| identification of some uncommon monounsaturated fatty acids of bacteria. | location of the double-bond position of monounsaturated fatty acids of various bacteria was accomplished with combined gas chromatography-mass spectrometry analysis of dimethyl disulfide (dmds) derivatives. the monoenoic fatty acids from whole cells were converted to methyl esters and then to dmds adducts and analyzed by capillary gas chromatography-mass spectrometry. the mass spectra of dmds adducts gave an easily recognizable molecular ion and two major diagnostic ions attributable to fragment ... | 1992 | 1401029 |
| isolation and characterization by conventional methods and genetic transformation of psychrobacter and acinetobacter from fresh and spoiled meat, milk and cheese. | of 126 samples of fresh and spoiled meat and dairy products, 40% were positive for the presence of moraxella-like bacteria and 64% of acinetobacter; 279 and 466 strains, respectively, were isolated and a part of these were tested by biochemical methods and dna transformation assays. in some cases, the moraxellaceae in the samples examined reached considerable quantitative levels, but their percentage in the microflora was generally low. moraxella-like bacteria were predominant in fresh meat, aci ... | 1992 | 1622760 |
| numerical taxonomy of gram-negative, nonmotile, nonfermentative bacteria isolated during chilled storage of lamb carcasses. | a numerical taxonomic study using 75 characters was performed with 132 strains of gram-negative, nonmotile, nonfermentative bacteria selected on the basis of lack of motility and gram reaction among 1,200 cultures isolated during aerobic storage of lamb carcasses. at the 80% similarity level (ssm), eight clusters were formed. strains in clusters 1 to 6 could be identified as members of the family moraxellaceae and, more specifically, as members of the psychrobacter-[moraxella] phenylpyruvica sub ... | 1992 | 1637162 |
| meningitis caused by psychrobacter immobilis in an infant. | psychrobacter immobilis was isolated from the cerebrospinal fluid (csf) and blood of a 2-day-old infant who appeared well except for a fever and a full anterior fontanelle. the infant was treated with antibiotics intravenously. after 48 h, he became afebrile and csf and blood cultures were negative; he was then discharged. after 96 h of incubation, csf and blood cultures yielded a gram-negative organism, p. immobilis. the child was readmitted to the hospital, and the same organism was again isol ... | 1991 | 1774332 |
| ocular infection caused by psychrobacter immobilis acquired in the hospital. | the name psychrobacter immobilis recently has been proposed for a group of chiefly psychrotrophic, aerobic, gram-negative, nonmotile, oxidase-positive coccobacilli commonly found associated with fish, processed meat, and poultry products. this article reports an ocular infection in a 12-day-old newborn who acquired the infection in the hospital, probably because of frequent manipulations in a closed aerated incubator. also, this report intends to alert microbiologists to opportunistic infections ... | 1990 | 2312690 |
| classification of the spoilage flora of fish, with special reference to shewanella putrefaciens. | one hundred and fifty-nine gram-negative strains isolated from refrigerated fish, taken from the baltic sea or swedish inland waters, together with 32 reference strains of shewanella, pseudomonas, aeromonas and alcaligenes, were phenotypically classified using 124 unit characters. data were processed by the simple matching (ssm) and jaccard (sj) coefficients, and unweighted pair group algorithm with arithmetic averages. fourteen clusters were defined at the 75% sj similarity level which correspo ... | 1990 | 2391295 |
| a numerical taxonomic study of non-motile non-fermentative gram-negative bacteria from foods. | a numerical taxonomic study using 155 unit characters was performed on 63 strains of gram-negative non-motile non-fermentative bacteria isolated from proteinaceous foods. similar bacteria from other sources and several pseudomonas strains from meat were included for reference purposes. three clusters were observed at 76% s which contained all the food strains. cluster 1 was composed entirely of acinetobacter strains including 17 isolated from foods that were provisionally identified with acineto ... | 1988 | 3209519 |
| changes in aerobic microflora of skin and gills of mediterranean sardines (sardina pilchardus) during storage in ice. | sardines from the adriatic sea were examined fresh and after 4 and 8 days of storage in ice. a total of 1500 strains isolated were identified from the gills and the surface of the fish. pseudomonadaceae, neisseriaceae, flavobacterium/cytophaga, enterobacteriaceae, coryneform bacteria and micrococcaceae were the most common bacteria in fresh fish. during storage the pseudomonads (mainly the non-fluorescent strains) increased and became the dominating microflora; the neisseriaceae (moraxella, psyc ... | 1988 | 3275304 |
| cultural and chemical characterization of cdc groups eo-2, m-5, and m-6, moraxella (moraxella) species, oligella urethralis, acinetobacter species, and psychrobacter immobilis. | we determined phenotypic characteristics, cellular fatty acid composition, and isoprenoid quinone content of representative strains of cdc groups eo-2, m-5, and m-6, moraxella (moraxella) species, oligella urethralis, acinetobacter species, and psychrobacter immobilis. all organisms contained ubiquinone with eight isoprene units as the major isoprenolog, but distinct differences were observed in fatty acid composition. twenty-eight of the original collection of cdc group eo-2 strains were furthe ... | 1988 | 3356788 |
| relationship of cdc group eo-2 and psychrobacter immobilis. | thirty strains of microorganisms previously classified in the cdc group eo-2 were studied to determine the relationship of this group and the recently proposed psychrobacter immobilis. results of transformation studies indicated that 9 of the 30 studied strains should be reclassified as p. immobilis. while transformation studies should be used to identify the psychrobacter genus definitively, some presumptive characteristics useful in differentiating the cdc group eo-2 and p. immobilis were obse ... | 1987 | 3667912 |
| phylogenetic relationships between some members of the genera neisseria, acinetobacter, moraxella, and kingella based on partial 16s ribosomal dna sequence analysis. | we obtained 16s ribosomal dna (rdna) sequence data for strains belonging to 11 species of proteobacteria, including the type strains of kingella kingae, neisseria lactamica, neisseria meningitidis, moraxella lacunata subsp. lacunata, [neisseria] ovis, moraxella catarrhalis, moraxella osloensis, [moraxella] phenylpyruvica, and acinetobacter lwoffii, as well as strains of neisseria subflava and acinetobacter calcoaceticus. the data in a distance matrix constructed by comparing the sequences suppor ... | 1994 | 7520730 |
| corrigendum to "cloning, sequence and structural features of a lipase from the antarctic facultative psychrophile psychrobacter immobilis b10" [biochim. biophys. acta 1171 (1993) 331-333]. | | 1995 | 7632728 |
| comparison of antibacterial activity in the hemocytes of different crustacean species. | antibacterial activity in hemocytes of the squat lobster, galathea strigosa, the norway lobster, nephrops norvegicus, the common shrimp, crangon crangon, and the giant antarctic isopod, glyptonotus antarcticus, was investigated in vitro. for all species, the marine bacterium, psychrobacter immobilis, was used as the test organism, although with g. antarcticus, the gram positive bacteria, planococcus citreus and bs 68 (an isolate from antarctic waters), were also used. hemocyte lysate supernatant ... | 1995 | 7866773 |
