seven cases of bacteremia due to ochrobactrum anthropi. 19921457654
structural similarity of d-aminopeptidase to carboxypeptidase dd and beta-lactamases.the gene for d-aminopeptidase (dap) has been isolated from the bacterium ochrobactrum anthropi scrc c1-38 [asano, y., nakazawa, a., kato, y., & kondo, k. (1989) j. biol. chem. 264, 14233-14239] and its nucleotide sequence determined. an expression plasmid pc138dp (4.5 kb) was constructed by placing the gene downstream of the lac promoter of puc19. the amount of the enzyme in the cell-free extract of escherichia coli jm109/pc138dp was elevated to 288,000 units/l of culture, which is about 3600-fo ...19921540587
catheter-associated sepsis caused by ochrobactrum anthropi: report of a case and review of related nonfermentative bacteria.ochrobactrum anthropi, formerly known as cdc group vd, is an oxidase-producing, gram-negative, non-lactose-fermenting bacillus that oxidizes glucose and grows readily on macconkey agar. only occasionally isolated from human clinical specimens, this organism has rarely been found to be pathogenic. we describe the first reported case of infection due to o. anthropi in a child, that of bacteremia in a 3-year-old girl undergoing chemotherapy for retinoblastoma. in addition, we review the literature ...19921576286
a new d-stereospecific amino acid amidase from ochrobactrum anthropi.a new d-stereospecific amino acid amidase has been partially purified from ochrobactrum anthropi scrc sv3, which had been isolated and selected from soil. the mr of the enzyme was estimated to be about 38,000, and its isoelectric point was 5.3. the enzyme catalyzes the stereospecific hydrolysis of d-amino acid amide to yield d-amino acid and ammonia. the major substrates included d-phenylalanine amide, d-tyrosine amide, d-tryptophan amide, d-leucine amide, and d-alanine amide.19892751665
properties of a novel d-stereospecific aminopeptidase from ochrobactrum anthropi.a novel aminopeptidase active toward d-amino acid-containing peptides, d-amino acid amides, and d-amino acid esters has been purified 2,800-fold to homogeneity from a bacterium ochrobactrum anthropi scrc c1-38, which had been isolated from soil. the enzyme has a molecular weight of about 122,000 and is composed of two identical subunits (mr = 59,000). the optimal ph for activity was 8.0. it showed strict d-stereospecificity toward substrates including low molecular weight d-amino acid amides suc ...19892760064
specific detection of brucella dna by pcr.a pcr assay with primers derived from the 16s rrna sequence of brucella abortus was developed. nine different combinations between six primers were tested. one pair of primers, which amplified a 905-bp fragment, was selected. as little as 80 fg of brucella dna was detected by this method. dnas from all of the representative strains of the species and biovars of brucella and from 23 different brucella isolates were analyzed and yielded exclusively the 905-bp fragment. no amplification was detecte ...19957538508
[comparative susceptibility of ochrobactrum anthropi, agrobacterium tumefaciens, alcaligenes faecalis, alcaligenes denitrificans subsp. denitrificans, alcaligenes denitrificans subsp. xylosidans and bordetella bronchiseptica against 35 antibiotics including 17 beta-lactams].ochrobactrum anthropi, formerly known as "achromobacter sp." or cdc group vd has been isolated from water, hospital environment (antiseptic solutions, dialysis fluids ... ). o. anthropi is a gram negative, motile, strictly aerobic, oxydase positive and non-fermentative bacteria with a strong urease activity. the susceptibility of 13 strains of o. anthropi was determined by agar diffusion method and compared to those of type strains of agrobacterium tumefaciens, alcaligenes faecalis, alcaligenes ...19957567111
degradation of 4-chloro-2-methylphenol by an activated sludge isolate and its taxonomic description.the gram-negative strain s1, isolated from activated sludge, metabolized 4-chloro-2-methylphenol by an inducible pathway via a modified ortho-cleavage route as indicated by a transiently secreted intermediate, identified as 2-methyl-4-carboxymethylenebut-2-en-4-olide by gas chromatography/mass spectrometry. beside 4-chloro-2-methylphenol only 2,4-dichlorophenol and 4-chlorophenol were totally degraded, without an accumulation of intermediates. the chlorinated phenols tested induced activities of ...19957772944
differentiation of brucella abortus bv. 1, 2, and 4, brucella melitensis, brucella ovis, and brucella suis bv. 1 by pcr.several pcr assays which identify the genus brucella but do not discriminate among species have been reported. we describe a pcr assay that comprises five oligonucleotide primers which can identify selected biovars of four species of brucella. individual biovars within a species are not differentiated. the assay can identify three biovars (1, 2, and 4) of b. abortus, all three biovars of b. melitensis, biovar 1 of b. suis, and all b. ovis biovars. these biovars include all of the brucella specie ...19947852552
activation and cytotoxicity of 2-alpha-aminoacyl prodrugs of an effort to improve the selectivity of the anticancer drug methotrexate (mtx), a series of potential prodrugs in which the 2-amino group was acylated with various alpha-amino acids (as well as l-pyroglutamic acid) was synthesized. such derivatives are anticipated to be hydrolysed to mtx by appropriate aminopeptidases localized (over-expressed naturally or targeted as anti-tumor antibody conjugates) in the vicinity of the tumor. the l-leucyl, l-valyl, l-isoleucyl, d-alanyl and l-pyroglutamyl ...19957872963
an outbreak of ochrobactrum anthropi bacteraemia in five organ transplant patients.nosocomial bacteraemia caused by ochrobactrum anthropi occurred over a 1-month period in five organ transplant recipients, four of whom were in the same renal and pancreatic transplant unit. bacteraemia occurred with cyclosporin a, azathioprine and steroids, and with a rabbit anti-thymocyte globulin (ratg) during the induction phase. ratg appeared to be the only common factor among the five cases. over the period described, 71.4% of all patients receiving ratg developed o. anthropi bacteraemia. ...19947916361
bacteremia caused by ochrobactrum anthropi in an immunocompromised child. 19948002092
