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ecological studies on azotobacter in egyptian soils.the present survey includes 156 representative soil samples. results obtained confirm the richness of egyptian soils, particularly the nile valley soils, in azotobacter (60% of the samples contained greater than 10(3) colonies/g soil). colony counts were lower than mpn estimations. glucose is recommended for use in plating medium. among the environmental factors affecting azotobacter densities in soils of egypt are: organic carbon content, total soluble salt content, ph and type of the soil, dep ...197944932
biological nitrogen fixation in the terrestrial environment of a high arctic ecosystem (truelove lowland, devon island, n.w.t.).arranged in descending order of nitrogen-fixing (acetylene-reducing) potential the sites examined were mesic meadow and peat polygon troughs (equal rank), transition zone between mesic meadow and gravel ridge, gravel ridge, polar dessert, and peat polygon tops. the dominant nitrogen-fixing microorganisms, as in other arctic areas, were blue-green bacteria, especially those epiphytic on arctic mosses. the epiphytic association exhibited an optimum temperature for fixation of 20 degrees c. other b ...197896927
application of the fluorescent antibody technique to the study of an isolate of beijerinckia in soil.fluorescent antibody was prepared against a temperate-soil isolate of beijerinckia obtained from a rhizosphere of rice growing in camargue (france). the antibody did not cross-react with any of 6 species of azotobacter, 4 species of beijerinckia, or 44 unidentified soil bacteria isolated from a spectrum of rhizospheres, but strongly stained the homologous beijerinckia isolate. the isolate grew well in autoclave camargue soil, but increased in numbers only slightly in nonsterile soil during 9 day ...1977319880
microbiological examination of some sudanese soils.the composition of the microflora in different layers of four representative oil profiles of sudan were studied. counts of all types of microorganisms decreased significantly with depth in soil. azotobacter, in particular, occurred in high densities; representative strains were isolated and studied for their different characteristics. beijerinckia was detected as well, and a new method for the estimation of their numbers in pure cultures, based on the overlay agar technique, is described.1979398646
bacterial and fungal oxidation of dibenzofuran.cunninghamella elegans and a mutant strain (b8/36) of beijerinckia both oxidized dibenzofuran to 2,3-dihydroxy-2,3-dihydrodibenzofuran. the bacterial metabolite was extremely unstable and, in the presence of acid, was rapidly converted into a mixture of 2- and 3-hydroxydibenzofuran. in contrast, the 2,3-dihydroxy-2,3-dihydrodibenzofuran formed by c. elegans was stable and only yielded 2- and 3-hydroxydibenzofuran when heated under acidic conditions. the results suggest that beijerinckia b8/36 an ...1979486097
metabolism of dibenzothiophene by a beijerinckia species.beijerinckia b8/36 when grown with succinate in the presence of dibenzothiophene, accumulated (+)-cis-1,2-dihydroxy-1,2-dihydrodibenzothiophene and dibenzothiophene-5-oxide in the culture medium. each metabolite was isolated in crystalline form and characterized by a variety of chemical techniques, cis-naphthalene dihydrodiol dehydrogenase, isolated from pseudomonas putida, oxidized (+)-cis-1,2-dihydroxy-1,2-dihydrodibenzothiophene to a compound that was tentatively identified as 1,2-dihydroxydi ...1977596875
influence of partial pressures of acetylene and nitrogen upon nitrogenase activity of species of beijerinckia.acetylene reduction and nitrogen fixation by strains of beijerinckia indica and b. lacticogenes increased with increased partial pressures of acetylene and nitrogen up to 80 kpa. the optical emission spectrophotometric method was used for the determination of 14n:15n ratios. the molar ratios of acetylene to nitrogen varied greatly from the theoretical value.1977614010
effects of low concentrations of bisulfite-sulfite and nitrite on microorganisms.a wide range of microorganisms was tested to determine their sensitivity to low concentrations of bisulfite-sulfite and nitrite, solubility products of so2 and no2, respectively. photosynthesis by blue-green algae (cyanobacteria) was more strongly inhibited by 0.1 mm bisulfite-sulfite and 1 mm nitrite at ph 6.0 than photosynthesis by eucaryotic algae and respiration of bacteria, fungi, and protozoa. at ph 7.7, blue-green algae were still more sensitive to bisulfite-sulfite and nitrite than eucar ...1978646357
