| the value of epicillin and ampicillin in the treatment of meningococcal and pneumococcal meningitis. | 96 patients with meningitis due to neisseria meningitidis and diplococcus pneumoniae were treated with epicillin or ampicillin according to a predesigned randomization chart. results indicate that epicillin and ampicillin are effective single drugs in the treatment of meningococcal and pneumococcal meningitis. no adverse reactions were noted with either drug and they were comparable in their efficacy. | 1976 | 9689 |
| acute bacterial meningitis at boston city hospital during 12 selected years, 1935-1972. | data are presented on the occurrence of and mortality rate from acute bacterial meningitis at boston city hospital during 12 years between 1935 and 1972 selected in relation to the introduction of potent antibacterial agents. the most frequent causative organisms were streptococcus pneumoniae, neisseria meningitidis, and haemophilus influenzae, but large proportions were caused by other gram-positive cocci and gram-negative bacilli. the greatest reduction in mortality rate after antibiotics beca ... | 1977 | 20479 |
| evaluation of the counterimmunoelectrophoretic (cie) procedure in a clinical laboratory setting. | the counterimmunoelectrophoretic (cie) procedure was evaluated under clinical laboratory conditions to determine its validity and comparability with culture methods. the procedure was further evaluated to determine applicability to a variety of clinical samples. an inexpensive set-up was developed to utilize the cie procedure at bench level. results indicated the procedure to be sensitive in detecting haemophilus influenzae, type b, and neisseria meningitidis (meningococcus), group b. the proced ... | 1978 | 29483 |
| serological methods for rapid diagnosis of haemophilus influenzae, neisseria meningitidis and streptococcus pneumoniae in cerebrospinal fluid: a comparison of co-agglutination, immunofluorescence and immunoelectroosmophoresis. | | 1978 | 31678 |
| prospects for the prevention of bacterial meningitis with polysaccharide vaccines. | most suppurative infections of the meninges are caused by five bacterial species: escherichia coli, haemophilus influenzae type b, streptococcus pneumoniae, neisseria meningitidis, and group b streptococcus. the immune response of adults to pneumococcal capsular polysaccharides has been studied in great detail and their responses to meningococcal and h. influenzae type b capsular polysaccharides are quite similar. immune responses of adults to e. coli and group b streptococcal antigens are disap ... | 1978 | 31985 |
| effect of ampicillin and chloramphenicol against streptococcus pneumoniae and neisseria meningitidis. | antagonism, determined by isobolograms constructed from data from combinations of ampicillin and chloramphenicol at or below the minimal inhibitory or bactericidal concentrations, was observed against 13 clinical isolates of meningococcus and against one isolate of pneumococcus. synergy occurred against six strains of pneumococcus and three of meningococcus. additive effects were noted against 14 isolates of pneumococcus and 5 of meningococcus. there was no relationship between the minimal inhib ... | 1979 | 34360 |
| comparison of staphylococcal coagglutination, latex agglutination, and counterimmunoelectrophoresis for bacterial antigen detection. | soluble antigens of haemophilus influenzae type b, streptococcus pneumoniae, neisseria meningitidis, and group b streptococcus were looked for in cerebrospinal fluid, serum, and urine by using the staphylococcal coagglutination test, latex agglutination test, and counterimmunoelectrophoresis. the staphylococcal coaggultination and latex agglutination tests were more sensitive than counterimmunoelectrophoresis in identifying antigens of h. influenzae type b, s. pneumoniae, and n. meningitidis. no ... | 1979 | 34622 |
| bactericidal and bacteriostatic action of chloramphenicol against memingeal pathogens. | the bacteriostatic and bactericidal effects of chloramphenicol, ampicillin, tetracycline, and sulfisoxazole were compared against several potential meningeal pathogens. chloramphenicol is bactericidal at clinically achievable concentrations against haemophilus influenzae, streptococcus pneumoniae, and neisseria meningitidis. it is bacteriostatic against gram-negative bacilli of the family enterobacteriaceae and against staphylococcus aureus. chloramphenicol has proven highly efficacious in the t ... | 1979 | 38742 |
| pathogenic species of the genus haemophilus and streptococcus pneumoniae produce immunoglobulin a1 protease. | thirty-seven strains of the genus haemophilus and five strains of streptococcus pneumoniae were examined for their ability to produce extracellular enzyme that cleaves immunoglobulin molecules. all strains of h. influenzae, h. aegyptius, and s. pneumoniae elaborated enzyme that selectively cleaved human immunoglobulin a1 (iga1) myeloma proteins but was inactive against a variety of other proteins including human iga2, igg, and igm, porcine and bovine secretory iga, human and bovine serum albumin ... | 1979 | 40878 |
| rosamicin: in vitro activity comparison with erythromycin and other antibiotics against clinical isolates from the genito-urinary tract and neisseria meningitidis. | | 1979 | 41833 |
| opsonic activity of normal human cerebrospinal fluid for selected bacterial species. | the opsonic activity of normal human cerebrospinal fluid (csf) has not been well defined. in this study, the opsonic activity of normal csf for laboratory and blood culture isolates of staphylococcus aureus, streptococcus pneumoniae, escherichia coli, hemophilus influenzae type b, and neisseria meningitidis was measured by a quantitative assay employing radiolabeled bacteria and polymorphonuclear leukocytes. all isolates of s. aureus, except the wood 46 strain, were opsonized in undiluted csf (> ... | 1979 | 43289 |
| emergence of antibiotic resistance in hospitals, 1935-1975. | a limited review of the changes in susceptibility of common bacterial pathogens to available antibacterial agents is presented. significant developments in recent years include the following: (1) the emergence of streptococcus pneumoniae with decreased resistance to penicillin and of some strains resistant to several antibiotics; (2) a decline in prevalence of multi-drug-resistant staphylococcus aureus after 1960 following their increasing prevalence in the preceding years (these changes were me ... | 1979 | 45521 |
