[sensitivity of bordetella bronchiseptica, pasteurella multocida and mycoplasma hyorhinis strains isolated from pigs towards antibiotics and chemotherapeutic agents]. 1978100928
bacterial and mycoplasma flora of a laboratory colony of the common marmoset (callithrix jacchus).a survey was carried out to characterise the bacterial and mycoplasma flora of a closed colony of marmosets, callithrix jacchus. bordetella bronchiseptica and staphylococcus aureus were the only potential bacterial pathogens isolated from the colony; candida albicans was also identified. s aureus and c albicans were the only organisms which were associated with macroscopic lesions or overt disease. post mortem examination of the animals proved only reliable method of establishing the presence of ...1978102075
a comparison of bacterial flora isolated by transtracheal aspiration and pharyngeal swabs in macaca fascicularis.the pulmonary flora of 30 monkeys (macaca fascicularis) was sampled by the transtracheal aspiration technique and the pharyngeal swab method, and the results were compared. the transtracheal aspiration technique yielded lower numbers of bacteria in both aerobic and anaerobic cultures. the bacteria isolated by transtracheal aspiration were predominately pure culture, thereby lowering the possibility of contamination from commensal flora. bordetella bronchiseptica was isolated from 23.3% of the mo ...1979110978
[devitalization of bordetella bronchiseptica in vitro using vapors of persterile and triethyl glycol].using the method of bacterial carriers, the devitalizing effect was studied of persteril and triethylene glycol vapours, acting in vitro, on a 24-hour culture of a mixture of 39 strains of bordetella bronchiseptica, isolated from the nasal cavity of pigs which came from five stocks suffering from the clinical form atrophic rhinitis. after exposure of the carriers of bordetella bronchiseptica to the action of spontaneously developing vapours o the mentioned chemicals, the devitalizing effect was ...1979111400
identification of nonfermentative gram-negative bacteria in the clinical laboratory.a simplified, concise scheme was developed for the identification of nonfermentative, gram-negative bacteria which have most frequently been reported in the literature as definite or possible agents of human disease. these organisms included apyocyanogenic pseudomonas aeruginosa, p. fluorescens, p. putida, p. stutzeri, p. maltophilia, p. putrefaciens, p. cepacia, p. alcaligenes, flavobacterium species, bordetella bronchiseptica, acinetobacter anitratum (herellea vaginicola), a. iwoffi (mima poly ...1975163060
relationship of porcine cytomegalovirus and b bronchiseptica to atrophic rhinitis in gnotobiotic piglets.both porcine cytomegalovirus (pcmv) and bordetella bronchiseptica produced rhinitis and pneumonia when inoculated intranasally into young gnotobiotic pigs. with pcmv the nasal lesions were confirmed to the lamina propria, while bordetella produced atrophy of the turbinate bones and hyperplasia and degeneration of the nasal epithelium. some exacerbation of the lesions was observed in the nasal mucosa of pigs given both agents, but the degree of bone atrophy was not increased.1976176765
the canine contagious respiratory disease complex (kennel cough).several infectious agents are involved in the kennel cough complex in dogs. they include canine parainfluenza virus (sv5), canine adenovirus 2, bordetella bronchiseptica, and possibly several mycoplasma species. the importance of each of these agents in the disease syndrome is discussed as well as possible prevention or treatment.1978204455
a study of dogs with kennel cough.a detailed study of a population of dogs with kennel cough was undertaken. twenty-seven (77 per cent) of a total of 35 dogs had pathological evidence of respiratory disease in the form of tracheobronchitis with, in some animals, exudative pneumonia. a variety of viral and bacterial agents were isolated from the respiratory tract of diseased dogs but bordetella bronchiseptica and canine parainfluenza virus sv-5 appeared to be the most significant organisms recovered.1978207006
phosphatase activity of aerobic and facultative anaerobic bacteria.1115 strains of aerobic and facultatively anaerobic bacteria were tested for phosphatase activity by a conventional plate method and a microtest. the microtest was devised to allow results to be read after 4 h cultivation. phosphatase activity was found in wide range of species and strains. besides staphylococci, where the test for phosphatase is successfully used, it may be applied as one of the valuable tests for the differentiation of the following species: bacillus cereus, b. licheniformis, ...1978216188
[seromonitoring of laboratory mouse and rat colonies for common murine pathogens (author's transl)].during a period from 1973 to 1978, 392 and 225 lots including 12,232 mouse and 8,044 rat individual sera, respectively, were examined for antibodies to murine hepatitis virus, sendai virus, bordetella bronchiseptica, mycoplasma pulmonis, tyzzer agents, salmonella typhimurium and corynebacterium kutscheri. of mouse lots 94.5% and 39.3% from breeder and user colonies, respectively, were negative for all antibodies examined as well as 31.6% and 17.2% of rat breeder and user colonies, respectively. ...1979225183
the recovery of bordetella bronchiseptica strains by means of smears from the nasal cavities of pigs. 1979398246
bordetella bronchiseptica infection in the lesser bushbaby (galago senegalensis). 1977404465
effect of carbadox on growth, feed utilization, and development of nasal turbinate lesions in swine infected with bordetella bronchiseptica.carbadox in combination with sulfamethazine did not interfere with the activity of sulfamethazine in clearing experimentally induced bordetella bronchiseptica nasal infection. evaluations in three field cases of infectious atrophic rhinitis indicated that carbadox in the feed was as effective as chlortetracycline + sulfamethazine + penicillin for improving growth rate and feed efficiency, as compared with nonmedicated controls, and in reducing the prevalence of lesions of infectious atrophic rhi ...1979422462
