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plasmid borne virulence of enterobacter hafniae.the strain classified as enterobacter hafniae has been isolated in a severe epidemy of porcine diarrhoea. its pathogenicity has been found to be cotransmissible with resistance to oxytetracycline when the strain was grown in a mixed culture with some nonpathogenic e. hafniae strains as well as with escherichia coli. toxinogenity seems to be responsible for virulence of the strain.197876419
purification and assay of murine leukemia viruses. 197985237
similarity of casein- and endotoxin-induced, myeloma- associated and aged sjl/j amyloid in various strains of mice.amyloidosis was induced in a number of strains of mice by repeated injections of casein and endotoxin. spontaneous amyloid was obtained from balb/c mice bearing a myeloma tumor (igg2a producing mopc 173 tumor) and from aged sjl/j mice. both the induced and spontaneous forms were similar in their size, immunological reactivity, peptide maps and in the susceptibility of histological sections to oxidizing agents with or without trypsin digestion. since case-induced murine amyloid resembles the noni ...197987374
moloney virus-induced cell surface antigens and histocompatibility antigens are located on distinct molecules. 197987439
passive transfer in diabetes mellitus. 197988629
modulation of cellular interactions between c3h/10t1/2 cells and their transformed counterparts by phosphodiesterase inhibitors.it has been demonstrated previously that nontransformed c3h/10t1/2cl8 mouse embryo fibroblasts (10t1/2) can induce a state of reversible growth inhibition in cocultured malignantly transformed mouse fibroblasts and that this inhibition is modulated by serum concentration. the present study suggests that cyclic nucleotides may be implicated in this intercellular communication. the phosphodiesterase inhibitors theophylline, caffeine, and 3-isobutyl-1-methylxanthine (ibx) at concentrations of 10(-3 ...197989899
alpha-fetoprotein as a carrier protein in plasma and its bilirubin-binding ability.the bilirubin-binding ability of human alpha-fetoproteins, which were purified from fetal cord serum and from ascites fluid of a hepatoma-bearing patient, was examined by the difference spectrum and the jacobsen peroxidase methods. the difference spectrum observed as a result of the specific binding of bilirubin to alpha-fetoprotein had a maximum at 482 nm, and this pattern was quite similar to that observed for serum albumin. the result obtained by the difference spectrum method showed that 1 m ...197989900
the indirect rat mast cell degranulation test reconsidered.the indirect rat mast cell degranulation test for the detection of human reaginic antibodies was reinvestigated. when the mast cells were incubated with human reaginic serum at concentrations exceeding approximately 3%, a massive, non-specific histamine release occurred, irrespective of the presence or absence of allergen. at tolerated serum concentrations (below 3%), the allergen concentration did not influence the release process. we conclude that the indirect rat mast cell test is not applica ...197990655
connectivity of the auditory forebrain nuclei in the guinea fowl (numida meleagris).injection of tritiated leucine and proline into the nucleus ovoidalis of the guinea fowl (numida meleagris) produces terminal labeling in the palaeostriatum and in three adjacent zones (field l1-l3) of the auditory neostriatum (an). l2, situated between l1 and l3, receives the main input and corresponds to the former field l of rose. these neuroanatomically defined zones of the auditory neostriatum are also characterized by differing properties of their neurons. injection of radioactive material ...197991442
occurrence of beta 2-microglobulin in mammalian lymphocytes and erythrocytes.cell-associated beta 2-microglobulin (beta 2m) has been studied by quantitative techniques in lymphocytes and erythrocytes of guinea pig, rabbit, rat and man. beta 2m was solubilized by sonication followed by treatment with acid, deoxycholate or thiocyanate, and then determined by radioimmunoassay. the average number of beta 2m molecules per lymphocyte, estimated after acidification, varied between 0.89 x 10(5) and 7.1 x 10(5). rat erythrocytes contained 3.0 x 10(3) molecules per cell, whereas n ...197991519
morphochemic studies on histamine and monoaminooxidase in the liver and studies on histaminolytic activity of guinea pig plasma in the course of sensitization with heterogenic protein. 197991564
antigen specific t cell-mediated suppression. vi. properties of in vitro generated l-glutamic acid60-l-alanine30-l-tyrosine10 (gat)-specific t-suppressor factor(s) (gat-tsf) in responder mouse strains. 197991643
olivocerebellar projections in the cat studied by means of anterograde axonal transport of labelled amino acids as tracers. 197992769
selective aromatic-hydrophobic binding and fractionation of immunoglobulin by means of phenyl-(ch2)n-nh-substituted agaroses. 197992988
a further member of the yucaipa group isolated from the common wren (troglodytes troglodytes).in 1977, 477 small birds of 52 species were virologically examined. five cloacal swabs from troglodytes troglodytes yielded two paramyxovirus strains which were found antigenically related to, but not identical with ch/yucaipa/california/56 virus according to three serological tests. the ecological significance of these findings and the possibility of paramyxovirus classification into four types are discussed.197994770