| fulminant meningococcal septicaemia. a hospital experience. | in the first 5 months of 1975, 12 cases of meningococcal septicaemia were seen at a children's hospital where in previous years the condition was seen only sporadically. 3 of these children were dead on admission, 5 died shortly after admission, and 4 responded to treatment. neisseria meningitidis was recovered from cerebrospinal fluid or blood or both in all cases, and the clinical illness was characterised by fever, prostration, and cutaneous purpura of abrupt onset. there were no significant ... | 1975 | 49707 |
| [cultivation of neisseria meningitidis serogroup a on semisynthetic liquid nutrient media]. | in cultivation of meningococcus of serological group a in fluid semisynthetic medium of simple composition prepared on the basis of purified acid casein hydrolysate with profound splitting there were obtained microbial cultures with a density of 4-5 x 10(9) microbial cells per 1 ml after 20-24 hours of cultivation with shaking. alkalinity of the medium increased (to ph 8.0-8.2 during the stationary phase) with increase of the microbial cell concentration. a study of the accumulation of group-spe ... | 1975 | 53974 |
| response to antigenic determinants of neisseria meningitidis lipopolysaccharide investigated with a new radioactive antigen-binding assay. | adaptations of the farr technique have resulted in a specific and reproducible radioactive antigen-binding assay for antibodies directed against the lipopolysaccharide (lps) of neisseria meningitidis. lps was intrinsically labeled with 14c acetate during 16-hr growth in a modified frantz media, extracted by hot phenol-h2o, and purified by dialysis, ultracentrifugation, and ethanol precipitation. lps, which aggregates in aqueous solutions, was maintained in a monomeric form in 3% sodium deoxychol ... | 1976 | 56401 |
| the relationship between neisseria meningitidis of serogroups z1 and 29e. | meningococci of serogroups z1 and 29e were examined serologically and shown to be identical. these meningococci should be designated either as group z1, which has priority, or preferably by a new designation forming a logical sequence with the currently accepted serogroups. | 1976 | 58994 |
| a study of the protein-polysaccharide complex of neisseria meningitidis serogroup a. | | 1976 | 62709 |
| simultaneous infection of the meninges by coxsackie b virus and neisseria meningitidis in an elderly female. | | 1976 | 63876 |
| cross-antigenicity and immunogenicity between capsular polysaccharides of group c neisseria meningitidis and of escherichia coli k92. | antibodies to capsular polysaccharides of group c neisseria meningitidis are often found in sera of young adults despite infrequent nasopharyngeal carriage and low rate of attack of n. meningitidis in the united states. thus, experiments were designed for detection of bacteria cross-reactive with n. meningitidis. among 3,264 cultures of stool, urine, blood, and cerebrospinal fluid, only 14 strains were found to be cross-reactive; all were escherichia coli possessing the k92 capsular polysacchari ... | 1977 | 64575 |
| counterimmunoelectrophoresis in the diagnosis of bacterial meningitis. | the aim of the present study was to investigate whether counterimmunoelectrophoresis (cie) would facilitate the rapid, etiological diagnosis of bacterial meningitis when used in parallel with other routine methods in a medical bacteriological laboratory. of 3,674 consecutive specimens of cerebrospinal fluid (csf) received at the department of diagnostic bacteriology, statens seruminstitu, 283 specimens (each representing one patient) were selected for examination by cie on the basis of the follo ... | 1977 | 67124 |
| isolation of neisseria meningitidis in urogenital/rectal infections. | in 9 cases (6 men, 3 women) during 1975 we have isolated n. meningitidis from routine samples from urogenital/rectal locations to be examined for gonococci by culture. one of the men harboured meningococci in the rectum, the other 5 in the urethra. two of the women had meningococci in the cervix and one in the urethra. of the meningococci isolated, one belonged to serogroup a, 6 to group b and 2 to group c. six of the patients, 4 men and 2 women, who attended the department of venereal diseases, ... | 1977 | 71815 |
| outer-membrane protein and lipopolysaccharide serotyping of neisseria meningitidis by inhibition of a solid-phase radioimmunoassay. | a new procedure involving inhibition of a solid-phase radioimmunoassay was developed for specific determination of the outer-membrane protein and the lipopolysaccharide (lps) serotypes of meningococci. antigen was allowed to bind to the wells of a polyvinyl microtiter plate and then reacted with a limiting amount of homologous antibody which had been preincubated with buffer or a standard concentration of inhibiting antigen. the amount of antibody bound per well was quantitated by incubation wit ... | 1977 | 72735 |
| [antigenic characteristics and various biological properties of glycoprotein from neisseria meningitidis of serological group a]. | it was shown that the antigen determining the group specificity of meningococcus belonging to serological group a was of mixed polysaccharide-protein nature. carbohydrate component is responsible for the interaction with the group-specific antibodies in this antigen. glycoprotein can be isolated both from the cells and from the culture fluid where it passes during the n. meningitidis cultivation in fluid nutrient medium. the described antigen possesses no properties of endotoxin. | 1978 | 75626 |
| safety and immunogenicity of a neisseria meningitidis type 2 protein vaccine in animals and humans. | two neisseria meningitidis vaccines consisting principally of outer membrane protein (lot 138i-0) or outer membrane protein plus group c polysaccharide (lot 138i-m1) were prepared from the group c type 2 strain 138i. lipopolysaccharide and lipid were removed by gel filtration in the presence of sodium deoxycholate. the vaccines were found to be nontoxic and nonpyrogenic in animals. they provided active protection in mice against mucin-enhanced killing by group b type 2 meningococci and induced g ... | 1978 | 77885 |
| is group-specific meningococcal vaccination resulting in epidemics caused by groups of virulent meningococci? | in 1976 routine vaccination against neisseria meningitidis serogroups a and c was started in the finnish armed forces. a case of fulminant, complicated pneumonia caused by group-y meningococcus in a vaccinated recruit, prompted a study of the distribution of the meningococcal groups isolated from the recruits in the same unit. 14 (46%) of the 31 isolates from 84 recruits were group y. group-y meningococcus was rarely isolated from unvaccinated controls. these results suggest that widespread vacc ... | 1978 | 79765 |