production of lesions similar to naturally occurring swine atrophic rhinitis by cell-free sonicated extract of bordetella bronchiseptica. 1979430927
efficacy of bordetella bronchiseptica bacterin in controlling enzootic atrophic rhinitis in swine.the efficacy of a bordetella bronchiseptica bacterin was evaluated in 2 commercial swine herds affected with mild and severe enzootic atrophic rhinitis (ar). in the 1st herd study, (mild ar), the degree of clinical ar, nasal turbinate evaluation, blood serum titer to b bronchiseptica antigen, and adjusted days from birth to 100 kg were determined for individual pigs. bacterin inoculation reduced the incidence and severity of gross turbinate atrophy 57% and reduced clinical ar over 93%. inoculate ...1979453686
adherence of bordetella bronchiseptica to swine nasal epithelial cells and its possible role in virulence.bordetella bronchiseptica phase i organisms adhered well to swine nasal epithelial cells cultured in vitro, while phase iii variants exhibited feeble adherence to the same cells. similarly, firm attachment of phase i organisms and poor adhesion of phase iii organisms to nasal epithelium were demonstrated in experimentally infected piglets. electron microscopic observations of nasal mucosa infected with phase i organisms showed preferential adherence to the cilia of nasal epithelial cells; the as ...1979504806
control of atrophic rhinitis with a bordetella bronchiseptica bacterin. 1977587561
prevalence of bordetella bronchiseptica infection in swine of southeastern alabama. 1977596705
vaccination against canine bordetellosis: protection from contact challenge.eight collie-cross pups, eight weeks old, were inoculated intramuscularly with an aluminum hydroxide adjuvanted preparation of killed bordetella bronchiseptica; the inoculation was repeated after two weeks. two weeks after the second inoculation, the vaccinated dogs and a control group of four unvaccinated animals were placed in contact with a group of five pups of similar age which had been experimentally infected with a pathogenic strain of b bronchiseptica by an aerosol method. all four unvac ...1978685092
an agglutination test for the detection of bordetella bronchiseptica infection in agglutination test with the use of formalin-killed antigen of the cell carrying the capsule was developed and used for the detection of antibody in swine naturally infected with bordetella bronchiseptica. under optimum antigen concentration and reaction temperature 210 or 60% of 342 serum samples tested from 42 conventional swine herds were positive for bordetella infection. in contrast, only 34 or 10% of 342 nasal swabs from the same animals were positive for bordetella by culture technique. ...1978688071
vaccination against canine bordetellosis using an aluminum hydroxide adjuvant vaccine.six collie dogs, eight weeks old, were inoculated intramuscularly with an aluminium hydroxide adjuvanted preparation of killed bordetella bronchiseptica; the inoculation was repeated after two weeks. two weeks after the second inoculation, the vaccinated dogs and a comparable group of six unvaccinated animals were challenged by exposure to an aerosol of pathogenic b bronchiseptica. all six unvaccinated control dogs developed respiratory disease characterised by persistent coughing. in contrast, ...1978705049
[atrophic rhinitis in swine].four 60-day-old pigs were experimentally infected with bordetella bronchiseptica at a state in which the animals were clinicylly normal. a single pig only, killed on the 180th day, presented a slight deflection of septum nasi. morphologically, there were severe dystrophic and necrotic changes in the mucosa of the conchae, the nasal cartilages as well as in the bones. the pathogen was reisolated from two of the pigs. clinical and morphological investigations were carried out also with spontaneous ...1978706148
vaccination against bordetella bronchiseptica infection in dogs using a heat-killed bacterial vaccine.a group of eight-week-old dogs was inoculated with a heat-killed suspension of bordetella bronchiseptica by the intramuscular route on two occasions at an interval of two weeks. all vaccinated animals developed high circulating agglutinin titres by two weeks after the second inoculation at which time the vaccinated dogs and a comparable group of unvaccinated animals were challenged by exposure to an aerosol of live b bronchiseptica. both vaccinated and unvaccinated dogs subsequently developed cl ...1978705048
acute respiratory disease in turkey poults caused by bordetella bronchiseptica-like bacteria. 1978716179
bordetella rhinitis in pigs: serum and nasal antibody response to bordetella bacterins.the nasal and serum antibody response of two groups of pigs, vaccinated with adjuvant containing formalinized or sonicated bordetella bronchiseptica bacterins was compared with the response of a nonvaccinated group. the tube agglutination test was used to determine agglutinin titers. following vaccination, all pigs were challenged intranasally with the vaccine strain of bordetella, after which the nasal bordetella flora of vaccinated and nonvaccinated pigs was investigated. sera and nasal secret ...1978647455
[nitrofurantoin-test for the differentiation of bordetella bronchiseptica (author's transl)].bordetella bronchiseptica is primarily resistant against nitrofurantoin (mic greater than 200 mug/ml), and this feature can be used for the differentiation of the organism from other gram-negative coccobacteria. nitrofurantoin paper disks (300 mug) failed to affect the growth of 150 strains of b. bronchiseptica isolated from different animal hosts, but they produced marked inhibition zones in the cultures of the followingspecies: pasteurella multocida, pasteurella pneumotropica, pasteurella haem ...1975809944
control of a tracheobronchitis epizootic associated with bordetella bronchiseptica in a closed dog colony. 1977584586
experimental infection of gnotobiotic piglets with mycoplasma hyorhinis and bordetella bronchiseptica. 1977842196
aerosol nolvasan treatment of bordetella bronchiseptica in dogs. 1977584098
