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studies on regulatory functions of malic enzymes. v. comparative studies of malic enzymes in bacteria.screening of four malic enzymes--nad-linked enzyme [ec 1.1.1.38], nad, nadp-linked enzyme [ec 1.1.1.39], nadp-linked enzyme [ec 1.1.1.40], and d-malic enzyme--was carried out with cell-free extracts of the following 16 strains of bacteria by the aid of sepharose 6b column chromatography: 9 strains of enteric bacteria, 3 strains of pseudomonas, alcaligenes faecalis, agrobacterium tumefaciens, rhodospirillum rubrum, and clostridium tetanomorphum. all the strains tested contained at least one malic ...197896110
[contamination of ultrasonic equipment for the hand disinfection by chlorhexidine-resistant alcaligenes faecalis (author's transl)]. 1978103976
a selective medium facilitating the isolation and recognition of bordetella bronchiseptica in pigs.a medium was developed that in most instances allowed the isolation from pigs of bordetella (alcaligenes) bronchiseptica in pure, or virtually pure, culture from such sites as the nasal cavity, which contains many other bacteria. if not suppressed, some of the latter can inhibit, sometimes completely, the growth of the bordetellae on artificial media. besides being markedly selective, the new medium is simple to prepare, reasonably cheap and practically noninhibitory to b bronchiseptica. this or ...197942958
alcaligenes faecalis septicaemia.a case of alcaligenes faecalis septicaemia occurring after appendicectomy is described, and previous reports of this condition are briefly reviewed. alcaligenes is frequently found in the faeces of healthy people and systemic infections appear to be very uncommon. nevertheless, if the organism is cultured from the blood it should not be dismissed as a contaminant without careful investigation.19761063929
isolation and properties of a particulate fraction for the desaturation of palmitic acid from alcaligenes faecalis.a particulate fraction obtained from alcaligenes faecalis could desaturate palmitic acid to palmitoleic acid. nadph, atp, coa, fe2+ and mg2+ were essential cofactors for the reaction. the desaturation showed an absolute requirement for o2. metal ions like mn2+, mo6+ and cu2+ did not affect the desaturation, while zn2+ was inhibitory. sulfhydryl agents such as cysteine, glutathione and beta-mercaptoethanol had no effect, but sh-blocking agents like hgcl2 and p-hydroxymercuribenzoate inhibited the ...197939619
molecular polymorphism and mechanisms of activation and deactivation of the hydrolytic function of the coupling factor of oxidative phosphorylation.the 13s coupling factor of oxidative phosphorylation from alcaligenes faecalis has a latent adenosine triphosphatase (atpase) function that can be activated by heating at 55 degrees c for 10 min at ph 8.5 in 50% glycerol. the specific activity increases from 0.1 to 20--30 mumol min-1 mg-1. adenosine 5'-triphosphate (atp) is not required for stabilization at 55 degreesc when glycerol is present. activation involves displacement of the endogenous atpase inhibitor subunit (epsilon subunit), and rea ...19769131
the purification and characterization of arsenite oxidase from alcaligenes faecalis, a molybdenum-containing hydroxylase.the purification and initial characterization of arsenite oxidase from alcaligenes faecalis are described. the enzyme consists of a monomer of 85 kda containing one molybdenum, five or six irons, and inorganic sulfide. in the presence of denaturants arsenite oxidase releases a fluorescent material with spectral properties identical to the pterin cofactor released by the hydroxylase class of molybdenum-containing enzymes. azurin and a c-type cytochrome, both isolated from a. faecalis, each serves ...19921331097
incidence of bacteremia in adults without infection.to determine the incidence of bacteremia in healthy adults, blood cultures were obtained from 240 patients who had no demonstrable foci of infection. five patients (2.1%) had positive blood cultures. staphylococcus epidermidis was isolated from four patients and alcaligenes faecalis from one. in each of these patients, the organism isolated probably represented contamination rather than bacteremia.1976972185
[a case of subacute bacterial endocarditis related to alcaligenes faecalis].alcaligenes faecalis was isolated from the blood culture of an 18 years old patient with subacute bacterial endocarditis and rheumatic valvular disease. isolated strain agglutinated with patient's serum at a ratio of 1/160.1977882025
[the construction of genomic library of alcaligenes faecalis and cloning of nifh gene sequence homologous to klebsiella pneumoniae].a genomic library of alcaligenes faecalis a-15hl which possesses rather high nitrogenase activity has been constructed. the total dna of a. faecalis a-15 hl was partially digested with sau3ai. 13-20 kb of fragments recovered from agarose gel were cloned in bacteriophage em-bl4 vector. a total number of 1.2 x 10(6) of recombinants was obtained. it is much beyond the desired capacity of a library. by using nifh gene of k. pneumoniae from plasmid pgb1 as probe, we have successfully screened the clo ...19921466914
[attempt to establish genome similarity between alcaligenes faecalis and several gram-negative bacteria using the method of molecular hybridization of dna to dna].the technique of dna-dna molecular hybridization was used to demonstrate the absence of genome similarity between alcaligenes faecalis and gram-negative bacteria belonging to the genera pseudomonas, enterobacterium, comamonas producing alkalis, and vibrions. some similarity in the polynucleotide sequences was found between the dna of the reper strain of alcaligenes faecalis 45 (5--10% of homology) and the dna of the culture belonging to the achromobacter genus; therefore, the two genera may belo ...1977870806
microbial fermentation of rice straw: nutritive composition and in vitro digestibility of the fermentation products.rice straw was fermented with cellulomonas sp. and alcaligenes faecalis. microbial cells and undigested residue, as well as chemically treated (naoh or nh4oh) and untreated straws, were analyzed for nutrient composition and in vitro digestibility. in a typical fermentation, 75% of the rice straw substrate was digested, and 18.6% of the total substrate weight that disappeared was recovered as microbial protein. the microbial cell fraction was 37% protein and 5% crude fiber; the residue was 12% pr ...1975804853
the effect of the fungus clavatia utriformis on the morphological picture and physiological behaviour of bacteria.when investigating the effect of various fungi on various bacteria, we found that the edible fungus clavatia caelata (utriformis) affected the bacteria alcaligenes faecalis in a triple way. a large zone of inhibition of these bacteria was found around the piece of this fungus. bacteria growing in the outer, i.e., bacteriostatic zone, were morphologically changed/mostly elongated, but also thickened) and also enhanced in their haemolysis. these features are similar to those of some bacteria when ...1978749416
