| expression of the hut operons of salmonella typhimurium in klebsiella aerogenes and in escherichia coli. | the normal hut (histidine utilization) operons, as well as those with mutations affecting the regulation of their expression, of salmonella typhimurium were introduced on an f' episome into cells of s. typhimurium and klebsiella aerogenes whose chromosomal hut genes had been deleted and into cells of escherichia coli, whose chromosome does not carry hut genes. the episomal hut operons respond in a manner very similar to induction and catabolite repression in all three organisms. the small differ ... | 1975 | 362 |
| aerobacter aerogenes prl-r3 urease. purification and properties. | a method for the preparation of a 150-fold purified and homogenous a. aerogenes urease is reported. the enzyme exhibited two ph optima at ph 7.0 and 7.5 in triethanolamine and phosphate buffer, respectively. the affinity of the enzyme toward its substrate increased with the increase of ph. no effect of the ph was observed on the measured temperature coefficient (q10). there was no discontinuity in the arrhenius plots at ph 5.4 and 7.5 but an upward discontinuity at ph 6.15 and 8.7 with transitio ... | 1975 | 879 |
| purification and some properties of a novel maltohexaose-producing exo-amylase from aerobacter aerogenes. | maltohexaose producing amylase (ec 3.2.1.-) is the fourth known exo-amylase, the three previously known being glucoamylase, beta-amylase and pseudomonas stutzeri maltotetraose producing amylase. the enzyme after release from aerobacter aerogenes cells by 0.1% sodium lauryl sulfate extraction was purified by ammonium sulfate precipitation, deae-sephadex column chromatography and sephadex g-100 gel filtration to 80-fold of the original sodium lauryl sulfate extract activity, it gave a single band ... | 1975 | 1094 |
| regulation of synthesis of glutamine synthetase by adenylylated glutamine synthetase. | we have examined three mutants of klebsiella aerogenes whose genetic lesions (glnb, glnd, and glne) are in loci unlinked to the structural gene for glutamine sythetase (glna) and in which the control of both the level and state of adenylylation of glutamine synthetase is altered. each mutation alters a different component of the adenylylation system of glutamine synthetase l-glutamate:ammonia ligase (adp-forming), ec 6.3.1.2. inability of the cell to deadenylylate glutamine synthetase (glnb and ... | 1975 | 1744 |
| the existence of three types of acetohydroxy acid synthetase in an isoleucine-requiring mutant of aerobacter aerogenes. | the synthesis of the three types of acetolactate synthase (ec 4.1.3.18) which are responsible for the biosynthesis os isoleucine and valine, was observed in aerobacter aerogenes i-12, an isoleucine-requiring mutant, when grown on the four kinds of media. when the cells were grown on isoleucine-rich medium, acetolactate synthase sensitive to feedback inhibition and having an optimum ph at 8.0 was formed. by increasing the amount of potassium phosphate in the medium, the catabolite repression of t ... | 1976 | 5136 |
| partial purification and properties of enterobacter cloacae heat-stable enterotoxin. | cell free preparations of the whole-cell lysate and ultrafiltration (uf) fractions of broth cultures of a strain of enterobacter cloacae, isolated from a puerto rican with tropical sprue, were assayed for their ability to induce in vivo net water secretion in the rat jejunum. the whole-cell lysate and um-10 retentate of broth cultures were inactive. the um-2 retentate and filtrate were active at a concentration of 100 mug/ml or more; the toxigenic activity was entirely retained, and increased to ... | 1976 | 5376 |
| kinetic properties of serratia marcescens adenosine 5'-diphosphate glucose pyrophosphorylase. | the regulatory properties of partially purified adenosine 5'-diphosphate-(adp) glucose pyrophosphorylase from two serratia marcescens strains (atcc 274 and atcc 15365) have been studied. slight or negligible activation by fructose-p2, pyridoxal-phosphate, or reduced nicotinamide adenine dinucleotide phosphate (nadph) was observed. these compounds were previously shown to be potent activators of the adpglucose pyrophosphorylases from the enterics, salmonella typhimurium, enterobacter aerogenes, e ... | 1976 | 6432 |
| antibacterial activity of sisomicin in comparison with gentamicin. | the antibacterial activity of sisomicin -- a new aminoglycoside antibiotic -- as compared with gentamicin was tested on 521 bacterial strains of different species in a serial-dilution test. staphylococci, streptococci, e. coli, klebsialla-enterobacter, indole-psitive proteus strains, pseufomonads, salmonads, salmonellae, and serratia marcescens were inhibited to the extent of 100% at a maximun of 4.0 mug/ml. sisomicin showed a higher antibacterial activity against part of the bacterial species. ... | 1976 | 9099 |
| the effect of large body size on the temperature regulation of the komodo dragon, varanus komodoensis. | | 1976 | 9248 |
| microbiology of the female genital tract during pregnancy and parturition. | | 1976 | 10196 |
| in vitro study of netilmicin compared with other aminoglycosides. | netilmicin (sch 20569) is an ethyl derivative of gentamicin c(1a) that is active against most enterobacteriaceae, pseudomonas aeruginosa, and staphylococcus aureus isolates. among 342 clinical isolates tested, all staphylococci; 92% of escherichia coli, 93% of klebsiella pneumoniae, and 92% of enterobacter were inhibited by 0.8 mug or less of netilmicin per ml, but only 78% of p. aeruginosa were inhibited by 3.1 mug or less per ml. most clinical isolates of enterococci, serratia marcescens, and ... | 1976 | 10829 |