[immunological problems in oncology (author's transl)].the authors, after a view of the problems of immunology, regarding the most recent discoveries, deal with the conclusions about the immunological reactions in research and clinical oncology. many specific antibodies, formed as a result of the tumor, have been discovered in the field of research. it is impossible to say the same about human tumors and the probable hypothesis are numerous. the results until now obtained, even if they don't give a definite response, show that the immune defense of ...197995475
[observation on the antigenic affinity of leptospira and brucella in the serological examination of cattle].36,9% bovine serums which showed a non-specific reaction in examination for brucellosis had titre greater than or equal to 1:400 leptospirosis agglutinins. 15.1% of the serums showed a positive reaction (greater than 1:800), mostly those against l. sejroe and l. grippotyphosa. the cross reactions, found in individual cases within up to 39 days since the first reaction to brucellosis in cattle may signalize early leptospira infections.197896568
the physiological disposition of the anti-tussive agent, 1,2,3,4a,9b-hexahydro-8,9b-dimethyl-4-[3-(4-methylpiperazin-1-yl)propionamido]dibenzofuran-3-one dihydrochloride azipranone, in man, rat, dog and baboon.1. the absorption, tissue distrigution, elimination and biotransformation of the anti-tussive agent azipranone labelled with 14c have been investigated after oral dosing to rat, dog, baboon and man and parenteral administration to rat and baboon. 2. levels of radioactivity in plasma were maximal within 20 min of dosing in the rat and after 1-2 h in the remaining species. the concn. declined thereafter with a half-life estimated at 1, 3-4 and 18-24 h for rat, dog, and baboon and man respectively. ...197896617
[functional study of the rat olfactory bulb by a 14c-2-deoxyglucose autoradiographic technic].the autoradiographic method has been used in the rat to map active regions in the olfactory bulb after a pulse of 14c-2-deoxyglucose with electrical stimulation of the lateral olfactory tract. the highest optical densities were found at the external plexiform, mitral, internal plexiform and granular layers; the lowest was found in the glomerular layer.197896988
behavioral measurement measurements of excitability changes in reward sites in the dog: a frequency dependent effect. 197899757
[clinical studies of childhood empyema experienced for the past 12 years (author's transl)]. 1978101626
effect of hypotension induced by sodium nitrocyanoferrate (iii) on the release of arginine-vasopressin in the unanaesthetized monkey. 1978101639
a receptor for polymerized human and chimpanzee albumins on hepatitis b virus particles co-occurring with hbeag.a receptor for polymerized human serum albumin was demonstrated on dane particles as well as on 20-nm hepatitis b surface antigen particles, isolated from asymptomatic carriers of hepatitis b virus who were positive for hbeag. in contrast, such receptor was not born by 20-nm hepatitis b surface antigen particles obtained from carriers positive for antibody to hbeag. hepatitis b surface antigen particles with the receptor were heavier than those without, and when treated with pronase, they became ...1979103774
transcription and translation in a pleiotropic streptomycin-resistant mutant of escherichia coli.the role of the ribosomal protein s12 (streptomycin protein) in ribosome function and in other metabolic processes in the cell has been investigated. a spontaneous streptomycin-resistant strain of escherichia coli (sm3) carrying a mutation in the rpsl gene is deficient in its ability to induce the synthesis of the enzyme bets-galactosidase. it was demonstrated that the reduced rate of enzyme synthesis results from deficiencies in both the transcription of the lactose operon and translation of th ...1979104958
demonstration of the intermediate cells of billingham and medawar in the epidermis of macaca mulatta and rhesus monkey.the digest of the pure epidermis preparation from macaca mulatta demonstrated three types of dendritic cells i.e. (1) dopa-positive melanocytes, (2) dopa-negative langerhans' cells and (3) weakly dopa-positive intermediate cells of billingham and medawar. the last type of cell was found to have characteristics being intermediate between those of the melanocytes and of the langerhans' cell. a similar digest prepared from the epidermis of the rhesus monkey demonstrated isomorphous dendritic cells ...1979106619
renal emphysema of the tranplanted kidney: sonographic appearance. 1979106703
circadian control of thermoregulation in the squirrel monkey, saimiri sciureus.the characteristics and control of the circadian rhythms of core body temperature (colonic) and skin temperature (tail) were studied in chair-acclimatized squirrel monkeys (saimiri sciureus). when animals were entrained to a light-dark cycle (12 h 600 lx; 12 h less than 1 lx) these two temperatures displayed prominent, reproducible, tightly coupled circadian rhythms. in contsant light of 600 lx, where no other effective circadian time cues were present, both temperature rhythms persisted with fr ...1979106737