carbon substrate utilization studies of some cultures of alcaligenes denitrificans, alcaligenes faecalis, and alcaligenes odorans isolated from clinical specimens.one hundred and sixty-two cultures of alcaligenes species (a. denitrificans, a. faecalis, and a. odorans) of clinical origin were characterized by routine diagnostic and carbon substrate utilization techniques. the microorganisms were tested for their ability to utilize a total of 188 substrates. substrate utilization was assayed by (i) growth stimulation and (ii) substrate alkalinization. the a. denitrificans and a. odorans cultures had unique substrate utilization profiles for each species. th ...1978701468
dependence on chain length of antitumor activity of (1 leads to 3)-beta-d-glucan from alcaligenes faecalis var. myxogenes, ifo 13140, and its acid-degraded products.the well-defined (1 leads to 3)-beta-d-glucan with (dpn) 540, produced by cultivation of alcaligenes faecalis var. myxogenes (ifo 13140), a mutant of a soil bacterium, had marked inhibitory activity against the s.c.-implanted sarcoma 180 at 5 to 50 mg/kg for 10 days. it also exhibited very high activity in doses of 60 and 100 mg/kg i.p. by a single injection at 7 days after the initial s.c. transplantation of sarcoma 180. the inhibition ratios observed with ehrlich carcinoma, ntf (nakahara-tokuz ...1978620409
spontaneous mutation of polysaccharide production in alcaligenes faecalis var. myxogenes 10c3.alcaligenes faecalis var. myxogenes 10c3, which produces large amounts of succinoglucan and small amounts of curdlan, was genetically unstable and mutated spontaneously to a form producing more curdland than succinoglucan when stocked on nutrient agar slants. the mutation occurred in the absence of cell division when the cells were incubated in saline and was enhanced by treatment with n-methyl-n'-nitro-n-nitrosoguanidine, ethyl methane sulfonate, or ultraviolet light. mutant strains were geneti ...1977596866
improvements in a non-proprietary radiometric medium to allow the detection of some pseudomonas species and alcaligenes faecalis.the addition of [5-14c]glutamate and [14c]formate to a non-proprietary medium containing [14c]glucose, trypticase, yeast extract, thiotone, and salts enabled the radiometric detection of the presence of nonfermenters of glucose. it did not interfere with the rapid detection of the presence of aerobic and anaerobic sporeforemers and nonsporeformers.1975809010
effect of experimental dermatophyte infection on cutaneous flora.the cutaneous aerobic bacterial flora was monitored during the course of experimental dermatiphyte (ringworm) infections on the forearms of 9 volunteers. micrococcaceae were identified by the new baird-parker classification with the aid of a replica-plating technique. there were significant differences in total populations but not in kinds of flora compared with control (opposite) forearms. the proportion of penicillin-resistant microorganisms, however increased the infection, to a degree which ...19751039027
specificity of bacteriolytic enzyme ii from a soil amoeba, hartmannella glebae.two bacteriolytic enzymes were produced when hartmanella glebae was grown in the presence of both enterobacter aerogenes and alcaligenes faecalis. the identification of enzyme i as n-acetylmuramidase was reported earlier. enzyme ii was purified by gel filtration on a bio-gel a column. a recovery of 68.76% with 72.3-fold purification was obtained. it was found that 5 and 10 mm mgcl2 significantly increased the bacteriolytic activity. it is a basic protein. the cell walls of micrococcus lysodeikti ...1979533270
purification and specificity of bacteriolytic enzyme i from hartmannella glebae.a soil amoeba (hartmannella glebae), when grown in conjunction with enterobacter aerogenes and alcaligenes faecalis, produced two enzymes. enzyme i was purified by gel filtration on sephadex g-100 and chromatography on diethylaminoethyl-cellulose. it is a basic protein. the analysis of the enzymic digest of the cell walls of micrococcus lysodeikticus after reduction and acid hydrolysis showed that the enzyme cleaved the glycosidic bond between acetylmuramic acid and acetylglucosamine of the pept ...1977402108
a special report: four-year study of a boy with combined immune deficiency maintained in strict reverse isolation from birth.a 4-year study of a boy with combined immune deficiency is presented, and the impact of this disease on various aspects of his growth and development is examined. there is no evidence of immune deficiency in either parent or in the genetic background on the maternal side. three children of a brother of the mother's father may have had immune deficiencies but two have grown to be teenagers with no problems. another died. at autopsy, however, lymph nodes appeared normal. the deceased older brother ...1977401538
o-agglutinin antibodies against alcaligenes faecalis and escherichia coli in hepatitis cases and normal subjects--a preliminary communication. 1978355133
preliminary observations on the application of the multiple inocula (replicator) method for carbon substrate utilization studies of alcaligenes faecalis.the use of the replicator technique in evaluating carbon source utilization by 55 cultures of alcaligenes faecalis was examined. six of the 20 substrates tested were utilized by the bacterial cultures. in this study, the reproducibility of the technique was 100%.1977323288
phosphatase activity of aerobic and facultative anaerobic bacteria.1115 strains of aerobic and facultatively anaerobic bacteria were tested for phosphatase activity by a conventional plate method and a microtest. the microtest was devised to allow results to be read after 4 h cultivation. phosphatase activity was found in wide range of species and strains. besides staphylococci, where the test for phosphatase is successfully used, it may be applied as one of the valuable tests for the differentiation of the following species: bacillus cereus, b. licheniformis, ...1978216188
characterization of aerobic desaturation of palmitic acid using resting cells of alcaligenes faecalis. 1979535934
nucleotide sequence, secondary structure and evolution of the 5s ribosomal rna from five bacterial species.the nucleotide sequences of the 5s ribosomal rnas of the bacteria agrobacterium tumefaciens, alcaligenes faecalis, pseudomonas cepacia, aquaspirillum serpens and acinetobacter calcoaceticus have been determined. the sequences fit in a generally accepted model for 5s rna secondary structure. however, a closer comparative examination of these and other bacterial 5s rna primary structures reveals the potential of additional base pairing and of multiple equilibria between a set of slightly different ...19852408888
studies on bacteria resembling alcaligenes faecalis. 1979429047
