| identification of type 4 pili in kingella denitrificans. | kingella denitrificans is an occasionally pathogenic member of the family neisseriaceae and is a member of the normal respiratory flora. electron microscopy, colony morphology types, dna transformation patterns, and immunoblots suggest that k. denitrificans and k. kingae have type 4 pili. this was confirmed by n-terminal amino acid sequencing for k. denitrificans. | 1992 | 1353484 |
| kingella denitrificans as a cause of granulomatous disease in a patient with aids. | | 1992 | 1457643 |
| isolation of kingella denitrificans from amniotic fluid in a woman with chorioamnionitis. a case report. | kingella denitrificans and streptococcus agalactiae were isolated from the amniotic fluid of a woman with chorioamnionitis. she was treated with intravenous ampicillin/sulbactam, with a good response. | 1991 | 1774736 |
| neisseria elongata subsp. nitroreducens subsp. nov., formerly cdc group m-6, a gram-negative bacterium associated with endocarditis. | cdc group m-6 is the vernacular name given to a gram-negative, oxidase-positive, aerobic, nonmotile, rod-shaped bacterium. this organism is biochemically similar to kingella denitrificans and displays a cellular fatty acid profile consistent with cdc groups m-5 and ef-4 and with neisseria elongata. of the 95 m-6 strains referred to the centers for disease control (cdc) for identification, 32 (64%) of the first 50 were from the throat or sputum and only 3 (6%) were from blood; only 5 (11%) of the ... | 1990 | 2279987 |
| [kingella denitrificans endocarditis on an aortic valve prosthesis]. | kingella denitrificans is a gram-negative bacillus which does not grow readily on the usual media. this organism, normally a commensal of the upper airways, may exceptionally be responsible for endocarditis. we report here the sixth case known in the literature. cure was obtained with an intravenous combination of vancomycin and rifampicin. | 1989 | 2530514 |
| cellular fatty acid composition of kingella species, cardiobacterium hominis, and eikenella corrodens. | we determined the cellular fatty acid composition of reference strains and clinical isolates of each of the three kingella species, cardiobacterium hominis, and eikenella corrodens by using capillary gas chromatography. kingella denitrificans and kingella kingae contained myristic (14:0) and palmitic (16:0) acids as major acids, whereas cis-vaccenic (18:1 omega 7c) and palmitic acids were the major acids in kingella indologenes, c. hominis, and e. corrodens. c. hominis differed from the other fo ... | 1988 | 3049663 |
| host range of the conjugative 25.2-megadalton tetracycline resistance plasmid from neisseria gonorrhoeae and related species. | high-level tetracycline resistance in strains of neisseria gonorrhoeae, neisseria meningitidis, kingella denitrificans, and eikenella corrodens has recently been described. the resistance in each species is due to the acquisition of 25.2-megadalton conjugative plasmids that carry the tetracycline resistance determinant tetm. we examined the ability of commensal neisseria species to serve as recipients in conjugation for these new plasmids. most of the recipients (n = 21) tested had detectable co ... | 1988 | 3132092 |
| high-level tetracycline resistance resulting from tetm in strains of neisseria spp., kingella denitrificans, and eikenella corrodens. | similar to neisseria gonorrhoeae, tetracycline-resistant isolates of n. meningitidis, kingella denitrificans, and eikenella corrodens contained 25.2-megadalton plasmids carrying the tetm determinant. in contrast, tetracycline-resistant n. subflava biovar perflava-n. sicca and n. mucosa isolates carried the tetm determinant in the chromosome. | 1988 | 3134850 |
| empyema caused by kingella denitrificans and peptostreptococcus spp. in a patient with bronchogenic carcinoma. | empyema caused by kingella denitrificans and peptostreptococcus spp. was diagnosed in a patient with bronchogenic carcinoma. this appears to be the third report providing evidence of a pathogenic role for k. denitrificans, and the first concerning infection in the pleural space and in a patient with underlying immunosuppressive disease. k. denitrificans should be added to the list of fastidious gram-negative bacteria associated with opportunistic infections in the compromised host. | 1988 | 3229487 |
| campylobacter pyloridis: a new organism to explain an old problem? | | 1987 | 3309916 |
| clinical evaluation of the vitek neisseria-haemophilus identification card. | a clinical evaluation of the vitek neisseria-haemophilus identification (nhi) card (vitek systems, inc., hazelwood, mo.) was performed with 480 clinical isolates and stock strains of neisseria spp., haemophilus spp., and other fastidious microorganisms included in the data base of the system. identifications obtained with the nhi card were compared with those determined by conventional methods. the card identified 83.2% of 244 neisseria spp. and branhamella catarrhalis, 54.9% of 164 haemophilus ... | 1987 | 3539996 |
| septicaemia with probable endocarditis caused by kingella denitrificans. | a 59-year-old man developed septicaemia caused by kingella denitrificans. treatment was with ampicillin and an aminoglycoside. the illness was complicated by a cerebral embolus. an obvious source of infection was not found. | 1987 | 3693925 |
| kingella denitrificans prosthetic endocarditis. | kingella species are rarely reported as a cause of endocarditis. we report two cases of k. denitrificans endocarditis occurring in patients with prosthetic valves. k. denitrificans has not been previously reported to cause prosthetic valve endocarditis. both patients may have developed bacteremia from altered upper respiratory or oral mucosa with subsequent prosthetic valve infection. treatment with penicillin g and streptomycin was successful in the first patient, whereas ampicillin alone resul ... | 1986 | 3953636 |
| differentiation of kingella denitrificans from neisseria gonorrhoeae by growth on a semisolid medium and sensitivity to amylase. | all 239 strains of neisseria gonorrhoeae tested for sensitivity to amylase-impregnated disks showed zones of growth inhibition ranging from 15 to 30 mm in diameter. none of 52 strains of kingella denitrificans were inhibited by the same amylase test. in addition, all n. gonorrhoeae strains grew well on a gonococcal base medium supplemented with either isovitalex (bbl microbiology systems) or supplement b (difco laboratories), whereas k. denitrificans grew adequately only on the medium with isovi ... | 1983 | 6187768 |
