| the reca protein as a model molecule for molecular systematic studies of bacteria: comparison of trees of recas and 16s rrnas from the same species. | the evolution of the reca protein was analyzed using molecular phylogenetic techniques. phylogenetic trees of all currently available complete reca proteins were inferred using multiple maximum parsimony and distance matrix methods. comparison and analysis of the trees reveal that the inferred relationships among these proteins are highly robust. the reca trees show consistent subdivisions corresponding to many of the major bacterial groups found in trees of other molecules including the alpha, ... | 1995 | 8587109 |
| genetic and biochemical characterization of the pathway in pantoea citrea leading to pink disease of pineapple. | pink disease of pineapple, caused by pantoea citrea, is characterized by a dark coloration on fruit slices after autoclaving. this coloration is initiated by the oxidation of glucose to gluconate, which is followed by further oxidation of gluconate to as yet unknown chromogenic compounds. to elucidate the biochemical pathway leading to pink disease, we generated six coloration-defective mutants of p. citrea that were still able to oxidize glucose into gluconate. three mutants were found to be af ... | 2000 | 10735866 |
| catalytic and molecular properties of the quinohemoprotein tetrahydrofurfuryl alcohol dehydrogenase from ralstonia eutropha strain bo. | the quinohemoprotein tetrahydrofurfuryl alcohol dehydrogenase (thfa-dh) from ralstonia eutropha strain bo was investigated for its catalytic properties. the apparent k(cat)/k(m) and k(i) values for several substrates were determined using ferricyanide as an artificial electron acceptor. the highest catalytic efficiency was obtained with n-pentanol exhibiting a k(cat)/k(m) value of 788 x 10(4) m(-1) s(-1). the enzyme showed substrate inhibition kinetics for most of the alcohols and aldehydes inve ... | 2001 | 11222593 |
| cloning of the membrane-bound aldehyde dehydrogenase gene of acetobacter polyoxogenes and improvement of acetic acid production by use of the cloned gene. | a genomic clone bank of acetobacter polyoxogenes nbi1028 constructed in escherichia coli by use of the expression vector puc18 was screened with antibody raised against membrane-bound aldehyde dehydrogenase (aldh; 75 kilodaltons [kda]) from a. polyoxogenes nbi1028. a clone that synthesized a 41-kda protein cross-reactive with anti-aldh antibody was isolated. for cloning of the full-length aldh structural gene, a cosmid gene bank was screened by southern blot hybridization with the cloned dna as ... | 1989 | 16347820 |
| putative abc transporter responsible for acetic acid resistance in acetobacter aceti. | two-dimensional gel electrophoretic analysis of the membrane fraction of acetobacter aceti revealed the presence of several proteins that were produced in response to acetic acid. a 60-kda protein, named aata, which was mostly induced by acetic acid, was prepared; aata was cloned on the basis of its nh2-terminal amino acid sequence. aata, consisting of 591 amino acids and containing atp-binding cassette (abc) sequences and abc signature sequences, belonged to the abc transporter superfamily. the ... | 2006 | 16391084 |
| control of acetic acid fermentation by quorum sensing via n-acylhomoserine lactones in gluconacetobacter intermedius. | a number of gram-negative bacteria regulate gene expression in a cell density-dependent manner by quorum sensing via n-acylhomoserine lactones (ahls). gluconacetobacter intermedius nci1051, a gram-negative acetic acid bacterium, produces three different ahls, n-decanoyl-l-homoserine lactone, n-dodecanoyl-l-homoserine lactone, and an n-dodecanoyl-l-homoserine lactone with a single unsaturated bond in its acyl chain, as determined by liquid chromatography-tandem mass spectrometry. two genes encodi ... | 2008 | 18245283 |
| an ompa family protein, a target of the gini/ginr quorum-sensing system in gluconacetobacter intermedius, controls acetic acid fermentation. | via n-acylhomoserine lactones, the gini/ginr quorum-sensing system in gluconacetobacter intermedius nci1051, a gram-negative acetic acid bacterium, represses acetic acid and gluconic acid fermentation. two-dimensional polyacrylamide gel electrophoretic analysis of protein profiles of strain nci1051 and gini and ginr mutants identified a protein that was produced in response to the gini/ginr regulatory system. cloning and nucleotide sequencing of the gene encoding this protein revealed that it en ... | 2008 | 18487322 |
| a rationally designed peptide enhances homologous recombination in vitro and resistance to dna damaging agents in vivo. | the reca family of proteins is essential in homologous recombination, a critical step in dna repair. here, we report that a rationally-designed small peptide based on the crystal structure of escherichia coli reca-dna complex can promote homologous recombination through the enhancement of both reca-mediated strand assimilation and three-strand exchange activity. among 17 peptides tested, peptide #3 with the amino acid sequence of irfltarrr has the most potent activity in promoting the reca-media ... | 2010 | 20308162 |
| molecular and catalytic properties of the aldehyde dehydrogenase of gluconacetobacter diazotrophicus, a quinoheme protein containing pyrroloquinoline quinone, cytochrome b, and cytochrome c. | several aldehyde dehydrogenase (aldh) complexes have been purified from the membranes of acetic acid bacteria. the enzyme structures and the chemical nature of the prosthetic groups associated with these enzymes remain a matter of debate. we report here on the molecular and catalytic properties of the membrane-bound aldh complex of the diazotrophic bacterium gluconacetobacter diazotrophicus. the purified aldh complex is a heterodimer comprising two subunits of 79.7 and 50 kda, respectively. reve ... | 2010 | 20802042 |
| recombinant organisms for production of industrial products. | a revolution in industrial microbiology was sparked by the discoveries of ther double-stranded structure of dna and the development of recombinant dna technology. traditional industrial microbiology was merged with molecular biology to yield improved recombinant processes for the industrial production of primary and secondary metabolites, protein biopharmaceuticals and industrial enzymes. novel genetic techniques such as metabolic engineering, combinatorial biosynthesis and molecular breeding te ... | 2009 | 21326937 |
| recombinant organisms for production of industrial products. | a revolution in industrial microbiology was sparked by the discoveries of ther double-stranded structure of dna and the development of recombinant dna technology. traditional industrial microbiology was merged with molecular biology to yield improved recombinant processes for the industrial production of primary and secondary metabolites, protein biopharmaceuticals and industrial enzymes. novel genetic techniques such as metabolic engineering, combinatorial biosynthesis and molecular breeding te ... | 2009 | 21326937 |
| the oxidative fermentation of ethanol in gluconacetobacter diazotrophicus is a two-step pathway catalyzed by a single enzyme: alcohol-aldehyde dehydrogenase (adha). | gluconacetobacter diazotrophicus is a n2-fixing bacterium endophyte from sugar cane. the oxidation of ethanol to acetic acid of this organism takes place in the periplasmic space, and this reaction is catalyzed by two membrane-bound enzymes complexes: the alcohol dehydrogenase (adh) and the aldehyde dehydrogenase (aldh). we present strong evidence showing that the well-known membrane-bound alcohol dehydrogenase (adha) of ga. diazotrophicus is indeed a double function enzyme, which is able to use ... | 2015 | 25574602 |