| approach to a practical method for screening and identifying microorganism genera from urine (author's transl). | in this study the author reported upon a practical new system for screening and identifying the microbial agents causing urinary tract infections. this system is composed of a combination of 3 screening procedures (ph-value + nitrite-test + catalase-test) and 8 selective culture media for the purpose of genus identification within 24 hours (uripret-g). a total of 130 cultures was investigated. the employed microorganisms were mainly recovered from urine samples. they included the following speci ... | 1976 | 11179 |
| energetic aspects of anaerobic growth of aerobacter aerogenes in complex medium. | molar growth yields for anaerobic growth of aerobacter aerogenes in complex medium were much higher than for growth in minimal medium. in batch cultures the molar growth yield for glucose varied from 44 to 50 and yatp from 17.1 to 18.8. for glucose-limited chemostat cultures a value of 17.5 g/mole was found for y max atp and a value of 2.3 mmoles atp/g dry weight h for the maintenance coeficient. growth-dependent ph changes were used to control the addition of fresh medium, containing excess of ... | 1976 | 13757 |
| azlocillin: in vitro studies of a new semisynthetic penicillin. | the activity of azlocillin, a new semisynthetic penicillin, was determined against 582 clinical isolates of gram-negative bacilli and gram-positive cocci. over 75% of the isolates of pseudomonas aeruginosa were inhibited at a concentration of 12.5 mug or less per ml. azlocillin is also active against indole-negative and -positive proteus spp., inhibiting 98 and 71%, respectively, at a concentration of 12.5 mug or less per ml. isolates of klebsiella spp. and enterobacter spp. showed less suscepti ... | 1977 | 18083 |
| contamination of rat urine with gut flora using all-glass metabolism cages for collection of urine and faeces. | in rat urine collected in all-glass metabolism cages, at least four strains of intestinal microflora were found: two types of e. coli, enterobacter cloaceae and proteus vulgaris. the number of bacteria of each strain increased with time. 2. the ph of the urine increased from 6-9 after 24 h to 8-95 after 120 h. the ph of the urine of neomycin-treated rats remained nearly constant over a period of two days. 3. nitroreductase activity was present in the rat urine. added p-nitrobenzoic acid was redu ... | 1977 | 18846 |
| a new restriction enzyme from enterobacter cloacae (ecli). | | 1977 | 19297 |
| the development of aerobic spoilage flora on meat stored at chill temperatures. | | 1977 | 22531 |
| bacterial 2,3-butanediol dehydrogenases. | enterobacter aerogenes, aeromonas hydrophila, serratia marcescens and staphylococcus aureus possessing l(+)-butanediol dehydrogenase produced mainly meso-butanediol and small amounts of optically active butanediol; acetobacter suboxydans, bacillus polymyxa and erwinia carotovora containing d(-)-butanediol dehydrogenase produced more optically active butanediol than meso-butanediol. resting and growing cells of these organisms oxidezed only one enantiomer of racemic butanediol. the d(-)-butanedio ... | 1978 | 25056 |
| effect of ph on the antimicrobial activity of some triphenylmethane dyes. | four common dyes were tested as inhibitors of four types of bacteria over the ph range 5.0-9.0. inhibition of the gram-negative types, salmonella anatum and enterobacter aerogenes, was markedly affected by the ph of the medium. these organisms tolerated concentrations of crystal violet and ethyl violet about 100-fold higher at ph 5.0 than at ph 9.0. above ph 7.0 brilliant green (bg) and malachite green (mg) were precipitated as their respective carbinols and lost their inhibitory properties with ... | 1978 | 27297 |
| 1-n hapa gentamicin b, a new aminoglycoside active against gentamicin resistant isolates--activity compared to other aminoglycosides. | 1-n hapa gentamicin b is a new aminoglycoside active against most enterobacteriaceae, pseudomonas aeruginosa and staphylococcus aureus. among 504 clinical isolates at a concentration of 12.5 microgram/ml all staph. aureus, escherichia coli, klebsiella, enterobacter, proteus rettgeri, providencia and 78% of pseudomonas, 86% of proteus morganii were inhibited. like other aminoglycosides, the activity was greatest at an alkaline ph and reduced by high cations concentrations. 1-n hapa gentamicin b w ... | 1978 | 27494 |
| regulation of the assimilation of nitrogen compounds. | | 1978 | 28074 |
| piperacillin: in vitro evaluation. | the in vitro activity of a new semisynthetic penicillin, piperacillin, was determined against 577 clinical isolates of gram-positive cocci and gram-negative bacilli. a concentration of 12.5 mug/ml inhibited 92% of isolates of pseudomonas aeruginosa, 82% of serratia marcescens, 73% of escherichia coli, 61% of klebsiella spp., and 42% of enterobacter spp. most proteus spp. were extremely susceptible; over 85% were inhibited by 0.10 mug/ml. piperacillin failed to inhibit the growth of gram-negative ... | 1978 | 28694 |
| isolation of influenza virus from wild ducks (anas platyrhynchos). | in the course of studies on influenza virus ecology, influenzavirus a was isolated from a cloacal smear from a wild duck (anas platyrhynchos) caught in west slovakia. the strain was identified as a(hav7nav1). the results of virus isolation experiments from other species of aquatic and other small birds were negative. | 1978 | 29468 |
| studies of relationship among terrestrial pseudomonas, alcaligenes, and enterobacteria by an immunological comparison of glutamine synthetase. | antibody to purified glutamine synthetase from escherichia coli was prepared and used for an immunological comparison of glutamine synthetases from species of salmonella, citrobacter, enterobacter, serratia, proteus, erwinia, aeromonas, pseudomonas, acinetobacter, xanthomonas, alcaligenes, and paracoccus. the results of ouchterlony double diffusion experiments and quantitative microcomplement fixation studies indicated that the amino acid sequence of this enzyme was highly conserved in different ... | 1978 | 31146 |
