| the effect of bordetella pertussis on the antibody response in mice to type iii pneumococcal polysaccharide. | the effect of an i.p. injection of bordetella pertussis on the primary humoral immune response in mice to the thymus-independent antigen siii has been studied. suppression of the antibody response occurred when pertussis cells were injected at the same time as an optimal immunizing dose of siii. in contrast, the antibody response to high doses of siii was enhanced by b. pertussis. when siii alone was injected, only 19s antibody was detected. however, when b. pertussis was administered with eithe ... | 1976 | 3610 |
| the influence of growth medium on the interactions between bordetella pertussis and staphylococcus aureus. | previous observers showed that many strains of staphylococcus aureus stimulated the growth of bordetella pertussis but we have found the reverse: the growth of all available strains of b. pertussis on charcoal-agar medium was inhibited by a standard strain of s. aureus; and 17 of 18 strains of s. aureus (as well as several other organisms) inhibited the growth of a standard strain of b. pertussis. all inhibiting colonies had an unusual brown colouration on the charcoal agar used in the investiga ... | 1976 | 6798 |
| histamine hypersensitivity in mice induced by bordetella pertussis or pharmacologic beta adrenergic blockade. effects of adrenergic, cholinergic, and other drugs. | the effects of prostaglandin e1, e2, f2alpha (pge2 pgf2alpha), isoproterenol, epinephrine, norepinephrine, salbutamol, practolol, atropine, aminophylline, and corticosterone on the hypersensitivity to anaphylaxis, histamine, and serotonin in bordetella pertussis-treated mice and propranolol-treated mice were investigated. female hla-sw (icr) mice, 27-29 gm, were injected with pertussis vaccine intravenously 4 days before challenge with antigen, histamine, or serotonin. alternatively, instead of ... | 1976 | 9437 |
| biologically active components and properties of bordetella pertussis. | | 1976 | 11644 |
| the effects of bordetella pertussis vaccine on cerebral vascular permeability. | the effect of bordetella pertussis vaccine on the cerebral vascular permeability in the mouse was studied by a radio-isotope method (131i-labelled hsa). intravenous injection of 4 x 1010 heat-killed pertussis organisms caused a measurable increase in permeability in normal mice. cryoinjury to the cerebral hemispheres resulted in a striking increase in vascular permeability at 24 h. this declined within 48 h and stabilized at a level fractionally higher than normal at 7 days ("healed lesion"). wh ... | 1976 | 12780 |
| adenyl cyclase in bordetella pertussis vaccines. | brodetella pertussis organisms have a large amount of extracytoplasmic adenyl cyclase, part of which is found in the supernatant culture medium during exponential growth. the enzyme differs from previously studied bacterial adenyl cyclases in biochemical characteristics as well as in location. several commercial pertussis vaccines were found to contain adenyl cyclase activity; this activity is probably due to the extracytoplasmic enzyme associated with and released from the whole cells in the va ... | 1977 | 19542 |
| the insulin-like action of bordetella pertussis vaccine in rats. | treatment of rats with bordetella pertussis vaccine significantly lowered the blood sugar level 4 days later but the vaccine did not alter the level in diabetic rats. the vaccine, like insulin, raised glycogen levels in liver, skeletal muscle and heart and reduced the plasma free fatty acid concentration. the action of a beta-adrenoceptor blocker was potentiated by the vaccine but not by insulin. part of the hypoglycaemic action of the vaccine is probably due to beta-adrenoceptor blockade. | 1977 | 22497 |
| action of chloroquine on pleurisy due to bordetella pertussis hypersensitivity. | | 1978 | 29110 |
| subunit structure of islets-activating protein (iap), a new protein isolated from the culture media of bordetella pertussis. | the subunit structure was studied of islets-activating protein (iap), a new protein recently isolated from the culture media of bordetella pertussis and possessing a unique action, i.e., potentiating insulin secretory responses of animals, iap dissociated into three subunits, f-1, f-2, and f-3, when incubated in 8m urea. three subunits isolated by chromatography on cm-sepharose and deae-sepharose columns showed different molecular weights (f-1: 44,000, f-2: 20,000, f-3: 11,000) and different iso ... | 1978 | 29892 |
| viruses and bacteria associated with acute respiratory illnesses in young children in general practice. | the results obtained and the laboratory methods used for the isolation of viruses and bacteria from malaysian children with acute respiratory illnesses seen in a private clinic are described. of the 65 children studied virus isolations were obtained from 26 children, bacteria from 10 and both virus and bacteria from another 5. the agents isolated were influenza viruses, parainfluenza viruses, adenoviruses, bordetella pertussis, streptococcus pneumoniae, haemophilus influenzae and staphylococcus ... | 1978 | 30173 |
| bordetella pertussis does not induce beta-adrenergic blockade. | bordetella pertussis organisms induce histamine sensitivity and diminish the normal hyperglycemic response to epinephrine in experimental animals. these effects have been attributed to beta-adrenergic blockade. however, under conditions in which the decrease in epinephrine-induced hyperglycemia after b. pertussis administration was demonstrable, there was no change in rat reticulocyte beta-adrenergic receptor number or affinity measured by iodohydroxybenzylpindolol binding or in isoproterenol-st ... | 1978 | 32138 |
| purification and crystallization of fimbrial hemagglutinin from bordetella pertussis. | a procedure to purify and crystallize fimbrial hemagglutinin from bordetella pertussis is described. redissolved crystals had the same specific activity as the original, purified solution of fimbrial hemagglutinin. about 97% of the weight of washed crystals was accounted for by amino acids. | 1979 | 39033 |
