properties of the nadp+-dependent isocitrate dehydrogenases of acinetobacter calcoaceticus. 1975849
pyridine nucleotide-independent oxidation of long chain aliphatic alcohols by an enzyme of acinetobacter calcoaceticus. 1975850
preparations and properties of ribonucleic acid polymerase from acinetobacter calcoaceticus.deoxyribonucleic acid (dna)-dependent ribonucleic acid (rna) polymerase (ec from acinetobacter calcoaceticus was purified to apparent homogeneity and its properties were compared with those of the escherichia coli b enzyme. the molecular weights of the two native active enzymes as well as their alpha and beta subunits appeared to be similar. no subunit corresponding to that of sigma from e. coli was found, and furthermore no separation between the beta subunits could be detected by gel ...19761380
inhibition of the malic enzyme from acinetobacter calcoaceticus by nadph and nadh.the malic enzyme enriched from acinetobacter calcoaceticus is inhibited by nadph and nadh. the inhibition afforded by the reduced coenzymes is not affected by nad+, amp and 3'.5'-amp. against l-malate, nadph inhibits the enzyme in a noncompetitive linear fashion (ki = 1.5 x 10(-4) m), against nadp+, competitively linearly (ki = 5.0 x 10(-5) m). while nadph acted as a product inhibitor, nadh seems to be an allosteric effector of the malic enzyme, because with l-malate as the variable substrate in ...19759762
purification and various properties of nadp+-dependent alcohol dehydrogenase from acinetobacter calcoaceticus.the constitutive nadp+-dependent alcohol dehydrogenase from acinetobacter calcoaceticus can be accumulated about 50 fold in 3 purification steps. the end-product shows in the analytical polyacrylamide gel electrophoresis only one active enzyme band. the molecular weight of the enzyme was determined to be 235,000 by gel chromatography on sephadex g 200, the smallest subunit shows a molecular weight of 61 000 on sds electrophoresis. the isoelectric point is at 5.84. the km values determined with p ...197614464
[oxidation of homologous aliphatic aldehydes by membrane-bound and solubilized aldehyde dehydrogenase from acinetobacter calcoaceticus]. 197832669
[properties of rubredoxin reductase from the alkane-assimilating bacterium acinetobacter calcoaceticus]. 197944771
fine structure and distribution of extracellular polymer surrounding selected aerobic bacteria.the structure and distribution of extracellular polymer surrounding bacillus circulans, diplococcus (streptococcus) pneumoniae, streptococcus salivarius, staphylococcus aureus, klebsiella pneumoniae, pseudomonas aeruginosa, herella vaginacola (acinetobacter calcoaceticus), and agrobacterium tumefaciens were studied by electron microscopy. a modified ruthenium red staining procedure was used to examine the fine structure of capsule and slime. freeze-etching and critical-point drying were used to ...197546774
catechol 1,2-dioxygenase from acinetobacter calcoaceticus: purification and properties.procedures for the purification of catechol 1,2-dioxygenase from extracts of acinetobacter calcoaceticus strain adp-96 are described. the purified enzyme was homogeneous as judged by ultracentrifugation and acrylamide gel electrophoresis. the enzyme contained 2 g-atoms of iron per mol of protein. the enzyme had a broad substrate specificity and catalyzed the oxidation of catechol, 4-methylcatechol, 3-methylcatechol, and 3-isopropyl catechol. the activity of the enzyme was inhibited by heavy meta ...197658860
community-acquired acinetobacter calcoaceticus var anitratus pneumonia.two patients had community-acquired acinetobacter calcoaceticus var anitratus pneumonia. both patients were alcoholic and one was cirrhotic. one patient died and the other received two weeks of gentamicin therapy and survived. misinterpretation of the sputum gram stain delayed diagnosis and institution of proper therapy in both cases. in addition to organisms sensitive to penicillins such as neisseria or haemophilus, acinetobacter must be considered in the differential diagnosis of community-acq ...197770540
studies on the oxidation of octane by acinetobacter calcoaceticus and flavobacterium devorans strains. 197994750
microbial and chemical transformations of some 12,13-epoxytrichothec-9,10-enes.resting cells of streptomyces griseus, mucor mucedo, and a growing culture of acinetobacter calcoaceticus when mixed with compounds related to 12,13-epoxytrichothec-9-ene-4beta,15-diacetoxy-3alpha-ol(anguidine) produced a series of derivatives that were either partially hydrolyzed or selectively acylated. these derivatives showed marked differences in activities as assayed by antifungal and tissue culture cytotoxicity tests.1978100053
[infection by glucose-nonfermenting, gram-negative rods--infection by acinetobacter calcoaceticus]. 1977101606
the behaviour of nad+ and nadh in acinetobacter calcoaceticus during n-alkane assimilation.the behaviour of the nicotinamide adenine dinucleotides nad+ and nadh in acinetobacter calcoaceticus during n-alkane assimilation was studied, acetate and succinate being used as reference carbon sources. the intracellular concentration of the two nucleotides was found to increase during the exponential growth phase, reaching its maximum in the phase of decreasing growth rates. in the exponential phase, the nad+/nadh quotients were less than 1 and showed only unimportant variations. in the phase ...1975170172