| cloning, sequence and structural features of a lipase from the antarctic facultative psychrophile psychrobacter immobilis b10. | a lipase gene (lip1) from the facultative psychrophilic strain psychrobacter immobilis b10 has been cloned and sequenced. the deduced preprotein sequence is composed of 317 amino acids with a predicted m(r) of 35,288. a primary structure alignment of lipases including lip1 shows conserved elements for which a structural role is proposed in the light of recent crystallographic studies. the analysis of the psychrophilic enzyme sequence suggests characteristics in relation with the adaptation to co ... | 1993 | 7916627 |
| fatal psychrobacter immobilis infection in a patient with aids. | | 1994 | 7986423 |
| relatedness of three species of "false neisseriae," neisseria caviae, neisseria cuniculi, and neisseria ovis, by dna-dna hybridizations and fatty acid analysis. | dna-dna hybridization was used to determine the levels of genomic relatedness of the three species of "false neisseriae," neisseria caviae, neisseria cuniculi, and neisseria ovis. the reference strains of these species exhibited high levels of intraspecies relatedness (93 to 100% for n. caviae, 79 to 100% for n. cuniculi, and 68 to 100% for n. ovis) but low levels of interspecific relatedness (less than 34%) to each other and to various species belonging to the beta subclass of the proteobacteri ... | 1993 | 8494736 |
| the beta-lactamase secreted by the antarctic psychrophile psychrobacter immobilis a8. | a class c beta-lactamase has been purified from the culture supernatant of the antarctic psychrophile psychrobacter immobilis a8. this psychrophilic beta-lactamase displays a low level of thermal stability and a low optimal temperature of activity. in contrast to other cold-adapted enzymes, its level of specific activity is not higher than that of mesophilic class c beta-lactamases. | 1995 | 8534113 |
| structural organization of a bacillus subtilis operon encoding menaquinone biosynthetic enzymes. | menaquinone (mk) is a non-protein component of the bacillus subtilis (bs) electron transport chain synthesized from chorismate through a series of mk-specific reactions. the genes encoding biosynthesis of the naphthoquinone ring of mk are clustered at 273 degrees on the bs chromosome. a 3.9-kb region capable of rescuing men mutants blocked in the early stages of mk biosynthesis was sequenced and found to contain three major open reading frames (orfs). the first orf (menf) has a predicted size of ... | 1995 | 8566759 |
| novel psychrobacter species from antarctic ornithogenic soils. | ornithogenic soil is derived from the deposition of the fecal matter of various species of birds and is a major source of nutrient input in the antarctic marine ecosystem. a significant proportion of microbiota of ornithogenic soil collected from an adélie penguin colony in eastern antarctica (vestfold hills ice-free zone) consisted of gram-negative, coccoid bacteria identified on the basis of their phospholipid ester-linked fatty acid and lipid class profiles as psychrobacter strains. phenotypi ... | 1996 | 8863407 |
| enzymes from cold-adapted microorganisms. the class c beta-lactamase from the antarctic psychrophile psychrobacter immobilis a5. | a heat-labile beta-lactamase has been purified from culture supernatants of psychrobacter immobilis a5 grown at 4 degrees c and the corresponding chromosomal ampc gene has been cloned and sequenced. all structural and kinetic properties clearly relate this enzyme to class c beta-lactamases. the kinetic parameters of p. immobilis beta-lactamase for the hydrolysis of some beta-lactam antibiotics are in the same range as the values recorded for the highly specialized cephalosporinases from pathogen ... | 1997 | 9063463 |
| diversity and association of psychrophilic bacteria in antarctic sea ice. | the bacterial populations associated with sea ice sampled from antarctic coastal areas were investigated by use of a phenotypic approach and a phylogenetic approach based on genes encoding 16s rrna (16s rdna). the diversity of bacteria associated with sea ice was also compared with the bacterial diversity of seawater underlying sea ice. psychrophilic (optimal growth temperature, < or = 15 degrees c; no growth occurring at 20 degrees c) bacterial diversity was found to be significantly enriched i ... | 1997 | 9251193 |
| cloning and characterization of the gene encoding psppi methyltransferase from the antarctic psychrotroph psychrobacter sp. strain ta137. predicted interactions with dna and organization of the variable region. | the gene (psppim) encoding the psppi dna methyltransferase (mtase) associated with the psppi restriction-modification (r-m) system (5'-ggncc-3') of psychrobacter species ta137 has been cloned and expressed in e. coli, and its nucleotide (nt) sequence has been determined. the coding region was 1248 nt in length and capable of specifying a 46826-da protein of 416 amino acids (aa). the predicted sequence of the mtase protein displays ten sequence motifs characteristic of all prokaryotic m5c-mtases ... | 1997 | 9332385 |
| styelins, broad-spectrum antimicrobial peptides from the solitary tunicate, styela clava. | two novel phenylalanine-rich antimicrobial peptides, styelin a and styelin b, were purified from the hemocytes of styela clava. the peptides had very similar masses (styelin a, 3685.8; styelin b, 3700.6) and amino acid compositions, and at least 17 of their first 20 n-terminal residues were identical. both styelins were effective against a panel of gram negative and gram positive bacterial pathogens of humans, usually acting with minimal inhibitory concentrations < 1.5 microgram/ml (< 0.5 microm ... | 1997 | 9467865 |
| phylogeny of the family moraxellaceae by 16s rdna sequence analysis, with special emphasis on differentiation of moraxella species. | thirty-three strains previously classified into 11 species in the bacterial family moraxellaceae were subjected to phylogenetic analysis based on 16s rrna sequences. the family moraxellaceae formed a distinct clade consisting of four phylogenetic groups as judged from branch lengths, bootstrap values and signature nucleotides. group i contained the classical moraxellae and strains of the coccal moraxellae, previously known as branhamella, with 16s rrna similarity of > or = 95%. a further divisio ... | 1998 | 9542079 |
| predictive models as means to quantify the interactions of spoilage organisms. | the purpose of this paper is to quantify the interactions of some groups of spoilage organisms that can be usually found in refrigerated meat stored in air, such as: enterobacteriaceae, pseudomonas, acinetobacter, psychrobacter, shewanella, carnobacterium, lactobacillus, leuconostoc, brochothrix and kurthia spp. the growth of these organisms was studied in the range of temperature 2-11 degrees c and ph 5.2-6.4, which is characteristic of refrigerated meat. the main growth parameters (maximum spe ... | 1998 | 9631338 |
| use of green fluorescent protein to tag and investigate gene expression in marine bacteria. | two broad-host-range vectors previously constructed for use in soil bacteria (a. g. matthysse, s. stretton, c. dandie, n. c. mcclure, and a. e. goodman, fems microbiol. lett. 145:87-94, 1996) were assessed by epifluorescence microscopy for use in tagging three marine bacterial species. expression of gfp could be visualized in vibrio sp. strain s141 cells at uniform levels of intensity from either the lac or the npt-2 promoter, whereas expression of gfp could be visualized in psychrobacter sp. st ... | 1998 | 9647829 |