canine bordetellosis: chemotherapy using a sulphadiazine-trimethoprim combination.respiratory disease was induced in young dogs by exposure to an aerosol of bordetella bronchiseptica. the affected dogs were then treated with a sulphadiazine-trimethoprim combination by daily subcutaneous injection for five days. there was marked improvement in the clinical, bacteriological and pathological features of the respiratory disease during and immediately after the treatment period but treated dogs relapsed a few days after chemotherapy was stopped. the use of a sulphadiazine-trimetho ...1979555120
pneumonia associated with bordetella bronchiseptica in captive koalas.bordetella bronchiseptica was isolated from 12 cases of purulent bronchopneumonia from a captive koala colony near brisbane, queensland. an initial epizootic in march 1967 causing 13% mortality was followed by annual outbreaks causing 2% mortality usually in newly weaned and aged koalas in late winter to early spring. high population density and a low plane of nutrition in winter were thought to predispose to the occurrence of the disease.1979543834
intranasal immunization of dogs against bordetella bronchiseptica-induced tracheobronchitis (kennel cough) with modified live-bordetella bronchiseptica vaccine. 1979525927
parenteral vaccination of young swine against bordetella bronchiseptica.the efficacy of bordetella bronchiseptica bacterins for the prevention of nasal turbinate atrophy caused by induced b bronchiseptica infection in young swine was evaluated. vaccination of pigs at an early age (eg, at 1 and 4 weeks of age) with potent b bronchiseptica strain d-1 bacterins resulted in high concentrations of circulating antibody up to 12 weeks of age or longer and in a significant (p less than 0.01) reduction in the frequency of turbinate atrophy.1979525851
effect of vaccination of sows with bordetella bronchiseptica on the incidence of atrophic rhinitis in swine.vaccination of pregnant sows with bordetella bronchiseptica was performed in an spf herd with clinical atrophic rhinitis. agglutinins against the organism were induced in serum and colostrum by the vaccination. the antibody titres were generally higher in colostrum than in corresponding serum samples. the frequency at slaughter of turbinate atrophy (+, ++, +++) was lower among pigs from vaccinated sows (11%) than in pigs borne by unvaccinated sows (20%). in the control group a lower daily weight ...1977905119
the effect of a respiratory tract infection on histamine-induced changes in lung mechanics and irritant receptor discharge in dogs.the effects of intravenously administered histamine on total lung resistance (rl), dynamic lung compliance (cdyn), and the discharge of lung irritant receptors have been measured in normal dogs and in dogs whose upper respiratory tract was naturally infected with the organism bordetella bronchiseptica. the resting values for rl and irritant receptor discharge were similar for the infected and control dogs, but cdyn was significantly lower in the infected group of dogs. intravenous administration ...1979507511
antibacterial activity of heneicomycin.heneicomycin is structurally similar to efrotomycin, mocimycin (kirromycin) and x-5108 (goldinomycin). comparisons were limited because of the small supplies available. all antibiotics show the same in vitro antibacterial spectrum although some test cultures were less sensitive to efrotomycin. heneicomycin compared favorably with efrotomycin when given subcutaneously or per os against infections with moraxella bovis and streptococcus pyogenes. the raid elimination of heneicomycin observed follow ...1979468738
[isolation of bordetella bronchiseptica strains from wild animals captured in ankara, konya, urfa and viransehir'de]. 1976933893
semiquantitative catalase test as an aid in identification of oxidative and nonsaccharolytic gram-negative bacteria.a simple and rapid semiquantitative slide catalase test useful for the identification of oxidative and nonsaccharolytic gram-negative bacteria, i.e., "nonfermenters," is described. using the interpretative criterion of time of appearance of oxygen bubbles in 3% hydrogen peroxide, three categories of nonfermenters were established. the rapid catalase producers included achromobacter xylosoxidans and achromobacter species; acinetobacter anitratus and acinetobacter lwoffii; bordetella bronchiseptic ...1979393719
evaluation of the api 20e system for identification of nonfermentative gram-negative bacteria.the api 20e system for enterobacteriaceae, recently broadened to include identification of nonfermentative gram-negative bacteria, was evaluated and compared with the conventional method for complete identification of 221 nonfermenters, which were well distributed into 48 species or biotypes and included organisms not listed in the api 20e data base. the results of 16 tests common to both systems were in close agreement. the api 20e system correctly identified 71 (43%) of the 165 organisms inclu ...1978353067
evaluation of the oxi/ferm tube system for identification of nonfermentative gram-negative bacilli.the oxi/ferm tube system designed for identification of oxidative-fermentative gram-negative bacteria was evaluated for identification of nonfermentative gram-negative bacilli by comparing it with the conventional system. the nine biochemical reactions used as the initial tests in the oxi/ferm tube system showed a close agreement with the same conventional reactions. however, the system was only 41% accurate to species or biotype for complete identification of 239 isolates which were well distri ...1978353066
antibacterial activity of efrotomycin.efrotomycin is a narrow spectrum antibiotic. among the genera tested for susceptibility in vitro it is most active against isolates of moraxella, pasteurella, yersinia, haemophilus, streptococcus and corynebacterium. the drug is as active by oral administration as by the subcutaneous route. blood levels rise rapidly to high concentrations, after oral dosing, and are prolonged. two peaks occur which may indicate biliary excretion and reabsorption. urinary excretion is minimal. the high blood conc ...1976994327