| fimbrial hemagglutinin in stationary and shake cultures of bordetella pertussis. | bordetella pertussis produced hemagglutinin in stationary cultures; in cultures kept under constant shaking, hemagglutinin was found only during the first 48 h of incubation but not after 3 to 5 days. the type of medium had a pronounced effect on production of hemagglutinin. strain differences in ability to produce hemagglutinin were also detected. | 1979 | 39897 |
| polymorphonuclear leukocyte-inhibitory factor of bordetella pertussis. ii. localization in the outer membrane. | the outer and inner membranes and cytoplasm of spheroplasts of a strain of phase i b. pertussis were fractionated by density gradient centrifugation. the high density vesicles of the outer membranes isolated had the "pili" characteristic of the bacteria and the same antigenicty as the bacterial surface. activities for inhibition of polymorphonuclear leukocytes were also almost exclusively localized in this outer membrane fraction. the histamine-sensitizing activity was more dispersed, but its sp ... | 1979 | 43725 |
| immunization against nippostrongylus brasiliensis in the rat. a study on the use of antigen extracted from adult parasites and the parameters which influence the level of protection. | it was found that protective immunity in excess of 90% reduction in worm burden could be stimulated against nippostrongylus brasiliensis in rats by using an extract of adult nippostrongylus worms. the level of protection achieved was influenced by several factors. thus, the use of bordetella pertussis as adjuvant significantly increased the level of protection which, in addition, was shown to be influenced by the amount of worm antigen used. furthermore, antigen administered in multiple doses wa ... | 1979 | 44095 |
| non-specific induction of increased resistance in mice to trypanosoma congolense and trypanosoma brucei by immunostimulants. | administration of the immunostimulants corynebacterium parvum, bacillus calmette-guérin (bcg) or bordetella pertussis prior to, or at the same time as, challenge with trypanosoma congolense significantly increased survival times in mice, both of trypano-susceptible (a/j) and trypano-resistant (c57bl) strains. the increased survival time was associated with significant alterations in parasitaemia, which included lengthening of the pre-patent period, a delay in the time taken to reach the first pe ... | 1979 | 44360 |
| spontaneous and methylcholanthrene-enhanced thyroiditis in buf rats. ii. induction of experimental autoimmune thyroiditis without completed freund's adjuvant. | the immunization of buffalo strain (buf) rats with rat thyroid extract and bordetella pertussis vaccine without complete freund's adjuvant resulted in experimental autoimmune thyroiditis (eat) in 90% of the rats by the 10th day after immunization. the treatment did not elicit thyroiditis in lewis strain (lew) rats. this susceptibility to the induction of eat without the use of complete freund's adjuvant was inherited by f1 hybrids of buf and lew. neither histamine sensitizing factor from b. pert ... | 1975 | 46238 |
| immunologic and biochemical properties of the histamine-sensitizing factor from bordetella pertussis. | a highly potent extract of the histamine sensitizing factor (hsf) of bordetella pertussis was isolated by extraction of bacterial cells with urea buffer and subsequent gel filtration. this preparation of hsf also contained leukocytosis-promoting activity and adjuvant activity for reaginic and hemagglutinating antibodiesl digestion of this extract with pronase or trypsin partially destroyed histamine-sensitizing activity, leukocytosis-promoting activity, and adjuvant activity for reaginic antibod ... | 1975 | 46244 |
| letter: effect of bordetella pertussis vaccine on growth of hepatoma. | | 1975 | 46557 |
| a new method for the measurement of the opsonin titres of antisera to bordetella pertussis. | | 1975 | 47859 |
| taxonomic distribution of the antigen eliciting bactericidal antibody for bordetella pertussis. | strains of bordetella pertussis varied in their ability to elicit (in mice) an antibody bactericidal for an antiserum-sensitive strain of b. pertussis, although antibody was usually detectable after only one injection. high titres were produced by a course of seven injections with all strains of b. pertussis tested (six of phase i and three of phase iv) but not with three strains of other bordetella species nor with two unrelated organisms, a finding of possible taxonomic value. preliminary inve ... | 1975 | 49392 |
| use of pertussis vaccine in an epidemic involving hospital staff. | an epidemic of pertussis occurred among hospital staff caring for paediatric patients. eight physicians and five nurses were affected. pertussis developed in six newborn infants exposed to infected hospital staff in the nursery. erythromycin prophylaxis was used to control the epidemic. clinical pertussis developed in five adults infected with bordetella pertussis before erythromycin was used, whereas symptoms developed in only one of the eight adults who became infected after erythromycin proph ... | 1975 | 51354 |
| the antibody responses to myelin basic protein (bp) in lewis rats: the effects of bordetella pertussis. | a time-course study was made of the systemic humoral immune response of lewis rats to myelin basic protein (bp) as influenced by the dosage of ancillary pertussis adjuvant. peak activities were observed 5 to 7 weeks after injection. when injected proximal to bp and mycobacterium butyricum in complete freund's adjuvant (cfa), bordetella pertussis at the level of 4 billion organisms doubled the antibody-binding activity of rat sera for 125i-labeled bp as compared to activities obtained with 0, 2, ... | 1975 | 51876 |