[studies on the effects of atrazine and simazine on the n-binding bacteria azotobacter and beijerinckia in the ferralitic soils of cuba].in two cuban locations (santiago de las vegas and jovellanos) the effect was investigated of increasing atrazin and simazin applications (0, 4, 8, 16 kg/ha) to the number of azotobacter and beijerinckia colonies in weakly ferrallitic soils 7 and 15 days after their application during the rainy and dry seasons. the results were as follows: 1. the number of the beijerinckia colonies is higher in all variants than that of azotobacter. 2. beijerinckia and azotobacter are stimulated up to 7 days, par ...1978728049
oxidation of the carcinogens benzo [a] pyrene and benzo [a] anthracene to dihydrodiols by a bacterium.a mutant strain of beijerinckia, after growth with succinate plus biphenyl, contains an enzyme system that oxidizes benzo [a] pyrene and benzo [a] anthracene to mixtures of vicinal dihydrodiols. the major dihydrodiol formed from benzo [a] pyrene was identified as cis-9, 10-dihydroxy-9, 10-dihydrobenzo [a] pyrene by comparison with a synthetic sample. benzo [a] anthracene was metabolized to four dihydrodiols, the major isomer being cis-1, 2-dihydroxy-1, 2-dihydroxy-1, 2-dihydrobenzo [a] anthracen ...19751145203
beijerinckia indica var. penicillanicum penicillin v acylase: enhanced enzyme production by catabolite repression-resistant mutant and effect of solvents on enzyme activity.beijerinckia indica var. penicillanicum mutant urems-5, producing 168% more penicillin v acylase, was obtained by successive treatment with uv, gamma-irradiation and ethylmethane sulfonate. penicillin v acylase production by the mutant strain was resistant to catabolite repression by glucose. incorporation of glucose, sodium glutamate and vegetable oils in the medium enhanced enzyme production. the maximum specific production of penicillin v acylase was 244 iu/g dry weight of cells. effect of so ...19911367509
bacterial oxidation of chemical carcinogens: formation of polycyclic aromatic acids from benz[a]anthracene.a beijerinckia strain designated strain b1 was shown to oxidize benz[a]anthracene after induction with biphenyl, m-xylene, and salicylate. biotransformation experiments showed that after 14 h a maximum of 56% of the benz[a]anthracene was converted to an isomeric mixture of three o-hydroxypolyaromatic acids. nuclear magnetic resonance and mass spectral analyses led to the identification of the major metabolite as 1-hydroxy-2-anthranoic acid. two minor metabolites were also isolated and identified ...19882462407
[subordination of the taxa of gram-negative bacteria determined by numerical analysis methods].various numerical methods were used to estimate the coordination of taxa of gram-negative aerobic and facultative anaerobic organoheterotrophic and chemolithotrophic bacteria. stable phena were found to be formed by cultures belonging to the families rhizobiaceae, halobacteriaceae, enterobacteriaceae, nitrobacteriaceae (except the genus nitrobacter), and methylomonadaceae (except the genus methylococcus). the unstable position was found in the genera thermus, zoogloea, xanthomonas, sulfolobus, m ...19863523170
role of dissolution rate and solubility in biodegradation of aromatic compounds.strains of moraxella sp., pseudomonas sp., and flavobacterium sp. able to grow on biphenyl were isolated from sewage. the bacteria produced 2.3 to 4.5 g of protein per mol of biphenyl carbon, and similar protein yields were obtained when the isolates were grown on succinate. mineralization of biphenyl was exponential during the phase of exponential growth of moraxella sp. and pseudomonas sp. in biphenyl-supplemented media, flavobacterium sp. had one exponential phase of growth apparently at the ...19873566268
oxidation of biphenyl by a beijerinckia species. 19734689045
effect of lyophilization on morphological and physiological properties of some strains of azotobacter and beijerinckia. 19734701338
[study of ca requirement in beijerinckia]. 19695363516
an extracellular polysaccharide from beijerinckia indica, containing l-guluronic acid residues. 19705487828