| excretion in insects: function of gut and rectum in concentrating and diluting the urine. | the diverse excretory systems of insects exhibit several features that appear unusual when comparisons are made with the mammalian kidney. secretion by the malpighian tubules of a fluid that is unlike the blood in composition, substitutes for glomerular filtration. various reabsorptive functions, such as volume reduction, regulation of individual electrolytes, adjustment of osmotic concentration and ph regulation, which are associated with distinct renal segments in the mammalian kidney, all occ ... | 1977 | 20337 |
| rate of demethylation of methylmercuric chloride by enterobacter aerogenes and serratia marcescens. | | 1979 | 36188 |
| effect of environmental factors on inactivation of b12-dependent glycerol dehydratase from aerobacter aerogenes. | effect of temperature, ph and univalent cation on kinetics of self-activation of b12-dependent glycerol dehydratase (gd) from aerobacter aerogenes with co alpha-alpha-(5,6-dimethylbenzimidazolyl-co beta-adenosylcobamide (adocbl) was investigated. the activation energy of the process of gd inactivation is found to be 3.9 kkal/m, the effect of ph on gd inactivation being insignificant. monovalent cation is not required for the formation of gd-adocbl complex, but it protects the complex from selfin ... | 1976 | 11842 |
| ps-5, a new beta-lactam antibiotic. ii. antimicrobial activity. | ps-5, a new beta-lactam antibiotic, has relatively potent antimicrobial activity against gram-positive and gram-negative bacteria, especially the enterobacter groups, serratia marcescens, the proteus groups and klebsiella pneumoniae. the activity of ps-5 against many beta-lactamase-producing organisms is greater than that of cefoxitin or cefazolin. ps-5 has good therapeutic activity in mice infected with staphylococcus aureus smith or enterobacter cloacae 45. | 1979 | 38238 |
| rapid diagnosis of enterobacteriaceae. i. detection of bacterial glycosidases. | the paper describes a number of tests for the rapid detection of glycosidases including alpha-glucosidase, beta-glucosidase, beta-glucuronidase, beta-xylosidase and alpha-fucosidase. the methods use heavy suspensions of viable but non-multiplying bacteria in a buffered solution of a chromogenic substrate. the results of the tests are readable within 4 h. the application of these tests to a collection of 633 strains of enterobacteriaceae and vibrionaceae demonstrates that some of the tests may be ... | 1976 | 9774 |
| spore germination promoter of dictyostelium discoideum excreted by aerobacter aerogenes. | the nutrient medium in which aerobacter aerogenes was grown, contains a spore germination promoter (sgp) for the cellular slime mould dictyostelium discoideum. sgp can cuase synchronous spore germination in a short time, and triggers the germination process in just a few minutes. germination-promoting capacity of sgp decreases as it comes in contact with increasing number of spores. when spores activated by sgp are stored at 4 degrees c, they gradually return to the dormant state. sgp is compara ... | 1976 | 9416 |
| resistance of gram-negative bacteria to antibiotics in large calf agglomerations. | the antibiotic resistance of e. coli, citrobacter, enterobacter-klebsiella and pseudomonas aeruginosa strains isolated from calves was tested. a high proportion of multiresistance was found even in e. coli strains isolated from newborn calves. gram-negative bacteria isolated from animals in three large calfhouses were almost 100% resistant to ampicillin, tetracyclines and sulphonamides. multiresistance was general and varied from 5 to 12 antibiotics among different strains. initial high sensitiv ... | 1979 | 41425 |
| current resistence situation in a surgical and urological department. | spectrum and sensitivity of bacteria were studied at the surgical (534 positive wound smears) and the urological clinics (7879 urine specimens). krankenhaus nordwest, frankfurt/m., during the period of 1969-1971 and in 1973. the most common organisms identified in wound smears were e. coli, followed by staph. areus, aerobacter and proteus species. e. coli were also predominant in urine, but followed by enterococci, proteus and pseudomonas. e. coli, proteus species and especially pseudomonas incr ... | 1976 | 9344 |
| cloacal absorption of urine in birds. | | 1976 | 7422 |
| emergence of antibiotic resistance in hospitals, 1935-1975. | a limited review of the changes in susceptibility of common bacterial pathogens to available antibacterial agents is presented. significant developments in recent years include the following: (1) the emergence of streptococcus pneumoniae with decreased resistance to penicillin and of some strains resistant to several antibiotics; (2) a decline in prevalence of multi-drug-resistant staphylococcus aureus after 1960 following their increasing prevalence in the preceding years (these changes were me ... | 1979 | 45521 |
| observations on brilliant green agar with h2s indicator. | several formulations of brilliant green agar with an added h2s indicator were evaluated. results were optimum with variations of a basic formula consisting of 40 g of tryptic soy agar (difco), 8 g of lactose, 8 g of sucrose, 80 mg of phenol red, 1 g of sulfanilamide, 1.5 g of ferric ammonium citrate, 5 g of sodium thiosulfate pentahydrate, and 7 mg of brilliant green dye per liter. brilliant green dye was added after sterilization of the other components this formulation supported good growth of ... | 1976 | 7190 |
