| inhibition of dimethyl ether and methane oxidation in methylococcus capsulatus and methylosinus trichosporium. | metal-chelating or -binding agents inhibited the oxidation of dimethyl ether and methane, but not methanol, by cell suspensions of methylococcus capsulatus and methylosinus trichosporium. evidence suggests that the involvement of metal-containing enzymatic systems in the initial step of oxidation of dimethyl ether and methane. | 1976 | 4428 |
| purification and properties of the methane mono-oxygenase enzyme system from methylosinus trichosporium ob3b. | 1. a three-component enzyme system that catalyses the oxidation of methane to methanol has been highly purified from methylosinus trichosporium. 2. the components are (i) a soluble co-binding cytochrome c, (ii) a copper-containing protein and (iii) a small protein; the mol. wts. are 13 000, 47 000 and 9400 respectively. the cytochrome component cannot be replaced by similar cytochrome purified from pseudomonas extorquens or by horse heart cytochrome c. 3. the stoicheiometry suggests a mono-oxyge ... | 1977 | 15544 |
| [nomenclature of obligate methylotrophs]. | the nomenclature of obligate methylotrophs, i. e. bacteria using only reduced monocarbon compounds (methane, methanol, methylamines) as a carbon source, is dicussed. the chronology of naming taxons of methane oxidizing bacteria is presented and the rightfulness of their names is analyzed according to the rules of the international codex of bacterial nomenclature. such names as methylomonas and others which are employed while describing various physiological groups of bacteria are used in the nom ... | 1978 | 106220 |
| [pyruvate and phosphoenolpyruvate carboxylase in methylotrophs]. | the activity of pyruvate and phosphoenolpyruvate carboxylases was determined in cell extracts of obligate and facultative methylotrophs which metabolized monocarbon reduced compounds via different pathways. phosphoenolpyruvate carboxylase was found to be the only enzyme responsible for the high level of co2 fixation by methylotrophs with the serine pathway (methylosinus trichosporium, hyphomicrobium vulgare, pseudomonas methylica). methylotrophs with the hexulose phosphate pathway mehylobacter c ... | 1979 | 108526 |
| methyltrophic enzyme distribution in methylosinus trichosporium. | key enzymes involved in the oxidation and fixation of methane by methylosinus trichosporium were examined for localization within the bacterial cells. a differential centrifugation scheme following cell disruption was used to provide membrane and soluble fractions for the enzyme assays. all the methylotrophic enzymes examined were found to be soluble with this fractionation scheme. electron transport involving a cytochrome c2 with absorption peaks at 416, 522, and 550 nm and oxidative phosphoryl ... | 1975 | 123917 |
| properties and partial purification of the methane-oxidising enzyme system from methylosinus trichosporium. | | 1975 | 178534 |
| [refinement of the diagnosis of the genera and species of methane-using bacteria]. | the diagnoses of taxons have been corrected for methane assimilating bacteria of the family methylomonadaceae leadbetter 1974, of the genera methylomonas leadbetter 1974. methylococcus foster a. davis 1966, methylosinus whittenbury. phillips a. wilkinson 1970, methylocytis whittenbury, phillips a. wilkinson 1970, and of 16 species. the diagnosis of the species methylomonas rubra whittenbury, phillips a. wilkinson 1970 has been completed, and the new species methylomonas gracilis sp. nov. and met ... | 1978 | 418311 |
| [elimination of nitrous oxide by a combined bacterial culture]. | the ability of bacteria to eliminate nitrous oxide (n2o) from a gaseous phase containing 20% o2 was studied. representatives of the following physiological groups were found to be incapable of removing n2o: denitrifying bacteria (paracoccus denitrificans), nitrifying bacteria (nitrosospira briensis), carboxydobacteria (pseudomonas carboxydoflava), methane oxidizing bacteria (methylosinus trichosporium), and nitrogen fixing bacteria (bacillus polymyxa). five corynebacteria-like strains were isola ... | 1979 | 481272 |
| biotransformation of hydrocarbons and related compounds by whole organism suspensions of methane-grown methylosinus trichosporium ob 3b. | | 1979 | 486187 |
| [isolation and properties of new strains of obligate methanotrophs]. | new strains of obligate methanotrophic bacteria which assimilate only methane or methanol as the source of carbon and energy have been isolated. according to their morphology, ultrastructure, cultural and physiologo-biochemical characteristics, the bacteria were classed as methylobacter vinelandii, methylobacter bovis, methylobacter chroococcum and mehylosinus sporium. a new species methylocystis echinoides sp. nov. is described; it differs from other methanotrophs in certain morphological and p ... | 1977 | 600092 |
| ultrastruct of methylosinus trichosporium as revealed by freeze etching. | the methane-oxidizing bacterium methylosinus trichosporium forms extensive intracytoplasmic membranes that lie near the cell periphery and paralled to it. these membranes enclose cavities within the cytoplasm and exist as flattened, balloon-like vesicles. the internal membranes are passed along to both cells during budding. the bacteria accumulate poly-beta-hydroxybutyrate granules that lie in the center of the cells, neither within the internal membrane vesicles nor attached to them. intercellu ... | 1975 | 803485 |
| hydrogenase activity in nitrogen-fixing methane-oxidizing bacteria. | hydrogenase activity in cells of the nitrogen-fixing methane-oxidizing bacterium strain 41 of the methylosinus type increased markedly when growth was dependent upon the fixation of gaseous nitrogen. a direct relationship may exist between hydrogenase and nitrogenase in this bacterium. acetylene reduction was supported by the presence of hydrogen gas. | 1976 | 825038 |
| acetone production by methylobacteria. | an accumulation of acetone was observed during the metabolism of ethane and products of ethane oxidation by washed suspensions of methylosinus trichosporium ob3b. this strain possessed an acetoacetate decarboxylase and 3-hydroxybutyrate dehydrogenase, and a decline in poly-beta-hydroxybutyric acid occurred under the same conditions as acetone formation. a pathway of acetone production from poly-beta-hydroxybutyric acid via 3-hydroxybutyrate and acetoacetate was suggested. | 1976 | 825074 |
| [isolation of bacteriophages of methane oxidizing bacteria and study of their properties]. | five strains of bacteriophages were isolated for the first time in the ussr from the water of ponds, the paste of methane oxidizing bacteria and the cultural broth of the experimental plant. the strains are specific of the following species: methylostinus sporium, methylosinus trichosporium, and flavobacterium gasotypicum. bacteriophages lysing methylocystis impression. methylomonas agile and methylococcus capsulatus were not isolated so far. the fine structure of the phages, the shape of negati ... | 1976 | 827665 |
