| a new principle of injectable depot contraceptives. i drug selection and studies in monkeys. | the aim of the investigations was to develop a long-acting depot contraceptive on the basis of norethisterone or levonorgestrel. disappointing results with levonorgestrel nonanoate and levonorgestrel undecylate showed that elongation of the fatty acid, esterified with the steroid, decreased the bioavailability of the latter due to incomplete hydrolysis of the ester. therefore, several new compounds were synthesized which contained a bifunctional molecule between the steroid and the fatty acid. i ... | 1979 | 110513 |
| splenic influence of t cell function: the immunobiology of the inbred hereditarily asplenic mouse. | | 1977 | 194980 |
| [i.b.r (author's transl)]. | the clinical pictures which may be produced by ibr/ipv virus in cattle are briefly reviewed. up to the autumn of 1972, infection occurred only sporadically in the netherlands, no typical cases of ibr having been reported up to the fall of that year. since the winter of 1972-1973 ibr has increasingly been diagnosed, particularly during the winter months. it may, however, be mistaken for other infections of the respiratory tract. the infection apears to be spreading. the clinical features, diagnos ... | 1975 | 168664 |
| deoxyribinucleic acid-binding proteins in virus-transformed cell lines. | the synthesis of proteins with affinity for dna has been studied in clones of a syrian hamster cell line (nil) and subclones of this line transformed by polyoma virus (nil-py) or hamster sarcoma virus (nil-hsv). the results show that the synthesis of dna-binding proteins in nil and in its virus-transformed derivatives nil-py and nil-hsv is very similar in exponentially growing cells, but in dense culture there is a very significant difference in the level of a protein (p8), which is much higher ... | 1975 | 165180 |
| proton translocation in cytochrome-deficient mutants of escherichia coli. | cytochrome-deficient cells of a strain of escherichia coli lacking 5-amino-levulinate synthetase have been used to study proton translocation associated with the reduced nicotinamide adenine dinucleotide (nadh) dehydrogenase region of the electron transport chain. menadione was used as electron acceptor, and mannitol was used as the substrate for the generation of intracellular nadh. the effects of iron deficiency on nadh- and d-lactate-menadione reductase activities were studied in iron-deficie ... | 1979 | 154508 |
| two calcium regulation systems in squid (ommastrephes sloani pacificus) muscle. preparation of calcium-sensitive myosin and troponin-tropomyosin. | the ca-regulatory system in squid mantle muscle was studied. the findings were as follows. (a) squid mantle myosin b (squid myosin b) was ca-sensitive, and its ca-sensitivity was unaffected by addition of a large amount of rabbit skeletal myosin (skeletal myosin) or rabbit skeletal f-actin (skeletal f-actin). (b) squid myosin was prepared from the mantle muscle. it showed a heavy chain component and two light chain components in the sds-gel electrophoretic pattern: the molecular weights of the l ... | 1978 | 153902 |
| transcription and translation in a pleiotropic streptomycin-resistant mutant of escherichia coli. | the role of the ribosomal protein s12 (streptomycin protein) in ribosome function and in other metabolic processes in the cell has been investigated. a spontaneous streptomycin-resistant strain of escherichia coli (sm3) carrying a mutation in the rpsl gene is deficient in its ability to induce the synthesis of the enzyme bets-galactosidase. it was demonstrated that the reduced rate of enzyme synthesis results from deficiencies in both the transcription of the lactose operon and translation of th ... | 1979 | 104958 |
| the importance of pre-treatment of human sera for the titration of hemagglutination-inhibiting antibodies towards bk virus. | | 1979 | 95157 |
| pharmacokinetics of tallysomycin and bleomycin in the beagle dog. | the pharmacokinetics of tallysomycin, a third-generation bleomycin analog, and bleomycin have been determined and compared in the beagle dog. both compounds exhibited biphasic plasma elimination characteristics and were extensively absorbed after in injection. the elimination half-lives of tallysomycin after iv and im administration were 1.51 +/- 0.41 hours and 2.40 +/- 0.667 hours respectively. these values were longer than the comparable iv (1.01 +/- 0.19 hours) and im (1.12 +/- 0.39 hours) el ... | 1979 | 93509 |
| serological cross-reactions of leptospiral lipopolysaccharide (f4) antigen. | the serological specificity of leptospiral lipopolysaccharide (f4) antigen was examined by the technique of passive haemagglutination. f4 extracted from leptospiral serovars representative of several different serogroups showed wide cross reaction between serovars, including numerous one-way (non-reciprocal) reactions. the pattern of cross reaction was different to that of the standard leptospiral classification scheme. | 1979 | 92115 |
| the effect of 5-azacytidine on e. coli dna methylase. | | 1979 | 91371 |
| alpha-fetoprotein as a carrier protein in plasma and its bilirubin-binding ability. | the bilirubin-binding ability of human alpha-fetoproteins, which were purified from fetal cord serum and from ascites fluid of a hepatoma-bearing patient, was examined by the difference spectrum and the jacobsen peroxidase methods. the difference spectrum observed as a result of the specific binding of bilirubin to alpha-fetoprotein had a maximum at 482 nm, and this pattern was quite similar to that observed for serum albumin. the result obtained by the difference spectrum method showed that 1 m ... | 1979 | 89900 |
