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studies of the cellular and free lipopolysaccharides form neisseria canis and n. subflava.cellular and free lipopolysaccharides (lps) obtained from neisseria canis and n. subflava were essentially identical. both cellular and free lipopolysaccharides contained o-polysaccharides of the following composition: l-rhamnose (46 mol), d-glucose (16 mol), l-glycero-d-manno-heptose (2 mol), ethanolamine (2 mol), 3-deoxy-d-manno-octulosonic acid (1 mol), and phosphate (1.5 mol). the core oligosaccharide, which was common to the cellular and free lps of both organisms, contained l-rhamnose (4 m ...19761260526
isolation of neisseria canis in mixed culture from a patient after a cat bite.we report a case of identification of neisseria canis in a human patient after a cat bite. this organism was isolated from a mixed culture together with pasteurella multocida and eikenella corrodens. it is the second case report of a human infection.19892768456
phylogeny of species in the family neisseriaceae isolated from human dental plaque and description of kingella oralis sp. nov [corrected].fourteen human periodontal isolates recovered from a purported eikenella corrodens-selective medium containing 1 microgram of clindamycin per ml displayed biochemical traits which differed from those described for e. corrodens. these organisms were gram-negative rods which corroded agar. the isolates were oxidase positive and urease, indole, and esculin negative. they differed from e. corrodens in catalase, nitrate reduction, lysine decarboxylase, and ornithine decarboxylase activities. one isol ...19938347509
neisseria weaveri sp. nov., formerly cdc group m-5, a gram-negative bacterium associated with dog bite wounds.cdc group m-5 is a rod-shaped, gram-negative, nonmotile bacterium associated with dog bite wounds. dna-dna relatedness and biochemical and growth characteristics were studied for 54 strains from the collection at the centers for disease control and prevention. one typical m-5 strain, 8142, was further studied by 16s rrna sequencing. dna from 40 of 53 strains showed 82 to 100% relatedness (hydroxyapatite method) to labeled dna from strain 8142. the guanine-plus-cytosine (g + c) content in 8 of th ...19938408570
bacterial community dynamics during start-up of a trickle-bed bioreactor degrading aromatic compounds.this study was performed with a laboratory-scale fixed-bed bioreactor degrading a mixture of aromatic compounds (solvesso100). the starter culture for the bioreactor was prepared in a fermentor with a wastewater sample of a care painting facility as the inoculum and solvesso100 as the sole carbon source. the bacterial community dynamics in the fermentor and the bioreactor were examined by a conventional isolation procedure and in situ hybridization with fluorescently labeled rrna-targeted oligon ...19989501433
structural and evolutionary inference from molecular variation in neisseria porins.the porin proteins of the pathogenic neisseria species, neisseria gonorrhoeae and neisseria meningitidis, are important as serotyping antigens, putative vaccine components, and for their proposed role in the intracellular colonization of humans. a three-dimensional structural homology model for neisseria porins was generated from escherichia coli porin structures and n. meningitidis pora and porb sequences. the neisseria sequences were readily assembled into the 16-strand beta-barrel fold charac ...199910225902
linezolid activity compared to those of selected macrolides and other agents against aerobic and anaerobic pathogens isolated from soft tissue bite infections in humans.linezolid was tested against 420 aerobes and anaerobes, including 148 pasteurella isolates, by an agar dilution method. linezolid was active against all pasteurella multocida subsp. multocida and p. multocida subsp. septica isolates and most pasteurella canis, pasteurella dagmatis, and pasteurella stomatis isolates. the mic was </=2 microg/ml for staphylococci, streptococci, ef-4b, weeksella zoohelcum, fusobacterium nucleatum, other fusobacteria, porphyromonas spp., prevotella spp., peptostrepto ...199910348773
activity of gatifloxacin compared to those of five other quinolones versus aerobic and anaerobic isolates from skin and soft tissue samples of human and animal bite wound infections.the activity of gatifloxacin against 308 aerobes and 112 anaerobes isolated from bite wound infections was studied. gatifloxacin was active at </=0.016 microg/ml against all 148 pasteurella isolates (eight species and three subspecies) tested and all other aerobes tested, including actinobacillus-haemophilus spp., eikenella corrodens, neisseria weaveri, weeksella zoohelcum, staphylococci, and streptococci. fusobacteria were sometimes resistant. gatifloxacin mics at which 90% of the isolates were ...199910348774
