profiles for ph, temperature, and dissolved o2 levels in enzyme production: monitoring in small-scale fermentors.the profiles thus established may be utilized for investigations of an organism's relationship to its microenvironment including metabolic shifts and pathways. areas of maximum respiratory activity, enzyme production, enzyme degradation, and attainment of the stationary phase are quite evident; however, duration and magnitude of the various phenomena may change with nutrient, temperature, and aeration efficiency. practical application of this simplified method would include: a) determination of ...19751123
physicochemical and antigenic properties of the erwinia carotovora bacteriophages. 19752849
relationship between the magnitude of km and ph for l-asparaginase.the dependency of l-asparagine hydrolysis rate on l-asparagine concentration in the presence of l-asparaginases from e. coli and erw. carotovora is studied in a broad ph range. km values are calculated from the data obtained. it is found that km insignificantly depends on ph value with the ph range of 5-9 for both asparaginases. sharp km maximum is observed at ph greater than 9 in both cases. the maximum position does not coinside with enzyme isoelectric points and with the region of the substra ...19766070
polygalacturonic acid trans-eliminase in the osmotic shock fluid of erwinia rubrifaciens: characterization of the purified enzyme and its effect on plant endopolygalacturonic acid trans-eliminase (ec, released by osmotic shock of erwinia rubrifaciens cells, has been purified to near homogeneity (3, 100-fold) by column chromatography on diethylaminoethyl-cellulose, phosphocellulose, and hydroxyapatite-cellulose followed by isoelectric focusing. it has a molecular weight of 41,000, s20,w of 3.09s, an isoelectric point of ph 6.25, ph optimum of 9.5, and a temperature optimum of 37 c and requires ca2+ with an optimum concentration of 0.5 ...19766435
detection of amino acid decarboxylases in bacteria of the genus erwinia by a ph-sensitive method. 19767730
effects of asparagine synthetase inhibitors on asparaginase resistant tumors. 197720104
bacterial 2,3-butanediol dehydrogenases.enterobacter aerogenes, aeromonas hydrophila, serratia marcescens and staphylococcus aureus possessing l(+)-butanediol dehydrogenase produced mainly meso-butanediol and small amounts of optically active butanediol; acetobacter suboxydans, bacillus polymyxa and erwinia carotovora containing d(-)-butanediol dehydrogenase produced more optically active butanediol than meso-butanediol. resting and growing cells of these organisms oxidezed only one enantiomer of racemic butanediol. the d(-)-butanedio ...197825056
studies of relationship among terrestrial pseudomonas, alcaligenes, and enterobacteria by an immunological comparison of glutamine synthetase.antibody to purified glutamine synthetase from escherichia coli was prepared and used for an immunological comparison of glutamine synthetases from species of salmonella, citrobacter, enterobacter, serratia, proteus, erwinia, aeromonas, pseudomonas, acinetobacter, xanthomonas, alcaligenes, and paracoccus. the results of ouchterlony double diffusion experiments and quantitative microcomplement fixation studies indicated that the amino acid sequence of this enzyme was highly conserved in different ...197831146
production of cellulase (cx) by different species of erwinia.the tested isolates of erwinia chrysanthemi (corn pathotype) and e. carotovora constitutively produce high levels of cellulase(s) (cx) in presence or absence of carboxymethyl-cellulose (cmc) as substrate in the medium. the tested isolates of e. atroseptica produced high levels of cellulase when grown in presence of both carboxymethyl-cellulose (cmc) and sucrose, low levels in presence of carboxymethyl-cellulose alone, and traces of cellulase(s) in presence of sucrose alone. the activity of cellu ...197938608
letter: toxicity of asparaginases. 197548918
induction of avirulent variants in erwinia stewartii by incubation at supraoptimal temperatures.high temperatures (37 degrees c) induced non-pigmented, and (or) small colony variants in some erwinia stewartii strains. the former differed from the parent strain serologically and in having lost virulence to zea mays. the small colony variants retained phytopathogenicity.197551679
immunological relationships of bacterial l-asparaginases.rabbit antisera against l-asparaginase preparations from escherichia coli, erwinia carotovora, citrobacter sp. and chromobacterium violaceum showed on immunoelectrophoresis that only the enzymes from e. coli and citrobacter are immunologically related. purified preparations had to be used to determine the immunological cross-reactions. immunoelectrophoresis at different ph values yielded the zero mobility points of the enzymes. the activity of the er. carotovora preparation was enhanced up to fo ...197654404
[various properties of virulent and moderate erwinia carotovora phages].a bacterial culture of e. carotovora 8638 lysed by virulent phage 62 after treatment with uv rays produces a moderate phage. the virulent and moderate viruses, antigenically unrealted, have a similar prolonged latent period (100 min) and the period of lysis (60 min). they also differ in the sizes of the capsids and the length of processes which are 750+/-30 and 2000+/-50a in the virulent phage and 600+/-30 and 1500+/-50a, in the moderate phage, respectively. the sedimentation constants are 602 a ...197664030