| [use of herman's method of staining mycobacterium tuberculosis]. | | 1979 | 89264 |
| immune suppression in vivo with antigen-modified syngeneic cells. ii. t cell-mediated nonresponsiveness to fowl gamma-globulin. | intravenous administration of syngeneic spleen cells coupled with the palmitoyl derivative of fowl gammma-globulin (p-f gamma g) results in a profound state of f gamma g-specific tolerance in c57bl/6 mice. administration of p-f gamma g coupled syngeneic cells specifically reduces both the primary and secondary hapten and carrier-specific pfc responses to tnp-f gamma g. since the haptenic response is affected, the tolerance functions at the level of the f gamma g-specific helper t cell. as few as ... | 1979 | 87447 |
| tissular immunoenzymatic detection of hepatic alphafetoprotein in human hepatomas. | 5 human cases of hepatoma have been chosen with respect to their different seric alpha-fetoprotein (alpha-fp) level and histological characters. cells producing alpha-fp have been studied with specific horseradish-peroxidase-labelled immunoglobulins. ultrastructural examination shows that alpha-fp is present in the cytoplasm of some tumoral hepatocytes. alpha-fp is also present in the cytoplasm of some rare nontumoral hepatocytes of a nonsecreting hepatoma. ultrastructural differences are descri ... | 1978 | 86473 |
| identification of species and capsular types of klebsiella clinical isolates, with special reference to klebsiella planticola. | in the 77 reference strains for klebsiella k types, there are 17 strains (22.1%) of klebsiella planticola, 6 strains (7.8%) of klebsiella oxytoca, 1 strain (1.3%) of klebsiella terrigena, and 53 strains (68.8%) of klebsiella pneumoniae. the species k. planticola, which was originally isolated from botanical and aquatic environments and hence thus named, was also identified at high incidence (81 strains, 18.5%) among the 439 recent clinical isolates of klebsiella species. among these k. planticol ... | 1989 | 2593874 |
| cytoskeleton in the apical region of mouse taste bud cells. | the cytoskeletal structures in the apical region of mouse taste bud cells were examined by immunocytochemistry and electron microscopy. immunostaining for actin showed positive reactions in the apical portions of the taste buds. these regions contained bundles of longitudinally oriented filaments (5-7 nm in diameter) extending from the tip of microvilli to the apical cytoplasm of type i, ii, and iii cells. after incubation with heavy meromyosin, arrowhead formation was observed along these filam ... | 1989 | 2519274 |
| cerebrospinal fluid insulin levels increase during intravenous insulin infusions in man. | we hypothesized that plasma insulin crosses the blood-cerebrospinal fluid (csf) barrier and, as people gain weight, provides a physiological feedback signal to the central nervous system to inhibit food intake and further weight gain. however, it has not been demonstrated in man that insulin can enter the csf from peripheral blood. to test whether increases in plasma insulin result in elevated csf immunoreactive insulin (iri) levels, we infused insulin iv in varying amounts approximating postpra ... | 1987 | 3536982 |
| cerebrospinal fluid insulin levels increase during intravenous insulin infusions in man. | we hypothesized that plasma insulin crosses the blood-cerebrospinal fluid (csf) barrier and, as people gain weight, provides a physiological feedback signal to the central nervous system to inhibit food intake and further weight gain. however, it has not been demonstrated in man that insulin can enter the csf from peripheral blood. to test whether increases in plasma insulin result in elevated csf immunoreactive insulin (iri) levels, we infused insulin iv in varying amounts approximating postpra ... | 1987 | 3536982 |
| assay of all-trans----11-cis-retinoid isomerase activity in bovine retinal pigment epithelium. | | 1990 | 2292965 |
| survival of bacteria during aerosolization. | one form of commercial application of microorganisms, including genetically engineered microorganisms is as an aerosol. to study the effect of aerosol-induced stress on bacterial survival, nonrecombinant spontaneous antibiotic-resistant mutants of four organisms, enterobacter cloacae, erwinia herbicola, klebsiella planticola, and pseudomonas syringae, were sprayed in separate experiments in a greenhouse. samples were collected over a distance of 15 m from the spray site for enumeration. spores o ... | 1990 | 2268156 |
| [treatment of renal cysts by percutaneous drainage and alcoholization. our experience]. | since many years renal cysts have been treated by percutaneous puncture and successive aspiration. the introduction of various sclerosing substances has been associated with this manoeuvre. in our study we examined 44 patients with renal cysts submitted to percutaneous puncture, aspiration and injection with 95% ethanol. we thought right to leave a small pig tail catheter so that the sclerosing substance could be easily and completely eliminated allowing the adhesion of the cystic walls in the f ... | 1990 | 2212715 |
