| effect of cadmium on fungi and on interactions between fungi and bacteria in soil: influence of clay minerals and ph. | fungi (rhizopus stolonifer, trichoderma viride, fusarium oxysporum f. sp. conglutinans, cunninghamella echinulata, and several species of aspergillus and penicillium) tolerated higher concentrations of cadmium (cd) when grown in soil than when grown on laboratory media, indicating that soil mitigated the toxic effects of cd. in soil amended with clay minerals, montmorillonite provided partial or total protection against fungistatic effects of cd, whereas additions of kaolinite provided little or ... | 1977 | 18085 |
| agrobactin, a siderophore from agrobacterium tumefaciens. | a siderophore (microbial iron transport compound) was isolated from low iron cultures of agrobacterium tumefaciens b6. the substance was characterized as a threonyl peptide of spermidine acylated with 3 residues of 2,3-dihydroxybenzoic acid, the carbonyl group of 1 residue of the latter participating in an oxazoline ring with the beta-hydroxyl of the threonine moiety. the compound, n-[3-(2,3-dihydroxybenzamido)propyl]-n-[4-(2,3-dihydroxybenzamido)butyl]-2-(2,3-dihydroxyphenyl)-trans-5-methyl-oxa ... | 1979 | 33987 |
| interactions and dna transfer between agrobacterium tumefaciens, the ti-plasmid and the plant host. | agrobacterium tumefaciens is a gram-negative bacterium with the unique capacity to induce neoplasmic transformations in dicotyledonous plants. recently, both the mechanism and the biological significance of this transformation have been elucidated. agrobacterium tumefaciens strains contain a large extrachromosomal dna plasmid (the ti-plasmid). this ti-plasmid is responsible for the oncogenic properties of agrobacterium strains. a particular segment of the ti-plasmid, containing information deter ... | 1979 | 36626 |
| fine structure and distribution of extracellular polymer surrounding selected aerobic bacteria. | the structure and distribution of extracellular polymer surrounding bacillus circulans, diplococcus (streptococcus) pneumoniae, streptococcus salivarius, staphylococcus aureus, klebsiella pneumoniae, pseudomonas aeruginosa, herella vaginacola (acinetobacter calcoaceticus), and agrobacterium tumefaciens were studied by electron microscopy. a modified ruthenium red staining procedure was used to examine the fine structure of capsule and slime. freeze-etching and critical-point drying were used to ... | 1975 | 46774 |
| proceedings: studies on large dna plasmids of agrobacterium tumefaciens. | | 1975 | 54090 |
| particulate cytochrome c in agrobacterium tumefaciens. | in agrobacterium tumefaciens the main part of c-type cytochromes is tightly bound to the bacterial cell envelope structures. several techniques were attempted to solubilize these cytochromes. the highest yield of cytochromes released is obtained by treatment of particle suspensions with 5% triton x-100. further purification confirms that the proteins are not really solubilized, but still aggregated in small heterogeneous complexes. chromatography on a cm-cellulose column demonstrates that at lea ... | 1975 | 58606 |
| purification of the agrobacterium radiobacter 84 agrocin. | | 1975 | 58630 |
| [a rna extract from oncogenic and non oncogenic strains of agrobacterium tumefaciens is an indispensable element for the induction of tumors in datura stramomium]. | an rna bound to the reverse transcriptase of agrobacterium tumefaciens has been isolated and shown to be oncogenic for stem tissues of datura stramonium grown under axenic conditions. the tumorous nature of the cellular change induced by the infectious rna was demonstrated by serial grafts of tumors on datura stems and by cultivation of tumorous tissue in vitro on a medium without supplemental auxins and cytokinins. active cellular proliferation within tissues of datura stems was a prerequisite ... | 1976 | 58705 |
| proceedings: l-sorbose metabolism in agrobacterium: a biochemical explanation for an intrageneric phenotypic difference. | | 1976 | 60964 |
| sequence of the haem-binding region of cytochrome c-556 from agrobacterium tumefaciens, strain b2a [proceedings]. | | 1979 | 93467 |
| studies on regulatory functions of malic enzymes. v. comparative studies of malic enzymes in bacteria. | screening of four malic enzymes--nad-linked enzyme [ec 1.1.1.38], nad, nadp-linked enzyme [ec 1.1.1.39], nadp-linked enzyme [ec 1.1.1.40], and d-malic enzyme--was carried out with cell-free extracts of the following 16 strains of bacteria by the aid of sepharose 6b column chromatography: 9 strains of enteric bacteria, 3 strains of pseudomonas, alcaligenes faecalis, agrobacterium tumefaciens, rhodospirillum rubrum, and clostridium tetanomorphum. all the strains tested contained at least one malic ... | 1978 | 96110 |
| isolation of large bacterial plasmids and characterization of the p2 incompatibility group plasmids pmg1 and pmg5. | large plasmids from agrobacterium tumefaciens, salmonella typhimurium, escherichia coli, pseudomonas putida, and pseudomonas aeruginosa were routinely and consistently isolated using a procedure which does not require ultracentrifugation but includes steps designed to separate large-plasmid dna from the bacterial folded chromosome. it also selectively removes fragments of broken chromosome. a variety of large plasmids was readily visualized with agarose gel electorphoresis, including five betwee ... | 1978 | 97269 |