[evidence for a bacteriocin in erwinia uredovora]. 197664197
[serological study of erwinia aroideae (townsend) holland and e. carotovora (jones) holland isolated from hyacinths and callas]. 197771650
[structural components of adenvoiruses and other dna-genome viruses]. 197878433
characterization of a bacteriocin produced by erwinia uredovora [proceedings]. 197993940
serologic studies of the maize stalk rot pathogen erwinia carotovora f. sp. zeae.with the help of serological techniques, namely microprecipitin, agglutination, and gel diffusion, sixteen isolates of maize stalk rot pathogen were proved to be identical. serological techniques have thus been utilized as an additional tool for identifying the pathogen erwinia carotovora f. sp. zea. it was also established that the pathogen does not perpetuate in the seed, either externally of internally. it could, however, be found through serological tests that infected ears carried the patho ...197896628
exposure to dust-borne bacteria in agriculture. ii. immunological order to investigate whether high exposure to erwinia herbicola causes sensitization in grain handlers, immunological tests (agargel precipitation, complement fixation, passive cutaneous anaphylaxis, and intradermal) with the extracts of these bacteria were performed in different groups of grain workers and in other groups of population. tests with extracts of grain dust and other microorganisms were also performed. grain workers showed in all the tests high incidence of positive reactions to ...1978101155
[sensitivity of causative agents of lupine bacteriosis to oxine group compounds]. 1978102908
homology of the gene coding for outer membrane lipoprotein within various gram-negative bacteria.the mrna for a major outer membrane lipoprotein from escherichia coli was found to hybridize specifically with one of the ecori and one of the hindiii restriction endonuclease-generated fragments of total dna from nine bacteria in the family enterobacteriaceae: e. coli, shigella dysenteriae, salmonella typhimurium, citrobacter freundii, klebsiella aerogenes, enterobacter aerogenes, edwardsiella tarda, serratia marcescens, and erwinia amylovora. however, among the enterobacteriaceae, dna from two ...1979104972
the effect of chemical modification of l-asparaginase on its persistence in circulating blood of animals. 1979110336
[properties of enterobacter agglomerans strains isolated from different sources (author's transl)]. 1975127663
effect of three nematicides on the growth of some phytopathogenic bacteria and fungi.the effect of three nematicides, aldicarb, fensulfothion, and phenamiphos at four concentrations (1, 5, 25, and 125 ppm) was tested on the growth of five bacteria, agrobacterium tumefaciens, corynebacterium fascians, erwinia carotovora, pseudomonas solanacearum, and streptomyces scabies and four fungi, fusarium oxysporum f. sp. vasinfectum, fusarium solani, rhizoctonia solani, and sclerotium bataticola. of the bacteria, p. solanacearum was most affected by the chemicals at all concentrations, wh ...1977143857
differential toxicities of mercury to bacteria and bacteriophages in sea and in lake water.mixtures of anionic hgcl3-/hgcl4(2)-complexes were less toxic to terrestrial bacteria (erwinia herbicola, agrobacterium tumefaciens), to marine bacteria (acinetobacter sp., aeromonas sp.), and to bacteriophages (phi 11 m 15 of staphylococcus aureus and p1 of escherichia coli) than were equivalent concentrations of hg as cationic hg2+. the toxicity of 1 ppm hg to a. tumefaciens. aeromonas sp., and phi 11 m 15 was less in seawater than in lake water. inasmuch as the hg-cl species are formed in env ...1979161510
"self-catabolite repression" of pectate lyase in erwinia carotovora.the induction of pectate lyase of erwinia carotovora was repressed by a high concentration of its inducer. the concomitant addition of cyclic adenosine 3',5'-monophosphate reversed this repression.1979217862
a convenient coupled assay for tyrosine phenol lyase (deaminating) [proceedings]. 1979225229
properties of the purified penicillin v-acylase of erwinia aroideae. 1975234389
partial purification and properties of a beta-glucosidase from erwinia herbicola y46.a constitutive beta-glucosidase of erwinia herbicola y46 was studied as a prerequisite to an assessment of its significance in the release of bacteriotoxic aglycones from plant beta-glucosides, and the possible effects of the aglycones on the course of such plant diseases as "fire-blight". the enzyme was purified 86.5-fold from crude extracts of cells grown on yeast beef broth. ammonium sulfate precipitation, deae-cellulose fractionation, and gel filtration through sephadex g-100 resulted in a p ...1975235359
comparative study on conformational stability and subunit interactions of two bacterial asparaginases.the denaturation and reconstitution of erwinia carotovora and escherichia coli l-asparaginases has been followed by optical rotatory dispersion, circular dichroism and analytical ultracentrifugation. denaturation in urea results in dissociation of the native enzyme (mol. wt. 140 000 approx.) to produce unfolded subunits (mol. wt. 35 000 approx.); the erwinia l-asparaginase subunits can be refolded by dilution or dialysis in alkaline conditions, ph 10.5, without aggregation to the active tetramer ...1975237538
