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bacterial community dynamics during start-up of a trickle-bed bioreactor degrading aromatic compounds.this study was performed with a laboratory-scale fixed-bed bioreactor degrading a mixture of aromatic compounds (solvesso100). the starter culture for the bioreactor was prepared in a fermentor with a wastewater sample of a care painting facility as the inoculum and solvesso100 as the sole carbon source. the bacterial community dynamics in the fermentor and the bioreactor were examined by a conventional isolation procedure and in situ hybridization with fluorescently labeled rrna-targeted oligon ...19989501433
a highly selective pcr protocol for detecting 16s rrna genes of the genus pseudomonas (sensu stricto) in environmental samples.pseudomonas species are plant, animal, and human pathogens; exhibit plant pathogen-suppressing properties useful in biological control; or express metabolic versatilities valued in biotechnology and bioremediation. specific detection of pseudomonas species in the environment may help us gain a more complete understanding of the ecological significance of these microorganisms. the objective of this study was to develop a pcr protocol for selective detection of pseudomonas (sensu stricto) in envir ...19989647828
diagnostics of neisseriaceae and moraxellaceae by ribosomal dna sequencing: ribosomal differentiation of medical microorganisms.fast and reliable identification of microbial isolates is a fundamental goal of clinical microbiology. however, in the case of some fastidious gram-negative bacterial species, classical phenotype identification based on either metabolic, enzymatic, or serological methods is difficult, time-consuming, and/or inadequate. 16s or 23s ribosomal dna (rdna) bacterial sequencing will most often result in accurate speciation of isolates. therefore, the objective of this study was to find a hypervariable ...200111230407
diversity and seasonal variability of beta-proteobacteria in biofilms of polluted rivers: analysis by temperature gradient gel electrophoresis and cloning.the beta-subgroup of the proteobacteria has been shown to be important in aquatic habitats and was investigated in depth here by molecular 16s rrna techniques in biofilms of the elbe river and its polluted tributary, the spittelwasser river. the bacterial 16s rrna genes were cloned from each site, screened for beta-proteobacterial clones and sequenced. river biofilm clones from both rivers grouped into 9 clusters (rbfs). rbfs 1, 2, and 3 fell into the recently described betai cluster of cosmopol ...200312902230
the bacterially produced metabolite violacein is associated with survival of amphibians infected with a lethal fungus.the disease chytridiomycosis, which is caused by the chytrid fungus batrachochytrium dendrobatidis, is associated with recent declines in amphibian populations. susceptibility to this disease varies among amphibian populations and species, and resistance appears to be attributable in part to the presence of antifungal microbial species associated with the skin of amphibians. the betaproteobacterium janthinobacterium lividum has been isolated from the skins of several amphibian species and produc ...200919717627
isolation and metabolic characteristics of previously uncultured members of the order aquificales in a subsurface gold mine.culture-dependent and -independent techniques were combined to characterize the physiological properties and the ecological impacts of culture-resistant phylotypes of thermophiles within the order aquificales from a subsurface hot aquifer of a japanese gold mine. thermophilic bacteria phylogenetically associated with previously uncultured phylotypes of aquificales were successfully isolated. 16s ribosomal dna clone analysis of the entire microbial dna assemblage and fluorescence in situ whole-ce ...200212039766
the diversity of bacteria isolated from antarctic freshwater reservoirs possessing the ability to produce polyhydroxyalkanoates.the diversity of polyhydroxyalkanoates-producing bacteria in freshwater reservoirs in the ecology glacier foreland, antarctica, was examined by a cultivation-dependent method. isolated strains were analyzed phylogenetically by 16s rrna gene sequencing, and classified as members of alpha-, beta-, or gammaproteobacteria classes. polymerase chain reaction was used to detect pha synthase genes. potential polyhydroxyalkanoates (phas) producers belonging mainly to pseudomonas sp., and janthinobacteriu ...201424939384
caenorhabditis elegans employs innate and learned aversion in response to bacterial toxic metabolites tambjamine and violacein.bacteriovorus eukaryotes such as nematodes are one of the major natural predators of bacteria. in their defense bacteria have evolved a number of strategies to avoid predation, including the production of deterrent or toxic metabolites, however little is known regarding the response of predators towards such bacterial defenses. here we use the nematode c. elegans as a model to study a predators' behavioral response towards two toxic bacterial metabolites, tambjamine yp1 and violacein. we found t ...201627384057
synergistic combination of violacein and azoles that leads to enhanced killing of major human pathogenic dermatophytic fungi trichophyton rubrum.superficial mycoses caused by dermatophytic fungi such as trichophyton rubrum represent the most common type of worldwide human infection affecting various keratinized tissues in our body such as the skin, hair, and nails, etc. the dermatophytic infection is a significant public health threat due to its persistent nature and high recurrence rates, and thus alternative treatments to cure this fungal infection are urgently required. the present study mainly focused on the synergistic activity of v ...201526322275
representative freshwater bacterioplankton isolated from crater lake, oregon.high-throughput culturing (htc) methods that rely on dilution to extinction in very-low-nutrient media were used to obtain bacterial isolates from crater lake, oregon. 16s rrna sequence determination and phylogenetic reconstruction were used to determine the potential ecological significance of isolated bacteria, both in crater lake and globally. fifty-five crater lake isolates yielded 16 different 16s rrna gene sequences. thirty of 55 (55%) crater lake isolates had 16s rrna gene sequences with ...200415528517
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