| methanol and ethanol oxidase respiratory chains of the methylotrophic acetic acid bacterium, acetobacter methanolicus. | acetobacter methanolicus is a unique acetic acid bacterium which has a methanol oxidase respiratory chain, as seen in methylotrophs, in addition to its ethanol oxidase respiratory chain. in this study, the relationship between methanol and ethanol oxidase respiratory chains was investigated. the organism is able to grow by oxidizing several carbon sources, including methanol, glycerol, and glucose. cells grown on methanol exhibited a high methanol-oxidizing activity and contained large amounts o ... | 1992 | 1323563 |
| expression of the core antigen gene of hepatitis b virus (hbv) in acetobacter methanolicus using broad-host-range vectors. | using the broad-host-range promoter probe vector prs201 for cloning of phage acm1 promoters, we established a convenient vector system for expression of heterologous genes in different gram-negative bacteria. the usefulness of this system was demonstrated by expression of the hbv core gene in acetobacter methanolicus. plasmids carrying the hbv core gene downstream of different acm1-phage promoters were transferred to a. methanolicus, a new potential host for recombinant dna expression. using enz ... | 1991 | 1367579 |
| the interaction of methanol dehydrogenase and cytochrome cl in the acidophilic methylotroph acetobacter methanolicus. | the quinoprotein methanol dehydrogenase (mdh) of acetobacter methanolicus has an alpha 2 beta 2 structure. by contrast with other mdhs, the beta-subunit (approx. 8.5 kda) does not contain the five lysine residues previously proposed to be involved in ionic interactions with the electron acceptor cytochrome cl. that electrostatic interactions are involved was confirmed by the demonstration that methanol:cytochrome cl oxidoreductase activity was inhibited by high ionic strength (i), the strength o ... | 1991 | 1660263 |
| structure of the extracellular polysaccharide of acetobacter methanolicus mb 58/4 (imet 10945). | | 1991 | 1802387 |
| structure of the capsular polysaccharide and the o-side-chain of the lipopolysaccharide from acetobacter methanolicus mb 58/4 (imet 10945), and of oligosaccharides resulting from their degradation by the bacteriophage acml. | the capsular polysaccharide (cps) and the o-side-chain of the lipopolysaccharide (lps) of acetobacter methanolicus mb 58/4 (imet 10945) have been shown to contain the same disaccharide repeating unit, namely, ----2)-beta-d-galf-(1----3)-beta-d-galp-(1----. degradation of the cps and the lps with the bacteriophage acml gave fragments built up of 1-5 repeating units; the octasaccharide preponderated. the phage-associated depolymerase proved to be a beta-d-galactofuranoside hydrolase. | 1991 | 1811855 |
| mutagenesis of acetobacter methanolicus mb58 with the transposon tn5. | transposon mutagenesis was applied to the isolation of mutants of the facultatively methylotrophic acetobacter methanolicus mb 58. the transposon tn5 (psu2011) was transferred from escherichia coli sm 10 by means of conjugation to acetobacter methanolicus mb 58. four out of 1850 stable km-resistant transconjugants were identified that were formaldehyde sensitive and failed to grow on methanol. | 1990 | 2166787 |
| 3-hexulose-6-phosphate synthase from acetobacter methanolicus mb58. | | 1990 | 2280713 |
| detoxification of formaldehyde by acetic acid bacteria. | formaldehyde resistance of methylotrophic and non-methylotrophic acetobacter strains was investigated. a facultatively methylotrophic acetobacter methanolicus (mb58) gets rid of free formaldehyde by assimilating it. heterotrophically growing cells tolerate 12 mm free formaldehyde. non-methylotrophic but methanol oxidizing acetobacter pasteurianus strains possess the same level of formaldehyde resistance. formaldehyde resistance can be drastically lowered down to 4 mm by blocking the formate dehy ... | 1989 | 2775425 |
| [acetobacter methanolicus--a new organism for genetic studies]. | a new bacterial strain is described belonging to acetobacter methanolicus species. it is of industrial value as a producer of protein and methanol products. the strain is acidophile and this feature comprises a conspicuous technological advantage. the results of bacteriophage and cell interactions are reported. they might be potentially useful for elaboration of the transduction technique for the strain. the collection of mutants was obtained including those utilizing methanol, having auxotrophi ... | 1985 | 3842739 |