the isolation and characterization of a temperate phage, y46/(e2), from erwinia herbicola y40.a temperate phage was induced from exponential phase cells of erwinia herbicola y46 by treatment with mitomycin c. the phage was purified by single plaque isolation, and produced in bulk by successive cultivation in young cultures of e. herbicola y 178. phages were concentrated from culture filtrates by rate zonal centrifugation and resuspension in 0.02 m tris buffer, ph 7.2, twice, yielding suspensions of about 5 times 10(11) pfu/ml. purification was achieved by centrifugation in buffered sucro ...1975238732
taxonomy of the genus hundred and fifty-six strains of serratia and related bacteria including representatives of enterobacter liquefaciens, enterobacter cloacae, enterobacter aerogenes, erwinia carotovora, erwinia chrysanthemi, erwinia herbicola and erwinia nimipressuralis were studied using 223 morphological, physiological, biochemical and carbon source utilization tests. the results were subjected to computer analysis. at the 80% similarity level all strains, except two, grouped into eight phenons representing ...1977319202
characterization of enterobacter agglomerans (erwinia spp.) from clinical specimens.the biochemical reactions of 18 anaerogenic and 45 aerogenic isolates of enterobacter agglomerans are described and used for subdivision in the biogroups suggested by ewing & fife. the presence of special cultural characteristics (symplasmata and biconvex bodies) was also recorded and was found to be unrelated to formation of gas from glucose. previous authors have reported such structures in anaerogenic cultures only. the amount of gas produced varied to a great extent between strains and also ...1977320820
[results of an experimental study of the antitumor activity of 1-asparaginase from e. coli and erw. carotovora].the antitumour activity of the preparations of l-asparaginase from e. coli and erw. carotovora with respect to lymphadenosis l-5178 and yorker's carcinosarcoma (ascitic cariants) has been established. no difference in antitumour efficacy of the preparation of l-asparaginase obtained from e. coli and erw. carotovora was noted.1977320935
application and standardization of various procedures for inoculation of maize by erwinia carotovora f. sp. zeae.hypodermic syringe and toothpick methods were best for evaluating pathogenicity of the maize plant against erwinia carotovora f. sp. zeae, both in glass house and in the field. the portion of the stalk above the ground was best suited for reproducing the disease symptoms. leaf and leaf whorl did not show any rotting hero. seed inoculation showed least mortality percentage. root inoculation was suitable only for young seedlings. cotyledons, as an organ for inoculation for getting best reproducibl ...1977324175
transfer of episome f'lac+ and chromosomal trp+ genes from erwinia amylovora to salmonella typhimurium.the f'lac+ episome of escherichia coli origin was transferred by conjugation with frequencies of 10(-7) to 10(-5) from erwinia amylovora to 14 out of 15 salmonella typhimurium trp female parents. the chromosomal trp+ genes were transferred with frequencies of 10(-7) to 10(-6) only to one trpb and 2 trpd female parents, which have a point mutation in the 2nd and fourth structural genes, respectively, of the tryptophan operon. the transferred male trp+ genes became integrated at the selected sites ...1977330120
bacterial phytotoxins.representatives of all of the major genera of plant disease-causing bacteria have been shown to produce one or more phytotoxins. toxicological studies on the bacterial diseases of the major food crops have not been extensive. bacterial phytotoxins belong to a number of classes of organic substances, including peptide, derivatized amino acid, polysaccharide, and glycopeptide. the mechanism of action of these compounds is largely unknown, as is their origin and importance in the disease syndrome. ...1977334039
enzymatic degradation of polygalacturonic acid by yersinia and klebsiella species in relation to clinical laboratory scored by several specified plating procedures, clinical and environmental strains of yersinia enterocolitica, yersinia pseudotuberculosis, and klebsiella pneumoniae "oxytocum" showed detectable, albeit generally weak, ability to digest polygalacturonic (pectic) acid. none of these bacterial strains had the vigorous and rapid pectolytic activity on these polygalacturonic acid-containing media that is typical of soft-rot erwinia species, although some of the oxytocum strains came fairly close. ...1977334794
purification and properties of a third form of anthranilate-5-phosphoribosylpyrophosphate phosphoribosyltransferase from the enterobacteriaceae.anthranilate-5-phosphoribosylpyrophosphate phosphoribosyltransferase was purified from the bacterium erwinia carotovora, a member of the enterobacteriaceae. the enzyme was homogeneous according to the criteria of gel electrophoresis and nh2-terminal amino acid sequence analysis. the molecular weight of the enzyme as determined on a calibrated sephadex g-200 column was 67,000 +/- 2,000. sodium dodecyl sulfate-polyacrylamide gels gave a subunit molecular weight of 40,000 +/- 1,000, suggesting that ...1978338606
