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phenylalanine hydroxylase from chromobacterium violaceum. purification and characterization.phenylalanine hydroxylase was purified approximately 3000-fold to apparent homogeneity with a 13% yield and crystalized from l-phenylalanine-induced cells of chromobacterium violaceum. the enzyme was shown to be composed of a single polypeptide chain with an estimated molecular weight of approximately 32,000. some of the physical properties of the enzyme include: a stokes radius of 26.0 a, a sedimentation coefficient of 2.71 s, a diffusion coefficient of 8.20 x 10(-7) cm2/s, a frictional ratio o ...197933985
immunological relationships of bacterial l-asparaginases.rabbit antisera against l-asparaginase preparations from escherichia coli, erwinia carotovora, citrobacter sp. and chromobacterium violaceum showed on immunoelectrophoresis that only the enzymes from e. coli and citrobacter are immunologically related. purified preparations had to be used to determine the immunological cross-reactions. immunoelectrophoresis at different ph values yielded the zero mobility points of the enzymes. the activity of the er. carotovora preparation was enhanced up to fo ...197654404
the respiratory system of chromobacterium violaceum grown under conditions of high and low cyanide evolution.the particulate fraction of disrupted chromobacterium violaceum grown under cyanide-evolving conditions was unable to oxidize ascorbate plus n,n,n',n'-tetra-methyl-p-phenylenediamine (tmpd), but oxidized nadh and succinate by a linear respiratory pathway which was very resistant to inhibition by cyanide. when the bacteria were grown under conditions where little cyanide evolution occurred, particulate fractions developed the ability to oxidize ascorbate-tmpd by a pathway highly sensitive to cyan ...1975172598
adsorption and selection of rhizobia with ion-exchange papers.ion exchange papers were used to study the adsorption of 32p-labelled rhizobia on defined surfaces. two strains of rhizobium japonicum and one each of r. leguminosarum and r. lupini were compared with escherichia coli and bacillus subtilis. the ratio of adsorption to strong and to weak acid papers/strong and weak basic papers was consistantly higher for all rhizobial strains compared to the other bacteria. the process of desorption by increasing the ion-concentration causes about 35% desorption ...1975242293
[biochemical tests and nutritional requirements of chromobacterium violaceum]. 1977279056
chromobacterium violaceum infections. report of a fatal case.a case of systemic chromobacterium violaceum infection, believed to be the first reported in a papua new guinean, is described. autopsy disclosed multiple liver abscesses and the diagnosis was made by recovery of the organism from one of the abscesses. aspects of management of this uncommon infection are discussed.1979299336
microbiology problem: chromobacterium violaceum infection. 1979386795
fatal infection by chromobacterium violaceum--clinical and bacteriological study. 1979457214
osteomyelitis associated with chromobacterium violaceum sepsis. a case report. 1979479248
discrimination of rhizobium japonicum, rhizobium lupini, rhizobium trifolii, rhizobium leguminosarum and of bacteroids by uptake of 2-ketoglutaric acid, glutamic acid and phosphate.rhizobium strains (one each of rh. japonicum, rh. lupini, rh. leguminosarum) take up 2-ketoglutaric acid in general much faster and from lower concentrations in the medium than strains of escherichia coli, bacillus subtilis and chromobacterium violaceum. a strain of enterobacter aerogenes, however, is more similar to some rhizobium strains. the same strains of rhizobium take up also phosphate much faster and from lower concentrations than the other bacteria tested. 4 strains of rh. lupini proved ...1976818969
chromobacterium violaceum infection in a nonhuman primate (macaca assamensis).chromobacteriosis caused by chromobacterium violaceum was diagnosed as an assam macaque, macaca assamensis, that died 4 days after receipt of the yerkes primate center, it was received from a primate facility in florida where it has been housed with a group of rhesus monkeys for 5 years. the animal died suddenly without showing any signs of clinical illness. necropsy findings included extensive hepatic necrosis with the formation of multiple large cavitary lesions. foci of necrosis were also fou ...1976824500
the chemical structure of the lipid a component of lipopolysaccharides from chromobacterium violaceum nctc 9694.the chemical structure of the lipid a component of lipopolysaccharides from chromobacterium violaceum nctc9694 was studied. sequential treatment of lipopolysaccharide with alkali, acid, sodium borohydride and hydrazine allowed the isolation of a reduced glucosamine disaccharide. according to methylation studies and enzymic analysis with beta-n-acetylglucosaminidase the d-glucosamine residues are beta(1 leads to 6) linked. the disaccharide carries two phosphate groups, one being linked glycosidic ...1977862618