reproductive capacity of rhesus monkeys following bilateral ovarian x-irradiation.the effect of ovarian x-irradiation of the rhesus monkey upon menstrual cycle length, duration of menstrual bleeding, excretion of gonadotrophin in the urine, concentration of gonadotrophin in the pituitary gland, ovarian histology, and breeding performance was investigated. doses below 4000 r had no significant effect upon the above criteria. by contrast, doses of 4000 to 7000 r rapidly induced amenorrhoea in most animals, but unexpectedly these animals did not excrete greater than normal amoun ...1979108398
inhibitory effect of normal mammalian sera on the rosette formation of human lymphocytes with sheep red blood cells. 1975123840
characterization of 5.8s ribosomal ribonucleic acid in neurospora crassa.neurospora crassa ribosomes contain a species of ribonucleic acid (rna) of molecular weight 54,000, similar to 5.8s ribosomal rna previously described for other eukaryotic organisms. the 5.8s rna from n. crassa was found to be released by heat treatment at 60 c from 25s ribosomal rna but not from 18s ribosomal rna. the base composition of n. crassa 5.8s rna was similar to that of 5.8s rna from saccharomyces cerevisiae, but differed from animal 5.8s rna. during the course of this study, it was di ...1975126993
[economic effectiveness of veterinary measures in swine balantidiasis]. 1976130713
treponemal antigen in immunopathogenesis of syphilitic glomerulonephritis.a patient with syphilitic glomerulonephritis had a renal biopsy and was treated for secondary syphilis. light, electron, and immunofluorescence microscopic studies revealed an acute proliferative glomerulonephritis with subepithelial, intramembranous, and subendothelial immune complex deposits containing igg, iga, igm, c4, and c3. similar local deposits containing predominantly igm were noted in areas of mesangial proliferation. indirect fluorescent antibody studies employing rabbit treponemal a ...1976130804
repressible and inducible enzymic forms of dimethylnitrosamine-demethylase. 1977143144
occupational allergic contact dermatitis to virginiamycin, an antibiotic used as a food additive for pigs and poultry.a warehouseman aged 31 working in a pharmaceutical factory got an occupational contact dermatitis to virginiamycin (factor m), an antibiotic used as a food additive for pigs and poultry. a review is made of contact dermatitis to virginiamycin and to pristinamycin.1978149638
ultrastructural observations on fudr-induced cell death and subsequent elimination of cell debris.twelve-day mouse embryos were treated with fluorodeoxyuridine (fudr) and sacrificed at various time intervals after treatment. the neuroepithelial cells were then examined to determine by electron microscopy the primary site of action of the drug. about two hours after treatment mitotic activity ceased and a number of cells were found with a normal interphase nucleus, but with a cytoplasm in which the ribosomes had lost their normal polysomal configuration and were dispersed as single ribosomes. ...1978150063
irs, hew threaten triple-a bonds. 1978152403
[separation and characterization of subcellular organoids by tissue fractionation]. 1975152443
proton translocation in cytochrome-deficient mutants of escherichia coli.cytochrome-deficient cells of a strain of escherichia coli lacking 5-amino-levulinate synthetase have been used to study proton translocation associated with the reduced nicotinamide adenine dinucleotide (nadh) dehydrogenase region of the electron transport chain. menadione was used as electron acceptor, and mannitol was used as the substrate for the generation of intracellular nadh. the effects of iron deficiency on nadh- and d-lactate-menadione reductase activities were studied in iron-deficie ...1979154508
in-vitro activity of methronidazole on the cysts of entamoeba histolytica. 1975166039
tissue specific differences in the 2'-o-methylation of eukaryotic 5.8s ribosomal rna. 1975178541
effectiveness of orally administered bordetella pertussis vaccine in mice.oral administration of killed bordetella pertussis organisms to mice results in increased resistance to an intracerebral infection with virulent b. pertussis cells. the rate of survival is dependent on the dose of antigen. but besides specific systemic immunity, which is persistent over a long period, also transient non-specific resistance is increased. these effects are evidently induced without penetration of bacterial substances into the circulation.1976182602
properties of a baboon lymphotropic herpesvirus related to epstein-barr virus.three lymphoblastoid cell lines were established from splenic lymphocytes of a lymphomatous baboon (papio hamadryas) by co-cultivation of the lymphocytes with x-irradiated cells of marmoset or baboon lymphoblastoid cell cultures; the baboon splenic lymphocytes failed to grow when cultured alone. a herpesvirus, associated with each cell line, was identified by immunofluorescence, molecular hybridization and electron microscopy. antigenic comparison with epstein-barr virus (ebv) showed that the ba ...1976186420
rat adrenocortical dynamics.1. the dynamics of the adrenocortical response to adrenocorticotrophic hormone (acth) was studied in anaesthetized, acutely hypophysectomized male rats. 2. acth test-signals were applied either in a jugular vein ('intact infused preparation') or in the aorta through the coeliac artery with the aorta ligated below this artery ('isolated in situ perfused preparation'). the adrenocortical responses were measured directly in samples serially taken from the left adrenal vein. 3. tested acth signals w ...1977191596
