila. with the ester of acetic acid, but not of propionic acid, there was a pre-steady-state exponential phase, the kinetics of which were inhibited by phenylacetic acid (a product of hydrolysis of specific substrates) to the extent predicted from ki values. this was interpreted as deriving from rapid formation (73 mm-1.s-1) ... | 1996 | 8687381 |
| a case of kluyvera cryocrescens peritonitis in a capd patient. | | 1996 | 8761550 |
| [utility of pyrrolidonyl-arylamidase detection for typing enterobacteriaceae and non-fermenting gram-negative bacteria]. | detection of pyrrolidonyl-aryl-amidase activity (pyr) is an important tool to identify gram-positive cocci, such as staphylococci, enterococci, streptococci, and other related genera. however, only few studies evaluating its usefulness with gram-negative rods have been published. thus, a prospective study including 542 and 215 unique clinical isolates of enterobacteriaceae and non-fermentative gram-negative rods, respectively, was undertaken. strains were identified by conventional methods. pyr ... | 1995 | 8850133 |
| identification of the pac promoter from kluyvera citrophila. | the nucleotide sequence of the 5'-terminal region of the pac gene encoding the penicillin g acylase from kluyvera citrophila atcc 21285 has been determined. the transcriptional start site has been identified by primer extension analysis in a different position to that previously found for the homologous pac gene of escherichia coli w atcc 11105. two nucleotide changes in the -35 box appear to be responsible of the promoter displacement in k. citrophila. a putative upstream promoter element (a+t- ... | 1997 | 9198278 |
| kluyvera bacteriophage kvp1: a new member of the podoviridae family phylogenetically related to the coliphage t7. | a dna containing bacteriophage, kvp1, was isolated from the water of a very polluted river, the matanza river, near the central district of buenos aires city. this bacteriophage infects bacteria belonging to the kluyvera cryocrescens species (strain 21 g) isolated from the same river. kvp1 is a lytic bacteriophage and its propagation characteristics are: burst size 30, latent period 13 min and rise period 10 min. morphologically, kvp1 is a small icosahedral bacteriophage, 59.1 nm in diameter, wh ... | 1997 | 9381794 |
| kluyvera cryocrescens finger infection: case report and review of eighteen kluyvera infections in human beings. | we report a case of soft tissue infection with kluyvera cryocrescens and a critical review of kluyvera infections. a 31-year-old diabetic man used a new chemical for stripping the floor with his bare hands. two days later he developed a blister on a finger which progressed to tenosynovitis in spite of intravenous nafcillin therapy. after 11 days culture and sensitivity results dictated treatment with intravenous ticarcillin/clavulanic acid. the wound was debrided twice, and later a skin flap was ... | 1998 | 9884842 |
| [kluyvera cryocrescens: a positive urine culture in a young girl with persistent proteinuria]. | kluyvera genus usually shows two kinds of species: k. ascorbata and k. cryocrescens, dna hybridization let us to differentiate a third group: kluyvera species 3. its diagnosis is quite uncommon and its taxonomy have been recently clarified. we report here a ten years female record with a chronic proteinuria and a positive urine-culture for k. cryocrescens. axetil cefuroxime treatment was absolutely succesful. kluyvera infections are difficult on the whole to joint with some specific clinical fea ... | 1999 | 10464962 |
| the use of chromogenic reference substrates for the kinetic analysis of penicillin acylases. | determination of kinetic parameters of penicillin acylases for phenylacetylated compounds is complicated due to the low k(m) values for these substrates, the lack of a spectroscopic signal, and the strong product inhibition by phenylacetic acid. to overcome these difficulties, a spectrophotometric method was developed, with which kinetic parameters could be determined by measuring the effects on the hydrolysis of the chromogenic reference substrate 2-nitro-5-[(phenylacetyl)amino]benzoic acid (ni ... | 1999 | 10542108 |
| outbreak of nosocomial infections due to extended-spectrum beta-lactamase-producing strains of enteric group 137, a new member of the family enterobacteriaceae closely related to citrobacter farmeri and citrobacter amalonaticus. | a member of the enterobacteriaceae initially identified as kluyvera cryocrescens by the microscan gram-negative combo 13 panel caused an outbreak of nosocomial infections in four patients (pneumonia, n = 2; urinary tract infection, n = 1; wound infection, n = 1) and urinary tract colonization in one patient. when the strains were tested by the enteric reference laboratory of the centers for disease control and prevention, biochemical results were most compatible with yersinia intermedia, kluyver ... | 2000 | 11060050 |