| cell surface analysis techniques: what do cell preparation protocols do to cell surface properties? | cell surface analysis often requires manipulation of cells prior to examination. the most commonly employed procedures are centrifugation at different speeds, changes of media during washing or final resuspension, desiccation (either air drying for contact angle measurements or freeze-drying for sensitive spectroscopic analysis, such as x-ray photoelectron spectroscopy), and contact with hydrocarbon (hydrophobicity assays). the effects of these procedures on electrophoretic mobility, adhesion to ... | 1999 | 10388679 |
| nadp+-dependent glutamate dehydrogenase in the antarctic psychrotolerant bacterium psychrobacter sp. tad1. characterization, protein and dna sequence, and relationship to other glutamate dehydrogenases. | the antarctic psychrotolerant bacterium psychrobacter sp. tad1 contains two distinct glutamate dehydrogenases (gdh), each specific for either nadp+ or nad+. this feature is quite unusual in bacteria, which generally have a single gdh. nadp+-dependent gdh has been purified to homogeneity and the gene encoding gdh has been cloned and expressed. the enzyme has a hexameric structure. the amino acid sequence determined by peptide and gene analyses comprises 447 residues, yielding a protein with a mol ... | 2000 | 10601858 |
| psychrobacters and related bacteria in freshwater fish. | three phenotypic identification systems were employed to identify 106 strains of gram-negative, nonmotile, aerobic bacteria obtained during iced storage of wild (salmo trutta and esox lucius) and farmed (oncorhynchus mykiss) freshwater fish. using diagnostic tables and computer-assisted identification, the isolates were psychrobacter (64 strains), acinetobacter (24 strains), moraxella (6 strains), chryseobacterium (5 strains), myroides odoratus (2 strains), flavobacterium (1 strain), empedobacte ... | 2000 | 10716558 |
| development of a new lysis solution for releasing genomic dna from bacterial cells for dna amplification by polymerase chain reaction. | a new lysis solution designated tz, consisting of 2.0% triton x-100 plus 2.5 mg sodium azide/ml in 0.1 m tris-hcl buffer at ph 8.0, yielded higher levels of genomic dna from escherichia coli o157:h7 cells compared with a number of other commonly used cell lysis methods. ethidium bromide stained dna bands resulting from pcr amplification of target dna from 100 cfu of e. coli o157:h7 were readily detected following electrophoresis of agarose gels. in contrast, conventional cell lysis methods faile ... | 2000 | 10756522 |
| phylogenetic analysis of psychrophilic bacteria isolated from the japan trench, including a description of the deep-sea species psychrobacter pacificensis sp. nov. | phylogenetic positions of psychrophilic bacteria isolated from the japan trench were determined by sequencing analysis of pcr-amplified bacterial small subunit (16s) rrna genes. between surface and deep-sea psychrophiles, distinct positions clearly differed within the gamma-proteobacteria. in phylogenetic analysis using neighbour-joining, maximum-parsimony and maximum-likelihood, strains from surface seawater were inferred to be located in the halomonas aquamarina-meridiana clade within the fami ... | 2000 | 10758895 |
| simultaneous direct counting of total and specific microbial cells in seawater, using a deep-sea microbe as target. | to rapidly and accurately enumerate total and specific microbes in aquatic samples, fluorescent in situ hybridization was combined with direct counting via direct immobilization of cells on a polymer-coated nuclepore filter. the technique, named fish-dc, achieved almost complete recovery of total cells and reproducibility of psychrobacter pacificensis cells of deep-sea origin (error, </=3%) in a mixed culture and in natural seawater. target cells immobilized on the filter were also successfully ... | 2000 | 10788402 |
| a rolling-circle plasmid from psychrobacter sp. ta144: evidence for a novel rep subfamily. | in this paper we report the cloning and sequencing of two small plasmids, ptaup and ptadw, from the antarctic gram-negative psychrobacter sp strain ta144. the observation that ptaup contains a putative rep-coding gene (psyrep) suggested that its duplication occurs via a rolling-circle replication mechanism. this hypothesis was confirmed by the identification of the ptaup single-stranded dna form. the putative ptaup plus origin of replication was found at the 3' end of the psyrep by using an in v ... | 2000 | 10913365 |
| phylogenetic analysis of culturable dimethyl sulfide-producing bacteria from a spartina-dominated salt marsh and estuarine water. | dimethylsulfoniopropionate (dmsp), an abundant osmoprotectant found in marine algae and salt marsh cordgrass, can be metabolized to dimethyl sulfide (dms) and acrylate by microbes having the enzyme dmsp lyase. a suite of dms-producing bacteria isolated from a salt marsh and adjacent estuarine water on dmsp agar plates differed markedly from the pelagic strains currently in culture. while many of the salt marsh and estuarine isolates produced dms and methanethiol from methionine and dimethyl sulf ... | 2001 | 11229912 |
| molecular characterization of a recombinant replication protein (rep) from the antarctic bacterium psychrobacter sp. ta144. | the antarctic gram-negative bacterium psychrobacter sp. ta144 contains two small cryptic plasmids, called ptaup and ptadw. ptaup encodes a replication enzyme (psyrep) whose activity is responsible for plasmid replication via the rolling circle replication pathway. several attempts to produce the wild-type biologically active psyrep in escherichia coli failed, possibly due to auto-regulation of the protein population. however, the serendipitous occurrence of a frameshift mutation during the prepa ... | 2001 | 11325553 |
| psychrobacter proteolyticus sp. nov., a psychrotrophic, halotolerant bacterium isolated from the antarctic krill euphausia superba dana, excreting a cold-adapted metalloprotease. | an antarctic marine bacterium (strain 116) excreting an extracellular cold-adapted metalloprotease was subjected to a detailed polyphasic taxonomic investigation. strain 116 was previously isolated from the stomach of a specimen of the antarctic krill euphasia superba dana and tentatively characterized as sphingomonas paucimobilis 116. the 16s rdna sequence analysis showed that the strain is in fact related to species of the genus psychrobacter, next to psychrobacter glacincola (97.4% similarity ... | 2001 | 11403398 |
| bacterial deposition in a parallel plate and a stagnation point flow chamber: microbial adhesion mechanisms depend on the mass transport conditions. | deposition onto glass in a parallel plate (pp) and in a stagnation point (sp) flow chamber of marinobacter hydrocarbonoclasticus, psychrobacter sp. and halomonas pacifica, suspended in artificial seawater, was compared in order to determine the influence of methodology on bacterial adhesion mechanisms. the three strains had different cell surface hydrophobicities, with water contact angles on bacterial lawns ranging from 18 to 85 degrees. bacterial zeta potentials in artificial seawater were ess ... | 2002 | 11832522 |