| [isolation of pure methanotrophic cultures and their properties]. | pure cultures of obligate methanotrophic bacteria were isolated from natural habitats and cultivated on media containing silica gel which provided more elective conditions than media with agar. according to their morphology, fine structure, cultural, physiological and biochemical properties, the bacteria were identified as methylosinus trichosporium, methylosinus trichosporium var. methanolicum, methylocystis parvus, methylocytis parvus var. fuscus, methylomonas methanica. | 1975 | 1107760 |
| screening for restriction endonucleases in methane-oxidizing bacteria. | 51 methane-oxidizing bacteria strains such as methylomonas methanica, m. rubra, methylococcus capsulatus, m. thermophilus, m. luteus, m. ucrainicus, m. whittenburyi, methylosinus trichosporium, m. sporium, methylocystis parvus isolated from various ecological niches and geographical regions of the ukraine and also the strains received from r. whittenbury and y. heyer were screened for restriction endonucleases. type ii restriction endonucleases were detected in imv b-3112 (= 12 b), imv b-3027 (= ... | 1992 | 1338116 |
| optimization of trichloroethylene oxidation by methanotrophs and the use of a colorimetric assay to detect soluble methane monooxygenase activity. | methylosinus trichosporium ob3b biosynthesizes a broad specificity soluble methane monooxygenase that rapidly oxidizes trichloroethylene (tce). the selective expression of the soluble methane monooxygenase was followed in vivo by a rapid colorimetric assay. naphthalene was oxidized by purified soluble methane monooxygenase or by cells grown in copper-deficient media to a mixture of 1-naphthol and 2-naphthol. the naphthols were detected by reaction with tetrazotized o-dianisidine to form purple d ... | 1990 | 1368139 |
| characterization of a methane-utilizing bacterium from a bacterial consortium that rapidly degrades trichloroethylene and chloroform. | a mixed culture of bacteria grown in a bioreactor with methane as a carbon and energy source rapidly oxidized trichloroethylene and chloroform. the most abundant organism was a crescent-shaped bacterium that bound the fluorescent oligonucleotide signature probes that specifically hybridize to serine pathway methylotrophs. the 5s rrna from this bacterium was found to be 93.5% homologous to the methylosinus trichosporium ob3b 5s rna sequence. a type ii methanotrophic bacterium, isolated in pure cu ... | 1992 | 1377902 |
| crystallization and preliminary x-ray analysis of the methane monooxygenase hydroxylase protein from methylococcus capsulatus (bath). | methane monooxygenase is a multicomponent enzyme system that catalyzes the conversion of methane to methanol in methanotrophic bacteria. catalysis occurs at non-heme dinuclear iron centers contained in the hydroxylase component of the system, a dimer of composition alpha 2 beta 2 gamma 2. the hydroxylase protein from methylococcus capsulatus (bath) has been crystallized from aqueous solutions containing polyethylene glycol, lithium sulfate, and ammonium acetate. the crystals are orthorhombic, sp ... | 1992 | 1404375 |
| hydrodynamic effects on microcapillary motility and chemotaxis assays of methylosinus trichosporium ob3b. | a study of the random motility and chemotaxis of methylosinus trichosporium ob3b was conducted by using palleroni-chamber microcapillary assay procedures. under the growth conditions employed, this methanotroph was observed qualitatively with a microscope to be either slightly motile or essentially nonmotile. however, the cells did not not respond in the microcapillary assays in the manner expected for nonmotile brownian particles. as a consequence, several hydrodynamic effects on these palleron ... | 1992 | 1444383 |
| applications of a colorimetric plate assay for soluble methane monooxygenase activity. | a straightforward method is described for screening methanotrophic colonies for soluble methane monooxygenase (smmo) activity on solid media. such activity results in the development of a colored complex between 1-naphthol, which is formed when smmo reacts with naphthalene, and o-dianisidine (tetrazotized). methanotrophic colonies expressing smmo turned deep purple when exposed successively to naphthalene and o-dianisidine. the method was evaluated within the contexts of two potential applicatio ... | 1992 | 1637160 |
| 16s ribosomal rna sequence analysis for determination of phylogenetic relationship among methylotrophs. | 16s ribosomal rnas (rrna) of 12 methylotrophic bacteria have been almost completely sequenced to establish their phylogenetic relationships. methylotrophs that are physiologically related are phylogenetically diverse and are scattered among the purple eubacteria (class proteobacteria). group i methylotrophs can be classified in the beta- and the gamma-subdivisions and group ii methylotrophs in the alpha-subdivision of the purple eubacteria, respectively. pink-pigmented facultative and non-pigmen ... | 1990 | 1693657 |
| fate of 2,2,2-trichloroacetaldehyde (chloral hydrate) produced during trichloroethylene oxidation by methanotrophs. | four different methanotrophs expressing soluble methane monooxygenase produced 2,2,2-trichloroacetaldehyde, or chloral hydrate, a controlled substance, during the oxidation of trichloroethylene. chloral hydrate concentrations decreased in these cultures between 1 h and 24 h of incubation. chloral hydrate was shown to be biologically transformed to trichloroethanol and trichloroacetic acid by methylosinus trichosporium ob3b. at elevated ph and temperature, chloral hydrate readily decomposed and c ... | 1991 | 1768109 |
| the methane monooxygenase gene cluster of methylosinus trichosporium: cloning and sequencing of the mmoc gene. | methane monooxygenase (mmo) is the enzyme responsible for the conversion of methane to methanol in methanotrophic bacteria. the soluble mmo enzyme complex from methylosinus trichosporium also oxidizes a wide range of aliphatic and aromatic compounds in a number of potentially useful biotransformations. in this study we have used heterologous dna probes from the type x methanotroph methylococcus capsulatus (bath) to isolate mmo genes from the type ii methanotroph m. trichosporium. we report here ... | 1991 | 1785954 |
| molecular analysis of the methane monooxygenase (mmo) gene cluster of methylosinus trichosporium ob3b. | the oxidation of methane to methanol in methanotrophic bacteria is catalysed by the enzyme methane monooxygenase (mm0). this multicomponent enzyme catalyses a range of oxidations including that of aliphatic and aromatic compounds and therefore has potential for commercial exploitation. this study details the molecular characterization of the soluble mmo (smmo) genes from the type ii methanotroph methylosinus trichosporium ob3b. the structural genes encoding the alpha, beta and gamma subunits of ... | 1991 | 1904125 |
| methanol suppression of trichloroethylene degradation by methylosinus trichosporium (ob3b) and methane-oxidizing mixed cultures. | the effect of methanol on trichloroethylene (tce) degradation by mixed and pure methylotrophic cultures was examined in batch culture experiments. methanol was found to relieve growth inhibition of methylosinus trichosporium (ob3b) at high (14 mg/l) tce concentrations. degradation of tce was determined by both radiolabeling and gas chromatography techniques. when cultures were grown on methanol over 10 to 14 d with 0.3 mg/l tce, ob3b degraded 16.89 +/- 0.82% (mean +/- sd) of the tce, and a mixed ... | 1991 | 1929390 |