| pyridoxal phosphate-dependent histidine decarboxylases. cloning, sequencing, and expression of genes from klebsiella planticola and enterobacter aerogenes and properties of the overexpressed enzymes. | the hdc genes encoding the inducible pyridoxal-p-dependent histidine decarboxylase (hisdcase) of klebsiella planticola and enterobacter aerogenes were isolated, sequenced, and expressed in escherichia coli under control of the lac promoter, and the overproduced enzymes were purified to homogeneity from the recombinant host. formation of inclusion bodies during synthesis of the e. aerogenes enzyme was avoided by cooling the culture and inducing at 25 degrees c. the cloned enzymes were produced in ... | 1991 | 2033044 |
| the anaerobic utilisation of cyanide in the presence of sugars by microbial cultures can involve an abiotic process. | the degradation of cyanide under anaerobic conditions in the presence of a growing culture of a strain of klebsiella planticola has been shown to be due to a chemical process dependent upon the presence of a reducing sugar in the medium. the conversion of cyanide to ammonia was independent of any biological factors under these conditions. | 1991 | 1884979 |
| [the detection of pathogenic properties in soil nitrogen-fixing enterobacteria]. | two klebsiella planticola nitrogen-binding soil strains have been found capable, when introduced intraperitoneally into white mice, of killing the animals, the ld50 of the strains being 8.8 +/- 0.1 and 9.0 +/- 0.2. in two k. pneumoniae strains isolated from internal organs of sick agricultural animals the presence of nitrogen-binding activity has been established. | 1991 | 1838655 |
| activation of the plasma membrane h(+)-atpase of saccharomyces cerevisiae by addition of hydrogen peroxide. | addition of hydrogen peroxide (greater than 10 mm) to aerated derepressed cells of s. cerevisiae in the absence of substrate caused a boost of endogenous respiration and both intra- and extracellular acidification, without any significant change in cellular atp level. furthermore, a hyperpolarization of the plasma membrane was indicated by an enhanced accumulation of tetraphenylphosphonium in the cells. the extracellular ph attained was as low as 3.5. the acidification could be suspended by the ... | 1991 | 1831983 |
| astrocytes: a possible primary site for colchicine-mediated neurotoxicity in the rat striatum. | ultrastructural changes in rat astrocytes including endfoot swelling and rupture of plasma membranes were seen as early as 1 h after injection of 6.0 micrograms colchicine (co) into the striatum. significant change in neuronal morphology was not seen by 24 h. astrocytes may therefore be primary targets for co-mediated damage, which then may have secondary neurotoxic consequences. | 1991 | 1828783 |
| [diagnosis and therapy of viral hepatitis]. | | 1991 | 1713243 |
| letter: cryptococcus neoformans diagnosed by fine needle aspiration cytology of the lung. | | 1976 | 1065169 |
| bone bonding behavior of mgo-cao-sio2-p2o5-caf2 glass (mother glass of a.w-glass-ceramics). | in this study, it was found that a ca-p layer and a si layer were formed on the interface of the mother glass of apatite-wollastonite containing glass-ceramics (designated aw) and bone tissue. the dissolution of si, ca, and p from glass (mgo-cao-sio2-p2o5-caf2) is necessary to form a chemical film (a si layer and a ca-p layer). the three kinds of glasses used were 1) a mirror surface of the mother glass (mgo 4.6, cao 44.9, sio2 34.2, p2o5 16.3, caf 0.5 weight ratio) of aw (designated g-aw (mirro ... | 1989 | 2738079 |
| adverse effects of the use of unusual phenethylamine compounds sold as illicit amphetamine. | a 40-year-old man, who had regularly taken illicit amphetamine by intra-nasal inhalation for several years without ill effects, was admitted to hospital with signs of massive adrenergic overstimulation shortly after inhaling material which he had purchased in the belief that it was amphetamine. the administration of the beta-blocker practolol produced a paradoxical increase in blood pressure. after his discharge from hospital he suffered disabling feelings of anxiety for several weeks. analysis ... | 1989 | 2770471 |
| results of the flow cytometry actg quality control program: analysis and findings. | the aids clinical trial group's (actg) immunology committee was charged with initiating a quality control program for all laboratories participating in the actg program reporting flow cytometry data. forty-one laboratories were evaluated. this report defines the goals of this program and the subsequent findings after 19 send-outs were made. both hiv positive volunteer donors and normal age-matched donors were used. sample sets included both heparin and edta anticoagulated bloods. laboratories we ... | 1989 | 2785890 |
| the cutworm peridroma saucia (lepidoptera: noctuidae) supports growth and transport of pbr322-bearing bacteria. | variegated cutworms were exposed to bean plants in microcosms sprayed with pbr322-carrying strains of enterobacter cloacae, klebsiella planticola, and erwinia herbicola. the three bacterial species exhibited differential survival on leaves, in soil, and in guts and fecal pellets (frass) of the insects. high numbers of enterobacter cloacae(pbr322) were detected in all samples, while the other species were unable to establish residence in the insect. to assess the impact of this colonization on si ... | 1989 | 2802606 |