occurrence of poly-beta-hydroxybutyrate in the azotobacteriaceae.poly-beta-hydroxybutyrate (phb) from various representative strains of the genera azotobacter, beijerinckia, and derxia was isolated and characterized. during growth in shake culture, with glucose as a carbon and energy source, and molecular nitrogen as a nitrogen source, increase in dry weight appeared linear, and phb formed a constant percentage of the dry weight. in a medium containing 1% (w/v) glucose, phb declined with the onset of the stationary phase of growth; with 2% (w/v) glucose, an i ...19685650085
[experimental study on beijerinckia-lipomyces synergism in nitrogen fixation]. 19685758610
identification of beijerinckia from pacific northwest soils. 19665937254
bacterial oxidation of the polycyclic aromatic hydrocarbons acenaphthene and acenaphthylene.a beijerinckia sp. and a mutant strain, beijerinckia sp. strain b8/36, were shown to cooxidize the polycyclic aromatic hydrocarbons acenaphthene and acenaphthylene. both organisms oxidized acenaphthene to the same spectrum of metabolites, which included 1-acenaphthenol, 1-acenaphthenone, 1,2-acenaphthenediol, acenaphthenequinone, and a compound that was tentatively identified as 1,2-dihydroxyacenaphthylene. in contrast, acenaphthylene was oxidized to acenaphthenequinone and the compound tentativ ...19846089663
purification of the rice embryo lectin and its binding to nitrogen-fixing bacteria from the rhizosphere of rice.a lectin was purified from rice embryos by aqueous acid extraction of crude embryo powder, followed by ammonium sulfate precipitation, affinity chromatography on agarose p-aminophenyl-beta-d-n-acetylglucosamine and gel-filtration on aca 54. its homogeneity was checked by polyacrylamide gel electrophoresis, gel-filtration and immunological methods. the hemagglutinating activity of the purified rice lectin was 0.02 micrograms/ml. this lectin labelled with [14c] acetic anhydride was shown to intera ...19846712642
[species of the genus beijerinckia derx isolated from a soil of corrientes (argentina)].four species of bacteria were isolated from one soil of corrientes (argentina). they were first classified as beijerinckia genus based on their cultural, morphological and physiological characteristics. the basic criteria were: growth media without combined nitrogen and fixation of high amounts of gaseous nitrogen (as determined by the acetylene-ethylene assay). further studies permitted the classification of these microorganisms in the following species: beijerinckia indica, beijerinckia flumin ...19806755550
plasmid involvement in the degradation of polycyclic aromatic hydrocarbons by a beijerinckia species.a beijerinckia species, capable of oxidizing phenanthrene, biphenyl and other polycyclic aromatic hydrocarbons, was shown to contain two plasmids that were designated pkgl and pkg2. the molecular masses of plasmids pkg1 and pkg2, as determined by electron microscopy, were approximately 147 x 10(6) and 20.8 x 10(6) daltons, respectively. growth of the organism on benzoate led to the isolation of strains that had lost the ability to grow with phenanthrene and biphenyl. all of the phn-, bph- strain ...19836838595
molecular and biochemical characterization of two meta-cleavage dioxygenases involved in biphenyl and m-xylene degradation by beijerinckia sp. strain b1.beijerinckia sp. strain b1 is able to grow on either biphenyl or m-xylene as the sole source of carbon and is capable of cooxidizing many polycyclic aromatic hydrocarbons. the catabolic pathways for biphenyl and m-xylene degradation are coinduced and share common downstream enzymatic reactions. the catabolic pathway for biphenyl degradation involves two meta-cleavage steps, one for 2,3-dihydroxybiphenyl and a second for catechol. the catabolic pathway for m-xylene involves one m-cleavage step fo ...19957768806
oxidation of 2-methoxynaphthalene by toluene, naphthalene and biphenyl dioxygenases:structure and absolute stereochemistry of metabolites.2-methoxynaphthalene was subjected to biooxidation by whole cells of six organisms: pseudomonas putida f39/d containing toluene dioxygenase, escherichia coli jm109(pdtg601), containing recombinant toluene dioxygenase from pp f39/d, pseudomonas sp. ncib 9816/11, containing naphthalene dioxygenase. e. coli jm109(pdtg141), containing recombinant naphthalene dioxygenase from ncib 98161/11, e. coli c534(pror/sac) containing recombinant naphthalene dioxygenase from pp g7, and beijerinckia sp. b8/36, c ...19947858982