toxic and immunodepressive effects of l-asparaginase from e. coli and from erwinia carotovora following chronic administration in rats.the effects of the administration of l-asparaginase from e. coli and erwinia carotovora were studied in rats treated for 90 days with 800 or 3200 iu/kg body weight. the studies included overall toxicity on the liver, pancreas, and enteric mucosa as evaluated by both opitcal and electron microscopic examination, biochemical findings, behaviour of igm-hemolysin producing cells, and antias-paraginase antibody production. the toxic effect and the immunodepressive activity appeared rather early, tend ...1977341899
characterization and clinical identification of enterobacteriaceae by dna hybridization. 1978345354
[f'colvcolbtrpcys plasmid in erwinia aroideae].erwinia aroideae carries a cryptic plasmid with 30 s sedimentation coefficient. plasmid f'colvcolbtrpcys does not dissociate in e. aroideae and is replicated under stringent control since the number of plasmid copies per chromosome does not exceed one. the behaviour of f'colvcolbtrpcys plasmid in e. aroideae is characterized by (1) instability observed at both spontaneous and after eb treatment, (2) depression of plasmid genes that determine colicin synthesis.1978346444
[infections due to chromobacterium, serratia, erwinia and flavobacterium (the so-called chromobacterioses)]. 1978361381
trypsin peptide patterns of tryptophan synthase beta2 protein among four species of the enterobacteriaceae.the tryptophan synthase beta 2 protein (ec of escherichia coli, enterobacter aerogenes, serratia marcescens, and erwinia carotovora was purified and compared. two-dimensional total peptide patterns for each of the four beta2 proteins obtained after digestion with trypsin showed that approximately three quarters of the total peptides are common to all four peptides. examination of only arginine-containing peptides showed that approximately half of these peptides are common. from a compa ...1978361723
[transmission of f'lac plasmid from escherichia coli k-12 to bacteria of the genus erwinia].the f'lac plasmid was transferred by conjugation from escherichia coli k-12 w1655 to 21 lac- strains of erwinia spp. (5.2 . 10(-6) to 6.8 . 10(-2) lac+ exconjugants per donor cell). erw. herbicola and erw. chrysanthemi were the better recipients than others. the degree of the stability of lac+ genes in erwinia exconjugants depends on the strains. stable exconjugants of erwinia, which harbored f'lac plasmid, were able to utilize lactose, to transfer lac genes by conjugation to erwinia spp. and e. ...1978363511
synthesis and excretion of polygalacturonic acid trans-eliminase in erwinia, yersinia, and klebsiella species. 1979371775
[expression of the gene for tetracycline resistance of plasmids r6 and rp4 in bacteria of the family enterobacteriaceae].it was found that manifestation of the tetracycline resistance gene depended on the type of the plasmid containing the gene. the tetracycline resistance gene was subject to less repression in plasmid r6 than in plasmid rp4. sensitivity of the initial plasmid-free bacteria varied within lower dose ranges than that of the plasmid-carrying strains. regulation of the tetracycline resistance gene manifestation in the given plasmid may change in different bacterial hosts, i.e. in different cytoplasmic ...1979375821
improvement in the therapeutic, immunological, and clearance properties of escherichia coli and erwinia carotovora l-asparaginases by attachment of poly-dl-alanyl peptides.the chemical modification of both escherichia coli and erwinia carotovora asparaginases by a dl-alanine-n-carboxyanhydride polymerization technique produced modified enzymes which had greater protease stability, retained most of their catalytic activity, and demonstrated a 7- to 10-fold prolongation in plasma clearance properties in normal mice and rats. concomitantly, plasma substrate depletion was also extended 5 to 13 days longer for the modified as compared with the native enzymes. for prepa ...1979376113
exocellular, microbial polysaccharides. 1979380276
nitrogen fixation in a biotype of erwinia herbicola resembling escherichia coli. 1979383675
[acceptance and transfer of plasmid rts1 by bacteria of the genus erwinia].the ability of 13 erwinia strains to accept, to inherit and to transmit the rts1 factor by conjugation was studied. 11 strains accepted the rts1 factor from escherichia coli k-12 csh-2 with the frequency of about 10(-7)--10(-3). the rts1 factor was genetically stable in the erwinia cells and was not eliminated by acriflavine and under the temperature of 37 and 42 degrees c. all the r+ exconjugants were characterized with more high degree of the resistance of kanamycin than e. coli cells harbouri ...1979387520
lysinoalanine utilization by erwinia chrysanthemi and escherichia coli. 1979387557
nitrogen fixation associated with 'park' kentucky bluegrass (poa pratensis l.).associative nitrogen fixation in kentucky bluegrass (poa pratensis l.) turfs inoculated with five nitrogen-fixing bacterial isolates was evaluated using the acetylene reduction assay and nitrogen accumulation as indicators of fixation. 'park' and 'nugget' kentucky bluegrass turfs were grown in controlled environment chambers and inoculated with klebsiella pneumoniae (w-2, w-6, and w-14), erwinia herbicola (w-8), and enterobacter cloacae (w-11). 'park' inoculated with k. pneumoniae (w-6) had sign ...1979394819