oxidation of arsenite to arsenate by alcaligenes faecalis.alcaligenes faecalis, resistant to the toxic effects of 0.01 m sodium arsenite, was isolated from raw sewage and shown to be capable of oxidizing arsenite to arsenate. when the organisms were grown in chemically defined medium, this conversion was due to the appearance at stationary phase of an intracellular, oxygen-sensitive, inducible enzyme and/or component of the electron transport system; when the organisms were grown in a nutrient broth-yeast extract medium, the enzyme appeared in the late ...197610837
mechanism of agrobacterium beta-glucosidase: kinetic studies.the beta-glucosidase from agrobacterium faecalis (previously alcaligenes faecalis) has been subjected to a detailed kinetic investigation with a range of substrates to probe its specificity and mechanism. it has a relatively broad specificity for the substrate sugar moiety and exhibits a classical ph dependence for its kinetic parameters with three different substrates and an identical ph dependence for its inactivation by a mechanism-based inactivator, cyclophellitol. measurement of kcat and km ...19921390780
heterotrophic nitrification and aerobic denitrification in alcaligenes faecalis strain tud.heterotrophic nitrification and aerobic and anaerobic denitrification by alcaligenes faecalis strain tud were studied in continuous cultures under various environmental conditions. both nitrification and denitrification activities increased with the dilution rate. at dissolved oxygen concentrations above 46% air saturation, hydroxylamine, nitrite and nitrate accumulated, indicating that both the nitrification and denitrification were less efficient. the overall nitrification activity was, howeve ...19921416919
extracellular poly(hydroxyalkanoate) depolymerases and their inhibitor from pseudomonas lemoignei.enzymatic degradation processes of microbial copolyesters, poly(3-hydroxybutyrate-co-3-hydroxyvalerate): p(3hb-co-3hv) and poly(3-hydroxybutyrate-co-4-hydroxybutyrate): p(3hb-co-4hb), were studied by the weight loss (erosion) of copolyester films. these studies employed three extracellular depolymerases which degrade poly(3-hydroxybutyrate): p(3hb). two enzymes were purified from the culture supernatant of pseudomonas lemoignei and one from alcaligenes faecalis t1. the rate of enzymatic degradat ...19921504046
synthesis and antitumor activities of mitomycin c (1----3)-beta-d-glucan conjugate.the conjugate of mitomycin c (mmc) with linear (1----3)-beta-d-glucan from alcaligenes faecalis var. myxogenes ifo 13140 was synthesized and its antitumor activities investigated. the conjugate (mmc-carboxymethylated linear (1----3)-beta-d-glucan (cmps)) was obtained by treatment of cmps with mmc in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide. in vitro cytotoxicity of mmc-cmps against l1210 leukemia cells was similar to that of mmc. in i.p.-i.p. system in vivo against p388 leu ...19921525956
a 13c nuclear magnetic resonance study of gel-forming (1 goes to 3)-beta-d-glucans. evidence of the presence of single-helical conformation in a resilient gel of a curdlan-type polysaccharide 13140 from alcaligenes faecalis var. myxogenes ifo 13140.a 13c nuclear magnetic resonance (nmr) study of a resilient gel of a curdlan-type polysaccharide 13140, a (1 goes to 3)-beta-d-glucan, from alcaligenes faecalis var. myxogenes ifo 13140, was performed in an effort to understand the gel structure. it was found that very broad 13c resonance peaks of line widths ca. 150 (c-1-c-5) and 50 hz (c-6) are able to be seen in the gel state. because of a fivefold increase of the peak intensities with respect to those in an aqueous suspension by gelation, th ...1977843521
homeostasis as regulated by activated macrophage. iii. protective effect of lpsw (lipopolysaccharide (lps) of wheat flour) on gastric ulcer in mice as compared with those of other lps from various sources.protective effect of lipopolysaccharide (lps) from various sources on gastric ulcer has been examined in mice using parenteral as well as oral route. ulcer is induced by indomethacin, stress or alcohol. lps was prepared from 6 species of bacteria (escherichia coli, pantoea agglomerans, serratia ficaria, enterobacter cloacae, bordetella pertussis, alcaligenes faecalis) and from wheat flour. when administered intravenously, lps of pantoea agglomerans was the most effective among other lps examined ...19921525959
evaluation of the 4-hour rapid nf plus method for identification of 345 gram-negative nonfermentative rods.the ability of the rapid nf plus system (innovative diagnostic systems, inc., atlanta, ga.) to identify 345 nonfermentative gram-negative rods was evaluated. kits were inoculated with no. 1 mcfarland suspensions, and reactions were interpreted after a 4-h incubation at 35 degrees c. overall, the method correctly identified 311 strains (90.1%) without additional tests and 21 strains (6.1%) with additional tests, and 13 strains (3.8%) were misidentified. five of 13 misidentified strains were alcal ...19921583129
site-directed mutagenesis of pseudoazurin from alcaligenes faecalis s-6; pro80ala mutant exhibits marked increase in reduction potential.pseudoazurin (a blue copper protein or cupredoxin) of a denitrifying bacterium alcaligenes faecalis s-6 is a direct electron carrier for a cu-containing nitrite reductase (nir) of the same organism. site-directed mutagenesis of the pseudoazurin was carried out using an escherichia coli expression system. replacement of tyr74 by phe to remove an internal hydrogen bond in the beta-barrel caused a slight decrease in heat stability as well as a requirement for a higher concentration of cu2+ for prod ...19921594573
na+/h+ exchange modulates rat neutrophil mediated tumor cytotoxicity.rat neutrophils stimulated with phorbol 12-myristate 13-acetate, bacillus calmette-guérin, zymosan a, and beta-1,3-d-glucan from alcaligenes faecalis showed cytotoxicity to various tumor cells. hydrogen peroxide was shown to be an effector molecule in tumor cytotoxicity by inhibition using various active oxygen scavengers. the following findings suggest that tumor cytotoxicity by rat neutrophils stimulated with the four reagents mentioned above is regulated by na+/h+ exchange: (a) an increase in ...19911645615
production of r-(-)-mandelic acid from mandelonitrile by alcaligenes faecalis atcc 8750.r-(-)-mandelic acid was produced from racemic mandelonitrile by alcaligenes faecalis atcc 8750. ammonium acetate or l-glutamic acid as the carbon source and n-butyronitrile as the inducer in the culture medium were effective for bacterial growth and the induction of r-(-)-mandelic acid-producing activity. the r-(-)-mandelic acid formed from mandelonitrile by resting cells was present in a 100% enantiomeric excess. a. faecalis atcc 8750 has an r-enantioselective nitrilase for mandelonitrile and a ...19911660699
antitumor activity of polymorphonuclear leukocytes activated by a beta-1,3-d-glucan.the antitumor activity of mouse polymorphonuclear leukocyte (pmn) treated with a beta-1,3-d-glucan from alcaligenes faecalis var. myxogenes ifo 13140 (tak-n) and its carboxymethylated derivative (cm-tak) was investigated in vitro and in vivo. icr mouse pmn showed strong cytotoxicity against sarcoma 180 cells and inhibition of the growth of the tumor cells in vitro in the presence of tak-n but not in the presence of cm-tak. since the cytotoxicity induced by tak-n was almost completely inhibited b ...19911779406