| loss of protective antigen, histamine-sensitising factor and envelope polypeptides in cultural variants of bordetella pertussis. | five bordetella pertussis strains of phase i were grown in conventional casamino-acid medium and in media modified by adding high concentrations of mgso4 or nicotinic acid. cells grown in high-magnesium media (in the c-mode) had only about 4% of the protective antigen (pa) and 6% of the histamine-sensitising factor (hsf) of cells from the normal medium. envelopes from c-mode organisms when examined by sds-page showed a loss of 28k and 30k polypeptide bands. similar parallel losses of pa, hsf and ... | 1976 | 57240 |
| isolation and properties of the leukocytosis- and lymphocytosis-promoting factor of bordetella pertussis. | the leukocytosis- and lymphocytosis-promoting factor (lpf) of bordetella pertussis has been isolated to near homogeneity by physical, chemical, and electron microscopical criteria. lpf contains 14.5% nitrogen and is lipid and carbohydrate free. it is apparently composed of four polypeptide subunits. lpf caused leukocytosis and lymphocytosis in "nude" as well as in normal mice. in addition, purified lpf also induced histamine sensitization and hypoglycemia and refractoriness to the hyperglycemic ... | 1976 | 58054 |
| letter: protective activity of whooping-cough convalescent serum and serum-iga level in mice infected with bordetella pertussis. | | 1976 | 59233 |
| [vaccine from the cell fragments of bordetella pertussis. i. protective, sensitizing properties and morphological characteristics of the vaccine]. | the authors present the results of studying the protective and sensitizing properties of a new preparation made of a ultrasonic disintegrate of pertussis microbes treated by ethyl ether. as shown by electron microscopy, the preparation consisted of the cell wall elements (the membrane), remnants of the cytoplasm and protectosome, i.e. it represented a vaccine consisting of cell fragments. in crude and sorbed condition it possessed marked protective properties (a test on mice). the content of pro ... | 1976 | 64095 |
| rosette-forming mast cells in rat anaphylaxis. immunological characteristics of mast cell rosettes. | peritoneal mast cells from immunized rats can form rosettes with antigen-coated sheep red blood cells. the receptor responsible for this active rosette formation is shown to be ige cytophilic antibody: rosettes are inhibited by previous contact of mast cells with antigen, or with anti-ige antiserum; the kinetics of mast cell rosettes following a primary immunization with ovalbumin and bordetella pertussis vaccine is similar to the kinetics of reaginic antibody response. furthermore, a reaginic s ... | 1977 | 73505 |
| [protection against infection with bordetella pertussis by an oligopeptide from bordetella pertussis (author's transl)]. | we have isolated from bordetella pertussis an oligopeptide with characteristic amino acid composition. this peptide was applied to mice in standardized tests for pertussis immunization. in three tests with three independent isolates of peptide, a significant and dose dependent protection was observed. one microgram of peptide per mouse produces the same protective effect as 0.1 iu of pertussis vaccine. it is important to note that similar peptides can be isolated from other bacteria and other dn ... | 1978 | 77606 |
| partial purification and some properties of inhibitors to the hemagglutinating activity of leukocytosis-promoting factor (lpf) from bordetella pertussis [proceedings]. | | 1978 | 79665 |
| characteristics of the t-dependent alpha(1 leads to 6) glucosyl (dextran) antibody response induced in mice with isomaltohexaose coupled to chicken gamma-globulin. | isomaltohexaose flavazole coupled to chicken gamma-globulin (im6-cgg) induced t cell-dependent anti-alpha(1 leads to 6) dextran-specific igm and igg responses in cba, balb/c and a strain mice. the igg responses were of restricted heterogeneity as judged by isoelectric focusing, and belonged mostly to the igg1 subclass with a minor igg3 component in the case of balb/c and cba mice. all four subclasses of igg were produced in a strain mice. in contrast, native dextran b 512 induces exclusively t c ... | 1979 | 93549 |
| bordetella pertussis in a partially immunized host: case report. | | 1978 | 96376 |
| [immune response to tetanus toxoid in mice receiving protein deficient diet immediately after weaning]. | the humoral immune response to tetanus toxoid is evaluated by passive haemagglutination test in mice receiving low diet immediately after weaning during 15 and 30 days. the results show that the more deficient mice give the best antitoxin titers but after the challenge the antibody immune response become proportional to the protein restriction. in other respects and antitoxin titers are higher in 30 days restricted mice than in 15 days deficient ones. the effect of bordetella pertussis adjuvant ... | 1978 | 122306 |
| the effects of humoral, cellular and non-specific immunity on intracerebral bordetella pertussis infections in mice. | when mice were injected intracerebrally with doses of bordetella pertussis vaccine greater than 5 imd 50 and challenged intracerebrally 14 days later with virulent b. pertussis there was an immediate reduction in the numbers of organisms. an analysis of this in vivo bactericidal effect has shown that large doses of an unrelated vaccine, salmonella typhosa, equivalent in cell mass to about 50 imd 50 of b. pertussis vaccine can achieve this effect, so for such doses the effect must be partly non-s ... | 1975 | 163275 |
| factors influencing the stability of acid-precipitated polyvalent bordetella pertussis bulk suspensions. | according to the statistical analysis of the potency values of acid precipitated polyvalent bulk suspensions of bordetella pertussis, the potency of suspensions decreased below 8 iu/30 iou during storage for 5 to 9.8 years. the average annual decrease over a 13-year period was 2.03 iu/30 iou. the periodicity in the decrease of potency values was assumed to be connected with changes in the quality of the casein hydrolysate ingredient of the medium. periodic functions employed to approach potency ... | 1975 | 164759 |