| regulation of enzyme formation in klebsiella aerogenes by episomal glutamine synthetase of escherichia coli. | we studied the physiology of cells of klebsiella aerogenes containing the structural gene for glutamine synthetase (glna) of escherichia coli on an episome. the e. coli glutamine synthetase functioned in cells of k. aerogenes in a manner similar to that of the k. aerogenes enzyme: it allowed the level of histidase to increase and that of glutamate dehydrogenase to decrease during nitrogen-limited growth. the phenotype of mutations in the glna site was restored to normal by the introduction of th ... | 1976 | 6431 |
| glutamate dehydrogenase: genetic mapping and isolation of regulatory mutants of klebsiella aerogenes. | the gene for glutamate dehydrogenase (gdhd) has been mapped in klebsiella aerogenes by p1 transduction. it is linked to pyrf and trp with the order pyrf-trp-gdh. complementation analysis using f' episomes from escherichia coli suggests an analogous location in e. coli. two mutants able to produce glutamate dehydrogenase in the presence of high levels of glutamine synthetase have been isolated. one, tightly linked to gdhd, shows normal repression control by glutamine synthetase but produces four ... | 1976 | 6429 |
| regulation of histidase synthesis in intergeneric hybrids of enteric bacteria. | regulation of the expression of the histidase coded by hutk of klebsiella aerogenes in salmonella typhimurium and in escherichia coli and of the expression of the histidase coded by huts of s. typhimurium in e. coli was investigated. the hutk histidase was found to be sensitive to catabolite repression in k. aerogenes and in e. coli, but insensitive to catabolite repression in s. typhimurium; huts histidase has previously been shown to be catabolite sensitive in all three organisms. the expressi ... | 1976 | 6426 |
| cephacetrile, a new cephalosporin: in vitro, pharmacological and clinical evaluation. | cephacetrile, a parenteral cephalosporin, was evaluated for in vitro antibacterial activity, clinical pharmacology and effectiveness in the treatment of severe infections. the antibacterial activity against 187 isolates was determined by an agar-dilution technique. the mics were 0.06 to 0.5 mug/ml for group a streptococcus, d. pneumoniae, and staph. aureus, 4-6 mug/ml for e. coli and klebsiella-enterobacter 8-32 mug/ml for pr. mirabilis and more than 500 mug/ml for ps. aeruginosa. a few strains ... | 1975 | 4377 |
| a polyvalent human gamma-globulin immune to pseudomonas aeruginosa: passive protection of mice against lethal infection. | as a means to development of guidelines for therapeutic application to human disease, preparations of human polyvalent gamma-globulin immune to pseudomonas aeruginosa (pg) were studied in acute infections in mice. pg was highly effective in controlling lethal infections induced in mice by the major immunotypes of p. aeruginosa; greater than or equal to 10 microgram of of gamma-globulin per mouse protected against challenge with less than or equal to 10(6) 50% lethal doses of p. aeruginosa. pg wa ... | 1977 | 70492 |
| transduction of chromosomal genes between enteric bacteria by bacteriophage p1. | we have used p1 transduction to create intergeneric hybrid strains of enteric bacteria by moving the gena and hut genes between klebsiella aerogenes, escherichia coli and salmonella typhimurium. the use of e. coli as the recipient in such transductions permits the construction of episomes and specialized transducing phage containing non-e. coli material. the effect of host restriction modification and deoxyribonucleic acid homology on the frequency of intergeneric transduction of these loci has ... | 1976 | 3494 |
| effect of intratumoral injection of bacterial and viral neuraminidase in rats. | we studied the effect of neuraminidase injection in rat's tumor at different doses: 5,10,50,100, 500 u and we concluded that: there was no difference between the rats treated with 5,10,50 u and the controls. the y died 3 weeks after the injection. but the rats treated by 100 at 500 u of na died quickley, in the week, of long metastases. | 1975 | 3291 |
| plasmid borne virulence of enterobacter hafniae. | the strain classified as enterobacter hafniae has been isolated in a severe epidemy of porcine diarrhoea. its pathogenicity has been found to be cotransmissible with resistance to oxytetracycline when the strain was grown in a mixed culture with some nonpathogenic e. hafniae strains as well as with escherichia coli. toxinogenity seems to be responsible for virulence of the strain. | 1978 | 76419 |
| enterobacter, b27, and ankylosing spondylitis. | | 1978 | 78003 |
| regulation of the hut operons of salmonella typhimurium and klebsiella aerogenes by the heterologous hut repressors. | in merodiploid strains of klebsiella aerogenes with chromosomal hut genes of k. aerogenes and episomal hut genes of salmonella typhimurium, the repressor of either species can regulate the hut operons of the other species. the repression exerted by the homologous repressor on the left-hand hut operon is, in both organisms, stronger than that exerted by the heterologous repressor. | 1975 | 363 |
| inactivation of citrate lyase from rhodopseudomonas gelatinosa by a specific deacetylase and inhibition of this inactivation by l-(+1-glutamate. | a previously unrecognized enzyme, citrate lyase deacetylase, has been purified about 140-fold from cell extracts of rhodopseudomonas gelatinosa. it catalyzed the conversion of enzymatically active acetyl-s-citrate lyase into the inactive hs-form and acetate. the enzyme exhibited an optimal rate of inactivation at ph 8.1. because of the instability of acetyl-s-citrate lyase at acidic and alkaline ph values, all assays were carried out at ph 7.2, where the spontaneous hydrolysis of the acetyl-s-ci ... | 1975 | 356 |
| modification of the ph-auxostat culture method for the mass cultivation of bacteria. | | 1978 | 26435 |
| a colorimetric method for the determination of indole, and its application to assay of tryptophanase. | | 1978 | 26285 |
| [simplified and accelerated methods of determining the generic affiliation of bacteria of the intestinal bacilli group]. | | 1979 | 86647 |