infections with the unusual human pathogens agrobacterium species and ochrobactrum anthropi.agrobacterium species and ochrobactrum anthropi are generally considered innocuous in clinical settings, yet during the last decade a number of sporadic cases of human infection due to these organisms have been reported. we studied nine cases of infection (septicemia and peritonitis) caused by agrobacterium-like microorganisms in eight patients. all patients were immunocompromised and had permanent central venous or peritoneal dialysis catheters in place. seven patients were women, and eight inf ...19948086552
necrotizing fasciitis, bacteremia, and multiorgan failure caused by ochrobactrum anthropi. 19938218705
ochrobactrum anthropi bacteremia: report of four cases and short review.ochrobactrum anthropi, formerly "achromobacter" cdc group vd, is a nonfermentative, nonfastidious gram-negative bacillus, that only recently has been given attention as a potential human pathogen. over a 2-year period, we observed four patients with multiple blood cultures that were positive for the organism. the patients had acute leukemia as underlying disease, and presented with clinical and microbiologic features consistent with catheter-related bacteremia. in three of the patients the infec ...19938300247
ochrobactrum anthropi bacteremia in a child. 19938405587
ochrobactrum anthropi meningitis in pediatric pericardial allograft transplant epidemiologic investigation was done after 3 patients contracted ochrobactrum anthropi meningitis at one hospital in october 1994. neurosurgical patients with pericardial tissue implants were at greater risk of infection than other neurosurgical patients (3/14 vs. 0/566; p<.001). cultures of implants removed from 2 case-patients, an implant at implantation, a nonimplanted pericardial tissue, and an unwrapped but unopened bottle of hank's balanced salt solution (hbss) grew o. anthropi. patient ...19968627029
evaluation of pulsed-field gel electrophoresis and rep-pcr for the epidemiological analysis of ochrobactrum anthropi strains.pulsed-field gel electrophoresis and polymerase chain reaction genome fingerprinting based on repetitive chromosomal sequences (rep-pcr) were used for typing 14 strains of ochrobactrum anthropi. six strains isolated during an outbreak of bacteraemia in patients who had received a contaminated rabbit anti-thymocyte globulin gave identical patterns by both techniques. different patterns were found in sporadic and reference strains, except for one clinical isolate received from another hospital tha ...19958681989
ochrobactrum anthropi endophthalmitis after uncomplicated cataract treat a case of ochrobactrum anthropi endophthalmitis after uneventful cataract surgery.19968694102
pyogenic infections due to ochrobactrum anthropi.ochrobactrum anthropi is a nonfermentative gram-negative bacillus that has been isolated with increasing frequency from human clinical specimens. previously, its pathogenic niche was believed to involve the causation of catheter-associated bacteremic illnesses. we describe three cases of pyogenic infection due to o. anthropi, thereby expanding the known pathogenic potential of this organism.19968722944
determination of the o-specific polysaccharide structure in the lipopolysaccharide of ochrobactrum anthropi lmg 3331. 19968765064
ochrobactrum anthropi meningitis associated with cadaveric pericardial tissue processed with a contaminated solution--utah, 1994.from october 22 through november 3, 1994, three cases of ochrobactrum anthropi meningitis were diagnosed among pediatric patients at a hospital in utah (hospital a). the three patients had undergone neurosurgical procedures in which pericardial grafts processed at hospital b were used to close defects of the dura mater. this report summarizes the case investigations, which document that the cases resulted from human pericardial tissue grafts contaminated with o. anthropi.19968769657
from the centers for disease control and prevention. ochrobactrum anthropi meningitis associated with cadaveric pericardial tissue processed with a contaminated solution--utah, 1994. 19968773623
effects of atrazine on ochrobactrum anthropi membrane fatty acids.ochrobactrum anthropi is a gram-negative bacillus recognized as a human opportunist pathogen isolated in clinical specimens and not of clinical significance. we report a new aspect of this bacterium, that it has been isolated from activated sludge. in fact, it is able to grow on atrazine (2-chloro-4-ethylamino-6-isopropyl-amine-s-triazine) by utilizing it as the only source of carbon. our results show that atrazine (0.03 g/liter) causes a dramatical increase in the degree of saturation of membra ...19968779602
specificity of six gene sequences for the detection of the genus brucella by dna amplification.dna extracted from all brucella species, reference and vaccine strains were amplified by pcr using primers specific for the genes encoding a 31-kda brucella protein, the heat shock proteins (dnaj, dnak, htra and groel) and 16s rna. no difference was found between brucella species and biovars with all primer pairs used, even after restriction enzyme analysis of the amplified fragments. the specificity of the amplified products was confirmed by hybridization with a digoxigenin 3'-labelled specific ...19968810054
ochrobactrum anthropi infection of retained pacemaker leads. 19979114171
endogenous ochrobactrum anthropi describe bilateral endogenous endophthalmitis caused by ochrobactrum anthropi in a partially immunosuppressed patient who had undergone central venous access for hyperalimentation and home intravenous therapy.19979124261
antibody and delayed-type hypersensitivity responses to ochrobactrum anthropi cytosolic and outer membrane antigens in infections by smooth and rough brucella spp.immunological cross-reactions between brucella spp. and ochrobactrum anthropi were investigated in animals and humans naturally infected by brucella spp. and in experimentally infected rams (brucella ovis infected), rabbits (brucella melitensis infected), and mice (b. melitensis and brucella abortus infected). in the animals tested, o. anthropi cytosolic proteins evoked a delayed-type hypersensitivity reaction of a frequency and intensity similar to that observed with b. melitensis brucellin. o. ...19979144364
nuclear magnetic resonance timecourse studies of glyphosate metabolism by microbial soil isolates.1. triple resonance isotope edited nmr spectroscopy (tried) has been developed to detect and examine minute levels of glyphosate metabolites in microbial soil isolates. using stable isotopic labelling (13c and 15n), tried allows the simultaneous detection of multiple metabolites in crude matrices at submicrogram levels. an improvement over earlier techniques where milligrams are needed, tried can detect 500 ng of triply labelled compound in a crude sample (1:14,000 mass ratio) in just hours. 2. ...19979253141