[effect of somatotropic hormone on certain indices related to carbohydrate and fat metabolism in sheep].investigated was the effect of the somatotropic hormone (sth) at doses of 0.3 mg/kg and 1 mg/kg liveweight on the amount of insulin, glucose, and free fatty acids (ffa) in the serum up to the 12th hour following treatment. the activity of glucose-6-phosphatase and the amount of glycogen were studied in tissue samples taken through biopsy of the liver at the 2nd, 4th, 6th, and 8th hour after treatment with sth. the content of blood sugar decreased, and that of insulin increased in the course of o ...1976193239
splenic influence of t cell function: the immunobiology of the inbred hereditarily asplenic mouse. 1977194980
the immunological approach to study of possibly virus-induced human malignancies using the epstein-barr virus as example. 1978205906
biologic properties of lyophilized live typhoid vaccine strains. 1979262514
interaction of blood with enterobacteriaceae: hemolysis, hemagglutination, fibrinolysis.one thousand eighty enterobacteriaceae, isolated during a period of three months, were tested for the presence of hemolysis, hemagglutination and fibrinolysis on human erythrocytes and plasma. the fibrinolysis on human erythrocytes and plasma. the escherichia coli have the greatest diversity of action on the blood. of 481 strains, 32.9% were hemolytic, 21.9% fibrinolytic, and 10.5% hemagglutinating. of 190 strains of klebsiella, 95.2% were fibrinolytic. of 53 strains of enterobacter, 90.6% were ...1977320861
the identification of new rna-binding proteins in the escherichia coli ribosome. 1977321250
therapy for experimental intraabdominal sepsis: comparison of four cephalosporins with clindamycin plus gentamicin.an experimental model of intraabdominal sepsis in rats was used for evaluation of treatment with four cephalosporins: cephalothin, cefazolin, cefoxitin, and cefamandole. doses were 115 mg of each of the cephalosporins/kg and, in additional experiments, 350 mg of cephalothin or cefazolin/kg. response to treatment was evaluated by mortality rate and the incidence of intraabdominal abscess. all four cephalosporins and gentamicin produced a similar reduction in mortality rates. there was a marked va ...1977321707
[enterobacteria erroneously identified as salmonella in human faeces (author's transl)].to differentiate citrobacter freundii, enterobacter hafniae, proteus mirabilis and alteromonas putrefaciens from salmonella strains isolated in faeces, the author recommends the use of "hajna-kligler" medium, "nitrate-motility-mannitol" medium, "urease-indole-trytophane desaminase" medium and glycerol peptonated water. the lysis of salmonella and e. hafniae strains by specific phages (felix and callow o: 1 salmonella phage; guinee and valkenburg hafnia phage) is emphasized.1977335345
differentiation of serratia marcescens and serratia liquefaciens by tests for lipase and phospholipase production.the production of lipase and phospolipase by certain members of the enterobacteriaceae was examined by thin-layer chromatography of resting-cell suspensions incubated with triolein or lecithin. most strains of serratia marcescens produced both enzymes while most strains of serratia liquefaciens exhibited strong lipase but only a minor phospholipase activity. enterobacter spp. (25 strains), klebsiella pneumoniae (20 strains), escherichia coli (15 strains), citrobacter freundii (7 strains) and pro ...1978355631
replacement of pseudouridine in transfer rna by 5-fluorouridine does not affect the ability to stimulate the synthesis of guanosine 5'-triphosphate 3'-diphosphate.the requirement for ribothymidine and pseudouridine in the tpsicg loop of trna for its activity in the ribosome and trna-stimulated synthesis of guanosine 5'-triphosphate 3'-diphosphate (pppgpp) by stringent factor has been tested by the use of a purified trnaphe (883 pmol of phenylalanine incorporated/a260 unit) in which 92% of the pseudouridine, 98% of the ribothymidine, 98% of the dihydrouridine, and 88% of the uridines were substituted by 5-fluorouridine. this trna was quantitatively as acti ...1978356873
guinea pig lung lavage cells after intranasal bcg sensitization.recent studies have suggested that intranasal administration of antigen can induce local cell-mediated immunity in lung lavage cells. the present study was designed to examine the changes in composition of lung lavage cells and their capacity to produce the lymphokine migration inhibitory factor after intranasal immunization with bcg in guinea pigs. results indicate that guinea pigs responded to respiratory tract bcg infection with an increase in immunocompetent cells in the bronchoalveolar trac ...1979387595
differentiation of selected enterobacteriaceae by pyrolysis-gas-liquid chromatography.pyrolysis-gas-liquid chromatography was used to differentiate selected species of enterobacteriaceae. individual cultures of salmonella typhi, hafnia alvei, and proteus vulgaris, and 12 strains of yersinia enterocolitica were grown in nutrient broth. after harvest and lyophilization, the bacterial samples were pyrolyzed at 900 degrees c, and their volatile fractions were separated on a 50-m capillary column coated with carbowax 20m. the resulting pyrolysis elution patterns (pyrograms) of the fou ...1979393169