| response of fluorescamine during the interaction of cloacin df 13 with the isolated cytoplasmic membrane of susceptible cells. | | 1978 | 25773 |
| the immunotropic effect of cysteine in the foetal period and in adult rats after administration of thymus-independent antigens. | | 1977 | 25698 |
| identification of bacteroides fragilis by indirect immunofluorescence. | rabbit antiserum against live or heat-killed bacteroides fragilis nctc 9343 bacteria was titrated against hot phenol water-extracted polysaccharide antigens from five different species of the 'b. fragilis group' of bacteria using an enzyme immunoassay and shown to be specific for the b. fragilis nctc 9343 polysaccharide. when the antiserum was used in indirect immunofluorescence, 97.1% of 244 b. fragilis strains were correctly identified. only 8 of the other 312 bacteroides strains were stained ... | 1979 | 94651 |
| interaction of the complex between cloacin and its immunity protein and of cloacin with the outer and cytoplasmic membranes of sensitive cells. | | 1977 | 21081 |
| cefuroxime: a review of its antibacterial activity, pharmacological properties and therapeutic use. | cefuroxime is a new semisynthetic cephalosporin for parenteral administration. it is resistant to destruction by beta-lactamases produced by staphylococci and most gram-negative aerobic bacteria and is active against many bacteria resistant to cephalothin. cefuroxime is the most active of the cephalosporins against gonococci and haemophilus influenzae particularly against beta-lactamase producing strains. given by intramuscular or intravenous injection cefuroxime is effective against a wide vari ... | 1979 | 37064 |
| [disinfection of gutta-percha cones]. | | 1977 | 96557 |
| sk&f 75073, new parenteral broad-spectrum cephalosporin with high and prolonged serum levels. | sk&f 75073, a new parenteral cephalosporin, was found to have broad in vitro and in vivo antibacterial activity including isolates usually resistant to cephalothin and cefazolin. this activity included indole-positive proteus and enterobacter species and some serratia isolates. proteus mirabilis strains were particularly susceptible, as were haemophilus influenzae and neisseria species. the activity of sk&f 75073 against gram-positive bacteria was poorer than that of the control cephalosporins. ... | 1978 | 96734 |
| in vitro susceptibility of gentamicin and/or tobramycin resistant gram-negative bacilli to seven aminoglycosides. | the in vitro activity of gentamicin, tobramycin, sisomicin, netilmicin, amikacin, kanamycin and streptomycin was tested simultaneously by the agar dilution method against 584 clinical isolates of gram-negative bacilli that were resistant to gentamicin and/or tobramycin. about half of the gentamicin-resistant pseudomonas were susceptible to tobramycin but cross-resistance was virtually complete between gentamicin and tobramycin for enterobacteriaceae. sisomicin was much more active than gentamici ... | 1978 | 98451 |
| alkalimetric microassay of cephalosporins. | alkalimetric ph-stat titrations of cephalosporin c, cephacetril and their deacetyl derivatives using an acetyl esterase and beta-lactamase are described. the esterase was used to assay highly purified samples of cephalosporin c and cephacetril, and also to prepare analytically defined solutions of the deacetyl cephalosporins. lactamase-catalyzed hydrolysis of the parent compounds was then found to generate exactly 2 equivalents of acid per mole; that of the deacetyl derivatives exactly 1 equival ... | 1978 | 99413 |
| aperistaltic ureter in upper urinary tract infection--cause or effect? | | 1978 | 99855 |
| effect of metabolites on epsilon-n-hydroxylysine formation in cell-free extracts of aerobacter aerogenes 62-1. | the conversion of l-lysine to its corresponding epsilon-n-hydroxy derivative has been achieved for the first time by cell-free extracts of aerobacter aerogenes 62-1. partial fractionation by differential centrifugation (at 12 000 x g) revealed that both supernatant and pellet are essential for maximum enzymatic activity. the omega-n-hydroxylase (ec 1.14.99) was found to function optimally at ph 7-7.5 and exhibited an apparent km of about 75 mum for l-lysine. l(+)-lactate or dl-lactate and pyruva ... | 1977 | 18266 |
| on the mechanism of glutamine-dependent reductive amination of alpha-ketoglutarate catalyzed by glutamate synthase. | | 1977 | 16906 |
| [experiences with the uripret-system in pediatric practice (author's transl)]. | the present study reports on the application of the uripret-system for identification and susceptibility testing of microbial agents causing urinary tract infections carried out in a pediatric outpatient clinic. the findings were compared with those determined with the conventional methods in an institute of medical microbiology. for the detected mono- and mixed-cultures, in 85% of the cases a concurrent result of both methods was found. only in one case a different microbial agent genus was det ... | 1979 | 102911 |
| homology of the gene coding for outer membrane lipoprotein within various gram-negative bacteria. | the mrna for a major outer membrane lipoprotein from escherichia coli was found to hybridize specifically with one of the ecori and one of the hindiii restriction endonuclease-generated fragments of total dna from nine bacteria in the family enterobacteriaceae: e. coli, shigella dysenteriae, salmonella typhimurium, citrobacter freundii, klebsiella aerogenes, enterobacter aerogenes, edwardsiella tarda, serratia marcescens, and erwinia amylovora. however, among the enterobacteriaceae, dna from two ... | 1979 | 104972 |
| evidence for regulation of aerobacter aerogenes ph 6 acetolactate-forming enzyme by acetate ion. | | 1977 | 14639 |
| [our experience in the use of fosfomycin in human therapy]. | | 1978 | 105328 |