| high-performance liquid chromatographic separation and immunological characterization of soluble bovine viral diarrhea virus antigen. | sodium dodecyl sulphate-polyacrylamide gel electrophoresis and western blot analysis of the protein extracts from bovine viral diarrhea virus (bvdv, singer strain) infected primary calf testicle cells (soluble antigen) showed the presence of four virus specific polypeptides of 105, 90, 84 and 67 kilodaltons (kd) the 84-kd being the most abundant. anion-exchange high-performance liquid chromatography (hplc) of soluble antigen separated the virus specific polypeptides in individual peaks while the ... | 1988 | 2832429 |
| some enzymic properties of human lens esterase i. | | 1974 | 4208803 |
| 14c-urea breath analysis, a non-invasive test for campylobacter pylori in the stomach. | | 1987 | 2884468 |
| [isolation of foot-and-mouth disease virus in sheep]. | | 1970 | 4316723 |
| adenyl-cyclase in a transplantable thyroid tumor: loss of ability to respond to tsh. | | 1972 | 4336437 |
| adenyl-cyclase in a transplantable thyroid tumor: loss of ability to respond to tsh. | | 1972 | 4336437 |
| an immunocytochemical analysis of the ontogeny of the microtubule-associated proteins map-2 and tau in the nervous system of the rat. | the developmental distribution patterns of beta-tubulin and the microtubule-associated proteins, map-2 and tau, were studied by immunocytochemistry with monoclonal antibodies. the analysis of the in situ distribution of these proteins in embryonic brain tissue revealed intense immunoreactivity for beta-tubulin in proliferative and migrating neuroblasts. on the contrary, no immunoreactivity for map-2 or tau was detected in this neuroepithelium; specific immunostaining for these maps was only pres ... | 1987 | 3113673 |
| purification and properties of pyridoxal-5'-phosphate-dependent histidine decarboxylases from klebsiella planticola and enterobacter aerogenes. | histidine decarboxylases from klebsiella planticola and enterobacter aerogenes were purified to homogeneity and compared with the histidine decarboxylase from morganella morganii. all three enzymes required pyridoxal 5'-phosphate as a coenzyme, showed optimal activity at ph 6.5, decarboxylated only histidine among the amino acids derived from protein, and were tetramers or dimers of identical subunits. amino-terminal sequences of the three enzymes showed up to 81% homology through residue 33, bu ... | 1987 | 3114230 |
| the distribution of radioiodine-labeled n-isopropyl-p-iodoamphetamine in permanently ischemic brain of the mongolian gerbil. | in a study of the distribution of n-isopropyl-p-131i-iodoamphetamine (imp) in the permanently ischemic brain of 35 mongolian gerbils, the right common carotid artery was ligated under ether anesthesia. after given time intervals, 1.35 mbq (50 microci) of imp was injected into 17 gerbils which had severe neurological symptoms, and into 3 normal gerbils for controls. one minute there after each gerbil was sacrificed and brain autoradiography was performed. the activity of imp in various parts of t ... | 1988 | 3368336 |
| a high-precision selective determination method for free n-acetylneuraminic acid in human serum. | we established a high-precision selective determination method for free n-acetylneuraminic acid in human serum, involving capillary gas chromatography-mass spectrometry with selected ion monitoring and specific separation on a sep pak silica cartridge. in contrast to the value of 800 ng/ml of n-acetylneuraminic acid previously reported by haverkamp et al. (j. haverkamp, r. schauer, and m. wember (1976) hoppe-seyler's z. physiol. chem. 357, 1699), who used the thiobarbituric acid method, the pres ... | 1988 | 3389506 |
| experimental evaluation of the mitroflow pericardial heart valve prosthesis. part ii. pathologic examination. | the results of the morphologic and histopathologic examination of 37 bovine pericardial heart valve prostheses that were retrieved after experimental implantation in chacma baboons for periods of one to twelve months are presented in this study. the implanted prostheses consisted of 33 mitroflow valves, size 21 mm, that belonged to four different groups according to the method of preparation of the pericardium (process i to iv) and 4 commercially available ionescu-shiley valves, size 19 mm. all ... | 1988 | 3421507 |
| determination of thermodynamic parameters for the interaction of a lipid-binding peptide and insulin with a reversed-phase column. | the effect of temperature was investigated on the reversed-phase chromatography of a synthetic lipid associating peptide (lap), with the following sequence (lesflkswlsaleqalka) and on insulin. the lap was chromatographed on a mubondapak-alkylphenyl column with 1% aq. triethylammonium phosphate, ph 3.2-2-propanol (80:20) as the isocratic mobile phase. the insulin was separated on a resolve-c18 column with a mobile phase that contained 0.1 m sodium phosphate, ph 2.0-acetonitrile (71.5:28.5). with ... | 1986 | 3536977 |
| electron microscope study of septum formation in escherichia coli strains b and b-r during synchronous growth. | the formation of cell wall septa was monitored in escherichia coli b and b/r during synchronous growth in glucose media at 37 c by means of electron microscopy. the visible events of septation comprised the following sequence, starting at about 30 min of incubation: (a) bleb formation of the outer membrane; (b) invagination of mucopeptide and cytoplasmic membrane (with associated mesosomes); the outer membrane is excluded from the septum; (c) formation of a cross-wall; (d) ingrowth of the outer ... | 1974 | 4604418 |