an xyy man.this case report describes a young man with a 47, xyy karyotype who was convicted of arson. he suffered from a cardiac disorder which may well have been part of the xyy syndrome rather than a chance association. his abnormal karyotype was disclosed in court and used by the defence in a plea in mitigation.1979497632
spinal analgesia and sedation of goats with lignocaine and xylazine.spinal anaesthesia of 20 does undergoing laparotomies was accomplished with epidural injections of 2 per cent lignocaine at a dosage of 1 ml per 4.55 kg in 16 goats. epidural injection resulted in paralysis lasting 198.5 minutes +/- 36.6 minutes. subarachnlid injection resulted in paralysis lasting 180.8 minutes +/- 44.9 minutes. xylazine was given intramuscularly at the dosage of 0.11 mg per kg after posterior paralysis was complete. these treatments resulted in effective analgesia for surgical ...1979516312
[diagnostic value of the acid production from galacturonate to differentiate strains of "salmonella" sub-genus i and monophasic sub-genus iii from the other "salmonella" strains belonging to sub-genera ii, diphasic iii, iv, "citrobacter" and "hafnia alvei" (author's transl)].two methods were compared: (1) the ability to use sodium glacturonate as source of carbon and energy in defined medium; (2) the ability to produce acid by fermentation of this substrate in peptone water with phenol red. results of growth on, acid formation from, galacturonate are identical. salmonella strains of sub-genus i and monophasic serotypes of sub-genus iii do not use galacturonate. salmonella of sub-genera ii and iv as well as diphasic serotypes of sub-genus iii use galacturonate. glact ...1979533071
pharmacology and efficacy of netilmicin.twenty-six patients, 20 to 77 years of age, were treated with netilmicin, mean dose 2 mg/kg every 8 h intramuscularly or in a 20-min intravenous infusion. the mean serum half-lives in patients with creatinine clearances of >/=90 ml/min and 60 to 90 ml/min were 3.2 and 3.4 h, respectively. in patients with serum creatinines of </=1.4 mg/100 ml and creatinine clearances of >/=60 ml/min, mean serum levels were 9.0 and 1.2 mug/ml, respectively, 5 to 15 min and 7.5 h post-intravenous infusion, and 7. ...1978666303
relative importance of enterotoxigenic and invasive enteropathogenic bacteria in infantile diarrhoea.swedish children and adults (648 patients) with acute diarrhoea were investigated for enterotoxigenic strains in stool cultures. a total number 74 strains were isolated from 28 patients and assayed in the rabbit intestinal loop test and the adrenal cell test. only three of the enterotoxigenic e. coli (etec) isolates belonged to classical enteropathogenic serotypes of e. coli (epec). two enterotoxigenic strains of proteus morganii, two of enterobacter hafniae and one of citrobacter freundii were ...1978735553
[plasmid determined lactose positive character in (enterobacter hafniae) and (proteus morganii) (author's transl)].the atypical lactose positive characters have been found to be plasmidmediated in 6 strains of enterobacter hafniae and 2 strains of proteus morganii. one of the p. morganii plasmid possesses the fi+ character, all the other plasmids belonging to the fi-group. both plasmids of p. morganii could be transduced and remained then readily transferably by conjugation. two of the e. hafniae plasmids were found unstable when harboured in salmonella typhi grown at 40 degrees centigrade, when they were st ...1976786102
infectious arthritis resulting from enterobacter cloacae and enterobacter hafniae. 1977891089
bactericidal and opsonic activities of normal sheep serum against gram-negative bacteria.the bactericidal and opsonic activities of normal sheep serum against 9 smooth and 4 rough strains of gram-negative bacteria were measured. three smooth strains-escherichia coli 3662, salmonella typhimurium, and salmonella gallinarum-were resistant to the bactericidal action of normal sheep serum with or without complement. six smooth strains-e coli o78:k80(b), salmonella arizonae, proteus inconstans, klebsiella pneumoniae, salmonella stanley, and salmonella abortus-equi-were moderately sensitiv ...19751098532
tryptophan biosynthetic pathway in the enterobacteriaceae: some physical properties of the enzymes.several physical properties of the first four enzymatic activities of the tryptophan pathway were examined using gel filtration and ion exchange chromatography. five different patterns were noted. differences in the anthranilate synthetase (as) and phosphoribosylanthranilate transferase (prt) defined these patterns. in all the organisms studied phosphoribosylanthranilate isomerase and indoleglycerol phosphate synthetase co-eluted from both diethylaminoethyl-cellulose and g-200 and thus probably ...19751116988
proceedings: the effect of tris on the response of various isolated, mammalian preparations to nerve and drug stimulation. 19751151799
[a case of parieto-pulmonary acquired shunt presenting with hemoptysis (author's transl)].the authors report the case of a 52 year old man with arteriovenous shunt developing from the 7th left intercostal artery, revealed by hemoptysis and proved by intercostal selective arteriography. the patient had a past history of serofibrinous pleurity 29 years previously and had undergone paracentesis at this level. the origin of this shunt is then discussed.19751151917
[effect of an oxygen-vitamin complex on general and immunological reactivity of irradiated animals]. 19751153721
derivative absorption spectrophotometry of native proteins. 19751169006
[effectiveness of the influenza vaccination]. 19751196243