| kinetics of chlorinated hydrocarbon degradation by methylosinus trichosporium ob3b and toxicity of trichloroethylene. | the kinetics of the degradation of trichloroethylene (tce) and seven other chlorinated aliphatic hydrocarbons by methylosinus trichosporium ob3b were studied. all experiments were performed with cells grown under copper stress and thus expressing soluble methane monooxygenase. compounds that were readily degraded included chloroform, trans-1,2-dichloroethylene, and tce, with vmax values of 550, 330, and 290 nmol min-1 mg of cells-1, respectively. 1,1-dichloroethylene was a very poor substrate. t ... | 1991 | 2036023 |
| biodegradation of low-molecular-weight halogenated hydrocarbons by methanotrophic bacteria. | low-molecular-weight halogenated hydrocarbons are susceptible to degradation by anaerobic and aerobic bacteria. the methanotrophic bacterium methylosinus trichosporium 0b3b degrades trichloroethylene more rapidly than other bacteria examined to date. expression of soluble methane monooxygenase (mmo) is correlated with high rates of biodegradation. an analysis of 16 s rrna sequences of 11 ribosomal rnas from type i, type ii and type x methanotrophs and methanol-utilizing bacteria have revealed fo ... | 1990 | 2094287 |
| effect of fixation-resin combinations and ruthenium red on elucidating outer envelope structure and surface morphology of two methanotrophic bacteria. | we examined the ultrastructure of the cell envelope in type i, methylomonas albus (bg8), and type ii, methylosinus trichosporium (ob3b), methane-oxidizing bacteria by using different fixatives, ruthenium red (rr) combinations and resins. we compared lr white and spurr embedments with the following fixations: glutaraldehyde/oso4, two glutaraldehyde-paraformaldehyde, and two different en bloc ruthenium red procedures, one utilizing oso4 and the other with glutaraldehyde/oso4 in sequential fixation ... | 1990 | 2105383 |
| formate dehydrogenase from methylosinus trichosporium ob3b. | | 1990 | 2126333 |
| oxidation of aromatic alcohols by purified methanol dehydrogenase from methylosinus trichosporium. | methanol dehydrogenase was found to be present in subcellular preparations of methanol-grown methylosinus trichosporium and occurred almost wholly in the soluble fraction of the cell. the enzyme, purified by deae-sephadex and sephadex g-100 chromatography, showed broad specificity toward different substrates and oxidized the aromatic alcohols benzyl, vanillyl, and veratryl alcohols in addition to a range of aliphatic primary alcohols. no enzyme activity was found toward the corresponding aldehyd ... | 1990 | 2193913 |
| haloalkene oxidation by the soluble methane monooxygenase from methylosinus trichosporium ob3b: mechanistic and environmental implications. | the soluble, three-protein component methane monooxygenase purified from methylosinus trichosporium ob3b is capable of oxidizing chlorinated, fluorinated, and brominated alkenes, including the widely distributed ground-water contaminant trichloroethylene (tce). the oxidation rates for the chloroalkenes were observed to be comparable to that for methane, the natural substrate, and up to 7000-fold higher than those reported for other well-defined biological systems. the competitive inhibitor tetra ... | 1990 | 2207083 |
| methane monooxygenase from methylosinus trichosporium ob3b. | | 1990 | 2280705 |
| formate dehydrogenase from the methane oxidizer methylosinus trichosporium ob3b. | formate dehydrogenase (nad+ dependent) was isolated from the obligate methanotroph methylosinus trichosporium ob3b. when the enzyme was isolated anaerobically, two forms of the enzyme were seen on native polyacrylamide gels, de-52 cellulose and sephacryl s-300 columns; they were approximately 315,000 and 155,000 daltons. the enzyme showed two subunits on sodium dodecyl sulfate-polyacrylamide gels. the mr of the alpha-subunit was 53,800 +/- 2,800, and that of the beta-subunit was 102,600 +/- 3,90 ... | 1990 | 2376564 |
| biodegradation of trichloroethylene by methylosinus trichosporium ob3b. | the methanotroph methylosinus trichosporium ob3b, a type ii methanotroph, degraded trichloroethylene at rates exceeding 1.2 mmol/h per g (dry weight) following the appearance of soluble methane monooxygenase in continuous and batch cultures. cells capable oxidizing trichloroethylene contained components of soluble methane monooxygenase as demonstrated by western blot (immunoblot) analysis with antibodies prepared against the purified enzyme. growth of cultures in a medium containing 0.25 microm ... | 1989 | 2515801 |
| isolation, characterization, and biological activity of ferredoxin-nad+ reductase from the methane oxidizer methylosinus trichosporium ob3b. | a ferredoxin-nad+ oxidoreductase (ec 1.18.1.3) has been isolated from extracts of the obligate methanotroph methylosinus trichosporium ob3b. this enzyme was shown to couple electron flow from formate dehydrogenase (nad+ requiring) to ferredoxin. ferredoxin-nad+ reductase was purified to homogeneity by conventional chromatography techniques and was shown to be a flavoprotein with a molecular weight of 36,000 +/- 1,000. this ferredoxin reductase was specific for nadh (km, 125 microm) and coupled e ... | 1989 | 2768195 |
| purification, crystallisation and preliminary x-ray diffraction characterisation of methanol dehydrogenase from methylosinus trichosporium ob3b. | methanol dehydrogenase was purified from the obligate methanotroph, methylosinus trichosporium ob3b, in two steps from disrupted biomass by aqueous two-phase partition and ion-exchange chromatography. copartitioning of a cytochrome c was dependent upon the ph at which aqueous partition was carried out. the native enzyme has a mr of 120,000, as determined by gel filtration chromatography, and consists of two identical subunits. the purified enzyme contained four electrophoretically distinct isoen ... | 1987 | 3030752 |
| regulation of two nickel-requiring (inducible and constitutive) hydrogenases and their coupling to nitrogenase in methylosinus trichosporium ob3b. | two uptake hydrogenases were found in the obligate methanotroph methylosinus trichosporium ob3b; one was constitutive, and a second was induced by h2. both hydrogenases could be assayed by measuring methylene blue reduction anaerobically or by coupling their activity to nitrogenase acetylene reduction activity in vivo in an o2-dependent reaction. the h2 concentration for half-maximal activity of the inducible and constitutive hydrogenases in both assays was 0.01 and 0.5 bar (1 and 50 kpa), respe ... | 1987 | 3115963 |
| degradation of trichloroethylene by toluene dioxygenase in whole-cell studies with pseudomonas putida f1. | toluene-induced cells of pseudomonas putida f1 removed trichloroethylene from growth media at a significantly greater initial rate than the methanotroph methylosinus trichosporium ob3b. with toluene-induced p. putida f1, the initial degradation rate varied linearly with trichloroethylene concentration over the range of 8 to 80 microm (1.05 to 10.5 ppm). at 80 microm (10.5 ppm) trichloroethylene and 30 degrees c, the initial rate was 1.8 nmol/min per mg of total cell protein, but the rate decreas ... | 1988 | 3415234 |