| structure of the capsular polysaccharide and the o-side-chain of the lipopolysaccharide from acetobacter methanolicus mb 70, and of oligosaccharides resulting from their degradation by the bacteriophage acm6. | acetobacter methanolicus mb 70 was shown to be related to the type strain of this species mb 58/4 (imet 10945) having the same galactan-->2)-beta-d-gal f-(1-->3)-beta-d-gal p-(1-->as the capsular polysaccharide (cps) and the o-side-chain of the lipopolysaccharide (lps). additionally, a glucan built up of the disaccharide repeating unit-->6)-alpha-d-glc p-(1-->2)-alpha-d-glc p-(1-->was identified in strain mb 70. in the cps, the polymers were present in the ratio approximately 1:1, whereas the gl ... | 1994 | 7512445 |
| structure of the capsular polysaccharide and the o-side-chain of the lipopolysaccharide from acetobacter methanolicus mb 135 (imet 11402). | | 1994 | 7512446 |
| repression of lipopolysaccharide biosynthesis in escherichia coli by an antisense rna of acetobacter methanolicus phage acm1. | lysogenic acetobacter methanolicus strains carrying the prophage acm1 were found to be unable to synthesize both the capsular polysaccharide (cps) and the o-specific side-chain of lipopolysaccharide (lps) and to represent rough variants of the host bacterium. a 262 bp dna fragment of phage acm1, obviously required for interference with lps biosynthesis, was cloned and expressed in escherichia coli. independently of the o-type, transformation of various e. coli strains with the recombinant dna re ... | 1995 | 7542725 |
| growth phase-dependent induction of stationary-phase promoters of escherichia coli in different gram-negative bacteria. | rsf1010-derived plasmids carrying a fusion of a promoterless lacz gene with the sigma s-dependent growth phase-regulated promoters of escherichia coli, bolap1 and fic, were constructed. the plasmids were mobilized into the gram-negative bacterial species acetobacter methanolicus, xanthomonas campestris, pseudomonas putida, and rhizobium meliloti. the beta-galactosidase activities of bacterial cultures were determined during exponential and stationary growth phases. transcriptional activation of ... | 1995 | 7665531 |
| structure of the acetobacter methanolicus mb 129 capsular polysaccharide, and of oligosaccharides resulting from degradation by bacteriophage acm7. | the capsular polysaccharide of acetobacter methanolicus mb 129 consists of d-glc, d-gal, l-rha, and l-glyceric acid in the molar ratios 1:1:1:0.3. periodate oxidation, methylation analysis, solvolysis with hf, and detailed 1h and 13c nmr analysis resulted in the structure of the repeating unit shown below. [formula: see text] bacteriophage acm7-associated end-alpha-l-rhamnopyranoside hydrolase depolymerizes the cps even in the presence of the o-acyl group, to give the respective hexa-, nona-, an ... | 1994 | 8039190 |
| structure of the capsular polysaccharide and the o-side-chain of the lipopolysaccharide from acetobacter methanolicus mb 58 (imet b346). | | 1994 | 8137368 |
| phage acm1-mediated transduction in the facultatively methanol-utilizing acetobacter methanolicus mb 58/4. | phage acm1, generally virulent for the acidophilic facultatively methanol-utilizing strain of acetobacter methanolicus mb 58/4, is also capable of lysogenizing its host strain at a low rate. using amino acid-auxotrophic mutants of a. methanolicus mb 58/4 as recipient strains, transduction of his, leu and tyr markers could be demonstrated in this system. the ability to prepare transducing lysates by propagation of phage acm1 on the prototrophic donor strain a. methanolicus mb 58/4, the transducti ... | 1993 | 8376955 |
| a novel quinoprotein methanol dehydrogenase containing an additional 32-kilodalton peptide purified from acetobacter methanolicus: identification of the peptide as a moxj product. | acetobacter methanolicus is a unique acetic acid bacterium which has a methanol oxidase respiratory chain in addition to an ethanol oxidase respiratory chain. in this study, two different forms of methanol dehydrogenase (type i and ii mdhs) were purified from a. methanolicus grown on methanol. type i mdh was more basic (pi of 8.0) and smaller (m(r) of 148k) than type ii mdh (pi of 6.7 and m(r) of 177k). type i mdh consisted of alpha and beta subunits of 62 and 10 kda, which has the same alpha 2 ... | 1993 | 8389187 |