| crossed immunoelectrophoretic analysis of neisseria meningitidis antigens and of corresponding antibodies in patients with meningococcal disease. | sixty-three different antigens of neisseria meningitidis groups a, b and c were demonstrated by means of crossed immunoelectrophoresis against rabbit antiserum. the group-specific polysaccharides a and c were identified in the immunoprecipitate pattern. the majority of the remaining antigens were common to all three groups of meningococci. a clearcut rise in antibodies was found in all of 27 patients with meningococcal meningitis or septicaemia. these antibodies were directed against both group- ... | 1978 | 81590 |
| precipitating antibodies against neisseria meningitidis in normal sera and their possible origin. | crossed immunoelectrophoresis was used to study precipitating antibodies against neisseria meningitidis in sera from 214 normal persons and in human gammaglobulin. a polyspecific meningococcal antigen preparation and a corresponding rabbit antiserum constituted the reference system. all the sera contained one to five precipitins against n. meningitidis which could be identified and quantified by means of the reference system. the precipitin score, which expresses the number and titre of precipit ... | 1978 | 82371 |
| comparison of counter-current immunoelectrophoresis, latex agglutination, and radioimmunoassay in detection of soluble capsular polysaccharide antigens of haemophilus influenzae type b and neisseria meningitidis of groups a or c. | three serological methods, radioimmunoassay (ria), latex agglutination (lx), and counter-current immunoelectrophoresis (ciep), for sensitivity in the detection of the capsular polysaccharide antigen of haemophilus influenzae type b or neisseria meningitidis groups a and c were compared. ria was consistently the most sensitive, lx the next, and ciep the least sensitive. when ria and lx were used to test cerebrospinal fluid (csf) samples of patients with meningitis, they gave very similar results. ... | 1978 | 85636 |
| lipopolysaccharide-derived serotype polysaccharides from neisseria meningitidis group b. | three immunologically distinct types of polysaccharides have been isolated by diethylaminoethyl (deae) chromatography from the lipopolysaccharide extracts of group b neisseria meningitidis. all types contain a set of common determinants, as well as distinct ones; all of these determinants are detectable by either immunodiffusion or enzyme-linked immunosorbent assay (elisa). the polysaccharides elute from a sepharose 4b column in the range of 2-3 x 10(5) daltons and have isoelectric points from 4 ... | 1979 | 88491 |
| relative opsonic and protective activities of antibodies against k1, o and lipid a antigens of escherichia coli. | the k1 escherichia coli capsular antigen has been implicated as a virulence factor because of the frequency of isolation of k1 containing strains from certain invasive human infections. in the study of the interaction between k1 strains, normal human polymorphonuclear cells (pmns) and fresh human serum, we have found varying susceptibility to phagocytosis and killing; thus, the in vitro opsonophagocytic and in vivo protective role of k1, somatic o and core glycolipid antibodies remain unclear. w ... | 1979 | 93778 |
| lectins in diagnostic microbiology: use of wheat germ agglutinin for laboratory identification of neisseria gonorrhoeae. | a lectin slide agglutination test has been developed for the confirmatory identification of neisseria gonorrhoeae. with wheat germ lectin as an agglutinin, 164 of 165 clinical isolates of n. gonorrhoeae gave a 3 to 4+ reaction within 6 to 8 min. four gonococcal isolates, even though negative by the fluoresecent-antibody method, gave strong positive reactions with the wheat germ lectin. among 23 isolates of neisseria meningitidis tested, which included representatives of sero-groups a, b, c,d, x, ... | 1979 | 94598 |
| serogroup identification of neisseria meningitidis: comparison of an antiserum agar method with bacterial slide agglutination. | a serum agar method for serogrouping neisseria meningitidis is described and compared with conventional bacterial slide agglutination. there was 93% agreement for 300 strains examined individually by each method. among strains from serogroups a, b, c, y, and w135, there was 100% correlation, whereas strains from serogroup 29e (z') had only 67% correlation. the serum agar method was rapid, as well as easy to perform and interpret. the potential benefits of this method for epidemiological studies ... | 1978 | 96123 |
| identification of some basic extractable compounds produced by neisseria gonorrhoeae and neisseria meningitidis in a defined medium. | electron capture gas-liquid chromatography and mass spectrometry have been used to identify some of the basic extractable heptafluorobutyric anhydride reactive compounds found in a defined medium after 20 h of growth by neisseria gonorrhoeae and n. meningitidis. acetoin, 2,3-butanediol, pyrroline, and 1,3-diaminopropane were identified by both gas chromatography and mass spectrometry; 2-hydroxy-pyrrolidine and 3-aminomethyl-pyrrolidine were tentatively identified by mass spectrometry. a possible ... | 1978 | 96124 |
| identification and grouping of neisseria meningitidis directly on agar plates by coagglutination with specific antibody-coated protein a-containing staphylococci. | it has been shown that neisseria meningitidis can be grouped by coagglutination directly upon growth on sheep blood or chocolate agar plates. all positive reactions were group specific, and only a single colony was required for a positive reaction. there was variation seen in the effectiveness of commercial antisera in preparing sensitive reagents. certain throat and sputum isolates of group z organisms failed to react by coagglutination, although they would react directly with antiserum in whol ... | 1978 | 96128 |
| meningitis caused by neisseria meningitidis type w-135. | | 1978 | 97374 |
| effects of various divalent cations on the survival of neisseria gonorrhoeae in liquid media. | the effect of various concentrations of divalent cations on survival of gonococci in liquid medium was studied. the growth of neisseria gonorrhoeae was inhibited by manganous chloride in concentrations above 1 x 10(-5)mol/l while the growth of control organisms such as neisseria meningitidis and escherichia coli was not inhibited by the same salt even at 1 x 10(-2)mol/l. copper sulphate, cobaltous nitrate, nickel chloride, and zinc sulphate also had deleterious effects on gonococci. magnesium ch ... | 1978 | 98204 |
| outer membrane protein antigens in an enzyme-linked immunosorbent assay for salmonella enteric fever and meningococcal meningitis. | outer membrane protein preparations were obtained from strains of salmonella and neisseria meningitidis. solubilized cell envelope (ce) fractions from s. typhi and salmonella groups a, b, c, and e had very similar electrophoretic mobilities on polyacrylamide gel, and common antigens were demonstrated by immunodiffusion. ce appeared to be a more satisfactory antigen than the more purified preparation (t/tei) in the enzyme-linked immunosorbent assay (elisa) with sera from typhoid and paratyphoid p ... | 1978 | 99452 |