pathogenicity of nonpigmented cultures of chromobacterium violaceum.nonpigmented cultures of chromobacterium violaceum have been found to be similar to pigmented cultures in their virulence for mice and the pathology of their infections. clinicians and microbiologists should be prepared to consider nonpigmented c. violaceum in their differential diagnoses of infections caused by gram-negative bacteria. the laboratorian who is not aware of this possibility is likely to erroneously identify nonpigmented strains of c. violaceum as members of closely associated gene ...1977874071
beta-lactamase activity in chromobacterium violaceum.a strain of chromobacterium violaceum isolated from a fatally infected patient was found to produce a beta-lactamase. when the organism was grown in drug-free medium, beta-lactamase activity was barely detectable, but when it was grown in the presence of penicillin g, a much larger amount of activity was produced. the beta lactamase was active primarily against cephalosporins; it was sensitive to inhibition by cloxacillin but resistant to p-chloromercuribenzoate. thus this enzyme closely resembl ...1976977998
metabolism of n-carbobenzoxyl-l-tryptophan by chromobacterium violaceum.chromobacterium violaceum (atcc 12472) metabolizes n-carbobenzoxyl-l-tryptophan into its 2', 3'-dehydro-derivative, and indole-3-propionic acid into indole-3-acrylic acid. the biotransformation accurs in resting cell incubations, and in growing cultures of the bacterium. tryptophan in fermentation media enhances the ability of chromobacterium violaceum to perform the conversion by an undetermined mechanism. the amino acid also prevents stored cultures from losing the ability to accomplish the ...19751125258
letter: carbohydrate fermentation, kia, and tsi agar reactions of chromobacterium violaceum. 19751163494
identification of chromobacterium violaceum: pigmented and non-pigmented strains.the classification and, therefore, identification of chromobacterium violaceum has been based upon its ability to produce a violet pigment. although the organism may yield non-pigmented variants when subcultured on artificial media, the isolation of non-pigmented strains from pathological tissues or from nature had not been reported. with a method established for the identification of c. violaceum regardless of violet pigmentation, non-pigmented strains were isolated from nature. the presence of ...19751236933
unusual chromobacterium violaceum: aerogenic strains.this is the first report of chromobacterium violaceum strains that are aerogenic. two pigmented strains of c. violaceum and their nonpigmented variants produced gas in glucose broth. kliger iron agar, and triple sugar iron agar. they were similar morphologically and biochemically to other anaerogenic strains studied previously.19761254704
formation of indole-3-carboxylic acid by chromobacterium violaceum.l-tryptophan is converted to indole-3-carboxylic acid by growing cultures and resting cell suspensions of chromobacterium violaceum19761262308
absolute configuration of 3-hydroxy fatty acids present in lipopolysaccharides from various bacterial groups.the absolute configuration of 3-hydroxy fatty acids has been studied, which are present in the lipopolysaccharides of the following bacteria: phodopseudomonas gelatinosa, rh. viridis, rhodospirillum tenue, chromobacterium violaceum, pseudomonas aeruginosa, bordetella pertussis, vibrio metchnikovii, vibrio cholerae, salmonella spp., escherichia coli, shigella flexneri, proteus mirabilis, yersinia enterocolitica and fusobacterium nucleatum. the 3-hydroxy acids were liberated by strong alkaline hyd ...19761278168
modulation of endotoxin-induced monokine release in human monocytes by lipid a partial structures that inhibit binding of 125i-lipopolysaccharide.we have previously shown that the synthetic tetraacyl precursor ia (compound 406, la-14-pp, or lipid iva) was not able to induce the production of tumor necrosis factor, interleukin-1, and interleukin-6 in human monocytes but strongly antagonized lipopolysaccharide (lps)-induced formation of these monokines. this inhibition was detectable at the level of mrna production. to achieve a better understanding of molecular basis of this inhibition, we investigated whether lipid a precursor ia (la-14-p ...19921280625
bacterial chemistry. vi. biological activities and cytotoxicity of 1,3-dihydro-2h-indol-2-one derivatives.the biosynthetic pigment from chromobacterium violaceum bb-78, 1,3-dihydro-2h-indol-2-one and its derivatives exhibit biological activities such as antimicrobial action, low hemolytic effects on red blood cells and in vitro trypanocide activity. a relatively high cytotoxicity on v-79 hamster fibroblast cells of the biosynthetic pigment was found, although with the methylol derivative the toxicity was almost eliminated. the methylol derivative exhibited similar toxicity as nifurtimox, a known, co ...19921341576