| the antarctic psychrobacter sp. tad1 has two cold-active glutamate dehydrogenases with different cofactor specificities. characterisation of the nad+-dependent enzyme. | psychrobacter sp. tad1 is a psychrotolerant bacterium from antarctic frozen continental water that grows from 2 to 25 degrees c with optimal growth rate at 20 degrees c. the new isolate contains two glutamate dehydrogenases (gdh), differing in their cofactor specificities, subunit sizes and arrangements, and thermal properties. nadp+-dependent gdh is a hexamer of 47 kda subunits and it is comparable to other hexameric gdhs of family-i from bacteria and lower eukaria. the nad+-dependent enzyme, d ... | 2002 | 11867281 |
| change in the composition of the microflora on vacuum-packaged beef during chiller storage. | five fresh beefcut samples were divided into 10 pieces, respectively, vacuum-packaged and stored at 2 degrees c for up to 6 weeks. the average ph values of five pieces from five beef samples were 5.62 +/- 0.04 at the start of storage and 5.12 +/- 0.07 after 6 weeks of storage. the pieces were homogenized, diluted and cultivated at weekly intervals on glucose-blood-liver (bl), de man rogosa and sharpe (mrs) and trypticase soya (ts) agars at 7 degrees c for 10-14 days. from plates with 30-300 colo ... | 2002 | 11929174 |
| psychrobacter faecalis sp. nov., a new species from a bioaerosol originating from pigeon faeces. | the taxonomy of strain iso-46t isolated from a bioaerosol generated by cleaning of a pigeon faeces contaminated room was investigated in a polyphasic approach. the beige pigmented gram-negative, oxidase-negative organism contained a quinone system with mainly ubiquinone q-8, and the polar lipid profile was composed of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol, beside some hitherto uncharacterized phospholipids. major polyamines were spermidine and putrescine and a ... | 2002 | 12086186 |
| psychrobacter submarinus sp. nov. and psychrobacter marincola sp. nov., psychrophilic halophiles from marine environments. | two novel psychrophilic, halophilic, psychrobacter-like bacteria, strains kmm 225t and kmm 277t, were isolated from sea water and the internal tissues of an ascidian polysyncraton sp. specimen, respectively, and characterized using a polyphasic approach, which included phenotypic, genotypic, chemotaxonomic and phylogenetic analyses. the novel marine isolates were gram-negative, aerobic, coccoid, oxidase- and catalase-positive, non-pigmented, non-motile, psychrophilic and halophilic and they util ... | 2002 | 12148642 |
| limited selection of sodium channel blocking toxin-producing bacteria from paralytic shellfish toxin-contaminated mussels (aulacomya ater). | paralytic shellfish toxins (psts) are sodium channel blocking (scb) toxins, produced by cyanobacteria, as well as by marine dinoflagellates and their associated bacteria, and cause serious health and economic concern worldwide. in a previous study, approximately 70% of the bacteria enriched from pst-contaminated shellfish tissue and isolated on marine agar medium were observed to produce scb toxins. in the study reported here, the high percentage of cultivable toxigenic bacteria is demonstrated ... | 2002 | 12238500 |
| paracoccus yeeii sp. nov. (formerly cdc group eo-2), a novel bacterial species associated with human infection. | cdc eugonic oxidizer group 2 (eo-2) is a group of unclassified gram-negative bacterial strains isolated from various human sources. as determined by biochemical tests and analyses of fatty acid compositions, these organisms form a homogeneous group that appears to be distinct from but related to other paracoccus species. molecular studies were performed on a set of 13 eo-2 strains from various clinical sources and geographic locations in the united states and canada to determine their relationsh ... | 2003 | 12624070 |
| a novel h-ns-like protein from an antarctic psychrophilic bacterium reveals a crucial role for the n-terminal domain in thermal stability. | we describe here new members of the h-ns protein family identified in a psychrotrophic acinetobacter spp. bacterium collected in siberia and in a psychrophilic psychrobacter spp. bacterium collected in antarctica. both are phylogenetically closely related to the hvra and spb rhodobacter transcriptional regulators. their amino acid sequence shares 40% identity, and their predicted secondary structure displays a structural and functional organization in two modules similar to that of h-ns in esche ... | 2003 | 12637536 |
| psychrobacter pulmonis sp. nov., isolated from the lungs of lambs. | unusual gram-negative, catalase- and oxidase-positive, coccus-shaped bacteria isolated from the lungs of two lambs were characterized by phenotypic and molecular-genetic methods. comparative 16s rrna gene sequencing studies demonstrated that the unknown isolates were genealogically highly related to each other (99.8% sequence similarity) and represent a novel subline within the genus psychrobacter. the unknown bacterium was phylogenetically closely related to, but distinct from, psychrobacter ph ... | 2003 | 12710606 |
| psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional korean fermented seafood. | two gram-negative, non-motile, non-spore-forming and moderately halophilic cocci (strains ykj-103t and ykj-105) were isolated from the traditional korean fermented seafood, jeotgal. the two strains grew optimally at 25-30 degrees c and grew at 4 and 36 degrees c, but not above 37 degrees c. they grew in the presence of 0-10% (w/v) nacl with an optimum of 2-3% (w/v) nacl. strains ykj-103t and ykj-105 were chemotaxonomically characterized by having ubiquinone-8 (0-8) as the predominant isoprenoid ... | 2003 | 12710611 |
| origin and spoilage potential of the microbiota dominating genus psychrobacter in sterile rehydrated salt-cured and dried salt-cured cod (gadus morhua). | salt-cured and dried salt-cured cod rehydrated using sterile water and equipment have a short shelf life at 4 degrees c due to high bacterial counts. the microbiota develops off-odours which partly can be described as musty, causing sensory rejection within 7-10 days of chilled storage. the microbiota composition was studied in a total of 38 samples obtained from 10 different, both commercial and laboratory produced, salt-cured and dried salt-cured cod products. the dominating bacterium, represe ... | 2003 | 12781940 |
| characterization of several psychrobacter strains isolated from antarctic environments and description of psychrobacter luti sp. nov. and psychrobacter fozii sp. nov. | eleven psychrophilic bacteria isolated from antarctic coastal marine environments were subjected to a polyphasic taxonomic study. the isolates were oxidase-positive, halotolerant, gram-negative, non-motile coccobacilli with a strictly oxidative metabolism. the dna g + c content ranged from 44 to 47 mol%. dna-dna hybridization experiments showed six homology groups, two of them related at the species level to the type strain of psychrobacter immobilis, lmg 7203t (70-83%). the highest dna relatedn ... | 2003 | 12892132 |
| the microbial ecology of processing equipment in different fish industries-analysis of the microflora during processing and following cleaning and disinfection. | the microflora adhering to the processing equipment during production and after cleaning and disinfecting procedures was identified in four different processing plants. a total of 1009 microorganisms was isolated from various-agar plates and identified. a stepwise procedure using simple phenotypic tests was used to identify the isolates and proved a fast way to group a large collection of microorganisms. pseudomonas, neisseriaceae, enterobactericeae, coryneform, acinetobacter and lactic acid bac ... | 2003 | 14527796 |