| infective endocarditis caused by kingella denitrificans. | the clinical and bacteriological findings in a case of infective endocarditis caused by kingella denitrificans are presented. this appears to be only the second report providing clear evidence for a pathogenic role for this species. | 1984 | 6511984 |
| emended description of kingella denitrificans (snell and lapage 1976): correction of the maltose reaction. | snell and lapage proposed that the bacterium designated cdc group tm-1 be named kingella denitrificans. their description of the organism indicated that it produced acid from maltose which was in contrast with an earlier report from our laboratory that maltose was not utilized. in an attempt to explain the different findings in the two laboratories, the type strain and two additional strains of k. denitrificans were tested for acid production from maltose in phenol red broth, the rapid fermentat ... | 1983 | 6643668 |
| infective endocarditis due to kingella denitrificans. | | 1980 | 7396307 |
| comparison of monoclonal antibody methods and a ribosomal ribonucleic acid probe test for neisseria gonorrhoeae culture confirmation. | recently, a chemiluminescent nucleic acid probe test that specifically detects the ribosomal ribonucleic acid of neisseria gonorrhoeae has been released for clinical laboratory use (accuprobe neisseria gonorrhoeae). in this study, three coagglutination tests (gonogen i, meritec gc, and gc omni), the gonogen ii immunofiltration method and the micro trak neisseria gonorrhoeae fluorescent monoclonal antibody test were compared with accuprobe for identification of gonococci. strains tested (n = 376) ... | 1993 | 7685283 |
| endocarditis caused by kingella denitrificans. | we report the case of a 48-year-old man with native valve endocarditis caused by kingella denitrificans. he was successfully treated with a combination of beta-lactam antibiotics and aminoglycosides, after which he underwent valve replacement surgery. this case represents the first report in the literature of elective native valve replacement. previously reported cases are discussed together with management options, including suggestions for the treatment of patients with beta-lactam allergy and ... | 1993 | 8308323 |
| phylogeny of species in the family neisseriaceae isolated from human dental plaque and description of kingella oralis sp. nov [corrected]. | fourteen human periodontal isolates recovered from a purported eikenella corrodens-selective medium containing 1 microgram of clindamycin per ml displayed biochemical traits which differed from those described for e. corrodens. these organisms were gram-negative rods which corroded agar. the isolates were oxidase positive and urease, indole, and esculin negative. they differed from e. corrodens in catalase, nitrate reduction, lysine decarboxylase, and ornithine decarboxylase activities. one isol ... | 1993 | 8347509 |
| [vaginitis caused by kingella denitrificans in a 3-year-old female patient]. | | 1993 | 8399481 |
| detection of two groups of 25.2 mda tet m plasmids by polymerase chain reaction of the downstream region. | forty-four neisseria gonorrhoeae, 12 n. meningitidis, four kingella denitrificans and one eikenella corrodens carrying 25.2 mda tet m plasmids were analysed using polymerase chain reaction (pcr) to the downstream region of the incomplete tet m transposon. from each isolate, one of two different pcr fragments of approximately 700 or 1600 bp were obtained. the two different sized pcr fragments had > or = 90% dna sequence identity with ureaplasma urealyticum tet m downstream sequences. the differen ... | 1995 | 8569773 |
| identification of four complete type 4 pilin genes in a single kingella denitrificans genome. | we have cloned and sequenced four complete type 4 pilin genes from the type strain (atcc 33394) of kingella denitrificans. two of these pilin genes, kdpb and kdpd, are in tandem, oriented in the same direction, and encode pilins of only 50% amino acid identity. the kdpa and kdpc loci are separately located from the kdpb-kdpd locus and from each other. at the dna level kdpa and kdpc are nearly identical to kdpb and encode pilin proteins that are identical to kdpb. bands of multiple hybridization ... | 1996 | 8945537 |
| type-4 pili of kingella denitrificans. | kingella denitrificans possess type-4 pili, and the type strain, atcc 33394, contains at least four complete copies of type-4 pilin-encoding genes. previously reported hybridization patterns of k. denitrificans chromosomal dna seen using a neisseria gonorrhoeae pilin gene region probe, had been interpreted as representing possible partial, silent gene loci. this now appears to be due to cross-reaction to multiple copies of 18-bp inverted repeat structures. data are presented on a variety of colo ... | 1997 | 9224888 |
| [prosthetic endocarditis caused by kingella denitrificans in a patient with diabetes mellitus]. | infective endocarditis caused by kingella denitrificans occurs rarely. a review of the literature reveals only 6 cases of endocarditis caused by the bacillus. k. denitrificans is normally a commensal of the upper respiratory airways, may exceptionally be responsible for endocarditis. a case of possible prosthetic endocarditis caused by k. denitrificans is presented. a 78-year-old male with type ii diabetes was admitted to the hospital complaining of fever, a sore throat and arthralgia. he underw ... | 1997 | 9283146 |
| erythromycin-resistant neisseria gonorrhoeae and oral commensal neisseria spp. carry known rrna methylase genes. | two neisseria gonorrhoeae isolates from seattle and two isolates from uruguay were resistant to erythromycin (mic, 4 to 16 microg/ml) and had reduced susceptibility to azithromycin (mic, 1 to 4 microg/ml) due to the presence of the self-mobile rrna methylase gene(s) ermf or ermb and ermf. the two seattle isolates and one isolate from uruguay were multiresistant, carrying either the 25.2-mda tetm-containing plasmid (seattle) or a beta-lactamase plasmid (uruguay). sixteen commensal neisseria isola ... | 1999 | 10348754 |