| [stimulation of induction or inhibition of crown-gall tumor development by rna-fragments u2. interference by auxin]. | rna-fragments u2 obtained by mild degradation with rnase u2 of ribosomal rna containing a and g nucleotides in excess are capable of exhibiting either a stimulatory effect on the induction of crown-gall tumors or an inhibitory action on their subsequent development. these different effects are dependent on the moment at which rna-fragments were introduced into wounded pea seedlings infected by agrobacterium tumefaciens b6. the results obtained in vitro and in vivo suggest that an interaction bet ... | 1979 | 111819 |
| microorganisms in the rhizosphere of wheat colonized by the fungus gaeumannomyces graminis var. tritici. | the population of microorganisms in wheat rhizosphere changed in the presence of the fungus gaeumannomyces graminis var. tritici causing the take-all of wheat. in the majority of cases when the soil was artificially contaminated by the fungus, both the number of bacteria in the rhizosphere and the bacteria/fungi ratio temporarily increased. at the beginning bacteria growing in the presence of nh4+ predominated, later bacteria utilizing organic n-substances prevailed. pseudomonas fluorescens and ... | 1979 | 112016 |
| characterization and comparison of chloramphenicol acetyltransferase variants. | 1. variants of chloramphenicol acetyltransferase from a variety of bacterial species have been isolated and purified to homogeneity. they constitute a heterogeneous group of proteins as judged by analytical affinity and hydrophobic ('detergent') chromatography, native and sodium dodecyl sulfate electrophoresis, sensitivity to sulfhydryl specific reagents, steady state kinetic analysis, and reaction with antisera. 2. the most striking observation is that three variants of chloramphenicol acetyltr ... | 1979 | 116849 |
| [the influence of protoplast media on the tumor induction on kalanchoe leaves]. | experiments concerning tumor transformation of plant cells or protoplasts need complete knowledge of the effects of media components. here inhibiting effects of cellulase onozuka, glucose, sucrose and mannitol (up to 0.3m), of tissue culture media, and of the antibiotic aureomycin on the tumor initiation process in kalanchoe leaves are reported. also the influence of these substances on the growth of the tumor inducing agrobacterium tumefaciens is studied. the results lead to the design of condi ... | 1975 | 123395 |
| carbohydrate catabolism of selected strains in the genus agrobacterium. | radiorespirometric and enzyme analyses were used to reveal the glucose-catabolizing mechanisms functioning in single strains of seven presumed agrobacterium species. the entner-doudoroff and pentose cycle pathways functioned in a. radiobacter, a. tumefaciens, a. rubi, and a. rhizogenes. whereas both catabolic pathways were utilized to an almost equal degree in the a. radiobacter and a. tumefaciens strains, use of the entner-doudoroff pathway predominated in the a. rubi and a. rhizogenes strains. ... | 1975 | 128316 |
| [nucleic acids during tumorous transformation in plants]. | crown-gall, one of the "plant cancer" is induced in the presence of a soil bacterium agrobacterium tumefaciens which elaborates a tumor inducing principle (t.i.p.), the nature of which is unknown. several informations suggest that some dna sequences of bacterial origin are included in tumorous cells of tissue cultures. a ribonuclease inhibits induction. an rna extracted from agrobacterium induces some hyperplasia transplantable by graft. | 1975 | 130189 |
| [tumorous nature of hyperplasia obtained experimentally]. | the determination of the tumorous nature of an overgrowth provides one of the most essential information for all research concerning animal and plant cancerisation. the experimental procedure which will be described here answers to this imperative: it allows to prove the tumorous characteristics of proliferations obtained by inoculation of agrobacterium tumefaciens rna fractions to datura stems. | 1975 | 130191 |
| effect of three nematicides on the growth of some phytopathogenic bacteria and fungi. | the effect of three nematicides, aldicarb, fensulfothion, and phenamiphos at four concentrations (1, 5, 25, and 125 ppm) was tested on the growth of five bacteria, agrobacterium tumefaciens, corynebacterium fascians, erwinia carotovora, pseudomonas solanacearum, and streptomyces scabies and four fungi, fusarium oxysporum f. sp. vasinfectum, fusarium solani, rhizoctonia solani, and sclerotium bataticola. of the bacteria, p. solanacearum was most affected by the chemicals at all concentrations, wh ... | 1977 | 143857 |
| in vivo transfer of the ti-plasmid of agrobacterium tumefaciens to escherichia coli. | the ti-plasmids are naturally self-transmissible from their normal host agrobacterium to e. coli. they are however unable to stably establish themselves as a replicon in e. coli. it is nevertheless possible to study the ti-plasmids in e. coli with the help of ti::rp4 cointegrate plasmids that transfer and maintain themselves very efficiently in e. coli. an e. coli harbouring such a ti::rp4 plasmid is unable to catabolize octopine and unable to induce crown-gall tumours on plants. | 1978 | 150536 |