| supercooled water brines within permafrost-an unknown ecological niche for microorganisms: a model for astrobiology. | this study describes brine lenses (cryopegs) found in siberian permafrost derived from ancient marine sediment layers of the arctic ocean. the cryopegs were formed and isolated from sediment ~100,000-120,000 years ago. they remain liquid at the in situ temperature of -10 degrees c as a result of their high salt content (170-300 g/l). [(14)c] glucose is taken up by the cryopeg biomass at -15 degrees c, indicating microbial metabolism at low temperatures in this habitat. furthermore, aerobic, anae ... | 2003 | 14577882 |
| diversity and structure of bacterial communities in arctic versus antarctic pack ice. | a comprehensive assessment of bacterial diversity and community composition in arctic and antarctic pack ice was conducted through cultivation and cultivation-independent molecular techniques. we sequenced 16s rrna genes from 115 and 87 pure cultures of bacteria isolated from arctic and antarctic pack ice, respectively. most of the 33 arctic phylotypes were >97% identical to previously described antarctic species or to our own antarctic isolates. at both poles, the alpha- and gamma-proteobacteri ... | 2003 | 14602620 |
| psychrobacter okhotskensis sp. nov., a lipase-producing facultative psychrophile isolated from the coast of the okhotsk sea. | a facultatively psychrophilic bacterium, strain md17(t), which hydrolyses lipids at 5 degrees c, was isolated from the monbetsu coast of the okhotsk sea in hokkaido, japan, when ice carried by the cold current came to the area. the isolate is an aerobic, non-motile coccobacillus that reduces nitrate to nitrite and hydrolyses tweens 20, 40, 60 and 80, but not gelatin, dna or alginic acid. the isolate grows at 0 degrees c, but not at temperatures higher than 36 degrees c; its optimum growth temper ... | 2003 | 14657134 |
| cold-active esterase from psychrobacter sp. ant300: gene cloning, characterization, and the effects of gly-->pro substitution near the active site on its catalytic activity and stability. | the gene encoding an esterase (psyest) of psychrobacter sp. ant300, a psychrophilic bacterium isolated from antarctic soil, was cloned, sequenced, and expressed in escherichia coli. psyest, which is a member of hormone-sensitive lipase (hsl) group of the lipase/esterase family, is a cold-active, themolabile enzyme with high catalytic activity at low temperatures (5-25 degrees c), low activation energy (e.g., 4.6 kcal/mol for hydrolysis of p-nitrophenyl butyrate), and a t(1/2) value of 16 min for ... | 2004 | 14726205 |
| role of bacteria in mediating the oviposition responses of aedes albopictus (diptera: culicidae). | the responses of aedes albopictus to sources of oviposition attractants and stimulants were evaluated with a behavioral bioassay in which females attracted to odorants emanating from water were trapped on screens coated with an adhesive. gravid mosquitoes were attracted to volatiles from larval-rearing water and soil-contaminated cotton towels. bacteria were isolated from these substrates and from an organic infusion made with oak leaves. through fatty acid-methyl ester analyses, six bacterial i ... | 2003 | 14765661 |
| the effect of dissolved organic carbon on bacterial adhesion to conditioning films adsorbed on glass from natural seawater collected during different seasons. | adhesion of three marine bacterial strains, i.e. marinobacter hydrocarbonoclasticus, psychrobacter sp. and halomonas pacifica with different cell surface hydrophobicities was measured on glass in a stagnation point flow chamber. prior to bacterial adhesion, the glass surface was conditioned for 1 h with natural seawater collected at different seasons in order to determine the effect of seawater composition on the conditioning film and bacterial adhesion to it. the presence of a conditioning film ... | 2003 | 14768468 |
| a new isolation method for labyrinthulids using a bacterium, psychrobacter phenylpyruvicus. | a new isolation method for labyrinthulids, marine microbes with spindle-shaped vegetative cells and gliding movement, is presented. the method for isolating labyrinthulids has been found to be more difficult and less reproducible than that for thraustochytrids, classified in the same order. so far serum seawater agar fortified with antibiotics has been proposed to be the best for isolation of labyrinthulids. the method presented here involves placing plant samples on an agar medium on which a ma ... | 2001 | 14961392 |
| molecular and phenotypic characterization of nonmotile gram-negative bacteria associated with spoilage of freshwater fish. | characterization of nonmotile bacteria associated with freshwater fish spoilage and that phenotypically resembled psychrobacter spp. | 2004 | 15012828 |
| relationship of critical temperature to macromolecular synthesis and growth yield in psychrobacter cryopegella. | most microorganisms isolated from low-temperature environments (below 4 degrees c) are eury-, not steno-, psychrophiles. while psychrophiles maximize or maintain growth yield at low temperatures to compensate for low growth rate, the mechanisms involved remain unknown, as does the strategy used by eurypsychrophiles to survive wide ranges of temperatures that include subzero temperatures. our studies involve the eurypsychrophilic bacterium psychrobacter cryopegella, which was isolated from a brin ... | 2004 | 15060036 |
| multiple linear regression analysis of bacterial deposition to polyurethane coatings after conditioning film formation in the marine environment. | many studies have shown relationships of substratum hydrophobicity, charge or roughness with bacterial adhesion, although bacterial adhesion is governed by interplay of different physico-chemical properties and multiple regression analysis would be more suitable to reveal mechanisms of bacterial adhesion. the formation of a conditioning film of organic compounds adsorbed from seawater affects the properties of substratum surfaces prior to bacterial adhesion, which is a complicating factor in stu ... | 2004 | 15184564 |
| [bacteria of the genus psychrobacter isolated from black sea water]. | | 2004 | 15198042 |
| recombinant protein production in antarctic gram-negative bacteria. | this review reports some results from our laboratory on the setting up of a psychrophilic expression system for the homologous/heterologous protein production in cold-adapted bacteria by using natural plasmids as cloning vectors. by screening some antarctic bacteria for the presence of extrachromosomal elements, we identified three new plasmids, pmtbl from pseudoalteromonas haloplanktis tac125, and ptaup and ptadw, from psychrobacter sp. ta144. the latter autoreplicating elements were isolated, ... | 2004 | 15269427 |
| psychrobacter nivimaris sp. nov., a heterotrophic bacterium attached to organic particles isolated from the south atlantic (antarctica). | an aggregate-attached bacterium, strain 88/2-7, was isolated from samples of the southern ocean and investigated in a polyphasic approach. the novel marine isolate is an aerobic, gram-negative, oxidase- and catalase-positive, non-motile short rod and grows in form of cream-colored colonies. growth was observed at 5-35 degrees c. the bacterium tolerated concentrations of 0-13% (w/v) nacl and utilized a relatively restricted spectrum of carbon sources. the analysis of the fatty acids revealed 18:1 ... | 2004 | 15368844 |
| psychrobacter maritimus sp. nov. and psychrobacter arenosus sp. nov., isolated from coastal sea ice and sediments of the sea of japan. | four strains of gram-negative, aerobic, psychrotolerant, non-motile, non-pigmented bacteria were isolated from coastal sea-ice and sediment samples. these strains displayed the general chemotaxonomic and phenotypic features of members of the genus psychrobacter. 16s rrna gene sequencing positioned the three isolates kmm 3646t, kmm 3643 and kmm 3645 and isolate kmm 3659t in two distinct lineages within the genus psychrobacter, displaying less than 98.5 % sequence similarity to the type strains of ... | 2004 | 15388738 |