| identification of the conjugative mef gene in clinical acinetobacter junii and neisseria gonorrhoeae isolates. | the mef gene, originally described for gram-positive organisms and coding for an efflux pump, has been identified in clinical isolates of acinetobacter junii and neisseria gonorrhoeae. these strains could transfer the mef gene at frequencies ranging from 10(-6) to 10(-9) into one or more of the following recipients: gram-negative moraxella catarrhalis, neisseria perflava/sicca and neisseria mucosa and gram-positive enterococcus faecalis. three streptococcus pneumoniae strains could transfer the ... | 2000 | 10952602 |
| endocarditis due to rare and fastidious bacteria. | the etiologic diagnosis of infective endocarditis is easily made in the presence of continuous bacteremia with gram-positive cocci. however, the blood culture may contain a bacterium rarely associated with endocarditis, such as lactobacillus spp., klebsiella spp., or nontoxigenic corynebacterium, salmonella, gemella, campylobacter, aeromonas, yersinia, nocardia, pasteurella, listeria, or erysipelothrix spp., that requires further investigation to establish the relationship with endocarditis, or ... | 2001 | 11148009 |
| diagnostics of neisseriaceae and moraxellaceae by ribosomal dna sequencing: ribosomal differentiation of medical microorganisms. | fast and reliable identification of microbial isolates is a fundamental goal of clinical microbiology. however, in the case of some fastidious gram-negative bacterial species, classical phenotype identification based on either metabolic, enzymatic, or serological methods is difficult, time-consuming, and/or inadequate. 16s or 23s ribosomal dna (rdna) bacterial sequencing will most often result in accurate speciation of isolates. therefore, the objective of this study was to find a hypervariable ... | 2001 | 11230407 |
| pseudomonas stutzeri has two closely related pila genes (type iv pilus structural protein) with opposite influences on natural genetic transformation. | pseudomonas stutzeri has type iv pili for which the pila gene (here termed pilai) provides the structural protein and which are required for dna uptake and natural genetic transformation. downstream of pilai we identified a gene, termed pilaii, coding for a deduced protein with a size similar to that of pilai with 55% amino acid sequence identity and with a typical leader peptide including a leader peptidase cleavage site. fusions to lacz revealed that pilaii is expressed only about 10% compared ... | 2001 | 11244078 |
| tetracycline antibiotics: mode of action, applications, molecular biology, and epidemiology of bacterial resistance. | tetracyclines were discovered in the 1940s and exhibited activity against a wide range of microorganisms including gram-positive and gram-negative bacteria, chlamydiae, mycoplasmas, rickettsiae, and protozoan parasites. they are inexpensive antibiotics, which have been used extensively in the prophlylaxis and therapy of human and animal infections and also at subtherapeutic levels in animal feed as growth promoters. the first tetracycline-resistant bacterium, shigella dysenteriae, was isolated i ... | 2001 | 11381101 |
| comparative evaluation of the bd phoenix and vitek 2 automated instruments for identification of isolates of the burkholderia cepacia complex. | we evaluated two new automated identification systems, the bd phoenix (becton dickinson) and the vitek 2 (biomérieux), for identification of isolates of the burkholderia cepacia complex (bcc). the test sample included 42 isolates of the highly virulent and epidemic genomovar iii, 45 isolates of b. multivorans, and 47 isolates of other members of the bcc. rates of correct identification by the bd phoenix and vitek 2 were similar when all bcc isolates were considered (50 and 53%, respectively) but ... | 2002 | 11980954 |
| distribution of mef(a) in gram-positive bacteria from healthy portuguese children. | we screened 615 gram-positive isolates from 150 healthy children for the presence of the erm(a), erm(b), erm(c), erm(f), and mef(a) genes. the mef(a) genes were found in 20 (9%) of the macrolide-resistant isolates, including enterococcus spp., staphylococcus spp., and streptococcus spp. sixteen of the 19 gram-positive isolates tested carried the other seven open reading frames (orfs) described in tn1207.1, a genetic element carrying mef(a) recently described in streptococcus pneumoniae. the thre ... | 2002 | 12121926 |
| usefulness of the microseq 500 16s ribosomal dna-based bacterial identification system for identification of clinically significant bacterial isolates with ambiguous biochemical profiles. | due to the inadequate automation in the amplification and sequencing procedures, the use of 16s rrna gene sequence-based methods in clinical microbiology laboratories is largely limited to identification of strains that are difficult to identify by phenotypic methods. in this study, using conventional full-sequence 16s rrna gene sequencing as the "gold standard," we evaluated the usefulness of the microseq 500 16s ribosomal dna (rdna)-based bacterial identification system, which involves amplifi ... | 2003 | 12734240 |
| bacterial community dynamics during production of registered designation of origin salers cheese as evaluated by 16s rrna gene single-strand conformation polymorphism analysis. | microbial dynamics during processing and ripening of traditional cheeses such as registered designation of origin salers cheese, an artisanal cheese produced in france, play an important role in the elaboration of sensory qualities. the aim of the present study was to obtain a picture of the dynamics of the microbial ecosystem of rdo salers cheese by using culture-independent methods. this included dna extraction, pcr, and single-strand conformation polymorphism (sscp) analysis. bacterial and hi ... | 2003 | 12839752 |
| the mef(a) gene predominates among seven macrolide resistance genes identified in gram-negative strains representing 13 genera, isolated from healthy portuguese children. | of the 176 randomly selected, commensal, gram-negative bacteria isolated from healthy children with low exposure to antibiotics, 138 (78%) carried one or more of the seven macrolide resistance genes tested in this study. these isolates included 79 (91%) isolates from the oral cavity and 59 (66%) isolates from urine samples. the mef(a) gene, coding for an efflux protein, was found in 73 isolates (41%) and was the most frequently carried gene. the mef(a) gene could be transferred from the donors i ... | 2004 | 15328110 |