| [carbon dioxide fixation on 3-carbon acceptors in extracts of hydrogenomonas pantotropha and methylosinus trichosporium]. | | 1973 | 4205728 |
| the carbon assimilation pathways of methylococcus capsulatus, pseudomonas methanica and methylosinus trichosporium (ob3b) during growth on methane. | d-arabino-3-hexulose 6-phosphate was prepared by condensation of formaldehyde with ribulose 5-phosphate in the presence of 3-hexulose phosphate synthase from methane-grown methylococcus capsulatus. the 3-hexulose phosphate was unstable in solutions of ph greater than 3, giving a mixture of products in which, after dephosphorylation, allulose and fructose were detected. a complete conversion of d-ribulose 5-phosphate and formaldehyde into d-fructose 6-phosphate was demonstrated in the presence of ... | 1974 | 4377654 |
| cleavage of malyl-coenzyme a into acetyl-coenzyme a and glyoxylate by pseudomonas am1 and other c1-unit-utilizing bacteria. | 1. malyl-coa lyase was found in high activity in extracts of pseudomonas am1, pseudomonas ma, pseudomonas ms, hyphomicrobium x and methylosinus trichosporium. 2. the enzyme cleaves (2s)-malyl-coa into equimolar amounts of acetyl-coa and glyoxylate in the presence of mg(2+). 3. the specific activity of malyl-coa lyase was several-fold higher in pseudomonas am1 when grown on c(1) compounds than when grown on c(2), c(3) or c(4) compounds. this suggests that the enzyme plays a specially important ro ... | 1973 | 4772632 |
| [growth of methylosinus trichosporium strain z-1442 on whittenbury's medium]. | | 1973 | 4791166 |
| dna hybridization analysis of the nif region of two methylotrophs and molecular cloning of nif-specific dna. | dna isolated from two diazotrophic methylotrophs, the obligate methanotroph methylosinus sp. strain 6 and the methanol autotroph xanthobacter sp. h4-14, hybridized to dna fragments encoding nitrogen fixation (nif) genes from klebsiella pneumoniae. this interspecific nif homology was limited to dna fragments encoding the nitrogenase structural proteins (nifh, nifd, and nifk) and specific methylotroph dna sequences. the hybridization patterns obtained with the two methylotrophs were dissimilar, in ... | 1984 | 6321444 |
| molecular construction and characterization of nif mutants of the obligate methanotroph methylosinus sp. strain 6. | we describe here a method for constructing mutants in bacteria that are not amenable to mutant isolation by conventional means. a one-step marker exchange procedure was used to construct nitrogen fixation (nif) mutants of the obligate methane-utilizing bacterium methylosinus sp. strain 6, using transposon 5 (tn5)-containing nif genes cloned into pbr325. the resultant mutants appeared to contain defects in nif structural genes, and dna hybridization analysis showed that although one out of five h ... | 1984 | 6321452 |
| nitrite and nitrous oxide production by methylosinus trichosporium. | conditions for the production of nitrite and nitrous oxide by an obligate methanotroph, methylosinus trichosporium (ob 3b), were studied. the rate of nitrite production (v no2-) was correlated with the concentration of ammonia up to 20 mm in the presence of sufficient amounts of oxygen and inversely correlated with the amounts of methane in the system. the rate of nitrous oxide (n2o) production (v n2o) was correlated positively with v no2- and the amount of nitrite produced and inversely with th ... | 1985 | 3921227 |
| ammonia switch-off of nitrogenase from rhodobacter sphaeroides and methylosinus trichosporium: no evidence for fe protein modification. | in vivo switch-off of nitrogenase activity by nh4+ is a reversible process in rhodobacter sphaeroides and methylosinus trichosporium ob3b. the same pattern of switch-off in rhodospirillum rubrum is explained by adp-ribosylation of one of the fe protein subunits, however, no evidence of covalent modification could be found in the subunits from either r. sphaeroides or m. trichosporium. fe protein subunits from these organisms showed no variant behaviour on sds-page, nor were they 32p-labeled foll ... | 1988 | 3136733 |
| purification of a high specific activity methane monooxygenase hydroxylase component from a type ii methanotroph. | the purification of the hydroxylase component of a 3 component methane monooxygenase from the type ii methanotroph methylosinus trichosporium ob3b is reported. the enzyme (240 kda) has an (alpha beta gamma)2 subunit structure as observed for hydroxylases isolated from other type i and type ii methanotrophs, but it exhibits a 5 to 10 fold higher specific activity and is isolated in 2 to 10 fold higher yield. epr and mössbauer spectra of the hydroxylase show that it contains a coupled iron center ... | 1988 | 2840063 |
| evidence for a mu-oxo-bridged binuclear iron cluster in the hydroxylase component of methane monooxygenase. mössbauer and epr studies. | mössbauer and epr studies of a highly active hydroxylase component of methane monooxygenase isolated from methylosinus trichosporium ob3b are reported. the mössbauer spectra of the oxidized (as isolated) hydroxylase show iron in a diamagnetic cluster containing an even number of fe3+ sites. the parameters are consistent with an antiferromagnetically coupled binuclear cluster similar to those of hemerythrin and purple acid phosphatases. upon partial reduction of the hydroxylase, an s = 1/2 epr sp ... | 1988 | 2839495 |
| purification and properties of a heme-containing aldehyde dehydrogenase from methylosinus trichosporium. | | 1980 | 6779711 |
| degradation of chlorinated aliphatic hydrocarbons by methylosinus trichosporium ob3b expressing soluble methane monooxygenase. | degradation of trichloroethylene (tce) by the methanotrophic bacterium methylosinus trichosporium ob3b was studied by using cells grown in continuous culture. tce degradation was a strictly cometabolic process, requiring the presence of a cosubstrate, preferably formate, and oxygen. m. trichosporium ob3b cells degraded tce only when grown under copper limitation and when the soluble methane monooxygenase was derepressed. during tce degradation, nearly total dechlorination occurred, as indicated ... | 1989 | 2624462 |
| methane monooxygenase from methylosinus trichosporium ob3b. purification and properties of a three-component system with high specific activity from a type ii methanotroph. | methane monooxygenase has been purified from the type ii methanotroph methylosinus trichosporium ob3b. as observed for methane monooxygenase isolated from type i methanotrophs, three protein components are required: a 39.7-kda nadh reductase containing 1 mol each of fad and a [2fe-2s] cluster, a 15.8-kda protein factor termed component b that contains no metals or cofactors, and a 245-kda hydroxylase which appears to contain an oxo- or hydroxo-bridged binuclear iron cluster. through the use of s ... | 1989 | 2542319 |
| stereospecificity and other properties of a novel secondary-alcohol-specific alcohol dehydrogenase. | nad-dependent alcohol dehydrogenase from the methanol-grown methylcoccus sp. crl m1 (type i membrane), methylosinus trichosporium ob3b (type ii membrane), methylobacterium organophillum crl 26 (type ii membrane, facultative methylotroph). pseudomonas sp. atcc 21439, and pichia pastoris y-55 are secondary-alcohol-specific and that from p. pastoris y-7556 is not. this novel secondary-alcohol-specific alcohol dehydrogenase (secondary-alcohol dehydrogenase) has been purified from methanol-grown pseu ... | 1981 | 7030736 |