| the use of bordetella pertussis preserved in liquid nitrogen as a challenge suspension in the kendrick mouse protection test. | | 1975 | 165201 |
| the use of spheroplast-derived strains to differentiate between bordetella pertussis heat-labile agglutinogens and protective antigen for mice. | | 1975 | 165202 |
| cell-envelope proteins of bordetella pertussis. | cell-envelope polypeptides of eight phase-i and five phase-iv strains of bordetella pertussis were compared by sds-polyacrylamide gel electrophoresis. all phase-i strains gave a strikingly similar but complex pattern of protein bands, which did not appear to vary with known differences in heat-labile agglutinogens. phase-iv strains gave the same pattern as phase-i strains, except that one band was missing and another was either much reduced or absent. envelopes from phase-i strains grown in horn ... | 1975 | 165297 |
| immunodeficiency in the chicken. i. disparity in suppression of antibody responses to various antigens following surgical bursectomy. | the extent of suppression of antibody response by bursectomy (bx) was examined as a measure of the seeding sequence of different clones from the bursa to peripheral lymphoid tissues. chickens were bursectomized surgically 1, 4 or 7 days after hatching and immunized later with four antigens: sheep red blood cells (srbc); bordetella pertussis (bp); human serum albumin (hsa); influenza virus (iv). the kinetics of the antibody responses were delayed in bursectomized birds when compared with the cont ... | 1975 | 166036 |
| effect of bordetella pertussis extract and vasoactive amines on vascular permeability. | vasxular permeability to evans blue dye and 131-i-labeled human serum albumin was studied in normal mice and in mice treated with alkaline saline extracts (se) from bordetella pertussis cells. skin sites inoculated intracutaneously with small doses of histamine, serotonin, or a combination of these 2 substances were more permeable in se-treated mice than in normal animals. intravenously administered catecholamines were able to reduce in varying degrees the vascular permeability induced by seroto ... | 1975 | 166102 |
| studies on the immunizing capacity of orally administered particulate antigens. i. the efficiency of killed bordetella pertussis cells. | the single i.p. injection of 2.5 times 10-8 killed b. pertussis cells protected 23 out of a group of 24 nmri mice (95.8%) against the subsequent intracerebral infection, whilst 13 out of 24 mice (54.2%) survived the intracerebral challenge with virulent b. pertussis cells after prior oral administration of 2.5 times 10-11 killed b. pertussis cells, as demonstrated by the mouse protection test. similar treatment with non-specific substances, such as egg white and saline, did not result in any inc ... | 1975 | 167535 |
| alterations in adenyl cyclase activity and glucose utilization of bordetella pertussis-sensitized mouse spleen. | | 1975 | 167788 |
| evaluation of antitumor activity of bordetella pertussis in two murine tumor models. | the antitumor activity of three preparations of killed bordetella pertussis (bp) (eli lilly crude and fluid pertussis vaccines and parke-davis pertussis vaccine) was studied in the b16 melanoma and cad2 mammary adenocarcinoma models. in these tumor systems; bp had weak and variable tumor inhibitory activity and did not augment tumor rejection immunity. the intratumor injection of bp did not affect the growth of the b16 tumor but significantly inhibited the growth of the cad2 tumor. however, the ... | 1975 | 169359 |
| leukocytosis-promoting factor of bordetella pertussis. | | 1975 | 169413 |
| proceedings: hemagglutinating substance of bordetella pertussis. | | 1975 | 169414 |
| influence of bordetella pertussis and bacterial endotoxins on the immunological reactivity of germfree mice. | as compared to specifically pathogen-free nmri mice, in principle, the immunological reactivity of germfree mice of the same strain and age was not found to be reduced. this is documented by the cellular kinetics of the primary immune responses, evoked by the intraperitoneal (i.p.) injection of either a "saturated" dose of 4 times 10(8) sheep erythrocytes (se) or the simultaneous injection of 4 times 10(8) se and 3 times 10(9) killed bordetella pertussis organisms (po). thereby, adjuvancy of po ... | 1975 | 169652 |
| studies on the adjuvant effect of bordetella pertussis vaccine to sheep erythrocytes in the mouse. i. in vitro enhancement of antibody formation with normal spleen cells. | the adjuvant effect of bordetella pertussis vaccine (pv) on the antibody response to sheep erythrocytes (srbc) has been studied in vitro with the mishell-dutton immunization technique. the addition of pv to cultures of spleen cells obtained from normal non-immunized mice markedly enhanced the plaque-forming cell response to srbc. the greatest enhancement was evident at 24 hr of culture. pv was also shown to enhance the antibody response of spleen cells that had been depleted of either t lymphocy ... | 1975 | 170337 |
| [properties of recently isolated bordetella pertussis]. | | 1975 | 170434 |
| [drug sensitivity of recently isolated bordetella pertussis and bordetella parapertussis]. | | 1975 | 170435 |
| [serotyping of recently isolated bordetella pertussis]. | | 1975 | 170436 |
| adjuvant activity of the histamine-sensitizing factor of bordetella pertussis in different strains of mice. | the effect of an extract of histamine-sensitizing factor (hsf) of bordetella pertussis on the immune response of different strains of mice to ovalbumin (oa) was investigated with regard to optimal dose of antigen and adjuvant. it was observed that all strains of mice treated with hsf during immunization with oa demonstrated enhanced production of hemagglutinating antibodies, as compared to animals treated with antigen alone. this enhancement was generally not as great as that demonstrated when a ... | 1975 | 171224 |