intravenous line infection due to ochrobactrum anthropi (cdc group vd) in a normal host.ochrobactrum anthropi, formerly known as achromobacter species (cdc group vd), is an aerobic, gram-negative bacillus widely distributed in aquatic environments. most important, it has been implicated as a cause of intravenous line infection in immunocompromised hosts with solid tumors or hematologic malignancies. trimethoprim-sulfamethoxazole and aminoglycosides are usually active against o. anthropi, but this organism is usually resistant to beta-lactam antibiotics. because o. anthropi is a low ...19979257145
localization and characterization of a specific linear epitope of the brucella dnak protein.we have previously produced and characterized four monoclonal antibodies to the brucella dnak protein which were derived from mice infected with b. melitensis or immunized with the b. melitensis cell wall fraction. by use of a recombinant dna technique, we have localized a linear epitope, recognized by two of these monoclonal antibodies (v78/07b01/g11 and v78/09d04/d08), in the last 21 amino acids of the c-terminal region of the brucella dnak protein. the c-terminal region has been reported to b ...19979297829
proteomic 'contigs' of ochrobactrum anthropi, application of extensive ph gradients.the most extensive linear ph gradients yet employed in combination with two-dimensional gel electrophoresis are described, along with their application in proteome analysis. a significant proportion of the protein compliment of bacterial species is believed to be accessible using an extended linear ph gradient of 2.3 to 11.0. protein standards with predicted isoelectric points (pi) ranging from 3.24 to 9.56 were used to confirm focusing positions with respect to the immobilised ph gradients (ipg ...19979298651
purification and characterization of a novel glutathione transferase from ochrobactrum anthropi.glutathione transferase was purified from ochrobactrum anthropi and its n-terminal sequence was determined to be mklyykvgacslaphiilseaglpy. the apparent molecular mass of the protein (24 kda) was determined by sds-polyacrylamide gel electrophoresis analysis. the amino acid sequence obtained showed similarities with known bacterial glutathione transferases in the range of 72-64%. immunoblotting experiments performed with antisera raised against glutathione transferase from o. anthropi did not sho ...19989495016
clinical and microbiologic characteristics of ochrobactrum anthropi bacteremia.ochrobactrum anthropi is an oxidase-positive, nonfermenting, gram-negative bacillus that is an emerging pathogen in immunocompromised patients, particularly in those with indwelling central venous catheters. reports of clinical manifestation of o. anthropi bacteremia are rare. herein, we report the clinical and microbiologic characteristics of o. anthropi bacteremia in 15 patients. there were eight males and seven females, whose ages ranged from 1 month to 84 years (mean, 34 years). ten infectio ...19989509845
unconventional genomic organization in the alpha subgroup of the proteobacteria.pulsed-field gel electrophoresis was used to analyze the genomic organization of 16 bacteria belonging or related to the family rhizobiaceae of the alpha subgroup of the class proteobacteria. the number and sizes of replicons were determined by separating nondigested dna. hybridization of an rrn gene probe was used to distinguish between chromosomes and plasmids. members of the genus agrobacterium all possess two chromosomes, and each biovar has a specific genome size. as previously demonstrated ...19989573163
meningitis due to ochrobactrum anthropi: an emerging nosocomial pathogen. a report of 3 cases.we describe 3 cases of ochrobactrum anthropi meningitis following the implantation of pericardial allograft tissue to cover dural defects following craniotomy. following an extensive epidemiologic investigation, the tissue allograft was found to have been contaminated with this unusual organism during the harvesting and processing of the tissue in the tissue bank. this organism was only susceptible to imipenem, tetracycline, gentamicin, and ciprofloxacin. the clinical presentation of these patie ...19979577978
intergeneric transfer of conjugative and mobilizable plasmids harbored by escherichia coli in the gut of the soil microarthropod folsomia candida (collembola).the gut of the soil microarthropod folsomia candida provides a habitat for a high density of bacterial cells (t. thimm, a. hoffmann, h. borkott, j. c. munch, and c. c. tebbe, appl. environ. microbiol. 64:2660-2669, 1998). we investigated whether these gut bacteria act as recipients for plasmids from escherichia coli. filter mating with e. coli donor cells and collected feces of f. candida revealed that the broad-host-range conjugative plasmid prp4-luc (prp4 with a luciferase marker gene) transfe ...19989647844
evaluation of the vitek 2 system for rapid identification of medically relevant gram-negative rods.the new vitek 2 system (biomérieux) was evaluated at two independent sites with the identification card for gram-negative bacilli (id-gnb card). of the 845 strains tested, which represented 70 different taxa belonging to either the family enterobacteriaceae or the nonenteric bacilli, 716 (84.7%) were correctly identified at the species level. thirty-two (3.8%) additional strains were identified to the species level after the performance of simple, rapid manual tests (oxidase, hemolysis, indole r ...19989650942
structural studies on the lipopolysaccharide from a rough strain of ochrobactrum anthropi containing a 2,3-diamino-2,3-dideoxy-d-glucose disaccharide lipid a backbone.a degradation protocol using de-o-acylation and subsequent alkaline de-n-acylation was applied to the lipopolysaccharide of ochrobactrum anthropi rough strain lmg 3301. three main oligosaccharide bisphosphates containing core-lipid a backbone structures were obtained after fractionation by anion-exchange hplc. using 1h and 13c nmr spectroscopy, including two-dimensional cosy, tocsy, and noe spectroscopy (roesy and noesy), the following structures were established: [formula: see text] where kdo i ...19989691452