| differential toxicities of mercury to bacteria and bacteriophages in sea and in lake water. | mixtures of anionic hgcl3-/hgcl4(2)-complexes were less toxic to terrestrial bacteria (erwinia herbicola, agrobacterium tumefaciens), to marine bacteria (acinetobacter sp., aeromonas sp.), and to bacteriophages (phi 11 m 15 of staphylococcus aureus and p1 of escherichia coli) than were equivalent concentrations of hg as cationic hg2+. the toxicity of 1 ppm hg to a. tumefaciens. aeromonas sp., and phi 11 m 15 was less in seawater than in lake water. inasmuch as the hg-cl species are formed in env ... | 1979 | 161510 |
| transfer of the tumor inducing factor in agrobacterium tumefaciens. | | 1975 | 164858 |
| hexopyranoside: cytochrome c oxidoreductase from agrobacterium. | | 1975 | 165382 |
| purification and properties of cytochrome c-556 from agrobacterium tumefaciens b2a. | cytochrome c-556 from agrobacterium mefaciens b2a was isolated in a pure, homoneous state. the best purification procedure volved ammonium sulphate fractionation, delting on sephadex g-25, column chromatographic fractionation on deae- and cm-cellulose, and gel filtration on sephadex g-75 superfine. substitution of the cm-cellulose step by isoelectric focusing was successful. the purity of the final preparation is warranted by the purity index value, the electrophoretic patterns in the absence an ... | 1975 | 170180 |
| soluble proteins and hydrolases during crown-gall induction in the tomato, lycopersicon esculentum. | soluble proteins isolated from tissues of the tomato lycopersicon esculentum, after inoculation with agrobacterium tumefaciens to induce tumours, have been examined by gel electrophoresis and cytochemically. changes that occur include the suppression of host enzymes, the appearance of bacterial enzymes in the host tissues and the appearance of new enzyme bands in the affected cells. these changes are detectable within 6 hr of infection and prior to evident morphological changes, and may be expl ... | 1976 | 178634 |
| a ferrodoxin from agrobacterium tumefaciens. | | 1975 | 179857 |
| dna modifying enzymes of agrobacterium tumefaciens: effect of dna topoisomerase, restriction endonuclease, and unique dna endonuclease on plasmid and plant dna. | extracts from agrobacterium tumefaciens strain id135 contain three enzymes that have been characterized and partially purified. the first enzyme, a dna topoisomerase, appeared to relax only negatively twisted dna. the second enzyme, atu i, a type ii restriction endonuclease, generated the identical dna digestion pattern as ecorii when several dnas were used. the third enzyme, endonuclease a, showed a preference for superhelical dnas as substrates. when plasmid pck135dna, obtained from the virule ... | 1978 | 212732 |
| polymyxin resistance in agrobacterium tumefaciens and its effect on crown gall tumor induction. | polymyxin-resistant (pblr) mutants of agrobacterium tumefaciens a6, b6, and b6m were isolated from polymyxin-sensitive (pbls) parent strains in a defined medium containing 600 microgram of polymyxin b sulfate per millilitre. the weight and number of tumors induced by pblr mutants on a variety of host plants such as carrot, potato, and pinto bean were 45--75% less than those induced by pbls wild types. the crude cell envelopes (cce) prepared from both pbls and pblr bacteria were inhibitory for tu ... | 1979 | 219950 |
| a c-terminal haem-bound cytochrome c-556 from agrobacterium tumefaciens, strain b-2a. amino acid composition and n-terminal sequence analysis. | | 1979 | 221262 |
| involvement of a plasmid in the hairy root disease of plants caused by agrobacterium rhizogenes. | | 1979 | 231271 |
| rapid mapping of transposon insertion and deletion mutations in the large ti-plasmids of agrobacterium tumefaciens. | a procedure is presented, that has allowed the rapid assignment of transposon tn1 and tn7 insertion sites in the large (130 md) nopaline ti-plasmid ptic58, to specific restriction enzyme fragments. total bacterial dna is isolated from agrobacterium tumefaciens strain c58 mutants that carry a transposon in their ti-plasmid, and digested with an appropriate restriction endonuclease. the fragments are separated on an agarose gel, denatured and transferred to nitrocellulose filters. these are hybrid ... | 1979 | 231764 |
| agrobacterium ti plasmids as a tool for genetic engineering in plants. | | 1979 | 233066 |
| small-scale techniques for the analysis of recombinant plasmids. | using the cloning of part of the t-dna of ptic58 from agrobacterium tumefaciens as an example, techniques are described which enable recombinant plasmids to be mapped and used as hybridization probes. in all cases the starting material is a colony of cells grown on an agar plate which is then subjected to lysis by lysozyme and triton x-100 in volumes of the order of 300 microliters thus eliminating the need for handling and centrifuging liquid cultures under restrictive containment conditions. | 1979 | 233238 |
| transposition of plasmid dna segments specifying hydrocarbon degradation and their expression in various microorganisms. | the conjugative tol plasmid (75 mdal), specifying biodegradation of xylenes, toluene, and trimethylbenzene derivatives, undergoes dissociation in pseudomonas aeruginosa pao to a nonconjugative tol(*) plasmid (28 mdal) and a transfer plasmid termed toldelta (48 mdal). the tol(*) plasmid is rendered transmissible through introduction of a number of conjugative plasmids such as factor k, cam, and toldelta but not by the fp2 derivative pr0271. transfer of tol(*) via factor k or toldelta is mediated ... | 1978 | 277912 |