| exospore formation in methylosinus trichosporium. | formation of exospores in methylosinus trichosporium was examined by electron microscopy; serial sectioning was used to visualize the shape and location of the developing exospore in relation to the vegetative cell. the initial stage was the formation of a budlike enlargement on one end of the vegetative cell. the enlargement was surrounded by the exospore capsule, and the cell wall was continuous around both the cell and the developing exospore. a constriction occurred in the area where the bud ... | 1982 | 7054146 |
| [molecular nitrogen fixation in the waters of eutrophic and polyhuic lakes of the estonian ssr]. | a modified acetylene technique was used to assay the rate of molecular nitrogen fixation in estonian lakes containing methane in the hypolimnion. methods were elaborated to eliminate ethylene cooxidation by methane oxidizing bacteria. methane oxidation and nitrogen fixation were found in a narrow microaerobic zone in lakes with the stratification of temperature in the water mass; these biochemical processes occurred when the content of dissolved oxygen varied within the range of 0.1 to 0.8 mg o2 ... | 1980 | 7412622 |
| [fixation of molecular nitrogen and activity of the microflora in the bottoms of certain lakes in the estonian ssr and the rybinsk reservoir]. | the rate of molecular nitrogen fixation was determined in bottom grounds of three estonian lakes and the rybinsk water reservoir in the summer of 1977--1978. certain species of nitrogen fixing bacteria were found to be confined to lakes of certain trophic type. ecological niches with the mass growth of clostridium pasteurianum, azomonas agilis and clostridium butyricum were detected in the sediments of eutrophic lakes. ecological niches of az. insignis and cl. acetobutylicum occur in polyhumic l ... | 1980 | 7442573 |
| degradation of dimethyl nitrosamine by methylosinus trichosporium ob3b. | the degradation of dimethyl nitrosoamine (dmna) by a methanotroph, methylosinus trichosporium ob3b, was studied using 14c-labelled dmna. the organism was capable of assimilating dmna-carbon and converting it to co2. the rates of co2 production (vco2) from dmna and its cellular uptake (vp) were linearly correlated with dmna concentrations of 0.03-10 mm, which corresponded to approximately 3% of added dmna metabolized in 24 h. these rates were two to three orders of magnitude less than the rate of ... | 1990 | 2127906 |
| oxidation of deuterated compounds by high specific activity methane monooxygenase from methylosinus trichosporium. mechanistic implications. | hydrocarbon oxidations catalyzed by methane monooxygenase purified to high specific activity from the type ii methanotroph methylosinus trichosporium ob3b were compared to the same reactions catalyzed by methane monooxygenase from the type i methanotroph methylococcus capsulatus bath and liver microsomal cytochrome p-450. the two methane monooxygenases produced nearly identical product distributions, in accord with physical studies of the enzymes which have shown them to be very similar. the pro ... | 1991 | 1917992 |
| molecular genetics of methane oxidation. | biological methane oxidation is carried out by methanotrophs, bacteria that utilize methane as their sole carbon and energy source. the enzyme they contain that is responsible for methane oxidation is methane monooxygenase, the most well studied being the soluble methane monooxygenase enzyme complexes from methylococcus capsulatus (bath) and methylosinus trichosporium ob3b. in both organisms, the genes encoding soluble methane monooxygenase have been found to be clustered on the chromosome in th ... | 1994 | 7765830 |
| detection of soluble methane monooxygenase producing methylosinus trichosporium ob3b by polymerase chain reaction. | the soluble methane monooxygenase (smmo) enzyme complex of methanotrophs cometabolizes haloaliphatic compounds such as trichloroethylene. two 18-mer oligonucleotides as primary primers and a nested primer of the same length were selected to amplify specific dna sequences of the smmo gene cluster using polymerase chain reaction (pcr). two dna fragments of sizes 270 and 400 base pairs were obtained when purified dna from the methanotroph methylosinus trichosporium ob3b was used as template. the pr ... | 1994 | 7804907 |
| sequence analysis of the gene cluster encoding toluene-3-monooxygenase from pseudomonas pickettii pko1. | the nucleotide (nt) sequence and gene organization of the locus encoding the initial step of the toluene-3-monooxygenase (tbu) pathway from pseudomonas pickettii pko1 has been determined. this is the first reported nt sequence for a toluene monooxygenase which hydroxylates the c-3 position of toluene. six tightly assembled structural genes encoding several tbu were identified and were designated tbua1, tbuu, tbub, tbuv, tbua2 and tbuc. comparison of the deduced amino acid (aa) sequences of each ... | 1995 | 7867951 |
| complex formation between the protein components of methane monooxygenase from methylosinus trichosporium ob3b. identification of sites of component interaction. | kinetic, spectroscopic, and chemical evidence for the formation of specific catalytically essential complexes between the three protein components of the soluble form of methane monooxygenase from methylosinus trichosporium ob3b is reported. the effects of the concentrations of the reductase and component b on the hydroxylation activity of the reconstituted enzyme system has been numerically simulated based on a kinetic model which assumes formation of multiple high affinity complexes with the h ... | 1991 | 1845980 |
| trichloroethylene and chloroform degradation by a recombinant pseudomonad expressing soluble methane monooxygenase from methylosinus trichosporium ob3b. | soluble methane monooxygenase (smmo) from methylosinus trichosporium ob3b can degrade many halogenated aliphatic compounds that are found in contaminated soil and groundwater. this enzyme oxidizes the most frequently detected pollutant, trichloroethylene (tce), at least 50 times faster than other enzymes. however, slow growth of the strain, strong competition between tce and methane for smmo, and repression of the smmo locus by low concentrations of copper ions limit the use of this bacterium. t ... | 1994 | 8074526 |
| genetics of methane and methanol oxidation in gram-negative methylotrophic bacteria. | within the past few years, considerable progress has been made in the understanding of the molecular genetics of methane and methanol oxidation. in order to summarize this progress and to illustrate the important genetic methods employed, this review will focus on several well-studied organisms. these organisms include the gram-negative faculative methylotrophs methylobacterium extorquens, methylobacterium organophilum and paracoccus denitrificans. in addition, the obligate methanotrophs methylo ... | 1993 | 8092853 |