resonance raman spectra of plastocyanin and pseudoazurin: evidence for conserved cysteine ligand conformations in cupredoxins (blue copper proteins).new resonance raman (rr) spectra at 15 k are reported for poplar (populus nigra) and oleander (oleander nerium) plastocyanins and for alcaligenes faecalis pseudoazurin. the spectra are compared with those of other blue copper proteins (cupredoxins). in all cases, nine or more vibrational modes between 330 and 460 cm-1 can be assigned to a coupling of the cu-s(cys) stretch with cys ligand deformations. the fact that these vibrations occur at a relatively constant set of frequencies is testimony t ...19911932014
enhancement of polymorphonuclear leukocyte-mediated tumor cytotoxicity by serum factor(s).it has been reported that beta-1,3-d-glucan isolated from alcaligenes faecalis (tak) promoted tumor cytolysis by mouse polymorphonuclear leukocytes (pmn). we investigated the effect of serum on mouse pmn tumor cytolysis induced by tak and other pmn stimulators. addition of fetal calf serum (fcs) to the cytolysis assay enhanced tumor cytolysis by pmn in a dose-dependent manner. sera obtained from horses, mice, and rats were also effective enhancers of pmn tumor cytolysis. when fcs was added after ...19902108949
isolation and sequencing of the alcaligenes denitrificans azurin-encoding gene: comparison with the genes encoding blue copper proteins from pseudomonas aeruginosa and alcaligenes faecalis.the gene (azu) encoding azurin from alcaligenes denitrificans has been cloned and sequenced. the gene codes for a pre-protein with a 19-aa signal peptide. comparison with the sequences coding for the blue copper proteins from pseudomonas aeruginosa and alcaligenes faecalis reveals the presence of ntra and fnr boxes in front of all three genes, instead of a regular [-10, -35]-promoter. in p. aeruginosa, the azu gene is terminated by a bidirectional terminator and flanked by open reading frames on ...19902116366
an adenosine triphosphate-dependent deoxyribonuclease from alcaligenes faecalis.a deoxyribonuclease was purified approx. 800-fold from crude extracts of the bacterium alcaligenes faecalis. the enzyme requires atp and mn2+; atp could be replaced by any other ribo- or deoxyribo-nucleoside triphosphate, and mn2+ could be replaced by mg2+ in 0.1 m-tris/hcl, ph 8.0 at 37 degrees c. the enzyme could degrade linear duplex or denaturated dna, but was inactive with closed-circular duplex dna from bacteriophase pm-2. in the course of nucleolytic activity, atp was hydrolysed. we have ...1977141925
production and immobilization of d-aminoacylase of alcaligenes faecalis da1 for optical resolution of n-acyl-dl-amino acids.the production of d-aminoacylase by alcaligenes faecalis da1 was induced 5- to 50-fold by n-acetyl-d-amino acids. this strain produced about 443 units of d-aminoacylase and 52 units of l-aminoacylase per gram of cells (wet weight) when cultivated in a medium containing 1% n-acetyl-dl-leucine as the carbon source. the d-aminoacylase was partially purified by fractogel deae 650 column chromatography and then immobilized on another fractogel deae 650 column. the catalytic activity of the immobilize ...19921368114
[the exocellular o-antigen polysaccharide of alcaligenes faecalis]. 19852477670
contribution of permeability and sensitivity to inhibition of dna synthesis in determining susceptibilities of escherichia coli, pseudomonas aeruginosa, and alcaligenes faecalis to ciprofloxacin.to examine the correlation between bacterial cell susceptibility to ciprofloxacin and the magnitude of uptake and cell target sensitivity, the relative contribution of ciprofloxacin accumulation in intact cells and its ability to inhibit dna synthesis were investigated among strains of escherichia coli, pseudomonas aeruginosa, and alcaligenes faecalis. uptake studies of [14c]ciprofloxacin demonstrated diffusion kinetics for p. aeruginosa and e. coli. ciprofloxacin was more readily removed from e ...19892510591
aromatic amine dehydrogenase from alcaligenes faecalis. 19873037269
quantitative selection of denitrifying bacteria in continuous cultures and requirement for organic carbon. i. starch.a mixed population of bacteria from bottom sludge of nitrogen wastewater reservoir was incubated in continuous culture in medium containing 1000 mg nitrate nitrogen/l and starch. maximal efficiency of denitrification was 5 mg n/l/h. marked changes in participation of denitrifying bacteria (16-76%) among total number of bacteria was observed, this being dependent on the ratio of starch concentration (cs) to nitrogen concentration (cn) in the medium. the optimal cs/cn ratio ensuring highest partic ...19852579531
cloning, nucleotide sequence, and expression in escherichia coli of the gene for poly(3-hydroxybutyrate) depolymerase from alcaligenes faecalis.the extracellular poly(3-hydroxybutyrate) depolymerase gene from alcaligenes faecalis t1 was cloned into escherichia coli dh1 by using the plasmid puc8. an a. faecalis t1 genomic library was prepared in e. coli from a partial sau3ai digest and screened with antibody against the depolymerase. of the 29 antibody-positive clones, 1 (pdp14), containing about 4 kilobase pairs of a. faecalis t1 dna, caused expression of a high level of depolymerase activity in e. coli. the enzyme purified from e. coli ...19892644188
conduritol aziridine: a new mechanism-based glucosidase inactivator.a new mechanism-based glucosidase inactivator, conduritol aziridine (1,2-dideoxy-1,2-epimino-myo-inositol), has been synthesised from myo-inositol. this aziridine inactivates both the beta-glucosidase from alcaligenes faecalis and the alpha-glucosidase from yeast according to the expected pseudo-first order kinetics. inactivation constants measured are ki = 3.0mm, ki = 0.077 min-1 for the beta-glucosidase, and ki = 9.5mm, ki = 0.39 min-1 for the alpha-glucosidase. evidence for irreversible inact ...19892673241
heterotrophic nitrification by alcaligenes faecalis: no2-, no3-, n2o, and no production in exponentially growing cultures.heterotrophic nitrification by alcaligenes faecalis dsm 30030 was not restricted to media containing organic forms of nitrogen. in both peptone-meat extract and defined media with ammonium and citrate as the sole nitrogen and carbon sources, respectively, no2-, no3-, no, and n2o were produced under aerobic growth conditions. heterotrophic nitrification was not attributable to old or dying cell populations. production of no2-, no3-, no, and n2o was detectable shortly after cultures started growth ...19892782877