| proteins conferred by the virulence-specifying plasmid of agrobacterium tumefaciens c-58. | membrane-associated and periplasmic proteins of agrobacterium tumefaciens c-58 were compared with those from avirulent (nontumrigenic) derivative strains by slab and two-dimensional gel electrophoresis. two proteins (per-i and per-2), with a molecular weight of 37,500 and 37,300, respectively, were detected in the supernatant fraction of cells of strain c-58 treated with edta and lysozyme in which a 117-megadalton plasmid confers virulence on the organism. the same proteins are missing in an av ... | 1978 | 278990 |
| molecular cloning as a tool to the analysis of the ti plasmids of agrobacterium tumefaciens [proceedings]. | | 1978 | 289306 |
| arginine catabolism: a new function of both octopine and nopaline ti-plasmids of agrobacterium. | | 1979 | 289898 |
| isolation of a recombination deficient agrobacterium tumefaciens mutant. | the isolation of a recombination deficient (rec-) strain of agrobacterium tumefaciens is described. strain lba 4011 was mutagenized with nitrosoguanidine and after segregation 18,000 colonies were replica plated and uv irradiated. twentytwo uv sensitive strains were isolated and tested for methylmethanesulphonate (mms) sensitivity. six of these strains were more mms-sensitive than lba 4011. a ti plasmid that was genetically marked with tn 1 (cbr) was introduced in these strains and the rescue of ... | 1979 | 290851 |
| intergeneric mobilization of rhizobium nif genes to agrobacterium and klebsiella. | | 1979 | 290853 |
| replica-plating virulence assay for agrobacterium tumefaciens. | replica plating onto wounded tobacco leaves provides a convenient method for screening for avirulent mutants of agrobacterium tumefaciens. | 1977 | 330393 |
| the ti-plasmid of agrobacterium tumefaciens, a natural vector for the introduction of nif genes in plants? | | 1977 | 336023 |
| transfer of nitrogen fixation genes from a bacterium with the characteristics of both rhizobium and agrobacterium. | strain t1k, reported to be rhizobium trifolii strain t1 carrying the drug resistance plasmid ru-1drd, was able to transfer a cluster of nif+ genes to escherichia coli k-12. additional genetic material, resembling the gal-chla region of e. coli, was also transferred from strain t1k. the segregation pattern of these transferred genes suggested that they were on a plasmid. although strain tik was able to nodulate red and white clover, it also formed very slow-growing galls on tomato stems and share ... | 1978 | 342496 |
| transfection and transformation of agrobacterium tumefaciens. | the freeze thaw transfection procedure of dityatkin et al. (1972) was adapted for the transfection and transformation of a. tumefaciens. transfection of the strains b6s3 and b6-6 with dna of the temperate phage ps8cc186 yielded a maximum frequency of 2 10(-7) transfectants per total recipient population. in transformation of the strain gv3100 with the p type plasmid rp4 a maximum frequency of 3.5 10(-7) transformants per total recipient population was obtained. agrobacterium ti-plasmids were int ... | 1978 | 355847 |
| [the classification of agrobacterium and rhizobium phages (author's transl)]. | all phages studied are tailed, belong to six morphological groups, and are classified by morphological and serological properties and by physico-chemical parameters of the virion and its nucleic acid. four species of agrobacterium and 12 species of rhizobium phages are described, and their relationship with enterobacteria phages discussed. they include 68 viruses, whereas 60 poorly known phages have not been classified. | 1978 | 370746 |
| transfer of rp4::mu plasmids to agrobacterium tumefaciens. | | 1978 | 372975 |
| crown-gall and agrobacterium tumefaciens: survey of a plant-cell-transformation system of interest to medicine and agriculture. | | 1979 | 377583 |
| transcription of ti plasmid-derived sequences in three octopine-type crown gall tumor lines. | total rna isolated from three octopine-type crown gall lines contains sequences homologous to specific regions of the tumor-inducing (ti) plasmid of agrobacterium tumefaciens strain 15955. a comparison of transcripts in these three tumor lines suggests that tumor cells transcribe various sequences within a sector of plasmid dna of 13 x 10(6) daltons and that transcription may not be uniform across the plasmid derived sequences (t-dna). transcription of t-dna by octopine-type tumors occurs at fou ... | 1979 | 379864 |
| primary structure of a chloramphenicol acetyltransferase specified by r plasmids. | naturally occurring isolates of chloramphenicol-resistant bacteria commonly synthesise chloramphenicol acetyltransferase (ec 2.3.28; cat) in amounts which are sufficient to account for the resistance phenotype and often harbour plasmids which carry the structural gene for cat. the findings of cat in such diverse prokaryotes as proteus mirabilis, agrobacterium tumefaciens, streptomyces sp., and a soil flavobacterium has led to speculation concerning the origin and evolution of the more commonly o ... | 1979 | 390404 |
| tumor induction by agrobacterium tumefaciens prevented in vigna sinensis seedlings systemically infected by ribonucleic acid viruses. | cowpea (vigna sinensis) seedlings failed to develop tumors after being inoculated with crown gall bacteria (agrobacterium tumefaciens) if, at times earlier than 1 day later, they were inoculated on the primary leaves with a cowpea mosaic virus that systemically infects them. inoculation with buffer or with a virus that is restricted to a localized infection, or to which the cowpea is immune, did not interfere with the subsequent development of tumors. the virus infection did not appear to affect ... | 1979 | 422243 |