| fluorogenic assay for penicillin g acylase activity. | a simple, highly sensitive, and rapid assay for high-throughput screening of penicillin g acylase-producing bacteria is presented. the method is based on the specific release of fluorescent 7-amino-4-methyl-coumarin through cleavage of phenylacetyl-4-methyl-coumaryl-7-amide by penicillin g acylase. the present method is suitable for screening pure enzymes as well as various penicillin g acylases like those from escherichia coli, proteus rettgeri, and kluyvera citrophila in cell extracts. in addi ... | 2001 | 11355855 |
| further studies on rpos in enterobacteria: identification of rpos in enterobacter cloacae and kluyvera cryocrescens. | rpos, the alternative sigma factor sigma(s), is important for bacterial survival under extreme conditions. many enterobacteria are opportunistic human pathogens and their ability to survive in a changing environment could be an essential step for their virulence. to determine the presence of this gene in enteric bacteria, an escherichia coli rpos probe was constructed and used to detect the presence of this gene in different species. a gene homologous to rpos was found in citrobacter amalonaticu ... | 2001 | 11491080 |
| characterization of a chromosomally encoded extended-spectrum class a beta-lactamase from kluyvera cryocrescens. | a chromosomally located beta-lactamase gene, cloned and expressed in escherichia coli from a reference strain of the enterobacterial species kluyvera cryocrescens, encoded a clavulanic acid-inhibited ambler class a enzyme, kluc-1, with a pi value of 7.4. kluc-1 shared 86% amino acid identity with a subgroup of plasmid-mediated ctx-m-type extended-spectrum beta-lactamases (ctx-m-1, -3, -10, -11, and -12), the most closely related enzymes, and 77% amino acid identity with klua-1 from kluyvera asco ... | 2001 | 11709346 |
| nosocomial outbreak of kluyvera cryocrescens bacteremia. | | 2002 | 11893149 |
| identification of a chromosome-borne expanded-spectrum class a beta-lactamase from erwinia persicina. | from whole-cell dna of an enterobacterial erwinia persicina reference strain that displayed a penicillinase-related antibiotic-resistant phenotype, a beta-lactamase gene was cloned and expressed in escherichia coli. it encoded a clavulanic-acid-inhibited ambler class a beta-lactamase, erp-1, with a pi value of 8.1 and a relative molecular mass of ca. 28 kda. erp-1 shared 45 to 50% amino acid identity with the most closely related enzymes, the chromosomally encoded enzymes from citrobacter koseri ... | 2002 | 12384342 |
| polymorphism in the yclc-rpos region of enterobacteria. | the nucleotide sequence downstream from the rpos gene in enterobacter cloacae and kluyvera cryocrescens contains the slya-pad1-yclc genes. the dna sequence of enterobacter cloacae cetc960 shows a 2.6-kb insertion of unknown origin between rpos and slya. this 2.6-kb sequence has also been detected in species of salmonella and in pseudomonas aeruginosa, but not in the same location. this insertion has been detected in all the enterobacter cloacae clinical strains studied although the size of the r ... | 2003 | 12732965 |
| improving the specific synthetic activity of a penicillin g acylase using dna family shuffling. | penicillin g acylas (pga) of providencia rettgeri (atcc 25599) was evolved using a modified dna family shuffling method. the identity of pga genes from escherichia coli, kluyvera citrophila and providencia rettgeri ranges from 62.5% to 96.9%. the pga genes from above three species were recombined and shuffled to create interspecies pga gene fusion libraries. by substituting assembled chimaeras for corresponding region of petppga, different recombinants were constructed and expressed in e. coli j ... | 2003 | 12796820 |
| cloning, overexpression, and characterization of a novel thermostable penicillin g acylase from achromobacter xylosoxidans: probing the molecular basis for its high thermostability. | the gene encoding a novel penicillin g acylase (pga), designated pgaw, was cloned from achromobacter xylosoxidans and overexpressed in escherichia coli. the pgaw gene contains an open reading frame of 2586 nucleotides. the deduced protein sequence encoded by pgaw has about 50% amino acid identity to several well-characterized pgas, including those of providencia rettgeri, kluyvera cryocrescens, and escherichia coli. biochemical studies showed that the optimal temperature for this novel pga (pga6 ... | 2004 | 15128530 |