in vitro and in vivo activities of minocycline and other antibiotics against acinetobacter (herellea-mima).minocycline was the most active of six antibiotics tested against 65 clinical isolates of acinetobacter calcoaceticus (syn.: herellea, mima) received from six medical centers. in the bauer-kirby disk susceptibility test, all isolates were rated susceptible to minocycline, gentamicin, and polymyxin; 25% were resistant to tetracycline. in agar dilution tests, minocycline was two to four times more potent than gentamicin or polymyxin and eight times more potent than tetracycline. ampicillin and cep ...1976176936
solubilization studies and properties of particulate nad-independent malate dehydrogenase of acinetobacter calcoaceticus (n.c.i.b. 8250). 1977196960
regulation of synthesis of benzyl alcohol dehydrogenase in acinetobacter calcoaceticus ncib8250.specific activity of benzyl alcohol dehydrogenase in carbon-limited continuous cultures was at a maximum at a specific growth rate of 0.2 h-1, but fell off at lower and higher growth rates. the specific activity in nitrogen-limited cultures was always lower and was inversely proportional to growth rate. there was severe repression of benzyl alcohol dehydrogenase during metabolism of l(+)-mandelate or phenylglyoxylate in batch cultures. synthesis of benzyl alcohol dehydrogenase was followed in ex ...1977201725
phosphatase activity of aerobic and facultative anaerobic bacteria.1115 strains of aerobic and facultatively anaerobic bacteria were tested for phosphatase activity by a conventional plate method and a microtest. the microtest was devised to allow results to be read after 4 h cultivation. phosphatase activity was found in wide range of species and strains. besides staphylococci, where the test for phosphatase is successfully used, it may be applied as one of the valuable tests for the differentiation of the following species: bacillus cereus, b. licheniformis, ...1978216188
[kinetic and regulatory properties of malate dehydrogenase from acinetobacter calcoaceticus]. 1978216197
nadh and nadph-viologen reductases from acinetobacter calcoaceticus.three pyridine nucleotide-dependent diaphorases have been isolated from acinetobacter calcoaceticus cells and partially characterized. two of them, with molecular weights of 165,000 and 57,000, utilize nadph as electron donor whereas the third one (mw = 57,000) is specific for nadh. oxidized viologen dyes, flavin nucleotides, dichlorophenol indophenol and ferricyanide can act with efficiency as acceptors in the reaction mediated by these diaphorases. the diaphorase activities have been character ...1979223203
the influence of changes in the environment on twitching examining medium composition and cultural conditions quantitatively it was possible to define conditions suitable to bring twitching motility about. such conditions include the use of freshly poured, relatively thick and only slightly dried plates of a dilute medium in which the agar concentration is not too high. incubation should take place in a humid atmosphere. in fact, everything points to the humidity as a factor of the utmost importance. using strains of acinetobacter calcoaceticus, it ...1975239520
outbreak of a multiply resistant acinetobacter in a surgical intensive care unit: epidemiology and control.during january, 1976 seven patients in an sicu became colonized or infected with acinetobacter calcoaceticus (variation, anitratum) at an attack rate of 12.5 per cent. the organism showed a marked reduction in antimicrobial sensitivity from previous experience. comparison with 34 uninfected control patients indicated that intubation and continuous ventilatory assistance were significantly associated with acquiring the organism (p = 0.0154). acinetobacter was cultured from nurses' hands, ambu ada ...1978248372
ecological effects of a deodorant and a plain soap upon human skin bacteria.the effects of a commercial trichlorocarbanilide-containing deodorant soap and a commercial plain soap upon the cutaneous flora of individuals were compared. using a cross-over design, 21 volunteers (10 women and 11 men) washed their forearms at least once a day with one soap for 3 weeks and then switched soaps for another 4 weeks use. by analysis of variance no significant difference in total colony counts was noted among individuals in their use of the two soaps. with the exception of individu ...1977264493
origins of metabolic diversity: evolutionary divergence by sequence repetition.recurring patterns of primary structure have been observed in enzymes that mediate sequential metabolic reactions in bacteria. the enzymes, muconolactone delta-isomerase [(+)-4-hydroxy-4-carboxymethylisocrotonolactone delta(2)-delta(3)-isomerase, ec] and beta-ketoadipate enol-lactone hydrolase [4-carboxymethylbut-3-enolide(1,4)enol-lactone-hydrolase, ec], have been coselected in bacterial populations because the isomerase can confer no nutritional advantage in the absence of the ...1979291059
prosthetic valve endocarditis by opportunistic pathogens.the incidence of endocarditis produced by the so-called "opportunists" as a complication of prosthetic valve surgery is progressively increasing in frequency and gradually transforming the clinical picture habitually associated with this disease. we report six cases of endocarditis produced by opportunistic microorganisms (two cases by candida, and the remaining by serratia, actinobacillus, acinetobacter calcoaceticus, and bacteroides fragilis, and by corynebacterium diphtheriae) in four male an ...1977319774