effect of neuraminidase treatment on the biological activity of highly purified ovine fsh and lh in hypophysectomized immature male and female rats.highly purified ovine fsh and lh were treated with neuraminidase to remove sialic acid and the desialylated derivatives were examined for biological activity in hypophysectomized immature male and female rats. the male rats were hypophysectomized at 22 days of age and beginning on day 25 were injected sc twice daily for 4 days with native or neuraminidase-treated fsh (total dose, 15 or 60 mug) or lh (12 mug). the ventral prostates, seminal vesicles, and testes were then removed and weighed, and ...19761248439
renaturation of bacteriophage lambda dna. determination of the optimal renaturation conditions using a single-strand-specific dnase and alkaline-sucrose-gradient assay system.reannealed hybrid molecules of wild-type bacteriophage lambda dna were prepared in aqueous solutions of formamide at a variety of nacl concentrations at both room temperature ( 22 degrees c) and 37 degrees c. treatment of the hybrid dna molecules with the single-strand-specific nuclease s1 from aspergillus oryzae followed by alkaline sucrose gradient sedimentation was used to monitor the extent and fidelity of hybridization. the optimal renaturation conditions at room temperature were found to b ...19761248479
dna relatedness among species of enterobacter and serratia.species of enterobacter and serratia were examined for deoxyribonucleic acid relatedness to klebsielleae, to atypical erwiniae, and to other members of enterobacteriaceae. deoxyribonucleic acid hybridization and then hydroxyapatite chromatography was the technique used to assess relatedness. strains of enterobacter cloacae formed two separate hybridization groups that correlate with the presence or absence of yellow pigment. pigmented e. cloacae were 75-100% related, but they were only 40-50% re ...19761260520
studies on immunity in mice immunized with outer membrane proteins of hafnia.nonspecific protection induced in mice after administration outer membrane proteins of hafnia alvei against infection with homologous and heterologous bacteria was transferred into other mice with lymphocytes isolated from spleens of mice immunized with outer membrane proteins. it was also found that mice sensitized with outer membrane proteins derived from h. alvei or with living bacteria induced in animals delayed hypersensitivity (dth) in homologous and heterologous systems. the observed type ...19921299169
some biological properties of outer membrane proteins of hafnia.outer membrane proteins (omp) isolated from four antigenically different strains of hafnia alvei were tested for the toxicity, pyrogenicity, ability to induce shwartzman reaction as well as for their influence on the leukocyte system. ld50 doses for the studied preparations determined on inbred mice were 18, 20, 28 and 34 mg/kg. these differences in the toxicity of the preparations were reflected in manifestation of shwartzman reaction; more toxic preparations induced strongest necrohemorrhagic ...19921299170
sharing of virulence-associated properties at the phenotypic and genetic levels between enteropathogenic escherichia coli and hafnia alvei.seven strains of hafnia alvei isolated from diarrhoeal stools of children resembled enteropathogenic escherichia coli (epec) in that they produced attaching-effacing (ae) lesions in rabbit ileal loops and fluorescent actin staining in infected hep-2 cells. in addition, a dna probe from a chromosomal gene required by epec to produce ae lesions, hybridised to chromosomal dna from all seven h. alvei strains. these findings indicate that there is a sharing of virulence-associated properties at the p ...19921433251
analysis of polar lipids from some representative enterobacteria, plesiomonas and acinetobacter by fast atom bombardment-mass spectrometry.fast atom bombardment-mass spectrometry (fab-ms) was used to analyse lipid extracts of bacteria to assess its usefulness for analysing anionic phospholipids of potential chemotaxonomic value. the following micro-organisms were tested: acinetobacter calcoaceticus, acinetobacter sp., citrobacter freundii, enterobacter cloacae (2 strains), escherichia coli (3 strains), hafnia alvei, klebsiella oxytoca, klebsiella pneumoniae, morganella morganii, plesiomonas shigelloides, proteus mirabilis (3 strain ...19921447059
in vitro activity and beta-lactamase stability of ljc 10,627.the in vitro activity of ljc 10,627, a new carbapenem, was compared with those of imipenem, cefotaxime, ceftazidime, and gentamicin. ljc 10,627 inhibited 90% of escherichia coli, klebsiella pneumoniae, klebsiella oxytoca, enterobacter aerogenes, enterobacter agglomerans, enterobacter cloacae, hafnia alvei, citrobacter freundii, citrobacter diversus, proteus mirabilis, morganella morganii, proteus rettgeri, serratia marcescens, pseudomonas cepacia, salmonellae, shigellae, aeromonas, and yersiniae ...19921510436
disruption of the gene encoding the secreted acid protease (acp) in the yeast candida tropicalis.the gene for the secreted acid protease (acp), a potential virulence factor of candida species, was inactivated in candida tropicalis by gene disruption. the disruption was performed by cotransformation of an ade2 c. tropicalis mutant with a linear dna fragment carrying a deletion in acp, and the replicative vector pmk16 which carries a selectable ade2 gene marker. few of the transformants exhibited lower protease secretion levels and were shown to have one deleted and one unaffected acp copy, s ...19921526448