oxidation of carbazole to 3-hydroxycarbazole by naphthalene 1,2-dioxygenase and biphenyl 2,3-dioxygenase.naphthalene 1,2-dioxygenase from pseudomonas sp. ncib 9816-4 and biphenyl dioxygenase from beijerinckia sp. b8/36 oxidized the aromatic n-heterocycle carbazole to 3-hydroxycarbazole. toluene dioxygenase from pseudomonas putida f39/d did not oxidize carbazole. transformations were carried out by mutant strains which oxidize naphthalene and biphenyl to cis-dihydrodiols, and with a recombinant e. coli strain expressing the structural genes of naphthalene 1,2-dioxgenase from pseudomonas sp. ncib 981 ...19938270195
sequence and expression of an isocitrate dehydrogenase-encoding gene from a polycyclic aromatic hydrocarbon oxidizer, sphingomonas yanoikuyae b1.an 18.5-kb dna fragment was cloned from sphingomonas yanoikuyae (sy) b1 (previously beijerinckia b1). analysis of a 4.3-kb sequence revealed an isocitrate dehydrogenase (idh)-encoding gene (idha), an unidentified open reading frame (orf) and a partial glucosamine synthetase-encoding orf (glms). as in a number of bacteria, tn7 insertion was found specifically at a site past the stop codon of glms. the predicted 406-amino-acid sequence of idha shows, for the first time, an extensive sequence ident ...19968626059
oxidation of 6,7-dihydro-5h-benzocycloheptene by bacterial strains expressing naphthalene dioxygenase, biphenyl dioxygenase, and toluene dioxygenase yields homochiral monol or cis-diol enantiomers as major products.bacterial strains expressing naphthalene, biphenyl, and toluene dioxygenase were examined for their abilities to oxidize 6,7-dihydro-5h-benzocycloheptene (benzocyclohept-1-ene). the major oxidation products were isolated, and their absolute configurations were determined by chiral 1h nuclear magnetic resonance analysis of diastereomeric boronate esters, chiral stationary-phase high-pressure liquid chromatography, and stereo-chemical correlation. pseudomonas sp. strain 9816/11 and sphingomonas ya ...19968919798
reclassification of a polycyclic aromatic hydrocarbon-metabolizing bacterium, beijerinckia sp. strain b1, as sphingomonas yanoikuyae by fatty acid analysis, protein pattern analysis, dna-dna hybridization, and 16s ribosomal dna sequencing.a bacterium isolated from a polluted stream, capable of metabolizing biphenyl, naphthalene, phenanthrene, and higher-molecular-weight polycyclic aromatic hydrocarbons (d. gibson, v. mahadevan, d. jerina, h. yagi, and h. yeh, science 189:295-297, 1975), was previously identified as beijerinckia sp. strain b1. in this investigation, 16s rrna gene sequencing, biochemical tests, fatty acid methyl ester analysis, polyacrylamide gel electrophoresis of protein, and dna-dna hybridization were used to de ...19968934904
structural studies of cv-70 polysaccharide.the goal of this paper is the characterization of the chemical structure of the water-soluble polysaccharide, cv-70, produced by bacteria beijerinckia sp. beijerinckia sp. is a genus of gram-negative, aerobic bacteria, usually found in sugar cane root. the cv-70 polysaccharide was produced in a fermentation medium containing 5% sucrose as the carbon source, tryptose and salts, at 25 degrees c [1]. the polysaccharide was hydrolyzed with 2 n trifluoroacetic acid at 100 degrees c for 16 h, purified ...19979283025
isolation of acidophilic methane-oxidizing bacteria from northern peat wetlands.acidic northern wetlands are an important source of methane, one of the gases that contributes to global warming. methane oxidation in the surface of these acidic wetlands can reduce the methane flux to the atmosphere up to 90 percent. here the isolation of three methanotrophic microorganisms from three boreal forest sites is reported. they are moderately acidophilic organisms and have a soluble methane monooxygenase. in contrast to the known groups of methanotrophs, 16s ribosomal dna sequence a ...19989765151
[physiologo-biochemical properties of a strain of beijerinckia mobilis 1phi phn+--a degrader of polycyclic aromatic hydrocarbons].beijerinckia mobilis 1f capable of degrading polycyclic aromatic hydrocarbons (pahs) was isolated from a soil contaminated with creosote. strain 1f could utilize phenanthrene and naphthalene as the sole sources of carbon. the mean rate of phenanthrene degradation during culture growth was 7-8 micrograms/(ml h). after cultivation under nonselective conditions, strain 1f retained its ability to degrade phenanthrene. cometabolism considerably widened the range of pahs that could be transformed by s ...199910734634