| expression, purification, and characterization of his-tagged penicillin g acylase from kluyvera citrophila in escherichia coli. | the dna fragment encoding kluyvera citrophila penicillin g acylase (kcpga) was amplified and cloned into the vector pet28b to obtain a c-terminus his-tagged fusion expression plasmid. the fusion protein kcpga was successfully overexpressed in escherichia coli bl21(de3). the optimal induction concentration of isopropylthio-beta-d-galactoside (iptg) was found to be 5 microm. the fusion protein was purified in a single step by ni-ida affinity chromatograph to a specific activity of 35.3u/mg protein ... | 2004 | 15477078 |
| evidence for the involvement of arginyl residue at the active site of penicillin g acylase from kluyvera citrophila. | penicillin g acylase (pga) is used for the commercial production of semi-synthetic penicillins. it hydrolyses the amide bond in penicillin producing 6-aminopenicillanic acid and phenylacetate. 6-aminopenicillanic acid, having the beta-lactam nucleus, is the parent compound for all semi-synthetic penicillins. penicillin g acylase from kluyvera citrophila was purified and chemically modified to identify the role of arginine in catalysis. modification with 20 mm phenylglyoxal and 50 mm 2,3-butanedi ... | 2004 | 15604805 |
| phylogenetic relationships of the genus kluyvera: transfer of enterobacter intermedius izard et al. 1980 to the genus kluyvera as kluyvera intermedia comb. nov. and reclassification of kluyvera cochleae as a later synonym of k. intermedia. | in order to assess the relationship between the genus kluyvera and other members of the family enterobacteriaceae, the 16s rrna genes of type strains of the recognized kluyvera species, kluyvera georgiana, kluyvera cochleae, kluyvera ascorbata and kluyvera cryocrescens, were sequenced. a comparative phylogenetic analysis based on these 16s rrna gene sequences and those available for strains belonging to several genera of the family enterobacteriaceae showed that members of the genus kluyvera for ... | 2005 | 15653915 |
| natural antimicrobial susceptibility patterns of kluyvera ascorbata and kluyvera cryocrescens strains and review of the clinical efficacy of antimicrobial agents used for the treatment of kluyvera infections. | the natural susceptibility of 58 k. ascorbata and 24 k. cryocrescens strains to 71 antimicrobial agents was investigated. mic values were determined with a microdilution procedure in cation-adjusted mueller hinton broth (for all strains) and isosensitest broth (for some strains). both species were naturally sensitive or of intermediate susceptibility to tetracyclines, aminoglycosides, quinolones, antifolates, chloramphenicol, nitrofurantoin, fosfomycin, aminopenicillins plus beta-lactamase inhib ... | 2005 | 15920899 |
| expression and purification of penicillin g acylase enzymes from four different micro-organisms, and a comparative evaluation of their synthesis/hydrolysis ratios for cephalexin. | several genes for the enzyme penicillin g acylase, as isolated from four different micro-organisms (alcaligenes facaelis, escherichia coli, kluyvera cryocrescens or providencia rettgeri) were modified at their carboxy-termini to include his-tag fusions, then were expressed from the plasmid pet-24a(+) in e. coli jm109(de3) cells. all fusion proteins were next purified to homogeneity in a single step by agar-based co-ida chromatography, and were then evaluated as catalysts for the synthesis of cep ... | 2006 | 16139515 |
| effect of organic cosolvent on kinetic resolution of tert-leucine by penicillin g acylase from kluyvera citrophila. | penicillin g acylase (pga) from kluyvera citrophila immobilized on amberzyml was used for enantioselective hydrolysis of n-phenylacetylated-dl-tert-leucine (n-phac-dl-tle) to produce l-tert-leucine (l-tle). the effects of various organic cosolvents on hydrolysis of n-phac-dl-tle have been investigated in aqueous-cosolvent medium. it was founded that the rate of pga-catalyzed reaction was significantly affected by the presence of 2% (v/v) organic cosolvent concentration. the initial rate fell wit ... | 2006 | 16245080 |
| preparation of optically pure tert-leucine by penicillin g acylase-catalyzed resolution. | penicillin g acylase, from kluyvera citrophila, was used in kinetic resolution of dl-tert-leucine. n-phenylacetylated-dl-tert-leucine, chemically synthesized from dl-tert-leucine, was enantioselctively hydrolyzed by penicillin g acylase to obtain l-tert-leucine, d-tert-leucine was prepared by acid-catalyzed hydrolysis of the remaining substrate. the total yields of d-tert-leucine and l-tert-leucine are 80.6% and 83.1%, respectively. the enantiomeric excess of the two products, d-tert-leucine and ... | 2006 | 16707334 |