bismuth subsalicylate in the treatment of h2 blocker resistant duodenal ulcers: role of helicobacter pylori.fifty nine patients with helicobacter pylori positive duodenal ulcers that failed to heal after a six week course of treatment with h2 blockers were randomly assigned to one of the following three regimens: (i) bismuth subsalicylate, 600 mg three times daily (n = 19), (ii) ranitidine, 300 mg at night (n = 20), (iii) bismuth subsalicylate plus ranitidine (n = 20). cumulative ulcer healing rates after four and eight weeks respectively were as follows: bismuth subsalicylate 74% (14/19) and 95% (18/ ...19921347278
fatal chromobacterium violaceum infections associated with exposure to stagnant waters. 19921528650
chemical and cultural characterization of cdc group wo-1, a weakly oxidative gram-negative group of organisms isolated from clinical sources.ninety-six strains of weakly oxidative gram-negative rods isolated primarily from clinical specimens form a distinct group that has been designated centers for disease control (cdc) group wo-1 (wo stands for weak oxidizer). the phenotypic characteristics of cdc group wo-1 were most similar to those of comamonas acidovorans, pseudomonas mallei, and cdc pink coccoid group iii. the wo-1 group can be differentiated from c. acidovorans by the oxidation of glucose (often weak and sometimes delayed), m ...19921537895
x-ray absorption studies of the cu-dependent phenylalanine hydroxylase from chromobacterium violaceum. comparison of the copper coordination in oxidized and dithionite-reduced enzymes.the coordination chemistry of the cu sites of phenylalanine hydroxylase (pah) from chromobacterium violaceum has been studied by x-ray absorption spectroscopy (xas). the exafs of the cu(ii) form of the enzyme resembles that of other non-blue copper proteins such as plasma amine oxidases and dopamine-beta-hydroxylase and is characteristic of a mixed n/o coordination shell containing histidine ligation. detailed simulations of the raw exafs data have been carried out using full curved-wave restrai ...19921606153
chromobacterium violaceum septicaemia: the intensive care management of two cases.the first human infection with chromobacterium violaceum was recorded in 1927, but since then there have been about 33 cases reported worldwide, including two from australia. chr. violaceum occurs in the tropics and subtropics and is generally considered to be nonpathogenic, but infection can occur in patients who are immunosuppressed and it has a high mortality rate. this paper presents the intensive care management of two cases of chr. violaceum infection occurring in far north queensland. the ...19921609950
cloning and expression of chromobacterium violaceum phenylalanine hydroxylase in escherichia coli and comparison of amino acid sequence with mammalian aromatic amino acid hydroxylases.the complete amino acid sequence (296 amino acids) of chromobacterium violaceum phenylalanine hydroxylase (pah) was determined by nucleotide analysis of a dna clone isolated using both a synthetic oligonucleotide probe based on the nh2-terminal amino acid sequence and an antibody against this enzyme. the apal i fragment (approximately 1.9 kilobase pairs) containing the entire pah gene was subcloned in pbluescript ii and induced by isopropyl-beta-d-thiogalactopyranoside. in order to eliminate fus ...19911655752
[first isolation of chromobacterium violaceum strains, also achromogenic, in drinking water]. 19901715722
serodiagnosis of helicobacter pylori infection in childhood.sera from 100 children (ages, 6 to 16 years) presenting with upper gastrointestinal symptoms were examined for antibodies to helicobacter pylori by enzyme-linked immunosorbent assay (elisa) based on crude, loosely cell-associated antigens and a partially purified urease antigen preparation. all children underwent endoscopy, and 20 children were shown to have h. pylori infection by histology or direct culture. serum anti-h. pylori immunoglobulin g (igg) levels (crude antigen) were clearly raised ...19902279995
pneumonia and meningitis caused by a new nonfermentative unknown gram-negative bacterium.seven isolates of an unclassified bacterium resembling flavobacterium spp. were characterized by growth requirements, microscopic examination, biochemical characteristics, antimicrobial susceptibility tests, protein profile analysis, and serologic data. the unclassified isolates were differentiated from flavobacterium meningosepticum, flavobacterium odoratum, flavobacterium balustinum, flavobacterium strain iib, chromobacterium violaceum, aquaspirillum serpens, and pseudomonas spp. the bacterium ...19892504766
bacterial chemistry-iii: preliminary studies on trypanosomal activities of chromobacterium violaceum products.it has been found that the pigment-i from chromobacterium violaceum, 3-[1,2-dihydro5-(5-hydroxy-1h-indol-3-yl)-2-oxo-3h-pyrrol-3-yli diene] 1,3-dihydro-2h-indol-2-one, has trypanocide activity. the formylated derivatives, pigment-iii, immobilized 100% of the trypanosoma cruzi at a level of 46 microm after 48 h of interaction with a total growth inhibition in the same period. pigment-i exhibited low toxicity and a dna synthesis inhibition similar to that of nifurtimox, a known trypanocide compoun ...19892512826
