| problems associated with identification of legionella species from the environment and isolation of six possible new species. | following investigation of an outbreak of legionellosis in south australia, numerous legionella-like organisms were isolated from water samples. because of the limited number of commercially available direct fluorescent-antibody reagents and the cross-reactions found with some reagents, non-pneumophila legionellae proved to be difficult to identify and these isolates were stored at -70 degrees c for later study. latex agglutination reagents for legionella pneumophila and legionella anisa develop ... | 1990 | 2317047 |
| development and evaluation of chlamylege, a new commercial test allowing simultaneous detection and identification of legionella, chlamydophila pneumoniae, and mycoplasma pneumoniae in clinical respiratory specimens by multiplex pcr. | this study describes the development and evaluation of a new commercial test, chlamylege (argene inc.), which allows the simultaneous detection in respiratory samples of chlamydophila pneumoniae, mycoplasma pneumoniae, and most legionella species, as well as pcr inhibitors, by using a multiplex pcr and microplate hybridization. the sensitivities of chlamylege were 1 x 10(-3) ifu, 5 x 10(-2) color-changing units, and 1 cfu per reaction tube for c. pneumoniae, m. pneumoniae, and legionella pneumop ... | 2005 | 16000443 |
| rapid method for enumeration of viable legionella pneumophila and other legionella spp. in water. | a sensitive and specific method has been developed to enumerate viable l. pneumophila and other legionella spp. in water by epifluorescence microscopy in a short period of time (a few hours). this method allows the quantification of l. pneumophila or other legionella spp. as well as the discrimination between viable and nonviable legionella. it simultaneously combines the specific detection of legionella cells using antibodies and a bacterial viability marker (chemchrome v6), the enumeration bei ... | 2005 | 16000824 |
| legionella confirmation using real-time pcr and syto9 is an alternative to current methodology. | the currently accepted culture techniques for the detection of legionella spp. in water samples (as/nzs 3896:1998 and iso 11731 standard methods) are slow and laborious, requiring from 7 to 14 days for a result. we describe a fully validated rapid confirmation technique that uses real-time pcr incorporating the intercalating dye syto9 for the direct identification of primary cultures, significantly decreasing turnaround time and allowing faster remedial action to be taken by the industry. | 2005 | 16332896 |
| two legionnaires' disease cases associated with industrial waste water treatment plants: a case report. | finnish and swedish waste water systems used by the forest industry were found to be exceptionally heavily contaminated with legionellae in 2005. | 2010 | 21126333 |
| a side by side comparison of bruker biotyper and vitek ms: utility of maldi-tof ms technology for microorganism identification in a public health reference laboratory. | matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) has emerged as a rapid, highly accurate, and cost-effective method for routine identification of a wide range of microorganisms. we carried out a side by side comparative evaluation of the performance of bruker biotyper versus vitek ms for identification of a large and diverse collection of microorganisms. most difficult and/or unusual microorganisms, as well as commonly encountered microorganisms were se ... | 2015 | 26658918 |
| genome dynamics in legionella: the basis of versatility and adaptation to intracellular replication. | legionella pneumophila is a bacterial pathogen present in aquatic environments that can cause a severe pneumonia called legionnaires' disease. soon after its recognition, it was shown that legionella replicates inside amoeba, suggesting that bacteria replicating in environmental protozoa are able to exploit conserved signaling pathways in human phagocytic cells. comparative, evolutionary, and functional genomics suggests that the legionella-amoeba interaction has shaped this pathogen more than p ... | 2013 | 23732852 |
| free-living amoebae and their associated bacteria in austrian cooling towers: a 1-year routine screening. | free-living amoebae (fla) are widely spread in the environment and known to cause rare but often serious infections. besides this, fla may serve as vehicles for bacterial pathogens. in particular, legionella pneumophila is known to replicate within fla thereby also gaining enhanced infectivity. cooling towers have been the source of outbreaks of legionnaires' disease in the past and are thus usually screened for legionellae on a routine basis, not considering, however, fla and their vehicle func ... | 2016 | 27177720 |
| identification of early diagnostic antigens from major excretory-secretory proteins of trichinella spiralis muscle larvae using immunoproteomics. | the excretory-secretory (es) proteins of trichinella spiralis muscle larvae (ml) come mainly from the excretory granules of the stichosome and the cuticles (membrane proteins), are directly exposed to the host's immune system, and are the main target antigens, which induce the immune responses. although the es proteins are the most commonly used diagnostic antigens for trichinellosis, their main disadvantage are the false negative results during the early stage of infection. the aim of this stud ... | 2014 | 24450759 |