| identification of candidate periodontal pathogens and beneficial species by quantitative 16s clonal analysis. | most studies of the bacterial etiology of periodontitis have used either culture-based or targeted dna approaches, and so it is likely that pathogens remain undiscovered. the purpose of this study was to use culture-independent, quantitative analysis of biofilms associated with chronic periodontitis and periodontal health to identify pathogens and beneficial species. samples from subjects with periodontitis and controls were analyzed using ribosomal 16s cloning and sequencing. several genera, ma ... | 2005 | 16081935 |
| microbial risk indicators of early childhood caries. | the aim of this study was to use molecular identification methods, such as 16s rna gene sequence and reverse-capture checkerboard hybridization, for identification of the bacteria associated with dental caries and with dental health in a subset of 204 twins aged 1.5 to 7 years old. a total of 448 plaque samples (118 collected from caries-free subjects and 330 from caries-active subjects) were used for analysis. we compared the bacteria found in biofilms of children exhibiting severe dental carie ... | 2005 | 16272513 |
| the clinical diagnosis of microbial keratitis. | to evaluate the ability of ophthalmologists to predict the laboratory results of presumed microbial keratitis and to explore which findings may influence diagnostic prognostication. | 2007 | 17408586 |
| bacterial peritonitis caused by kingella kingae. | kingella kingae is a commensal of the upper respiratory tract that occasionally causes skeletal infections in children and endocarditis in children and adults. we report a case of a 55-year-old man with liver disease and tense ascites who performed a paracentesis on himself and developed k. kingae peritonitis and bacteremia. | 2007 | 17634309 |
| the laboratory diagnosis of neisseria gonorrhoeae. | the present article describes the laboratory diagnosis of neisseria gonorrhoeae by culturing of the organism from different types of clinical specimens followed by confirmatory tests. the success of culture methods requires good quality collection and transport of clinical specimens. the present guide describes the media requirements and cultural conditions for n gonorrhoeae growth and the characteristics for a presumptive identification of n gonorrhoeae. confirmatory tests include biochemical t ... | 2005 | 18159523 |
| multicenter evaluation of the new vitek 2 neisseria-haemophilus identification card. | the new neisseria-haemophilus identification (nh) card for vitek 2 was compared with 16s rrna gene sequencing (16s) as the reference method for accurate identification of neisseria spp., haemophilus spp., and other fastidious gram-negative bacteria. testing was performed on the vitek 2 xl system with modified software at three clinical trial laboratories. reproducibility was determined with nine atcc quality control strains tested 20 times over a minimum of 10 days at all three sites. a challeng ... | 2008 | 18579712 |
| molecular detection of multiple emerging pathogens in sputa from cystic fibrosis patients. | there is strong evidence that culture-based methods detect only a small proportion of bacteria present in the respiratory tracts of cystic fibrosis (cf) patients. | 2008 | 18682840 |
| kingella kingae expresses type iv pili that mediate adherence to respiratory epithelial and synovial cells. | kingella kingae is a gram-negative bacterium that colonizes the respiratory tract and is a common cause of septic arthritis and osteomyelitis. despite the increasing frequency of k. kingae disease, little is known about the mechanism by which this organism adheres to respiratory epithelium and seeds joints and bones. previous work showed that k. kingae expresses long surface fibers that vary in surface density. in the current study, we found that these fibers are type iv pili and are necessary f ... | 2008 | 18757541 |
| new real-time pcr-based method for kingella kingae dna detection: application to samples collected from 89 children with acute arthritis. | inoculation of blood culture vials with joint fluid samples has revealed the important pathogenic role of kingella kingae in pediatric arthritis. however, recent studies based on broad-range 16s ribosomal dna pcr and real-time pcr without a probe suggest that conventional methods remain suboptimal. we developed a new real-time pcr method with a probe that is highly specific for k. kingae and applied it to joint fluid samples collected from 89 children with suspected arthritis admitted to our ins ... | 2009 | 19369442 |
| rapid differentiation of francisella species and subspecies by fluorescent in situ hybridization targeting the 23s rrna. | francisella (f.) tularensis is the causative agent of tularemia. due to its low infectious dose, ease of dissemination and high case fatality rate, f. tularensis was the subject in diverse biological weapons programs and is among the top six agents with high potential if misused in bioterrorism. microbiological diagnosis is cumbersome and time-consuming. methods for the direct detection of the pathogen (immunofluorescence, pcr) have been developed but are restricted to reference laboratories. | 2010 | 20205957 |
| relationship between oral malodor and the global composition of indigenous bacterial populations in saliva. | oral malodor develops mostly from the metabolic activities of indigenous bacterial populations within the oral cavity, but whether healthy or oral malodor-related patterns of the global bacterial composition exist remains unclear. in this study, the bacterial compositions in the saliva of 240 subjects complaining of oral malodor were divided into groups based on terminal-restriction fragment length polymorphism (t-rflp) profiles using hierarchical cluster analysis, and the patterns of the microb ... | 2010 | 20228112 |