| markov-dependency and spectral analyses on spike-counts in mesencephalic reticular neurons during sleep and attentive states. | spontaneous activities of the mesencephalic reticular formation (mrf) neurons of head-restrained cats were recorded to investigate their dynamic properties during sleep and waking. the markov-dependency and spectral analyses were performed on the time series of counts converted from the mrf spike-train. during slow-wave sleep (sws), mrf neurons fired with low markovian properties and had a similar spectral-density curve as white noise; during paradoxical sleep (ps), their firing pattern showed h ... | 1986 | 3697684 |
| renal and non-renal clearances of iothalamate. | an evaluation of the literature indicated that certain aspects of the disposition kinetics of iothalamate, important to the accurate determination of glomerular filtration rate in dogs and humans, remain to be resolved. the simultaneous clearances of iothalamate and inulin in 5 dogs were determined at three steady-state iothalamate plasma levels (2, 10, and 40 micrograms ml-1) following various rates of intravenous infusion. the iothalamate clearances, both renal and non-renal, were concentratio ... | 1986 | 3768490 |
| a new immunization procedure for obtention of anti-leucine-enkephalin antibodies. part ii. effects of olfactory bulb removal on pro-enkephalin related peptides in rat brain. | bilateral olfactory bulb removal (obr) induced both complex behavioral alterations and a decrease of many neurotransmitter levels. we studied brain levels of the pro-enkephalin related peptides 45 days after obr. opioid levels were studied using three different highly specific antisera exhibiting very high affinities in radioimmunoassays in striatum, hypothalamus, hypophysis, brain stem and cortex. methionine enkephalin levels increase significantly in striatum (42%), hypophysis (94%) and hypoth ... | 1986 | 3785586 |
| [hemoglobin g georgia in a turkish family in the netherlands]. | | 1986 | 3754310 |
| nosocomial infection and colonization by klebsiella trevisanii. | during an 18-month period we identified two cases of septicemia and 24 examples of colonization of humans by klebsiella trevisanii. organisms were identified using the api 20ec and api 147 assimilation galleries. of 147 clinical isolates initially identified as k. oxytoca, 18% were found to be k. trevisanii. tracheal aspirate was the most common source of the organism. an extensive environmental sampling survey in the rooms of 12 colonized patients revealed a possible reservoir of the organism o ... | 1986 | 3711281 |
| monitoring of morantel levels in digestive contents by high performance liquid chromatography. | | 1986 | 3806784 |
| the effect of high-energy proton irradiation on the cardiovascular system of the rhesus monkey. | | 1966 | 4957419 |
| "thiocyanate gold": small (2-3 nm) colloidal gold for affinity cytochemical labeling in electron microscopy. | reduction of haucl4 by nascn or kscn produces colloidal gold particles of 2.6 nm in diameter and homogeneous in size (coefficient of variation approximately 15%). the auscn sol forms protein-gold complexes. the amount of protein required to form an auscn-protein complex is best determined in the electron microscope, where serial dilutions of protein with gold sol are inspected for the presence of aggregates. by immuno-electron microscopy scn-gold complexed to protein a is active and visible as i ... | 1985 | 3878348 |
| the production of biliary cirrhosis in macaca mulatta monkeys. | | 1970 | 4982781 |
| protein antigens of encapsulated klebsiella pneumoniae surface exposed after growth in the presence of subinhibitory concentrations of cephalosporins. | it recently has been reported by us that cephalosporins, at a concentration below that influencing growth rate, reduced the production of enterochelin and capsule formation of iron-depleted klebsiella pneumoniae. we now report on the antigenicity of the outer membrane components and surface-exposed protein antigens of iron-depleted cells grown in the presence or absence of cephalosporins. all major outer membrane proteins, including iron-regulated membrane proteins, were immunogenic. encapsulate ... | 1985 | 3914857 |
| [effect of vagotomy combined with gastric resection on morphofunctional shifts in the peripheral autonomic nervous system]. | | 1985 | 4068556 |
| local cerebral glucose utilization in normal female rats: variations during the estrous cycle and comparison with males. | the quantitative 2-[14c]deoxyglucose autoradiographic method was used to study the fluctuations of energy metabolism in discrete brain regions of female rats during the estrous cycle. a consistent though statistically nonsignificant cyclic variation in average glucose utilization of the brain as a whole was observed. highest levels of glucose utilization occurred during proestrus and metestrus, whereas lower rates were found during estrus and diestrus. statistically significant fluctuations were ... | 1985 | 4040921 |
| a comparative examination of the in vitro metabolism of five cyclopenta[a]phenanthrenes of varying carcinogenic potential. | metabolites of 15,16-dihydrocyclopenta[a]phenanthren-17-one and its 1- and 12-methyl homologues (all non-carcinogens) along with those from the 11-methyl and 11,12-dimethyl-17-ketones (carcinogens), produced in vitro by hepatic microsomes from methylcholanthrene induced rats, were separated by reverse phase h.p.l.c. identifications of individual metabolites were based upon elution times, u.v. spectra, and in some cases by mass spectrometry, circular dichroism, and identity with synthetic derivat ... | 1985 | 4017190 |