| 3-deoxy-2-octulosonic acid 5-phosphate: a component of the endotoxin of bordetella pertussis. | upon hydrolysis with 2 n hydrochloric acid for 2 h, a 3-deoxy-octulosonic acid 5-phosphate was released from the endotoxin of bordetella pertussis. the structure of the compound was established through chemical degradation. by periodate treatment of the intact endotoxin it was shown that positions 7 and 8 of the bound octulosonic acid phosphate were free, which, if present in a cyclic form, must be a pyranoside. | 1975 | 173533 |
| enhancement of reaginic and hemagglutinating antibody production by an extract of bordetella pertussis containing histamine sensitizing factor. | the effect of an extract containing the histamine-sensitizing factor (hsf) of bordetella pertussis on the immune response of mice to ovalbumin was investigated with respect to dose of antigen and adjuvant. of particular interest was the enhancement of reaginic antibody production. in comparison to the al(oh)3 induced production of reaginic antibody where low doses of antigen and adjuvant yield high titers of reagin, the hsf extract demonstrated optimal adjuvant activity at high doses of both adj ... | 1976 | 173756 |
| characteristics of cells present in peritoneal fluids of mice injected intraperitoneally with bordetella pertussis. | peritoneal fluids obtained from mice after the intraperitoneal administration of bordetella pertussis vaccine, heated vaccine, an extract of the organisms, killed escherichia coli, or thioglycolate medium were examined in terms of total cells and percentage that adhered to glass cover slips during 2-h incubation period. all these substances were found to increase the number of leukocytes in peritoneal fluid within 1 to 2 days after the injection. this increase appeared to be due to an influx of ... | 1976 | 175017 |
| [hemagglutination caused by leukocytosis-promoting factor of bordetella pertussis]. | | 1975 | 175184 |
| [aerosol inoculation of bordetella pertussis in mice]. | | 1975 | 175185 |
| mouse or man? which are pertussis vaccines to protect? | type 1 strains of bordetella pertussis can infect mouse brain and have been recovered as type 1 organisms after death. when introduced into the naso-pharynx of the marmoset, they immediately acquired agglutinogen 2 or 3, and the resulting type 1,2 or 1,3 infection persisted for many weeks. as in the child, agglutinogens 2 and/or 3 appear to be essential for infection of the marmoset, whereas they are quite unnecessary in mouse brain. a vaccine (extract or whole cell) containing agglutinogen 1 ma ... | 1976 | 177701 |
| the importance of agglutinin production in mice in the determination of the definitive serotype of bordetella pertussis. | a schedule for the routine serotyping of strains of bordetella pertussis based on agglutinin production in mice to the k-antigens has been worked out. mice have been found as satisfactory as rabbits but far more economical for the production of the very small volumes of serum which are required. agglutinin production, used in conjunction with direct agglutination, provides definitive information about serotype. | 1976 | 177702 |
| the serotypes of bordetella pertussis isolated in great britain between 1941 and 1968 and a comparison with the serotypes observed in other countries over this period. | classification, by agglutinogens, of 634 isolates of bordetella pertussis collected from 1971 to 1968 in great britain demonstrated that a change from a predominantly 1,2,0,4 serotype (75% of those examined during 1941-4) to a predominantly 1,0,3,0 serotype (73% of those examined during 1966-8) occurred sometime after 1953. furthermore, evidence from the examination of isolates collected between 1941 and 1953 suggests that the change may have been gradual. isolates of serotype 1,2,3,4 made up 20 ... | 1976 | 177703 |
| the stability of the serotypes of bordetella pertussis with particular reference to serotype 1,2,3,4. | strains of bordetella pertussis in which all the organisms contain agglutinogens 1 and 3 or 1,2 and 4 are easy to identify as serotypes 1,0,3,0 and 1,2,0,4 respectively; and similarly, stable strains of serotype 1,0,3,4 are occasionally found. during repeated subcultures, passage in vivo, and lyophilization and preservation for many years, these serotypes do not change. mixing 1,0,3,0 and 1,2,0,4 serotypes and culturing them together in vivo and in vitro produces cultures from which organisms of ... | 1976 | 177704 |
| the effect of bordetella pertussis on rosette-forming cells present in the peritoneal fluids of mice. | | 1976 | 177778 |
| the effect of 2, 4-dinitrophenol on the growth of bordetella pertussis in chick tracheal organ culture. | among various metabolic inhibitors tested, only 2, 4-dinitrophenol inhibited the growth of bordetella pertussis in chick tracheal organ culture at concentrations nontoxic both for bacterial organisms and for ciliary motility of the tracheal fragments. although this effect of 2, 4-dinitrophenol was reversible in its early stage, longer treatment with this inhibitor resulted in an irreversible inhibition of bacterial growth due to secondary damage of the tracheal fragments. from these observations ... | 1975 | 177795 |
| [intracytoplasmic inclusions in bordetella pertussis]. | | 1976 | 178493 |
| the production of ige and igga antibodies in normal rats and rats infected with nippostrongylus brasiliensis. | the time courses of production of ige and igga homocytotropic antibodies were measured in wistar rats during a primary and secondary response to egg albumin with pertussis or freund's adjuvants. an anamnestic ige antibody response occurred in animals previously sensitized to antigen with killed bordetella pertussis as adjuvant. igga antibodies were formed in the primary response with freund's complete adjuvant only, but were found during the secondary response with all adjuvants used. the time ... | 1976 | 178591 |