| immunophyletic relationships between actinomycetales and eubacteriales determined by examinations of their cytoplasmic antigens. | cytoplasms obtained from a total of 205 different strains of actinomycetales, eubacteriales, lower fungi, and protozoa were examined against selected anti-cytoplasm antisera using the immunodiffusion and counterimmunoelectrophoresis procedures. immunological relationships between the different genera have been revealed and, in respect to eubacteriales, the possible immunophyletic determinants have been discerned. cytoplasms of enterobacter, clostridium, staphylococcus, and diplococcus were found ... | 1975 | 46178 |
| purification and characterization of cloacin df13 receptor from enterobacter cloacae and its interaction with cloacin df13 in vitro. | extraction of the crude cell envelope fraction of cloacin df13-susceptible enterobacter cloacae strain 02 with triton x-100 and ethylenediaminetetraacetate solubilized an outer membrane fraction which neutralized the lethal activity of cloacin df13. a similar fraction could not be isolated from strains known to be lacking functional cloacin df13 receptors. on this basis the isolated outer membrane fraction was assumed to contain the specific cloacin df13 receptor. the receptor was purified to ho ... | 1979 | 35522 |
| [inorganic ion transport in bacteria]. | | 1979 | 107670 |
| influence of fatty acids on some microorganisms. | | 1979 | 108863 |
| hr 756, the syn isomer of a new methoxyimino cephalosporin with unusual antibacterial activity. | hr 756, the syn derivative of 7-[(2-(2-amino-4-thiazolyl)-2-methoxyimino)acetamido]cephalosporanic acid, is a new semisynthetic cephalosporin. it was 80 times more active than the anti derivative against beta-lactamase-producing strains of gram-negative bacteria. the range of inhibitory concentrations of hr 756 against gram-negative bacteria, including haemophilus influenzae, susceptible or resistant to penicillins and cephalosporins was from 0.01 to 0.1 mug/ml. this activity was consistently hi ... | 1978 | 31836 |
| study of a new strain of paramyxoviruses isolated from wild ducks: antigenic and biological properties. | among the different strains of avian paramyxoviruses isolated from migrating feral ducks, two were identified as newcastle disease viruses (ndv) and the five others would correspond to a new serotype for which we suggest the name of duck/mississippi/75 virus. the characteristics of this new serotype are as follows: (1) duck/mississippi/75 virus is able to grow as well in allantoic as in amniotic cavities of embryonated hen's eggs; (2) the haemagglutinin and haemolytic activities can be detected ... | 1979 | 90111 |
| mezlocillin: a new acyl ureidopenicillin. antimicrobial activity and combination effects with four aminoglycoside antibiotics. | mezlocillin (baypen), carbenicillin and mezlocillin combined with gentamicin, sisomicin, tobramycin and amikacin, respectively, were tested in vitro against 325 fresh clinical isolates of pathogenic gram-negative bacteria using the tube dilution procedure. the new acyl ureidopenicillin has a greater antimicrobial activity than carbenicillin against nearly all species tested. the comparison of the effectiveness of the mezlocillin-aminoglycoside combinations demonstrates the superiority of mezloci ... | 1978 | 95925 |
| the role of bacterial interference in the increased prevalence of oropharyngeal gram-negative bacilli among alcoholics and diabetics. | the oral flora of alcoholics, diabetics, and normal control subjects were compared using an agar overlay technique to determine whether the increased prevalence of oropharyngeal gram-negative bacilli among alcoholics and diabetics exists because patients with these diseases have decreased numbers of normal inhibitory bacteria in the oropharynx. alcoholics generally had slightly lower concentrations of inhibitory bacteria than control subjects, and diabetics had somewhat higher concentrations tha ... | 1979 | 39482 |
| nuclear division in the ameboflagellate adelphamoeba galeacystis. | nuclear division is synchronized cultures of the ameoboflagellate adelphamoeba galecystis has been described. division in this organism is typically promitotic. it occurs within an intact nuclear membrane and is characterized by the persistence of the nucleolus and its transformation into 2 polar masses. the nucleolus is stained with pyronin-y by the methyl green pyronin-y technic, and with heidenhain's hematoxylin, but is unstained by the feulgen reaction. the reaction with these stsins is remo ... | 1975 | 50441 |
| diminished effect of gentamicin under anaerobic or hypercapnic conditions. | the in-vitro activity of gentamicin, judged by m.i.c. determinations, was much reduced when a normal aerobic atmosphere was replaced either by air +4% co2 or by anaerobic conditions. the phenomenon was greatest for staphylococcus aureus, where a decrease in activity of up to 20-fold was found. for escherichia coli, klebsiella aerogenes, enterobacter spp., and proteus spp. the factor of decrease was between 15-fold and 2-5-fold. changes in medium ph, as a result of bacterial growth, can explain t ... | 1976 | 55718 |
| methods for studying the distribution of pigment in malpighian tubules of drosophila melanogaster. | the pigmented cells in malpighian tubules of drosophila melanogaster contain yellow globules which are blackened by osmium tetroxide. permanent preparations of the tubules showing pigmentation can thus be made. following prestaining acid hydrolysis, osmium-fixed tubules can be stained with orcein. for observations on pigment distribution and feulgen-dna content in the same preparation, tubules are fixed in mercuric chloride. color remains visible for several hours in unfixed tubules mounted in m ... | 1976 | 59419 |
