| investigations of the structure of 3-methylcrotonyl-coa carboxylase from achromobacter. | it was shown by gel electrophoresis in sodium dodecylsulphate solution that 3-methylcrotonyl-coa carboxylase from achromobacter ivs is composed of two different subunits with molecular weights of about 78000 and 96000, respectively. the biotin is bound to the heavier subunit. it was previously found that 3-methylcrotonyl-coa carboxylase contains four biotin molecules per complex. a complex composed of four of each subunit would thus have a molecular weight of about 700000. this is compatible wit ... | 1975 | 1267 |
| the effect of bile acids and their salts on the lipolytic activity of microorganisms. | | 1975 | 2339 |
| aromatic amino acid biosynthesis in alcaligenes eutrophus h 16 iii. properites and regulation of anthranilate synthase. | properties and regulation of anthranilate synthase from alcaligenes eutrophus h 16 were investigated. anthranilate synthase was partially purified from crude extracts by affinity chromatography on tryptophan-substituted sepharose, and was used for kinetic measurements. during the purification procedure the enzyme was stabilized by 50 mm l-glutamine or during chromatography on deae- cellulose and sephadex g-200 with 30% glucerol, respectively. | 1976 | 4044 |
| determination of d-3-hydroxybutyrate dehydrogenase in mouse pancreatic islets with a photokinetic technique using bacterial luciferase. | a sensitive assay for d-3hydroxybutrate dehydrogenase (ec 1.1.1.30) was developed for use with the minute amounts of material obtained from islets of langerhans microdissected from freeze-dried pancreatic sections. nadh formed in the enzyme reaction was determined by photokinetic analysis of the luminescence obtained with bacterial luciferase from achromobacter fishcherii. in this way, accurate determination was obtained with less than 0.1 mug dry weight of islet material. in obese hyperglycemic ... | 1976 | 4298 |
| regulation of chorismate mutase-prephenate dehydratase and prephenate dehydrogenase from alcaligenes eutrophus. | highly purified enzymes from alcaligenes eutrophus h 16 were used for kinetic studies. chorismate mutase was feedback inhibited by phenylalanine. in the absence of the inhibitor, the double-reciprocal plot was linear, yielding a km for chorismate of 0.2 mm. when phenylalanine was present, a pronounced deviation from the michaelis-menten hyperbola occurred. the hill coefficient (n) was 1.7, and hill plots of velocity versus inhibitor concentrations resulted in a value of n' = 2.3, indicating posi ... | 1976 | 4432 |
| purification, stability and inhibition of the collagenase from achromobacter iophagus. | | 1975 | 6314 |
| molecular polymorphism and mechanisms of activation and deactivation of the hydrolytic function of the coupling factor of oxidative phosphorylation. | the 13s coupling factor of oxidative phosphorylation from alcaligenes faecalis has a latent adenosine triphosphatase (atpase) function that can be activated by heating at 55 degrees c for 10 min at ph 8.5 in 50% glycerol. the specific activity increases from 0.1 to 20--30 mumol min-1 mg-1. adenosine 5'-triphosphate (atp) is not required for stabilization at 55 degreesc when glycerol is present. activation involves displacement of the endogenous atpase inhibitor subunit (epsilon subunit), and rea ... | 1976 | 9131 |
| oxidation of arsenite to arsenate by alcaligenes faecalis. | alcaligenes faecalis, resistant to the toxic effects of 0.01 m sodium arsenite, was isolated from raw sewage and shown to be capable of oxidizing arsenite to arsenate. when the organisms were grown in chemically defined medium, this conversion was due to the appearance at stationary phase of an intracellular, oxygen-sensitive, inducible enzyme and/or component of the electron transport system; when the organisms were grown in a nutrient broth-yeast extract medium, the enzyme appeared in the late ... | 1976 | 10837 |
| dense autotrophic cultures of alcaligenes eutrophus. | alcaligenes eutrophus was grown autotrophically in 23-liter batch cultures in a controlled h2-o2-co2 atmosphere. it was demonstrated that the need for periodic supplements of individual nutrients could be anticipated before cell growth depleted these nutrients to the point of becoming growth rate limiting. as a result, exponential growth was extended to optical densities of 44, with doubling times maintained at 2 h. cultures having an initial optical density of 0.040 to 0.70 reached the final op ... | 1976 | 10840 |
| biosynthesis of chitinase by achromobacter liquefaciens. | bacterial cultures under study synthesize exocellular chitinase on a medium containing chitin or demineralized crab shells as a source of carbon and nitrogen. conditions for biosynthesis of chitinase by the cells of achromobacter liquefaciens 301a were investigated under periodic and continuous conditions of cultivation. the preparation of chitinase isolated from the cultural broth of a. liquefaciens 301a hydrolysed colloid and native chitin at the optimum ph 6.5 and temperature 40degreesc. the ... | 1976 | 12452 |
| bacteriolysis by immobilized enzymes. | bacteriolytic enzymes produced by achromobacter lunatus were immobilized in collagen membrane. intact bacteria such as pseudomonas solanacearum, xanthomonas oryzae, staphylococcus aureus, and pseudomonas aeruginosa were lyzed with the bacteriolytic enzyme-collagen membrane. relative activity of the bacteriolytic enzyme-collagen membrane against pseu. solanacearum was about 2% of that of native bacteriolytic enzymes. no difference in the optimum ph was observed between immobilized enzymes and nat ... | 1977 | 14747 |