neisseria canis infection: a case report.the third case report, which is the first in australia, of human infection with neisseria canis is documented. this is the first case report in which the pathogenicity of this organism for humans is unequivocally demonstrated.199910497835
analysis of lipooligosaccharide biosynthesis in the neisseriaceae.neisserial lipooligosaccharide (los) contains three oligosaccharide chains, termed the alpha, beta, and gamma chains. we used southern hybridization experiments on dna isolated from various neisseria spp. to determine if strains considered to be nonpathogenic possessed dna sequences homologous with genes involved in the biosynthesis of these oligosaccharide chains. the presence or absence of specific genes was compared to the los profiles expressed by each strain, as characterized by their mobil ...200111208792
diagnostics of neisseriaceae and moraxellaceae by ribosomal dna sequencing: ribosomal differentiation of medical microorganisms.fast and reliable identification of microbial isolates is a fundamental goal of clinical microbiology. however, in the case of some fastidious gram-negative bacterial species, classical phenotype identification based on either metabolic, enzymatic, or serological methods is difficult, time-consuming, and/or inadequate. 16s or 23s ribosomal dna (rdna) bacterial sequencing will most often result in accurate speciation of isolates. therefore, the objective of this study was to find a hypervariable ...200111230407
long-term respiratory tract infection with canine-associated pasteurella dagmatis and neisseria canis in a patient with chronic bronchiectasis.pasteurella dagmatis and neisseria canis were repeatedly isolated from the sputum of a poodle-owning patient with chronic bronchiectasis. commercially available systems failed to identify these unusual organisms: identification was made by 16s rrna gene sequencing. difficulties identifying these and five other canine-associated isolates (p. dagmatis [n = 2], pasteurella canis [n = 2], and n. canis [n = 1]) are discussed.200516081998
cultivable oral microbiota of domestic dogs.bacteria were isolated from the dental plaques of nine dogs and a sample of pooled saliva from five other dogs and were then identified by comparative 16s rrna gene sequencing. among 339 isolates, 84 different phylotypes belonging to 37 genera were identified. approximately half of the phylotypes were identified to the species level, and 28% of these were considered members of the indigenous oral microbiota of humans. the 16s rrna gene sequences of the remaining 44 phylotypes were not represente ...200516272472
determination of the antimicrobial properties of oligo-2-hydroxy-1-naphthaldehyde.oligo-2-hydroxy-1-naphthaldehyde (ohna) was synthesized by oxidative polycondensation using h2o2 (35%, aqueous solution), air o2 and naocl (34%, aqueous solution) by kaya and senol and the products were characterized by spectral techniques. antimicrobial activities of the first and second fractions of ohna were tested against corynobacterium xerosis ccm 2824, proteus vulgaris atcc 6897, staphylococcus epidermidis nrrl b-4877, s. aureus atcc 6538, enterobacter aerogenes atcc 13048, salmonella thy ...200516355978
aggregative behavior of bacteria isolated from canine dental plaque.interbacterial adhesion of bacteria isolated from canine dental plaque was assessed by performing a visual coaggregation assay. using conditions mimicking those likely to be encountered in vivo, the entire cultivable plaque microbiota from a single dog was assessed, and eight (6.7%) unique coaggregation interactions were detected for 120 crosses. transmission electron microscopy was used to visualize several of the bacteria in isolation and as coaggregates, which revealed surface structures that ...200616885267
classification of centers for disease control group eugonic fermenter (ef)-4a and ef-4b as neisseria animaloris sp. nov. and neisseria zoodegmatis sp. nov., respectively.a polyphasic taxonomic study was performed on isolates classified as centers for disease control group eugonic fermenter (ef)-4a and ef-4b. comparative 16s rrna gene sequence analysis confirmed that group ef-4a and ef-4b belong to the genus neisseria with neisseria canis and neisseria dentiae as the nearest phylogenetic neighbours. dna-dna hybridizations and biochemical analyses demonstrated that isolates of group ef-4a and ef-4b represent two novel species within this sublineage of the genus ne ...200616902011
characteristics of the m2000 automated sample preparation and multiplex real-time pcr system for detection of chlamydia trachomatis and neisseria gonorrhoeae.we evaluated a new real-time pcr-based prototype assay for the detection of chlamydia trachomatis and neisseria gonorrhoeae developed by abbott molecular inc. this assay is designed to be performed on an abbott m2000 real-time instrument system, which consists of an m2000sp instrument for sample preparation and an m2000rt instrument for real-time pcr amplification and detection. the limit of detection of this prototype assay was determined to be 20 copies of target dna for both c. trachomatis an ...200717202273