[differentiation of brucella by acrylamide-gel electrophoresis (author's transl)].the strains of brucella (tab. 1) were grown on tryptose-blood-agar at 37 degrees c, and the strains of bordetella, pasteurella and actinobacillus on blood-agar. after 24 or 48 h they were harvested and extracted in phenol-acetic acid-water solution (4:2:1) (1 ml/50 mg bacterial wet weight) at 4 degrees c over 48 h. after centrifugation (8000 x g, 1 h) 2 volumes of the supernatant were mixed with 1 volume of a 40% sucrose solution in 35% acetic acid. different volumes (0.20, 0.15 or 0.10 ml) of t ...1977331758
effect of immunization with a bordetella bronchiseptica bacterin on weight gain in weaning pigs. 1978252361
biochemical properties of a penicillin beta-lactamase mediated by r factor from bordetella bronchiseptica.the beta-lactamase specified by an r(te16) plasmid in bordetella bronchiseptica was purified 200-fold by carboxymethyl-sephadex column chromatography and electrofocusing. the enzyme has a molecular weight of 46,000 +/- 3,000 and an isoelectric point of 8.3 and was highly active against phenethicillin, oxacillin, and propicillin. the enzyme activity was inhibited by sodium chloride but not by ferrous ion. the maximal enzyme activity to benzylpenicillin was observed at ph 7.0 to 7.5 and at 40 c. i ...1975241286
letter: posttraumatic purulent meningitis due to bordetella bronchiseptica. 19751127513
[rearing of germfree guinea pigs and establishment of an spf guinea pig colony].new born guinea pigs of hartley strain derived by hysterectomy were fed commercial pellets, cow's milk, egg yolk and vitamin mixture since 0 days of age, when they were kept at 31 +/- 1 degrees c. out of 33 animals, 30 were reared for 40 days under aseptic state and they were transfered to a barrierred facility to establish an spf colony free from bordetella bronchiseptica, streptococcus zooepidemicus (animal c), salmonella spp., tyzzer's organisms, mycoplasma spp., reo 3 virus, sendai virus, ei ...1979155009
[rearing of germfree rabbits and establishment of an spf rabbit colony].baby rabbits hysterectomy-derived from conventional japanese white rabbits were reared under aseptic condition by feeding with 4 types of artificial diets. rabbit milk for the artificial diet was obtained from conventional dams at 7--25 days after delivery. the artificial diets was given by stomach tube twice a day. the total volumes of diet given (ml per day) were y = 2.3x + a (1 to approximately 14 days of age), y = 32.2 + a (l5 to approximately 25 days of age), or y = (32.2 + a) - 37.5 (x - 2 ...1979155008
[sensitivity of microorganisms isolated from swine to aqueous and proteinic solutions of gentamycin].employing a microbiologic method (in vitro) the activity was investigated of gentamicin dso pharmachim, dissolved in water, in normal swine gamma-globulin, and in normal swine polyglobulin with regard to swine strains of escherichia coli, salmonella choleraesis bordetella bronchiseptica, and pseudomonas aeruginosa as well as mycoplasms. a strong sensitivity was found to exsist in all tested strains (except for the mycoplasms) to gentamycin both in a water and in a protein solution. the in vivo t ...197880055
a selective medium facilitating the isolation and recognition of bordetella bronchiseptica in pigs.a medium was developed that in most instances allowed the isolation from pigs of bordetella (alcaligenes) bronchiseptica in pure, or virtually pure, culture from such sites as the nasal cavity, which contains many other bacteria. if not suppressed, some of the latter can inhibit, sometimes completely, the growth of the bordetellae on artificial media. besides being markedly selective, the new medium is simple to prepare, reasonably cheap and practically noninhibitory to b bronchiseptica. this or ...197942958
otitis media of guinea pigs.otitis media occurred in 177 of 1373 guinea pigs necropsied during a six-year period. streptococcus pneumoniae (20%), streptococcus zooepidemicus (15%), bordetella bronchiseptica (12%), and pseudomonas aeruginosa (11%) were the most common bacteria isolated from affected tympanic bullae. radiology and otoscopy were tested as means of antemortem screening for affected guinea pigs. radiology gave 96% accuracy in diagnosing otitis media and proved to be a more satisfactory technique than otoscopy.197614279
some factors influencing the haemolysis of bordetella bronchiseptica.strain-dependent variations in the ability of bordetella bronchiseptica to produce haemolysis on solid media exist. haemolysis is strongest at an acid reaction and will not take place if the reaction is too alkaline. peptone and glutamine inhibit haemolysis and favour growth. the vigorous growth rapidly produced an alkaline reaction which inhibits the haemolysis.19765844
comparison of the metabolism of alveolar macrophages from humans, rats, and rabbits: phorbol myristate acetate.metabolic activities of unstimulated or stimulated ams from humans, rats, and rabbits were examined and compared in vitro. rates of oxygen consumption, chemiluminescence, and glucose (1- or 6-14c) oxidation by unstimulated ams from these three species increased following stimulation of the ams by bacteria or pma. although the absolute metabolic responses of ams from humans, rats, or wild rabbits were different, the metabolic activities from each species were nearly identical when compared on the ...1978712210
isolation and molecular characterization of a novel broad-host-range plasmid from bordetella bronchiseptica with sequence similarities to plasmids from gram-positive organisms.a 2.6 kb plasmid, named pbbr1, was isolated from bordetella bronchiseptica s87. after insertion of an antibiotic resistance marker, this plasmid could be transferred into escherichia coli, bordetella pertussis, b. bronchiseptica, vibrio cholerae, rhizobium meliloti, and pseudomonas putida by transformation or conjugation. conjugation was possible only when the incp group transfer functions were provided in trans. as shown by incompatibility testing, pbbr1 does not belong to the broad-host-range ...19921321324
cloning and heterologous expression of a novel arylmalonate decarboxylase gene from alcaligenes bronchisepticus ku 1201.we have cloned and sequenced a dna fragment that encodes the arylmalonate decarboxylase (am-dase) gene from alcaligenes bronchisepticus ku 1201. the amdase gene consists of an open reading frame of 720 nucleotides, which specifies a 240-amino-acid protein of relative molecular mass (m(r)) 24734. the m(r) deduced from the amdase gene is in good agreement with that of the amdase isolated from a. bronchisepticus. no tata or ttga sequence was observed within the cloned dna fragment, but the fragment ...19921369144