| crown gall teratoma formation is plasmid and plant controlled. | experiments using different species of the plant nicotiana and strains of the bacterium agrobacterium tumefaciens showed that teratoma formation from crown galls was dependent on the combination of bacterial ti plasmid and host plant used. | 1979 | 422509 |
| isolation of a non-tumor-inducing mutant of the ti plasmid of agrobacterium tumefaciens strain b6. | a nonpathogenic mutant of agrobacterium tumefaciens strain b6 was isolated and its properties compared with the parental strain in an effort to localize the mutation. both b6 and its mutant (b6-95) had similar colony color and morphology, were ketolactose positive, utilized octopine, and contained plasmid dna. kinetic analysis of dna reannealing showed that total dna homology and plasmid dna homology between b6 and b6-95 was at least 90%. the length of both plasmids was found to be 58 micrometer ... | 1979 | 455147 |
| genetic mapping of the bacteriophage ps8 of agrobacterium tumefaciens. | genetic mapping of the chromosome of the bacteriophage ps8 of agrobacterium tumefaciens using temperature sensitive (ts) mutants of the bacteriophage is described. | 1979 | 456950 |
| r-plasmid-mediated chromosomal gene transfer in agrobacterium tumefaciens. | although several techniques are available for transferring the ti plasmids from one strain of agrobacterium tumefaciens to another, there are no reproducible methods for analysis of chromosomal markers in this phytopathogen. the r plasmid, r68.45, is known to show chromosomal mobilizing ability in several bacterial genera including the closely related rhizobia. r68.45 was transferred into the prototrophic a. tumefaciens strain 15955. ten kanamycin-resistant transconjugant clones were tested for ... | 1979 | 457601 |
| negative control of octopine degradation and transfer genes of octopine ti plasmids in agrobacterium tumefaciens. | the regulatory system that controls the expression of the ti plasmid-borne octopine degradation (uad) and transfer (tra) genes in agrobacterium tumefaciens was studied. a deletion mutant derived from the cointegrate plasmid r702::ti-b6s3 was isolated, which was compatible with a wild-type ti plasmid and which had retained the uad genes. by means of this mutant plasmid pal116, it was possible to make cells diploid for the uad genes. pal116 was introduced into rec- strains that contained different ... | 1979 | 457610 |
| host range conferred by the virulence-specifying plasmid of agrobacterium tumefaciens. | the host range of agrobacterium tumefaciens 1d1109, known to induce crown gall only on grapevine (vitis spp.), was extended to include many plant species by transferring a tumor-inducing plasmid (pti) from strain 1d1, a broad-host-range pathogen. the pti plasmid was mobilized by the conjugative plasmid prk2, which was inserted into 1d1 by mating with escherichia coli j53(prk2). the resulting transconjugants were screened for their ability to induce crown gall tumors on hosts other than grapevine ... | 1979 | 457613 |
| effect of bacterial polysaccharides on the growth of gaeumannomyces graminis var. tritici and wheat roots. | agrobacterium sp. and related species which in the soil and in the rhizosphere of wheat accompany the fungus gaemannomyces graminis var. tritici and cause take-all of the wheat roots produced polysaccharides in pure cultures (glucans, mannoglucans and galactomannoglucans). these polysaccharides were utilized better by the mycelium of g. graminis than glucose and polysaccharides of plant origin that occurred on the surface of wheat roots (the so-called mucigel). at lower concentrations these bact ... | 1979 | 468081 |
| choriogonadotropin-like antigen in an anaerobic bacterium, eubacterium lentum, isolated from a rectal tumor. | using the indirect fluorescein-labeled and indirect peroxidase-antiperoxidase-labeled immunohistochemical techniques, and utilizing both antiserum specific for the beta-subunit of choriogonadotropin and antiserum for the total hormone, we have demonstrated the presence of a choriogonadotropin-like immunoreactive material in a strain of eubacterium lenthum that was originally isolated from a rectal tumor. in contrast, both immunohistochemical reactions were negative when applied to a strain of co ... | 1979 | 468380 |
| purification and characterization of the crown gall specific enzyme nopaline synthase. | nopaline synthase of sunflower (helianthus annuus l.) crown gall tissue induced by agrobacterium tumefaciens strain c58 or t37 (nopaline utilizers) was purified to homogeneity as judged by analytical disc gel electrophoresis. the native enzyme elutes from a column of ultrogen aca 34 as a single peak with an estimated molecular weight of 158,000. the dissociated enzyme migrates on nadodso4-polyacrylamide gels as a single band with a molecular weight of 40,000. thus, the native enzyme appears to b ... | 1979 | 476084 |
| purification and properties of d-glucosaminate dehydratase from agrobacterium radiobacter. | | 1979 | 477973 |