evaluation of the relatedness of brucella spp. and ochrobactrum anthropi and description of ochrobactrum intermedium sp. nov., a new species with a closer relationship to brucella spp.the relatedness of brucella spp. and ochrobactrum anthropi was studied by protein profiling, western blot, immunoelectrophoresis and 16s rrna analysis. whole-cell and soluble proteins of brucellae and o. anthropi showed serological cross-reactivities quantitatively and qualitatively more intense than those existing with similar extracts of agrobacterium spp. numerical analysis of western blot profiles of whole-cell extracts showed that o. anthropi lmg 3301 was closer to brucella spp. than to o. ...19989734029
development of pcr primer systems for amplification of nitrite reductase genes (nirk and nirs) to detect denitrifying bacteria in environmental samples.a system was developed for the detection of denitrifying bacteria by the amplification of specific nitrite reductase gene fragments with pcr. primer sequences were found for the amplification of fragments from both nitrite reductase genes (nirk and nirs) after comparative sequence analysis. whenever amplification was tried with these primers, the known nir type of denitrifying laboratory cultures could be confirmed. likewise, the method allowed a determination of the nir type of five laboratory ...19989758798
molecular diversity of rhizobia occurring on native shrubby legumes in southeastern australiathe structure of rhizobial communities nodulating native shrubby legumes in open eucalypt forest of southeastern australia was investigated by a molecular approach. twenty-one genomic species were characterized by small-subunit ribosomal dna pcr-restriction fragment length polymorphism and phylogenetic analyses, among 745 rhizobial strains isolated from nodules sampled on 32 different legume host species at 12 sites. among these rhizobial genomic species, 16 belonged to the bradyrhizobium subgro ...19989758831
degradation of various chlorophenols under alkaline conditions by gram-negative bacteria closely related to ochrobactrum anthropi.from concrete debris of a demolished herbicide production plant several gram-negative bacterial strains were isolated, which exhibit metabolic capabilities for the degradation of 2,4-dichlorophenol (dcp)l), 4-chloro-2-methylphenol (mcp) and 4-chlorophenol (4-cp), while 2-chlorophenol (2-cp) was degraded at a slower rate. degradative activity was inducible and was impeded by adding of 100 mg/l of chloramphenicol to growing cultures. the strains displayed alkaliphilic properties with optimum dcp/m ...19989791948
molecular cloning, expression and site-directed mutagenesis of glutathione s-transferase from ochrobactrum anthropi.the gene coding for a novel glutathione s-transferase (gst) has been isolated from the bacterium ochrobactrum anthropi. a pcr fragment of 230 bp was obtained using oligonucleotide primers deduced from n-terminal and 'internal' sequences of the purified enzyme. the gene was obtained by screening of a genomic dna partial library from o. anthropi constructed in pbluescript with a pcr fragment probe. the gene encodes a protein (oagst) of 201 amino acids with a calculated molecular mass of 21738 da. ...19989794797
detection of ralstonia solanacearum, which causes brown rot of potato, by fluorescent in situ hybridization with 23s rrna-targeted probes.during the past few years, ralstonia (pseudomonas) solanacearum race 3, biovar 2, was repeatedly found in potatoes in western europe. to detect this bacterium in potato tissue samples, we developed a method based on fluorescent in situ hybridization (fish). the nearly complete genes encoding 23s rrna of five r. solanacearum strains and one ralstonia pickettii strain were pcr amplified, sequenced, and analyzed by sequence alignment. this resulted in the construction of an unrooted tree and suppor ...19989797321
[ochrobactrum anthropi bacteremia in a patient with aids]. 19989810554
ochrobactrum intermedium infection after liver transplantation.a case of bacteremia due to ochrobactrum intermedium, with concomitant liver abscesses, in an orthotopic liver transplant recipient is presented. identical microorganisms were isolated from fecal specimens and from an aspirate of a liver abscess that was indicative of invasion of the graft by gastrointestinal spread. 16s dna sequence analysis of the blood isolate revealed the recovery of the recently proposed new species o. intermedium, closely related to ochrobactrum anthropi and brucella spp.19999854103
ochrobactrum anthropi bacteremia.ochrobactrum anthropi (o. anthropi ), formerly known as achromobacter cdc group vd, is a gram-negative bacillus that is aerobic, oxidase producing, and nonlactose fermenting. this organism has been found in environmental and hospital water sources and has pathogenic potential in humans. most reports in the literature of o. anthropi bacteremia are associated with intravenous line infections. we describe a case of bacteremia with o. anthropi in a 33-month-old boy with acute osteomyelitis. o. anthr ...199910076115
crystallization and preliminary x-ray analysis of a new l-aminopeptidase-d-amidase/d-esterase activated by a gly-ser peptide bond hydrolysis.ochrobactrum anthropi possesses an l-aminopeptidase (dmpa) also able to act as a d-amidase/d-esterase. dmpa (40 kda) is activated by auto-catalyzed protein splicing liberating an alpha-amino group presumably used as a general base in the catalytic mechanism. two crystal forms were obtained at 294 k in 13-16% peg 2000 mono-methylether at ph 9.0, adding either 0.2 m magnesium chloride or 1 m lithium chloride. crystals of the first form belong to the space group c2221 and diffract to 3.0 a resoluti ...199910089474
ochrobactrum anthropi as an agent of nosocomial septicemia in the setting of aids. 199910194107
pcr assay for diagnosis of human brucellosis. 199910328689
ochrobactrum anthropi meningitis in a pre-term neonate. 199910342661
the dmpa aminopeptidase from ochrobactrum anthropi lmg7991 is the prototype of a new terminal nucleophile hydrolase family.the dmpa (d-aminopeptidase a) protein produced by ochrobactrum anthropi hydrolyses p-nitroanilide derivatives of glycine and d-alanine more efficiently than that of l-alanine. when regular peptides are utilized as substrates, the enzyme behaves as an aminopeptidase with a preference for n-terminal residues in an l configuration, thus exemplifying an interesting case of stereospecificity reversal. the best-hydrolysed substrate is l-ala-gly-gly, but tetra- and penta-peptides are also efficiently h ...199910377256