| piliation and colonial morphology among laboratory strains of meningococci. | colonial morphology and piliation were studied on twelve strains from various serogroups of neisseria meningitidis. six different colony types (m1 to m6) were identified. most strains elaborated only an m1 colonial type, which is similar to gonococcus t4. several combinations of piliation and colonial morphology were observed: (i) colonial variation in which neither parent nor variant were piliated; (ii) colonial variation involving piliated and nonpiliated cells; (iii) dissociation of piliated ... | 1978 | 99453 |
| [allergization of the body by neisseria meningitidis. i. the problem of the occurrence of n. meningitidis in cases of bronchial asthma of bacterial etiology (author's transl)]. | the object of the authors work was the examination of nasopharyngeal swabs on the prevalence of neisseria meningitidis. a group of 222 patients suffering from asthma bronchiale of bacteriological etiology was under observation in order to be able to prepare an autovaccine. nasopharyngeal swabs were inoculated on special nutrient media. neisseria meningitidis was found in 22%, mainly from nasopharyngeal swabs, together with haemophilus sp. and streptococcus pneumoniae. in 59% of examined cases th ... | 1978 | 99912 |
| cross-reactions between neisseria meningitidis and twenty-seven other bacterial species. | cross-reactions between antigens from n. meningitidis and antigens from 27 other bacterial species from clinical isolates representing 20 different genera were studied by quantitative immunoelectrophoretic methods. a water-soluble n. meningitidis antigen preparation and a corresponding pooled rabbit antiserum regularly presenting 48 immunoprecipitates were used as reference system. antigens from 16 other bacterial genera cross-reacted with one to four n. meningitidis antigens, whereas antigens f ... | 1978 | 99974 |
| iron in neisseria meningitidis: minimum requirements, effects of limitation, and characteristics of uptake. | a simple defined medium (neisseria defined medium) was devised that does not require iron extraction to produce iron-limited growth of neisseria meningitidis (sdic). comparison of this medium to mueller-hinton broth and agar showed nearly identical growth rates and yields. the defined medium was used in batch cultures to determine the disappearance of iron from the medium and its uptake by cells. to avoid a number of problems inherent in batch culture, continuous culture, in which iron and disso ... | 1978 | 101516 |
| a simple manganous chloride and congo red disc method for differentiating neisseria gonorrhoeae from neisseria meningitidis. | manganous chloride and congo red incorporated into blotting paper discs have been used to differentiate gonococci from meningococci. the new technique is simple and reliable; the materials for the test are inexpensive. the method will increase the efficiency of distinguishing between the pathogenic neisseria in any clinical bacteriology laboratory and especially in those in the tropical areas. | 1978 | 101555 |
| acute joint infection with neisseria meningitidis: a case of mistaken identity. | | 1978 | 101903 |
| liberation of endotoxin during growth of neisseria meningitidis in a chemically-defined medium. | modified medium 199 is well suited to the growth of meningococci if rich growth is not necessary. the bacteria were very sensitive to changes in ph and needed a good buffer capacity of the medium. four strains of neisseria meningitidis were studied. after the stationary phase had been reached, the vital cell count decreased relatively slowly. however, the total cell count remained unchanged for at least 12 hours. the standardized e. coli endotoxin could be detected easily by the limulus lysate t ... | 1978 | 102109 |
| heat-modifiable outer membrane proteins of neisseria meningitidis and their organization within the membrane. | neisseria meningitidis group b serotype 2 strain m986 contains two predominant outer membrane proteins, with apparent molecular weights of 41,000 (protein b) and 28,000 (protein e). heating of outer membrane vesicles at 56 degrees c for 20 min caused much of b** to disaggregate and denature into b (41,000 daltons). in contrast, protein e could be rapidly solubilized by sds at room temperature into its monomeric state (e*), but it was not converted to its final higher apparent molecular weight of ... | 1978 | 102633 |
| laboratory identification of pathogenic neisseria with special regard to atypical strains: an evaluation of sugar degradation, immunofluorescence and co-agglutination tests. | sugar degradation tests (sdt) were compared with immunofluorescence (ifl) and co-agglutination (coa) tests for the diagnosis of neisseria gonorrhoeae (gc) and neisseria meningitidis (mc). somewhat more than 5% of the gc strains and 8% of the mc strains were misinterpreted by sdt. on most occasions the disagreement between sdt and serological tests was due to the inability of the mc strains (less so for gc strains) to degrade sugars correctly. because of this, three out of 15 strains (20%) from p ... | 1978 | 103365 |
| meningitis caused by neisseria meningitidis serogroup 135. | the first descriptions of meningitis in childhood caused by neisseria meningitidis serogroup 135 are presented. difficulties with identification of unusual serogroups of n. meningitidis are discussed. | 1978 | 103892 |
| antibody responses of human infants to three doses of group a neisseria meningitidis polysaccharide vaccine administered at two, four, and six months of age. | infants were immunized with group a neisseria meningitidis polysaccharide vaccine at two, four, and six months of age. two lots of group a vaccine that differed in molecular weight were used; lot no. 1980a was of significantly larger molecular size than lot no. a-7. no significant adverse reactions occurred. the geometric mean concentration of serum antibody to group a n. meningitidis one month after the third dose of lot no. 1980a was 0.89 microgram/ml, nearly twice the concentration induced by ... | 1978 | 105053 |
| enzyme-linked immunosorbent assays for the detection of neisseria gonorrhoeae specific antibodies. | an indirect enzyme-linked immunosorbent assay (elisa) using rigid polystyrene microtiter plates was adapted to detect specific gonococcal antibodies against outer membrane-complex antigens extracted from neisseria gonorrhoeae. the concentration of antigen to obtain maximum coating of the well was 10 micrograms protein per millilitre. the optimal binding of the primary antibody and enzyme-conjugated antimmunoglobulin was achieved after 1 h at 37 degrees c. under these conditions using gonococcal ... | 1978 | 105793 |