| psychrobacter salsus sp. nov. and psychrobacter adeliensis sp. nov. isolated from fast ice from adelie land, antarctica. | nine psychrotolerant bacteria were isolated from fast ice in the middle of geologie archipelago, adelie land, antarctica and were categorized into two groups, based on their sds-page profiles. representatives from each of the two groups, namely strains dd 48t and sj 14t exhibited phenotypic and chemotaxonomic characteristics confirming to the genus psychrobacter. the 16s rrna gene sequence indicated that the two isolates are closely related to each other and to the already reported fifteen speci ... | 2004 | 15612619 |
| psychrobacter alimentarius sp. nov., isolated from squid jeotgal, a traditional korean fermented seafood. | two gram-negative, non-motile, non-spore-forming, moderately halophilic strains, jg-100t and jg-102, were isolated from squid jeotgal, a traditional korean fermented seafood. the two strains grew optimally at 30 degrees c and in the presence of 2-3 % (v/w) nacl. strains jg-100t and jg-102 were characterized chemotaxonomically; they both had ubiquinone-8 as the predominant respiratory lipoquinone and c(18 : 1)omega9c as the major fatty acid. their dna g+c content was 44 mol%. strains jg-100t and ... | 2005 | 15653872 |
| incidence, radioresistance, and behavior of psychrobacter spp. in rabbit meat. | the relative incidence of psychrobacter spp. in rabbit meat, the radioresistance of these bacteria, and the growth of nonirradiated and irradiated psychrobacter isolates, alone and in coculture, during chilled storage of inoculated sterile rabbit meat was investigated. psychrobacter spp. accounted for 4.2% of the storage psychrotrophic flora of 30 rabbit carcasses. the radiation d10-values of 10 psychrobacter isolates, irradiated at 4 degrees c in minced rabbit meat, ranged from 0.8 to 2.0 kgy, ... | 2005 | 15771179 |
| psychrobacter cibarius sp. nov., isolated from jeotgal, a traditional korean fermented seafood. | two novel psychrobacter-like bacterial strains, jg-219(t) and jg-220, were isolated from jeotgal, a traditional korean fermented seafood. cells of strains jg-219(t) and jg-220 were gram-negative, non-motile coccobacilli. growth of the two strains was observed at 4-32 degrees c. they grew optimally in the presence of 2-5 % (w/v) nacl. strains jg-219(t) and jg-220 contained c(18 : 1)omega9c and c(17 : 1)omega8c as the major fatty acids and q-8 as the predominant ubiquinone. the dna g+c contents of ... | 2005 | 15774627 |
| psychrobacter vallis sp. nov. and psychrobacter aquaticus sp. nov., from antarctica. | twelve strains of psychrophilic bacteria were isolated from cyanobacterial mat samples collected from various water bodies in the mcmurdo dry valley region of antarctica. all the isolates were gram-negative, non-motile, coccoid, psychrophilic, halotolerant bacteria and had c(16 : 1)omega7c, c(17 : 1)omega8c and c(18 : 1)omega9c as the major fatty acids, ubiquinone-8 as the respiratory quinone and dna g+c content of 41-46 mol%. based on these characteristics, the isolates were assigned to the gen ... | 2005 | 15774658 |
| acquisition of resistance to carbapenems in multidrug-resistant clinical strains of acinetobacter baumannii: natural insertional inactivation of a gene encoding a member of a novel family of beta-barrel outer membrane proteins. | the outer membrane proteins responsible for the influx of carbapenem beta-lactam antibiotics in the nonfermentative gram-negative pathogen acinetobacter baumannii are still poorly characterized. resistance to both imipenem and meropenem in multidrug-resistant clinical strains of a. baumannii is associated with the loss of a heat-modifiable 29-kda outer membrane protein, designated caro. the chromosomal locus containing the caro gene was cloned and characterized from different clinical isolates. ... | 2005 | 15793123 |
| psychrobacter aquimaris sp. nov. and psychrobacter namhaensis sp. nov., isolated from sea water of the south sea in korea. | two gram-negative, non-motile, non-spore-forming, slightly halophilic bacterial strains, sw-210(t) and sw-242(t), were isolated from sea water of the south sea in korea, and were characterized taxonomically by means of a polyphasic approach. the two isolates grew optimally at 25-30 degrees c and in the presence of 2-3 % (w/v) nacl. strains sw-210(t) and sw-242(t) contained q-8 as the predominant respiratory lipoquinone and c(18 : 1)omega9c as the major fatty acid. the dna g+c contents of strains ... | 2005 | 15879226 |
| degradation of crude oil by an arctic microbial consortium. | the ability of a psychrotolerant microbial consortium to degrade crude oil at low temperatures was investigated. the enriched arctic microbial community was also tested for its ability to utilize various hydrocarbons, such as long-chain alkanes (n-c24 to n-c34), pristane, (methyl-)naphthalenes, and xylenes, as sole carbon and energy sources. except for o-xylene and methylnaphthalenes, all tested compounds were metabolized under conditions that are typical for contaminated marine liquid sites, na ... | 2005 | 15999222 |
| psychrobacter celer sp. nov., isolated from sea water of the south sea in korea. | a gram-negative, non-motile, non-spore-forming, slightly halophilic bacterial strain, sw-238(t), was isolated from sea water collected from the south sea in korea, and subjected to a polyphasic taxonomic study. the organism grew optimally at 25-30 degrees c and in the presence of 2-3% (w/v) nacl. strain sw-238(t) was characterized chemotaxonomically as containing ubiquinone (q-8) as the predominant respiratory lipoquinone and c(18:1)omega9c as the major fatty acid. its dna g+c content was 47.6 m ... | 2005 | 16166683 |
| cloning and sequence analysis of the esta gene encoding enzyme for producing (r)-beta-acetylmercaptoisobutyric acid from pseudomonas aeruginosa 1001. | the esta gene encoding the enzyme that catalyzes the production of (r)-beta-acetylmercaptoisobutyric acid from (r,s)-ester from pseudomonas aeruginosa 1001, was cloned in escherichia coli and its nucleotide sequence was determined, revealing the presumed open reading frame encoding a polypeptide of 316 amino acid residues (948 nucleotides). the overall a + t and c + g compositions were 32.59% and 67.41%, respectively. the amino acid sequence of the esta gene product showed a significant similari ... | 2000 | 16232934 |
| psychrobacter bacteraemia in a cirrhotic patient after the consumption of raw geoduck clam. | psychrobacter species rarely cause infections in human. we reported herein a 62-year-old cirrhotic patient who presented with fever and diarrhoea after the consumption of raw geoduck clam. blood culture grew psychrobacter phenylpyruvicus which was sensitive to most anti-microbial agents. the patient responded promptly to intravenous antibiotics. this is the first report of human infection with this unusual organism which illustrates the potential health hazards of eating raw geoduck clam in pati ... | 2006 | 16253331 |