comparative in vitro activity of ciprofloxacin against aerobic and anaerobic bacteria from clinical isolates.1-cyclopropyl-6-fluoro-1,4-dihydro-4-oxo-7-piperazin-1- ylquinoline-3-carboxylic acid (ciprofloxacin, bay o 9867, ciprobay) is a broad spectrum antibiotic of the 4-quinolone group. it possesses a bactericidal effect attributable to the property of dna-gyrase inhibition. the antimicrobial action comprises all grampositive strains (including streptococcus faecalis) and gramnegative strains (including pseudomonas aeruginosa and serratia spp.), as well as bacteroides fragilis and other bacteroides s ...19872822054
size of diffusion pore of alcaligenes faecalis.the diffusion pore of the outer membrane of alcaligenes faecalis was shown to be substantially smaller than the escherichia coli porin pore. in experiments with intact cells, pentoses and hexoses penetrated into the nacl-expanded periplasm, whereas saccharides of mr greater than 342 did not. cells treated with 0.5 m saccharides of mr greater than 342 weighed 33 to 38% less than cells treated with isotonic solution, suggesting that these saccharides do not permeate through the outer membrane. the ...19882835003
photophysics of metalloazurins.the fluorescence lifetimes of cu(ii), cu(i), ag(i), hg(ii), co(ii), and ni(ii) azurin pae from pseudomonas aeruginosa and cu(ii), cu(i), and hg(ii) azurin afe from alcaligenes faecalis were measured at 295 k by time-correlated single-photon counting. in addition, fluorescence lifetimes of cu(ii) azurin pae were measured between 30 and 160 k and showed little change in value. ultraviolet absorption difference spectra between metalloazurin pae and apoazurin pae were measured, as were the fluoresce ...19902119804
growth inhibition of selected food-borne bacteria, particularly listeria monocytogenes, by plant extracts.six extracts from chinese medicinal plants: tin men chu, sey lau pai, siu mao heung, bak tao yung, kam chin chiu and liao ya, were tested for their inhibitory effect on selected food-borne bacteria by the well assay technique. among them, tin men chu, siu mao heung and sey lau pai inhibited the growth of staphylococcus aureus, klebsiella pneumonia, escherichia coli, shigella flexneri, streptococcus faecalis, salmonella paratyphi, salm. enteritidis, enterobacter aerogenes, pseudomonas fluorescens ...19902127264
an examination of proton translocation and energy conservation during heterotrophic nitrification.whether selected heterotrophic nitrifiers, as do the autotrophs, conserve energy during the oxidation of their nitrogenous substrates was studied. the examination of proton translocation of four different bacterial nitrifiers capable of pyruvic oxime [(po), ch3-c(noh)-cooh] nitrification and by an nh4+ oxidizing arthrobacter sp. was initiated. three of the po nitrifying bacteria, all pseudomonads, oxidize hydroxylamine (nh2oh) at a greater rate than po and yielded only stoichiometric protons whe ...19902157622
a novel nitrilase, arylacetonitrilase, of alcaligenes faecalis jm3. purification and characterization.a new type of nitrilase, arylacetonitrilase, has been purified from isovaleronitrile-induced cells of alcaligenes faecalis jm3 in four steps. the purity of the enzyme was confirmed by sds/polyacrylamide gel electrophoresis, ampholyte electrofocusing and double immunodiffusion in agarose. the enzyme has a molecular mass of about 275 kda and consists of six subunits of identical molecular mass. the purified enzyme exhibits a ph optimum of 7.5 and a temperature optimum of 45 degrees c. the enzyme i ...19902269298
curdlan, a (1----3)-beta-d-glucan from alcaligenes faecalis var. myxogenes ifo13140, activates the alternative complement pathway by heat treatment.from the results of consumption experiments of guinea pig complement, curdlan, a (1----3)-beta-d-glucan obtained from alcaligenes faecalis var. myxogenes ifo13140, has been found to lack the ability to activate complement when unheated or preheated at either 40 degrees c or 50 degrees c. however, curdlan heated at or above 60 degrees c increased complement consumption. this activation, dependent on the temperature of the curdlan, was via the alternative complement pathway as assessed by cleavage ...19902276766
microbial metabolism of quinoline and related compounds. iv. degradation of isoquinoline by alcaligenes faecalis pa and pseudomonas diminuta 7.from sewage and soil isoquinoline-degrading organisms were enriched. two strains could be isolated which were able to utilize isoquinoline as sole carbon source. the bacteria were tentatively identified as alcaligenes faecalis and pseudomonas diminuta with respect to their morphological and physiological characters. when growing on isoquinoline both strains excrete a metabolite into the medium which was identified as 1-oxo-1,2-dihydroisoquinoline. alcaligenes faecalis was cultivated in continuou ...19902390217
quantitative selection of denitrifying bacteria in continuous cultures and requirement for organic carbon. ii. maltose.a mixed population of bacteria from industrial nitrogen wastewaters was incubated in continuous culture in medium containing 1000-1300 mg nitrate nitrogen per litre and maltose as a source of organic carbon. maximal efficiency of denitrification was 8.6 mg n/1/h. the participation of denitrifying bacteria in the culture varied from 0% to about 80%, depending on the ratio of maltose concentration (cm) to nitrogen concentration (cn) in the medium. the optimal cm/cn ratio ensuring the greatest sele ...19852421546
[antigenic polysaccharides of bacteria. 19. the structure of the o-specific polysaccharide chain of alcaligenes faecalis lipopolysaccharide].on mild acid hydrolysis of alcaligenes faecalis lipopolysaccharide, the o-specific polysaccharide containing d-rhamnose and d-xylose in the 3:2 ratio was obtained. solvolysis of the polysaccharide with hydrogen fluoride in methanol resulted in methyl glycoside of a branched tetrasaccharide including three rhamnose and one xylose residues. smith degradation of the polysaccharide led to the glycoside of disaccharide composed of two rhamnose residues and glycerol. on the basis of identification of ...19862432903
antibacterial effect of imipenem in vitro against important aerobic and anaerobic strains isolated from clinical specimens.imipenem is a thienamycin antibiotic of the first generation with broad antibacterial activity. it covers all gram-positive organisms (including streptococcus faecalis) and gram-negative bacteria (including pseudomonas aeruginosa and serratia spp.) as well as bacteroides fragilis and other bacteroides species. in this comparative study the antimicrobic effect against 1020 gram-negative, 927 gram-positive and 352 anaerobic strains from fresh clinical isolates was tested and compared with that of ...19873477332
[physico-chemical properties and heterogeneity of alcaligenes faecalis polysaccharide].two species of o-antigenic molecules with following sedimentation characteristics s(o)20, w 1,25.10(-13) s, d(o)20, w 9,7.10(-7) cm2/s, m 8000 and s(o)20, w 2,5.10(-13) s, d(o)20, w 5.10(-7) cm2/s, m 23,000-30,000 were detected in the cell wall of the strain alcaligenes faecalis, a representative species of conditionally pathogenic microorganisms with unidentified taxonomic position. "light" and "heavy" types of molecules have a lipopolysaccharide nature and show no differences in the monosaccha ...19862432904