| alpha-isopropylmalate synthase from alcaligenes eutrophus h 16 i. purification and general properties. | alpha-isopropylmalate (ipm) synthase, the first enzyme in the biosynthesis of l-leucine, was purified to a specific activity of 12 micronmole/min x mg protein from the valine-isoleucine double auxotrophic mutant a-81 of the hydrogen bacterium alcaligenes eutrophus h16. the activity in crude extracts of derepressed cells was 0.106 micronmoles of isopropylmalate formed per min and per mg protein. gel electrophoresis and regel electrophoresis of the isolated main band resulted in several distinct b ... | 1977 | 16576 |
| alpha-isopropylmalate synthase from alcaligenes eutrophus h 16. iii. endproduct inhibition and its relief by valine and isoleucine. | the alpha-isopropylmalate synthase (ec 4.1.3.12) from alcaligenes eutrophus h 16 was inhibited by l-leucine and alpha-ketoisocaproate. the extent of inhibition was influenced by substrate- and inhibitor concentrations as well as by the ph. intermediary plateaus, which always appeared in the inhibition curves, suggested cooperative effects. the maximal hill coefficient was found to be two. at low concentrations of leucine the inhibition mechanism was of the competitive type with respect to substr ... | 1977 | 20865 |
| continuous production of nadp by immobilized achromobacter aceris cells. | several microorganisms having higher nicotinamide adenine dinucleotide kinase (nad kinase, ec 2.7.1.23) activity were immobilized into polyacrylamide gel lattices. the enzyme activity field by immobilization was highest in achromobacter aceris aku 0120. by the incubation of the immobilized a. aceris cells at ph 4.0, the nad kinase activity increased and the adenosine triphosphate (atp)-degradation activity disappeared completely. enzymatic properties of the immobilized a. aceris cells were inves ... | 1978 | 24483 |
| new trends in cryoenzymology: i.-supercooled aqueous solutions. | a water in oil emulsion technique is proposed to investigate enzyme catalyzed reactions at sub-zero temperatures in the supercooled liquid state to avoid some reversible effects of the usual cosolvents on kinetics. some results are listed: potentialities and technical problems of the procedure are discussed. | 1978 | 27242 |
| mn++-specific reactivation of edta inactivated alpha-isopropylmalate synthase from alcaligenes eutrophus h 16. | | 1978 | 29612 |
| studies of relationship among terrestrial pseudomonas, alcaligenes, and enterobacteria by an immunological comparison of glutamine synthetase. | antibody to purified glutamine synthetase from escherichia coli was prepared and used for an immunological comparison of glutamine synthetases from species of salmonella, citrobacter, enterobacter, serratia, proteus, erwinia, aeromonas, pseudomonas, acinetobacter, xanthomonas, alcaligenes, and paracoccus. the results of ouchterlony double diffusion experiments and quantitative microcomplement fixation studies indicated that the amino acid sequence of this enzyme was highly conserved in different ... | 1978 | 31146 |
| precipitate produced by serratia marcescens on macconkey agar: useful diagnostic test. | the production of a precipitate by serratia marcescens on oxoid macconkey agar has proven useful as a laboratory diagnostic test. this phenomenon is specific for serratia within the enterobacteriaceae, although precipitate production is also given by acinetobacter anitratus and some pseudomonas, alcaligenes, and aeromonas species. precipitate production seems to be specific for certain batches of macconkey agar, and is probably related to a specific property of some batches of bile salts. | 1978 | 32188 |
| [isolation, purification and study of the stability of the soluble hydrogenase of alcaligenes eutrophus z-1]. | soluble hydrogenase was isolated from the hydrogen-oxidizing bacterium alcaligenes eutrophus z-1 and purified to electrophoretical homogeneity. the purification procedure included fractionation by ammonium sulfate, ion-exchange chromatography on deae-cellulose and gelfiltration through ultragel aca-34. the resulting preparation had a specific activity of 25 mkmoles h2.min-1.mg of protein as measured by the rate of hydrogen evolution from sodium dithionite-reduced methyl viologen. the enzyme has ... | 1979 | 35246 |
| the membrane-bound hydrogenase of alcaligenes eutrophus. i. solubilization, purification, and biochemical properties. | the membrane-bound hydrogenase of alcaligenes eutrophus was solubilized from washed membranes of autotrophically grown cells. the enzyme consists of two types of subunits and is an iron-sulfur protein. a flavin compound was not detected. the enzyme reacts only with few artificial electron acceptors. | 1979 | 36155 |
| isolation and properties of a particulate fraction for the desaturation of palmitic acid from alcaligenes faecalis. | a particulate fraction obtained from alcaligenes faecalis could desaturate palmitic acid to palmitoleic acid. nadph, atp, coa, fe2+ and mg2+ were essential cofactors for the reaction. the desaturation showed an absolute requirement for o2. metal ions like mn2+, mo6+ and cu2+ did not affect the desaturation, while zn2+ was inhibitory. sulfhydryl agents such as cysteine, glutathione and beta-mercaptoethanol had no effect, but sh-blocking agents like hgcl2 and p-hydroxymercuribenzoate inhibited the ... | 1979 | 39619 |
| [calcifying bacteria and carbonic anhydrase]. | carbonic anhydrase activity was measured on bacteria known for their calcifying power. the results obtained allow us to conclude that carbonic anhydrase is not present in their enzymatic equipment. nevertheless, the addition of carbonic anhydrase in culture media increases the growth of the cultures and their calcifying power. this result is due to the direct action of the enzyme on the ph of the culture medium. the results obtained could be applied to the mechanism of dental plaque calcificatio ... | 1979 | 39682 |