methylocella palustris gen. nov., sp. nov., a new methane-oxidizing acidophilic bacterium from peat bogs, representing a novel subtype of serine-pathway methanotrophs.a new genus, methylocella, and a new species, methylocella palustris, are proposed for three strains of methane-oxidizing bacteria isolated from acidic sphagnum peat bogs. these bacteria are aerobic, gram-negative, colourless, non-motile, straight and curved rods that utilize the serine pathway for carbon assimilation, multiply by normal cell division and contain intracellular poly-beta-hydroxybutyrate granules (one at each pole). these strains use methane and methanol as sole sources of carbon ...200010843033
[a system of oligonucleotide primers for amplifying nifh genes from various taxonomic groups of prokaryotes].based on the analysis of the nifh gene nucleotide sequences from genbank, a system of primers was developed that makes it possible to obtain 370- and 470-bp pcr fragments of the nifh gene of nitrogen-fixing bacteria and archaea. the effectiveness of the proposed system for revealing the presence of nifh genes was demonstrated by pcr on the dna isolated from nitrogen-fixing prokaryotes for which the primary structure of these genes is known and which belong to different taxonomic groups. nifh seq ...200111338842
beijerinckia sp strain b1: a strain by any other name.beijerinckia sp strain b1 grows with biphenyl as its sole source of carbon and energy. a mutant, strain b8/36, oxidized biphenyl to cis-(2s,3r)-dihydroxy-l-phenylcyclohexa-4,6-diene (cis-biphenyl dihydrodiol). strain b8/36 oxidized anthracene, phenanthrene, benz[a]anthracene and benzo[a]pyrene to cis-dihydrodiols. other substrates oxidized to cis-dihydrodiols were dibenzofuran, dibenzothiophene and dibenzo-p-dioxin. biphenyl dioxygenase activity was observed in cells of beijerinckia b1 and b8/36 ...199911423945
[the growth-promoting effect of beijerinckia and clostridium sp. cultures on some agricultural crops].new strains of beijerinckia mobilis and clostridium sp. isolated from the pea rhizosphere were studied with respect to their promoting effect on the growth and development of some agricultural crops. seed soaking in bacterial suspensions followed by the soil application of the suspensions or their application by means of foliar spraying was found to be the most efficient method of bacterization. the application of b. mobilis and clostridium sp. cultures in combination with mineral fertilizers in ...200211910801
beijerinckia derxii releases plant growth regulators and amino acids in synthetic media independent of nitrogenase activity.this study aims at evaluating the ability of beijerinckia derxii, a free-living nitrogen (n)-fixing bacterium frequently isolated from tropical soils, to release certain plant growth regulators [indoleacetic acid (iaa), ethylene, polyamines] and amino acids into the growth medium.200312969294
methylocella silvestris sp. nov., a novel methanotroph isolated from an acidic forest cambisol.two strains of gram-negative, aerobic, non-pigmented, non-motile, rod-shaped, methane-oxidizing bacteria were isolated from an acidic forest cambisol near marburg, germany, and were designated as strains bl2(t) and a1. these bacteria were morphologically and phenotypically similar to methylocella palustris k(t). the cells possess a highly specific bipolar appearance. they lack the intracytoplasmic membranes common to all methane-oxidizing bacteria except methylocella, but contain a vesicular mem ...200313130000
[distribution of azotobacter and beijerinckia in the principal types of soil from west africa]. 196314048710
[behavior of nitrogen-fixing bacteria of the beijerinckia genus as a function of ph and calcium]. 196414137366
nitrogen fixation by beijerinckia in relation to slime formation. 196414245148
azotobacter and beijerinckia in the soils and rhizosphere of plants in egypt. 196514339686