influence of culture medium of the fatty-acid profile in enteric bacteria.enteric bacteria having a high content of cyclopropane fatty acids steeply increase their synthesis when grown on insufficiently propitious culture media (meat-peptone agar or modified drobot'ko synthetic medium) as compared with bacteria grown under more favourable conditions (meat-peptone broth). simultaneously, a decrease in monounsaturated fatty acids and increase in palmitic acid are observed. one of the main factors underlying the change in the proportion of fatty acids in bacteria grown o ...1979399287
immunochemical comparison of phosphoribosylanthranilate isomerase-indoleglycerol phosphate synthetase among the enterobacteriaceae.the bifunctional enzyme of the tryptophan operon, phosphoribosylanthranilate isomerase-indoleglycerol phosphate synthetase (prai-ingps;ec, was characterized by an immunochemical study of six representative members of the enterobacteriaceae: escherichia coli, salmonella typhimurium, enterobacter aerogenes, serratia marcescens, erwinia carotovora, and proteus vulgaris. prai-ingps was purified from e. coli, and antisera were prepared in rabbits. these antisera were utilized in quantitativ ...1977403178
numerical taxonomy of heavy metal-tolerant bacteria isolated from an estuary.a total of 230 strains of metal-tolerant bacteria from water and sediment samples collected in chesapeake bay were isolated on a medium containing cobalt, lead, mercury, or molybdenum. in addition, a set of 71 cultures were simultaneously isolated on glucose tryptone yeast extract agar medium without metals. twenty-three reference strains were also included in the numerical taxonomy study of these bacteria, bringing the grand total of strains examined to 324. all strains were examined for 112 bi ...1977411560
inducer of pectic acid lyase in erwinia carotovora. 1977413045
comparative immunological studies on arylsulfatase in bacteria of the family enterobacteriaceae: occurrence of latent arylsulfatase protein regulated by sulfur compounds and tyramine.the arylsulfatases of 21 strains of the family enterobacteriaceae were compared by measuring their enzymatic activities and immunological reactivities. enzyme formation under repressing, nonrepressing, and derepressing conditions was tested. antiserum prepared against pure arylsulfatase from klebsiella aerobgenes w70 was tested against the enzyme extracts from the strains using double diffusion, quantitative precipitation, and immunoelectrophoresis. no close relationship was found between arylsu ...1978415041
amino acid degrading enzymes for cancer therapy.the development of microbial enzymes for cancer therapy presents difficulties not commonly experienced with biological drugs. the development of the enzyme asparaginase from escherichia coli in the usa and of the serologically different asparaginase from the plant pathogen erwinia carotovora in this establishment, has not only added to the choice of antileukaemia drugs but also provided a valuable guide to the selection and development of new therapeutic enzymes. our own programme has led to the ...1977415922
enterobacteriaceae infections in man and animals and detection of their natural reservoirs in india.the study presents a summary of the results regarding the presence of enterobacteriaceae in domestic animals and birds, endeavouring to explain the origin of the infections. wild reptiles, amphibians, mammalia, rodents and birds were examined into the demonstration of enterobacteriaceae, whereby particular attention was paid to the following species: arizona, edwardsiella, citrobacter, enterobacter, serratia, yersinia and erwinia.1979452773
the post-harvest fruit rots of tomato (lycopersicum esculentum) in nigeria.a survey of the post-harvest fruit rot diseases of tomato was conducted in five states of nigeria. during severe infections, the diseases could cause 25% loss at harvest and 34% loss of the remaining product in transit, storage and market stalls; thus giving an overall loss of about 50% of the product. two types of rots, soft and dry were recognised. the soft rot was found to account for about 85% and the dry rot about 15% of the overall loss. erwinia carotovora, rhizopus oryzae, r. stolonifer, ...1979471028
[bacteriocins of genus erwinia--especially on the bacteriocins from e. carotovora (author's transl)]. 1979472330
[model trials for isolation of soft rot bacteria by media containing pectic substances (author's transl)].1. under aerobic conditions bacterial soft rots are caused in the most cases by pectolytic erwinia, pseudomonas, and bacillus spp. 2. using 7 soft rotting strains several selective media were tested for their ability to sure a quick and exact isolation and differentiation of these pathogens. by bile salt-lactose-medium all erwinia spp. under test could be isolated. for isolation of pectinolytic pseudomonas strains the d4 medium of kado and heskett was suitable as the best one. 3. by the use of s ...1979473958
significance of erwinia in the vagina as causative agents of urinary tract infections.the isolation of erwiniae from the vagina became positive just before the occurrence of erwiniuria. it is proposed that the appearance of erwiniae in the vagina is an important indicator in the prediction of urinary tract infection by erwiniae.1979494253