| the toxicity of substituted phenolic compounds to a detoxifying and an acetic acid bacterium. | in the detoxifying bacterium acinetobacter calcoaceticus 69-v and in the acetic acid bacterium acetobacter methanolicus mb 58, glucose and xylose are oxidized, respectively, via pqq-dependent membrane-bound dehydrogenases, which are linked to the respiratory chain in a manner enabling energy conservation via electron transport phosphorylation (etp) in the cytoplasmic membrane. neither the glucose and gluconic acid nor the xylose and xylonic acid are metabolized. therefore, measurements of sugar ... | 1997 | 9143455 |
| controlled secretion into the culture medium of a hybrid beta-glucanase by acetobacter methanolicus mediated by the kil gene of escherichia coli located on a tn5-derived transposon. | a tn5-based transposon bearing the kil gene (killing protein), mediating controlled export of periplasmic proteins into the culture medium, was constructed (tn5-kil3). this transposon contained the kil gene of the colel plasmid under the growth-phase-dependent promoter of the fic gene (filamentation induced by camp) of escherichia coli, an interposon located upstream of kil, a kanamycin/neomycin-resistance gene, a multiple cloning site and the mob site. the transposition of tn5-kil3 to acetobact ... | 1997 | 9210342 |
| the phylogeny of acetic acid bacteria based on the partial sequences of 16s ribosomal rna: the elevation of the subgenus gluconoacetobacter to the generic level. | thirty-six strains of acetic acid bacteria classified in the genera acetobacter, gluconobacter, and acidomonas were examined for their partial base sequences in positions 1220 through 1375, 156 bases, of 16s rrna. the strains of the q10-equipped gluconobacter species examined were divided into two subgroups, which included the type strains of gluconobacter oxydans, the type species of the genus gluconobacter, and of a second species, gluconobacter cerinus, respectively. the base differences numb ... | 1997 | 9301103 |
| intramolecular electron transport in quinoprotein alcohol dehydrogenase of acetobacter methanolicus: a redox-titration study | quinohemoprotein-cytochrome c complex alcohol dehydrogenase (adh) of acetic acid bacteria consists of three subunits, of which subunit i contains pyrroloquinoline quinone (pqq) and heme c, and subunit ii contains three heme c components. the pqq and heme c components are believed to be involved in the intramolecular electron transfer from ethanol to ubiquinone. to study the intramolecular electron transfer in adh of acetobacter methanolicus, the redox potentials of heme c components were determi ... | 1998 | 9526036 |
| constitutive and inducible green fluorescent protein expression in bartonella henselae. | the green fluorescent protein (gfp) gene was expressed on a plasmid in b. henselae, and gfp-expressing bacteria were visualized by fluorescence microscopy. hep-2 cells infected with gfp-expressing bacteria were separated from uninfected cells with a fluorescence activated cell sorter. promoter fusions of b. henselae chromosomal dna to gfp were examined by flow cytometry, and a b. henselae groel promoter fusion which induced expression at 37 degreesc was isolated. | 1998 | 9673287 |
| distribution of tetrahydromethanopterin-dependent enzymes in methylotrophic bacteria and phylogeny of methenyl tetrahydromethanopterin cyclohydrolases. | the methylotrophic proteobacterium methylobacterium extorquens am1 possesses tetrahydromethanopterin (h(4)mpt)-dependent enzymes, which are otherwise specific to methanogenic and sulfate-reducing archaea and which have been suggested to be involved in formaldehyde oxidation to co(2) in m. extorquens am1. the distribution of h(4)mpt-dependent enzyme activities in cell extracts of methylotrophic bacteria from 13 different genera are reported. h(4)mpt-dependent activities were detected in all of th ... | 1999 | 10482517 |
