detection of methanotroph diversity on roots of submerged rice plants by molecular retrieval of pmoa, mmox, mxaf, and 16s rrna and ribosomal dna, including pmoa-based terminal restriction fragment length polymorphism profiling.the diversity of methanotrophic bacteria associated with roots of submerged rice plants was assessed using cultivation-independent techniques. the research focused mainly on the retrieval of pmoa, which encodes the alpha subunit of the particulate methane monooxygenase. a novel methanotroph-specific community-profiling method was established using the terminal restriction fragment length polymorphism (t-rflp) technique. the t-rflp profiles clearly revealed a more complex root-associated methanot ...200111526021
draft genomes of gammaproteobacterial methanotrophs isolated from terrestrial ecosystems.genome sequences of methylobacter luteus, methylobacter whittenburyi, methylosarcina fibrata, methylomicrobium agile, and methylovulum miyakonense were generated. the strains represent aerobic methanotrophs typically isolated from various terrestrial ecosystems.201526044417
diversity and habitat preferences of cultivated and uncultivated aerobic methanotrophic bacteria evaluated based on pmoa as molecular marker.methane-oxidizing bacteria are characterized by their capability to grow on methane as sole source of carbon and energy. cultivation-dependent and -independent methods have revealed that this functional guild of bacteria comprises a substantial diversity of organisms. in particular the use of cultivation-independent methods targeting a subunit of the particulate methane monooxygenase (pmoa) as functional marker for the detection of aerobic methanotrophs has resulted in thousands of sequences rep ...201526696968
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