| a new site-specific endonuclease showing phenotypical crypticity in a tumorigenic strain of agrobacterium tumefaciens. | atubvi, an endonuclease showing new site-specificity, has been isolated from the tumorigenic strain iibv7 of agrobacterium tumefaciens, and is undetectable in the non-tumorigenic sister strain iibnv6. atubvi degrades iibv7 dna in vitro and should, therefore, be regarded as being phenotypically cryptic in the bacterial cell; it also shows anomalous behavior under cerain incubation conditions. these properties point to a possible role for this enzyme in the insertion of exogenous ti-plasmid dna in ... | 1979 | 478298 |
| indoleacetic acid production: a plasmid function of agrobacterium tumefaciens c58. | | 1979 | 496970 |
| purification and characterization of agrocin 84. | a procedure for the rapid purification of milligram quantities of agrocin 84, a bacteriocin-like compound produced by agrobacterium radiobacter strain k-84, has been developed. this procedure, which employs charcoal adsorption, ion-exchange, sieving chromatography, and continuous-flow electrophoresis, can yield agrocin 84 which is 65% pure on a dry weight basis. the purest preparations were strongly ultraviolet absorbing, with a maximum at 264 nm (epsilon 7.0/264 = 22,675 cm2 - m-1) and a minimu ... | 1979 | 507786 |
| comparative studies of polysaccharides elaborated by rhizobium, alceligenes, and agrobacterium. | | 1979 | 519656 |
| association between azotobacter and other soil bacteria and its effect on nitrogen fixation. | the association between a. vinelandii and either agrobacterium sp. or micrococcus sp., which are usually found as contaminants in azotobacter cultures, was investigated. in comparison with pure cultures, association increased the microbiol counts in addition to increasing nitrogen fixation rates and efficiency. in liquid cultures higher azotobacter densities were observed in the top 5 cm of the column concomitant with lowering the economic coefficient of utilization of carbonaceous compounds, wh ... | 1979 | 543344 |
| [the spreading of agrobacterium strains in soft-agar (author's transl)]. | in a diluted synthetic medium solidified by 0.17% agar populations of agrobacterium spread with a velocity of 0 to 20 mm/24 hours. this spreading is a consequence of chemotactic movement and cell division. different bacterial strains spread with a characteristic velocity each. loss of the ti-plasmid leaves the spreading behaviour unimpaired. it is changed after the introduction of virulence by kerr transfer or after prolonged culture in d-amino acids. | 1979 | 543348 |
| a study of the tumorogenesis of agrobacterium tumefaciens conn. on some plant species of iran. | this paper is a survey and comparative type of study on the tumorogenetic effect of agrobacterium tumefaciens on some plant species of iran. the results obtained indicate that the tumor-inducing property of this micro-organism is not common to all plant species studied; moreover no tumor was initiated on the mature leaves by the bacteria. the results obtained with the local varieties of the susceptible plants show some qualitative and quantitative differences as compared with the works of other ... | 1979 | 545942 |
| crown gall: economic importance and control. | many plants of economic importance are possible hosts for agrobacterium tumefaciens, the causal organism of the crown gall tumor disease. damage has been reported on stone fruit (australia), vineyard (hungary, bulgaria), lettuce (brasil) ... in western-europe, crown gall seems to be of less economic importance for plants growing in the open air. however, plants cultivated in greenhouses have a greater chance to be tumorized, because of the more favourable circumstances for tumor induction. moder ... | 1979 | 549390 |
| intra- and intergeneric similarities of the rrna cistrons of agrobacterium and their significance for the taxonomy of this genus [proceedings]. | | 1978 | 655694 |
| bacterial contamination of cotton and cotton dust and effects on the lung. | bacterial contamination of various parts of the cotton plant and of cotton from different mills was investigated. the predominant bacterial species were gram-negative rods mainly of the enterobacter genus. when guinea pigs inhaled strains of these bacteria cultivated from cotton, a strong leucocyte mobilising capacity was found for pseudomonas and enterobacter but not for agrobacterium or bacillus species. the aetiology of the development of pulmonary symptoms after inhalation of bacteria-contai ... | 1978 | 698133 |
| coordinated regulation of octopine degradation and conjugative transfer of ti plasmids in agrobacterium tumefaciens: evidence for a common regulatory gene and separate operons. | by using the analog noroctopine, mutants of agrobacterium tumefaciens were isolated with altered regulation patterns for the ti plasmid-borne octopine utilization genes. these could be divided into three classes: (i) strains with a constitutive level of octopine enzymes and a high degree of spontaneous ti transfer; (ii) one strain with constitutive octopine enzymes but no spontaneous ti transfer; and (iii) strains with an altered inducibility in which, contrary to the wild-type ti plasmid, conju ... | 1978 | 711678 |
| [ring structures on the cell wall of agrobacterium tumefaciens (author's transl)]. | | 1978 | 713041 |
| tumor-inducing (ti) plasmids of agrobacterium share extensive regions of dna homology. | labeled ti plasmid dnas from diverse agrobacterium strains were hybridized to southern blots of pti-b6-806 plasmid dna digest fragments of known map order. the map location of dna sequences common to all ti plasmids was found to be extensive, consistent with the view that ti plasmids have evolved from a common ancestral plasmid. | 1978 | 721776 |