[biological properties of erwinia herbicola (duggeli) dye isolated from plants of the cucurbitaceous family]. 1979530119
gas chromatographic analysis of in situ cyclitol utilization by klebsielleae growing in redwood extracts.gas chromatographic analysis was employed to demonstrate in situ cyclitol utilization in aqueous extracts of redwood by isolates of klebsiella, enterobacter, and several other genera of gram-negative bacteria. in aqueous redwood extracts, all but one of the klebsiella and enterobacter isolates tested reached densities exceeding 5.0 x 10(6) cells/ml within 4 days, and all utilized pinitol and sequoyitol. other enteric bacteria did not utilize cyclitols in this extract. a defined minimal medium, c ...1979539819
head rot of seed cabbage in kulu valley and its control. 1979548524
metabolites from microbially infected potato. part 1. structure of phytuberin. 1977556731
[effect of erwinia carotovora bacteriophage 62 on various indexes of the immune system in animals with ehrlich carcinoma]. 1978567268
effect of three antagonists on the development of bacterial leaf streak of rice.among 11 epiphytic microorganisms one species each of pseudomonas, erwinia, and aspergillus were antagonistic to xanthomonas translucens subsp. oryzicola. symptoms of bacterial leaf streak did not develop when the antagonists were sprayed on rice leaves 24 h before inoculation. although the symptoms developed when the antagonists were applied 24 h after inoculation, the number of lesions and their length was significantly reduced over control. when the mixture of each antagonist and the pathogen ...1978567522
effect of dusts emitted by copper smelters on erwinia carotovara.the effect of the dust emitted by copper smelters on erwinia carotovora was examined. the dust contains considerable amounts of heavy metals which inhibited the growth and enzymatic activity of the bacterial cultures. the inhibition of dehydrogenase and protease activity was greater than that of the growth rate. the phenolase production and virulence of strain was also inhibited, depending on the doses of dust. calcium carbonate counteracted the toxic effect of dust, restoring enzymatic activity ...1977576108
enterobacteria of emerging pathogenic significance from clinical cases in man and animals and detection of toads and wall lizards as their reservoirs.a total of 416 samples comprising faecal samples from diarrhoeic cases of man, calves, sheep and goats, and urine samples from patients with urinary tract infections, were examined for the presence of enterobacteria of emerging pathogenic significance. citrobacter freundii from 20, c. intermedius biotype-a from four, serratia marcescens (serotype 05:h13, bactericin type 16) from one and erwinia herbicola from two human stool samples were isolated. only two urine samples yielded c. freundii. citr ...1978582096
a simple colorimetric method for determination of serum triglycerides with lipoprotein lipase and glycerol dehydrogenase.a simplified enzymic procedure to determine accurately serum triglycerides is described. serum triglycerides are hydrolyzed completely to free fatty acids and glycerol by lipoprotein lipase from pseudomonas fluorescens. the released glycerol is oxidized with glycerol dehydrogenase from erwinia aroideae in the presence of nad+, were the reduction of the enzyme-linked nad+ is coupled to the reduction of nitro blue tetrazolium as a chromogenic indicator with phenazine methosulfate serving as an int ...1977589792
microbial conversion of dl-2-amino-delta2-thiazoline-4-carboxylic acid to l-cysteine and l-cystine: screening of microorganisms and identification of products.microorganisms able to form l-cysteine from dl-2-amino-delta2-thiazoline-4-carboxylic acid (dl-atc), a chemical intermediate in the synthesis of dl-cysteine, were isolated from soil samples and classified as pseudomonas sp., pseudomonas cohaerens, p. desmolytica, and p. ovalis. thirteen l-cysteine-producing bacteria were also found in among 463 stock cultures representing 37 genera. these were achromobacter delmarvae. alcaligenes denitrificans, bacillus brevis, brevibacterium flavum, enterobacte ...1977596877
nitrogen fixation (acetylene reduction) associated with roots of winter wheat and sorghum in nebraska.root segments and root-soil cores (6.5-cm diameter) from fields and nurseries of winter wheat and sorghum were tested for n2 fixation by using the acetylene reduction assay. wheat samples (approximately 1,200) from 109 sites generally had low or no activity (0 to 3.1 nmol of c2h4 produced per h per g [dry weight] of root segments), even after 24 h of incubation. however, a commercial field of scout 66, located in western nebraska, exhibited appreciable activity (290 nmol of c2h4 produced per h p ...1978623458
[inhibition of erhlich carcinoma and lymphoma nk/ly development by bacteriophage 62]. 1978634168
isolation and characterization of hfr strains of erwinia amylovora.hfr strains (hfr 159 and its derivatives, hfr 160 and hfr 161) were constructed from erwinia amylovora icpb ea178 by introducing an escherichia coli f'his+ plasmid and then selecting for integration of f'his+ after treatment with acridine orange. the hfr strains were relatively stable upon repeated transfers on nonselective media. interrupted mating experiments and analyses of inheritance of unselected markers showed that his+ is transferred by hfr 159 as the proximal marker at a relatively high ...1978638897