| the respiratory system and diazotrophic activity of acetobacter diazotrophicus pal5. | the characteristics of the respiratory system of acetobacter diazotrophicus pal5 were investigated. increasing aeration (from 0.5 to 4.0 liters of air min(-1) liter of medium(-1)) had a strong positive effect on growth and on the diazotrophic activity of cultures. cells obtained from well-aerated and diazotrophically active cultures possessed a highly active, membrane-bound electron transport system with dehydrogenases for nadh, glucose, and acetaldehyde as the main electron donors. ethanol, suc ... | 1999 | 10559164 |
| microbial ecology of an extreme acidic environment, the tinto river. | the tinto river (huelva, southwestern spain) is an extreme environment with a rather constant acidic ph along the entire river and a high concentration of heavy metals. the extreme conditions of the tinto ecosystem are generated by the metabolic activity of chemolithotrophic microorganisms thriving in the rich complex sulfides of the iberian pyrite belt. molecular ecology techniques were used to analyze the diversity of this microbial community. the community's composition was studied by denatur ... | 2003 | 12902280 |
| emendation of the genus acidomonas urakami, tamaoka, suzuki and komagata 1989. | the genus acidomonas and the species acidomonas methanolica were recharacterized by using the type strain (nric 0498(t)), three reference strains and 10 methanol-utilizing bacteria that were isolated from activated sludge from three different sewage-treatment plants in tokyo. based on 16s rdna sequences, all strains formed a single cluster within the acetobacteraceae that was clearly different from the genera acetobacter, gluconobacter, gluconacetobacter, asaia and kozakia: the 14 strains were i ... | 2004 | 15143037 |
| diversity and functional analysis of bacterial communities associated with natural hydrocarbon seeps in acidic soils at rainbow springs, yellowstone national park. | in this paper we describe the bacterial communities associated with natural hydrocarbon seeps in nonthermal soils at rainbow springs, yellowstone national park. soil chemical analysis revealed high sulfate concentrations and low ph values (ph 2.8 to 3.8), which are characteristic of acid-sulfate geothermal activity. the hydrocarbon composition of the seep soils consisted almost entirely of saturated, acyclic alkanes (e.g., n-alkanes with chain lengths of c15 to c30, as well as branched alkanes, ... | 2005 | 16204508 |
| formaldehyde uptake by methylobacterium sp. mf1 and acidomonas methanolica mb 58 with the different formaldehyde assimilation pathways. | methylobacterium sp. mf1 (an obligate methylotrophic bacterium isolated newly by the authors) and acidomonas methanolica mb58 (a facultative methylotrophic bacterium) uptake formaldehyde similarly. it was found that the former assimilated formaldehyde via the serine pathway whereas the latter did so via the ribulose-monophosphate pathway from the measurement of the key enzyme activities in each assimilation pathway. that is, hydroxy pyruvate reductase was detected in only the above-mentioned mf1 ... | 2006 | 17095990 |
| tanticharoenia sakaeratensis gen. nov., sp. nov., a new osmotolerant acetic acid bacterium in the alpha-proteobacteria. | tanticharoenia sakaeratensis gen. nov., sp. nov. is proposed for three strains isolated from soil collected in thailand. the three strains, ac37(t), ac38, and ac39, were included within a lineage comprising the genera asaia, kozakia, swaminathania, neoasaia, acetobacter, gluconobacter, and saccharibacter in a phylogenetic tree based on 16s rrna gene sequences, but formed a quite different, independent cluster. pair-wise sequence similarities of strain ac37(t) were 96.5-92.1% to the type strains ... | 2008 | 18323667 |
| mxaf gene, a gene encoding alpha subunit of methanol dehydrogenase in and false growth of acetic acid bacteria on methanol. | mxaf gene, a gene encoding alpha subunit of methanol dehydrogenase, was investigated for acetic acid bacteria, and growth on methanol was examined for the bacteria by using various media. of 21 strains of acetic acid bacteria studied, acidomonas methanolica strains showed the presence of mxaf gene exclusively, and grew on a defined medium containing methanol. further, none of the strains tested of which the growth on methanol had been previously reported, except for acidomonas methanolica, showe ... | 2009 | 19436127 |