management of human and animal bite wound infection: an overview.animal and human bite wounds can lead to serious infections. the organisms recovered generally originate from the biter's oral cavity and the victim's skin flora. anaerobes were isolated from more than two thirds of human and animal bite infections. streptococcus pyogenes is often recovered in human bites, pasteurella multocida in animal bites, eikenella corrodens in animal and human, capnocytophaga spp, neisseria weaveri, weeksella zoohelcum, neisseria canis, staphylococcus intermedius, nonoxid ...200919698283
neisseria wadsworthii sp. nov. and neisseria shayeganii sp. nov., isolated from clinical specimens.an analysis of 16s rrna gene sequences from archived clinical reference specimens has identified two novel neisseria species. for each species, two strains from independent sources were identified. amongst species with validly published names, the closest species to the newly identified organisms were neisseria canis, n. dentiae, n. zoodegmatis, n. animaloris and n. weaveri. dna-dna hybridization studies demonstrated that the newly identified isolates represent species that are distinct from the ...201120173010
first reported isolation of neisseria canis from a deep facial wound infection in a dog.neisseria canis was isolated in pure culture from a mandibular abscess in a dog. ultrasound-guided fine-needle aspiration was used to obtain a sample from the abscess. conventional bacteriological examination techniques followed by 16s rrna gene sequencing from pure subculture and construction of a phylogenetic tree verified the isolate as n. canis. 16s rrna sequence analysis revealed that a broader phylogenetic platform is needed in the part of the phylogenetic tree where the canine pathogenic ...201121411579
microbiology of animal bite wound infections.the microbiology of animal bite wound infections in humans is often polymicrobial, with a broad mixture of aerobic and anaerobic microorganisms. bacteria recovered from infected bite wounds are most often reflective of the oral flora of the biting animal, which can also be influenced by the microbiome of their ingested prey and other foods. bacteria may also originate from the victim's own skin or the physical environment at the time of injury. our review has focused on bite wound infections in ...201121482724
Identification of the antibacterial compound produced by the marine epiphytic bacterium Pseudovibrio sp. D323 and related sponge-associated bacteria.Surface-associated marine bacteria often produce secondary metabolites with antagonistic activities. In this study, tropodithietic acid (TDA) was identified to be responsible for the antibacterial activity of the marine epiphytic bacterium Pseudovibrio sp. D323 and related strains. Phenol was also produced by these bacteria but was not directly related to the antibacterial activity. TDA was shown to effectively inhibit a range of marine bacteria from various phylogenetic groups. However TDA-prod ...201121892353
subgingival microbiota in health compared to periodontitis and the influence of smoking.the etiology of periodontitis has traditionally been associated to a consortium of three bacterial species-the so-called "red-complex" of periodontal disease-which has been the target for most diagnostic and therapeutic strategies. however, other species have also been found to correlate with disease severity. in addition, the influence of smoking on periodontal microbiota is poorly understood. in the current manuscript, the composition of the subgingival microbiota in healthy individuals vs. pa ...201525814980
the use of pcr/electrospray ionization-time-of-flight-mass spectrometry (pcr/esi-tof-ms) to detect bacterial and fungal colonization in healthy military service members.the role of microbial colonization in disease is complex. novel molecular tools to detect colonization offer theoretical improvements over traditional methods. we evaluated pcr/electrospray ionization-time-of-flight-mass spectrometry (pcr/esi-tof-ms) as a screening tool to study colonization of healthy military service members.201627448413
marine mammals harbor unique microbiotas shaped by and yet distinct from the sea.marine mammals play crucial ecological roles in the oceans, but little is known about their microbiotas. here we study the bacterial communities in 337 samples from 5 body sites in 48 healthy dolphins and 18 healthy sea lions, as well as those of adjacent seawater and other hosts. the bacterial taxonomic compositions are distinct from those of other mammals, dietary fish and seawater, are highly diverse and vary according to body site and host species. dolphins harbour 30 bacterial phyla, with 2 ...201626839246
