| antibiotic resistance in neisseria denitrificans. | an ampicillin-resistant strain of neisseria denitrificans was produced by serial passage of the organisms in media containing increased concentrations of antibiotic. the 400-fold increase in resistance obtained was a relatively stable characteristic. ampicillin resistance in this organism was apparently related to a loss or modification of the penicillin-binding proteins associated with the cytoplasmic membranes. membranes isolated from the ampicillin-resistant strain bound significantly less ra ... | 1980 | 6901594 |
| isolation and characterization of phenol-degrading denitrifying bacteria. | phenol is a man-made as well as a naturally occurring aromatic compound and an important intermediate in the biodegradation of natural and industrial aromatic compounds. whereas many microorganisms that are capable of aerobic phenol degradation have been isolated, only a few phenol-degrading anaerobic organisms have been described to date. in this study, three novel nitrate-reducing microorganisms that are capable of using phenol as a sole source of carbon were isolated and characterized. phenol ... | 1998 | 9647811 |
| structural and evolutionary inference from molecular variation in neisseria porins. | the porin proteins of the pathogenic neisseria species, neisseria gonorrhoeae and neisseria meningitidis, are important as serotyping antigens, putative vaccine components, and for their proposed role in the intracellular colonization of humans. a three-dimensional structural homology model for neisseria porins was generated from escherichia coli porin structures and n. meningitidis pora and porb sequences. the neisseria sequences were readily assembled into the 16-strand beta-barrel fold charac ... | 1999 | 10225902 |
| chloromethane utilization gene cluster from hyphomicrobium chloromethanicum strain cm2(t) and development of functional gene probes to detect halomethane-degrading bacteria. | hyphomicrobium chloromethanicum cm2(t), an aerobic methylotrophic member of the alpha subclass of the class proteobacteria, can grow with chloromethane as the sole carbon and energy source. h. chloromethanicum possesses an inducible enzyme system for utilization of chloromethane, in which two polypeptides (67-kda cmua and 35-kda cmub) are expressed. previously, four genes, cmua, cmub, cmuc, and puru, were shown to be essential for growth of methylobacterium chloromethanicum on chloromethane. the ... | 2001 | 11133460 |
| use of stable-isotope probing, full-cycle rrna analysis, and fluorescence in situ hybridization-microautoradiography to study a methanol-fed denitrifying microbial community. | a denitrifying microbial consortium was enriched in an anoxically operated, methanol-fed sequencing batch reactor (sbr) fed with a mineral salts medium containing methanol as the sole carbon source and nitrate as the electron acceptor. the sbr was inoculated with sludge from a biological nutrient removal activated sludge plant exhibiting good denitrification. the sbr denitrification rate improved from less than 0.02 mg of no(3)(-)-n mg of mixed-liquor volatile suspended solids (mlvss)(-1) h(-1) ... | 2004 | 14711691 |
| evaluation of conventional and real-time pcr assays using two targets for confirmation of results of the cobas amplicor chlamydia trachomatis/neisseria gonorrhoeae test for detection of neisseria gonorrhoeae in clinical samples. | two conventional pcr-enzyme immunoassays (pcr-eias) and two real-time pcr assays (lightcycler system; roche diagnostics) were evaluated as confirmation assays with cppb and 16s rrna genes as targets. of 765 male and female genitourinary and nasopharyngeal specimens positive for neisseria gonorrhoeae in the cobas amplicor chlamydia trachomatis/neisseria gonorrhoeae pcr test (roche diagnostics), 229 (30%) were confirmed positive; 13 of these (5.7%) were lacking the cppb gene. of the 534 samples (7 ... | 2005 | 15872247 |
| identification of candidate periodontal pathogens and beneficial species by quantitative 16s clonal analysis. | most studies of the bacterial etiology of periodontitis have used either culture-based or targeted dna approaches, and so it is likely that pathogens remain undiscovered. the purpose of this study was to use culture-independent, quantitative analysis of biofilms associated with chronic periodontitis and periodontal health to identify pathogens and beneficial species. samples from subjects with periodontitis and controls were analyzed using ribosomal 16s cloning and sequencing. several genera, ma ... | 2005 | 16081935 |
| specific and sensitive detection of neisseria gonorrhoeae in clinical specimens by real-time pcr. | early diagnosis of neisseria gonorrhoeae infections is important with regard to patients' health and infectivity. we report the development of a specific and sensitive taqman assay for the detection of n. gonorrhoeae in clinical samples. the target sequence is a 76-bp fragment of the 5' untranslated region of the opa genes that encode opacity proteins. a panel of 448 well-defined n. gonorrhoeae isolates was used to evaluate and optimize the assay. the method employs two minor-groove binding prob ... | 2005 | 16272500 |
| characteristics of the m2000 automated sample preparation and multiplex real-time pcr system for detection of chlamydia trachomatis and neisseria gonorrhoeae. | we evaluated a new real-time pcr-based prototype assay for the detection of chlamydia trachomatis and neisseria gonorrhoeae developed by abbott molecular inc. this assay is designed to be performed on an abbott m2000 real-time instrument system, which consists of an m2000sp instrument for sample preparation and an m2000rt instrument for real-time pcr amplification and detection. the limit of detection of this prototype assay was determined to be 20 copies of target dna for both c. trachomatis an ... | 2007 | 17202273 |
| the unique branching patterns of deinococcus glycogen branching enzymes are determined by their n-terminal domains. | glycogen branching enzymes (gbe) or 1,4-alpha-glucan branching enzymes (ec 2.4.1.18) introduce alpha-1,6 branching points in alpha-glucans, e.g., glycogen. to identify structural features in gbes that determine their branching pattern specificity, the deinococcus geothermalis and deinococcus radiodurans gbe (gbe(dg) and gbe(dr), respectively) were characterized. compared to other gbes described to date, these deinococcus gbes display unique branching patterns, both transferring relatively short ... | 2009 | 19139240 |
| relationship between oral malodor and the global composition of indigenous bacterial populations in saliva. | oral malodor develops mostly from the metabolic activities of indigenous bacterial populations within the oral cavity, but whether healthy or oral malodor-related patterns of the global bacterial composition exist remains unclear. in this study, the bacterial compositions in the saliva of 240 subjects complaining of oral malodor were divided into groups based on terminal-restriction fragment length polymorphism (t-rflp) profiles using hierarchical cluster analysis, and the patterns of the microb ... | 2010 | 20228112 |
| effect of stoned olive pomace on rumen microbial communities and polyunsaturated fatty acid biohydrogenation: an in vitro study. | stoned olive pomace (sop), which represents approximately 50% of the conversion process of olives to olive oil, is largely not utilised and creates costs for its disposal and has negative environmental impacts. in vitro trial experiments were employed to study the effect of feeds integrated with this bio-waste, which is rich in polyphenols, on rumen biohydrogenation, using sheep rumen liquor as inoculum. | 2014 | 25424901 |