ru 29 246, the active compound of the cephalosporin-prodrug-ester hr 916. i. antibacterial activity in vitro.the aminothiazolyl-cephalosporin ru 29 246 is the active metabolite of the prodrug-pivaloyl-oxyethyl-ester hr 916. ru 29 246 in vitro activity includes a wide range of clinically relevant bacterial pathogens. against methicillin-sensitive staphylococci ru 29 246 (mic90 of 0.25 approximately 2 micrograms/ml) was clearly more active than cefaclor, cefuroxime, cefpodoxime, cefixime and ceftibuten, but slightly less active than cefdinir. ru 29 246 inhibited hemolytic streptococci of the serogroups a ...19921592683
daily rhythmicity of the rat acoustic startle response.we have measured the acoustic startle response (asr) amplitude and latency in rats housed in a 12:12 light:dark (ld) cycle. the response amplitudes to eliciting stimuli (es) of 110 db or 120 db (white noise) were significantly higher (nearly two-fold) during d than during l. similar, but nonsignificant, trends were also observed at es intensities of 90 db or 100 db. while some significant ld asr latency differences were observed, we cannot ascribe them to the photoperiodic phase at this time. th ...19921594689
[colitis and hafnia alvei]. 19921602859
acute bacterial gastroenteritis caused by hafnia alvei. 19921623093
chiral linear hydroxamates as biomimetic analogues of ferrioxamine and coprogen and their use in probing siderophore-receptor specificity in bacteria and fungi.linear hydroxamate derivatives, possessing chiral alpha-amino acid moieties, were synthesized and their iron transport activities were studied in bacteria and fungi. no growth-promoting activity could be detected in the gram-positive hydroxamate-auxotroph aureobacterium flavescens jg9. however, gram-negative enterobacteria, such as escherichia coli, pantoea agglomerans and hafnia alvei were able to utilize iron from these analogues. uptake of 55fe-labeled analogues was inhibited by sodium azide, ...19911657086
in vitro activity and stability against novel beta-lactamases of investigational beta-lactams (cefepime, cefpirome, flomoxef, sce2787 and piperacillin plus tazobactam) in comparison with established compounds (cefotaxime, latamoxef and piperacillin).the therapeutic perspectives of flomoxef, sce 2787, cefpirome, cefepime, latamoxef, cefotaxime and of piperacillin plus tazobactam were comparatively evaluated by their in vitro activity against 1119 clinical isolates of 83 bacterial species. escherichia coli, klebsiella spp. enterobacter sakazakii, proteus spp. and shigella spp. were about equally susceptible to the cephalosporins (mic90: 0.06 to 0.5 mg/l), while the mic90 for piperacillin plus tazobactam was between 2 and 16 mg/l. enterobacter ...19911664418
monoclonal antibodies to quebec strain (q17) of bovine rotavirus.fourteen monoclonal antibodies (mabs) against "quebec" strain (q17) of bovine rotavirus were isolated and characterized. four were specific for viral protein vp7 and ten were specific for viral protein vp6. five different isotypes were represented by this group of antibodies. all of the anti-vp6 and none of the anti-vp7 antibodies were sensitive to the effects of periodate on their antigen. the antibodies could be separated into three groups based on their relative resistance to the dissociation ...19901698323
[diagnosis and therapy of viral hepatitis]. 19911713243
structural studies on hafnia alvei 114-60 o-antigen.the structure of hafnia alvei 114-60 o-antigen has been established using sugar and methylation analyses as well as 1h-nmr spectroscopy. the results obtained proved that the repeating unit of hafnia alvei 114-60 o-antigen is identical to that of hafnia alvei atcc 13337 standard strain and has the following structure: [formula: see text]19901718234
the effect of exposure to impulse x rays on normal and epileptic activity in rabbit brain.exposure to x rays (20 impulses of 4 hz frequency, total dose 0.6-1.1 mgy) increased the epileptic activity of a focal area, which was produced in the visual cortex of rabbit brain by freezing with liquid nitrogen, and by stimulating with flashes of light at frequencies of 5-6 hz. the number of seizure complexes during photostimulation for 5 s increased by 80% compared with the initial level, and this effect continued for 15 min. in control animals (with no epileptic foci), a decrease was observ ...19911797142
the effect of exposure to impulse x rays on normal and epileptic activity in rabbit brain.exposure to x rays (20 impulses of 4 hz frequency, total dose 0.6-1.1 mgy) increased the epileptic activity of a focal area, which was produced in the visual cortex of rabbit brain by freezing with liquid nitrogen, and by stimulating with flashes of light at frequencies of 5-6 hz. the number of seizure complexes during photostimulation for 5 s increased by 80% compared with the initial level, and this effect continued for 15 min. in control animals (with no epileptic foci), a decrease was observ ...19911797142
[clinical significance of the isolation of hafnia alvei from the feces of children with acute gastroenteritis]. 19911822161