| new experiments of biotin enzymes. | the objects of structural studies on biotin-enzymes were acetyl coa-carboxylase and pyruvate carboxylase of saccharomyces cerevisiae and beta-methylcrotonyl coa-carboxylase and acetyl coa-carboxylase of achromobacter iv s. it was found that these enzymes can be arranged in three groups. in the first group, as represented by acetyl coa-carboxylase of achromobacter, the active enzyme could be resolved in three types of functional components: (1) the biotin-carboxyl carrier protein, (2) the biotin ... | 1979 | 41682 |
| a selective medium facilitating the isolation and recognition of bordetella bronchiseptica in pigs. | a medium was developed that in most instances allowed the isolation from pigs of bordetella (alcaligenes) bronchiseptica in pure, or virtually pure, culture from such sites as the nasal cavity, which contains many other bacteria. if not suppressed, some of the latter can inhibit, sometimes completely, the growth of the bordetellae on artificial media. besides being markedly selective, the new medium is simple to prepare, reasonably cheap and practically noninhibitory to b bronchiseptica. this or ... | 1979 | 42958 |
| catechol 1,2-dioxygenase from acinetobacter calcoaceticus: purification and properties. | procedures for the purification of catechol 1,2-dioxygenase from extracts of acinetobacter calcoaceticus strain adp-96 are described. the purified enzyme was homogeneous as judged by ultracentrifugation and acrylamide gel electrophoresis. the enzyme contained 2 g-atoms of iron per mol of protein. the enzyme had a broad substrate specificity and catalyzed the oxidation of catechol, 4-methylcatechol, 3-methylcatechol, and 3-isopropyl catechol. the activity of the enzyme was inhibited by heavy meta ... | 1976 | 58860 |
| studies on the biodegradation of nonionic surfactants applied in the polyester fiber industry. i. activated sludge bacteria degrading the surfactants. | the paper presents characteristics of 76 strains of bacteria capable of utilizing nonionic surfactants cirrasol fp, cirrasol sf 200 and cirrasol tcs as the source of carbon. the strains were isolated from two activated sludges adapted to the purification of wastes containing the above compounds at concentration 150--200 mg/l. the isolated strains belonged to the genera: achromobacter, alcaligenes, arthrobacter, flavobacterium, mycobacterium, nocardia, pseudomonas and xanthomonas. with load 0.11 ... | 1976 | 62497 |
| amikacin, an aminoglycoside with marked activity against antibiotic-resistant clinical isolates. | a total of 319 clinical isolates known to be resistant to one or more aminoglycoside antibiotics were tested for their susceptibility to 10 aminoglycosides. the percentages of isolates found by an agar dilution method to be susceptible were: amikacin, 83.7%; tobramycin, 41.4%; butirosin a, 33.2%; dideoxykanamycin b, 32.6%; gentamicin c, 27.3%; lividomycin a, 17.6%; neomycin b, 10.7%; paromomycin, 10.3%; kanamycin a, 10.0%; and ribostamycin, 7.2%. the effectiveness of the antibiotics was related ... | 1976 | 62814 |
| simplified silver-plating stain for flagella. | rhodes' silver-plating technique for staining flagella was tested for its reliability and convenience as a routine procedure in the clinical laboratory. modifications were made in the stain preparation and the procedure of staining and were tested with smears of known motile gram-negative nonfermentative bacilli. the stain has proved to be accurate and reliable and can be easily utilized with a minimum of training. | 1977 | 72075 |
| [polysaccharides of microorganisms]. | | 1978 | 78432 |
| the effect of cyclohexane derivatives on selection of bacterial groups forming activated sludge microflora. | the effect of cyclohexanol, cyclohekxanon and cyclohexylamine on the selection of bacteria in a model population composed of bacteria isolated from activated sludge was examined. the initial population consisted of both gram-positive and gram-negative bacteria. the latter, which accounted for 90-97% of the population, belonged mainly to three pseudomonas groups and the enterobacteriaceae, vibrio-aeromonas, achromobacter-alcaligenes and flavobacterium groups. seven day growth in medium containing ... | 1978 | 80928 |
| studies on regulatory functions of malic enzymes. v. comparative studies of malic enzymes in bacteria. | screening of four malic enzymes--nad-linked enzyme [ec 1.1.1.38], nad, nadp-linked enzyme [ec 1.1.1.39], nadp-linked enzyme [ec 1.1.1.40], and d-malic enzyme--was carried out with cell-free extracts of the following 16 strains of bacteria by the aid of sepharose 6b column chromatography: 9 strains of enteric bacteria, 3 strains of pseudomonas, alcaligenes faecalis, agrobacterium tumefaciens, rhodospirillum rubrum, and clostridium tetanomorphum. all the strains tested contained at least one malic ... | 1978 | 96110 |
| [biological assays of the quality of protein from h2-oxidizing bacteria in rats]. | | 1978 | 99907 |
| [contamination of ultrasonic equipment for the hand disinfection by chlorhexidine-resistant alcaligenes faecalis (author's transl)]. | | 1978 | 103976 |
| catalytic mutants of ribulose bisphosphate carboxylase/oxygenase. | | 1978 | 106837 |
| degradation of polychlorinated biphenyls by mixed microbial cultures. | three different enriched mixed cultures capable of degrading polychlorinated biphenylas were isolated from two soil samples and a river sediment, respectively. the predominant organisms found in all three mixed cultures were alcaligenes odorans, alcaligenes dentrificans, and an unidentified bacterium. the polychlorinated biphenyl isomers that were more water soluble and had lower chlorination were not only degraded at a faster rate than those that were less water soluble and had higher chlorinat ... | 1979 | 110265 |