host factor for coliphage q beta rna replication: presence in procaryotes and association with the 30s ribosomal subunit in escherichia coli.the host factor required for in vitro coliphage q beta rna replication, a heat-stable rna binding protein present in uninfected escherichia coli, has been detected by both immunological and functional tests in acinetobacter calcoaceticus, klebsiella pneumoniae, pseudomonas aeruginosa and pseudomonas putida. it was not detectable by these criteria in bacillus stearothermophilus, bacillus subtilis, caulobacter crescentus, micrococcus lysodeikticus, rhodopseudomonas capsulata or saccharomyces cerev ...1977329101
[acinetobacter calcoaceticus as the possible cause of bronchopneumonia in rabbits]. 1978352659
evaluation of the oxi/ferm tube system for identification of nonfermentative gram-negative bacilli.the oxi/ferm tube system designed for identification of oxidative-fermentative gram-negative bacteria was evaluated for identification of nonfermentative gram-negative bacilli by comparing it with the conventional system. the nine biochemical reactions used as the initial tests in the oxi/ferm tube system showed a close agreement with the same conventional reactions. however, the system was only 41% accurate to species or biotype for complete identification of 239 isolates which were well distri ...1978353066
evaluation of the api 20e system for identification of nonfermentative gram-negative bacteria.the api 20e system for enterobacteriaceae, recently broadened to include identification of nonfermentative gram-negative bacteria, was evaluated and compared with the conventional method for complete identification of 221 nonfermenters, which were well distributed into 48 species or biotypes and included organisms not listed in the api 20e data base. the results of 16 tests common to both systems were in close agreement. the api 20e system correctly identified 71 (43%) of the 165 organisms inclu ...1978353067
nosocomial respiratory tract infection and colonization with acinetobacter calcoaceticus. epidemiologic characteristics.nosocomial respiratory tract infection with acinetobacter calcoaceticus occurs frequently in many hospitals. an outbreak of respiratory tract infections in an intensive care unit provided an opportunity to study clinical and epidemiologic characteristics of such infections. retrospective studies demonstrated that a. calcoaceticus in sputum was significantly associated with endotracheal intubation (p = 0.03) and continuous positive pressure ventilation (p less than 0.02). after control measures h ...1978362918
identification of gram-negative non-fermenters and oxidase-positive fermenters by the oxi/ferm tube.since the recent introduction of the roche oxi/ferm tube to the uk two identification schemes have been developed by the manufacturer for use with the kit. we evaluated the success of these two schemes in identifying 222 predominantly culture collection strains belonging to 45 taxa of non-fermenters and nine taxa of oxidase-positive fermenters. the strains were chosen to represent all the taxa included in the two identification schemes developed by the manufacturer and we have therefore been abl ...1979372251
acinetobacter calcoaceticus variety anitratus: an increasing nosocomial problem.acinetobacter calcoaceticus var anitratus is an increasing nosocomial problem in some institutions. duirng a recent 16-month period, we observed 15 patients with blood isolates of this organism. thirteen of these patients acquired the organism from the hospital environment. eleven of these represented real disease and concurrent growth of the organism from another site was predictive of infection (p less than 0.03). a review of laboratory records showed that there was a three-fold increase in no ...1979426000
aminoglycoside-modifying enzymes among clinical isolates of acinetobacter calcoaceticus subsp. anitratus (herellea vaginicola): explanation for high-level aminoglycoside resistance.acinetobacter calcoaceticus subsp. anitratus (herellea vaginicola) is an important cause of nosocomial infection in our hospital where a. calcoaceticus subsp. anitratus is the most frequently isolated gram-negative species resistant to one or more of the aminoglycoside antibiotics. of 167 strains tested for susceptibility to aminoglycosides, only 6 strains were found that were resistant to >/=128 mug of kanamycin per ml; all others were susceptible to </=32 mug/ml. five of these six strains were ...1979426512
acinetobacter calcoaceticus: a nosocomial pathogen with an unusual seasonal pattern. 1979448188
ventriculitis from acinetobacter calcoaceticus variant anitratus. 1979448387
community-acquired acinetobacter pneumonia.acinetobacter calcoaceticus var anitratus, a nonfermentative grampnegative bacillus, has been infrequently reported as a cause of community-acquired pneumonia. in this paper we describe the course of six recent patients with community-acquired, bacteremic pneumonia due to this organism and review the six previously reported cases. our experience suggests this organism is a more common cause of community-acquired pneumonia than previously thought. acinetobacter pneumonia occurs in older persons w ...1979463916
a hand infection from a. calcoaceticus (m. polymorpha).acinetobacter calcoaceticus (mima polymorpha) is not a well known organism, and its identification can be confusing due to its variable morphology and sensitivity pattern. in a joint infection, delay in identification or appropriate treatment can result in added morbidity. an a. calcoaceticus (mima) infection in the hand seems not to have been previously reported. the present case involving a finger joint infection illustrates the pathogenicity of a. calcoaceticus (mima) and problems of treatmen ...1979477074