prophylaxis of bordetella bronchiseptica infection in guinea pigs by intranasal vaccination with live strain ts-s34.the temperature-sensitive mutant strain, ts-s34, of bordetella bronchiseptica, which cannot grow at or above the temperature 34 degrees c, has been studied in guinea pigs. the strain grew to moderate numbers in the nasal turbinates but did not grow in the lungs. guinea pigs given strain ts-s34 developed moderate levels of serum antibody. this strain also induced very high resistance to subsequent intranasal challenge with 4,000x the 50% lethal dose of virulent strain s1 of b. bronchiseptica. in ...1978730355
[contamination and age as factors in the pathogenesis of atrophic rhinitis (author's transl)].the relationship between contamination with bordetella bronchiseptica or pasteurella multocida and the age of the piglets on the one hand and the pathogenesis of atrophic rhinitis on the other, is discussed. minimal disease-free piglets born and reared on farms on which pigs affected with clinical atrophic rhinitis were present, developed clinical atrophic rhinitis at the age of six weeks. piglets born in the central veterinary institute (cvi) of sows from herds including pigs affected with atro ...1978684734
[measures to be adopted in the prevention and treatment of atrophic rhinitis in piglets under field conditions (author's transl)].the following measures designed for the prevention of atrophic rhinitis (ar) were compared. (1) adopting the all in - all out system in separate farrowing quarters. the variation in age between piglets in a single farrowing house was confined to one to two weeks. (2) treating the piglets with a combined preparation of penicillin, chloramphenicol, oxytetracycline hydrochloride or sulphonamides on the third, sixth and twelfth days of life. (3) administering immune sera to piglets. (4) vaccinating ...1977847733
vaccination with bordetella bronchiseptica vaccine on a farm with atrophic rhinitis: an evaluation of a field experiment.on a farm where atrophic rhinitis (ar) occurred the animals were vaccinated with a bordetella bronchiseptica vaccine. the immune state of the sows was checked and also the effect of this vaccination on the antibody titre of the piglets. groups of pregnant sows were vaccinated once or twice before the partus, another group of sows only had their piglets vaccinated and a third group were used as control. the serum titres of the sows were low, not higher than 1:100 before vaccination. a double vacc ...1977857335
bordetella bronchiseptica pneumonia and bacteremia following bone marrow transplantation.bordetella bronchiseptica is a frequent cause of respiratory infections in animals but rarely causes disease in humans. we describe a patient with b. bronchiseptica pneumonia and bacteremia that developed following bone marrow transplantation. b. bronchiseptica infection persisted despite antimicrobial therapy and may have progressed because of the combined effects of the patient's underlying immunosuppression and the antimicrobial antagonism between doxycycline and ciprofloxacin.19921401019
[a case of sinus-bronchi syndrome repeatedly detected bordetella bronchiseptica from sputum]. 19921402255
bacteriological variation among bordetella bronchiseptica isolates from dogs and other species.bacteriological properties of 50 isolates of bordetella bronchiseptica were compared. phase variation, which involved colonial morphology and its associated characters of hemagglutination, hemolysis, acriflavine agglutination; crystal violet staining, flagellation, and fimbriation, occurred among these isolates. organisms representing the three observed morphotypes did not have different growth rates, nor were any differences in their bacteriological characteristics observed after repeated subcu ...1977858784
enhanced adherence of pasteurella multocida to porcine tracheal rings preinfected with bordetella bronchiseptica.adherence of 25 isolates of pasteurella multocida to porcine tracheal rings was evaluated. results indicated that adherence was not related to the isolate's origin, capsular or somatic types, dermonecrotoxin production or hemagglutination activity. the effect of a preinfection with bordetella bronchiseptica on the colonization by p. multocida was then studied. on rings infected with p. multocida alone, bacteria initially adhered to the epithelium, but within a few hours, the level of colonizatio ...19921423064
naturally occurring respiratory disease in a kennel caused by bordetella bronchiseptica.the role of bordetella bronchiseptica as a primary pathogen in nautrally occurring respiratory disease of dogs has been in question since its original isolation in 1911. a study to determine the incidence of b. bronchiseptica in a closed breeding kennel has revealed that the frequency of such isolations is closely associated with mild respiratory disease characterized by a moist, sometimes productive, cough. infection with b. bronchiseptica usually occurred shortly after weaning and produced ill ...1977870289
essential oils and antimicrobial activity of two varieties of cedronella canariensis (l.) w. et b.the qualitative and quantitative determination of the essential oils of the aerial part of two varieties of cedronella canariensis (l.) w. et b., namely, c. canariensis var. canariensis and c. canariensis var. anisata have been performed, together with the study of the antimicrobial activity of both oils. the noteworthy inhibition exhibited against bordetella bronchiseptica and cryptococcus albidus may justify the popular use of the these plants in the treatment of certain diseases of the respir ...19921434678
[bordetella-bronchiseptica pneumonia in the swine].bordetella have been frequently detectable from lesioned lung material of swine as well as from the upper respiratory tracts of clinically intact animals. pneumonia, different in extent, was produced in primarily specific pathogen free pigs by intratracheal and intranasal application of eight bordetella isolates. moderate to medium manifestations of focal pneumonia with scattered speckles developed, with severity depending on dosage and mode of infection. histologically, they were characterised ...1977901131