| [antibioprophylaxis of meningococcal meningitis: reality or utopia? (author's transl)]. | after restating the activity of the various antibiotics on neisseria meningitidis, the authors review the results of 18 experiences of germ-carriers control by antibiotics, which were rather deceiving. conditions for such a control are exposed but question of its real necessity is raised. | 1978 | 106213 |
| raised intracranial pressure: its management in neisseria meningitidis meningoencephalitis. | further reduction in morbidity and mortality from bacterial meningitis is unlikely to stem from innovations in antibiotic management. emphasis must therefore be directed toward prevention, more rapid diagnosis, and improved therapy for maintaining the integrity of the cns. raised intracranial pressure is frequently associated with bacterial meningitis. in selected patients with bacterial meningitis and clinical evidence of elevated intracranial pressure, monitoring and aggressive treatment of th ... | 1979 | 106721 |
| [detection of healthy carriers of neisseria meningitidis in a sample of the population of the city of messina]. | | 1977 | 106778 |
| [presence of anti-neisseria meningitidis antibodies in the normal population of messina]. | | 1977 | 106779 |
| pharyngeal colonisation by neisseria gonorrhoeae and neisseria meningitidis in black and white patients attending a venereal disease clinic. | pharyngeal colonisation by neisseria gonorrhoeae and neisseria meningitidis was studies in 2000 patients attending a venereal disease clinic. of these patients, 64% were white and 36% were black. the incidence of gonococcal infections was highest in the period from june to august. the incidence of genital or rectal infections or both was higher in the black patients. pharyngeal colonisation by gonococci was present in 1.3% of the patients. there was no significant associations between pharyngeal ... | 1979 | 106918 |
| inhibition of neisseria gonorrhoeae by normal human saliva. | saliva was found to be a powerful and specific inhibitor of neisseria gonorrhoeae. although 28 other species of bacteria were tested, including neisseria meningitidis, neisseria pharyngis var flava, neisseria lactamica, and neisseria catarrhalis, we failed to find any others sensitive to saliva under similar conditions. the physical properties of the inhibitory substance indicated that it might be salivary alpha-amylase. to test this hypothesis alpha-amylase was extracted from saliva and was sho ... | 1979 | 106919 |
| [effects of chemotherapeutic agents on strains of neisseria meningitidis isolated in bohemia during 1970-1976]. | | 1979 | 106970 |
| electron capture gas-liquid chromatographic-mass spectral identification of acids produced by neisseria meningitidis in a defined medium. | the acid metabolites and the cellular fatty acids of three strains of neisseria meningitidis grown in a chemically defined liquid medium were determined with computerized frequency-pulsed electron capture gas-liquid chromatography. five acids not previously reported were subsequently identified: isobutyric, octanoic, decenoic (c10:1), dodecenoic (c12:1), and tetradecenoic (c14:1). these acids were produced during active metabolism and were not detected as cellular constituents. the frequency-pul ... | 1979 | 107187 |
| serotypes of neisseria meningitidis isolated from patients in norway during the first six months of 1978. | during the first 6 months of 1978, 114 strains of neisseria meningitidis isolated from patients in norway were serotyped. among 27 group c strains, type 2 was most common, whereas 82% of the 82 group b isolates did not react with antisera to the standard serotypes 1 to 12. these strains were shown to belong to a new serotype, type 15. also some group a and c strains had the type 15 antigen. investigations on a possible immunoprophylaxis against group b meningococcal disease in norway should acco ... | 1979 | 107188 |
| transport of neisseria meningitidis cultures: growth-supporting media and freezing as an alternative. | the abilities of transgrow (tg), thayer-martin (tm), and new york city (nyc) solid media to maintain the viability of 12 strains of neisseria meningitidis under various controlled conditions were assessed. the effects of charcoal impregnation of swabs, temperature, and an enriched co2 atmosphere were examined with holding for up to 21 days. recovery from samples held at 35 degrees c was, in almost all instances, greater than at 22 or 4 degrees c. a strong requirement for added co2 was demonstrat ... | 1979 | 108008 |
| metabolism of pyrimidine bases and nucleosides in neisseria meningitidis. | in neisseria meningitidis, uridine, deoxyuridine, cytosine, cytidine, or deoxycytidine could not be used by uracil-requiring mutants as pyrimidine sources. consistent with these findings, only 5-fluorouracil of the different fluoropyrimidine bases and nucleosides showed any inhibitory effect on the growth of four prototrophic strains of n. meningitidis. likewise, only radioactive uracil was readily incorporated into nucleic acids, whereas uptake of radioactive uridine, cytosine, or cytidine coul ... | 1979 | 108255 |
| comparative immunogenicity of vaccines prepared from capsular polysaccharides of group c neisseria meningitidis o-acetyl-positive and o-acetyl-negative variants and escherichia coli k92 in adult volunteers. | three structurally and antigenically similar capsular polysaccharides, two derived from group c neisseria meningitidis (o-acetyl-positive and o-acetyl-negative variants) and one from escherichia coli k92, which cross-reacts with polysaccharide from group c n. meningitidis, were compared for their ability to induce anticapsular and bactericidal antibodies to group c n. meningitidis in adult volunteers. all three vaccines elicited group c-specific serum antibodies. the vaccine derived from the o-a ... | 1979 | 108338 |
| [anti-neisseria meningitidis antibodies, groups a and c, in individuals from urban and rural regions of argentina]. | | 1978 | 108502 |
| electron microscopical study of neisseria meningitidis releasing various amounts of free endotoxin. | a study has been made of the ultrastructure of four strains of neisseria meningitidis which liberate varying amounts of free endotoxin in a chemically-defined, protein-free medium. the two strains which did not release detectable or only sparse amounts of free, filtrable endotoxin were rather uniform in cell size. their cells appeared to be intact and showed a low tendency to aggregate, in addition cells of these strains showed only sporadic loose, trilaminar membranes and blebs, and free membra ... | 1979 | 108919 |