bacterial ethylene synthesis from 2-oxo-4-thiobutyric acid and from methionine.the ability of selected bacterial cultures to synthesize ethylene during growth in nutrient broth supplemented with methionine or 2-oxo-4-methylthiobutyric acid (kmba) was examined. although most cultures transformed kmba into ethylene, only those of escherichia coli spao and chromobacterium violaceum were able to convert exogenously added methionine to ethylene. in chemically defined media, e. coli spao produced the highest amounts of ethylene from methionine and kmba. this capability was affec ...19892559143
temperature-dependent incorporation of 4-amino-l-arabinose in lipid a of distinct gram-negative bacteria.the presence and the relative amount of 4-amino-l-arabinose in lipopolysaccharides of members of the enterobacteriaceae family and in a single strain of chromobacterium violaceum has been studied with regard to growth-temperature dependent variations. changes in the presence and the amount of 4-amino-l-arabinose (4-aa) were observed in almost all cases, but the variations observed were not consistent among different species. while salmonella minnesota and proteus mirabilis showed higher levels o ...19892583477
effect of growth conditions on production of violacein by chromobacterium violaceum (bb-78 strain).1. chromobacterium violaceum (strain bb-78 isolated in brazil) produces violacein, a substance potentially useful in phototherapy and with antibiotic and trypanocide activity. culture conditions were optimized for the production of violacein and changes in nutrients, temperature and ph were correlated to cellular growth. 2. methionine was the only absolute requirement for growth. alanine, arginine, tryptophan and vitamin b12 stimulated growth and the microorganism utilized both the d and l forms ...19892620169
mechanistic studies on phenylalanine hydroxylase from chromobacterium violaceum. evidence for the formation of an enzyme-oxygen complex.steady-state kinetic analysis of pterin-dependent phenylalanine hydroxylase from chromobacterium violaceum indicated that the enzyme follows a partially ordered reaction mechanism. the data suggested that oxygen is the first substrate to bind to the enzyme. this result was further supported by rapid-quench experiments in which the enzyme-oxygen complex was trapped to yield product. additional support for the presence of an enzyme-oxygen complex was derived from magnetic susceptibility measuremen ...19892719947
near-fatal septicaemia with chromobacterium violaceum.chromobacterium violaceum is a gram-negative organism which normally inhabits water and soil. human infection is unusual and is associated with a high mortality rate. we describe a typical case of disseminated infection with chr. violaceum in a male carpet cleaner. the possible origin and treatment of the infection is discussed.19892733620
an acute pleuropneumonia in a pig caused by chromobacterium violaceum.a 2.5-month-old, 30 kg duroc pig died 10 days after showing clinical signs of dyspnoea and diarrhoea. acute necrotizing and fibrinous pleuropneumonia with locally extensive lesions was found. chromobacterium violaceum was isolated from pneumonic lung tissues and intratracheal inoculation of a pure culture into two spf pigs reproduced lesions similar to those found in the natural infection.19892760278
adduct formation between the cupric site of phenylalanine hydroxylase from chromobacterium violaceum and 6,7-dimethyltetrahydropterin.the interaction of pterin-dependent phenylalanine hydroxylase from chromobacterium violaceum with the cofactor analogue 5-deaza-6-methyltetrahydropterin and the cofactor 6,7-dimethyltetrahydropterin (dmph4) has been investigated by multifrequency electron spin resonance (esr) spectroscopy. 5-deaza-6-methyltetrahydropterin, which lacks the n-5 nitrogen present in the pyrazine ring of dmph4, binds tightly to the cupric form of the enzyme; however, no changes are observed in the esr parameters of t ...19872822093
a comparative study of virulent and avirulent strains of chromobacterium violaceum.a clinical isolate and a soil isolate of chromobacterium violaceum were compared to determine differences in virulence-related characteristics. purified lipopolysaccharide (endotoxin) from the virulent, clinical strain was more reactive than that from the avirulent soil strain as determined by the limulus amebocyte lysate assay. there were no differences in hemolysin or cyanide production between the two strains. the virulent strain was more resistant to phagocytosis and intracellular killing by ...19882843271
phenylalanine 4-monooxygenase from chromobacterium violaceum. 19872885717