| multiple genes coding for octopine-degrading enzymes in agrobacterium. | most biotype 2 strains of agrobacterium tumefaciens and a. radiobacter which utilize nopaline also degrade octopine. in all such strains studied, the ability to degrade octopine did not appear to be transferred to plasmidless recipient cells under conditions of plasmid transfer in which the ability to utilize nopaline was transferred. an octopine-degrading mutant was isolated in a strain cured of its plasmid, suggesting that genes of octopine degradation may have a chromosomal location in some s ... | 1978 | 721779 |
| [proliferative activity of normal and tumorous plant tissues cultivated in vitro]. | proliferative pools of helianthus tuberosus l. explants tissues were studied using autoradiography with 3h-thymidine in the course of normal growth in vitro and tumorous growth induced by agrobacterium tumefaciens. it is determined that on the 3d or 4th day of tumorous transformation the number of cycling cells reaches 30% whereas that of normally growing explants showed 10-11%. no changes were observed in 3h-thymidine transport during this period. | 1978 | 726052 |
| plasmid-dependent attachment of agrobacterium tumefaciens to plant tissue culture cells. | kinetic, microscopic, and biochemical studies show that virulent ti (tumor inducing)-plasmid-containing strains of agrobacterium attach to normal tobacco and carrot tissue culture cells. kinetic studies showed that virulent strains of a. tumefaciens attach to the plant tissue culture cells in increasing numbers during the first 1 to 2 h of incubation of the bacteria with the plant cells. five ti-plasmid-containing virulent agrobacterium strains showed greater attachment to tobacco cells than did ... | 1978 | 730370 |
| mutagenesis by insertion of the drug resistance transposon tn7 applied to the ti plasmid of agrobacterium tumefaciens. | | 1978 | 748948 |
| fingerprints of agrobacterium ti plasmids. | | 1978 | 748949 |
| restriction endonuclease mapping of a plasmid that confers oncogenicity upon agrobacterium tumefaciens strain b6-806. | | 1978 | 748950 |
| production of adventitious root primordia on hypocotyls of castor bean seedling, infected with agrobacterium tumefaciens. | the development of sterile secondary tumours on hypocotyls of castor bean seedlings, inoculated with any of the ten isolates of agrobacterium tumefaciens tested, were observed below the site of the primary tumours. histopathological studies performed in the present work indicate that the observed secondary tumours were adventitious root primordia and not the ordinary type of secondary tumours. according to the available literature such findings are reported here for the first time. all the teste ... | 1978 | 749411 |
| transfer, maintenance, and expression of bacterial ti-plasmid dna in plant cells transformed with a. tumefaciens. | the mechanism of induction of the plant cancer crown gall by agrobacterium tumefaciens has been briefly described. the salient points are as follows. 1. large plasmids of molecular weight (100 to 150) s 10(6), called ti-plasmids, are essential to the transformation process. 2. ti-plasmids carry a dna segment that can be transferred to, and maintained and expressed in, transformed plant cells. 3. this dna segment has been identified both by direct hybridization experiments between ti dna fragment ... | 1977 | 754869 |
| effect of sublethal heat injury on tumour induction and rna synthesis in agrobacterium tumefaciens. | when cells of agrobacterium tumefaciens are subjected to sublethal heat injury at 45 degrees c for 20 min, less than 5% of the viable population retain their ability to initiate tumour formation on kalanchöe daigremontiana. if the cells are then incubated in phosphate buffer at 27 degrees c for 2 h, tumour initiation returns to control levels. inhibitors of dna and protein synthesis had little effect on the recovery of tumour initiation after heat injury. rifamycin, a specific inhibitor of rna s ... | 1978 | 756950 |
| low-intensity microwave radiation and the virulence of agrobacterium tumefaciens strain b6. | when virulent cells of agrobacterium tumefaciens strain b6 were exposed to low-level microwave radiation at a frequency of 10,000 mhz and an intensity of 0.58 mw/cm2 for 30 to 120 min, a 30 to 60% decrease in their ability to produce tumors on potato and turnip disks was observed. this microwave exposure did not affect the viability of these bacteria or their ability to attach to a tumor-binding site nor did it induce thermal shock. this loss of virulence was reversible within 12 h. | 1979 | 760631 |
| conjugation in agrobacterium tumefaciens in the absence of plant tissue. | a general, reliable conjugation system for agrobacterium tumefaciens in the absence of plant tissue is described in which a. tumefaciens can serve either as the donor or recipient of plasmid deoxyribonucleic acid with reasonable efficiency. plasmid rp4 was transferred from escherichia coli to a. tumefaciens and from strain of a. tumefaciens. both rp4 and the a. tumefaciens virulence-associated plasmids were detected by alkaline sucrose gradients in a. tumefaciens strains a6 and c58 after mating ... | 1976 | 783141 |