| magnetospirillum bellicus sp. nov., a novel dissimilatory perchlorate-reducing alphaproteobacterium isolated from a bioelectrical reactor. | previously isolated dissimilatory perchlorate-reducing bacteria (dprb) have been primarily affiliated with the betaproteobacteria. enrichments from the cathodic chamber of a bioelectrical reactor (ber) inoculated from creek water in berkeley, ca, yielded a novel organism most closely related to a previously described strain, wd (99% 16s rrna gene identity). strain vdy(t) has 96% 16s rrna gene identity to both magnetospirillum gryphiswaldense and magnetospirillum magnetotacticum, and along with s ... | 2010 | 20495050 |
| purification, crystallization and preliminary x-ray crystallographic analysis of a methanol dehydrogenase from the marine bacterium methylophaga aminisulfidivorans mp(t). | methylophaga aminisulfidivorans mp(t) is a marine methylotrophic bacterium that utilizes c(1) compounds such as methanol as a carbon and energy source. the released electron from oxidation flows through a methanol-oxidizing system (mox) consisting of a series of electron-transfer proteins encoded by the mox operon. one of the key enzymes in the pathway is methanol dehydrogenase (mdh), which contains the prosthetic group pyrroloquinoline quinone (pqq) and converts methanol to formaldehyde in the ... | 2011 | 21505255 |
| optimization of culture conditions to produce high yields of active acetobacter sp. cctcc m209061 cells for anti-prelog reduction of prochiral ketones. | chiral alcohols are widely used in the synthesis of chiral pharmaceuticals, flavors and functional materials and appropriate whole-cell biocatalysts offer a highly enantioselective, minimally polluting route to these valuable compounds. the recently isolated strain acetobacter sp. cctcc m209061 showed exclusive anti-prelog stereoselectivity for the reduction of prochiral ketones, but the low biomass has limited its commercialization and industrial applications. to tackle this problem, the effect ... | 2011 | 22099947 |
| crystallization and preliminary x-ray crystallographic analysis of mxaj, a component of the methanol-oxidizing system operon from the marine bacterium methylophaga aminisulfidivorans mpt. | the methanol-oxidizing system (mox) is essential for methylotrophic bacteria to extract energy during the oxidoreduction reaction and consists of a series of electron-transfer proteins encoded by the mox operon. one of the key enzymes is the α₂β₂ methanol dehydrogenase complex (type i mdh), which converts methanol to formaldehyde during the 2e⁻ transfer through the prosthetic group pyrroloquinoline quinone. mxaj, a product of mxaj of the mox operon, is a component of the mdh complex and enhances ... | 2013 | 23908039 |
| simultaneous host-pathogen transcriptome analysis during granulibacter bethesdensis infection of neutrophils from healthy subjects and patients with chronic granulomatous disease. | polymorphonuclear leukocytes (pmn) from patients with chronic granulomatous disease (cgd) fail to produce microbicidal concentrations of reactive oxygen species (ros) due to mutations in nox2. patients with cgd suffer from severe, life-threatening infections and inflammatory complications. granulibacter bethesdensis is an emerging gram-negative pathogen in cgd that resists killing by pmn of cgd patients (cgd pmn) and inhibits pmn apoptosis through unknown mechanisms. microarray analysis was used ... | 2015 | 26283340 |
| acidomonas methanolica-associated necrotizing lymphadenitis in a patient with chronic granulomatous disease. | adenitis for which no causative organism can be isolated is a common occurrence in patients with chronic granulomatous disease (cgd). here we identify acidomonas methanolica as a pathogen associated with adenitis in a patient with cgd. | 2012 | 22752310 |
| serologic reactivity to the emerging pathogen granulibacter bethesdensis. | granulibacter bethesdensis is a recently described member of the acetobacteraceae family that has been isolated from patients with chronic granulomatous disease (cgd). its pathogenesis, environmental reservoir(s), and incidence of infection among cgd patients and the general population are unknown. | 2012 | 22782953 |