| neisseria meningitidis and neisseria gonorrhoeae bacteremia associated with c6, c7, or c8 deficiency. | we summarize data from 24 previously described or newly diagnosed cases of homozygous deficiency of the sixth, seventh, or eighth components of complement. thirteen of 24 patients had at least one episode, and usually two or more episodes of neisseria meningitidis or neisseria gonorrhoeae bacteremia, or both. deficiency of c6, c7, or c8 is a meaningful risk factor for repeated neisserial bacteremia; conversely, hemolytic complement studies are indicated in patients who develop recurrent neisseri ... | 1979 | 109025 |
| meningococcal conjunctivitis. | meningococcal conjunctivitis is typically described as an acute purulent infection. an atypical case of mild catarrhal conjunctivitis occurred in a 19-year-old college student. the meningococci were identified as neisseria meningitidis, group a, and were isolated from the throats of the patient and her roommate. the conjunctivitis responded rapidly to treatment with sodium sulfacetamide, and it was not treated systemically. a short review of the literature of meningococcal conjunctivitis is pres ... | 1979 | 109067 |
| meningitis due to neisseria meningitidis, group y. | | 1979 | 110137 |
| pili of neisseria meningitidis: effect of media on maintenance of piliation, characteristics of pili, and colonial morphology. | in contrast to information in the literature which indicates that meningococci rapidly lose pili upon cultivation in vitro, we found that piliation of meningococci could be maintained in vitro for 15 or more passages. pili were present on all eight isolates tested, whether from asymptomatic carriers or from subjects with meningococcal disease. complete loss of piliation occurred in the same two strains on two of the three media tested. on one medium (thayer-martin medium with supplement b), ther ... | 1979 | 110692 |
| neisseria meningitidis infection in mice: influence of iron, variations in virulence among strains, and pathology. | the influence of iron on neisseria meningitidis infection in c-57 mice was examined. iron sulfate, ferric ammonium citrate, and iron sorbitol citrate all proved to be too toxic for use as infection-enhancing agents. iron dextran displayed an extremely low toxicity, enhanced infection in a dose-dependent manner, and resulted in infection enhancement factors approaching 10(9) for virulent strains of n. meningitidis. fatal iron dextran-enhanced infection was shown to be reversible by in vivo chelat ... | 1979 | 110703 |
| [demonstration of neisseria meningitides in the urogenital tract]. | the occurence of neisseria meningitidis in the urogenital tract is a rarity but nevertheless this may lead to a false diagnosis of gonorrhea, even when the thayer-martin-selective-medium is used and oxidase-reaction is performed. this is demonstrated in our case. a complete diagnosis, including sugar-fermentation, is necessary, when gram-negative diplococci are isolated from urogenital swabs. in the case neisseria meningitidis is isolated, it is not required by law to report it to the public hea ... | 1979 | 110728 |
| hen fluorescein-labeled gonococcal lipopolysaccharide antibody in the delayed fluorescent antibody technique for the confirmation of neisseria gonorrhoeae. | a fluorescent antibody reagent (termed anti-lps conjugate) was prepared from sera obtained from hens immunized with gonococcal r-type lipopolysaccharide. the reagent was absorbed with formalin-treated cells of neisseria meningitidis. the anti-lps conjugate gave uniform brilliant staining of neisseria gonorrhoeae with little background fluorescence, thus making interpretation and reading of fluorescence simple. the conjugate did not significantly stain cultures of n. meningitidis, neisseria lacta ... | 1979 | 110824 |
| meningitis and bacteremia due to neisseria meningitidis: clinical and laboratory findings in 69 cases from orebro county, 1965 to 1977. | the records for 69 patients with meningococcal disease during 13 years in orebro county were reviewed. the mean incidence during this period was 2.0/100 000 persons/year. the age group less than 1 year had the highest incidence, 15/100 000 infants/year. the mortality was 7.2% and sequelae were found in 13% of the patients. four factors were significantly more frequent in the 7 patients with psycho-neurological sequelae: (1) age greater than 40 years; (2) a condition prior to meningococcal diseas ... | 1979 | 111345 |
| [sensitivity to antibiotics of the "neisseria meningitidis" strains isolated among healthy subjects (author's transl)]. | in 1977, from march through april, 16 strains of n. meningitidis were isolated among 400 healthy subjects: 334 school-children 6-11 years of age and 66 adults, with a carrier rate of 3.85% and 4.5% respectively. the serological identification carried out by slide agglutination showed: 1 strain belonging to serogroup a; 4 strains to serogroup b; 1 strain to serogroup c; 1 strain to serogroup y; 3 strains were autoagglutinable and 5 nonagglutinable. minimum inhibiting concentrations of sulfamethox ... | 1978 | 111628 |
| individual susceptibility to neisserial infection? | specimens from genital, anorectal, and pharyngeal sites from 1671 men and 1419 women were cultured for neisseria gonorrhoeae. pharyngeal specimens were also cultured for neisseria meningitidis, n. gonnorrhoeae was isolated from a genital site 2.7 times more often in men and 1.8 times more in women who also carried meningococci in their pharynx than from those who did not; the meningococcus was isolated 3.4 times more often from men and 2.0 times more often from women with genital gonorrhoea than ... | 1979 | 111765 |
| neisseria meningitidis bacteremia in association with deficiency of the sixth component of complement. | the serum of a 26-year-old black man with a recent episode of meningococcemia complicated by meningitis and arthritis was found to lack hemolytic complement activity. the sixth component of complement was not detected by functional or immunochemical assays whereas other components were normal by hemolytic assay. his fresh acute-phase serum lacked complement-mediated bactericidal activity against the homologous strain of neisseria meningitidis, but the addition of fresh normal serum or purified c ... | 1979 | 112055 |
| disease due to serogroup w135 neisseria meningitidis. | five cases of disseminated meningococcal disease due to serogroup w135 neisseria meningitidis are presented. the cases ranged in age from 16 months to 23 years, and spanned a clinical spectrum from mild meningitis without rash or evidence of meningococcal septicemia to severe meningoencephalitis with fulminant meningococcemia, disseminated intravascular coagulation, and death. these cases demonstrate that serogroup w135 n meningitidis is fully pathogenic for man and capable of producing the full ... | 1979 | 112572 |