| [products of bacterial destruction of sodium dodecyl sulphate]. | the products of na dodecyl sulphate destruction by the three bacterial cultures--pseudomonas aeruginosa, flavobacterium devorans, achromobacter guttatus--were examined. the cultures were shown to decompose na dodecyl sulphate in a similar way. the primary mechanism of destruction was found to be hydrolysis of the sulpho-ester bond in the molecule, leading to the separation of sulphate-ion and formation of dodecanol. products of bacterial destruction of alkyl sulphates did not show foam forming c ... | 1979 | 117448 |
| effect of chlorine substitution on the bacterial metabolism of various polychlorinated biphenyls. | of 36 pure isomers (chlorine numbers 1 to 5) of polychlorinated biphenyls examined, 23 compounds were metabolized by alcaligenes sp. strain y42, and 33 compounds were metabolized by acinetobacter sp. strain p6. the major pathway of many polychlorinated biphenyl isomers examined was considered to proceed through 2',3'-dihydro-2',3'-diol compounds, concomitant dehydrogenated 2',3'-dihydroxy compounds, subsequently the 1',2'-meta-cleavage compounds (chlorinated derivatives of 2-hydroxy-6-oxo-6-phen ... | 1979 | 117752 |
| magnetic susceptibility of microorganisms. | total magnetic susceptibility of 13 species and varieties of bacteria was investigated using the relative method of guy. it has been established that the index of magnetic susceptibility is a constant characteristic of bacteria. total magnetic susceptibility ranged from --0.3295.10(-6) in escherichia p678 to --0.4965.10(-6) in proteus. it has also been established that magnetic susceptibility changes during long-term passages of bacteria in fluctuating +/- 0.1 oe) magnetic field. this is suggest ... | 1979 | 118995 |
| bacteriology of rattlesnake venom and implications for therapy. | although the incidence of infection secondary to the bites of venomous snakes remains unknown, the routine use of prophylactic antimicrobial therapy is advocated. in this study, the venom from 15 rattlesnakes was cultured, and 58 aerobic and 28 anaerobic strains of bacteria were isolated. the most common species isolated were pseudomonas aeruginosa, proteus species, coagulase-negative staphylocci, and clostridium species. bacteroides fragilis was also recovered. when the fang sheaths of four add ... | 1979 | 119002 |
| [drug sensitivity of glucose-nonfermenting bacteria other than pseudomonas aeruginosa (author's transl)]. | | 1979 | 119072 |
| cationic composition of 22 species of bacteria grown in seawater medium. | twenty-two species of bacteria of marine, estuarine, and terrestrial origin were analyzed for cationic content by atomic absorption spectrophotometry after growth in a basal seawater medium. alcaligenes marinus was analyzed from eight separate but replicate determinations yielding the following cationic concentrations: na, 5,600 +/- 2,260; mg 1,580 +/- 740; k, 700 +/- 360; ca, 790 +/- 390; mn, 1.7 +/- 0.5; fe, 256 +/- 57; ni, 1.7 +/- 0.7; cu, 14 +/- 4; zn, 122 +/- 27; cd, 2.8 +/- 0.7; and pb, 10 ... | 1979 | 120696 |
| over-production of porphyrins and heme in heterotrophic bacteria. | in escherichia coli, pseudomonas aeruginosa, and achromobacter metalcaligenes gamma-aminolevulinic acid synthase and gamma-aminolevulinic acid dehydratase appear to be the rate limiting enzymes of porphyrin and heme biosynthesis. bypassing of these enzymes by addition of gamma-aminolevulinic acid or porphobilinogen results in overproduction of tetrapyrroles. | 1975 | 126586 |
| pathways of d-fructose and d-glucose catabolism in marine species of alcaligenes, pseudomonas marina, and alteromonas communis. | cell-free extracts of d-fructose grown cells of marine species of alcaligenes as well as pseudomonas marina contained an activity which catalyzed a p-enolpyruvate-dependent phosphorylation of d-fructose in the 1-position as well as activities of the following enzymes: 1-p-fructokinase, fructose-1,6-p2 aldolase, ppi-dependent 6-p-fructokinase, fructokinase, glucokinase, p-hexose isomerase, glucose-6-p dehydrogenase, 6-p-gluconate dehydrase, and 2-keto-3-deoxy-6-p-gluconate aldolase. the presence ... | 1977 | 139858 |
| an adenosine triphosphate-dependent deoxyribonuclease from alcaligenes faecalis. | a deoxyribonuclease was purified approx. 800-fold from crude extracts of the bacterium alcaligenes faecalis. the enzyme requires atp and mn2+; atp could be replaced by any other ribo- or deoxyribo-nucleoside triphosphate, and mn2+ could be replaced by mg2+ in 0.1 m-tris/hcl, ph 8.0 at 37 degrees c. the enzyme could degrade linear duplex or denaturated dna, but was inactive with closed-circular duplex dna from bacteriophase pm-2. in the course of nucleolytic activity, atp was hydrolysed. we have ... | 1977 | 141925 |
| monovalent cations in mitochondrial oxidative phosphorylation. | | 1977 | 142085 |
| kinetic mechanisms of ionic activation and inhibition of the adenosine triphosphatase of the 13 s coupling factor of oxidative phosphorylation. | | 1978 | 149129 |
| the properties of adenosine triphosphatase from exponential and synchronous cultures of alcaligenes eutrophus h16. | the properties of alcaligenes eutrophus atpase (adenosine triphosphatase) were investigated by using subcellular fractions prepared from cells growing in exponential and synchronous cultures. both the soluble and membrane-bound forms of the atpase were inhibited non-competitively (k(i) 142mum) by nbf-cl (4-chloro-7-nitrobenzofurazan), whereas only the membrane-bound enzyme was inhibited (non-competitive; k(i) 750mum) by nn'-dicyclohexylcarbodi-imide. neither the activity of the atpase nor its se ... | 1978 | 149538 |
| identification of nonfermentative gram-negative bacteria in the clinical laboratory. | a simplified, concise scheme was developed for the identification of nonfermentative, gram-negative bacteria which have most frequently been reported in the literature as definite or possible agents of human disease. these organisms included apyocyanogenic pseudomonas aeruginosa, p. fluorescens, p. putida, p. stutzeri, p. maltophilia, p. putrefaciens, p. cepacia, p. alcaligenes, flavobacterium species, bordetella bronchiseptica, acinetobacter anitratum (herellea vaginicola), a. iwoffi (mima poly ... | 1975 | 163060 |