glucose dehydrogenase from acinetobacter calcoaceticus: a 'quinoprotein'. 1979520586
serological cross-reactions between acinetobacter calcoaceticus and chlamydiae.a cross-reaction between acinetobacter calcoaceticus and chlamydiae is described. a water-soluble, heat stable, non-dialyzable antigen was extracted from acinetobacter species by boiling. this antigen fixed complement in the presence of homologous hyperimmune sera from rabbits or guinea pigs and in the presence of heterologous human or hyperimmunized animal sera containing chlamydial antibodies. hyperimmune antisera to the extracted antigen, or to suspensions of live acinetobacters, also reacted ...1979521480
in vitro activity of three tetracycline antibiotics against acinetobacter calcoaceticus subsp. anitratus.the in vitro activity of three tetracycline antibiotics against 127 strains of acinetobacter calcoaceticus (herella vaginicola) were compared. almost all strains were susceptible to minocycline and doxycycline, whereas most strains were resistant to tetracycline.1979526013
[structural features of acinetobacter calcoaceticus k-9 cells assimilating ethanol at different rates of medium dilution]. 1979530115
[uptake of organic acids by acinetobacter calcoaceticus]. 1979543155
[a case of meningitis due to acinetobacter calcoaceticus].meningitis due to acinetobacter calcoaceticus is rare. these rare bacteria were isolated from culture fluid in patient resembling to neisseria meningitis with clinical and laboratory findings. the patient was treated with sensitive antibiotic.1979555785
[isolation and identification of acinetobacter calcoaceticus from clinical materials and it's clinial value in respiratory infectious diseases (author's transl)]. 1977559145
chronic haematuria caused by acinetobacter calcoaceticus. 1978567878
room humidifiers as the source of acinetobacter infections.twenty-four patients contracted systemic infections with acinetobacter calcoaceticus during a four-month period. unheated room humidifiers at the patients' bedsides were implicated as the source of infection in six. the outbreak was terminated with the removal of the humidifers.1977576319
critique: "some aspects of an epidemiological study of nosocomial infections due to acinetobacter calcoaceticus (herellea) featuring bacteriocin typing". 1977585596
some aspects of an epidemiological study of nosocomial infections due to acinetobacter calcoaceticus (herellea) featuring bacteriocin typing. 1977585613
[characteristics of growth and changes in the ultrafine structure of bacteria in the course of continuous cultivation on media containing ethanol].a large number of bacterial strains assimilating chemical ethanol has been isolated using an original technique. active growth of strains belonging to the genera pseudomonas and, particularly, acinetobacter was registered on mineral media containing ethanol. a mathematical model was constructed select a strain of acinetobacter calcoaceticus k-9 during its continuous cultivation on media containing ethanol. the model makes it possible to determine conditions for producing a present amount of the ...1977600096
[uptake of acetate by acinetobacter calcoaceticus].the uptake of acetate by intact nongrowing cells of acinetobacter calcoaceticus was studied in dependence on the c-source (acetate, n-alcanes, yeast extract, succinate, l-malate) and the growth phase. single kinetic parameters of acetate uptake were determined. the best acetate uptake was observed with cells cultivated with acetate as the only c-source. bacteria in the early growth phase were found to transfer acetate twice as fast as cells of the late logarithmic growth phase. the uptake of ace ...1977614747
genetic analysis of acinetobacter calcoaceticus proline auxotrophs.twenty-six acinetobacter calcoaceticus proline auxotrophs were isolated after ethyl methane sulfonate mutagenesis. studies using the efficient transformation system of this organism indicate that the mutations comprise therr genetically distinct groups.1978618847
equine myositis and septicemia caused by acinetobacter calcoaceticus infection.myositis and septicemia caused by acinetobacter calcoaceticus were diagnosed in a mare. the infection was characterized clinically by ventral swelling and edema, diarrhea, listlessness, and rectal temperature of 39.4 c. the mare was treated symptomatically for 2 days but died on the 3rd day. conditions seen at necropsy were myositis, enteritis, typhlitis, colitis, and hepatitis. lymph nodes were moderately enlarged throughout the body. gross lesions in musculature were edema, scarring, petechiae ...1978621183
utilization of oxalacetate by acinetobacter calcoaceticus: evidence for coupling between malic enzyme and malic dehydrogenase.growth of acinetobacter calcoaceticus strain bd413 in malate-mineral medium resulted in the excretion of large quantities of oxalacetate. malate was virtually depleted by the time the cell density reached 60% of its final value; most of the remaining growth took place at the expense of oxalacetate. experiments in which oxalacetate was used as the initial substrate showed that pyruvate was not utilized until most of the oxalacetate disappeared. the generation time for growth on malate or oxalacet ...1978627536
chromosome mapping in acinetobacter calcoaceticus.rp4-mediated conjugation was used to map the loci of 23 different mutations on a circular linkage group in acinetobacter calcoaceticus ebf65/65. the resulting genetic map indicated that the chromosomal organization of a. calcoaceticus differed from that of members of the enteric group of bacteria and was similar to pseudomonas in showing an absence of clustering of functionally related genes.1978632801