| early events in cell-animal virus interactions. | | 1973 | 4200163 |
| [hepatitis and the australia antigen]. | | 1970 | 4249567 |
| a comparative examination of the in vitro metabolism of five cyclopenta[a]phenanthrenes of varying carcinogenic potential. | metabolites of 15,16-dihydrocyclopenta[a]phenanthren-17-one and its 1- and 12-methyl homologues (all non-carcinogens) along with those from the 11-methyl and 11,12-dimethyl-17-ketones (carcinogens), produced in vitro by hepatic microsomes from methylcholanthrene induced rats, were separated by reverse phase h.p.l.c. identifications of individual metabolites were based upon elution times, u.v. spectra, and in some cases by mass spectrometry, circular dichroism, and identity with synthetic derivat ... | 1985 | 4017190 |
| [vasomotor properties of mercaptoethylguanidine (meg) in the rat]. | | 1965 | 4161764 |
| cultures of tissues from patients with amyotrophic lateral sclerosis. | | 1973 | 4126530 |
| outbreaks of monkeypox and serological surveys in nonhuman primates. | in connexion with the recent detection of cases of monkeypox in man in west and central africa, the frequency of monkeypox outbreaks in monkeys since 1958, when the disease was first recognized in captive animals, has been investigated. special incidence surveys were made for this purpose. during the last 3 years, a serological survey has been conducted to find natural foci of monkeypox virus, and a total of 2 242 sera from monkeys of different species from various parts of africa and asia have ... | 1972 | 4340222 |
| septic arthritis of the hip joint secondary to umbilical artery catheterization associated with transient femoral and sciatic neuropathy. | | 1974 | 4365200 |
| evidence for a role of the epstein-barr virus in the etiology of human lymphoma. | | 1974 | 4368706 |
| determination of thermodynamic parameters for the interaction of a lipid-binding peptide and insulin with a reversed-phase column. | the effect of temperature was investigated on the reversed-phase chromatography of a synthetic lipid associating peptide (lap), with the following sequence (lesflkswlsaleqalka) and on insulin. the lap was chromatographed on a mubondapak-alkylphenyl column with 1% aq. triethylammonium phosphate, ph 3.2-2-propanol (80:20) as the isocratic mobile phase. the insulin was separated on a resolve-c18 column with a mobile phase that contained 0.1 m sodium phosphate, ph 2.0-acetonitrile (71.5:28.5). with ... | 1986 | 3536977 |
| response of rat thyroid oxidative enzymes to tsh. | | 1966 | 4379920 |
| physiological effect of orally administered endotoxin to man. | | 1974 | 4616057 |
| [balantidium coli dysentery syndrome in children]. | | 1974 | 4473196 |
| altered heat-shock response in polyamine-depleted bacteria. | a polyamine-auxotrophic mutant of e. coli was cultivated in the presence or absence of putrescine and submitted to heat shock over 3 different ranges of temperature. in all cases, protein synthetic capacity measured in comparison to that of cultures at the preshift temperature was much higher in polyamine-depleted bacteria under thermic stress. addition of putrescine only before the shift-up was able to restore gradually normal control of the relative protein synthetic capacity. | 1986 | 3516724 |
| phylogenetic analyses of klebsiella species delineate klebsiella and raoultella gen. nov., with description of raoultella ornithinolytica comb. nov., raoultella terrigena comb. nov. and raoultella planticola comb. nov. | the phylogenetic relationships of the type strains of 9 klebsiella species and 20 species from 11 genera of the family enterobacteriaceae were investigated by performing a comparative analysis of the sequences of the 16s rrna and rpob genes. the sequence data were phylogenetically analysed by the neighbourjoining and parsimony methods. the phylogenetic inference of the sequence comparison confirmed that the genus klebsiella is heterogeneous and composed of species which form three clusters that ... | 2001 | 11411716 |
| giardia intestinalis. a prospective investigation of the prevalence in denmark. | | 1973 | 4697076 |
| arterial lesions due to rejection in human kidney allografts. | | 1967 | 4862526 |
| effect of previous injection of homologous embryonic tissue on the growth of certain transplantable mouse tumours. | | 1967 | 4862516 |
| the maximum derivatives of left ventricular pressure and transverse internal diameter as indices of the inotropic state of the left ventricle in conscious dogs. | 1. in normal, conscious dogs, i.v. infusion of isoprenaline caused inases in heart rate and the maximal derivatives of left ventricular pressure (dp/dt max) and left ventricular internal diameter (dd/dt max). the changes in both derivatives were linearly related to the increase in heart rate.2. increments in heart rate produced by right atrial pacing caused only minimally significant increases in both dp/dt max and dd/dt max at heart rates of 180 beats/min. increases in heart rate, with end dias ... | 1973 | 4772400 |
| some aspects of salt and water balance of feral goats from arid islands. | | 1974 | 4817419 |
| chromatography and activity of thiol-subtilisin. | | 1969 | 4975909 |