activity and survival of spray-dried beijerinckia sp. microencapsulated in different carbohydrates.this study examined the possibility of preserving beijerinckia cultures by encapsulation using a spray drier, for use in biotechnological processes in the production of biopolymers. an adequate choice of the wall (coating) material is one of the factors that will determine the degree of cell survival and the maintenance of fermentative activity in the encapsulated inoculum. malt dextrin, dehydrated glucose syrups, modified starch, and acacia (gum arabic) were used as wall materials. the results ...200314646002
during stationary phase, beijerinckia derxii shows nitrogenase activity concomitant with the release and accumulation of nitrogenated substances.beijerinckia derxii, a free-living nitrogen-fixing bacterium, maintained an increasing nitrogenase specific activity during the stationary growth phase. to verify the destination of the nitrogen fixed during this phase, intra and extracellular nitrogenated contents were analyzed. organic nitrogen and amino acids were detected in the supernatant of the cultures. an increase in intracellular content of both nitrogen and protein occurred. cytoplasmic granules indicated the presence of arginine. the ...200314717451
survival of beijerinckia sp. microencapsulated in carbohydrates by spray-drying.the encapsulation of beijerinckia sp. cell suspension in different wall materials using the spray drying technique was performed. mat dextrin, dehydrated glucose syrups, gum acacia and modified starch materials were tested. cell viability assays were carried out before and after drying and during storage of the products. the surface area and characteristics of the encapsulated powders were examined using bet adsorption of n(2) and scanning electron microscopy, respectively. the residual moisture ...200414718182
nifh and nifd phylogenies: an evolutionary basis for understanding nitrogen fixation capabilities of methanotrophic bacteria.the ability to utilize dinitrogen as a nitrogen source is an important phenotypic trait in most currently known methanotrophic bacteria (mb). this trait is especially important for acidophilic mb, which inhabit acidic oligotrophic environments, highly depleted in available nitrogen compounds. phylogenetically, acidophilic mb are most closely related to heterotrophic dinitrogen-fixing bacteria of the genus beijerinckia: to further explore the phylogenetic linkage between these metabolically diffe ...200415133093
methylotrophic autotrophy in beijerinckia mobilis.representatives of the genus beijerinckia are known as heterotrophic, dinitrogen-fixing bacteria which utilize a wide range of multicarbon compounds. here we show that at least one of the currently known species of this genus, i.e., beijerinckia mobilis, is also capable of methylotrophic metabolism coupled with the ribulose bisphosphate (rubp) pathway of c1 assimilation. a complete suite of dehydrogenases commonly involved in the sequential oxidation of methanol via formaldehyde and formate to c ...200515901717
history on the biological nitrogen fixation research in graminaceous plants: special emphasis on the brazilian experience.this review covers the history on biological nitrogen fixation (bnf) in graminaceous plants grown in brazil, and describes research progress made over the last 40 years, most of which was coordinated by johanna döbereiner. one notable accomplishment during this period was the discovery of several nitrogen-fixing bacteria such as the rhizospheric (beijerinckia fluminensis and azotobacter paspali), associative (azospirillum lipoferum, a. brasilense, a. amazonense) and the endophytic (herbaspirillu ...200516127558
[methanol and glucose metabolism in beijerinckia mobilis]. 200516315991
identification of beijerinckia in the high arctic (devon island, northwest territories).although generally considered, with few exceptions, to be restricted to the acidic, tropical soils of the southern hemisphere, beijerinckia species, resembling b. indica, were found at three sites on devon island (75 degrees 33'n, 84 degrees 40'w) in the canadian arctic.197816345263
metabolism of dibenzo-p-dioxin and chlorinated dibenzo-p- dioxins by a beijerinckia species.whole cells of the parent strain of beijerinckia, grown with succinate and biphenyl, oxidized dibenzo-p-dioxin and several chlorinated dioxins. the rate of oxidation of the chlorinated dibenzo-p-dioxins decreased with an increasing degree of chlorine substitution. a mutant strain (b8/36) of beijerinckia oxidized dibenzo-p-dioxin to cis-1,2-dihydroxy-1,2-dihydrodibenzo-p-dioxin. the mutant organism also oxidized two monochlorinated dibenzo-p-dioxins to cis-dihydrodiols. no metabolites were detect ...198016345500