| meningococcal group y pneumonia in an adolescent female. | neisseria meningitidis group y has been considered to be an uncommon pathogen. meningococcal group y disease has recently been reported with increased frequency in military training camps coincident with the routine use of meningococcal group c vaccine. pneumonia produced by the group y organism may mimic disease caused by common respiratory tract pathogens, and isolation by routine methods may be difficult. a 16-year-old asthmatic female developed lobar pneumonia secondary to n meningitidis gro ... | 1979 | 112573 |
| evaluation of the antiserum agar method for the serogroup identification of neisseria meningitidis. | the antiserum agar method (asa), which is based on the formation of immunoprecipitates around bacterial growth on agar containing meningococcal hyperimmune horse serum, was evaluated for serogroup identification of neisseria meningitidis. four hundred meningococcal stains were serogrouped by asa employing horse antisera to serogroups a, b, c, y, w135, z, and 29e and compared to serogroup identification by bacterial slide agglutination (ba) employing rabbit antisera. overall, there was 95% agreem ... | 1979 | 113070 |
| neisseria meningitidis. probable pathogen in two related cases of urethritis, epididymitis, and acute pelvic inflammatory disease. | | 1979 | 113569 |
| neisseria meningitidis urethritis. a case report. | | 1979 | 113570 |
| rapid serogroup identification of neisseria meningitidis by using antiserum agar: prevalence of serotypes in a disease-free military population. | nasopharyngeal cultures from 414 marines were plated directly onto antiserum agar containing the antibiotics vancomycin, colistin, and nystatin for meningococcal isolation and serogroup identification. meningococci were isolated from 267 marines, giving a carrier prevalence of 64.5%. a total of 58% of the isolates could be placed into serogroups; of these 22.3% were group b, 4.7% were group c, 25.7% were group y, 24.3% were group w135, and 23.0% were group 29e. no serogroup a organisms were reco ... | 1979 | 114532 |
| outer membrane antigens of neisseria meningitidis group b serotype 2 studied by crossed immunoelectrophoresis. | this study shows that the capsular polysaccharide, protein, and lipopolysaccharide antigens from the outer membrane of neisseria meningitidis group b serotype 2 may be identified by crossed immunoelectrophoresis. by using this technique, seven precipitates were resolved when outer membrane preparations were reacted against goat anti-whole cell group b type 2 antiserum. most of these precipitates were identified by comparison with purified reference preparations. different outer membrane preparat ... | 1979 | 115791 |
| inhibition of the growth of neisseria meningitidis by reduced ferritin and other iron-binding agents. | serogroups of n. meningitidis were characterized as virulent or avirulent according to their capacity to establish meningococcal infection in mice. an agar plate diffusion technique demonstrated that iron had a definite growth-supporting role for both of these meningococcal types. the avirulent strains could use ionic or chelated iron as well as the virulent strains. iron-reversible growth inhibition occurred to the same extent for both bacterial types in the presence of the synthetic iron-chela ... | 1979 | 115792 |
| urethritis attributable to neisseria meningitidis, group x: a case report. | neisseria meningitidis, group x, was recovered from the anterior urethra of a 27-year-old male patient with urethritis. this isolate represents the first reported recovery of this organism from this anatomic site in association with this disease. he was treated with probenicid by mouth, followed by 4.8 million units of aqueous procaine penicillin g injected intramuscularly and became asymptomatic in three days. his recovery strongly indicates that the meningococcus was the etiologic agent of the ... | 1979 | 116007 |
| frequency of simultaneous carriage of neisseria gonorrhoeae and neisseria meningitidis. | gonococci were found by culture in 112 of 442 patients suspected of having gonorrhoea. meningococci were cultured from the pharynx more than twice as often in patients harbouring gonococci (26%) than in patients from whom gonococci could not be cultured (11%). these results could indicate an individual susceptibility to neisseria organisms or they could be caused by a difference in the behaviour of patients with and without gonorrhoea, the conduct of the former making them not only more likely t ... | 1979 | 116705 |
| heterogenity of serotypes of neisseria meningitidis that cause endemic disease. | three collections of strains of neisseria meningitidis that caused meningococcal disease during nonepidemic periods were serotyped to determine whether serotypes that cause endemic disease are more heterogeneous than those responsible for epidemic disease. thirty-four strains isolated from pediatric patients in houston, texas, from february 1977 to march 1978 were of three separate serogroups and 11 serotypes; 27 contemporary (1977-1978) strains from predominantly military populations, obtained ... | 1979 | 117060 |
| evaluation of bactericidal assay in serotyping neisseria meningitidis group a. | seventeen of 22 group a meningococcal strains were separated into four serotypes by a microbactericidal assay using group a antisera. in addition, 6 of 19 of these strains were killed by a group b antiserum and similar cidal patterns were obtained with a group c antiserum. there was little correlation between type and case or carrier isolates, or between types and geographical location. | 1979 | 118800 |
| serological grouping of meningococci and encapsulated haemophilus influenzae strains by latex agglutination. | the latex agglutination method, utilizing antibody-coated latex particles, was adapted for serogrouping of neisseria meningitidis and serotyping of encapsulated haemophilus influenzae strains from agar plates. it was found to give more clear-cut results than conventional slide agglutination. a 100% agreement with the antiserum agar method was found for all strains isolated from blood or cerebrospinal fluid. many meningococcal strains from nasopharyngeal carriers are autoagglutinable, but some of ... | 1979 | 118981 |