| the analysis of oral microbial communities of wild-type and toll-like receptor 2-deficient mice using a 454 gs flx titanium pyrosequencer. | although mice have long served as an animal model for periodontitis, information on the composition of their indigenous oral microbiota is limited. the aim of the current study was to characterize mouse oral bacterial flora by applying extensive parallel pyrosequencing using the latest model pyrosequencer, a roche/454 genome sequencer flx titanium. in addition, the effect of toll-like receptor (tlr) 2 deficiency on oral microbiota was evaluated. | 2010 | 20370919 |
| bacterial 16s sequence analysis of severe caries in young permanent teeth. | previous studies have confirmed the association of the acid producers streptococcus mutans and lactobacillus spp. with childhood caries, but they also suggested these microorganisms are not sufficient to explain all cases of caries. in addition, health-associated bacterial community profiles are not well understood, including the importance of base production and acid catabolism in ph homeostasis. the bacterial community composition in health and in severe caries of the young permanent dentition ... | 2010 | 20826648 |
| isolation of kingella denitrificans from a corneal ulcer. | to report a case of a corneal ulcer caused by an unusual pathogen. | 2011 | 21099406 |
| the rtxa toxin gene of kingella kingae: a pertinent target for molecular diagnosis of osteoarticular infections. | kingella kingae is an emerging osteoarticular pathogen in young children. its isolation by traditional culture methods remains difficult, underscoring the need to implement other diagnostic methods for its detection and identification, such as nucleic acid amplification tests. although the genome of this bacterium has not yet been sequenced, a toxin named rtx has been identified. the goal of this study was to develop sensitive, specific, and rapid molecular methods based on the rtxa toxin gene s ... | 2011 | 21248099 |
| the burden of invasive early-onset neonatal sepsis in the united states, 2005-2008. | background:: sepsis in the first 3 days of life is a leading cause of morbidity and mortality among infants. group b streptococcus (gbs), historically the primary cause of early-onset sepsis (eos), has declined through widespread use of intrapartum chemoprophylaxis. we estimated the national burden of invasive eos cases and deaths in the era of gbs prevention. methods:: population-based surveillance for invasive eos was conducted in 4 of the centers for disease control and prevention's active ba ... | 2011 | 21654548 |
| retropharyngeal abscess from an unusual organism-denitrificans-in a patient on low-dose methotrexate. | retropharyngeal abscess can be a life-threatening emergency with potential for airway compromise. we report a case of retropharyngeal abscess caused by kingella denitrificans in a 66-year-old caucasian man taking low-dose methotrexate for psoriatic arthritis. k denitrificans has not been previously reported as a cause of retropharyngeal abscess. k denitrificans, along with other kingella species, is often found to be part of the normal upper respiratory tract flora. potentially life-threatening ... | 2011 | 21792784 |
| integrated analysis of three bacterial conjunctivitis trials of besifloxacin ophthalmic suspension, 0.6%: etiology of bacterial conjunctivitis and antibacterial susceptibility profile. | the purpose of this paper is to report on the bacterial species isolated from patients with bacterial conjunctivitis participating in three clinical trials of besifloxacin ophthalmic suspension, 0.6%, and their in vitro antibacterial susceptibility profiles. | 2011 | 22034556 |
| ehrlichia chaffeensis tandem repeat proteins and ank200 are type 1 secretion system substrates related to the repeats-in-toxin exoprotein family. | ehrlichia chaffeensis has type 1 and 4 secretion systems (t1ss and t4ss), but the substrates have not been identified. potential substrates include secreted tandem repeat protein (trp) 47, trp120, and trp32, and the ankyrin repeat protein, ank200, that are involved in molecular host-pathogen interactions including dna binding and a network of protein-protein interactions with host targets associated with signaling, transcriptional regulation, vesicle trafficking, and apoptosis. in this study we ... | 2011 | 22919588 |
| molecular mapping to species level of the tonsillar crypt microbiota associated with health and recurrent tonsillitis. | the human palatine tonsils, which belong to the central antigen handling sites of the mucosal immune system, are frequently affected by acute and recurrent infections. this study compared the microbiota of the tonsillar crypts in children and adults affected by recurrent tonsillitis with that of healthy adults and children with tonsillar hyperplasia. an in-depth 16s rrna gene based pyrosequencing approach combined with a novel strategy that included phylogenetic analysis and detection of species ... | 2013 | 23437130 |
| the influence of smoking on the peri-implant microbiome. | smokers are at high risk for 2 bacterially driven oral diseases: peri-implant mucositis and peri-implantitis. therefore, the purpose of this investigation was to use a deep-sequencing approach to identify the effect of smoking on the peri-implant microbiome in states of health and disease. peri-implant biofilm samples were collected from 80 partially edentulous subjects with peri-implant health, peri-implant mucositis, and peri-implantitis. bacterial dna was isolated and 16s ribsomal rna gene li ... | 2015 | 26124222 |
| subgingival microbiome in patients with healthy and ailing dental implants. | dental implants are commonly used to replace missing teeth. however, the dysbiotic polymicrobial communities of peri-implant sites are responsible for peri-implant diseases, such as peri-implant mucositis and peri-implantitis. in this study, we analyzed the microbial characteristics of oral plaque from peri-implant pockets or sulci of healthy implants (n = 10), peri-implant mucositis (n = 8) and peri-implantitis (n = 6) sites using pyrosequencing of the 16s rrna gene. an increase in microbial di ... | 2015 | 26077225 |