phenylalanine hydroxylase from chromobacterium violaceum is a copper-containing monooxygenase. kinetics of the reductive activation of the enzyme.pterin-dependent phenylalanine hydroxylase from chromobacterium violaceum contains a stoichiometric amount of copper (cu2+, 1 mol/mol of enzyme). electron paramagnetic resonance spectroscopy of the enzyme indicates that it is a type ii copper-containing protein. the oxidized enzyme must be reduced by a single electron to be catalytically active. dithiothreitol was found to be an effective reducing agent for the enzyme. electron paramagnetic resonance data and kinetic results indicate the formati ...19863024714
structural heterogeneity regarding local shwartzman activity of lipid a.the relation of chemical structure to local shwartzman activity of lipid a preparations purified by thin-layer chromatography from five bacterial strains was examined. two lipid a fractions from e. coli f515--ec-a2 and ec-a3--exhibited strong activity, similar to that of previous synthetic e. coli-type lipid a (compound 506 or la-15-pp). the ec-a3 fraction contained a component that appeared to be structurally identical to compound 506, and the main component of ec-a2 fraction was structurally s ...19883057331
comparison of the in vitro activity of ciprofloxacin and 24 other antimicrobial agents against clinical strains of chromobacterium violaceum.eleven clinical strains of chromobacterium violaceum were tested for their susceptibility to 25 antimicrobial agents. ciprofloxacin was the most active of the compounds tested although norfloxacin and pefloxacin were highly active. no resistance was detected to mezlocillin, piperacillin, apalcillin, imipenem, and aztreonam while a single strain was resistant to ticarcillin. among the cephalosporin/cephamycin group only cefotetan showed good in vitro activity. gentamicin was more active than amik ...19883168426
[chromobacterium violaceum infection. presentation of a case with fatal outcome]. 19863299554
aerocavin, a new antibiotic produced by chromobacterium violaceum.a new antibiotic, aerocavin, has been isolated from fermentation broths of a non-pigmented strain of chromobacterium violaceum. the structure 1 was deduced from its spectroscopic properties and x-ray diffraction analysis. aerocavin exhibits activity in vitro against gram-positive and gram-negative bacteria.19883372350
aerocyanidin, a new antibiotic produced by chromobacterium violaceum.aerocyanidin, a new antibiotic containing an isonitrile group, has been isolated from fermentations of chromobacterium violaceum atcc 53434. structure 1 was assigned on the bais of spectroscopic characterization of the antibiotic and of a degradation product that results from treatment with base. the antibiotic is primarily active against gram-positive bacteria.19883372351
chromobacterium violaceum meningitis--a case report. 19873449453
chromobacterium violaceum phenylalanine 4-monooxygenase. 19873600378
glucose-6-phosphate dehydrogenase deficiency, neutrophil dysfunction and chromobacterium violaceum sepsis. 19873681551
cell-free biosynthesis of arphamenine a.arphamenine a was synthesized in a cell-free system obtained from the arphamenine-producing strain, chromobacterium violaceum bmg361-cf4. l-[14c]-phenylalanine was converted to beta-phenylpyruvic acid by phenylalanine amino-transferase obtained from the 10,000 x g supernatant (s10 fraction). [14c]-benzylmalic acid was synthesized from beta-phenylpyruvic acid with [14c]-acetyl-coa in the s10 fraction. [14c]-benzylsuccinic acid was formed from beta-phenylpyruvic acid with [14c]-acetyl-coa and atp ...19863707594
first case report from argentina of fatal septicemia caused by chromobacterium violaceum.a case of chromobacterium violaceum fatal septicemia is reported. the microorganism was isolated from six blood cultures and two suppurated skin lesions. it is the first such case described in argentina, and it reinforces the need for prolonged treatment and careful clinical evaluation to ensure complete remission of human infections caused by this bacterium.19863711283
nonfatal chromobacterial sepsis.we have described a case of multiple liver abscesses caused by chromobacterium violaceum. this organism should be considered in any case of unexplained liver abscess occurring in the southeastern united states. successful treatment of liver abscesses caused by this organism may be accomplished with appropriate antibiotic therapy.19863750002
[fatal septicemia due to chromobacterium violaceum].a 2-year old boy was suffered from an infection caused by chromobacterium violaceum. this is the first case of c. violaceum infection reported in taiwan. the boy was treated with chloramphenicol and gentamicin but died after one month of illness due to the incooperation of patient's family. the causative agent was isolated from patient's blood and scar. complete bacteriologic characteristics and antimicrobial susceptibility pattern of this isolate are reported.19863816371
novel potentiators of beta-lactam antibiotics. isolation of sq28,504 and sq28,546 from chromobacterium violaceum.two novel compounds, sq28,504 and sq28,546 are produced by chromobacterium violaceum. these compounds enhance the antibacterial activity of beta-lactam antibiotics against gram-negative organisms. both sq28,504 and sq28,546 induce morphological changes in the presence of beta-lactam antibiotics. only sq28,546 has weak antimicrobial activity against several gram-negative organisms.19853839229