two new aminopeptidases from ochrobactrum anthropi active on d-alanyl-p-nitroanilide.two new enzymes which hydrolyse d-alanyl-p-nitroanilide have been detected in ochrobactrum anthropi lmg7991 extracts. the first enzyme, dmpb, was purified to homogeneity and found to be homologous to the dap protein produced by o. anthropi scrc c1-38 (atcc49237). the second enzyme, dmpa, exhibits a similar substrate profile when tested on p-nitroanilide derivatives of glycine and l/d-alanine, but the amounts produced by the ochrobactrum strain were not sufficient to allow complete purification. ...199910379365
brucella outer membrane lipoproteins share antigenic determinants with bacteria of the family rhizobiaceae.brucellae have been reported to be phylogenetically related to bacteria of the family rhizobiaceae. in the present study, we used a panel of monoclonal antibodies (mabs) to brucella outer membrane proteins (omps) to determine the presence of common omp epitopes in some representative bacteria of this family, i.e., ochrobactrum anthropi, phyllobacterium rubiacearum, rhizobium leguminosarum, and agrobacterium tumefaciens, and also in bacteria reported to serologically cross-react with brucella, i. ...199910391877
ochrobactrum anthropi endophthalmitis after vitreous surgery. 199910434878
ochrobactrum anthropi bacteremia associated with a catheter in an immunocompromised child and review of the pediatric literature. 199910440451
identification of an is711 element interrupting the wboa gene of brucella abortus vaccine strain rb51 and a pcr assay to distinguish strain rb51 from other brucella species and strains.brucella abortus vaccine strain rb51 is a natural stable attenuated rough mutant derived from the virulent strain 2308. the genetic mutations that are responsible for the roughness and the attenuation of strain rb51 have not been identified until now. also, except for an assay based on pulsed-field gel electrophoresis, no other simple method to differentiate strain rb51 from its parent strain 2308 is available. in the present study, we demonstrate that the wboa gene encoding a glycosyltransferas ...199910473532
cross-species characterisation of abundantly expressed ochrobactrum anthropi gene products.the identity of 45 protein spots representing 32 orthologues within the ochrobactrum anthropi proteome within a gradient of ph 4-7, and mass range 5-90 kda were determined across species boundaries. these proteins could be classified into 13 functional categories and establish metabolic, regulatory and translatory systems including amino acid biosynthesis, electron transport and the potential for plant symbiosis in a molecularly understudied organism. amino acid composition and/or peptide mass f ...199910493124
microbial urea-formaldehyde degradation involves a new enzyme, methylenediurease.the enzymic mechanism of metabolization of urea-formaldehyde condensation products (methyleneureas; mu) and the fate of the degradation products ammonium, urea and formaldehyde were studied in bacteria isolated from garden soil, which were able to use methyleneureas as the sole source of nitrogen for growth. an organism identified as ochrobactrum anthropi completely degraded methylenediurea (mdu) and dimethylenetriurea (dmtu) to urea, ammonia, formaldehyde and carbon dioxide. an enzyme designate ...199810526991
endotoxins and igg antibodies as indicators of occupational exposure to the microbial contaminants of metal-working fluids.the aim of this study was to evaluate workers' exposure to microbes and bacterial endotoxins during the use of metal-working fluids (mwf).199910541909
physical map and gene survey of the ochrobactrum anthropi genome using bacterial artificial chromosome contigs.bacterial artificial chromosome (bac) clones are effective mapping and sequencing reagents for use with a wide variety of small and large genomes. this report describes research aimed at determining the genome structure of ochrobactrum anthropi, an opportunistic human pathogen that has potential applications in biodegradation of hazardous organic compounds. a bac library for o. anthropi was constructed that provides a 70-fold genome coverage based on an estimated genome size of 4.8 mb. the libra ...199910587947
an algorithm for the assembly of robust physical maps based on a combination of multi-level hybridization data and fingerprinting data.we have developed an algorithm which combines data obtained from restriction digestion experiments and hybridization experiments to construct robust physical maps of whole chromosomes. the algorithm has been incorporated into a program which accepts hybridization data consisting of an unordered hybridization matrix and fingerprinting data containing band coordinates for each clone. the combined data is used to produce a non-redundant, ordered matrix which can be further reduced to represent a mi ...199910627143
a study on the prevalence of gram-negative bacteria in bulk tank milk.bulk tank milk from 131 dairy herds in eastern south dakota and western minnesota were examined for coliforms and noncoliform bacteria. coliforms were detected in 62.3% of bulk tank milk samples. counts ranged from 0 to 4.7 log10 cfu/ml. the mean count was 3.4 log10 cfu/ml. gram-negative noncoliform bacteria were observed in 76.3% of bulk tank milk. counts ranged from 0 to 6.2 log10 cfu/ml. the mean count was 4.8 log10 cfu/ml. a total of 234 isolates from bulk tank milk were examined to species ...199910629809
a new variant of the ntn hydrolase fold revealed by the crystal structure of l-aminopeptidase d-ala-esterase/amidase from ochrobactrum anthropi.the l-aminopeptidase d-ala-esterase/amidase from ochrobactrum anthropi (dmpa) releases the n-terminal l and/or d-ala residues from peptide substrates. this is the only known enzyme to liberate n-terminal amino acids with both d and l stereospecificity. the dmpa active form is an alphabeta heterodimer, which results from a putative autocatalytic cleavage of an inactive precursor polypeptide.200010673442
modulation of the glutathione s-transferase in ochrobactrum anthropi: function of xenobiotic substrates and other forms of stress.the gluthathione s-transferase gene of the atrazine-degrading bacterium ochrobactrum anthropi (oagst) encodes a single-subunit polypeptide of 201 amino acid residues (favaloro et al. 1998, biochem. j. 335, 573-579). rna blot analysis showed that the gene is transcribed into an mrna of about 800 nucleotides, indicating a monocistronic transcription of the oagst gene. the modulation of oagst in this bacterium, in the presence of different stimulants, was investigated. the level of expression of oa ...200010677378