| [detection among "e. aerogenes" strains of capsular antigens related to those of "klebsiella". interest of growth in metahydroxybenzoate to differenciate "e. aerogenes" and "k. pneumoniae" (author's transl)]. | forty-three strains of e. aerogenes isolated chiefly in morocco and france have been studied. thirty-five strains (81%) are surrounded with a thin capsule, antigenically related to klebsiella capsular antigens: k4 (2 strains), k4, 59 (1 strain), k11 (2 strains), k26 (7 strains), k42 (5 strains), k59 (3 strains), k68 (14 strains). one strain is capsulated but not typable with klebsiella capsular antisera. e. aerogenes and klebsiella capsular antigens are not identical but share common fractions y ... | 1977 | 72514 |
| amniotic fluid alpha-fetoprotein elevation with fetal omphalocele and a possible mechanism for its occurrence. | prenatal diagnosis of genetic disease and congenital malformations has become a major area of study in obstetrics. the assessment of amniotic fluid alpha-fetoprotein (afp) is useful for the diagnosis of neural tube defects. as more patients have been evaluated abnormal increases have been found in other defects, such as omphalocele, duodenal atresia, and congenital nephrosis. two patients with omphalocele are reported with afp measurement. a tenfold elevation of afp was found in the first patien ... | 1978 | 74953 |
| enterotoxigenicity of colonising coliform bacteria in tropical sprue and blind-loop syndrome. | the enterotoxigenicity of strains of klebsiella pneumoniae, enterobacter cloacae, and escherichia coli, which represented the predominant coliform species isolated from the jejunum of 12 patients with tropical sprue and 5 with the blind-loop syndrome, was quantitatively assessed in terms of the ability of toxin preparations to induce water secretion as assayed by in-vivo perfusion in the rat jejunum. all 12 patients with sprue harboured 1 or more highly toxigenic strains--14 of the 16 strains is ... | 1978 | 79710 |
| a further member of the yucaipa group isolated from the common wren (troglodytes troglodytes). | in 1977, 477 small birds of 52 species were virologically examined. five cloacal swabs from troglodytes troglodytes yielded two paramyxovirus strains which were found antigenically related to, but not identical with ch/yucaipa/california/56 virus according to three serological tests. the ecological significance of these findings and the possibility of paramyxovirus classification into four types are discussed. | 1979 | 94770 |
| polymicrobial bacteremia. | of 26,961 blood cultures taken during an 18-month period at the cincinnati general hospital, 1,715 (6%) were positive. ninety-four patients had blood cultures containing more than one organism. although aerobic and anaerobic streptococci were the most frequently isolated bacteria, a variety of microorganisms, including staphylococcus aureus and the klebsiella-enterobacter-serratia group, was isolated in different combinations depending on the underlying disease. neurological illness, malignant n ... | 1979 | 40048 |
| the clinical implications of contaminated pharmaceutical products. | | 1979 | 117898 |
| letter: enterobacter urinary infection. | | 1975 | 53578 |
| in vitro and in vivo laboratory studies on three hydroxyiminophenylacetyl cephalosporins with particular reference to sk&f 80303, an unusually long-acting cephalosporin. | three cephalosporins with 7-(2-hydroxyiminophenylacetamido) side chains (sk&f 79433, 80000 and 80303), differing in their 3-substituents, exhibited similar broad-spectrum antibacterial activity in vitro against strains of staphylococcus aureus, streptococcus faecalis and various gram-negative bacilli. all three were active in vivo (s.c., mouse) against s. aureus, escherichia coli or klebsiella pneumoniae, but they differed significantly in serum pharmacokinetic profiles. sk&f 80303 produced high ... | 1979 | 118958 |
| combined rifampicin and chloramphenicol therapy for enterobacter osteomyelitis. | a 2-month-old infant with enterobacter osteomyelitis complicating total parenteral nutrition was successfully treated with rifampicin and chloramphenicol. no untoward side effects attributed to rifampicin has been noted despite prolonged administration of rifampicin. | 1979 | 119955 |
| synthetic disaccharide-protein antigen for production of specific o2 antiserum for immunofluorescence diagnosis of salmonella. | antisera from rabbits immunized with the synthetic disaccharide paratose 1 leads to alpha 3 mannose, representive of salmonella o-antigen 2, covalently linked to bovine serum albumin (bsa), were used in indirect immunofluorescence studies for the identification of salmonella serogroup a (o-antigen 1,2,12) bacteria. among 1311 enteric bacteria tested, 497 were salmonella. the anti-paratose 1 leads to alpha 3 mannose-bsa serum identified correctly all the 63 serogroup a strains tested. no positive ... | 1978 | 78659 |
| quantitative assessment of bactericidal activities of beta-lactam antibiotics by agar plate method. | quantitative bactericidal activities of beta-lactam antibiotics were determined by the agar plate method. broth cultures, of which the colony-forming units were counted before the study, were inoculated on antibiotic-containing agar plates, utilizing a 10(-3), 10(-2), or 10(-1) dilution or undiluted culture plated with each 0.001-ml calibrated loop. these plates were incubated at 37 degrees c overnight, and the minimal drug concentration at which no bacterial growth was observed on the plates wa ... | 1978 | 102246 |
| feulgen-dna absorption spectrum of euchromatin and constitutive heterochromatin. | when the heterochromatin and euchromatin of triatoma infestans are maximally depurinated, their feulgen-dna spectral profiles exhibit a shoulder at about lambda = 530 nm. the shoulder is especially prominent in the curves of the heterochromatin. it is assumed that this pattern is related to richness in repetitive dna in the heterochromatin, favoring appearance of schiff reagent molecules di-substituted with adjacent apurinic acid aldehydes. | 1978 | 83338 |