| preliminary crystallographic analysis of methane mono-oxygenase hydroxylase from methylosinus trichosporium ob3b. | the hydroxylase component of the enzyme methane mono-oxygenase from methylosinus trichosporium ob3b has been crystallized in the orthorhombic space group c222(1) with unit cell dimensions a = 264.5 a, b = 71.2 a, c = 139.4 a. the crystals grow as square, thick plates and diffract to beyond 2 a resolution. there is one half of the hydroxylase dimer in the asymmetric unit. | 1994 | 8107121 |
| formate dehydrogenase from methylosinus trichosporium ob3b. purification and spectroscopic characterization of the cofactors. | nad(+)-coupled formate dehydrogenase has been purified to near-homogeneity from the obligate methanotroph methylosinus trichosporium ob3b. the inclusion of stabilizing reagents in the purification buffers has resulted in a 3-fold increase in specific activity (98 microm/min/mg; turnover number 600 s-1) and as much as a 25-fold increase in yield over previously reported purification protocols. the enzyme, (molecular weight 400,000 +/- 20,000) is composed of four subunit types (alpha, 98,000; beta ... | 1991 | 1657982 |
| phenotypic characterization of copper-resistant mutants of methylosinus trichosporium ob3b. | cultures of methylosinus trichosporium ob3b grown in the presence of very low concentrations of copper synthesize a soluble methane monooxygenase (smmo) that efficiently catalyzes the oxidation of trichloroethylene and other organic pollutants. recently, we isolated five m. trichosporium ob3b mutants that express smmo activity when grown in the presence of elevated copper concentrations (p.a. phelps, s. k. agarwal, g. e. speitel, jr., and g. georgiou, appl. environ. microbiol. 58:3701-3708, 1992 ... | 1993 | 8215352 |
| soluble methane monooxygenase component b gene probe for identification of methanotrophs that rapidly degrade trichloroethylene. | restriction fragment length polymorphisms, western blot (immunoblot) analysis, and fluorescence-labelled signature probes were used for the characterization of methanotrophic bacteria as well as for the identification of methanotrophs which contained the soluble methane monooxygenase (mmo) gene and were able to degrade trichloroethylene (tce). the gene encoding a soluble mmo component b protein from methylosinus trichosporium ob3b was cloned. it contained a 2.2-kb ecori fragment. with this clone ... | 1992 | 1349468 |
| characterization of the methanotrophic bacterial community present in a trichloroethylene-contaminated subsurface groundwater site. | groundwater, contaminated with trichloroethylene (tce) and tetrachloroethylene (pce), was collected from 13 monitoring wells at area m on the u.s. department of energy savannah river site near aiken, s.c. filtered groundwater samples were enriched with methane, leading to the isolation of 25 methanotrophic isolates. the phospholipid fatty acid profiles of all the isolates were dominated by 18:1 omega 8c (60 to 80%), a signature lipid for group ii methanotrophs. subsequent phenotypic testing show ... | 1993 | 8368829 |
| ligation of the diiron site of the hydroxylase component of methane monooxygenase. an electron nuclear double resonance study. | electron nuclear double resonance (endor) spectroscopy is used to probe the coordination of the mixed valence (fe(ii).fe(iii)) diiron cluster of the methane monooxygenase hydroxylase component (mmoh-) isolated from methylosinus trichosporium ob3b. endor resonances are observed along the principal axis directions g1 = 1.94 and g3 = 1.76 from at least nine different protons and two different nitrogens. the nitrogens are strongly coupled and appear to be directly coordinated to the cluster irons. t ... | 1992 | 1309736 |
| activation of the hydroxylase of smmo from methylococcus capsulatus (bath) by hydrogen peroxide. | hydrogen peroxide can activate the non-heme binuclear iron-containing hydroxylase of soluble methane monooxygenase (smmo) from methylococcus capsulatus (bath) in the catalysis of oxidation of methane and other smmo substrates. the reductase, protein b, o2 and nadh are normally required for catalytic activity, but can be replaced by h2o2 serving as the source of both oxygen and electrons for the reaction. similar results have been observed in a different strain of mmo from methylosinus trichospor ... | 1993 | 8476925 |
| structural and immunological studies on the soluble formate dehydrogenase from alcaligenes eutrophus. | during growth with formate as the sole energy source the autotrophic bacterium alcaligenes eutrophus synthesizes a cytoplasmic formate dehydrogenase. the enzyme is a molybdo-iron-sulfur-flavo protein and the major nadh-producing system under these growth conditions, although it was estimated to constitute only 0.65% of the soluble cell protein. an electron microscopic analysis of the purified enzyme revealed that the particle is made up of four nonidentical submasses, corroborating previous stru ... | 1995 | 8561915 |
| large kinetic isotope effects in methane oxidation catalyzed by methane monooxygenase: evidence for c-h bond cleavage in a reaction cycle intermediate. | the reduced hydroxylase component (mmoh) of soluble methane monooxygenase (mmo) from methylosinus trichosporium ob3b reacts with o2 and ch4 to produce ch3oh and h2o in a single-turnover reaction. transient kinetic analysis of this reaction has revealed at least five and probably six intermediates during the turnover [lee, s.-k., nesheim, j. c., & lipscomb, j. d. (1993) j. biol. chem. 268, 21569-21577; liu, y., nesheim, j. c., lee, s.-k., & lipscomb, j. d. (1995) j. biol. chem. 270, 24662-24665]. ... | 1996 | 8756490 |
| [activity of methane-oxidizing bacteria in the adsorbed state]. | adsorption of pure cultures of methane oxidizing bacteria, methylosinus trichosporium 20 and methylococcus ucrainicus 21, on glass and coal was studied; the former strain was sorbed on both sorbents, the latter strain was sorbed on coal but not on glass. the rate of methane oxidation by the cells of adsorbed microorganisms was higher than in the case of free cells, and increased with a decrease in dimensions of the sorbent particles. | 1975 | 1207502 |
| trichloroethylene degradation and mineralization by pseudomonads and methylosinus trichosporium ob3b. | to examine the trichloroethylene (c2hcl3)-degrading capability of five microorganisms, the maximum rate, extent, and degree of c2hcl3 mineralization were evaluated for pseudomonas cepacia g4, pseudomonas cepacia g4 pr1, pseudomonas mendocina kr1, pseudomonas putida f1, and methylosinus trichosporium ob3b using growth conditions commonly reported in the literature for expression of oxygenases responsible for c2hcl3 degradation. by varying the c2hcl3 concentration from 5 microm to 75 microm, vmax ... | 1996 | 8920197 |
| whole-cell and membrane lipids of the methylotrophic bacterium methylosinus trichosporium. | the lipid composition of the methylotrophic bacterium methylosinus trichosporium was examined. whole-cell lipid distribution was 39.1% neutral lipids, 34.5% polar lipids, and 26.4% poly-beta-hydroxybutyric acid. membrane lipids were 83% phospholipids, with phosphatidylethanolamine and phosphatidylglycerol accounting for over 94% of the total. all the phospholipids had similar fatty acid compositions, with 18:1 accounting for about 87% of the total and most of the rest consisting of 16:1. similar ... | 1975 | 810477 |