| subgingival microbiota in health compared to periodontitis and the influence of smoking. | the etiology of periodontitis has traditionally been associated to a consortium of three bacterial species-the so-called "red-complex" of periodontal disease-which has been the target for most diagnostic and therapeutic strategies. however, other species have also been found to correlate with disease severity. in addition, the influence of smoking on periodontal microbiota is poorly understood. in the current manuscript, the composition of the subgingival microbiota in healthy individuals vs. pa ... | 2015 | 25814980 |
| functional genes to assess nitrogen cycling and aromatic hydrocarbon degradation: primers and processing matter. | targeting sequencing to genes involved in key environmental processes, i.e., ecofunctional genes, provides an opportunity to sample nature's gene guilds to greater depth and help link community structure to process-level outcomes. vastly different approaches have been implemented for sequence processing and, ultimately, for taxonomic placement of these gene reads. the overall quality of next generation sequence analysis of functional genes is dependent on multiple steps and assumptions of unknow ... | 2013 | 24062736 |
| accurate identification of fastidious gram-negative rods: integration of both conventional phenotypic methods and 16s rrna gene analysis. | accurate identification of fastidious gram-negative rods (gnr) by conventional phenotypic characteristics is a challenge for diagnostic microbiology. the aim of this study was to evaluate the use of molecular methods, e.g., 16s rrna gene sequence analysis for identification of fastidious gnr in the clinical microbiology laboratory. | 2013 | 23855986 |
| dysbiotic infection in the stomach. | microbiota in human alimentary tract plays important roles for homeostatic maintenance of the body. compositional difference of gut microbiota is tightly associated with susceptibility of many diseases, including inflammatory diseases, obesity, diabetes mellitus, cancer, and atherosclerosis. "dysbiosis" refers to a state of imbalance among the colonies of microorganisms within the body, which brings abnormal increase of specific minor components and decrease in the normally dominant species. sin ... | 2015 | 26523109 |
| a reproducible oral microcosm biofilm model for testing dental materials. | most studies of biofilm effects on dental materials use single-species biofilms, or consortia. microcosm biofilms grown directly from saliva or plaque are much more diverse, but difficult to characterize. we used the human oral microbial identification microarray (homim) to validate a reproducible oral microcosm model. | 2012 | 22925110 |
| the dental plaque microbiome in health and disease. | dental decay is one of the most prevalent chronic diseases worldwide. a variety of factors, including microbial, genetic, immunological, behavioral and environmental, interact to contribute to dental caries onset and development. previous studies focused on the microbial basis for dental caries have identified species associated with both dental health and disease. the purpose of the current study was to improve our knowledge of the microbial species involved in dental caries and health by perfo ... | 2013 | 23520516 |
| comparison of oral microbial profiles between children with severe early childhood caries and caries-free children using the human oral microbe identification microarray. | early childhood caries (ecc) has become a prevalent public health problem among chinese preschool children. the bacterial microflora is considered to be an important factor in the formation and progress of dental caries. however, high-throughput and large-scale studies of the primary dentition are lacking. the present study aimed to compare oral microbial profiles between children with severe ecc (secc) and caries-free children. | 2015 | 25821962 |
| dialects of the dna uptake sequence in neisseriaceae. | in all sexual organisms, adaptations exist that secure the safe reassortment of homologous alleles and prevent the intrusion of potentially hazardous alien dna. some bacteria engage in a simple form of sex known as transformation. in the human pathogen neisseria meningitidis and in related bacterial species, transformation by exogenous dna is regulated by the presence of a specific dna uptake sequence (dus), which is present in thousands of copies in the respective genomes. dus affects transform ... | 2013 | 23637627 |
| heme synthesis and acquisition in bacterial pathogens. | bacterial pathogens require the iron-containing cofactor heme to cause disease. heme is essential to the function of hemoproteins, which are involved in energy generation by the electron transport chain, detoxification of host immune effectors, and other processes. during infection, bacterial pathogens must synthesize heme or acquire heme from the host; however, host heme is sequestered in high-affinity hemoproteins. pathogens have evolved elaborate strategies to acquire heme from host sources, ... | 2016 | 27019298 |
| microbial communities from methane hydrate-bearing deep marine sediments in a forearc basin. | microbial communities in cores obtained from methane hydrate-bearing deep marine sediments (down to more than 300 m below the seafloor) in the forearc basin of the nankai trough near japan were characterized with cultivation-dependent and -independent techniques. acridine orange direct count data indicated that cell numbers generally decreased with sediment depth. lipid biomarker analyses indicated the presence of viable biomass at concentrations greater than previously reported for terrestrial ... | 2002 | 12147470 |
| exploring preterm birth as a polymicrobial disease: an overview of the uterine microbiome. | infection is a leading cause of preterm birth (ptb). a focus of many studies over the past decade has been to characterize microorganisms present in the uterine cavity and document any association with negative pregnancy outcome. a range of techniques have been used to achieve this, including microbiological culture and targeted polymerase chain reaction assays, and more recently, microbiome-level analyses involving either conserved, phylogenetically informative genes such as the bacterial 16s r ... | 2014 | 25505898 |