| kluyvera co-infection in two solid organ transplant recipients: an emerging pathogen or a colonizer bystander? | kluyvera species are opportunistic, gram-negative bacilli in the family enterobacteriaceae. ordinarily occurring as a commensal, kluyvera have been reported to cause serious infections in immunosuppressed and immunocompetent hosts, causing diarrhea, urinary infections, peritonitis, and cholecystitis. we report kluyvera infections in 2 solid organ transplant recipients. an 18-year-old female with alpha-1 antitrypsin deficiency underwent living donor liver transplantation and presented 6 months la ... | 2007 | 17313481 |
| effects of induction starting time and ca2+ on expression of active penicillin g acylase in escherichia coli. | formation of inclusion bodies is an important obstacle to the production of active recombinant protein in escherichia coli. thus, soluble expression of penicillin g acylase from kluyvera citrophila was investigated in bl21(de3). in this study, the yield of active enzyme was significantly enhanced by the composition of the medium and induction opportunity. when 0.5 mmol/l iptg was added to complex medium at 15 h after incubation, the volumetric and specific activities of penicillin g acylase both ... | 2007 | 17824667 |
| hickman catheter-related bacteremia with kluyvera cryocrescens: a case report. | this report describes a 2-year-old child with neuroectodermal tumor presenting with febrile neutropenia. blood cultures drawn from the peripheral vein and hickman catheter revealed kluyvera cryocrescens growth. the hickman catheter was removed and the patient was successfully treated with cefepime and amikacin. isolation of kluyvera spp. from clinical specimens is rare. this saprophyte microorganism may cause serious central venous catheter infections, especially in immunosuppressed patients. cl ... | 2008 | 18503178 |
| stabilization of heterodimeric enzyme by multipoint covalent immobilization: penicillin g acylase from kluyvera citrophila. | we have developed a strategy for immobilization-stabilization of penicillin g acylase (pga) from kluyvera citrophila by controlled multipoint covalent attachment to agarose-aldehyde gels. this enzyme is composed by two dissimilar subunits noncovalently bound. thus, in this article we establish clear correlations between enzyme stabilization and the multipoint immobilization and/or between enzyme stabilization and the involvement of the two subunits in the attachment of them to the support. we ha ... | 1993 | 18613049 |
| genetic and structural insights into the dissemination potential of the extremely broad-spectrum class a beta-lactamase kpc-2 identified in an escherichia coli strain and an enterobacter cloacae strain isolated from the same patient in france. | two clinical strains of escherichia coli (2138) and enterobacter cloacae (7506) isolated from the same patient in france and showing resistance to extended-spectrum cephalosporins and low susceptibility to imipenem were investigated. both strains harbored the plasmid-contained bla(tem-1) and bla(kpc-2) genes. bla(kluc-2), encoding a mutant of the chromosomal beta-lactamase of kluyvera cryocrescens, was also identified at a plasmid location in e. cloacae 7506, suggesting the isecp1-assisted escap ... | 2008 | 18625772 |
| the genome and proteome of the kluyvera bacteriophage kvp1--another member of the t7-like autographivirinae. | kluyvera, a genus within the family enterobacteriaceae, is an infrequent cause of human infections. bacteriophage kvp1, the only bacteriophage isolated for one of its species, kluyvera cryocrescens, is a member of the viral family podoviridae. | 2008 | 18937848 |
| genetic transformation of midgut bacteria from the red imported fire ant (solenopsis invicta). | in our previous study we isolated 10 bacterial species from fourth-instar larval midguts of the red imported fire ant, solenopsis invicta. here we report the genetic transformation and reintroduction of three species (kluyvera cryocrescens, serratia marcescens, and isolate 38) into the fire ant host. all three species were transformed with the plasmid vector, pzeodsred. high expression levels of dsred were observed and the plasmid is maintained in these bacteria at 37 degrees c in the absence of ... | 2009 | 19159973 |
| antibiotic susceptibility profiles of uncommon bacterial species causing severe infections in italy. | this study presents the results of the italian "severe infections project" involving bacteria that can be considered rare causes of disease. we isolated 30 uncommon human pathogens from a total of 60 strains (1.2% of all the isolates). the most frequent sources of uncommon human pathogens were primary bloodstream infections (48.3%) and pneumonia (20%). species such as comamonas testosteroni, enterococcus hirae, kluyvera ascorbata, kluyvera cryocrescens, leclercia adecarboxylata and ochrobactrum ... | 2009 | 19567344 |