| microbial assimilation of hydrocarbons. i. the fine-structure of a hydrocarbon oxidizing acinetobacter sp. | 1. the fine-structure analysis of the hydrocarbon oxidizing microorganism, acinetobacter sp., demonstrated a cytoplasmic modification resulting from growth on paraffinic and olefinic hydrocarbons. 2. intracytoplasmic hydrocarbon inclusions were documented by electron microscopy with chemical identifications obtained by gas chromatography and x-ray diffraction. 3. these results demonstrate the ability of a microorganism to accumulate hydrocarbon substrates intracellularly which, in turn, indicate ... | 1975 | 163624 |
| collagenase production by achromobacter iophagus. | achromobacter iophagus produced collagenase (ec 3.4.24.3) when cultured aerobically in buffer containing 5% peptone. the bacterium is non-pathogenic and tests on rabbits indicated that the culture medium was atoxic. the collagenase, which hydrolyzed insoluble and soluble native collagen, was purified by (nh4)2 so4 precipitation, starch block electrophoresis, and gel filtration. it was shown to be serologically distinct from clostridium histolyticum collagenase and to have molecular weight and se ... | 1975 | 165833 |
| the participation of cytochromes in the reduction of n20 to n2 by a denitryfying bacterium. | the oxidation of cytochromes during the reduction of n2o to n2 by a denitrifying bacterium was studied spectrophotometrically. the reduced b- and c-type cytochromes are partially oxidized when n2o is added to intact cells reduced with lactate under anaerobic conditions. the oxidation of cytochromes is inhibited non-competitively by azide, cyanide, 2,4-dinitrophenol and cuso4, which inhibit the reduction of n2o to n2. in the presence of each inhibitor at a high concentration, at which the reducti ... | 1975 | 168184 |
| the mode of regulation of bacterial citrate synthase as a taxonomic tool. | | 1975 | 168310 |
| specificity of collagenase from achromobacter iophagus. | | 1975 | 169160 |
| amino acid sequence of cytochrome c' from the purple photosynthetic bacterium rhodospirillum rubrum s1. | the amino acid sequence of cytochrome c' from the purple photosynthetic bacterium rhodospirillum rubrum s1 has been determined and is consistent with homology to cytochrome c' from the nonphotosynthetic bacterium alcaligenes sp. ncib 11015. there is 29% identity in the chosen alignment of these two proteins. r. rubrum cytochrome c' is composed of a single peptide chain of 126 amino acid residues with a single heme covalently bound near the cooh terminus. there is no sequence similarity to mitoc ... | 1975 | 172499 |
| inhibition of collagenase from achromobacter iophagus by synthetic peptides. | | 1975 | 173543 |
| chemical characterization and study of the autodigestion of pure collagenase from achromobacter iophagus. | only one collagenase (ec 3.4.24.3) is produced by the non-pathogenic achromobacter iophagus strain. the chromatography of the crude enzyme on de-32 cellulose followed by gel filtration on sephadex g-100 in the presence of 1 m sodium chloride led to the isolation of a homogeneous enzyme. its specific activity (1.642 mukat/mg) represents the highest value ever obtained for a bacterial collagenase. the amino acid composition of a. iophagus collagenase differs from that of clostridium histolyticum m ... | 1976 | 177067 |
| [structure and function of cytochrome c' (author's transl)]. | | 1976 | 178808 |
| the use of bacterial luciferase and a liquid scintillation spectrometer to assay the enzymatic synthesis of nad. | | 1976 | 179444 |
| [denitrifying bacteria of genus alcaligenes isolated from soil]. | the organism isolated is a small non-sporulating gram-negative rod, motile by means of peritrichous flagellae. it is an oxidase-positive chemo-organotroph utilizing o2,no-3,no-2 and n2o as resiratory substrates. primary alcohols as well as numerous organic and amino acids are utilized as carbon and energy sources. carbohydrates are not assimilated. poly-beta-hydroxybutyrate is accumulated intracellularly. the bacterium is assigned to the genus alcaligenes and its phenotype characteristics are co ... | 1975 | 179721 |
| achromobacter cycloclastes nitrite reductase. the function of copper, amino acid composition, and esr spectra. | 1. dialysis against cyanide at ph 7 of achromobacter cycloclastes nitrite reductase [ec 1.7.99.3] of a dissimilatory type led to the removal of about 50% of the copper from the enzyme molecule, with a concomitant decrease of the enzymatic activities. it was inferred that enzyme-bound copper atoms play an essential role in the catalytic activities of the enzyme. 2. the amino acid composition of the enzyme was determined after acid hydrolysis. 3. esr spectra of the frozen solution and lyophilized ... | 1975 | 179983 |
| respiratory components and oxidase activities in alcaligenes eutrophus. | 1. cells of the hydrogen bacterium alcaligenes eutrophus are broken by gentle lysis using lysozyme treatment in hypertonic sucrose followed by osmotic shock. by this method, 93% of the in vivo activity of the h2 oxidase is recovered and the atpase remains particle bound. in contrast, cell disruption in a french pressure cell diminishes the in vivo activity of the h2 oxidase by 50% and solubilizes the bulk of the atpase. 2. the bacterium contains a periplasmic cytochrome c with bands at 418, 521 ... | 1976 | 182246 |
| cleavage of beta-casein by collagenases from achromobacter iophagus and clostridium histolyticum. | | 1976 | 182537 |