identification of salmonellae with the 4-methylumbelliferyl caprilate fluorescence test.we have tested 750 salmonella strains and 130 strains of other species of the family enterbacteriaceae with the 4-methylumbelliferyl caprilate reagent (mucap) test. the mucap test is a fluorescence test for rapid identification of salmonella strains. the non-salmonella strains were strains sent for identification as suspected salmonella strains and thus have phenotypes similar to those of salmonella strains. all 748 tested salmonella strains of subgroups i, ii, iii, and iv were positive in the m ...19911774276
[hafnia alvei septicemia with shock and dic in an adult with postoperative lung cancer].in japan, we experienced the first case of hafnia alvei septicemia with shock and disseminated intravascular coagulation (dic) in an adult with postoperative lung cancer. a 63 year-old male, who had been followed up in our department since 1987, was admitted to our hospital with the complaints of fever, hemoptysis and dyspnea on june 25, 1989. after admission, he was treated with sulbactam/cefoperazone 4 g/day intravenously for suspicion of respiratory-tract infection. after antibiotic administr ...19911761901
expression of a bacterial lysine decarboxylase gene and transport of the protein into chloroplasts of transgenic tobacco.a possible approach for altering alkaloid biosynthesis in plants is the expression of genes encoding key enzymes of a pathway such as lysine decarboxylase (ldc) in transgenic plants. two strategies were followed here: one focused on expression of the gene in the cytoplasm, the other on subsequent targeting of the protein to the chloroplasts. the ldcgene from hafnia alvei was therefore (a) placed under the control of the 1' promoter of the bidirectional tr promoter from agrobacterium tumefaciens ...19911883998
esp3i--a novel type iis restriction endonuclease from hafnia alvei that recognizes the sequence 5'-cgtctc(n)1/5-3'. 19911923776
l-prolineaminopeptidase activity as a tool for identification and differentiation of serratia marcescens, serratia liquefaciens and hafnia alvei strains.s. marcescens (316 strains), s. liquefaciens (10 strains) and hafnia alvei (20 strains), in contrast to 18 other enterobacteriaceae species, hydrolyzed l-proline-4-nitroanilide within 30 min at 37 degrees c. in this way, rapid identification of these species is possible. the substrate is applied in solution or in a paper disc. the substrate optimum of hafnia alvei strains proved to be 16 times lower than that of the serratia spp. application of a tenth of the substrate concentration necessary fo ...19911930558
o-specific polysaccharide of hafnia alvei lipopolysaccharide isolated from strain 1211. structural study using chemical methods, gas-liquid chromatography/mass spectrometry and nmr spectroscopy.the hafnia alvei strain 1211 o-specific polysaccharide is composed of 3-amino-n-(d-3'-hydroxybutyryl)-3,6-dideoxy-d-galactose, n-acetyl-d-galactosamine, n-acetyl-d-glucosamine and d-glucose (1:1:2:2). on the basis of sugar and methylation analyses, smith degradation, and one- and two-dimensional 1h- and 13c-nmr spectroscopy, the polysaccharide was shown to be an o-acetylated polymer of the repeating hexasaccharide unit, ----2d(4-oac)fucp3nacyl beta 1----6dglcpnac alpha 1---- (dglcp beta 1----3)4 ...19911716201
[the identification of lactose-negative enterobacteria].study of the morphologic and enzymatic characteristics of 10 strains of lactose-negative enterobacteria, sent to the l. a. tarasevich state institute for control and standardization as b. paracoli in order to determine their taxonomic status in accordance with the 1974 and 1984 bergey classifications has referred 9 of these strains to hafnia alvei.19901705611
induction of the alka gene of escherichia coli in gram-negative bacteria.a broad-host-range plasmid containing a fusion of the alka and lacz genes of escherichia coli was introduced into various aerobic and facultative gram-negative bacteria--33 species belonging to 19 genera--to study the induction of expression of the alka gene by alkylating agents. the bacteria included species of the families enterobacteriaceae, pseudomonadaceae, rhizobiaceae, vibrionaceae, neisseriaceae, rhodospirillaceae, and azotobacteraceae. results obtained show that all bacteria tested, exc ...19911938974
pathogenicity and antigenic components of excysted giardia lamblia isolated from patients in riyadh, saudi arabia.giardia lamblia cysts were isolated from patients in riyadh, saudi arabia. cysts and trophozoites (from axenically excysted cysts) were given orally by gavage to mice to establish the pathogenicity of the riyadh isolate. there was no effect of varying the dose of administered parasite on parasite excretion or morbidity. a typical pathologic pattern of giardiasis was demonstrated by histologic methods and electron microscopy. antigenic components of the riyadh isolate were compared with the portl ...19911951852
production and characterization of a monoclonal antibody specific for enterohemorrhagic escherichia coli of serotypes o157:h7 and o26:h11.a monoclonal antibody (mab 4e8c12) specific for escherichia coli o157:h7 and o26:h11 was produced by immunizing balb/c mice with a rough strain of e. coli o157:h7. the antibody reacted strongly by a direct enzyme-linked immunosorbent assay with each of 36 strains of e. coli o157:h7. no cross-reactivity was observed with strains of salmonella spp., yersinia enterocolitica, shigella dysenteriae, proteus spp., escherichia hermanii, klebsiella pneumoniae, campylobacter jejuni, serratia marcescens, c ...19911993773
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