rapid screen for bacteria degrading water-insoluble, solid hydrocarbons on agar plates.a rapid procedure was devised for detecting on solid media bacteria able to degrade water-insoluble, solid hydrocarbons such as the polycyclic aromatic hydrocarbons phenanthrene, anthracene, and biphenyl. after alcaligenes faecalis afk2 was inoculated on a plate containing mineral salts agar, an ethereal solution of phenanthrene (about 10%, wt/vol) was sprayed on the surface of the plate, and the plate was incubated at 30 degrees c for 2 to 3 days. colonies showing degradation were surrounded wi ...198216345951
comparative cytochrome oxidase and superoxide dismutase analyses on strains of azotobacter vinelandii and other related free-living nitrogen-fixing bacteria.quantitative n,n,n',n'-tetramethyl-p-phenylenediamine (tmpd) oxidase and superoxide dismutase (sod) analyses were performed on representative organisms of the family azotobacteraceae. azotobacter vinelandii, azotobacter chroococcum, azotobacter paspali, and derxia gummosa exhibited high quantitative tmpd oxidase activities, and their extracts possessed very active and electrophoretically homogeneous (single gel band) fe-type sods. azomonas macrocytogenes extracts had similar single fe-type sods, ...198416346548
phyllosphere of cotton as a habitat for diazotrophic microorganisms.positive nitrogenase activities ranging from 0.18 to 0.78 nmol of c(2)h(4) cm h were detected on the leaf surfaces of different varieties of cotton (gossypium hirsutum l. and g. herbaceum l.) plants. beijerinckia sp. was observed to be the predominant nitrogen-fixing microorganism in the phyllosphere of these varieties. a higher level of phyllosphere nitrogen-fixing activity was recorded in the variety varalaxmi despite a low c/n ratio in the leaf leachates. leaf surfaces of the above variety po ...198416346639
pseudolabrys taiwanensis gen. nov., sp. nov., an alphaproteobacterium isolated from soil.a gram-negative, short rod-shaped micro-organism (cc-bb4(t)) was isolated on nutrient agar from soil from sinshe in taichung county, taiwan. analysis of the 16s rrna gene sequence demonstrated that this isolate was novel, as it showed <92 % similarity to the sequences of species of the genera labrys, beijerinckia and methylocystis. the micro-organism did not utilize methylamine or methanol as a substrate, but was able to use several organic acids. the fatty acid profile was different from those ...200617012581
poly(3-hydroxybutyrate) granules at the early stages of formation are localized close to the cytoplasmic membrane in caryophanon latum.localization of newly synthesized poly(3hydroxybutyrate) (phb) granules was determined by confocal laser scanning fluorescence microscopy of nile red-stained cells and by transmission electron microscopy (tem). phb granules of nile red-stained living cells of caryophanon latum at the early stages of phb accumulation were frequently found at or close to the cytoplasmic membrane. tem analysis of the same culture revealed electron-translucent globular structures resembling phb granules that were no ...200717085698
physicochemical and biological characteristics of deae-derivatized ps7 biopolymer of beijerinckia indica.physicochemical and biological characteristics of the exopolysaccharide, ps7, produced from beijerinckia indica were investigated. the ps7 weight fractions of glc and glcua were 0.45 and 0.25, respectively, and the molar ratio of glc:rha:galua was approximately 5:1:1.3. the ps7 was chemically derivatized with diethylaminoethyl chloride-hcl (deae-hcl), and the resulting modified ps7 contained both positive and negative charges. the elemental and ir analyses were conducted to confirm the successfu ...200717316786
structural investigations of the ferredoxin and terminal oxygenase components of the biphenyl 2,3-dioxygenase from sphingobium yanoikuyae b1.the initial step involved in oxidative hydroxylation of monoaromatic and polyaromatic compounds by the microorganism sphingobium yanoikuyae strain b1 (b1), previously known as sphingomonas yanoikuyae strain b1 and beijerinckia sp. strain b1, is performed by a set of multiple terminal rieske non-heme iron oxygenases. these enzymes share a single electron donor system consisting of a reductase and a ferredoxin (bpdo-fb1). one of the terminal rieske oxygenases, biphenyl 2,3-dioxygenase (bpdo-ob1), ...200717349044