| [hydrocarbon metabolism in a marine bacterium]. | the marine bacterium l.16.1 (alcaligenes sp.) grows preferentially on alkanes (c10 to c18) with a very high growth yield (98 per cent); optimal growth depends strictly on the presence of a well-defined nacl concentration (100 mm). our strain is constitutive for the enzymatic systems responsible for the oxidation of alkanes to fatty acids, i.e. nadh-dependent hydroxylase, alcohol and aldehyde dehydrogenases, the latter of which located at the cytoplasmic membrane level. the aerobic oxidation of p ... | 1976 | 184846 |
| the fifth a.j. kluyver memorial lecture delivered before the netherlands society for microbiology on october 9th, 1975, at the delft university of technology, delft. the physiology of hydrogen bacteria. | | 1976 | 185955 |
| purification and properties of soluble hydrogenase from alcaligenes eutrophus h 16. | the soluble hydrogenase (hydrogen: nad+ oxidoreductase, ec 1.12.1.2) from alcaligenes eutrophus h 16 was purified 68-fold with a yield of 20% and a final specific activity (nad reduction) of about 54 mumol h2 oxidized/min per mg protein. the enzyme was shown to be homogenous by polyacrylamide gel electrophoresis. its molecular weight and isoelectric point were determined to be 205 000 and 4.85 respectively. the oxidized hydrogenase, as purified under aerobic conditions, was of high stability but ... | 1976 | 186126 |
| the induction of collagenase and a neutral proteinase by their high molecular weight substrates in achromobacter iophagus. | | 1976 | 187752 |
| comparative effect of methioninyl adenylate on the growth of salmonella typhimurium and pseudomonas aeruginosa. | the bacteriostatic effect of methioninyl adenylate(mamp)--a specific inhibitor of the enzyme methionyl-trna synthetase--was investigated on salmonella typhimurium and pseudomonas aeruginosa. 0.1 mm of this molecule added to the culture, inhibits the growth of s. typhimurium. the inhibition is specifically reversible by 0.1 mm l-methionine. in the same conditions even 1-2 mm mamp has a very slight effect on the growth rate of p. aeruginosa and only during the first two generations. the same obser ... | 1976 | 189717 |
| glycollate production and excretion by alcaligenes eutrophus. | autotrophic cultures of the facultative chemolithotroph alcaligenes eutrophus have been found to excrete glycollate. this excretion was greatly stimulated by the incorporation of up to 20% (v/v) oxygen in the hydrogen used for gassing. the stimulatory effect of oxygen was prevented by the addition of 10% (v/v) co2 to the gassing mixture. glycollate excretion only in the presence of oxygen was increased by the addition of 2-pyridyl-hydroxymethane sulphonic acid (hpms), an inhibitor of glycollate ... | 1976 | 189720 |
| the stereochemical course of the hydrogen transfer to nad, catalyzed by bacterial glucose dehydrogenase and hydrogenase of alcaligenes eutrophus h 16. | | 1977 | 191293 |
| photokinetic assay of pyruvate in the islets of langerhans using bacterial luciferase. | | 1977 | 192101 |
| [hydrogenase activity of the hydrogen-oxidizing bacterium alcaligenes eutrophus]. | | 1977 | 198641 |
| micromorphology of gram-negative hydrogen bacteria. ii. cell envelope, membranes, and cytoplasmic inclusions. | the fine structure of the cell envelope, of membrane systems and of cytoplasmic inclusions of gram-negative aerobic hydrogen bacteria has been studied. the results have been tabulated, and three main groups could be recognized: group 1: alcaligenes eutrophus, a.paradoxus, a.ruhlandii, pseudomonas facilis, p.flava, p.pseudoflava, p.palleronii, and aquaspirillum autotrophicum; group 2: "corynebacterium" autotrophicum and strains ma 2 and sa 35; group 3: paracoccus denitrificans. special structur ... | 1977 | 199126 |
| synthesis of peptide inhibitors of the collagenase from achromobacter iophagus. | | 1977 | 201387 |
| subunit structure of achromobacter collagenase. | the highly active form of collagenase (ec 3.4.24.3) from achromobacter iophagus (specific activity 2 microkat/mg) has a molecular weight of 70,000 and the sedimentation coefficient s20,2 = 4.4 s. it is composed of two subunits of molecular weight 35,000 and s20,w of 2.9 s. the dissociation of the dimer under different conditions resulted in the complete and irreversible loss of enzymic activity. a unique n-terminal sequence thr-ala-ala-asp-leu-glu-ala-leu-val- indicates that the two subunits are ... | 1978 | 202322 |
| metabolism of cyclohexane carboxylic acid by alcaligenes strain w1. | thirty-three microorganisms capable of growth with cyclohexane carboxylate as the sole source of carbon were isolated from mud, water, and soil samples from the aberystwyth area. preliminary screening and whole-cell oxidation studies suggested that, with one exception, all of the strains metabolized the growth substrate by beta-oxidation of the coenzyme a ester. this single distinctive strain, able to oxidize rapidly trans-4-hydroxycyclohexane carboxylate, 4-ketocyclohexane carboxylate, p-hydrox ... | 1978 | 207665 |
| phosphatase activity of aerobic and facultative anaerobic bacteria. | 1115 strains of aerobic and facultatively anaerobic bacteria were tested for phosphatase activity by a conventional plate method and a microtest. the microtest was devised to allow results to be read after 4 h cultivation. phosphatase activity was found in wide range of species and strains. besides staphylococci, where the test for phosphatase is successfully used, it may be applied as one of the valuable tests for the differentiation of the following species: bacillus cereus, b. licheniformis, ... | 1978 | 216188 |
| an oxygen-binding flavohemoprotein from alcaligenes eutrophus. | a procedure is described for the purification of a soluble flavohemoprotein from the hydrogen bacterium alcaligenes eutrophus. the isolated protein exists as a monomer with a molecular weight of approx. 43,000. the molecule contains two prosthetic groups, 1 mol each of noncovalently bound fad and protoheme per monomer. the absorption spectra of the protein in its ferric, ferrous-deoxy and ferrous-carboxy forms are similar to those of hemoglobins, with the exception of the flavin contribution (ab ... | 1979 | 218634 |