acinetobacter calcoaceticus outbreak associated with peritoneal dialysis.investigation of an outbreak of contamination of dialysis drainage fluid with acinetobacter calcoaceticus var. anitratus identified a previously unrecognized source for dialysis associated infections. over a 4-month period, 25 peritoneal dialysis treatments were administered to 13 hospital patients. of the 25 treatments for which culture results were available, 14 were associated with dialysis drainage fluid cultures positive for a. calcoaceticus. a water bath used to warm bottles of peritoneal ...1978645702
mutants of acinetobacter calcoaceticus ncib 8250 constitutive for the mandelate enzymes.methods for isolating mutants of acinetobacter calcoaceticus ncib 8250 constitutive for the mandelate enzymes were compared. non-inducing substrates were not available. continuous culture gave no stable constitutive mutants. alternate culture in inducing (l-mandelate) and non-inducing (l-glutamate) media led to the isolation of mutants mesoconstitutive for l-mandelate dehydrogenase and phenylgloxylate carboxy-lyase, and hyperinducible for benzaldehyde dehydrogenase i. screening of possible anti- ...1978670930
chronic haematuria caused by acinetobacter calcoaceticus in a race horse. 1978676010
search for acinetobacter calcoaceticus subsp. anitratus: enrichment of fecal samples.none of 50 healthy individuals and only 2 of 50 hospitalized patients (with acinetobacter anitratus elsewhere) yielded a. anitratus on enrichment from fecal samples.1978701469
[cytochrome composition of acinetobacter calcoaceticus].the qualitative and quantitative composition of cytochromes in intact cells of acinetobacter calcoaceticus and in particle fractions obtained from cells following ultrasonic treatment by differential centrifugation were studied using spectrophotometric methods. acinetobacter calcoaceticus contains cytochrome b, cytochrome o and low amounts of cytochrome d. both the absolute content of cytochrome b and o and the relative composition do not essentially vary with the carbon source used (hexadecane, ...1978706933
[bacteriemia due to acinetobacter calcoaceticus var. anitratus (author's transl)].a serious bacteremia caused by acinetobacter calcoaceticus var. anitratus could be observed. from the clinical point of view the most important symptoms were shivering, high tempeature up to 41 degrees c and clinical signs of acute hepatitis. there were pathological changes in the eeg. certain signs of an outlayer damage of the heart could be seem in the ecg. the pathogenic microorganism isolated from the blood culture media was classified. specific antibodies--agglutinines and precipitines--wer ...1978723766
rp4-mediated conjugation in acinetobacter calcoaceticus.the p class r factor rp4 was transferred from escherichia coli k12 to acinetobacter calcoaceticus. rp4 conferred similar levels of antibiotic resistance in a. calcoaceticus to those in the e. coli k12 donor. only slight instability of rp4 in a. calcoaceticus was detected. transfer of rp4 between strains of a. calcoaceticus was by conjugation and was accompanied by transfer of chromosomal genes. possible polarity of marker transfer was observed and linkage between a number of chromosomal markers ...1976778327
conversion of escherichia coli rna polymerase to a template independent enzyme.preparations of rna polymerase (e.c. from uninfected escherichia coli, t4 infected escherichia coli, and acinetobacter calcoaceticus when centrifuged in sucrose gradients in the absence of magnesium ions gave rise to five peaks, all of which were able to form polymers from ribonucleoside 5'-triphosphates in the absence of template or primer. all of the peaks obtained from the escherichia coli enzyme appeared to contain the subunit alpha and beta and, in addition, polypeptides which appe ...1976787928
susceptibility of acinetobacter calcoaceticus var. anitratus (herellea vaginicola) to minocycline.the in vitro activity of minocycline against acinetobacter calcoaceticus var. anitratus (herellea vaginicola) was examined. all strains of a. anitratus were inhibited by minocycline at concentrations of 0.4 mug/ml or less. the strains studied were considerably more susceptible to minocycline than tetracycline. no difference between the susceptibility to minocycline and tetracycline was seen with escherichia coli, klebsiella pneumoniae, enterobacter and proteus sp. a regression line of the log mi ...1976791084
mechanical ventilators as vehicles of infection.twenty-five patients from the intensive care unit who had been subjected to tracheostomy and require mechanical respiration were studied. data are presented concerning the bacteriological controls performed on the tracheal exudates, various parts of the ventilators (engström model 200 and bennett pr-2), and the wright spirometer. the germs found were mainly pseudomonas, enterobacteriaceae germs and bacteria from the acinetobacter group. there was a high incidence of infection in the tracheostomi ...1975811072
in vitro susceptibility of acinetobacter calcoaceticus (herellea vagincola) to tetracycline and minocycline. 1976812659
the induction of mutants of acinetobacter calcoaceticus ncib8250 and their selection by vancomycin.the mutagenic and lethal effects of n-methyl-n'-nitro-n-nitrosoguanidine (ntg), ethyl methanesulphonate (ems), ultraviolet light irradiation and near-ultraviolet light irradiation with 8-methoxypsoralen on the bacterium acinetobacter calcoaceticus ncib8250 were examined. the production of auxotrophic mutants was used as a measure of mutagenic efficiency. under appropriate conditions all four agents were mutagenic. ems and ntg although more effective than irradiation, did not cause such a high fr ...1975812942