evaluation of autoscan-w/a and the vitek gni+ automicrobic system for identification of non-glucose-fermenting gram-negative bacilli.the autoscan-w/a (w/a; dade behring microscan inc., west sacramento, calif.) and vitek automicrobic system (vitek ams; biomérieux vitek systems, inc., hazelwood, mo.) are both fully automated microbiology systems. we evaluated the accuracy of these two systems in identifying nonglucose-fermenting gram-negative bacilli. we used the w/a with conventional-panel neg combo type 12 and vitek gni+ identification systems. a total of 301 isolates from 25 different species were tested. of these, 299 isola ...200010699007
epidemiological investigation of ochrobactrum anthropi strains isolated from a haematology unit.ochrobactrum anthropi is an oxidase-producing gram-negative bacillus preferring aqueous environments. it is an opportunist of low pathogenicity with a wide and unpredictable antibiotic resistance. we observed bacteraemia caused by this organism in two immunocompromized patients hospitalized in the same haematology unit and catheter-associated sepsis was recognized within two days. another isolate was obtained from the stools of a third patient of the same unit. environmental investigations recov ...200010706799
isolation of bacteria other than helicobacter pylori from stomachs of squirrel monkeys (saimiri spp.) with gastritis.gastric biopsy specimens obtained from 12 squirrel monkeys (saimiri spp.) were investigated by culture for the presence of bacteria. the stomachs of two monkeys with gastritis were colonized with gram-negative, urease-positive bacteria, identified as ochrobactrum anthropi by the vitek and api nft methods (biomérieux). a third monkey with gastritis was positive for aeromonas salmonicida and pseudomonas vesicularis (both urease-negative). no helicobacter pylori was isolated from squirrel monkeys. ...200010711437
gene cloning, nucleotide sequencing, and purification and characterization of the d-stereospecific amino-acid amidase from ochrobactrum anthropi sv3.the gene encoding the d-stereospecific amino-acid amidase from ochrobactrum anthropi sv3 was cloned and sequenced. analysis of 7.3 kb of genomic dna revealed the presence of six orfs, one of which (daaa) encodes the d-amino-acid amidase. this enzyme, daaa, is composed of 363 amino-acid residues (molecular mass 40 082 da), and the deduced amino-acid sequence exhibits homology to alkaline d-peptidase from bacillus cereus df4-b (32% identity), dd-peptidase from streptomyces r61 (29% identity), and ...200010727942
occurrence of natural dixenic associations between the symbiont photorhabdus luminescens and bacteria related to ochrobactrum spp. in tropical entomopathogenic heterorhabditis spp. (nematoda, rhabditida).bacteria naturally associated with the symbiont photorhabdus luminescens subsp. akhurstii were isolated from the entomopathogenic nematode heterorhabditis indica. bacterial isolates distinct from p. luminescens subsp. akhurstii were obtained from 33% of the samples. fourteen bacterial isolates, from nematodes collected from three different caribbean islands, were characterized by conventional phenotypic tests, restriction fragment length polymorphism and sequence analyses of pcr-amplified 16s rr ...200010746775
characterization of heat, oxidative, and acid stress responses in brucella melitensis.brucella melitensis is a facultative intracellular pathogen which is able to survive and replicate within phagocytic cells. therefore, it has to adapt to a range of different hostile environments. in order to understand the mechanisms of intracellular survival employed by virulent b. melitensis 16m, an initial approach consisting of analysis of the differences in patterns of protein synthesis in response to heat, oxidative, and acid ph stresses by two-dimensional (2-d) polyacrylamide gel electro ...200010768994
a novel amidase (half-amidase) for half-amide hydrolysis involved in the bacterial metabolism of cyclic imides.a novel amidase involved in bacterial cyclic imide metabolism was purified from blastobacter sp. strain a17p-4. the enzyme physiologically functions in the second step of cyclic imide degradation, i.e., the hydrolysis of monoamidated dicarboxylates (half-amides) to dicarboxylates and ammonia. enzyme production was enhanced by cyclic imides such as succinimide and glutarimide but not by amide compounds which are conventional substrates and inducers of known amidases. the purified amidase showed h ...200010788365
brucella abortus and its closest phylogenetic relative, ochrobactrum spp., differ in outer membrane permeability and cationic peptide resistance.the outer membrane (om) of the intracellular parasite brucella abortus is permeable to hydrophobic probes and resistant to destabilization by polycationic peptides and edta. the significance of these unusual properties was investigated in a comparative study with the opportunistic pathogens of the genus ochrobactrum, the closest known brucella relative. ochrobactrum spp. oms were impermeable to hydrophobic probes and sensitive to polymyxin b but resistant to edta. these properties were traced to ...200010816465
peritonitis due to ochrobactrum anthropi in a patient undergoing continuous ambulatory peritoneal dialysis. 200010841106
ochrobactrum anthropi bacteremia in a patient on hemodialysis.although newer tunneled dialysis catheters offer improved capacity for blood flow and efficiency of dialysis, catheter-associated bacteremia remains an extremely important complication of this access strategy. this is a report of a case of catheter-associated bacteremia with ochrobactrum anthropi, a water-borne gram-negative rod with an unusual pattern of antibiotic resistance. given the organism's hydrophilic property and the frequency of catheter use in debilitated individuals with end-stage r ...200010845846