plasmid dna of virulent alcaligenes faecalis.alcaligenes faecalis strains originating from chickens and from epizootics of coryza in turkeys were screened for antibiotic susceptibility and for the presence of plasmid dna. seven of 35 strains contained plasmid dna ranging in size from 10.5 to approximately 32 megadaltons. all of the strains isolated from turkeys were virulent in turkey poults, but only the plasmid-containing strains were resistant to sulfonamides and streptomycin. four of the plasmid-containing strains were also resistant t ...19863008603
isolation and characterization of a penicillinase from pseudomonas cepacia 249.pseudomonas cepacia has an inducible beta-lactamase which is responsible for its novel ability to catabolize beta-lactam compounds. the gene encoding this enzyme, pena, was cloned from a genomic library of p. cepacia 249 on the broad-host-range cosmid plafr. this separated the pena gene from the gene encoding a second beta-lactamase in p. cepacia 249. expression of pena was inducible in an escherichia coli host strain by low levels of penicillin. the 33,500-molecular-weight enzyme had penicillin ...19883046483
a note on the isoprenoid quinone content of bordetella avium and related species.the isoprenoid quinone content of isolates of bordetella avium (four strains), alcaligenes faecalis (one strain), bordetella bronchiseptica (one strain) and a bordetella avium-like organism (four strains) was determined by reverse-phase high-performance liquid chromatography. all the isolates contained ubiquinones with eight isoprene units as the major component. no menaquinones were detected.19873597205
differentiation of bordetella avium and related species by cellular fatty acid analysis.the fatty acids of 18 strains of bordetella avium, 3 strains of alcaligenes faecalis, 5 strains of bordetella bronchiseptica, and 12 strains of a b. avium-like organism were examined by gas chromatography-mass spectrometry. the presence of a significant amount of the acid 2-oh c14:0 characterized b. avium and the b. avium-like organism. b. avium and the b. avium-like organism differed in their relative concentrations of c16:1 and 3-oh c14:0 acids. b. bronchiseptica and a. faecalis were distingui ...19873597749
calcium-dependent and -independent tumoricidal activities of polymorphonuclear leukocytes induced by a linear beta-1,3-d-glucan and phorbol myristate acetate in mice.some antitumor immunomodulators, such as a linear beta-1,3-d-glucan from alcaligenes faecalis var. myxogenes ifo 13140 (tak), induce potent tumoricidal activity of polymorphonuclear leukocytes (pmns). in the present study we investigated the role of calcium on the tumoricidal activity of pmns induced by immunomodulators, especially tak. the calcium chelator ethylene glycol bis(beta-aminoethyl ether)-n,n,n',n'-tetraacetic acid (egta) almost completely inhibited tak-induced pmn cytotoxicity and th ...19863079591
[aerobic bacterial flora of the nasal cavity of rabbits].on the basis of bacteriological examinations carried out in april 1984 on 60 intranasal swabs, aerobic respiratory microbes were studied in rabbits. differences in flora between animals with and without respiratory diseases were studied. fourteen bacterial species were identified with no difference due to the pathological status. they were: bordetella bronchiseptica, staphylococcus epidermidis, streptococcus faecalis, pasteurella multocida, staphylococcus aureus, bacillus sp., branhamella catarr ...19863096189
the purification and characterization of a beta-glucosidase from alcaligenes faecalis.the beta-glucosidase from alcaligenes faecalis has been purified to homogeneity (880-fold purification, 11% yield) using a combination of classical techniques and medium pressure ion-exchange chromatography. it is a dimeric enzyme of monomer molecular weight 50,000 and has no specific requirement for divalent metal ions. it has a high specificity for beta-glucosides and hydrolyses a wide variety of different chemical types wit retention of configuration at the anomeric centre. it has no exo-beta ...19863096349
neplanocin a inhibition of s-adenosylhomocysteine hydrolase in alcaligenes faecalis has no effect on growth of the microorganism.neplanocin a, a cyclopentenyl analog of adenosine, is a naturally occurring antibiotic possessing potent inhibitory activity toward the enzyme s-adenosylhomocysteine (adohcy) hydrolase. in the present study, we examined whether there was a correlation between the inhibition of prokaryotic adohcy hydrolase and the reported antibacterial activity of neplanocin a, e.g. alcaligenes faecalis (yaginuma et al., j. antibiotics 34: 359 approximately 366, 1981). of 16 bacterial species screened, only 2 or ...19873112082
induction of tumoricidal activity of polymorphonuclear leukocytes by a linear beta-1,3-d-glucan and other immunomodulators in murine cells.the cytotoxic activity of polymorphonuclear leukocytes (pmn) against tumor cells induced in vitro by antitumor immunomodulators was examined by a 51cr release cytotoxicity assay. among 28 immunomodulators and other agents thus far tested, only beta-1,3-glucan from alcaligenes faecalis var. myxogenes ifo 13140, bacillus calmette-guérin, propionibacterium acnes, zymosan a, and nocardia cell wall skeleton were found to cause induction. the cytotoxic activity of pmn with the beta-1,3-glucan was very ...19853156669
the blue copper protein gene of alcaligenes faecalis s-6 directs secretion of blue copper protein from escherichia coli cells.the gene encoding a blue copper protein (a member of the pseudoazurins) of 123 amino acid residues, containing a single type i cu2+ ion, was cloned from alcaligenes faecalis s-6. the nucleotide sequence of the coding region, as well as the 5'- and 3'-flanking regions, was determined. the deduced amino acid sequence after glu-24 coincided with the reported sequence of the blue protein, and its nh2-terminal sequence of 23 residues resembled a typical signal peptide. the cloned gene was expressed u ...19872824441
comparative in-vitro activity of ciprofloxacin against non-fermenters.the in-vitro activity of ciprofloxacin, a quinolone-carboxylic acid derivative, was compared with those of carbenicillin, azlocillin, cefsulodin, ceftazidime, tobramycin and amikacin against 187 non-fermenters. only one of the 131 strains of pseudomonas spp. was not inhibited by 1 mg/l of ciprofloxacin, while these isolates appeared highly resistant to carbenicillin, azlocillin and cefsulodin. ciprofloxacin was also the best agent against flavobacterium, alcaligenes faecalis and acinetobacter ca ...19853159710
nitric oxide complex of cytochrome c' in cells of denitrifying bacteria.electron paramagnetic resonance (epr) spectra at 77k have been measured for the dried cells of three groups of chemoheterotrophic bacteria cultured in the presence of nitrate: i, denitrifying bacteria containing cytochrome c' (alcaligenes sp. ncib 11015, achromobacter xylosoxidans gifu 543); ii, denitrifying bacteria in which cytochrome c' has not been found (alcaligenes faecalis iam 1015, bacillus firmus nias 237, pseudomonas stutzeri atcc 17641); iii, non-denitrifying bacterium containing cyto ...19882844743