| production of circulating reaginic (ige) antibodies by oral administration of ovalbumin to rats. | reaginic antibody synthesis following parenteral and/or oral administration of ovalbumin and bordetella pertussis organisms as adjuvant has been evaluated in lou/m/wsl inbred rats. these rats are able to produce high reaginic antibody serum levels after intraperitoneal injection of this antigen. primary oral administration of ovalbumin doses between 10 and 100 mg with bordetella pertussis organisms given as adjuvant by the intraperitoneal or the oral route led to characteristic reagnic responses ... | 1976 | 179939 |
| proceedings: comparison between histamine-sensitizing factor of bordetella pertussis and bacterial endotoxin in respect to histamine-sensitizing activity. | | 1975 | 180321 |
| extracytoplasmic adenylate cyclase of bordetella pertussis. | soluble adenylate cyclase [ec 4.6.1.1] accumulates in the culture medium of exponentially growing bordetella pertussis (300-900 pmol of camp formed/min per ml of 24 hr culture supernatant). in addition, there is an extracytoplasmic adenylate cyclase which enables the intact organisms to form [32p] camp (adenosine 3':5'-cyclic monophosphate) from exogenous [alpha-32p] atp (200-1200 nmol of camp formed/min per g wet weight of cells) and which comprises 20-45% of the total adenylate cyclase activit ... | 1976 | 180529 |
| host immune responses after administration of inactivated venezuelan equine encephalomyelitis virus vaccines. ii. kinetics of neutralizing antibody responses in donors and adoptively immunized recipients. | lymphoid cell responses to immunization with various formalin-inactivated venezuelan equine encephalomyelitis (vee) virus vaccines were monitored in mice by assessment of the development of both the neutralizing antibody response in sera of spleen cell donors and the adoptive neutralizing antibody response induced by spleen cell transfer in recipients. donors immunized intraperitoneally with formalin-inactivated vee vaccine (a single dose or a dose on three consecutive days) developed early seru ... | 1976 | 181497 |
| pulmonary patterns of adenosine-3', 5'-cyclic monophosphate accumulations in response to adrenergic or histamine stimulation in bordetella pertussis-sensitized mice. | | 1976 | 182172 |
| effectiveness of orally administered bordetella pertussis vaccine in mice. | oral administration of killed bordetella pertussis organisms to mice results in increased resistance to an intracerebral infection with virulent b. pertussis cells. the rate of survival is dependent on the dose of antigen. but besides specific systemic immunity, which is persistent over a long period, also transient non-specific resistance is increased. these effects are evidently induced without penetration of bacterial substances into the circulation. | 1976 | 182602 |
| preliminary results with an oral application of killed purtussis bacteria in newborn infants. | newborn babies have been vaccinated orally on five consecutive days, with 5 droplets of a vaccine containing 10(11) killed bordetella pertussis. eight out of twelve subjects showed antibody titres varying between 1:20 and 1:80. | 1976 | 182603 |
| soluble adenylate cyclase from the culture medium of bordetella pertussis: purification and characterization. | culture medium of exponentially growing bordetella pertussis (strain 114) contains significant quantities of soluble (100,000 x g for 1 h) adenylate cyclase. the enzyme was purified by chromatography on diethylaminoethyl-cellulose and sephadex g-200. the purest material yielded a single band on sodium dodecyl sulfate-disc gel electrophoresis. it is heat labile, has a temperature optimum of 30 degrees c, a ph optimum of ph 7 to 8, and a km for adenosine 5'-triphosphate of 0.4 mm, and requires mg2 ... | 1976 | 182669 |
| [azidocillin: activity in vitro, pharmacokinetics and therapeutic results in whooping cough]. | in vitro activities of acidocillin and ampicillin were compared in 20 strains of haemophilus influenzae, 50 strains of enterococci and 4 strains of bordetella pertussis by serial dilution test. there were no significant differences between both antibiotics. on staphylococcus aureus (100 strains) and streptococcus group a (25 strains) acidocillin was effective at the same degree as phenoxymethylpenicillin. after oral administration of 0.75 g acidocillin (1 h after a standard breakfast) serum peak ... | 1976 | 183785 |
| [mast-cell rosettes in the immunized rat]. | after stimulation by ovalbumin + bordetella pertussis, rat peritoneal mast cells can form rosettes with antigen-coated erythrocytes. this phenomenon is inhibited by previous incubation of mast cells with antigen; it is attributed to apparition of membrane-bound anaphylactic antibody. the variations of percentage of rosette forming mast cells during the course of immunization are reported and compared with p.c.a. titers. | 1976 | 183872 |
| fromation of 1,6-anhydro-l-glycero-d-manno-heptopyranose during acidic hydrolysis and methanolysis of a polysaccharide from bordetella pertussis. | | 1976 | 183886 |
| hypersensitivity to histamine and systemic anaphylaxis in mice with pharmacologic beta adrenergic blockade: protection by nucleotides. | the effects of exogenous nucleotides on the histamine hypersensitivity of pharmacologically beta-blocked mice were investigated. female hla-sw (icr) mice, 27-29 gm, were injected intraperitoneally with 20 to 100 mug of propranolol 45 min before intraperitoneal challenge with 1 mg histamine. these animals had a mortality which averaged approximately 80%. at various time intervals before histamine, doses of from 0.5 to 12 mumoles of nucleotides were administered intravenously. noncyclic nucleotide ... | 1976 | 184134 |
| prevalent serotypes of bordetella pertussis in non-vaccinated communities. | in many countries, the prevalent serotypes of bordetella pertussis have changed from a mixture of types 1,2,3 and 1,2 (organisms possessing antigen 2) to a predominance of type 1,3. the timing of the change in different countries is shown to be related to the introduction of mass-vaccination with material rich in antigens 1 and 2 but weak in, or devoid of, antigen 3. in several parts of the world, there have been outbreaks of type 1,3 infection in fully vaccinated children. non-vaccinated commun ... | 1976 | 185285 |