| kinetics of antibody production to group a and group c meningococcal polysaccharide vaccines administered during the first six years of life: prospects for routine immunization of infants and children. | a cohort of infants, previously immunized once or twice between three and 12 months of age with vaccines containing polysaccharide of groups a and c neisseria meningitidis, received boosters at two and five and one-half years of age with bivalent a/c vaccine. antibody concentrations were measured by a radioactive antigen-binding assay. concentrations of antibody to groups a and c n. meningitidis were 5.59 and 2.86 microgram/ml, respectively, by four years of age. after booster immunization at fi ... | 1979 | 118997 |
| the role of natural igg and complement in the phagocytosis of type 4 neisseria gonorrhoeae by human polymorphonuclear leukocytes. | the role of human serum components in the phagocytosis of logarithmic-phase type 4 neisseria gonorrhoeae by human polymorphononuclear leukocytes was investigated. the requirement of fresh normal human serum (fhs) for optimal phagocytosis and the fixation of human immunoglobulin (igg) and complenet (c3) to the gonococcal cell surface suggested that both serum factors participate in the phagocytosis of these organisms. the percentage of neutrophils containing ingested organisms was directly propor ... | 1979 | 118998 |
| [meningococcus profilaxis (author's transl)]. | in a general hospital in san sebastian, 96 cases of neisseria meningitidis infections were detected in a two years period. by the use of the disk diffusion method, we found that all causative meningococcal strains but 4 were resistant to sulfonamide (with a 300 microgram sulfadiazine disk, all isolates with a zone diameter of less than 20 mm were considered to be resistant of sulfadiazine, whereas those with zone diameters of greater than 30 mm were considered susceptible). no rifampin nor minoc ... | 1979 | 119117 |
| culture diagnosis of meningococcal carriers: yield from different sites and influence of storage in transport medium. | different specimens and techniques have been used in the diagnosis of carriers of neisseria meningitidis, reflecting the uncertainty about the optimal diagnostic procedure. in the present investigation the culture yield of meningococci from throat specimens was compared to that from nasopharyngeal speimens in 178 persons: 44 carriers were diagnosed. all of them were detected by culture of throat specimens while 34% of them would have remained undiagnosed if only nasopharyngeal specimens had been ... | 1979 | 119792 |
| [different serogroups of neisseria meningitidis in sick people and in the normal population]. | | 1979 | 122281 |
| [differential traits and antigenic properties of the s- and r-forms of neisseria meningitidis]. | the authors demonstrated a marked dissociation of the neisseria meningitis cultures and the capacity to their rapid change from s- to r-forms. differential signs of the s- and r-forms are described. differences in the degree of dissociation of the cultures depending on their origin were shown: strains isolated from the cerebrospinal fluid and the blood of patients with meningitis proved to be less dissociated, and those isolated from carriers--more dissociated. transfers from media to media and ... | 1975 | 128964 |
| [detection of neisseria meningitidis in pharyngeal exudates using a new culture media: agar n.m.8]. | | 1977 | 141294 |
| susceptibility-resistance pattern of neisseria meningitidis isolated in the province of ontario, canada. | a total of 522 n. meningitidis cultures isolated from different sources (cerebrospinal fluid, sputum, blood, naso-pharyngeal, uro-genital area, eye and rectal samples) between the years 1976-1977 in ontario, were tested for their susceptibility pattern to 8 antibacterial drugs. among the 522 cultures, 24.8% showed resistance to sulfadiazine (10-100 micrograns/ml) and all n. meningitidis cultures (100%) were resistant to spectinomycin (15 micrograms/ml); this feature could be used as one of the c ... | 1979 | 159654 |
| structural determination of the sialic acid polysaccharide antigens of neisseria meningitidis serogroups b and c with carbon 13 nuclear magnetic resonance. | the application of 13-c nuclear magnetic resonance to the analysis of some sialic acid-containing meningococcal polysaccharide antigens is described. complete assignments of the spectra of both the native serogroup b and the de-o-acetylated serogroup c polysaccharides have been made. these assignments were based on the corresponding data for some related monomers (sialic acid and its alpha-and beta-methylglycosides) and on supportive chemical evidence. the data indicate that the serogroup b poly ... | 1975 | 163259 |
| primary meningococcal pneumonia. | three cases of pneumonia caused by neisseria meningitidis group y are reported. from the results of these cases, the following conclusions were made. n. meningitidis probably can cause serious infection without preceding blood stream invasion. primary meningococcal pneumonia is not rare; it has no distinctive clinical presentation; and it may not be recognized by routine expectorated sputum cultures. in addition, it may be associated with recent influenzal and adenoviral infections. lastly, meni ... | 1975 | 164144 |
| microcalorimetry as a tool for evaluation of blood culture media. | evaluation of optimal compositions of blood culture media has called for extensive and laborious work in comparative studies of large series of clincal specimens. bacterial growth is accompanied by heat production, and calorimetry provides an analytical tool for its detection and quantification. a twin microcalorimeter of the heat conduction type was used to register heat effects in experimentally infected blood cultures. when studying escherichia coli and staphylococcus aureus, larger heat effe ... | 1977 | 192760 |
| gelatin neutralization of the inhibitory effect of sodium polyanethol sulfonate on neisseria meningitidis in blood culture media. | the inhibitory effect of sodium polyanethol sulfonate (0.05%) upon growth of neisseria meningitidis was found to be neutralized by adding gelatin (l.1%) to the growth medium. the neutralizing effect was demonstrated in solid medium, as well as in nutrient broth for blood cultures. the findings parallel those of wilkins and west (6) regarding gelatin neutralization of the inhibitory effect of sodium polyanethol sulfonate on peptostreptococcus anaerobius. | 1977 | 195978 |
| rapid identification of neisseria gonorrhoeae and neisseria meningitidis by using enzymatic profiles. | the enzymatic profiles of neisseria gonorrhoeae, n. meningitidis, and related species were determined, using a total of 48 chromogenic substrates. enzyme classes assayed for included glycosidases, aminopeptidases, phosphoamidases, proteases, lipases, esterases, and aryl sulfatase. a final test selection of 10 substrates, based upon their differential and reproducible characteristics, allowed the separation of n. gonorrhoeae and n. meningitidis from each other and from all species tested within 4 ... | 1978 | 203607 |