chromobacterium violaceum adenitis acquired in the northern united states as a complication of chronic granulomatous disease. 19854080593
organization of bacteriophage tail-like particles in cells of chromobacterium violaceum.a strain of chromobacterium violaceum has been isolated which produces bacteriophage tail-like particles in high numbers. the extracellular morphology and the intracellular arrangement of these particles are described.19724112258
the intracellular organization of bacteriophage tail-like particles in cells of chromobacterium violaceum following mitomycin c treatment. 19734122865
the tryptophan hydroxylase of chromobacterium violaceum. 19744279915
biosynthesis of cyanide from [2-14c-15n]glycine in chromobacterium violaceum. 19694309602
successful treatment of systemic chromobacterium violaceum infection. 19744479291
purification and characterization from chromobacterium violaceum of an enzyme catalyzing the synthesis of gamma-cyano-alpha-aminobutyric acid and thiocyanate. 19734760496
mesosome structure in chromobacterium violaceum.exponentially growing cells of the gram-negative bacterium chromobacterium violaceum demonstrate invaginations of the cytoplasmic membrane with a high frequency. these invaginations conform to the ultrastructural appearance of mesosomes of gram-positive bacteria. as many as four mesosomes are observed per cell, each of which may increase the total membrane surface of the cell by 30%. washing of cells in dilute tris(hydroxymethyl)aminomethane buffer effects a distension of the mesosome "neck" and ...19744828310
fatal chromobacterium violaceum septicemia. 19715094501
chromobacterium violaceum infection in pigs. 19695387602
natural infections of gibbons with a bacterium producing violet pigment (chromobacterium violaceum). 19695388196
gamma-cyano-alpha-aminobutyric acid. a new product of cyanide fixation in chromobacterium violaceum. 19705417261
fatal infection due to chromobacterium violaceum in vietnam. 19705471231
chromobacterium violaceum: pathogenic for man. 19715544148
fatal septicemia due to chromobacterium janthinum. 19685661183
tryptophan synthetic pathway and its regulation in chromobacterium violaceum.extracts of chromobacterium violaceum catalyzed all of the reactions involved in synthesizing tryptophan from chorismic acid. tryptophan auxotrophs which had lost any of these activities did not produce the characteristic purple pigment, violacein, when grown on a medium in which tryptophan was limiting. gel filtration of extracts allowed us to estimate molecular weights for the tryptophan enzymes. all of the enzymes appeared to have molecular weights below 100,000. no enzymes were observed to o ...19685669904
fatal infection due to chromobacterium violaceum. 19685698884
beta-cyanoalanine formation by chromobacterium violaceum.nonproliferating cells of chromobacterium violaceum incubated with glycine, methionine, and succinate as substrates accumulated beta-cyanoalanine in the culture fluid. tracer experiments showed that carbons-2, -3, and -4 of beta-cyanoalanine are derived from the 2-carbon of glycine. when methionine-methyl-(14)c, succinate-1,4-(14)c, or succinate-2,3-(14)c was used as substrate, beta-cyanoalanine did not become labeled. if k(14)cn and serine were used as substrates, the cyano group of beta-cyanoa ...19695764335
extracellular enzymes produced by a pseudomonas sp. and their effect on cell envelopes of chromobacterium violaceum. 19695773756
effect of cold temperatures on the viability of chromobacterium violaceum.the effect of low, nonfreezing temperatures on the viability of five strains of chromobacterium violaceum was studied. the viability of cultures grown at 30 c was determined after exposure to various diluents held at 0 to 2 c. a culture diluted at its growth temperature served as the control. cells of strain n were most sensitive in the early part of the exponential phase of growth. cells of strains 252 and 341 were most sensitive in the late exponential, early stationary phase of growth. cells ...19695774755
effect of enzymes on the composition and structure of chromobacterium violaceum cell envelopes.cell envelopes of chromobacterium violaceum were isolated and treated under controlled conditions with trypsin, pronase, lipase, phospholipase c, lysozyme, and a mixture of enzymes produced by a bacteriolytic pseudomonas sp. after each enzyme treatment, losses in dry weight, protein, lipid, carbohydrate, 2,6-diaminopimelic acid, and total phosphorus were determined. electron-microscopic examination of the enzyme-treated envelopes indicated complete or partial loss of envelope rigidity or some en ...19695776532
cyanide formation from glycine by nonproliferating cells of chromobacterium violaceum. 19655851865
the isolation and characterization of glucosamine and muramic acid from the cell-wall mucopeptide of the gram-negative bacterium, chromobacterium violaceum. 19665956935