| origin and evolution of antibiotic resistance: the common mechanisms of emergence and spread in water bodies. | the environment, and especially freshwater, constitutes a reactor where the evolution and the rise of new resistances occur. in water bodies such as waste water effluents, lakes, and rivers or streams, bacteria from different sources, e.g., urban, industrial, and agricultural waste, probably selected by intensive antibiotic usage, are collected and mixed with environmental species. this may cause two effects on the development of antibiotic resistances: first, the contamination of water by antib ... | 2012 | 22303296 |
| early-onset neonatal sepsis. | early-onset sepsis remains a common and serious problem for neonates, especially preterm infants. group b streptococcus (gbs) is the most common etiologic agent, while escherichia coli is the most common cause of mortality. current efforts toward maternal intrapartum antimicrobial prophylaxis have significantly reduced the rates of gbs disease but have been associated with increased rates of gram-negative infections, especially among very-low-birth-weight infants. the diagnosis of neonatal sepsi ... | 2014 | 24396135 |
| evaluation of the bruker maldi biotyper for identification of fastidious gram-negative rods. | matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) has entered clinical laboratories, facilitating identification of bacteria. here, we evaluated the maldi biotyper (bruker daltonics) for the identification of fastidious gram-negative rods (gnr). three sample preparation methods, direct colony transfer, direct transfer plus on-target formic acid preparation, and ethanol-formic acid extraction, were analyzed for 151 clinical isolates. direct colony transfe ... | 2016 | 26659214 |
| analysis of mixed sequencing chromatograms and its application in direct 16s rrna gene sequencing of polymicrobial samples. | investigation of clinical samples by direct 16s rrna gene sequencing provides the possibility to detect nonviable bacteria and bacteria with special growth requirements. this approach has been particularly valuable for the diagnosis of patients who have received antibiotics prior to sample collection. in specimens containing more than one bacterium, direct sequencing gives mixed chromatograms that complicate further interpretation. we designed an algorithm able to analyze these ambiguous chromat ... | 2008 | 18768654 |
| genome sequence of kingella kingae septic arthritis isolate pykk081. | kingella kingae is a human oral bacterium that can cause infections of the skeletal system in children. the bacterium is also a cardiovascular pathogen causing infective endocarditis in children and adults. we report herein the draft genome sequence of septic arthritis k. kingae strain pykk081. | 2012 | 22582375 |
| analytical evaluation of genexpert ct/ng, the first genetic point-of-care assay for simultaneous detection of neisseria gonorrhoeae and chlamydia trachomatis. | genexpert ct/ng was evaluated with 372 characterized bacterial strains. sensitivity of 10 genome copies/reaction was obtained for both agents. four neisseria mucosa and two neisseria subflava isolates were positive for one of two gonococcal targets; however, the assay flagged all as negative. the assay was analytically highly sensitive and specific. | 2013 | 23554203 |
| recommendations for the laboratory-based detection of chlamydia trachomatis and neisseria gonorrhoeae--2014. | this report updates cdc's 2002 recommendations regarding screening tests to detect chlamydia trachomatis and neisseria gonorrhoeae infections (cdc. screening tests to detect chlamydia trachomatis and neisseria gonorrhoeae infections-2002. mmwr 2002;51[no. rr-15]) and provides new recommendations regarding optimal specimen types, the use of tests to detect rectal and oropharyngeal c. trachomatis and n. gonorrhoeae infections, and circumstances when supplemental testing is indicated. the recommend ... | 2014 | 24622331 |
| evaluation of the new bd max gc real-time pcr assay, analytically and clinically as a supplementary test for the bd probetec gc qx amplified dna assay, for molecular detection of neisseria gonorrhoeae. | the new bd max gc real-time pcr assay showed high clinical and analytical sensitivity and specificity. it can be an effective and accurate supplementary test for the bd probetec gc qx amplified dna assay, which had suboptimal specificity, and might also be used for initial detection of neisseria gonorrhoeae. | 2015 | 26468501 |
| perioperative microbiologic monitoring of sputum on postoperative day one as a predictor of pneumonia after hepatectomy. | the purpose of this study was to retrospectively evaluate microbial examination of sputum on postoperative day one (pod1) and to determine risk factors for postoperative pneumonia (pop) after hepatectomy. | 2015 | 26105551 |
| bacterial aetiological agents of intra-amniotic infections and preterm birth in pregnant women. | infection-related preterm birth is a leading cause of infant mortality and morbidity; knowledge of bacterial populations invading the amniotic cavity and the routes of invasion is required to make progress in the prevention of preterm birth. significant advances have been made in understanding bacterial communities in the vagina, but much less studied are intra-uterine bacterial populations during pregnancy. a systematic review of data published on the intra-uterine microbiome was performed; mol ... | 2013 | 24137568 |
| bacterial flora concurrent with helicobacter pylori in the stomach of patients with upper gastrointestinal diseases. | to investigate the non-helicobacter pylori (h. pylori) bacterial flora concurrent with h. pylori infection. | 2012 | 22468090 |
| structural analysis of haemoglobin binding by hpua from the neisseriaceae family. | the neisseriaceae family of bacteria causes a range of diseases including meningitis, septicaemia, gonorrhoea and endocarditis, and extracts haem from haemoglobin as an important iron source within the iron-limited environment of its human host. herein we report crystal structures of apo- and haemoglobin-bound hpua, an essential component of this haem import system. the interface involves long loops on the bacterial receptor that present hydrophobic side chains for packing against the surface of ... | 2015 | 26671256 |
| performance of tests for identification of neisseria gonorrhoeae. | | 2015 | 26205027 |
| a case of suppurative peritonitis by a commensal oral organism, kingella denitrificans, in an adult peritoneal dialysis patient. | | 2015 | 25700466 |