detection of ralstonia solanacearum strains with a quantitative, multiplex, real-time, fluorogenic pcr (taqman) assay.a fluorogenic (taqman) pcr assay was developed to detect ralstonia solanacearum strains. two fluorogenic probes were utilized in a multiplex reaction; one broad-range probe (rs) detected all biovars of r. solanacearum, and a second more specific probe (b2) detected only biovar 2a. amplification of the target was measured by the 5' nuclease activity of taq dna polymerase on each probe, resulting in emission of fluorescence. taqman pcr was performed with dna extracted from 42 r. solanacearum and g ...200010877778
fluorescent whole-cell hybridization with 16s rrna-targeted oligonucleotide probes to identify brucella spp. by flow cytometry.a whole-cell hybridization assay with fluorescent oligonucleotide probes derived from the 16s rrna sequence of brucella abortus in combination with flow cytometry has been developed. with the three fluorescent probes selected, a positive signal was observed with all the representative strains of the species and biovars of brucella and with a total of nine different brucella clinical isolates. using the b9 probe in the hybridization assay, it was possible to discriminate between brucella suis bio ...200010878084
infective endocarditis and septic embolization with ochrobactrum anthropi: case report and review of literature.ochrobactrum anthropi, previously known as cdc group vd, is an aerobic, gram-negative bacillus of low virulence that occasionally causes human infection. we describe a case of infective endocarditis with o. anthropi complicated by septic embolization. a review of all the literature reported cases of o. anthropi infection is presented and categorized into 'central line related', 'transplant related' and "other pyogenic infections". mortality appears to be related to the underlying disease state, ...200010908026
ochrobactrum anthropi peritonitis in a capd patient. 200010908034
isolation and characterization of diverse halobenzoate-degrading denitrifying bacteria from soils and sediments.denitrifying bacteria capable of degrading halobenzoates were isolated from various geographical and ecological sites. the strains were isolated after initial enrichment on one of the monofluoro-, monochloro-, or monobromo-benzoate isomers with nitrate as an electron acceptor, yielding a total of 33 strains isolated from the different halobenzoate-utilizing enrichment cultures. each isolate could grow on the selected halobenzoate with nitrate as the terminal electron acceptor. the isolates obtai ...200010919805
crystal structure of a d-aminopeptidase from ochrobactrum anthropi, a new member of the 'penicillin-recognizing enzyme' family.beta-lactam compounds are the most widely used antibiotics. they inactivate bacterial dd-transpeptidases, also called penicillin-binding proteins (pbps), involved in cell-wall biosynthesis. the most common bacterial resistance mechanism against beta-lactam compounds is the synthesis of beta-lactamases that hydrolyse beta-lactam rings. these enzymes are believed to have evolved from cell-wall dd-peptidases. understanding the biochemical and mechanistic features of the beta-lactam targets is cruci ...200010986464
diversity and contribution of the intestinal bacterial community to the development of musca domestica (diptera: muscidae) larvae.the bacterial diversity in the intestinal tract of musca domestica l. was examined in larvae collected from turkey bedding and corn silage. aerobic culturing yielded 25 bacterial species, including 11 from larvae collected from turkey bedding and 14 from larvae collected from corn silage. providencia rettgeri (hadley, elkins & caldwell) was the only species common to both environments. two mammalian pathogens, yersinia pseudotuberculosis (pfeiffer) and ochrobactrum anthropi (holmes), were isolat ...200011126551
limited genetic diversity of brucella spp.multilocus enzyme electrophoresis (mlee) of 99 brucella isolates, including the type strains from all recognized species, revealed a very limited genetic diversity and supports the proposal of a monospecific genus. in mlee-derived dendrograms, brucella abortus and a marine brucella sp. grouped into a single electrophoretic type related to brucella neotomae and brucella ovis. brucella suis and brucella canis formed another cluster linked to brucella melitensis and related to rhizobium tropici. th ...200111136777
taxonomic characterization of ochrobactrum sp. isolates from soil samples and wheat roots, and description of ochrobactrum tritici sp. nov. and ochrobactrum grignonense sp. nov.a large collection of bacterial strains, immunotrapped from soil and from the wheat rhizoplane, was subjected to polyphasic taxonomy by examining various pheno- and genotypic parameters. strains were grouped on (inter) repetitive extragenic palindromic dna (rep) pcr profiles at the intraspecies level. pheno- and genotypic characters were assessed for representatives from 13 different rep groups. strains of nine rep groups constituting two physiological biolog clusters fell in the coherent dna-dn ...200011155998
clinical significance and taxonomy of actinobacillus hominis.clinical findings in 36 immunosuppressed patients with lower respiratory tract infection or bacteremia with actinobacillus hominis are described. animal contact was only recorded for three patients; nine patients died despite appropriate antimicrobial treatment. although infections with this microorganism seem to be rare, the fact that 37 of 46 strains characterized in this study have been found in copenhagen indicates that under-reporting may occur. a. hominis is phenotypically relatively homog ...200111230406
purification, characterization, and gene cloning of purine nucleosidase from ochrobactrum anthropi.a bacterium, ochrobactrum anthropi, produced a large amount of a nucleosidase when cultivated with purine nucleosides. the nucleosidase was purified to homogeneity. the enzyme has a molecular weight of about 170,000 and consists of four identical subunits. it specifically catalyzes the irreversible n-riboside hydrolysis of purine nucleosides, the k(m) values being 11.8 to 56.3 microm. the optimal activity temperature and ph were 50 degrees c and ph 4.5 to 6.5, respectively. pyrimidine nucleoside ...200111282633
serum is the preferred clinical specimen for diagnosis of human brucellosis by pcr.human brucellosis poses a significant public health problem in many developing countries and requires fast and accurate diagnosis. a pcr assay amplifying part of the 31-kda brucella abortus antigenic protein gene sequence was developed and applied to whole-blood and serum samples from 31 brucellosis patients and 45 healthy individuals. all patients except one had detectable brucella dna in either whole blood or serum (combined sensitivity, 97%), but the assay sensitivity was higher with serum sa ...200111283112