properties of surface materials released from cells of chromobacterium violaceum. 19665966705
chromobacterium violaceum septicaemia in a dog. 19836314965
[effect of solid substrates of microbial colony development].bacteria have been found to grow differently at contact sites to both organic and anorganic solids as on pure agar media. of 16 ad hoc selected bacterial strains, the following species (arranged in decreasing order) responded with stronger colony formation: bacillus subtilis, b. cereus, streptococcus sanguis, s. saprophyticus, s. faecalis, escherichia coli, micrococcus flavus, actinomyces viscosus, veillonella sp., enterobacter liquefaciens, yersinia enterocolitica. lactobacillus casei behaved n ...19846326398
[bacteria and dietary fiber].the presence of cellulose particles, so in-vitro studies have shown, influences the growth rate and metabolic activity of the bacteria escherichia coli, bifidobacterium adolescentis and chromobacterium violaceum. after the homogenization in waring blendor, the growth rate has been found to be highest in a medium containing 10% cellulose particles and to show a decreasing tendency in media containing 5%, 1% and 0% cellulose. the biochemical intensity, judged by glucose consumption and intensity o ...19846326399
recurrent infection with chromobacterium violaceum: first case report from south america.a case of chromobacteriosis in a young brazilian with toxaemia and multiple skin abscesses is described. the infection responded to treatment with chloramphenicol and cotrimoxazole but recurred 18 months later following insect bites received while fishing in a river. chromobacterium violaceum was subsequently isolated from the river water. this is the first case of this kind to be reported from south america.19846334119
nature and location of amide-bound (r)-3-acyloxyacyl groups in lipid a of lipopolysaccharides from various gram-negative bacteria.it has previously been demonstrated [eur. j. biochem. 124, 191-198 (1982) and 137, 15-22 (1983)] that the lipid a component of salmonella and proteus lipopolysaccharides contains amide-linked (r)-3-acyloxyacyl residues. in the present study lipid a of other gram-negative bacteria was analysed for the presence of amide-bound 3-acyloxyacyl residues. it was found that such residues are constituents of all lipid a tested (agrobacterium tumefaciens, chromobacterium violaceum, pseudomonas aeruginosa, ...19846437812
chromobacterium violaceum as a cause of periorbital cellulitis. 19846514595
hepatic drug-metabolizing enzyme system and endotoxin tolerance: structural requirement of lps in induction of an early tolerance.the alteration of hepatic drug-metabolizing enzyme activities in mice given salmonella endotoxin by single or multiple intraperitoneal injections was investigated. an essentially the same biphasic, early and late phase, endotoxin tolerance was observed in the animals receiving a single injection of endotoxin or repetitive daily injections. the results of reciprocal cross tolerance tests using lipopolysaccharide and free lipid a preparations derived from salmonella minnesota, salmonella typhimuri ...19846521669
human infection due to chromobacterium violaceum: a report from ilorin, nigeria. 19846535708
chromobacterium violaceum infection of the eye. a report of two cases.we report what we believe to be the first two known cases of chromobacterium violaceum infection of the eye. one patient had conjunctivitis as the initial manifestation of an ultimately fatal infection. in the second patient, who suffered from chronic granulomatous disease of childhood, orbital cellulitis developed as part of a disseminated c violaceum infection from which he subsequently recovered. infections caused by c violaceum may involve the eye and orbit. these infections are frequently f ...19846721759
the effect of some varying lipid a structures on the inhibition of fibrillogenesis in basement membrane collagen.acid soluble basement membrane collagen (abmc) was prepared by extraction of the anterior lens capsules from bovine calf eyes in 0.5 m acetic acid in the presence of the protease inhibitors leupeptin and pepstatin. thermal aggregates formed from soluble basement membrane collagen were facilitated by heating (28 degree c) the collagen solutions in 0.15 m phosphate buffer. the effects of endotoxins derived from salmonella minnesota r595, chromobacterium violaceum an rhodopseudomonas viridis on the ...19826801242
biosynthesis of monobactam compounds: origin of the carbon atoms in the beta-lactam ring.the biosynthesis of monobactams by strains of chromobacterium violaceum, acetobacter sp., and agrobacterium radiobacter was studied. monobactams were produced during logarithmic growth by c. violaceum and acetobacter sp. and during late log growth on glycerol and in stationary phase by a. radiobacter. the addition of various amino acids failed to significantly stimulate monobactam production in any of the producing organisms. several 14c-amino acids and pyruvate were incorporated in vivo into mo ...19826805424