mouse potency assay for bordetella bronchiseptica bacterins.a potency assay for bordetella bronchiseptica bacterins has been developed using mice. the immunogenicities of three bacterins, b, c, and d, were evaluated for ability to prevent death in mice as compared with a reference standard bacterin (rsb-a). bacterins rsb-a, b, and c were evaluated for ability to prevent death in mice as compared with a reference standard bacterin (rsb-a). bacterins rsb-a, b, and c were evaluated in swine for efficacy against nasal turbinate atrophy. swine immunized with ...1977914991
[artificial rearing of rabbits free of bordetella bronchiseptica under practice conditions. preliminary report]. 1977921727
inoculation experiments with bordetella bronchiseptica strains in spf pigs.the progeny of 9 spf gilts fed a balanced ration (table i) was used in an inoculation experiment with field strains of bordetella bronchiseptica isolated in herds suffering atrophic rhinitis. acute rhinitis was produced within a week after intranasal inoculation of b. bronchiseptica into 1 to 11-day-old piglets. coughing occurred in some of the exposed pigs, but signs of pneumonia did not develop. a few pigs were killed at intervals of 1 to 3 weeks after exposure. these pigs all showed histologi ...1976934809
[occurrence and drug resistance of bacteria pathogenic to the lungs from autopsy material of swine].eight hundred and fifty-four piglets which died or were euthanized due to pneumonia or rhinitis atrophicans, were investigated during the period of 1986-1990. of the animals, 569 showed bronchopneumonia, 218 had pleuritis, pericarditis and peritonitis, 165 had rhinitis atrophicans, 58 pleuropneumonia, and 9 animals had fibrinous pneumonia. pasteurella multocida, haemophilus parasuis, bordetella bronchiseptica, actinobacillus pleuropneumoniae and pasteurella haemolytica were isolated in 59.1%, 29 ...19921481214
evaluation of an atrophic rhinitis vaccine under controlled conditions.a vaccine containing inactivated cultures of bordetella bronchiseptica, toxigenic pasteurella multocida type d and dermonecrotic p multocida type d toxoid in an oil-in-water adjuvant was given to seven sows, with seven others acting as controls. half the piglets in each litter were exposed intranasally when four days old to b bronchiseptica and when eight days old to toxigenic p multocida type d. there was considerably less sneezing in the litters of the vaccinated sows and when the piglets were ...19921496755
the isolation of bordetella bronchiseptic from an outbreak of canine pneumonia.this report describes an outbreak of bronchitis and bronchopneumonia among young dogs in a breeding kennel following the introduction of two pups from another area. bordetella bronchiseptica was isolated in heavy culture from affected animals which responded well to antibacterial therapy. there was no evidence of distemper virus involvement. important bacteriological properties of bord. bronchiseptica are discussed particularly as they relate to its known pathogenicity in the young of several sp ...1976938357
[bacteriolgical survey on bordetella bronchiseptica in various animal species (author's transl)].bordetella bronchiseptica was examined on the respiratory tract of mice, rats, mongolian gerbils, golden hamsters, guinea pigs, rabbits, household dogs and cats and cynomolgus monkeys imported from asian countries. the organisms were detected in rats, guinea pigs, rabbits, dogs, cats and monkeys, but not in mice, mongolian gerbils and golden hamsters. in rats, the organisms were isolated from 7 of 25 breeding colonies, and isolation rates ranged from 10.0 to 61.3% by colonies. in guinea pigs, 22 ...1976944138
recovery of bordetella bronchiseptica from patients with aids. 19921520777
cloning, nucleotide sequence and heterologous expression of the protective outer-membrane protein p.68 pertactin from bordetella bronchiseptica.the prn gene encoding the 68 kda protective outer-membrane protein of bordetella bronchiseptica (p.68 pertactin) was cloned, sequenced and expressed in escherichia coli. the gene was isolated by dna:dna hybridization experiments using a radioactively-labelled fragment of the homologous prn gene from bordetella parapertussis. dna sequence analysis reveals that the gene is capable of encoding a protein with a molecular mass of 93996 da (p.94); this precursor molecule is processed to form the p.68 ...19921527510
the serology of bordetella bronchiseptica isolated from pigs compared with strains from other animal species.the serology of freshly isolated strains of bordetella bronchiseptica from pigs and some other animal species was studied by the agglutination and agglutinin absorption tests. differentiation was made between heat stable o-antigens and heat labile k-antigens. all the strains examined contained a common o-antigen (1). in addition, three heat stable antigens were detected. the heat labile antigens of freshly isolated strains were found to be very different. the major antigens 2 and 3 were not foun ...19751060364
bordetella bronchiseptica pneumonia in a patient with down syndrome: a case report and review. 19921534404
bordetella bronchiseptica isolations from the nasal cavity of pigs in relation to atrophic rhinitis.the occurrence of bordetella bronchiseptica and atrophic rhinitis was studied during a one-year period in four danish sow herds. in three of the heards, the epidemiological studies revealed a relation between the occurrence of b. bronchiseptica in 3--10-week-old pigs and the presence and severity of atrophic rhinitis at slaughter. in the fourth herd no such relation was found.19761250685
disease conditions affecting common seals (phoca vitulina) around the scottish mainland, september-november 1988.common seal populations around the scottish coast were less severly devastated by phocine distemper than those on the continent. nevertheless, over a 4-month period, 59 common seals were examined postmortem. the macroscopic and histopathological lesions are described. forty-two per cent of these seals were considered to be suffering from phocine distemper. serology on 29 of the 59 seals was undertaken and the results are discussed in relation to the histopathological findings. bordetella bronchi ...19921594936