| butandioldehydrogenase in eikenella corrodens and related bacteria. | the butandioldehydrogenase was examined in 153 strains of eikenella, moraxella, acinetobacter, agrobacterium, haemophilus, actinobacillus, pasteurella, cardiobacterium and "tm-1" of hollis et al. this enzyme has been proved to be useful in the differentiation of this group of bacteria. | 1976 | 797181 |
| particular small size rna and rna fragments from different origins as tumor inducing agents in datura stramonium. | particular rna fragments obtained by action of pancreatic ribonuclease on purified rnas originating from species totally unrelated to agrobacterium tumefaciens (escherichia coli, rabbit, monkey) are capable of inducing the formation of transplantable tumorous tissue when introduced at wounded sites in inverted stems of datura stramonium maintained under axenic conditions on a medium containing auxin and kinetin. reovirus rna and a small size rna (5-6s) isolated from rna bound rna directed dna po ... | 1976 | 822281 |
| octopine and nopaline metabolism in agrobacterium tumefaciens and crown gall tumor cells: role of plasmid genes. | crown gall tumors produced octopine or nopaline or neither compound, depending on the bacterial strain that incited the tumor. the genes specifying production of octopine or nopaline by the tumor were transferred to recipient bacterial strains when the large plasmid associated with virulence was transferred by either conjugation or deoxyribonucleic acid-mediated transformation. our results, which confirm the work of others (bomhoff et al., 1976; goldman et al., 1968; petit et al., 1970), indicat ... | 1977 | 830636 |
| plasmids in avirulent strains of agrobacterium. | twelve strains of agrobacterium radiobacter isolated from naturally occurring crown galls or soil were found to be avirulent on sunflower, tomato, kalanchoe, and carrot. eleven strains contained plasmids of molecular weights 77 x 10(6) to 182 x 10(6) as determined by electron microscopy. one strain contained only a smaller plasmid (50 x 10(6) daltons). several strains had both large and small (ca. 11 x 10(6) daltons) plasmids; one strain contained two large plasmids (112 x 10(6) and 136 x 10(6) ... | 1977 | 830650 |
| transfer of virulence in vivo and in vitro in agrobacterium. | | 1977 | 834308 |
| ti plasmids of agrobacterium as conjugative plasmids. | | 1977 | 834309 |
| induction of d-aldohexoside:cytochrome c oxidoreductase in agrobacterium tumefaciens. | d-aldohexopyranoside:cytochrome c oxidoreductase (aco) was strongly induced by cellobiose, alpha-methylglucoside, beta-methylglucoside, kojibiose, and sophorose. induction was rapid, and aco was readily detectable within 10 min after addition of cellobiose as inducer. although not measurable for 30 to 40 min after addition of inducer, once started, the rate of induction with alpha-methylglucoside equaled or even exceeded that obtained with cellobiose. induction by sucrose, maltose, alpha-alpha-t ... | 1977 | 838689 |
| comparison of thirty-seven strains of vd-3 bacteria with agrobacterium radiobacter: morphological and physiological observations. | thirty-seven cultures of vd-3 bacteria, isolated from clinical specimens, were characterized morphologically and physiologically. the cultures produced positive reactions when tested for oxidase, urease, nitrate reduction, phenylalanine deaminase, oxidative metabolism of carbohydrate substrates, and 3-ketolactose production. these peritrichously flagellated microorganisms were isolated primarily from the respiratory tract. when compared to authentic strains of agrobacterium, they appeared to be ... | 1977 | 845244 |
| [effect of inhibitors of transcription on the growth of mycoplasma and agrobacterium tumefaciens 8628]. | | 1977 | 865316 |
| [avirulent mutants of agrobacterium tumefaciens 8628 resistant to alpha-amanitin]. | | 1977 | 865330 |
| [content of nitrogen bases in the dna of carboxide bacteria]. | the composition of nitrogen bases was determined in total dna of live strains of carboxide bacteria belonging to the genera pseudomonas, comamonas, seliberia (agrobacterium), achromobacter. considerate differences in the nucleotide composition of dna of the studied bacteria confirm that they indeed belong to different genera. at the same time, the content of gc pairs in dna of these bacteria is within the range found for the corresponding genera by other authors. | 1977 | 882021 |
| zeatin ribonucleosides in the transfer ribonucleic acid of rhizobium leguminosarum, agrobacterium tumefaciens, corynebacterium fascians, and erwinia amylovora. | until recently, the presence in transfer ribonucleic acid (trna) of the hydroxylated cytokinin ribosylzeatin [n6-(4-hydroxy-3-methylbut-2-enyl)adenosine]was thought to be unique to higher plants. this extension of work from several laboratories indicates the presence of 2-methylthioribosylzeatin in the trna of the plant-associated bacteria rhizobium leguminosarum, agrobacterium tumefaciens, and corynebacterium fascians, but not in that of erwinia amylovora. this cytokinin has the cis configurati ... | 1977 | 893341 |
| a restriction endonuclease from agrobacterium tumefaciens. | | 1977 | 913578 |
| homology between ti-plasmids of agrobacterium tumefaciens: hybridzation studies using electron microscopy [proceedings]. | | 1977 | 913837 |
| comparative study of ti-plasmids in agrobacterium tumefaciens by use of restriction enzymes [proceedings]. | | 1977 | 913838 |
| [a study of six cultures of "agrobacterium tumefaciens" and "a. radiobacter" (author's transl)]. | | 1977 | 921138 |