chromobacterium violaceum infectious and chronic granulomatous disease. 19836824267
effect of bestatin analogues and other compounds on enkephalin hydrolysis by an aminopeptidase from the mesophiles pseudomonas sp atcc 11299a and chromobacterium violaceum atcc 12540.in our studies on newly synthesized compounds for their potential analgesic effect, we decided for purposes of convenience and economy to investigate non-mammalian sources for the presence of enkephalin degrading enzymes. an aminopeptidase that catalyzes the hydrolysis of the tyrosylglycyl bond of leucine- and methionine enkephalin was purified from the mesophiles pseudomonas sp atcc 11299a (ps) and chromobacterium violaceum atcc 12540 (cv). each preparation also hydrolyzed to varying extents ne ...19836856949
sulfur metabolism in the biosynthesis of monobactams.we studied the biosynthesis of monobactams with respect to sulfur metabolism in chromobacterium violaceum, acetobacter sp., and agrobacterium radiobacter. all three organisms used inorganic sulfur for monobactam production. when sulfur-containing amino acids were assayed as a source of sulfur for monobactam production, c. violaceum used cystine but not cysteine or methionine, acetobacter sp. used all three compounds, and a. radiobacter used none. 35s from cysteine, methionine, and sodium sulfate ...19836859838
a probable case of chromobacterium violaceum infection in australia.a case of a north-queensland aboriginal child, who died of a septicaemic illness probably caused by chromobacterium violaceum, is reported. this organism is a gram-negative bacillus found in soil and water. human and animal infections are very rare, but have been reported from various tropical and subtropical areas of the world. most of these have been fatal, although survival after recognition, appropriate antibiotic therapy, and surgical intervention has also been reported.19836865828
the laboratory diagnosis of opportunistic infections caused by uncommon bacteria in papua new guinea.flavobacterium meningosepticum, chromobacterium violaceum and listeria monocytogenes are opportunistic pathogens of man and are occasional but important causes of infections in neonates, infants and adults in papua new guinea. mortality is high. flavobacterium meningosepticum should be suspected when a weak indole producing oxidase positive gram-negative bacillus producing a discrete yellow non-diffusing pigment at room temperature and showing a resistant antibiotic sensitivity pattern is isolat ...19816951339
chromobacterium violaceum septicemia in new jersey. 19826953253
sq 26,180, a novel monobactam. i taxonomy, fermentation and biological properties.strains of chromobacterium violaceum, isolated from various environments, were found to produce a novel monocyclic beta-lactam antibiotic. the antibiotic, sq 26,180 was weakly active against gram-positive and gram-negative bacteria with the exception of mutants hypersensitive to beta-lactam antibiotics. the compound was highly stable to beta-lactamases and acted as an inhibitor of the p99 enzyme from enterobacter cloacae. sq 26,180 showed affinity for penicillin-binding proteins 1a, 4 and 5/6 of ...19826978877
chronic granulomatous disease of childhood and chromobacterium violaceum infections in the southeastern united states.patients with chronic granulomatous disease are predisposed to infections by catalase-positive organisms in the environment. chromobacterium violaceum is a catalase-positive bacterium whose saprophytic source in this country is the subtropical soil and water of the southeastern united states. two patients with chronic granulomatous disease, followed at the national institutes of health in maryland, acquired c. violaceum infections in florida. all 10 cases previously reported were acquired in flo ...19827092006
isolation of chromobacterium spp. from foods, soil, and water.chromobacterium violaceum, a soil and water inhabitant, has been implicated in human disease with a high mortality rate, particularly in the southeastern united states. the psychrotrophic chromobacterium lividum has been isolated from foods, water, and soil, but is not considered pathogenic. to determine the distribution of chromobacterium spp. in soil, water, and foods in the gainesville area, we evaluated bennett, ryalls and moss, and aeromonas membrane agars for their ability to recover these ...19827159087
attempts at induction of phenylalanine hydroxylase in thermophilic bacteria and induction of thermophily in mesophiles.some moderate and extreme thermophilic bacteria grew well on media other than the recommended basic media. attempts to induce phenylalanine hydroxylase (phe h) in the various thermophiles as well as mild thermophily in the mesophiles pseudomonas sp atcc 11299a and chromobacterium violaceum atcc 12540 were unsuccessful. evidence is presented indicating that the enzyme in the latter two organisms may be membrane bound. the level of phe h activity induced was not always consistent with the level of ...19817244367
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