| structure and functional characterization of pyruvate decarboxylase from gluconacetobacter diazotrophicus. | bacterial pyruvate decarboxylases (pdc) are rare. their role in ethanol production and in bacterially mediated ethanologenic processes has, however, ensured a continued and growing interest. pdcs from zymomonas mobilis (zmpdc), zymobacter palmae (zppdc) and sarcina ventriculi (svpdc) have been characterized and zmpdc has been produced successfully in a range of heterologous hosts. pdcs from the acetobacteraceae and their role in metabolism have not been characterized to the same extent. examples ... | 2014 | 25369873 |
| complete genome and gene expression analyses of asaia bogorensis reveal unique responses to culture with mammalian cells as a potential opportunistic human pathogen. | asaia bogorensis, a member of acetic acid bacteria (aab), is an aerobic bacterium isolated from flowers and fruits, as well as an opportunistic pathogen that causes human peritonitis and bacteraemia. here, we determined the complete genomic sequence of the as. bogorensis type strain nbrc 16594, and conducted comparative analyses of gene expression under different conditions of co-culture with mammalian cells and standard aab culture. the genome of as. bogorensis contained 2,758 protein-coding ge ... | 2015 | 26358298 |
| prerequisites for amplicon pyrosequencing of microbial methanol utilizers in the environment. | the commercial availability of next generation sequencing (ngs) technologies facilitated the assessment of functional groups of microorganisms in the environment with high coverage, resolution, and reproducibility. soil methylotrophs were among the first microorganisms in the environment that were assessed with molecular tools, and nowadays, as well with ngs technologies. studies in the past years re-attracted notice to the pivotal role of methylotrophs in global conversions of methanol, which m ... | 2013 | 24046766 |
| characterization of p-hydroxycinnamate catabolism in a soil actinobacterium. | p-hydroxycinnamates, such as ferulate and p-coumarate, are components of plant cell walls and have a number of commercial applications. rhodococcus jostii rha1 (rha1) catabolizes ferulate via vanillate and the β-ketoadipate pathway. here, we used transcriptomics to identify genes in rha1 that are upregulated during growth on ferulate versus benzoate. the upregulated genes included three transcriptional units predicted to encode the uptake and β-oxidative deacetylation of p-hydroxycinnamates: cou ... | 2014 | 25266382 |
| methylotroph infections and chronic granulomatous disease. | chronic granulomatous disease (cgd) is a primary immunodeficiency caused by a defect in production of phagocyte-derived reactive oxygen species, which leads to recurrent infections with a characteristic group of pathogens not previously known to include methylotrophs. methylotrophs are versatile environmental bacteria that can use single-carbon organic compounds as their sole source of energy; they rarely cause disease in immunocompetent persons. we have identified 12 infections with methylotrop ... | 2016 | 26886412 |
| requirement of carbon dioxide for initial growth of facultative methylotroph, acidomonas methanolica mb58. | the facultative methylotrophic bacterium acidomonas methanolica mb58 can utilize c1 compounds via the ribulose monophosphate pathway. a large gene cluster comprising three components related to c1 metabolism was found in the genome. from upstream, the first was an mxa cluster encoding proteins for oxidation of methanol to formaldehyde; the second was the rmp cluster encoding enzymes for formaldehyde fixation; and the third was the cbb gene cluster encoding proteins for carbon dioxide (co2) fixat ... | 2015 | 25511787 |
| draft genomic dna sequence of the facultatively methylotrophic bacterium acidomonas methanolica type strain mb58. | acidomonas methanolica (former name acetobacter methanolicus) is a unique acetic acid bacterium capable to grow on methanol as a sole carbon source. here we report the draft genome sequencing of a. methanolica type strain mb58, showing that it contains 3,270 protein-coding genes, including the genes involved in oxidation of methanol such as mxafjgirsackl and hxlab, and oxidation of ethanol such as adhab and adhs. this article is protected by copyright. all rights reserved. | 2013 | 24330138 |