| aerobic bacterial flora of the vagina and prepuce of california sea lions (zalophus californianus) and investigation of associations with urogenital carcinoma. | to investigate the association between genital bacterial infection and urogenital carcinoma in california sea lions (zalophus californianus), vaginal and preputial swabs for bacterial isolation were taken from 148 free-ranging and 51 stranded california sea lions including 16 animals with urogenital carcinoma. cytological examination of vaginal or preputial smears showed a majority (65.5%, 57/87) of animals examined had mild or no inflammation. aerobic bacteria were isolated from 116 (78.4%) wil ... | 2006 | 16326045 |
| characterization of potential stress responses in ancient siberian permafrost psychroactive bacteria. | past studies of cold-acclimated bacteria have focused primarily on organisms not capable of sub-zero growth. siberian permafrost isolates exiguobacterium sp. 255-15 and psychrobacter sp. 273-4, which grow at subzero temperatures, were used to study cold-acclimated physiology. changes in membrane composition and exopolysaccharides were defined as a function of growth at 24, 4 and -2.5 degrees c in the presence and absence of 5% nacl. as expected, there was a decrease in fatty acid saturation and ... | 2005 | 16329933 |
| molecular microbial diversity of a soil sample and detection of ammonia oxidizers from cape evans, mcmurdo dry valley, antarctica. | the aim of our study was to estimate the uncultured eubacterial diversity of a soil sample collected below a dead seal, cape evans, mcmurdo, antarctica by an ssu rdna gene library approach. our study by sequencing of clones from ssu rdna gene library approach revealed high diversity in the soil sample from antarctica. more than 50% of clones showed homology to cytophaga-flavobacterium-bacteroides group; sequences also belonged to alpha, beta, gamma proteobacteria, thermus-deinococcus and high gc ... | 2007 | 16517136 |
| psychrobacter cryohalolentis sp. nov. and psychrobacter arcticus sp. nov., isolated from siberian permafrost. | three gram-negative, non-motile, non-pigmented, oxidase-positive coccobacilli capable of growth at temperatures from -10 to 30 degrees c and salinities of 0 to 1.7 m nacl were isolated from siberian permafrost and characterized. both 16s rrna and gyrb gene sequencing studies placed the isolates in the gammaproteobacteria within the genus psychrobacter. however, with higher bootstrap values and reproducible tree topologies, gyrb represented a more reliable phylogenetic marker for the taxonomy of ... | 2006 | 16738105 |
| bacterial community in ancient siberian permafrost as characterized by culture and culture-independent methods. | the microbial composition of ancient permafrost sediments from the kolyma lowland of northeast eurasia was examined through culture and culture-independent approaches. these sediments have been continuously frozen for 5,000 to 2-3 million years. a total of 265 bacteria 16s rrna gene sequences were amplified from the permafrost total-community genomic dna and screened by amplified ribosomal 16s rrna restriction analysis. members of three major lineages were found: gamma-proteobacteria (mostly xan ... | 2006 | 16805696 |
| evaluation of pyrrolidonyl arylamidase for the identification of nonfermenting gram-negative rods. | to evaluate the activity of pyrrolidonyl arylamidase (pyr) for the differentiation and identification of nonfermenting gram negative rods (nfgnr), 293 isolates were tested. a 24 h culture of each test organism was prepared. from this a 108-109 cfu/ml suspension was added to 0.25 ml of sterile physiologic solution. a pyr disk was then added and the test was incubated for 30 minutes at 35-37 degrees c, at environmental atmosphere. reading was done by adding 1 drop of cinnamaldehyde reagent. strain ... | 2007 | 16822636 |
| evaluation of freeze-dried kefir coculture as starter in feta-type cheese production. | the use of freeze-dried kefir coculture as a starter in the production of feta-type cheese was investigated. maturation of the produced cheese at 4 degrees c was monitored for up to 70 days, and the effects of the starter culture, the salting method, and the ripening process on quality characteristics were studied. the use of kefir coculture as a starter led to increased lactic acid concentrations and decreased ph values in the final product associated with significantly higher conversion rates ... | 2006 | 16957238 |
| surgical wound infection associated with psychrobacter phenylpyruvicus-like organism. | a case of surgical wound infection caused by psychrobacter phenylpyruvicus-like organism is described. the strain showed phenotypic characteristics typical of p. phenylpyruvicus, but 16s rrna sequencing showed 98.2% relatedness to moraxella phenylpyruvica strain 752/52 and only 94.8% with p. phenylpyruvicus type strain atcc 23333(t). the results of molecular analysis suggest that the strain we isolated may represent a new species within the genus psychrobacter. | 2007 | 16989971 |
| effects of packaging systems on the natural microflora and acceptability of chicken breast meat. | the effect of 3 packaging systems on the spoilage microflora, objective color, and sensory characteristics of fresh commercial broiler chicken breast meat was investigated. fresh skinless and boneless chicken broiler breasts were purchased from a local poultry processing plant and packaged in either (1) a conventional styrofoam tray with polyvinyl chloride overwrap and absorbent pad, (2) a styrofoam tray with polyvinyl chloride overwrap minus absorbent pad, or (3) a fresh-r-pax (frp) container e ... | 2006 | 17012172 |
| predominance of roseobacter, sulfitobacter, glaciecola and psychrobacter in seawater collected off ushuaia, argentina, sub-antarctica. | bacterial diversity in sub-antarctic seawater, collected off ushuaia, argentina, was examined using a culture independent approach. the composition of the 16s rrna gene libraries from seawater and seawater contaminated with the water soluble fraction of crude oil was statistically different (p value 0.001). in both libraries, clones representing the alphaproteobacteria, gammaproteobacteria, the cytophaga-flavobacterium-bacteroidetes group and unculturable bacteria were dominant. clones associate ... | 2007 | 17026513 |
| lipolytic activity of antarctic cold-adapted marine bacteria (terra nova bay, ross sea). | the aim of this study was to investigate the lipolytic activity of cold-adapted antarctic marine bacteria and, furthermore, the combined effect of some environmental factors on this enzymatic process. | 2006 | 17040228 |
| proteomic analysis of psychrobacter cryohalolentis k5 during growth at subzero temperatures. | it is crucial to examine the physiological processes of psychrophiles at temperatures below 4 degrees c, particularly to facilitate extrapolation of laboratory results to in situ activity. using two dimensional electrophoresis, we examined patterns of protein abundance during growth at 16, 4, and -4 degrees c of the eurypsychrophile psychrobacter cryohalolentis k5 and report the first identification of cold inducible proteins (cips) present during growth at subzero temperatures. growth temperatu ... | 2007 | 17123128 |
| a proteomic analysis of psychrobacter articus 273-4 adaptation to low temperature and salinity using a 2-d liquid mapping approach. | psychrobacter 273-4 was isolated from a 20,000-40,000-year-old siberian permafrost core, which is characterized by low temperature, low water activity, and high salinity. to explore how 273-4 survives in the permafrost environment, proteins in four 273-4 samples cultured at 4 and 22 degrees c in media with and without 5% sodium chloride were profiled and comparatively studied using 2-d hplc and ms. the method used herein involved fractionation via a ph gradient using chromatofocusing followed by ... | 2007 | 17177241 |