| method for differentiating klebsiella planticola and klebsiella terrigena from other klebsiella species. | conventional methods usually fail to identify klebsiella planticola and klebsiella terrigena, which represent up to 19% of clinical klebsiella isolates. by combining four carbon substrate assimilation tests and two conventional tests, the method identified these species with a specificity and a sensitivity of 100%. overall, sensitivity for klebsiella identification was 94.7%. | 1994 | 8027329 |
| a histidine decarboxylase gene encoded by the vibrio anguillarum plasmid pjm1 is essential for virulence: histamine is a precursor in the biosynthesis of anguibactin. | we have identified and sequenced an hdc gene in the vibrio anguillarum plasmid pjm1 which encodes a histidine decarboxylase enzyme and is an essential component for the biosynthesis of anguibactin. the open reading frame corresponds to a protein of 386 amino acids with a calculated molecular mass of 44,259.69 da. the amino acid sequence has extensive homology with the pyridoxal-p-dependent histidine decarboxylases of morganella morganii, klebsiella planticola, and enterobacter aerogenes. tn3-hoh ... | 1995 | 7752899 |
| formate and ethanol are the major products of glycerol fermentation produced by a klebsiella planticola strain isolated from red deer. | the rumen contents of red deer (cervus elaphus) were used to isolate bacterial capable of fermenting glycerol. the biochemistry, physiology, morphology and phylogeny of one isolate were studied in detail. the isolate (dr3) was tentatively identified as a strain of the species klebsiella planticola as based on phenotypic characterization. the data obtained from 16s rrna sequence analysis showed that the deer rumen isolate dr3 was 99.7% similar to the type strain of kl. planticola (dsm 3069t), thu ... | 1997 | 9281820 |
| kinetic analysis and comparison of models of xylose metabolism by klebsiella planticola. | a model for the degradation of xylose and ethanol production by klebsiella planticola is proposed and compared with the exponential and michaelis-menten approaches. this model is based on the energy system diagrams and it is a simplified version of a previous model developed for the glucose and ethanol kinetics of the yeast saccharomices cerevisiae. in this model the dynamics of the substrate and of the final product are strictly related by means of the cellular activity. this model shows superi ... | 1996 | 8858100 |
| identification of clinical isolates of indole-positive klebsiella spp., including klebsiella planticola, and a genetic and molecular analysis of their beta-lactamases. | in a collection of 43 indole-positive klebsiella clinical isolates, which were initially identified as klebsiella oxytoca, there were 18 isolates which exhibited a pattern characteristic of extended-spectrum beta-lactamase (esbl) resistance. this study aimed to confirm their identity by biochemical tests and by pcr and to determine the genetic basis for their resistance to the beta-lactams and broad-spectrum cephalosporins. chromosomal beta-lactamase genes were analyzed by pcr, and plasmid-media ... | 1997 | 9276417 |
| klebsiella trevisanii colonisation and septicaemia. | | 1984 | 6143213 |
| effect of aerosolization on culturability and viability of gram-negative bacteria. | estimations of the bacterial content of air can be more easily made now than a decade ago, with colony formation the method of choice for enumeration of airborne bacteria. however, plate counts are subject to error because bacteria exposed to the air may remain viable yet lose the ability to form colonies, i.e., they become viable but nonculturable. if airborne bacteria exhibit this phenomenon, colony formation data will significantly underestimate the bacterial populations in air samples. the o ... | 1997 | 9293010 |
| isolation of klebsiella planticola from newborns in a neonatal ward. | this is the first report on the isolation of klebsiella planticola from neonates. over a period of 1 year, oropharyngeal and rectal swab specimens from 131 newborns on a neonatal ward were monitored for klebsiella spp. thirteen strains of k. planticola could be isolated, and these represented 9% of all klebsiella spp. found. capsule type k27 predominated, followed by capsule type k68. our findings suggest a significant rate of colonization by k. planticola among neonates. | 1998 | 9666015 |
| the influence of bacterial inoculants on the microbial ecology of aerobic spoilage of barley silage. | the aerobic decomposition of barley silage treated with two inoculants (laca and lacb) containing mixtures of lactobacillus plantarum and enterococcus faecium was investigated over a 28-day period. initially, yeast and bacterial populations were larger in silage inoculated with laca than in silage treated with lacb or water alone (control). differences in the succession of yeasts in silage treated with laca were observed relative to the other two treatments. from silage treatment with laca, issa ... | 1999 | 10349723 |
| phylogenetic diversity of klebsiella pneumoniae and klebsiella oxytoca clinical isolates revealed by randomly amplified polymorphic dna, gyra and parc genes sequencing and automated ribotyping. | the infra-specific phylogenetic diversity and genetic structure of both klebsiella pneumoniae and klebsiella oxytoca was investigated using a combination of randomly amplified polymorphic dna (rapd) analysis, sequencing of gyra and parc genes, and automated ribotyping. after rapd analysis with four independent primers of 120 clinical isolates collected from 22 european hospitals in 13 countries, k. pneumoniae isolates fell into three clusters and k. oxytoca isolates fell into two clusters, while ... | 2001 | 11411715 |