| properties of the methane mono-oxygenase from extracts of methylosinus trichosporium ob3b and evidence for its similarity to the enzyme from methylococcus capsulatus (bath). | 1. the methane mono-oxygenase from methylosinus trichosporium ob3b was soluble. the only suitable electron donor was nad(p)h, neither sodium l-ascorbate nor electrons derived from the oxidation of methanol could substitute for nad(p)h. evidence is presented for the existence of an nad+-linked formaldehyde dehydrogenase. 2. mono-oxygenase activity was not inhibited by a range of potential inhibitors including potassium cyanide, amytal, carbon monoxide or various metal-chelating agents, although 8 ... | 1979 | 572296 |
| the soluble methane monooxygenase gene cluster of the trichloroethylene-degrading methanotroph methylocystis sp. strain m. | in methanotrophic bacteria, methane is oxidized to methanol by the enzyme methane monooxygenase (mmo). the soluble mmo enzyme complex from methylocystis sp. strain m also oxidizes a wide range of aliphatic and aromatic compounds, including trichloroethylene. in this study, heterologous dna probes from the type ii methanotroph methylosinus trichosporium ob3b were used to isolate souble mmo (smmo) genes from the type ii methanotroph methylocystis sp. strain m. smmo genes from strain m are clustere ... | 1997 | 9143121 |
| the soluble methane mono-oxygenase of methylococcus capsulatus (bath). its ability to oxygenate n-alkanes, n-alkenes, ethers, and alicyclic, aromatic and heterocyclic compounds. | 1. methane mono-oxygenase of methylococcus capsulatus (bath) catalyses the oxidation of various substituted methane derivatives including methanol. 2. it is a very non-specific oxygenase and, in some of its catalytic properties, apparently resembles the analogous enzyme from methylomonas methanica but differs from those found in methylosinus trichosporium and methylomonas albus. 3. co is oxidized to co2. 4. c1-c8 n-alkanes are hydroxylated, yielding mixtures of the corresponding 1- and 2-alcohol ... | 1977 | 411486 |
| recalcitrance of 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene (dde) to cometabolic degradation by pure cultures of aerobic and anaerobic bacteria. | pure cultures of aerobic and anaerobic bacteria capable of oxidation and reductive dehalogenation of chloroethylenes, and aerobic bacteria involved in biodegradation of polychlorinated biphenyls (pcbs) were screened for their ability to cometabolize the persistent pollutant 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene (dde). bacterial cultures expressing methane monooxygenase (methylosinus trichosporium), propane monooxygenase (mycobacterium vaccae) and biphenyl 2,3-dioxygenase enzymes (pseudomo ... | 1997 | 9294241 |
| effect of selected monoterpenes on methane oxidation, denitrification, and aerobic metabolism by bacteria in pure culture. | selected monoterpenes inhibited methane oxidation by methanotrophs (methylosinus trichosporium ob3b, methylobacter luteus), denitrification by environmental isolates, and aerobic metabolism by several heterotrophic pure cultures. inhibition occurred to various extents and was transient. complete inhibition of methane oxidation by methylosinus trichosporium ob3b with 1.1 mm (-)-alpha-pinene lasted for more than 2 days with a culture of optical density of 0.05 before activity resumed. inhibition w ... | 1998 | 9464387 |
| acidophilic methanotrophic communities from sphagnum peat bogs. | highly enriched methanotrophic communities (> 25 serial transfers) were obtained from acidic ombrotrophic peat bogs from four boreal forest sites. the enrichment strategy involved using media conditions that were associated with the highest rates of methane uptake by the original peat samples, namely, the use of diluted mineral medium of low buffering capacity, moderate incubation temperature (20 degrees c), and ph values of 3 to 6. enriched communities contained a mixture of rod-shaped bacteria ... | 1998 | 9501432 |
| [localization of energy generators in methane oxidizing bacteria]. | cytochromes a, b and c, and their quantitative distribution in the cells, were studied by means of differential spectra in obligate methane oxidizing bacteria, methylosinus trichosporium with the serine pathway of methane carbon assimilation and methylomonas agile with the ribulose phosphate pathway of methane carbon assimilation, and different types of topography of intracytoplasmic membranes. the membranes are involved in processes of coupled respiration which was confirmed by cytochemical rea ... | 1976 | 185500 |
| copper-binding compounds from methylosinus trichosporium ob3b. | two copper-binding compounds/cofactors (cbcs) were isolated from the spent media of both the wild type and a constitutive soluble methane monooxygenase (smmoc) mutant, pp319 (p. a. phelps et al., appl. environ. microbiol. 58:3701-3708, 1992), of methylosinus trichosporium ob3b. both cbcs are small polypeptides with molecular masses of 1,218 and 779 da for cbc-l1 and cbc-l2, respectively. the amino acid sequence of cbc-l1 is s?mypgs?m, and that of cbc-l2 is spmp?s. copper-free cbcs showed absorpt ... | 1998 | 9658004 |
| maldi-tof analysis of polymerase chain reaction products from methanotrophic bacteria. | polymerase chain reaction (pcr) assays were designed to amplify 56- and 99-base regions of the pmoa gene from methylosinus trichosporium ob3b and methylomicrobium albus bg8, two species of methanotrophic bacteria that are of interest for monitoring bioremediation activity. the pcr product sizes are in a mass range that is accessible to analysis by maldi-tof mass spectrometry. a rapid purification procedure using commercially available reversed-phase cartridges was applied prior to maldi-tof anal ... | 1998 | 9666732 |
| (1-14c) acetate assimilation by obligate methylotrophs, pseudomonas methanica and methylosinus trichosporium. | the oxidation of one carbon compounds (methane, methanol, formaldehyde, formate) and primary alcohols (ethanol, propanol, butanol) supported the assimilation of [1-14c]acetate by cell suspensions of type i obligate methylotroph, pseudomonas methanica, texas strain, and type ii obligate methylotroph, methylosinus trichosporium, strain pg. the amount of oxygen consumed and substrate oxidized correlated with the amount of [1-14c]acetate assimilated during oxidation of c-1 compounds and primary alco ... | 1979 | 122051 |
| a comparison of the substrate and electron-donor specificities of the methane mono-oxygenases from three strains of methane-oxidizing bacteria. | 1. methane mono-oxygenase from methylosinus trichosporium has the same broad substrate specificity as the analogous enzyme from methylococcus capsulatus (bath); the enzyme from methylomonas methanica is more specific. 2. contrary to previous reports, nad(p)h and not ascorbate is the required electron donor for the enzyme from methylosinus trichosporium. 3. it is concluded that these three bacteria contain similar methane mono-oxygenases. | 1979 | 106847 |