| marine bacteria associated with the korean brown alga, undaria pinnatifida. | several marine bacterial strains were isolated from undaria pinnatifida (miyok in korean). sixty-six strains were isolated on r2a agar media at 10 degrees and identified by a phylogenetic analysis of the 16s rrna gene sequences. they were grouped into 10 different sequence types based on the initial sequence analysis of the 5' domain of the gene (approximately 500 bp). full sequences of 16s rrna gene were obtained from one strain in each sequence type and the species-affiliation was determined u ... | 2006 | 17205052 |
| identification of "haematobacter," a new genus of aerobic gram-negative rods isolated from clinical specimens, and reclassification of rhodobacter massiliensis as "haematobacter massiliensis comb. nov.". | twelve strains of gram-negative, nonfermenting rods recovered mainly from septicemic patients were studied using conventional and molecular methods. the phenotypic profiles of these strains most closely resembled psychrobacter phenylpyruvicus. they produced catalase, oxidase, urease, and h(2)s (lead acetate paper) but did not produce indole, reduce nitrate or nitrite, or hydrolyze gelatin or esculin. no acid production was observed in a king's oxidation-fermentation base containing d-glucose, d- ... | 2007 | 17287332 |
| eurythermalism and the temperature dependence of enzyme activity. | the "equilibrium model" has provided new tools for describing and investigating enzyme thermal adaptation. it has been shown that the effect of temperature on enzyme activity is not only governed by deltag(double dagger)(cat) and deltag(double dagger)(inact) but also by two new intrinsic parameters, deltah(eq) and t(eq), which describe the enthalpy and midpoint, respectively, of a reversible equilibrium between active and inactive (but not denatured) forms of enzyme. twenty-one enzymes from orga ... | 2007 | 17341686 |
| metabolic activity and phylogenetic diversity of reed (phragmites australis) periphyton bacterial communities in a hungarian shallow soda lake. | in the present study, the species composition and potential metabolic activities of bacterial communities of reed phragmites australis (cav.) (trin. ex steudel) periphyton from lake velencei were studied by cultivation-based and metabolic fingerprinting methods. serially diluted spring biofilm samples were used to test the community-level physiological profiling (clpp) using biolog microplates, and for plating onto different media. on the basis of their morphological, biochemical, and physiologi ... | 2007 | 17406774 |
| stability of the biodiversity of the surface consortia of gubbeen, a red-smear cheese. | a total of 1,052 bacteria and 828 yeasts were isolated from the surface flora of 6 batches of gubbeen cheese made in 1996-1997 and 2002-2003. stability of the microflora was evaluated over time and also during ripening at 4, 10, and 16 d (batches 4, 5, and 6) or at 4, 16, 23, and 37 d (batches 1, 2, and 3). bacteria were identified using pulsed-field gel electrophoresis, repetitive extragenic palindromic-pcr, and 16s rrna gene sequencing, and yeasts were identified by fourier transform infrared ... | 2007 | 17430918 |
| antibacterial and antilarval activity of deep-sea bacteria from sediments of the west pacific ocean. | deep-sea microorganisms are a new source of bioactive compounds. in this study, crude ethyl acetate extracts of 176 strains of deep-sea bacteria, isolated from sediments of the west pacific ocean, were screened for their antibacterial activity against four test bacterial strains isolated from marine biofilms. of these, 28 deep-sea bacterial strains exhibited antibacterial activity against one or more of the bacteria tested. active deep-sea bacterial strains belonged mainly to the genera of pseud ... | 2007 | 17453737 |
| microbial diversity of indian ocean hydrothermal vent plumes: microbes tolerant of desiccation, peroxide exposure, and ultraviolet and gamma-irradiation. | the microbial diversity of kali chimney plumes, part of a hydrothermal vent field in the rodriguez triple junction, indian ocean (depth approximately 2,240 m), was examined in an attempt to discover "extremotolerant" microorganisms that have evolved unique resistance capabilities to this harsh environment. water and sediment samples were collected from the vent and from sediments located at various distances (2-20 m) away from and surrounding the chimney. samples were screened for hypertolerant ... | 2007 | 17480169 |
| the diversity of bacterial communities associated with atlantic cod gadus morhua. | the spatial and temporal changes in the bacterial communities associated with the atlantic cod gadus morhua were investigated using terminal restriction fragment length polymorphism (t-rflp) analysis of 16s recombinant dna (rdna). epidermal mucous was sampled from 366 cod caught in three harvest locations (baltic, icelandic, and north seas) over three seasons (spring 2002, autumn 2002, and spring 2003), and an automated method for the high-throughput processing of environmental samples was devel ... | 2008 | 17624487 |
| production of volatile aroma compounds by bacterial strains isolated from different surface-ripened french cheeses. | twelve bacterial strains belonging to eight taxonomic groups: brevibacterium linens, microbacterium foliorum, arthrobacter arilaitensis, staphylococcus cohnii, staphylococcus equorum, brachybacterium sp., proteus vulgaris and psychrobacter sp., isolated from different surface-ripened french cheeses, were investigated for their abilities to generate volatile aroma compounds. out of 104 volatile compounds, 54 volatile compounds (identified using dynamic headspace technique coupled with gas chromat ... | 2007 | 17701035 |
| selection and characterization of aerobic bacteria capable of degrading commercial mixtures of low-ethoxylated nonylphenols. | isolation and characterization of new bacterial strains capable of degrading nonylphenol ethoxylates (npneo) with a low ethoxylation degree, which are particularly recalcitrant to biodegradation. | 2008 | 17850311 |
| biodegradative potential and characterization of psychrotolerant polychlorinated biphenyl-degrading marine bacteria isolated from a coastal station in the terra nova bay (ross sea, antarctica). | antarctic marine bacteria were screened for their ability to degrade polychlorinated biphenyls (pcb) as the sole carbon and energy source at both 4 degrees c and 15 degrees c. pcb-degrading isolates (7.1%) were identified by sequencing their 16s rdna as pseudoalteromonas, psychrobacter and arthrobacter members. one representative isolate per genera was selected for evaluating the biodegradative potential under laboratory scale and phenotypically characterized. removal of individual pcb congeners ... | 2007 | 17854841 |
| [phylogenetic diversity of culturable bacteria in the ancient salt deposits of the yipinglang salt mine, p. r. china]. | the microbial diversity of cultivable bacteria, isolated from the ancient salt deposits from the yipinglang salt mine (ypl) in the yunnan province, p. r. china,was investigated by using conventional culture-dependent method and phylogenetic analyses based on 16s rrna gene sequence comparisons. 38 bacteria strains were isolated from the brine, halite and saline soil samples on mba (marine broth agar 2216, difco) and isp 2 (international streotomyces project medium 2) media supplemented with 0.5-3 ... | 2007 | 17944352 |
| oil-utilizing bacteria associated with fish from the arabian gulf. | the objectives were to count and identify the oil-utilizing bacteria associated with fish, and to study their hydrocarbon-degradation potential. | 2007 | 17953689 |