electrophoretic microheterogeneity and subunit composition of the 13s coupling factors of oxidative and photosynthetic phosphorylation.two electrophoretically distinguishable species of the 13s coupling factor of oxidative phosphorylation from alcaligenes faecalis are detectable by standard polyacrylamide gel electrophoresis in the absence of urea, detergents, or any other protein-denaturing reagents. the slower species (type ia) can be converted into the faster species (type ib) by treatment with atp, and the fast form converts into the slow form when aged at 4 degrees. the enzyme undergoes these conversions both when it is fr ...19751168491
antitumor activity of carboxymethylglucans obtained by carboxymethylation of (1 leads to 3)-beta-d-glucan from alcaligenes faecalis var. myxogenes ifo 13140. 1979436898
antimicrobial activities of 81.723 hfu, a new pleuromutilin derivative.the new pleuromutilin derivative 81.723 hfu is extremely active against gram-positive organisms such as streptococci, staphylococci, and against mycoplasmas. a number of shigella, klebsiella, and escherichia coli strains were also found to be quite susceptible to this new agent, whereas other gram-negative organisms like pseudomonas aeruginosa, proteus species, and alcaligenes faecalis proved to be naturally resistant to 81.723 hfu. the new compound acts bacteriostatically. bactericidal effects ...19751170807
[effect of silver preparations on the microflora regenerated from a condensate of atmospheric water vapor in a hermetically-sealed chamber].the effect of different silver compounds (silver sulphate, silver nitrate, electrolytic ionic silver) on microorganisms in water reclaimed from the atmospheric condensate in an enclosed environment was investigated. the following microorganisms were examined: alcaligenes faecalis, citrobacter freundii. aeromonas hydrophila, staphylococcus epidermidis, etc. the ionic silver concentrations in solution varied from 0.1 to 10 mg/l. the efficiency of the silver compounds was found to depend on the mic ...19872886695
[microflora of the drinking water regenerated from atmospheric moisture condensate in hermetically sealed quarters].in an enclosed environment the species composition of microorganisms was quantified and metabolic processes in microbial cells isolated from the atmospheric condensate and reclaimed water were investigated. it was demonstrated that indicator microorganisms isolated from water and condensate samples (alcaligenes faecalis, citrobacter freundii, aeromonas hydrophila, acinetobacter-moraxella) were specific and differed from indicator microorganisms typical of regular tap water.19882898035
a 2.0-a structure of the blue copper protein (cupredoxin) from alcaligenes faecalis s-6.the structure of a blue copper protein, cupredoxin, from the potent denitrifying bacterium alcaligenes faecalis s-6, has been determined and refined against 2 a x-ray diffraction data. the agreement between observed and calculated structure factors is 0.159, and estimated errors in coordinates are 0.09-0.15 a. the protein folds in a beta sandwich similar to plastocyanin and azurin and includes features such as a "kink" and a "tyrosine loop" which have been noted previously for these proteins as ...19892909547
unusual resistance to cefuroxime by strains of alcaligenes faecalis. 19892921091
[utilization of 3-chlorobenzoic acid by a mixed culture of microorganisms].a mixed microbial culture (acinetobacter calcoaceticus inmi-kz-3 and alcaligenes faecalis inmi-kz-5) completely utilises 3-chlorobenzoic acid (3-cba), and the liberation of chlorine atoms is 100% of the theoretically possible one. a. faecalis growth in the mixed culture at the account of 2-chloro-cis,cis-muconic acid which is accumulated in the course of 3-cba metabolism by a. calcoaceticus inmi-kz-3.19883211007
[antibiotic sensitivity of nonfermenting gram-negative bacteria].antibiotic resistance of 132 strains of nonfermenting gram-negative bacteria (ngnb) was studied. 43, 20, 17, 14 and 12 of them belonged to acinetobacter calcoaceticus (anitratus and lwoffi), pseudomonas cepacia, alcaligenes faecalis, p. stutzeri and p. maltophilia, respectively. with rare exceptions all the strains were resistant to benzylpenicillin, oxacillin, lincomycin, ampicillin and cephaloridine. sensitivity to the other antibiotics varied within wide ranges. amikacin (94.3 per cent) and t ...19883228322
activation of the alternative pathway of complement by an antitumor (1----3)-beta-d-glucan from alcaligenes faecalis var. myxogenes ifo 13140, and its lower molecular weight and carboxymethylated derivatives.an antitumor (1----3)-beta-d-glucan with a number-average degree of polymerization (dp) of 540 from alcaligenes faecalis var. myxogenes ifo 13140, and its lower molecular weight derivatives were found to activate the alternative pathway of complement (apc), as judged by hemolytic and immunoelectrophoretic analyses. of the native and derivative (1----3)-beta-d-glucans measured, the smallest one that showed apc-activating ability was that with a dp of about 20. the effect of carboxymethylation of ...19863957648
refinement of the structure of pseudoazurin from alcaligenes faecalis s-6 at 1.55 a resolution.the crystal structure of the redox protein pseudoazurin (123 amino acid residues; molecular weight 13,000 daltons) from alcaligenes faecalis has been refined by fast fourier restrained least-squares minimization. cycles of rebuilding were carried out to escape from local minima. individual isotropic temperature factor values were refined separately for all atoms. the r factor was reduced from 0.400 (for 2647 reflections in the 6.0-2.8 a resolution range) to 0.180 (for all 19,770 reflections in t ...19883271558
[comparative in vitro study of the antimicrobial activity of ceftazidime against clinical isolates].the antimicrobial activity of ceftazidime was tested against 1482 gram-negative and 1216 gram-positive strains isolated from fresh clinical specimens and compared with generally used antibiotics including other third generation cephalosporins and broad spectrum penicillins. minimal inhibitory concentrations were determined in a broth dilution test on microtiter plates. in the group of the gram-negative bacteria ceftazidime was the most active of the antimicrobial agents tested with an mic of 0.5 ...19873312026
effect of limited tryptic modification of a bacterial poly(3-hydroxybutyrate) depolymerase on its catalytic activity.the extracellular poly(3-hydroxybutyrate) depolymerase of alcaligenes faecalis t1, which hydrolyzes both hydrophobic poly(3-hydroxybutyrate) and water-soluble oligomers of d(-)-3-hydroxybutyrate, lost its hydrolyzing activity toward the hydrophobic substrate on mile trypsin treatment, but retained its activity toward water-soluble oligomers. the molecular mass of the trypsin-treated enzyme was 44 kda, as estimated by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate, w ...19883337823