| specificity of the collagenolytic enzyme from the fungus entomophthora coronata: comparison with the bacterial collagenase from achromobacter iophagus. | | 1979 | 219780 |
| some newly characterized collagenases from procaryotes and lower eucaryotes. | chemical and enzymatic properties of four collagenases newly isolated from anaerobic clostridium histolyticum, aerobic achromobacter iophagus, and from two lower eucaryotes, the fungus entomophthora coronata and the insect hypoderma lineatum are reviewed. the problems of their biosynthesis and precursors, namely the effect of induction of collagenase and neutral proteinase in achromobacter by their macromolecular substrates are discussed. the two bacterial collagenases are zn-metallo-enzymes; th ... | 1979 | 220520 |
| [taxonomic and classification position of bacteria of the genus bordetella]. | | 1979 | 220821 |
| the amino acid sequence of cytochrome c' from the purple sulphur bacterium chromatium vinosum. | an amino acid sequence is proposed for the cytochrome c' from the photosynthetic purple sulphur bacterium chromatium vinosum strain d. it is single polypeptide chain of 131 residues, with haem-attachment cysteine residues at positions 121 and 124. the results discredit an earlier report [dus, bartsch & kamen (1962) j. biol. chem 237, 3083--3093] of a di-haem peptide sequence from this protein. the sequence belongs to the same class as the published alcaligenes and rhodospirillum rubrum cytochrom ... | 1979 | 220951 |
| differences in the degradation of native collagen by two microbial collagenases. | the early stages of degradation of native collagen by two bacterial collagenases were studied by electron microscopy and by automatic edman degradation. the purified collagenase from clostridium histolyticum was shown to cleave native collagen at several sites, but not progressively from the n-terminus, as had been previously suggested. the homogeneous collagenase from achromobacter iophagus cleaves native collagen preferentially at two sites corresponding to the interbands 33-34 and 41-42. the ... | 1979 | 224860 |
| the iron-sulphur centres of soluble hydrogenase from alcaligenes eutrophus. | the soluble hydrogenase (hydrogen:nad+ oxidoreductase (ec 1.12.1.2) from alcaligenes eutrophus has been purified to homogeneity by an improved procedure, which includes preparative electrophoresis as final step. the specific activity of 57 mumol h2 oxidized/min per mg protein was achieved and the yield of pure enzyme from 200 g cells (wet weight) was about 16 mg/purification. after removal of non-functional iron, analysis of iron and acid-labile sulphur yielded average values of 11.5 and 12.9 at ... | 1979 | 226163 |
| purification and preliminary crystallographic studies on azurin and cytochrome c' from alcaligenes denitrificans and alcaligenes sp. ncib 11015. | | 1979 | 231110 |
| adsorption of vibrio parahaemolyticus onto chitin and copepods. | vibrio parahaemolyticus was observed to adsorb onto chitin particles and copepods. the efficiency of adsorption was found to be dependent on ph and on the concentration of nac1 and other ions found in seawater. highest efficiency was observed in water samples collected from chesapeake bay and lowest in water from the open sea. v. parahaemolyticus was found to adborb onto chitin with the highest efficiency of the several bacterial strains tested. escherichia coli and pseudomonas fluorescens d ... | 1975 | 234715 |
| lipase and esterase formation by psychrophilic and mesophilic acinetobacter species. | acinetobacter o16, a psychrophilic species, produced extracellular lipase (measured by hydrolysis of olive oil, tributyrin, or beta-naphthyl laurate) when grown on a complex medium (peptone plus yeast extract). most lipase was produced during the logarithmic phase of growth. very little cell-bound lipase was formed. these cells also produced an esterase (measured by the hydrolysis of beta-naphthyl acetate). at first, all esterase was cell bound; significant amounts appeared in the external mediu ... | 1975 | 235353 |
| partial purification and characterization of the lipase of a facultatively psychrophilic bacterium (acinetobacter o16). | the extracellular lipase(s) of the psychrophile acinetobacter o16 was studied. when the enzyme was precipitated by (nh4)2so4 and passed through a sephadex g200 column, two peaks of lipase activity appeared. the larger peak, which behaved like a substance of high molecular weight, being eluted in the void volume, was purified 250-fold over the crude enzyme (culture supernatant) by passage through a deae-sephadex column. when the enzyme was applied to a deae-cellulose column it could not be eluted ... | 1975 | 235354 |
| inhibition of nadp-linked malic enzyme by atp in acinetobacter. | | 1975 | 235454 |
| the nature of the attachment of a regularly arranged surface protein to the outer membrane of an acinetobacter sp. | acinetobacter 199a carries on the outer surface of its outer membrane a layer of regularly arranged protein subunits. the isolated surface protein assembles into the same regular array even in the absence of the underlying outer membrane. cl- minus is required for this self-assembly. evidence is presented that the interaction of the surface protein with the outer membrane involves the linking of a carboxyl group in the surface protein to a negatively charged group in the outer membrane protein, ... | 1975 | 237548 |