susceptibility of nonfermentative gram-negative bacilli to tobramycin.there has been increasing interest in the pathogenic role of nonfermentative gram-negative bacilli in human infections. except for pseudomonas aeruginosa, the susceptibility pattern of these organisms to tobramycin has not been evaluated thoroughly. the activity of tobramycin, as compared with that of gentamicin, was tested by the serial broth dilution technique against 178 isolates of nonfermentative gram-negative bacilli obtained from various sources. p. aeruginosa, pseudomonas stutzeri, acine ...1976823278
[infective situation in a cow barn contaminated with klebsiella mastitis].the infection situation in a four-row cow-house for 153 animals with a frequent occurrence of klebsiella mastitis was subject to a detailed analysis. the following results were obtained after two collections of blood and udder-quarter milk samples examined by the test-tube agglutination, gel precipitation, and bacteriological diagnosis methods: 1. streptococcus agalactiae was isolated from milk samples 28 and 31 times, pseudomonas aeruginosa 15 and 19 times, staphylococcus arueus 16 and 19 times ...1976828991
the microbiology of evacuated blood collection tubes.a collaborative study involving 20 clinical laboratories representing diverse geographical regions was organized to examine the microbiology of evacuated blooc collection tubes. overall, 14% of the 1433 tubes examined contained microorganisms representing a variety of bacteria and fungi. of note was the finding that nearly 9% of tubes with yellow closures, which are stated to be sterile by the manufacturers, contained bacteria, including pseudomonas aeruginosa, enterococci, serratia marcescens, ...1977835941
infections with acinetobacter calcoaceticus (herellea vaginicola): clinical and laboratory a retrospective review of 53 patients, 58 episodes of infection due to acinetobacter calcoaceticus var. anitratus (herellea vaginicola) were studied. although the organism is widely distributed in nature, it is of relatively low virulence since colonization is more frequently noted than infection and since most infections occur in patients subjected to the epidemiologic pressures common to nosocomial, gram-negative bacillary infection: prior antibiotic therapy; instrumentation and manipulatio ...1977846390
assimilatory nitrate reductase from acinetobacter calcoaceticus.a soluble nitrate reductase from the bacterium acinetobacter calcoaceticus grown on nitrate has been characterized. the reduction of nitrate to nitrite is mediated by an enzyme of 96000 molecular weight that can use as electron donors either viologen dyes chemically reduced with dithionite or enzymatically reduced with nad(p)h, through specific diaphorases which utilize viologens as electron acceptors. nitrate reductase activity is molybdenumdependent as shown by tungstate antagonistic experimen ...1977849099
[utilization of trimethylammonium-compounds by acinetobacter calcoaceticus (author's transl)].the utilization of carnitine and carnitine derivatives (o-acylcarnitines, carnitine carboxylderivatives) and structure-related trimethylammonium-compounds (betaines and nitrogen-bases) by acinetobacter calcoaceticus was studied by means of the control of growth and the quantitative detection of metabolites. the strain grew only on l-carnitine, l-o-acylcarnitines, and gamma-butyrobetaine as the sole carbon sources. the utilization of these compounds and the growth correlated with the cleavage of ...1977849100
[oxidation of n-tetradecan-1-14c by cell-free extracts from acinetobacter calcoaceticus]. 1977878503
in vitro synergism between carbenicillin and aminoglycosidic aminocyclitols against acinetobacter calcoaceticus var. anitratus.acinetobacter caleoaceticus var. anitratus is a nonfermentative, gram-negative bacillus that has been demonstrated to cause severe infections, usually in hospitalized patients. since mild to moderate resistance of a. calcoaceticus to one or more aminoglycosidic aminocyclitols has been noted to occur, a study was undertaken to evaluate the activity of combinations of carbenicillin with either kanamycin, tobramycin, or gentamicin against 28 isolates of a. calcoaceticus obtained from clinical sourc ...1977879749
sudden death due to waterhouse-friderichsen syndrome and purulent leptomeningitis caused by acinetobacter calcoaceticus (mima polymorpha).a case of waterhouse-friderichsen syndrome presenting with purulent leptomeningitis causing sudden death is described. the causative agent, acinetobacter calcoaceticus (mina polymorpha), was determined by post-mortem microbiological examination.1977883428
[induction of nadp+-dependent aldehyde dehydrogenase in acinetobacter calcoaceticus by hydrocarbons]. 1977888459
pseudobacteremia: false-positive blood cultures from mist tent the seven-month period from july 1975 through january 1976, 11 pediatric patients had acinetobacter calcoaceticus var. anitratus cultured from blood; this organism had not been isolated from pediatric patients in the previous six months. in 10 of 11 patients, only the first of two cultures was positive. all patients recovered uneventfully, although only two were treated with appropriate antibiotics. nine of 11 had been in mist tents at the time of the culture. mist cultured from one tent cont ...1977888818
[isolation and characterization of cell surface layers of acinetobacter calcoaceticus]. 1977930118