| methanotrophs and methanogens in masonry | methanotrophs were present in 48 of 225 stone samples which were removed from 19 historical buildings in germany and italy. the average cell number of methanotrophs was 20 cfu per g of stone, and their activities ranged between 11 and 42 pmol of ch4 g of stone-1 day-1. twelve strains of methane-oxidizing bacteria were isolated. they belonged to the type ii methanotrophs of the genera methylocystis, methylosinus, and methylobacterium. in masonry, growth substrates like methane or methanol are ava ... | 1998 | 9797318 |
| properties of the membranes containing the particulate methane monooxygenase from methylosinus trichosporium ob3b. | the particulate methane monooxygenase (pmmo) from methylosinus trichosporium ob3b was partially purified and characterized by measuring the effects of reducing agents and additives, and the stability of pmmo was studied. duroquinol was a suitable reducing agent, and pmmo was stabilized by bovine serum albumin (bsa). among the additives, the copper (ii) ion stimulated pmmo at low concentration and inhibited at high concentration. the optimum conditions for pmmo activity were as follows: 45 degree ... | 1998 | 9850566 |
| cytochrome p460 genes from the methanotroph methylococcus capsulatus bath. | p460 cytochromes catalyze the oxidation of hydroxylamine to nitrite. they have been isolated from the ammonia-oxidizing bacterium nitrosomonas europaea (r. h. erickson and a. b. hooper, biochim. biophys. acta 275:231-244, 1972) and the methane-oxidizing bacterium methylococcus capsulatus bath (j. a. zahn et al., j. bacteriol. 176:5879-5887, 1994). a degenerate oligonucleotide probe was synthesized based on the n-terminal amino acid sequence of cytochrome p460 and used to identify a dna fragment ... | 1998 | 9851984 |
| [formation of the taxa of methane-oxidizing bacteria by numerical analysis methods]. | obligate methane oxidizing bacteria were classified within groups using numerical analysis and computer techniques. employment of properties coinciding for all the species of the bacterial group under study was found to result in an insignificant (5--10%) change in the degree of similarity rather than in the redistribution of interrelationship; it had no effect on the formation of phenons. the smirnov taxonomical analysis according to which the weight of a property is inversely proportional to i ... | 1978 | 101743 |
| phospholipid composition of methane-utilizing bacteria. | the phospholipids of methylococcus capsulatus, methylosinus trichosporium, la paz, and obt were examined in relation to their qualitative and quantitative composition. m. capsulatus exhibited a phospholipid composition consisting of phosphatidylethanolamine, phosphatidylglycerol, cardiolipin, and phosphatidyl-choline. the esterified fatty acids were predominantly c16:0 and c16:1. m. trichosporium, la paz, and obt exhibited an essentially identical phospholipid composition consisting of phosphati ... | 1978 | 96101 |
| alcohol dehydrogenase from methylobacterium organophilum. | the alcohol dehydrogenase from methylobacterium organophilum, a facultative methane-oxidizing bacterium, has been purified to homogeneity as indicated by sodium dodecyl sulfate-gel electrophoresis. it has several properties in common with the alcohol dehydrogenases from other methylotrophic bacteria. the active enzyme is a dimeric protein, both subunits having molecular weights of about 62,000. the enzyme exhibits broad substrate specificity for primary alcohols and catalyzes the two-step oxidat ... | 1978 | 80974 |
| extraction and some properties of soluble methane monooxygenase of methylosinus trichosporium imv 3011. | | 1998 | 9928147 |
| demonstration of efficient trichloroethylene biodegradation in a hollow-fiber membrane bioreactor. | rapid cometabolism of trichloroethylene (tce) by pure cultures of methylosinus trichosporium ob3b pp358 was demonstrated in a two-stage hollow-fiber membrane bioreactor over the course of 3 weeks. pp358 was grown in a continuous-flow chemostat and circulated through the shell of a hollow-fiber membrane module (hfmm), while tce contaminated water (160 to 1450 micrograms/l) was pumped through the fiber lumen (fiber interior). in parallel-flow hfmm biological experiments, 82% to 89% of the influent ... | 1999 | 9951524 |
| high-affinity methane oxidation by a soil enrichment culture containing a type ii methanotroph. | methanotrophic bacteria in an organic soil were enriched on gaseous mixing ratios of <275 parts per million of volume (ppmv) of methane (ch4). after 4 years of growth and periodic dilution (>10(20) times the initial soil inoculum), a mixed culture was obtained which displayed an apparent half-saturation constant [km(app)] for ch4 of 56 to 186 nm (40 to 132 ppmv). this value was the same as that measured in the soil itself and about 1 order of magnitude lower than reported values for pure culture ... | 1999 | 10049856 |
| homologous expression of soluble methane monooxygenase genes in methylosinus trichosporium ob3b. | an homologous expression system has been developed for soluble methane monooxygenase (smmo) genes from methylosinus trichosporium ob3b. smmo-minus mutants were previously obtained after marker-exchange mutagenesis, by the insertion of a kanamycin-resistance cassette into the mmox gene of the smmo operon. complementation of the smmo-minus genotype was achieved by conjugation with broad-host-range plasmids containing the native promoter and smmo operon from ms. trichosporium ob3b (pvk100sc and phm ... | 1999 | 10075428 |
| trichloroethene degradation in a two-step system by methylosinus trichosporium ob3b. optimization of system performance: use of formate and methane. | the breakdown of dissolved tce in a two-step bioremediation system is described. in the first reactor, the organism methylosinus trichosporium ob3b is grown; in the second reactor, consisting of three 17-l column reactors in series, the cells degrade tce. a special design allowed both for the addition of air (ug,s = 0.01-0. 04 mm s-1) in the conversion reactor to prevent oxygen limitation while minimizing stripping of tce, and for the use of methane as exogenous electron donor. in two-step syste ... | 1999 | 10099581 |
| microbial oxidation of gaseous hydrocarbons: epoxidation of c2 to c4 n-alkenes by methylotrophic bacteria. | over 20 new cultures of methane-utilizing microbes, including obligate (types i and iii) and facultative methylotrophic bacteria were isolated. in addition to their ability to oxidize methane to methanol, resting cell-suspensions of three distinct types of methane-grown bacteria (methylosinus trichosporium ob3b [type ii, obligate]; methylococcus capsulatus crl m1 nrrl b-11219 [type i, obligate]; and methylobacterium organophilum crl-26 nrrl b-11222 [facultative]) oxidize c2 to c4 n-alkenes to th ... | 1979 | 39502 |
| microbial oxidation of methane and methanol: crystallization and properties of methanol dehydrogenase from methylosinus sporium. | obligate methylotrophs are divisible into two types on the basis of ultrastructural biochemical characteristics. both groups possess a soluble phenazine methosulfate (pms)-dependent methanol dehydrogenase. in addition, particulate pms-dependent methanol dehydrogenase and pms-independent methanol oxidase have been found in the type i membrane group. a procedure was developed for the crystallization of methanol dehydrogenase from the soluble fraction of the type ii obligate methylotroph methylosin ... | 1976 | 10274 |