purification and some properties of glycerol dehydrogenase from erwinia aroideae. 1978647848
[bacteriocinogenic activity of strains of the genus erwinia].antibacterial activity of 272 erwinia strains was studied. it was found that 182 or 66.9 per cent of the strains were capable of producing spontaneously antibacterial substances belonging to the class of bacteriocins; 125 bacteriocynogenic strains were divided into 25 groups on the basis of their antibacterial spectrum similarity; 57 bacteriocynogenic strains were not included into any of these groups because of their significant heterogenicity with respect to the feature studied. it was shown t ...1978677837
the role of nationwide nosocomial infection surveillance in detecting epidemic bacteremia due to contaminated intravenous fluids.since january, 1970, the center for disease control (cdc) has corridnated surveillance of nosocomial infections in a group of voluntarily cooperating hispitals in the united states. in 1970, this surveillance system failed to realize one of its major goals: detection of a nationwide epidemic of septicemia caused by contaminated intravenous products. however, retrospective review of infections reported to cdc revealed that the data received were sufficient for the outbreak to have been recognized ...1978707489
exposure to dust-borne bacteria in agriculture. i. environmental order to evaluate the exposure of agricultural workers to dust-borne bacteria, microbiological examinations of the air were performed in grain storing and processing plants and in animal houses. large concentrations of viable microorganisms, ranging from 129.2 to 1289.9 thousands/m3 of air, were found in the rooms contaminated with grain dust and much lower in those contaminated with flour dust (22.6 thousands/m3). bacteria predominated in the air of grain plants, whereas actinomycetes and fu ...1978708119
[hemolytic, plasmocoagulase and hyaluronidase activity of bacteria of the genus erwinia]. 1978732602
[numerical taxonomic studies on enterobacteria of surface-water (author's transl)].435 enterobacterial strains of surface-water were tested in 35 physiological characters and classified numerically by a single-linkage procedure. the relations of similarity are shown in a "vernetzungsdiagramm" (connected graph, fig. 1). tab. 1 specifies the characters of the taxa, which were formed graphically. the following facts are the result of this taxonomic classification: 1. 32% of the escherichia group are kcn-positive. in addition it was found that 60% of the e. coli isolated from the ...1978735569
distribution of ice nucleation-active bacteria on plants in nature.a replica plating method for rapid quantitation of ice nucleation-active (ina) bacteria was developed. leaf washings of plant samples from california, colorado, florida, louisiana, and wisconsin were tested for the presence of ina bacteria. of the 95 plant species sampled, 74 were found to harbor ina bacteria. only the conifers were, as a group, unlikely to harbor ina bacteria. all of the ina bacteria isolated resembled either pseudomonas syringae or erwinia herbicola. sufficient numbers of ina ...1978736541
proteins, phospholipid distribution and fluidity in membranes of the gram-negative bacterium erwinia carotovora [proceedings]. 1978744424
[characteristics of the conjugation transfer of the r plasmids in bacteria of the intestinal group to erwinia cells].the conjugal transfer of seven r factors with different genes of drug resistance from escherichia coli bacteria to twenty erwinia strains was studied. seven erwinia strains were able to accept r plasmids, however the frequency of r factors transfer from e. coli to erwinia was about 10(-6)-10(-4), and as a rule was higher when the cross was made at optimum temperature for the donor. the most of r- exconjugants showed higher degree of resistance to streptomycin and lower degrees of resistance to t ...1978744476
[l-asparaginase activity of microorganisms].l-asparaginase content was studied in 67 different microorganisms, bacterial strains of e. coli, pseudomonas, erwinia, proteus, alcaligenes, bacillus, bacteria. xanthomonas, and yeasts and fungi-saccharomyces, candida, aspergillus, epidermophyton, trichophyton. all the bacterial strains studied possessed l-asparaginase activity, which varied greatly depending on the representative (10-1200 iu/g dry weight of cells). yeasts and fungi displayed low l-asparaginase activity.1978747012
identification of erwinia sp., causing stalk rot of maize in egypt.the physiological characteristics, pathogenic propensities, and the sensitivity towards certain antibiotics of the causal bacterium of stalk rot of maize, isolated in egypt, were studied. different isolates of erwinia carotovora and e. atroseptica were included for comparative studies. the antibiotic sensitivity tests (including erythromycin) are of no value in differentiating between e. carotovora and e. atroseptica. the cultures of the causal bacterium of stalk rot of maize showed physiologica ...1978749412
comparative experimental evaluation of immunodepressive and toxic effects of l-asparaginase (nsc-109229) from escherichia coli and from erwinia carotovora. 1976769978