[polarographic determination of oxygen uptake of acinetobacter calcoaceticus during incubation with n-alkanes and their derivatives]. 1977930122
regulation of enzyme synthesis in the tryptophan pathway of acinetobacter acinetobacter calcoaceticus the seven genes coding for the enzymes responsible for tryptophan synthesis map at three chromosomal locations. two three-gene clusters, one (trpgdc) specifying the small subunit of anthranilate synthase, phosphoribosyl transferase, and indoleglycerol phosphate synthase and the other (trpfba) specifying phosphoribosyl anthranilate isomerase and both tryptophan synthase subunits, are not linked to each other or to the trpe gene specifying the large anthranilate synt ...1976931950
the fate of bacteria in frozen red cells.units of blood were intentionally contaiminated with suspensions of either aerobacter aerogenes, escherichia coli, bacillus subtilis, enterobacter cloacae, pseudomonas aeruginosa, sarcina lutea, seratia marcesens, staphylococcus epidermidis, streptococcus faecalis, paracolabacterum aerogenoides (enterobacter hafriae), mima polymorpha or acinetobacter- calcoaceticus. when inoculation was made prior to glycerolization, the subsequent glycerolization, freezing, thawing, and deglycerolization result ...1976936269
purification and properties of l-asparaginase b from acinetobacter calcoaceticus.l-asparaginase (l-asparagine amidohydrolase, ec b from acinetobacter calcoaceticus has been purified by precipitation with streptomycin, chromatography on deae-cellulose and cm-cellulose, gel filtration on agarose and chromatography on phosphocellulose. the molecular weight of the enzyme was found to be 130 000. the enzyme was rather insensitive to ph changes between 7 and 9. the michaelis constant was 3-10(-3) m. hg2+, cu2+, and ni2+ as well as high ionic strength inhibited the activit ...1976938683
[isolation and characterization of rubredoxin from acinetobacter calcoaceticus].acinetobacter calcoaceticus growing on long-chain n-alkanes contains a soluble iron-sulfur protein, which corresponds in its properties to a rubredoxin. it was prepared from the 50000 x g supernatant of ultrasonically treated cells using ion exchange chromatography on deae cellulose and gel filtration on sephadex g-75. the isolated protein is pure electrophoretically, but yields two bands corresponding to molecular weights of 6000 and 12000 respectively. a content of 11 acidic against 6 basic am ...1976970050
emergence of gentamicin-resistant bacteria: experience with tobramycin therapy of infections due to gentamicin-resistant organisms.a computerized system for testing and surveillance of bacterial susceptibility to antibiotics was used in monitoring the emergence of gentamicin-resistant strains of aerobic and facultative gram-negative bacilli at massachusetts general hospital since the release of gentamicin for clinical use in 1971. during the period studied, there was a significant increase in the prevalence of gentamicin-resistant bacteria, particularly among pseudomonas, acinetobacter (herellea), and proteus and, more rece ...1976972284
regulation of growth of acinetobacter calcoaceticus ncib8250 on benzyl alcohol in batch culture.formation of benzoate and catechol during oxidation of benzyl alcohol by washed suspensions of acinetobacter calcoaceticus ncib8250 confirmed earlier results indicating that this organism metabolizes benzyl alcohol via benzaldehyde, benzoate, and the 3-oxoadipate pathway. there was no evidence for feedback inhibition of benzyl alcohol dehydrogenase or benzaldehyde dehydrogenase ii. examination of growth curves and patterns of substrate utilization, as well as measurement of enzyme activities, sh ...1976993779
mandelate dehydrogenase with altered stereospecificity in mutant strains of acinetobacter calcoaceticus n.c.i.b. 8250. 19761001725
constitutivity of the mandelate enzymes in acinetobacter calcoaceticus, n.c.i.b. 8250, and its effect on the synthesis of benzyl alcohol dehydrogenase. 19761022571
rp4 fertility variants in acinetobacter calcoaceticus. 19761026570
common enterobacterial antigen in yersinia enterocolitica.production of the common enterobacterial antigen (ca) by strains of yersinia enterocolitica (y.e.) serotypes 3 and 9 (winblad), by strains of 5 different y.e. serogroups (wauters) and various other bacteria was examined. antibody against ca was raised by immunization of rabbits with e. coli o 14. extract prepared from s. typhimurium was used for the sensitization of sheep erythrocytes with ca. absorption and haemagglutination inhibition experiments showed that ca could be detected in heat extrac ...19751098394
activity of minocycline against acinetobacter calcoaceticus var. anitratus (syn. herellea vaginicola) and serratia marcescens.the activity of minocycline and tetracycline against 23 isolates of acinetobacter calcoaceticus var. anitratus (syn. herellea vaginicola) and 178 strains of serratia marcescens was determined by disk and microdilution methods. the results indicate that minocycline is highly active against this species of acinetobacter, all but one strain being inhibited by 0.007 mug of the antibiotic per ml. tetracycline was also active, though to a lesser degree, against a. calcoaceticus. of the 178 strains of ...19751099982