a comparative study of the resonance raman spectra of bacterial cytochromes.resonance raman spectra were obtained for two newly isolated bacterial cytochromes, alcaligenes faecalis (atcc 8750) c554 and alcaligenes faecalis c556. their spectra were compared with those of mammalian cytochrome c and two other bacterial cytochromes, paracoccus denitrificans c550 and pseudomonas aeruginosa c551. the positions of the raman bands indicated that, while al. c554 and al. c556 were c-type cytochromes with two thioether linkages, several common features found in their raman spectra ...19852982314
hydrogen peroxide as a tumoricidal mediator of murine polymorphonuclear leukocytes induced by a linear beta-1,3-d-glucan and some other immunomodulators.polymorphonuclear leukocytes (pmn) of mice can destroy tumor cells effectively in vitro in the presence of antitumor polysaccharide, linear beta-1, 3-d-glucan from alcaligenes faecalis var. myxogenes ifo 13140 (tak), and some other immunomodulators. in the present study, we investigated the mechanism of the tumoricidal activity of pmn induced by these immunomodulators and especially tak. the tak-induced pmn cytotoxicity was concluded to involve hydrogen peroxide from the following results: (a) t ...19852990672
the crystal structure of pseudoazurin from alcaligenes faecalis s-6 determined at 2.9 a resolution.the three-dimensional structure of pseudoazurin, a single copper-containing protein from alcaligenes faecalis strain s-6, has been determined at 2.9 a resolution by x-ray crystallography. the sequences of two other pseudoazurins from pseudomonas am1 and achromobacter cycloclastes may also be accommodated in this structure. the structure, an eight-stranded beta-barrel, resembles closely those of plastocyanin and azurin. it possesses two extra alpha-helices at the c-terminus, whereas azurins have ...19873595868
the mechanism of inhibition of alcaligenes faecalis s-adenosylhomocysteine hydrolase by neplanocin a.neplanocin a, a cyclopentenyl analog of adenosine, has been reported by s. yaginuma, n. muto, m. tsujino, y. sudate, m. hayashi, and m. otari (1981) j. antibiot. 34, 359-366 to exhibit antibacterial activity against alcaligenes faecalis. since neplanocin a (npca) is a known inhibitor of eukaryotic s-adenosylhomocysteine (adohcy) hydrolase (ec 3.3.1.1) (r. t. borchardt, b. t. keller, and u. patel-thombre (1984) j. biol. chem. 259, 4353-4358), the present study was undertaken to determine the effe ...19873606132
[statistical observation on chronological changes of sensitivity of various bacteria to antibiotics. 1. gram negative bacilli; escherichia coli, bacillus pneumoniae, proteus vulgaris, alcaligenes faecalis, cloaca, providencia, citrobactor, pseudomonas aeruginosa (1966-71)]. 19734198261
isolation and characterisation of bordetella avium and related species and an evaluation of their role in respiratory disease in poultry.a total of 24 gram negative non fermentative bacteria obtained from poultry were compared with reference strains of bordetella avium, alcaligenes faecalis, bordetella bronchiseptica and a bordetella avium-like organism. thirteen isolates were identified as b. avium and 11 were identified as b. avium-like. a commercial microidentification kit (the api2one) did not identify the field isolates but did separate them correctly into the 2 groups. a practical identification scheme, suitable for diagnos ...19873689262
the efficacy of a temperature-sensitive mutant vaccine against northwest arkansas isolates of alcaligenes faecalis.the efficacy of a commercially produced temperature-sensitive mutant alcaligenes faecalis vaccine was evaluated in turkeys contact-challenged with one of three strains of a. faecalis. in the vaccinated control group, the vaccine strain of a. faecalis colonized the nasal turbinates but not the trachea, caused no clinical signs of turkey coryza, and induced humoral antibodies. in the vaccinated challenged groups, the vaccine reduced both the severity of lesions and the number of birds exhibiting l ...19863729855
tracheal mucus transport rate in normal turkeys and in turkeys infected with bordetella avium (alcaligenes faecalis).using the radiopharmaceutical 99mtechnetium-sulfur colloid, the tracheal mucus transport rate (tmtr) was measured in healthy unanesthetized turkeys and in turkeys infected with bordetella avium. the tmtr of uninfected turkeys was 35.6 +/- 14.4 cm/min. the tmtr of b. avium-infected turkeys was normal on days 0 through 14 postexposure (pe), despite heavy bacterial colonization of the tracheal epithelium. on day 21 pe, the tmtr of b. avium-infected turkeys was significantly depressed (p less than o ...19863729858
contribution to the taxonomy of the turkey coryza agent: cellular fatty acid analysis of the bacterium.gas-liquid chromatography was used to analyze bacterial cellular fatty acids to elucidate the relatedness of the turkey coryza (tc) bacterium to alcaligenes spp., bordetella spp., and other gram-negative bacteria. the results indicated that the tc bacterium is not closely related to alcaligenes faecalis or any of the reference strains of alcaligenes and bordetella studied. most urease-positive bacterial isolates obtained from the upper respiratory tract of turkeys were identified as bordetella b ...19863729861
an evaluation of two methods of substrate alkalinization for the identification of bordetella avium and other similar organisms.the effect of using two different techniques for the detection of substrate alkalinization by bordetella avium and reference strains of alcaligenes faecalis and b. bronchiseptica was evaluated. the techniques used were those described by otto and pickett and hinz et al. each test was in tubes sealed by either screw caps or cotton wool plugs. the alkalinization patterns obtained depended upon both the technique and the type of seal used.19863739213
survey of disc diffusion antimicrobial sensitivity testing in avian bacteriology laboratories and the evaluation of a standardised method.a survey was conducted in which 15 laboratories involved in avian bacteriology tested the antimicrobial sensitivity of cultures of staphylococcus aureus, s. epidermidis, escherichia coli, alcaligenes faecalis and salmonella typhimurium, using the disc diffusion test as routinely practised in each laboratory. up to 28 different antimicrobials or antimicrobial combinations were used. the most commonly tested agents were ampicillin, benzylpenicillin, chloramphenicol, erythromycin, furazolidone, neo ...19863741274
preliminary characterization of crystals of nitrite reductase isolated from alcaligenes faecalis strain s-6.crystals of the green, copper-containing enzyme nitrite reductase from alcaligenes faecalis s-6 have been obtained. they are monoclinic, space group p2(1) with a = 78.0 a, b = 92.9 a, c = 78.5 a and beta = 111.8 degrees. the crystals diffract to better than 2 a and are suitable for high-resolution x-ray crystallography.19863783698
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