| [structure and biological activity of bordetella pertussis endotoxin (proceedings)]. | | 1976 | 185848 |
| [increase of recirculating immunocompetent lymphocytes induced by bordetella pertussis (proceedings)]. | | 1976 | 185849 |
| separation and characterization of two distinct hemagglutinins contained in purified leukocytosis-promoting factor from bordetella pertussis. | 1. the leukocytosis-promoting factor of bordetella pertussis was found to contain two hemagglutinins with different susceptibilities to papain and separable from each other by agarose gel filtration with tris - hcl buffer containing 1 m nacl. 2. one hemagglutinin, referred to as hemagglutinin ha, had a high hemagglutinating activity, but neither leukocytosis-promoting nor histamine-sensitizing activity. the other hemagglutinin, referred to as hemagglutinin lpf appeared to be identical with the l ... | 1976 | 186106 |
| immune response against hamster erythrocytes in the low-responder mouse strains. xi. strain difference in the effects of various microbial adjuvants. | enhancing and suppressing effects of microbial adjuvants were studied in female mice of the c3h/he, akr and sl strains. propionibacterium acnes, bordetella pertussis, bcg and yeast cell wall (ycw) were chosen as adjuvants. as antigens, we chose hamster erythrocytes (hrbc) which proved to be a weak antigen for mice. adjuvants were given on day --7, day 0 or day 3, and hrbc were injected on day 0. the results were as follows. 1) p. acnes facilitated igm and igg antibody production in akr mice and ... | 1976 | 186656 |
| crossed immunoelectrophoretic analysis of bordetella pertussis antigens and of corresponding antibodies in human sera. | fourty-four antigens were demonstrated in sonicated preparations of bordetella pertussis (b.p.), using crossed immunoelectrophoresis against antiserum obtained from rabbits. no qualititative differences between the four strains of the danish pertussis vaccine were found. in preparations of b.p. culture medium, purified with respect to lymphocytosis promoting factor (lpf) activity, one antigen was possibly related to lpf. in human sera, antibodies against five of the b.p. antigens were demonstrat ... | 1976 | 187009 |
| cross-reactions between bordetella pertussis and twenty-eight other bacterial species. | cross-reactions between b. pertussis and 28 other bacterial species were studied by various quantitative immunoelectrophoretic methods. a sonicated b. pertussis antigen preparation and a corresponding pooled rabbit antiserum were used as reference system. two of the b. pertussis antigens were cross-reactive with antigens from 17, respectively 19, other bacterial species mainly gram-negative species. as judged by absorption of antibodies, the degree of cross-reactivity of these b. pertussis antig ... | 1976 | 187010 |
| role of the genetics and physiology of bordetella pertussis in the production of vaccine and the study of host-parasite relationships in pertussis. | | 1976 | 187020 |
| the in vitro effects of bordetella pertussis lymphocytosis-promoting factor on murine lymphocytes. i. proliferative response. | the lymphocytosis-promoting factor of bordetella pertussis is a potent mitogen for murine lymphocytes in vitro. the stimulatory response was not the result of specific antigen stimulation. spleen and lymph node cells were responsive, whereas normal thymocytes were unresponsive. however, dna replication was induced in cortisone-resistant thymocytes by lymphocytosis-promoting factor (lpf). bone marrow cells were not stimulated by lpf. | 1977 | 187715 |
| the in vitro effects of bordetella pertussis lymphocytosis-promoting factor on murine lymphocytes: ii. nature of the responding cells. | the mitogenic response of murine lymphocytes to the lymphocytosis-promoting factor of bordetella pertussis has been shown to be due to activation of t cells. the selectivity of responsiveness to lpf with respect to the population of t cells which is stimllated, differs from that of pha as well as con a, and the surface receptors are different. a population of adherent cells, which does not appear to consist of macrophages or other phagocytic cells, is required for the t-cell response. | 1977 | 187716 |
| 7-o-(2-amino-2-deoxy-alpha-d-glucopyranosyl)-l-glycero-d-manno-heptose. a constituent of the endotoxin of bordetella pertussis. | hydrolysis of the bordetella pertussis endotoxin, extracted from both "phase i" and "phase iv" bacteria, with 4 m hcl for 1 h at 100 degrees c, released the disaccharide named in the title; it was isolated by paper electrophoresis or by ion-exchange chromatography in about 1% yield (w/w). the structure of the heptose could be rigorously established by chemical degradation; the facts that the glucosaminidic linkage was hydrolysed by an enzyme preparation containing both, alpha and beta-n-acetylgl ... | 1976 | 188640 |
| radiolabelling of bordetella pertussis envelope proteins by the 125 i-lactoperoxidase method. | bordetella pertussis strain number 18334 was grown in media which yielded cells with either a normal complement of surface antigens (x-model), or cells which were phenotypically altered (c-model). neither x- nor c-mode bacteria incorporated more than traces of radioactivity when exposed to na 125 i, lactoperoxidase and a source of h2o2 under conditions which gave substantial labelling of bsa and other soluble proteins. in contrast, envelope preparations were readily labelled. analysis by sodium ... | 1976 | 189162 |