| kluyvera cryocrescens sepsis in a preterm infant. | kluyvera cryocrescens, formerly accepted as a benign saprophytic microorganism, is an opportunistic pathogen and its infection is very rare in humans. this report describes a preterm infant born at 30 weeks of gestational age and successfully treated for k. cryocrescens sepsis in the 3rd week of life. to our knowledge, this is the first case of k. cryocrescens sepsis in a newborn. the potential of k. cryocrescens as a serious pathogen should be recognized especially in patients such as preterm i ... | 2010 | 20495273 |
| one-pot, two-step enzymatic synthesis of amoxicillin by complexing with zn2+. | a one-pot, two-step enzymatic synthesis of amoxicillin from penicillin g, using penicillin acylase, is presented. immobilized penicillin acylase from kluyvera citrophila was selected as the biocatalyst for its good ph stability and selectivity. hydrolysis of penicillin g and synthesis of amoxicillin from the 6-aminopenicillanic acid formed and d-p-hydroxyphenylglycine methyl ester were catalyzed in situ by a single enzyme. zinc ions can react with amoxicillin to form complexes, and the yield of ... | 2010 | 20567815 |
| ethanol production from biodiesel-derived crude glycerol by newly isolated kluyvera cryocrescens. | the rapidly expanding market for biodiesel has increased the supply and reduced the cost of glycerol, making it an attractive sustainable feed stock for the fuel and chemical industry. glycerol-based biorefinery is the microbial fermentation of crude glycerol to produce fuels and chemicals. a major challenge is to obtain microbes tolerant to inhibitors such as salts and organic solvents present in crude glycerol. microbial screening was attempted to isolate novel strain capable of growing on cru ... | 2011 | 21212944 |
| parthenium dermatitis sparing vitiliginous skin. | | 2013 | 3581827 |
| education and the hospital: hospital office promotes health education. | | 2014 | 4119583 |
| resection of a massive arteriovenous malformation of the head and neck. | we report a patient with a massive arteriovenous malformation of the head and neck treated by surgical extirpation of the lesion. arteriography, hypotensive hypothermic anesthesia, and the intraoperative use of the doppler flowmeter were most helpful in this case. | 2003 | 154679 |
| tuberous sclerosis and schizophrenia. | | 2015 | 4648445 |
| [functioning and non-functioning tumors of the insular pancreas. pathology, clinical aspects and therapy]. | | 2013 | 4320215 |
| the histaminase activity of ceruloplasmin. | | 2015 | 4630290 |
| role of mental imagery in free recall of deaf, blind, and normal sunjects. | | 2015 | 4705238 |
| [renal osteodystrophy in children with chronic renal insufficiency]. | | 2012 | 3769023 |
| comparison of segmental pancreatic transplantation with duct obstruction and pancreaticoduodenal transplantation with enteric diversion. | | 2012 | 3313861 |
| a framework for the knowledge-based interpretation of laboratory data in intensive care units using deductive database technology. | in co-operation with the institute of anaesthesiology of the ludwig-maximilians-university in munich a computer-based system for the analysis and interpretation of renal function, fluid and electrolyte metabolism of critical care patients has been developed. this paper focuses on the requirements and implementation aspects of the knowledge-based interpretation for this particular system. objective of the proposed approach is, to transform an enormous--and constantly increasing--amount of raw dat ... | 2006 | 1482854 |
| [melanotic progonoma. report of two cases (author's transl)]. | | 2003 | 182628 |
| [ultrasonic localization for closed renal biopsy]. | | 2004 | 520913 |
| a review of the safety profile of teicoplanin. | safety of teicoplanin has been assessed in 3377 patients treated in europe up to the end of june 1990. one or more adverse events were experienced by 10% of patients. age and teicoplanin dose had no significant effect on the incidence or type of adverse event. in comparative trials the incidence and profile of adverse events to teicoplanin have been similar to those seen with beta-lactam therapy. impaired renal function occurred consistently more frequently with vancomycin therapy than with teic ... | 2006 | 1829079 |
| health checks on patients 75 years and over in nottinghamshire after the new gp contract. | to investigate annual health checks for patients of 75 years and over required by the 1990 contract for general practitioners. | 2006 | 1393076 |
| some findings on the pathology of the motor nerves of the limbs during medical diseases: the possibility of proximal involvement with conservation of distal excitability. | | 2012 | 4103553 |
| epidemiology of allergic contact sensitization. | | 2012 | 3316993 |
| [properties of lak cell activity and lak-generation in pbl isolated from patients with renal cell carcinoma]. | | 2012 | 3266646 |