| functional analysis of the interdependence between dna uptake sequence and its cognate comp receptor during natural transformation in neisseria species. | natural transformation is the widespread biological process by which "competent" bacteria take up free dna, incorporate it into their genomes, and become genetically altered or "transformed". to curb often deleterious transformation by foreign dna, several competent species preferentially take up their own dna that contains specific dus (dna uptake sequence) watermarks. our recent finding that comp is the long sought dus receptor in neisseria species paves the way for the functional analysis of ... | 2013 | 24385921 |
| specific dna recognition mediated by a type iv pilin. | natural transformation is a dominant force in bacterial evolution by promoting horizontal gene transfer. this process may have devastating consequences, such as the spread of antibiotic resistance or the emergence of highly virulent clones. however, uptake and recombination of foreign dna are most often deleterious to competent species. therefore, model naturally transformable gram-negative bacteria, including the human pathogen neisseria meningitidis, have evolved means to preferentially take u ... | 2013 | 23386723 |
| preclinical studies and prospective clinical applications for bacteria-targeted imaging: the future is bright. | bacterial infections are a frequently occurring and major complication in human healthcare, in particular due to the rapid increase of antimicrobial resistance and the emergence of pan-drug-resistant microbes. current anatomical and functional imaging modalities are insufficiently capable of distinguishing sites of bacterial infection from sterile inflammation. therefore, definitive diagnosis of an infection can often only be obtained by tissue biopsy and subsequent culture and, occasionally, a ... | 2016 | 27512688 |
| hacek infective endocarditis: characteristics and outcomes from a large, multi-national cohort. | the hacek organisms (haemophilus species, aggregatibacter species, cardiobacterium hominis, eikenella corrodens, and kingella species) are rare causes of infective endocarditis (ie). the objective of this study is to describe the clinical characteristics and outcomes of patients with hacek endocarditis (he) in a large multi-national cohort. patients hospitalized with definite or possible infective endocarditis by the international collaboration on endocarditis prospective cohort study in 64 hosp ... | 2013 | 23690995 |
| kingella kingae keratitis. | we present the first two cases of kingella kingae keratitis in adults. this species is a rare isolate from ophthalmic samples for which final identification was obtained with matrix-assisted laser desorption ionization-time of flight mass spectrometry. one of the patients recovered uneventfully with topical therapy. results from the second patient cannot be confirmed as he was lost to follow-up. | 2013 | 23447636 |
| peritoneal dialysis-related peritonitis due to kingella denitrificans: the first case report. | | 2014 | 25520495 |
| genome analysis of kingella kingae strain kwg1 reveals how a β-lactamase gene inserted in the chromosome of this species. | we describe the genome of a penicillinase-producing kingella kingae strain (kwg1), the first to be isolated in continental europe, whose bla(tem-1) gene was, for the first time in this species, found to be chromosomally inserted. the bla(tem) gene is located in an integrative and conjugative element (ice) inserted in met-trna and comprising genes that encode resistance to sulfonamides, streptomycin, and tetracycline. this ice is homologous to resistance-conferring plasmids of k. kingae and other ... | 2015 | 26574009 |
| genome analysis of kingella kingae strain kwg1 reveals how a β-lactamase gene inserted in the chromosome of this species. | we describe the genome of a penicillinase-producing kingella kingae strain (kwg1), the first to be isolated in continental europe, whose bla(tem-1) gene was, for the first time in this species, found to be chromosomally inserted. the bla(tem) gene is located in an integrative and conjugative element (ice) inserted in met-trna and comprising genes that encode resistance to sulfonamides, streptomycin, and tetracycline. this ice is homologous to resistance-conferring plasmids of k. kingae and other ... | 2015 | 26574009 |
| positive predictive values of the international classification of diseases, 10th revision diagnoses of gram-negative septicemia/sepsis and urosepsis for presence of gram-negative bacteremia. | health care databases are a valuable resource for infectious disease epidemiology if diagnoses are accurately coded. we examined the ability of diagnostic coding to accurately identify gram-negative bacteremia. | 2015 | 25709502 |
| mycoplasma edwardii peritonitis in a patient on maintenance peritoneal dialysis. | mycoplasma edwardii (m. edwardii) is an anthropozoonotic microorganism found in the upper respiratory and urogenital tracts of dogs. m. edwardii was one of the microbes isolated from peritoneal fluid of a 10-year-old child diagnosed with polymicrobial peritonitis following a puncture of dialysis tubing by a pet dog. other unique pathogens representative of canine oral microflora isolated from this patient on peritoneal dialysis were kingella denitrificans, actinomycetes species and capnocytophag ... | 2015 | 24040782 |
| low prevalence of kingella kingae carriage in children aged 6-48 months in sydney, australia. | a prospective observational study was conducted to estimate the prevalence of oropharyngeal carriage of kingella kingae in healthy australian pre-school children. | 2017 | 27669685 |
| colonization of an acid resistant kingella denitrificans in the stomach may contribute to gastric dysbiosis by helicobacter pylori. | in the stomach of a gastric ulcer patient who had been administered an anti-acid, a gram-negative and urease-negative bacillus similar in size to helicobacter pylori was infected together with h. pylori. according to biochemical test and 16s rrna gene analysis, the urease-negative bacterium was identified as kingella denitrificans, a human nasopharyngeal commensal. in contrast to the standard strain of k. denitrificans, the isolate showed catalase activity, did not produce acid from glucose, and ... | 2014 | 24462438 |