| study on toxicity of bordetella pertussis cultures and antigens. | the toxicity of 201 bordetella pertussis strains isolated in belgrade has been studied. the strains were grown on a modification of cohen and wheeler's medium. ld50 of examined strains ranging between 1.06 and 1.95 billion bacteria in 35 (17.41%), between 2.04 and 4.83 billion bacteria in 92 (45.77%) and between 5.31 and 7.79 billion bacteria in 46 (22.88%) were found. low toxic cultures of b. pertussis strains with ld50 ranging between 9.0 and 14.1 billion bacteria in 28 (13.43%) were obtained. ... | 1977 | 190070 |
| effects of in vivo administered b. pertussis and other adjuvants on the mitotic responses of lymphocytes in vitro. | intraperitoneal treatment of mice with adjuvants affects the in vitro response of their lymphocytes toward class-specific mitogen. spleen cells from animals injected with corynebacterium parvum organisms showed in some cases an increase in their response to all mitogens, while in other experiments, a moderate decrease in the reaction to t-specific mitogens (concanavalin a and phytohemagglutinin) was found. injection of lipopolysaccharide (lps) and in particular bordetella pertussis bacteria, bro ... | 1976 | 190170 |
| canine kidney allograft prolongation by pretreatment with bordetella pertussis. | | 1976 | 190722 |
| [isolation and purification of a preparation possessing leukocytosis-stimulating properties from pertussis bacteria]. | a homogeneous protein lsf-2 preparation was extracted from the cultural fluid of bordetella pertussis strains of the 1.0.3 serological type by means of precipitation with ammonium sulphate and electrofocussing; this preparation proved to produce a marked leukocytosis-stimulating and a weak toxic action of delayed type in experiments on animals. intraperitoneal administration of 5 mug of the lsf-2 preparation caused a rise of leukocytosis in mice to 100,000 cells per 1 mm3, a delay in the gain in ... | 1977 | 190830 |
| in vitro stimulation of human lymphocytes by bordetella pertussis. | bordetella pertussis (b.p.) induces blast transformation of human lymphocytes; whole killed b.p. are more efficient than extracts obtained by sonication. similar responses were obtained with each of the four strains used in the danish pertussis vaccine. b.p. with low amounts of protective antigen and histamine-sensitizing factor also induced lymphocyte transformation, but were less toxic to the lymphocytes at high concentrations. the supernatants of b.p. cultures were purified with respect to ly ... | 1977 | 190855 |
| effects of bordetella pertussis on the sensitivity of inbred mice to vasoactive amines. | pretreatment with bordetella pertussis was determined to increase significantly the hypovolemia induced by intravenous injections of histamine either alone or in mixture with serotonin in a total of 26 different strains of mice. two factors affecting the mortality rates observed by challenge after b. pertussis treatment were: the sensitivity of the strains to vasoactive amines before b. pertussis treatment, and their resistance to acute hypovolemic shock. appropriate crosses and backcrosses betw ... | 1977 | 193799 |
| histopathological changes in mice treated with extracts of the histamine-sensitizing factor of bordetella pertussis. | the histamine-sensitizing factor (hsf) of bordetella pertussis was isolated in a highly purified form. in addition to inducing profound sensitization to histamine, it also caused a significant lymphocytosis and produced an enhancement of reaginic antibody production in animals upon immunization with antigen. biologically active doses of hsf produced significant pathological changes in mice including congestion and edema of the lung and a marked depletion of cells in the thymus, white pulp of the ... | 1977 | 193800 |
| [toxic factors of bordetella pertussis, with special reference to leukocytosis-promoting factor, histamine-sensitizing factor and hemagglutinins (author's transl)]. | | 1976 | 194282 |
| preferential enhancement of ige antibody formation by bordetella pertussis. | preferential enhancement of ige antibody response was observed in balf/c mice by the administration of bordetella pertussis with antigen (dnp-salmonella). correlation between b cell mitogenic activity and adjuvant action among b. pertussis, salmonella, lipopolysaccharide of escherichia coli and ficoll was examined but was not found. thymus-derived cells seemed necessary to develop adjuvant action of b. pertussis since antibody response in athymic nude mice was not influenced by b. pertussis. hel ... | 1977 | 194843 |
| 2-o-(beta-d-glucuronyl)-7-o-(2-amino-2-deoxy-alpha-d-glucopyranosyl)-l-glycero-d-manno-heptose: a constituent of the bordetella pertussis endotoxin. | the presence of bound d-glucuronic acid in the endotoxin of bordetella pertussis was demonstrated. the branched chain trisaccharide named in the title was isolated after hydrolysis of the endotoxin with 3 m hcl for 2 h at 100 degrees c. its structure was established by chemical and enzymic degradation. | 1977 | 196849 |
| identification of 2-methyl-3-hydroxydecanoic and 2-methyl-3-hydroxytetradecanoic acids in the 'lipid x' fraction of the bordetella pertussis endotoxin. | the 'lipid x' fraction, released from the bordetella pertussis endotoxin upon treatment with trifluoroacetic acid of ph 3 at 50 degrees c, was shown to contain, in addition to 3-hydroxydecanoic, 3-hydroxydodecanoic, 3-hydroxytetradecanoic, and tetradec-2-enoic acids, 2-methyl-3-hydroxydecanoic-, and 2-methyl-3-hydroxytetradecanoic acids. the structure of these was established by gas-liquid chromatography/mass spectrometry. | 1977 | 196857 |
| pertussis outbreak in austin and travis county, texas, 1975. | an outbreak of bacteriologically proven pertussis occurred in austin and travis county, texas, over a 7-month period in 1975. eighty persons were cultured for pertussis in our laboratory. a total of 62% of specimens from 34 individuals with suspected pertussis was positive for bordetella pertussis. diagnosis of acute cases by both culture and fluorescent antibody was attempted, and the correlation of the methods is given. analyses of cases by age, sex, immunization status, and antibiotic treatme ... | 1977 | 197117 |