phenanthrene degradation by beijerinckia sp. b8/36. 198618553858
identification of beijerinckia fluminensis strains cip 106281t and uqm 1685t as rhizobium radiobacter strains, and proposal of beijerinckia doebereinerae sp. nov. to accommodate beijerinckia fluminensis lmg 2819.during the course of a research project with free-living, nitrogen-fixing bacteria, we determined the 16s rrna gene sequence of beijerinckia fluminensis strains uqm 1685t and cip 106281t and discovered that they were only 90.6-91.2% similar to the sequences of strains of other beijerinckia species and subspecies. moreover, the highest similarity to these sequences (99.7%) corresponded to strains of rhizobium radiobacter (including agrobacterium tumefaciens). other diagnostic features confirmed t ...200919620377
complete genome sequence of beijerinckia indica subsp. indica.beijerinckia indica subsp. indica is an aerobic, acidophilic, exopolysaccharide-producing, n(2)-fixing soil bacterium. it is a generalist chemoorganotroph that is phylogenetically closely related to facultative and obligate methanotrophs of the genera methylocella and methylocapsa. here we report the full genome sequence of this bacterium.201020601475
methyloferula stellata gen. nov., sp. nov., an acidophilic, obligately methanotrophic bacterium possessing only a soluble methane monooxygenase.two strains of aerobic methanotrophic bacteria, ar4(t) and sop9, were isolated from acidic (ph 3.8-4.0) sphagnum peat bogs in russia. another phenotypically similar isolate, strain lay, was obtained from an acidic (ph 4.0) forest soil in germany. cells of these strains are gram-negative, non-pigmented, non-motile, thin rods that multiply by irregular cell division and form rosettes or amorphous cell conglomerates. similar to methylocella spp., strains ar4(t), sop9 and lay possess only a soluble ...201021097638
ability of beijerinckia indica to degrade phenanthrene and reduce hydraulic conductivity.this study evaluates the ability of beijerinckia indica (b. indica) as a biomaterial for aerobic biofilm barriers. b. indica's ability to remove phenanthrene was measured using a two-phase partitioning bioreactor. approximately 500 mg/l of phenanthrene was gradually removed over a two week period under aerobic conditions of the bioreactor. b. indica's ability to reduce hydraulic conductivity was evaluated using rigid wall soil columns inoculated with the bacteria. b. indica formed a large quanti ...201021123927
biofilm coupled with uv irradiation for phenol degradation and change of its community structure.the extensive use of phenol compounds and the inability to remove these compounds during wastewater treatment have resulted in the widespread occurrence of phenols in the natural environment. phenols have been linked to serious risks to human and environmental health. hence, the need to develop technologies that can effectively remove phenols from wastewater and source waters is a pressing challenge. in this study, light ceramic particles were immersed in activated sludge acclimated to degrade p ...201121234766
the status of the species beijerinckia fluminensis dobereiner and ruschel 1958. request for an opinion.in a previous article (oggerin et al., 2009) it has been shown that strain [beijerinckia fluminensis] uqm 1685t, and its derived equivalent [b. fluminensis] cip 106281t, do not conform to the description of the type strain of beijerinckia fluminensis döbereiner and ruschel 1958. indeed, both were identified as species rhizobium radiobacter strains and exhibited marked phenotypic and genotypic differences with beijerinckia spp. for which it can be concluded that both strains, and any other equiva ...201121515702
Nitrogen sources of Oligoporus placenta and Trametes versicolor evaluated in a 2(3) experimental plan.Four full-factorial 2(3) experimental plans were applied to evaluate the nitrogen (N) sources of Oligoporus placenta and Trametes versicolor and their interaction with the atmospheric N(2)-assimilating bacterium Beijerinckia acida. The effects of N from peptone, of sapwood and of N from gaseous N(2) on fungal, bacterial and fungal-bacterial activity were investigated. The activities were determined by quantification of biomass, formation of CO(2), consumption of O(2) and laccase activity. The si ...201222208603
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