application of smartgene idns software to partial 16s rrna gene sequences for a diverse group of bacteria in a clinical laboratory.laboratories often receive clinical isolates for bacterial identification that have ambiguous biochemical profiles by conventional testing. with the emergence of 16s rrna gene sequencing as an identification tool, we evaluated the usefulness of smartgene idns, a 16s rrna sequence database and software program for microbial identification. identification by conventional methods of a diverse group of bacterial clinical isolates was compared with gene sequences interrogated by the smartgene and mic ...200617050811
a systematic approach for discovering novel, clinically relevant bacteria.sequencing of the 16s rrna gene (16s) is a reference method for bacterial identification. its expanded use has led to increased recognition of novel bacterial species. in most clinical laboratories, novel species are infrequently encountered, and their pathogenic potential is often difficult to assess. we reviewed partial 16s sequences from >26,000 clinical isolates, analyzed during february 2006-june 2010, and identified 673 that have <99% sequence identity with valid reference sequences and ar ...201222377371
culturable gut microbiota diversity in zebrafish.the zebrafish (danio rerio) is an increasingly used laboratory animal model in basic biology and biomedicine, novel drug development, and toxicology. the wide use has increased the demand for optimized husbandry protocols to ensure animal health care and welfare. the knowledge about the correlation between culturable zebrafish intestinal microbiota and health in relation to environmental factors and management procedures is very limited. a semi-quantitative level of growth of individual types of ...201222428747
a wide spectrum of fastidious and ampicillin-susceptible bacteria dominate in animal-caused wounds.the main purpose of this study was to assess the actual occurrence of gram-negative oxidase-positive bacteria (gnop) in human wounds caused by animals, mostly cat and dog bites and scratches, and with signs of infection. we report a prospective series of 92 wound samples. routine culturing was combined with a procedure optimised for fastidious gnop. all gnop isolates were identified by 16s rdna sequencing to the species level. we observed a more prominent role of gnop, including at least 30 spec ...201627197725
improved method for polynucleotide probe-based cell sorting, using dna-coated microplates.we developed an improved method for cultivation-independent sorting of bacterial cells. the technique is based on labeling the target cells by in situ hybridization with polynucleotide transcript probes. due to the probes' length, part of the probe remains outside the cell and can subsequently be used to capture the cells. target cells are immobilized during a second hybridization step in microplates that are coated with dna that is complementary to the probe sequence. the method was applied suc ...200414711680
a genomic approach to bacterial taxonomy: an examination and proposed reclassification of species within the genus neisseria.in common with other bacterial taxa, members of the genus neisseria are classified using a range of phenotypic and biochemical approaches, which are not entirely satisfactory in assigning isolates to species groups. recently, there has been increasing interest in using nucleotide sequences for bacterial typing and taxonomy, but to date, no broadly accepted alternative to conventional methods is available. here, the taxonomic relationships of 55 representative members of the genus neisseria have ...201222422752
identifying neisseria species by use of the 50s ribosomal protein l6 (rplf) gene.the comparison of 16s rrna gene sequences is widely used to differentiate bacteria; however, this gene can lack resolution among closely related but distinct members of the same genus. this is a problem in clinical situations in those genera, such as neisseria, where some species are associated with disease while others are not. here, we identified and validated an alternative genetic target common to all neisseria species which can be readily sequenced to provide an assay that rapidly and accur ...201424523465
genome sequence analyses show that neisseria oralis is the same species as 'neisseria mucosa var. heidelbergensis'.phylogenies generated from whole genome sequence (wgs) data provide definitive means of bacterial isolate characterization for typing and taxonomy. the species status of strains recently defined with conventional taxonomic approaches as representing neisseria oralis was examined by the analysis of sequences derived from wgs data, specifically: (i) 53 neisseria ribosomal protein subunit (rps) genes (ribosomal multi-locus sequence typing, rmlst); and (ii) 246 neisseria core genes (core genome mlst ...201324097834