| heterogeneity of antisera to human growth hormone, demonstrated by the immunofluorescence technique. | | 1968 | 4880317 |
| expression of putative virulence factors by clinical isolates of klebsiella planticola. | a total of 92 clinical isolates of klebsiella planticola from man was examined with respect to the production of haemagglutinins and siderophores, serum resistance and distribution of capsular types. for comparison, a group of 207 clinical isolates of k. pneumoniae was also studied. the percentages of k. planticola strains able to express mannose-sensitive haemagglutination, indicating type 1 fimbriae (83%) and mannose-resistant and klebsiella-like agglutination, indicating type 3 fimbriae (69%) ... | 2000 | 10670561 |
| [antro-duodeno-pancreatic functional relation]. | | 1971 | 4944407 |
| further studies on rpos in enterobacteria: identification of rpos in enterobacter cloacae and kluyvera cryocrescens. | rpos, the alternative sigma factor sigma(s), is important for bacterial survival under extreme conditions. many enterobacteria are opportunistic human pathogens and their ability to survive in a changing environment could be an essential step for their virulence. to determine the presence of this gene in enteric bacteria, an escherichia coli rpos probe was constructed and used to detect the presence of this gene in different species. a gene homologous to rpos was found in citrobacter amalonaticu ... | 2001 | 11491080 |
| 4-hydroxybenzoate uptake in klebsiella planticola strain dsz1 is driven by deltaph. | klebsiella planticola strain dsz1 has the ability to degrade different aromatic compounds such as benzoate and organochlorinated as propachlor and alachlor. dsz1 strain cells mineralised 4-hydroxybenzoate (4hba) through a meta-cleavage pathway, yielding protocatechuate as dihydroxylated intermediate, with a specific rate of co2 formation 0.12 x 10-6 (cpm/od) h-1, and a rate of 4-hba utilisation of 0.75 mmol h-1. aerobically the 4hba transport system is driven by gradient of protons (deltaph), bu ... | 2002 | 11727038 |
| klebsiella pneumoniae produces no histamine: raoultella planticola and raoultella ornithinolytica strains are histamine producers. | histamine fish poisoning is caused by histamine-producing bacteria (hpb). klebsiella pneumoniae and klebsiella oxytoca are the best-known hpb in fish. however, 22 strains of hpb from fish first identified as k. pneumoniae or k. oxytoca by commercialized systems were later correctly identified as raoultella planticola (formerly klebsiella planticola) by additional tests. similarly, five strains of raoultella ornithinolytica (formerly klebsiella ornithinolytica) were isolated from fish as new hpb. ... | 2002 | 12089029 |
| the renal medulla as an antihypertensive organ. | | 1970 | 4917704 |
| antimicrobial resistance in gram-negative bacilli isolated from infant formulas. | a total of 90 samples of infant formula (if) were collected from the lactary of a teaching hospital, during a 4-month period from july to august 1999. the sanitary conditions of the formulas were analyzed, and a physiological characterization of gram-negative bacillus isolates and antimicrobial susceptibility testing were performed. colony counts were considered to be unacceptable for the majority of the if samples and the contamination rates were related to inadequate handling. coliforms (35 de ... | 2003 | 14638421 |
| dna based classification of food associated enterobacteriaceae previously identified by biolog gn microplates. | enterobacteriaceae are frequently isolated from food products and it is essential to have methods for correct identification for both food hygiene and epidemiology reasons. phenotypic methods are not always sufficient and have to be supplemented by dna based methods. in the present study, 70 strains of enterobacteriaceae derived from milk, fish and meat that had previously been identified by biolog gn microplates were genomically classified together with 15 representative type strains of species ... | 2004 | 15046311 |
| high-level expression of a recombinant protein in klebsiella planticola owing to induced secretion into the culture medium. | the tn5-based transposon tn5-kil3 (miksch et al. 1997c) bearing the kil gene of the cole1 plasmid of escherichia coli, which mediates controlled export of periplasmic proteins into the culture medium, was stably integrated into the chromosome of klebsiella planticola with high transposition frequency. a bacillus hybrid beta-glucanase located on an rsf1010-derived plasmid was mobilized from e. coli to k. planticola and used as a reporter protein to select strains with high expression and secretio ... | 1999 | 10390821 |
| recommended test panel for differentiation of klebsiella species on the basis of a trilateral interlaboratory evaluation of 18 biochemical tests. | klebsiella pneumoniae and klebsiella oxytoca are the two most frequently encountered klebsiella species giving rise to infections in humans, but other klebsiella species can also be found in clinical specimens: klebsiella ozaenae, klebsiella rhinoscleromatis, klebsiella terrigena, klebsiella planticola, klebsiella ornithinolytica, and enterobacter aerogenes (klebsiella mobilis). however, many of these species are indistinguishable by the conventional methods employed routinely in the clinical mi ... | 2004 | 15297514 |