the role of bacteria in phocine distemper.the death of many seals believed to be infected with phocine distemper virus was found to be associated with a variety of mainly opportunistic bacterial pathogens. the bacteria most frequently involved were bordetella bronchiseptica, corynebacterium species and a variety of streptococci. seals dying on different parts of the coast of britain were infected with these organisms in differing proportions.19921594938
experimental respiratory disease in dogs due to bordetella bronchiseptica.young dogs of two age groups, six weeks and 12 weeks respectively, were infected by aerosol with a strain of bordetella bronchiseptica which had been isolated from a dog with pneumonia. clinical respiratory disease characterised by coughing and in some cases purulent nasal discharge was induced in both groups of infected dogs and also in dogs kept in contact. b bronchiseptica was recovered from the nasal cavity, trachea, bronchi and lung parenchyma of infected and contact animals. at necropsy, m ...19761257623
cross protection studies on bordetella bronchiseptica in mice using an intracerebral challenge model.protective activities of heat-inactivated (60 degrees c for 30 min) merthiolate preserved bordetella bronchiseptica and b. pertussis bacterins were compared in intraperitoneally immunized mice challenged intracerebrally (i.p./i.c.) or intraperitoneally (i.p./i.p.). in the i.p./i.c. assay (kendrick test), a b. pertussis bacterin protected mice against challenge with b. pertussis 18-323, as well as against phase i cytotoxic and non-cytotoxic strains of b. bronchiseptica. a b. bronchiseptica bacter ...19921626369
evaluation of nasal culturing procedures for the control of atrophic rhinitis caused by bordetella bronchiseptica in swine.control of infectious atrophic rhinitis in swine breeding herds by culturing of 3 series of nasal swab specimens from each animal, with subsequent elimination of bordetella bronchiseptica culture-positive animals, was evaluated. thirteen of 17 (77%) b bronchiseptica-infected herds experiencing clinical atrophic rhinitis were feedic rhinitis were freed of clinical signs of the disease by the use of this nasal culturing procedure. in 15 of 23 (65%) b bronchiseptica-infected herds, pigs were cultur ...1977830627
cloning and sequencing of the structural gene for the porin protein of bordetella pertussis.bordetella pertussis produces a porin protein which is a prominent outer membrane component found in both virulent and avirulent strains. n-terminal amino acid analysis of purified b. pertussis porin was performed and this amino acid sequence was used to design an oligonucleotide that was then utilized to screen a lambda gt11 library containing randomly sheared fragments of dna from b. pertussis strain 347. one clone, lambda bppor, was identified and subcloned into puc18. a portion of the dna in ...19911658537
pathogenesis of canine bordetellosis.bordetella bronchiseptica produced tracheobronchitis when administered in aerosol to specific pathogen-free dogs. clinical signs appeared to be directly related to numbers of bacteria in the trachea. electron microscopic examination revealed that each bacterium was close to one or more tracheal cilia and that a fibrillar material was radiating from the bacterial cell wall. b. bronchiseptica required 14 weeks to be cleared from the tracheas of infected dogs; in contrast, other organisms commonly ...1977404367
nasal infection of alcaligenes bronchisepticus (bordetella bronchiseptica) and lesions in newborn rabbits.the mode of experimental infection with alcaligenes bronchisepticus (bordetella bronchiseptica) of pig and rabbit origin, and lesions in the respiratory organs were examined in three groups, a, b and c, of newborn rabbits. groups a and c were free from the organism and agglutinating antibody and inoculated with the organisms of pig and rabbit origin, respectively. group b had maternal antibody and was inoculated with the organism of pig origin. establishment and persistence of infection with the ...19751091866
experimental respiratory infection with pasteurella multocida and bordetella bronchiseptica in rabbits.eight-to-10-wk-old offspring of a colony of specific pathogen free [eda:(nzw x fg)f1br] rabbits were exposed to cultures of pasteurella multocida and bordetella bronchiseptica. two groups of 9 animals each were exposed to cultures of either species of bacteria intranasally and killed 2, 7, 14, and 21 da postinoculation. five of 9 rabbits in each group developed a mucopurulent nasal discharge 4-7 da postinoculation. the remaining 4 rabbits in each group failed to develop clinical signs. the gross ...19751171337
production of recombinant bordetella pertussis serotype 2 fimbriae in bordetella parapertussis and bordetella bronchiseptica: utility of escherichia coli gene expression signals.serotype-specific fimbriae of bordetella pertussis are considered potential components of new-generation vaccines against whooping cough. attempts to characterize fimbriae, and indeed other virulence determinants, produced by b. pertussis have been frustrated on one hand by low yields from b. pertussis itself and on the other by an inability to produce native recombinant products in escherichia coli. in order to try to circumvent this problem, we have examined the expression of b. pertussis sero ...19911708358
virulence of streptococcus suis type 2 strains in newborn germfree pigs depends on determine whether the virulence of streptococcus suis type 2 is associated with the phenotype of the strain, we infected newborn germfree pigs with 10 strains of s. suis type 2 categorized by three phenotypes. in an earlier study, the phenotypes were distinguished by the presence or absence of the muramidase-released protein (mrp) and an extracellular factor (ef) and were designated mrp+ ef+, mrp+ ef- and mrp- ef-. pigs were first inoculated with bordetella bronchiseptica to predispose them t ...19921730489