draft genome sequence of a taxonomically unique neisseria strain isolated from a greater white-fronted goose (anser albifrons) egg on the north slope of alaska.we report here the draft genome sequence of a unique neisseria strain that was isolated from a greater white-fronted goose (anser albifrons) egg. the sequencing was performed with an illumina miseq system, and the sequence consists of 275 contigs. the total genome is 2,397,978 bp long and has a g+c content of 46.4%.201526184936
assessing the effectiveness of functional genetic screens for the identification of bioactive metabolites.a common limitation for the identification of novel activities from functional (meta) genomic screens is the low number of active clones detected relative to the number of clones screened. here we demonstrate that constructing libraries with strains known to produce bioactives can greatly enhance the screening efficiency, by increasing the "hit-rate" and unmasking multiple activities from the same bacterial source.201223271424
assessing the effectiveness of functional genetic screens for the identification of bioactive metabolites.a common limitation for the identification of novel activities from functional (meta) genomic screens is the low number of active clones detected relative to the number of clones screened. here we demonstrate that constructing libraries with strains known to produce bioactives can greatly enhance the screening efficiency, by increasing the "hit-rate" and unmasking multiple activities from the same bacterial source.201223271424
a cross-sectional survey of bacterial species in plaque from client owned dogs with healthy gingiva, gingivitis or mild periodontitis.periodontal disease is the most widespread oral disease in dogs which if left untreated results in significant pain to the pet and loss of dentition. the objective of this study was to identify bacterial species in canine plaque that are significantly associated with health, gingivitis and mild periodontitis (<25% attachment loss). in this survey subgingival plaque samples were collected from 223 dogs with healthy gingiva, gingivitis and mild periodontitis with 72 to 77 samples per health status ...201324349448
microbial infections are associated with embryo mortality in arctic-nesting geese.to address the role of bacterial infection in hatching failure of wild geese, we monitored embryo development in a breeding population of greater white-fronted geese (anser albifrons) on the arctic coastal plain of alaska. during 2013, we observed mortality of normally developing embryos and collected 36 addled eggs for analysis. we also collected 17 infertile eggs for comparison. using standard culture methods and gene sequencing to identify bacteria within collected eggs, we identified a poten ...201526048928
first draft genome sequences of neisseria sp. strain 83e34 and neisseria sp. strain 74a18, previously identified as cdc eugonic fermenter 4b species.we report the first draft genome sequences of two isolates previously classified as cdc ef-4b species, neisseria sp. 83e34 and neisseria sp. 74a18. both strains were isolated from patients with animal bites and likely constitute novel genomospecies with average nucleotide identities of <95% to other sequenced strains.201627834718
zoonotic streptococcus canis infection in singapore. 201627076048
isolation and characterization of a neisseria strain from the liver of a chinese peking duck.a neisseria strain, neisseria sp. ah-n10, was isolated from liver of a chinese peking duck and characterized using a number of phenotypic and genotypic approaches. based on scanning electron microscopy examination, the isolated strain has the typical structure of neisseria species. sequence comparison of 16s rrna gene and phylogenetic analysis suggest that neisseria sp. ah-n10 is closely related to neisseria canis, which was previously isolated from a human dog bite wound. animal infection exper ...201424681266
neisseria arctica sp. nov. isolated from nonviable eggs of greater white-fronted geese (anser albifrons) in arctic alaska.during the summers of 2013 and 2014, isolates of a novel gram-negative coccus in the neisseria genus were obtained from the contents of nonviable greater white-fronted goose (anser albifrons) eggs on the arctic coastal plain of alaska. we used a polyphasic approach to determine whether these isolates represent a novel species. 16s rrna gene sequences, 23s rrna gene sequences, and chaperonin 60 gene sequences suggested that these alaskan isolates are members of a distinct species that is most clo ...201728056218
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