| h2n2 influenza viruses in domestic ducks. | | 1979 | 84284 |
| on the mechanism of the chorismate mutase reaction. | | 1978 | 100134 |
| aminopeptidase i activities in several microorganisms. | aminopeptidase i activity which was found to be localized in the same subcellular fraction and to be similarly heat stable was partially purified by a common procedure from escherichia coli b, escherichia coli k12, enterobacter aerogenes, salmonella typhimurium, serratia marcescens pseudomonas aeruginosa, and proteus vulgaris. the enzyme preparations were shown to contain a single animopeptidase active toward both leucylleucine and methionylalanylserine by mixed-substrate initial-velocity kineti ... | 1978 | 110425 |
| amplification of plaque-forming cells in the spleen after intracloacal antigen stimulation in neonatal chicken. | neonatal chickens were primed by multiple or a single injection with sheep red blood cells (srbc) into the cloacal lumen and then were challenged by intravenous injection with the same antigen. direct plaque-forming cells (pfc) in the spleen were markedly increased shortly after intracloacal priming. this enhancing effect was antigen specific and was abolished by surgical bursectomy 24 h after intracloacal priming. a similar effect was also found in mature adult chickens, although the degree of ... | 1979 | 91577 |
| [antibacterial in-vitro activity of pipemidic acid and nalidixic acid (author's transl)]. | the in-vitro activity of nalidixic acid and pipemidic acid was investigated in a combined study in 450 freshly isolated bacterial strains using the agar dilution test. gram-positive cocci were resistant to both substances except for a few pipemidic acid sensitive staphylococci. both substances had good antibacterial activity in the gram-negative spectrum. however, the mic values of pipemidic acid were generally clearly lower than those of nalidixic acid. only pipemidic acid showed activity again ... | 1979 | 110574 |
| persistent cloaca in a cynomolgus monkey (macaca fascicularis). | a persistent cloaca was found to occur in a 15-year-old female cynomolgus monkey. this is the first case reported in a nonhuman primate. the embryological basis of this anomaly is discussed, and pertinent anatomical findings in this animal are reported which demonstrate the embryologic defect. | 1978 | 105140 |
| interpretive criteria for cefamandole and cephalothin disk diffusion susceptibility tests. | a multi-center study of 1,838 clinical isolates established the accuracy of diffusion susceptibility tests with 30-mug cephalothin disks and 30-mug cefamandole disks. the same interpretive zone standards can be applied to tests with either disk but the two drugs cannot be tested interchangeably. | 1979 | 106770 |
| isolation and characterization of influenza a viruses from wild ducks in northern japan: appearance of hsw1 antigens in the japanese duck population. | twenty-six influenza a viruses were isolated from cloacal and tracheal samples of 235 resident and 396 migratory ducks in miyagi prefecture, japan, in 1977--78. of these, twelve were antigenically related to the avian-origin hsw1 virus, a/duck/alberta/35/76 (hsw1n1), but their neuraminidase antigens were characterized as nav2-3, nav4 or n2. these antigenic configuration have not previously been reported. in addition, one strain in which the neuraminidase antigen was identified as nav4, was demon ... | 1979 | 42296 |
| [new species of nematode, skrjabillanus schigini sp. n. (camallanata: skrjabillanidae), from the white amur]. | a description and differential analysis of the nematode skrjabillanus schigini sp. n. are given. the species was found in the serous membrane of the swim bladder and mesentery of the white amur from pond farms of the krasnodar territory. the new species differs from s. amuri from the same host in having four rather than five pairs of pedunculated papillae supporting the caudal alae; in the presence of one rather than two chitinous plate in the cloaca; by the parasitism in the swim bladder wall r ... | 1975 | 130606 |
| epidemic occurrence of neonatal necrotizing enterocolitis. | in case-control studies of three epidemics of neonatal necrotizing enterocolitis (nec) in three different high-risk nurseries in three states, no particular risk factor was associated with affected infants or their mothers. epidemic cases had higher birth weights and apgar scores and fewer perinatal difficulties than those previously reported for sporadic cases. seven infants fed primarily breast milk were not protected against disease. early antibiotic therapy was associated with a significantl ... | 1979 | 108997 |
| ultrastructural studies on the malpighian tubule of the pill millipede, glomeris marginata (villers). | ultrastructural studies on the malpighian tubules of glomeris marginata (villers) reveal considerable morphological differences between the upper, fluid secreting, segment, and the lower segment which is at present of unknown function. previous reports have shown that the upper tubule has a high permeability to compounds of high molecular weight. this may be accounted for by the fact that the epithelium shows very extensive intercellular spaces which are linked directly to junctions apparently s ... | 1977 | 141332 |
| [colo-pyloric anastomosis with pyloroplasty in esophagoplasty]. | a procedure is presented, allowing to include the stomach in the intestinal pathway in cases of oesophagoplasties, preventing at the same time the development of anastomotic peptic ulcers. a colo-pyloric anastomosis is performed with the aboral stump of the elevated colic loop. the colic end is anastomosed terminoterminally to the pylorus, in such a way that a 6--7 cm pylorotomy is performed. the pyloric function is suppressed and the evacuation of the stomach's contents into the duodenum is fac ... | 1977 | 143683 |