derivation and properties of f-prime factors in escherichia coli carrying nitrogen fixation genes from klebsiella pneumoniae.a his+ nif+ escherichia coli k12, hfr strain (unf43) was constructed by an intergeneric mating between a klebsiella pneumoniae donor strain (hf3) and a his-hfr e. coli strain (sbi824) which transfers his as an early marker. an f-prime nif plasmid, fn39, carrying genes which correspond to the e. coli chromosomal region, metg gnd his shia, but excluding purf and arod, was isolated from unf43. translocation of carbenicillin resistance genes from a p-type r-factor, r68, to fn39 increased the stabili ...1976772161
[antigenic properties of 1-asparaginases isolated from different species and strains of bacteria].immunological relation of l-aspariginase isolated from different strains of e. coli and ervinia carotovera was investigated by the method of specific neutralization of the asparaginase effect on asparagin in the presence of immune antiasparaginase serum and the method of microprecipitation in agar. it was found that there was immunological relation between asparaginases of different strains of the same microbial species. at the same time common antigens were absent in asparaginases synthesized b ...1976776078
the modification of tyrosyl residues in l-asparaginases and ribonuclease a with fremy's salt. implication of tyrosyl residues in the activity of l-asparaginases from e. coli b and erwinia carotovora. 1975776662
a radiometric method for the measurement of l-asparaginase at concentrations below 1 x 10(-5) i.u./ml: technique and application. 1976786281
a comparative study of the antitumor effectiveness of e. coli and erwinia asparaginases.the relative antineoplastic effectiveness of e. coli and erwinia asparaginases was tested against lymphoid leukemias earad-1 and l5178y/ca55. e. coli and erwinia asparaginases had similar clearance rates from plasma in mice, and at a dose of 250 iu/kg body weight both enzymes lowered plasma asparagine to undetectable levels. nevertheless, the dosage of erwinia asparaginase needed to cause similar prolongation of median survival time in leukemic mice was at least twice that of e. coli asparaginas ...1976788892
comparison of anaphylactic reactions to asparaginase derived from escherichia coli and from erwinia cultures.a retrospective study was undertaken comparing the frequency and severity of anaphylactic reactions to e. coli-derived and erwinia-derived asparaginase given intravenously on a weekly dosage schedule. both drugs were found to produce life-threatening hypersensivity reactions with the chance of reaction per dose administered being almost identical--8% for each dose administered. eleven of 31 patients (35%) experienced anaphylactic reactions, 9/27 (33%) with e. coli and 3/10 (30%) with erwinia asp ...1976791476
the "other" asparaginase. 1976794672
demonstration of cell division by septation in a variety of gram-negative rods.through use of an initial fixative employing a combination of crotonaldehyde and glutaraldehyde, septa were preserved in thin sections of dividing cells of strains of pseudomonas aeruginosa, salmonella typhimurium, shigella sonnei, and escherichia coli when grown at 30 c in a dilute basal medium. the same procedures, however, revealed only a constrictive division process in proteus vulgaris and erwinia sp. this adds to the evidence that septation, although difficult to demonstrate, is the proces ...1975803487
genetic transfer of pseudomonas aeruginosa r factors to plant pathogenic erwinia species.the r factors rp1, r68 and r91 were freely transmissible to and from pseudomonas aeruginosa, salmonella typhimurium, and various plant pathogenic erwinia spp. the antibiotic resistance spectrum of r+ erwinia recipients was similar to those of other bacteria harboring these r factors, but maximum resistance levels differed with each recipient. the sponstaneous elimination of these factors from the erwinia strains and the ability to transfer multiple antibiotic resistance suggest that these exist ...1975804467
microflora and invert sugars in juice from healthy tissue of stored sugarbeets.bacterial populations increased in juice of healthy tissue of sugarbeet roots stored at 5 c. average counts showed a sixfold increase after 150 days of storage. invert sugar levels increased over threefold in "american 4 hybrid a" and remained fairly constant in "mono-hy d-2." the former cultivar also had significantly higher bacterial colony counts than the latter before 90 days of storage. of 36 isolates identified, 16 were pseudomonas spp. including p. chlororaphis; 6 bacillus spp. including ...1975808167
[purity criteria for bdellovibrio bacteriovorus cultures].two-component cultures of bdellovibrio bacteriovorus, a bacterial parasite, are not always pure; sometimes they contain microbial forms, different from the host and parasite, which cannot be isolated by conventional techniques of inoculation on solid growth media. the only way to isolate them is to apply techniques used for the reversion of l-forms of bacteria. the isolated microorganisms have been identified. the criteria of purity were established for two-component cultures consisting of the h ...1975809647
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