16s ribosomal dna characterization of nitrogen-fixing bacteria isolated from banana (musa spp.) and pineapple (ananas comosus (l.) merril).nitrogen-fixing bacteria isolated from banana (musa spp.) and pineapple (ananas comosus (l.) merril) were characterized by amplified 16s ribosomal dna restriction analysis and 16s rrna sequence analysis. herbaspirillum seropedicae, herbaspirillum rubrisubalbicans, burkholderia brasilensis, and burkholderia tropicalis were identified. eight other types were placed in close proximity to these genera and other alpha and beta proteobacteria.200111319127
evaluating genome dynamics: the constraints on rearrangements within bacterial genomes.inversions and translocations distinguish the genomes of closely related bacterial species, but most of these rearrangements preserve the relationship between the rearranged fragments and the axis of chromosome replication. within species, such rearrangements are found less frequently, except in the case of clinical isolates of human pathogens, where rearrangements are very frequent.200011380986
characterization, cloning and sequence analysis of the inducible ochrobactrum anthropi ampc beta-lactamase.ochrobactrum anthropi is resistant to most cephalosporins and penicillins due, at least in part, to the inducible expression of a single beta-lactamase. the beta-lactamase gene has been cloned and sequenced. it encodes an ampc-type class 1 serine active-site enzyme that hydrolyses mainly cephalosporins and is resistant to inhibition by clavulanic acid. expression of the ampc gene is inducible via a typical ampr regulator, which is encoded upstream of ampc. inducible expression is retained follow ...200111389106
chronic postoperative endophthalmitis secondary to ochrobactrum anthropi. 200111421027
production of bartonella genus-specific monoclonal antibodies.monoclonal antibodies (mabs) which react with heat-resistant proteins with molecular masses of 32 to 33 kda of 14 different bartonella species were produced. these antibodies did not react with antigens of 26 diverse bacterial strains by microimmunofluorescence assay except mab b3d4, which reacted with chlamydia psittaci and chlamydia trachomatis at low titers. the identification of a common bartonella antigenic protein will make it possible to later produce a diagnostic antigen by cloning and e ...200111427441
resistance to antibiotics and biocides among non-fermenting gram-negative investigate the antibiotic and biocide susceptibilities of clinical isolates of rarely encountered gram-negative, non-fermenting bacteria.200111442564
molecular characterization of chromosomal class c beta-lactamase and its regulatory gene in ochrobactrum anthropi.ochrobactrum anthropi, formerly known as cdc group vd, is an oxidase-producing, gram-negative, obligately aerobic, non-lactose-fermenting bacillus of low virulence that occasionally causes human infections. it is highly resistant to all beta-lactams except imipenem. a clinical isolate, slo74, and six reference strains were tested. mics of penicillins, aztreonam, and most cephalosporins tested, including cefotaxime and ceftazidime, were >128 microg/ml and of cefepime were 64 to >128 microg/ml. cl ...200111451692
an ochrobactrum anthropi gene conferring paraquat resistance to the heterologous host escherichia coli.a new gene, pqra, conferring paraquat resistance to the heterologous host escherichia coli, from a chromosomal dna library of ochrobactrum anthropi jw2, was cloned and analyzed. cells of e. coli transformed with a plasmid carrying the pqra gene showed elevated resistance to paraquat, but not to hydrogen peroxide. the predicted amino acid sequence of the pqra polypeptide showed 71% identity with mll7495 hypothetical membrane protein in mesorhizobium loti, 49% identity with pa2269 protein in pseud ...200111467833
a marker-dense physical map of the bradyrhizobium japonicum genome.bacterial artificial chromosome (bac) clones are effective mapping and sequencing reagents for use with a wide variety of small and large genomes. this report describes the development of a physical framework for the genome of bradyrhizobium japonicum, the nitrogen-fixing symbiont of soybean. a bac library for b. japonicum was constructed that provides a 77-fold genome coverage based on an estimated genome size of 8.7 mb. the library contains 4608 clones with an average insert size of 146 kb. to ...200111483585
identification of strains of alcaligenes and agrobacterium by a polyphasic approach.the number of stable discriminant biochemical characters is limited in the genera alcaligenes and agrobacterium, whose species are consequently difficult to distinguish from one another by conventional tests. moreover, genomic studies have recently drastically modified the nomenclature of these genera; for example, alcaligenes xylosoxidans was transferred to the genus achromobacter in 1998. twenty-five strains of achromobacter xylosoxidans, three strains of an agrobacterium sp., five strains of ...200111526136
the role of microbial biofilms in deterioration of space station candidate materials.formation of microbial biofilms on surfaces of a wide range of materials being considered as candidates for use on the international space station was investigated. the materials included a fibre-reinforced polymeric composite, an adhesive sealant, a polyimide insulation foam, teflon cable insulation, titanium, and an aliphatic polyurethane coating. they were exposed to a natural mixed population of bacteria under controlled conditions of temperature and relative humidity (rh). biofilms formed o ...199811541393
cloning, nucleotide sequence, and expression of the brucella melitensis sucb gene coding for an immunogenic dihydrolipoamide succinyltransferase homologous protein.the brucella melitensis sucb gene encoding the dihydrolipoamide succinyltransferase (e2o) enzyme (previously identified as an immunogenic protein in infected sheep) was cloned and sequenced. the amino acid sequence predicted from the cloned gene revealed 88.8 and 51.2% identity to the dihydrolipoamide succinyltransferase sucb protein from brucella abortus and escherichia coli, respectively. sera from naturally infected sheep showed antibody reactivity against the recombinant sucb protein.200111553602
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