correlation between twitching motility and possession of polar fimbriae in acinetobacter calcoaceticus.negatively stained preparations of 16 strains of acinetobacter calcoaceticus were examined in the electron microscope. five of the strains did and 11 of the strains did not exhibit twitching motility. two of the non-twitching strains were substrains of twitching wild-type strains. all twitching strains were found to possess fimbriae with a diameter of approximately 50 a. these fimbriae were shown to be of polar origin except in one strain where, for technical reasons, the origin could not be det ...19751155109
the occurrence of twitching motility among gram-negative bacteria.almost 1000 strains representing well above 50 different species or groups of gram-negative bacteria were examined for twitching motility. this kind of motility was mainly found in strictly aerobic cocci and rods (viz. acinetobacter calcoaceticus, moraxella spp., neisseria gonorrhoeae, n. meningitidis, pseudomonas spp., phenon 3 of thornley that is closely related to acinetobacter, and marine, yellow-pigmented rods), but also in the facultative eikenella corrodens and in anaerobic strains presum ...19751155115
beta-ketoadipate enol-lactone hydrolases i and ii from acinetobacter calcoaceticus.beta-ketoadipate enol-lactone hydrolase catalyzes a common step in the utilization of protocatechuate and cis,cis-muconate by bacteria. either of the two compounds elicits the synthesize of an enol-lactone hydrolase in acinetobacter. the enol-lactone hydrolase that is induced by each compound was purified, and the properties of the proteins were compared. both enzymes appear to be dimers with molecular weights of approximately 25,000. the amino acid compositions of the enzymes differ, and the tw ...19751158871
metabolism of monofluorobenzoates by acinetobacter calcoaceticus n.c.i.b. 8250. formation of monofluorocatechols.none of the monofluorobenzoates serves as sole source of carbon and energy for growth of acinetobacter calcoaceticus but all can contribute to growth on other substrates. the monofluorobenzoates are oxidised by bacteria pre-induced for benzoate oxidation and can themselves induce the appropriate enzymes. the initial products of oxidation have been separated and identified by gas-liquid chromatography. 2-flurobenzoate is oxidised to catechol, fluoride and 3-fluorocatechol; 3-fluorobenzoate gives ...19751182155
[inhibiton of isocitrate dehydrogenase and isocitrate lyase from acinetobacter calcoaceticus by acids of the citrate and glyoxylate cycle].acinetobacter calcoaceticus contains two forms of nadp+-dependent isocitrate dehydrogenases differing, among others, by their molecular weights and regulatory properties. the regulation of the high-molecular form of isocitrate dehydrogenase and of isocitrate lyase by organic acids, either belonging or related to the citrate and glyoxalate cycle, is investigated. while alpha-ketoglutarate and oxalacetate competitively inhibit the isocitrate dehydrogenase against ds-isocitrate, glyoxylate and pyru ...19751199592
regulation of growth of acinetobacter calcoaceticus ncib8250 on l-mandelate in batch culture.batch culture of acinetobacter calcoaceticus in l-mandelate- or phenylglyoxylate-salts medium showed an unusual non-exponential pattern unless the inoculum had been grown on benzyl alcohol. there were transient accumulations of benzaldehyde and benzyl alcohol caused by the limitation of l-mandelate oxidation by low activities of benzaldehyde dehydrogenase and the diversion of reducing power to the formation of benzyl alcohol. in vivo enzymic activities were estimated from patterns of substrate u ...19751206374
[effect on growing conditions on protein content and amino acid composition of acinetobacter calcoaticus].the protein content and amino acid composition of acinetobacter calcoaceticus were studied as dependent on the stages of development and different hydrocarbon sources. the protein content was always the greatest at the logarithmic stage and decreased approaching the stationary stage. when cultivating on different hyrocarbon sources only an insignificant differedce in the content of protein was observed. a relative amino acid composition of ac. calcoaceticus remained practically constant during t ...19751209770
protocatechuate 3, 4-dioxygenase from acinetobacter calcoaceticus.protocatechuate 3,4-dioxygenase (pcd) from p-hydroxybenzoate-induced cells of acinetobacter calcoaceticus was purified by heat and protamine sulfate treatment, ammonium sulfate fractionation, deae-cellulose, and sephadex g-200 column chromatography. the enzyme appears to be homogeneous by ultracentrifugation and acrylamide gel electrophoresis. this is the first report of pcd purified from acinetobacter. for comparison, crystalline pseudomonas pcd was also obtained. the enzymes from acinetobacter ...19761252412
leprosy in hiv-positive and syphilitic young paraguayan a borderline lepromatous patient a positive serology for syphilis and hiv 1 has been detected. the patient also had an urinary tract infection with acinetobacter calcoaceticus. in paraguay up till now no leprosy cases infected with hiv have been reported.19921293911
a simultaneous bilateral attack of infectious corneal ulcers in an extended-wear soft contact lens wearer: a case report.a case study was made of a 23-year-old female with simultaneous bilateral infectious corneal ulcers related to extended-wear soft contact lenses. cultures revealed pseudomonas aeruginosa and acinetobacter calcoaceticus. multiple high risk factors were combined, including extended-wear, poor lens care, bilateral alternate wearing, and smoking. amphetamine addiction may also have played a role. after treatment, a visual acuity of 20/20 was achieved in both eyes by the use of rigid gas-permeable co ...19921296043
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