| the influence of changes in the environment on twitching motility. | by examining medium composition and cultural conditions quantitatively it was possible to define conditions suitable to bring twitching motility about. such conditions include the use of freshly poured, relatively thick and only slightly dried plates of a dilute medium in which the agar concentration is not too high. incubation should take place in a humid atmosphere. in fact, everything points to the humidity as a factor of the utmost importance. using strains of acinetobacter calcoaceticus, it ... | 1975 | 239520 |
| influence of colloidal iron on the respiration of a species of the genus acinetobacter. | washed suspensions of acinetobacter sp. isolated from water caused the precipitation of iron from a suspension of colloidal ferric iron at ph 6.0 and 7.6. iron-encrusted cells of the bacterium formed large aggregates. the amount of iron removed from the colloidal preparation in the form of aggregates was from 21 to 52% at ph 7.6 and 49% an ph 6.0 by the bacterial cells. endogenous respiration rates of the iron-encrusted cells were from 32 to 72% lower than the rates for unencrusted cells. respir ... | 1975 | 239632 |
| aromatic amino acid biosynthesis in alcaligenes eutrophus h16. i. properties and regulation of 3-deoxy-d-arabino heptulosonate 7-phosphate synthase. | properties and regulation of 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase (dahp-synthase), ec4.1.2.15, from alcaligenes eutrophus h16 were investigated. dahp synthase was unstable during manipulations such as dialysis, dilution, ammonium sulfate fractionation, chromatography on deae-cellulose or sephadex g-200. for kinetic measurements sephadex g-25 treated crude extracts were used. the enzyme was not affected by thiol reagents, edta or divalent metal ions. the activation energy, deltah, ... | 1975 | 239657 |
| kinetics and properties of beta-ketothiolase from clostridium pasteurianum. | 1. beta-ketothiolase of clostridium pasteurianum was purified 130-fold by ammonium sulphate fractionation and by column chromatography using deae-sephadex a-50 and hydroxylapatite. subjected to gel electrophoresis beta-ketothiolase revealed two distinct bands; by isoelectric focusing two enzymes with isoelectric points at ph 4.5 and 7.6 were separated. as established by sucrose density gradient centrifugation the molecular weight of both enzymes was found to be 158000. 2. the condensation reacti ... | 1975 | 240336 |
| bacterial respiration-linked proton translocation and its relationship to respiratory-chain composition. | 1. the relationship between chain composition and the efficiency of respiration-linked proton translocation was studied in nine bacterial species of widely differing taxonomic and ecological status. 2. all the bacteria investigated contained respiratory chain dehydrogenases, ubiquinone and/or menaquinone, cytochrome b and cytochrome oxidase aa3 and/or o. in addition, some of these organisms also contained pyridine nucleotide transhydrogenase and/or cytochrome c. 3. leads to h+/o ratios of whole ... | 1975 | 240679 |
| crystalline l-histidine ammonia-lyase of achromobacter liquidum. crystallization and enzymic properties. | crystalline l-histidine ammonia-lyase of achromobacter liquidum was prepared with a 24% recovery of the activity. the specific activity of the pure enzyme (63 mumol of urocanic acid min-1 mg-1) is similar to those so far reported for the enzyme from other sources. the purified enzyme appeared to be homogeneous by analytical disc electrophoresis and isoelectric focusing (pi = 4.95). the molecular weight determined by sephadex g-200 gel filtration is 200000. the optimum ph is 8.2, and the optimum ... | 1975 | 240693 |
| prevalence and survival of microbial contaminants in heated nebulizers. | over a 2-year period, 600 cultures of fluid in heated nebulizers in use by patients in the peter bent brigham hospital were performed. the most commonly isolated microorganisms were pseudomonas and alcaligenes species. pseudomonas aeruginosa was never isolated. three types of heated nebulizers were in use, and their contamination rate was significantly different (45 percent, 21 percent, and 8 percent, respectively; p less than 0.001). in the course of the study, the overall contamination rate de ... | 1978 | 273384 |
| achromobacter l-glutaminase-l-asparaginase: human pharmacology, toxicology, and activity in acute leukemias. | | 1979 | 288507 |
| pyruvate production and excretion by the luminous marine bacteria. | during aerobic growth on glucose, several species of luminous marine bacteria exhibited an imcomplete oxidative catabolism of substrate. pyruvate, one of the products of glucose metabolism, was excreted into the medium during exponential growth and accounted for up to 50% of the substrate carbon metabolized. when glucose was depleted from the medium, the excreted pyruvate was promptly utilized, demonstrating that the cells are capable of pyruvate catabolism. pyruvate excretion is not a general p ... | 1977 | 303077 |
| preliminary observations on the application of the multiple inocula (replicator) method for carbon substrate utilization studies of alcaligenes faecalis. | the use of the replicator technique in evaluating carbon source utilization by 55 cultures of alcaligenes faecalis was examined. six of the 20 substrates tested were utilized by the bacterial cultures. in this study, the reproducibility of the technique was 100%. | 1977 | 323288 |
| differentiation of nonfermentative gram-negative bacilli in the clinical laboratory. | a rapid and simplified system for the differentiation of nonfermentative gram-negative bacilli, encountered frequently in clinical specimens, is presented for use in the clinical laboratory. nonfermentative bacteria can be grouped initially by the motility, oxidase and of glucose reactions. this grouping simplifies the choice of additional tests for further identification. the additional tests included gram stain, acid production from 10% lactose agar, nitrate reduction, arginine dihydrolase act ... | 1977 | 329428 |