intranasal infection with bordetella bronchiseptica in gnotobiotic piglets.nineteen gnotobiotic piglets, four to six days old, from three different litters, were inoculated intranasally on three consecutive days with a pure culture of bordetella bronchiseptica. necropsy 10 to 30 days after inoculation revealed atrophic rhinitis lesions in all and pneumonia in 13 (73 per cent)infected piglets. one died of septicaemia two days after inoculation. agglutinating antibodies were detected in the sera of all piglets necropsied from 17 to 30 days after inoculation. five control ...19761265353
kennel cough: vaccination against bordetella bronchiseptica infection. 19761266000
electron microscopic evaluation of bone cells in pigs with experimentally induced bordetella rhinitis (turbinate osteoporosis).the development of osseous lesions in the nasal turbinates of pigs with experimentally induced bordetella rhinitis, occurring 2 to 6 weeks after inoculation with bordetella bronchiseptica strain b, was evaluated ultrastructurally, and comparisons were made with controls. progressive degenerative changes were seen in osteoblasts and osteocytes of diseased pigs. these changes included swollen mitochondria, distention of cisternae of endoplasmic reticulum, and lysis of some cells. bacterial organis ...19751115417
the bvgas locus negatively controls motility and synthesis of flagella in bordetella bronchiseptica.the products of the bvgas locus coordinately regulate the expression of bordetella virulence factors in response to environmental conditions. we have identified a phenotype in bordetella bronchiseptica that is negatively controlled by bvg. environmental signals which decrease (modulate) the expression of bvg-activated genes lead to flagellum production and motility in b. bronchiseptica. wild-type (bvg+) strains are motile and produce peritrichous flagella only in the presence of modulating signa ...19921370665
bordetella bronchiseptica in a closed cat colony. 19911763473
immunological characterization of the lipooligosaccharide b band of bordetella pertussis.two structurally and immunologically different components of bordetella pertussis endotoxin can be visualized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and silver staining: a major a band and a faster-migrating minor b band. certain mutant strains of b. pertussis express only the b band, while the wild-type strains produce both lipooligosaccharides (los). two monoclonal antibodies (mabs) directed against the minor los b band were generated, allowing the study of this surface m ...19921377181
experimental atrophic rhinitis in 2 and 4 month old pigs infected sequentially with bordetella bronchiseptica and toxigenic type d pasteurella multocida.experimental infections with bordetella bronchiseptica and/or toxigenic type d pasteurella multocida were studied in 2- and 4-month-old primary specific-pathogen-free pigs. none of the 2-month-old pigs inoculated with b. bronchiseptica or p. multocida alone developed turbinate atrophy. all the pigs inoculated with b. bronchiseptica (10(7) cfu/head) and p. multocida (10(9) cfu/head for 5 consecutive days) together, however, developed clinical and post-mortem signs of atrophic rhinitis (ar) simila ...19921385667
canine parainfluenza type 2 and bordetella bronchiseptica infection produces increased bronchoalveolar lavage thromboxane concentrations in beagle puppies.acute infections in beagle puppies with canine parainfluenza virus type 2 (cp12), and cp12 in combination with bordetella bronchiseptica (bb) produce bronchiolitis and increased airways responsiveness to aerosolized histamine during the acute infection. in order to determine whether these observations were associated with increased levels of eicosanoids, the stable metabolites of thromboxane a2 and prostacylin, thromboxane b2 (txb2) and 6-keto-prostaglandin f1 alpha (6-keto-pgf 1 alpha) respecti ...19911667042
comparison of the inhibitory effect of sulfamonomethoxine and other sulfonamides on capsule formation of bordetella bronchiseptica.the inhibitory effect of sulfamonomethoxine and other sulfonamides on the capsule formation of sulfonamide-resistant bordetella bronchiseptica was investigated. all the sulfonamides having meo(-och3) groups inhibited the capsule formation of b. bronchiseptica. strong inhibition was obtained with sulfamonomethoxine. inhibition was not seen with sulfonamides having no meo groups.19921420559
characterization of the common antigenic lipopolysaccharide o-chains produced by bordetella bronchiseptica and bordetella parapertussis.representative strains of bordetella bronchiseptica and b. parapertussis were found to produce smooth lipopolysaccharides (lps) having identical antigenic o-polysaccharide components composed of linear unbranched polymers of 1,4-linked 2,3-diacetamido-2,3-dideoxy-alpha-l-galacto-pyranosyluronic acid residues. these lpss differed from the lps of b. pertussis which produces only rough-type lps, devoid of o-polysaccharide. while b. bronchiseptica and b. parapertussis had chemically and immunologica ...19921427018
evaluation of a guarded bronchoscopic method for microbial sampling of the lower airways in foals.a novel method to reduce contamination of the bronchoscope during microbial sampling of the lower airways of foals was evaluated. methylene blue (mb) was used as a nasopharyngeal dye marker to assess the relative contamination from the upper airways of bronchoalveolar lavage (bal) specimens obtained by standard bronchoscopy (sb) and a "guarded" bronchoscopic method (gb). for gb, a clear sterile cellulose sheath was fitted over the bronchoscope in an effort to protect the endoscope tip and channe ...19911790487
[the tenacity of bordetella bronchiseptica in the air].the survival time of bordetella bronchiseptica (phase i and iii) has been determined at a temperature of 21 degrees c and 27 degrees c and at a relative humidity of 32%, 58% and 76% in a rotating aerosol chamber. the highest survival time (118.8 min half-life time) has been found at 21 degrees c and 76% relative humidity in b. bronchiseptica (phase i). an increasing temperature (from 21 degrees c to 27 degrees c) causes a lower survival time. the phase i strains react more sensitive to this chan ...19921455946
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