| identification of clinical isolates of gram-negative nonfermentative bacteria by an automated cellular fatty acid identification system. | an automated cellular fatty acid (cfa) bacterial identification system, microbial identification system (mis; microbial id, newark, del.), was compared with a conventional system for the identification of 573 strains of gram-negative nonfermentative bacteria. mis identifications were based exclusively on the cfa composition following 22 to 26 h of growth at 28 degrees c on trypticase soy agar. mis identifications were listed with a confidence measurement (similarity index [si]) on a scale of 0 t ... | 1991 | 1774302 |
| isolation of pseudomonas pickettii strains that degrade 2,4,6-trichlorophenol and their dechlorination of chlorophenols. | three strains of pseudomonas pickettii that can grow with 2,4,6-trichlorophenol (2,4,6-tcp) as the sole source of carbon and energy were isolated from different mixed cultures of soil bacterial populations that had been acclimatized to 2,4,6-tcp. these strains released 3 mol of chloride ion from 1 mol of 2,4,6-tcp during the complete degradation of the tcp. of these strains, p. pickettii dtp0602 in high-cell-density suspension cultures dechlorinated various chlorophenols (cps). cells that were p ... | 1992 | 1599247 |
| [identification and typing of pseudomonas pickettii during an episode of nosocomial outbreak]. | from january to april 1989, pseudomonas pickettii was isolated from clinical specimens of 24 hospitalized patients at the national taiwan university hospital in taipei. the source of the organism was the 0.9% nacl solution prepared by the hospital pharmacy. a total of 39 isolates of p. pickettii were collected, including 28 from clinical specimens and 11 from 0.9% saline and distilled water during the outbreak. these microorganisms were studied by using four methods, namely, conventional biochem ... | 1992 | 1473371 |
| osteomyelitis and intervertebral discitis caused by pseudomonas pickettii. | pseudomonas pickettii, a nonfermenting, gram-negative rod, is rarely pathogenic. previous reports of infection with p. pickettii have largely involved direct contamination of supplies presumed to be sterile. we describe a case of vertebral osteomyelitis and intervertebral discitis caused by p. pickettii in a debilitated patient. the aggressive nature of this infection demonstrates that p. pickettii may be a more invasive organism than previously noted, particularly in hosts with weakened immunit ... | 1992 | 1401027 |
| complete nucleotide sequence of tbud, the gene encoding phenol/cresol hydroxylase from pseudomonas pickettii pko1, and functional analysis of the encoded enzyme. | the gene (tbud) encoding phenol hydroxylase, the enzyme that converts cresols or phenol to the corresponding catechols, has been cloned from pseudomonas pickettii pko1 as a 26.5-kbp bamhi-cleaved dna fragment, designated pro1957, which allowed the heterogenetic recipient pseudomonas aeruginosa pao1c to grow on phenol as the sole source of carbon. two subclones of pro1957 carried in trans have shown phenol hydroxylase activity in cell extracts of p. aeruginosa. the nucleotide sequence was determi ... | 1992 | 1400204 |
| a study of the va-1 group of pseudomonads and its relationship to pseudomonas pickettii. | the va-1 group of denitrifying pseudomonads was characterized and compared with pseudomonas pickettii (va-2). they share many features but differ in their production of acid from cellobiose, lactose and maltose and in their denitrification (gas from nitrate) at 35 degrees c. dna-dna hybridizations between a strain of va-1 and the type strain of p. pickettii disclosed 84% homology and hence indicated that va-1 is a biovar of this species. since both are potential human pathogens, features are pre ... | 1980 | 7229615 |
| proposal of burkholderia gen. nov. and transfer of seven species of the genus pseudomonas homology group ii to the new genus, with the type species burkholderia cepacia (palleroni and holmes 1981) comb. nov. | based on the 16s rrna sequences, dna-dna homology values, cellular lipid and fatty acid composition, and phenotypic characteristics, a new genus burkholderia is proposed for the rna homology group ii of genus pseudomonas. seven species in this group were transferred to the new genus. thus seven new combinations, burkholderia cepacia (palleroni and holmes 1981), burkholderia mallei (zopf 1885), burkholderia pseudomallei (whitmore 1913), burkholderia caryophylli (burkholder 1942), burkholderia gla ... | 1992 | 1283774 |
| recognition of pseudomonas pickettii in the clinical laboratory: biochemical characterization of 62 strains. | pseudomonas pickettii strains were studied to determine the characteristics essential for their identification in the clinical microbiology laboratory. preliminary investigations indicated that these glucose-oxidizing, denitrifying, gram-negative rods were quite similar to an unclassified group of clinical isolates designated va-2. gas liquid chromatography of trimethylsilyl derivatives of whole cell hydrolysates of p. pickettii and va-2 strains yielded nearly identical elution profiles. the va- ... | 1975 | 1176593 |
| a new denitrifying saprophyte related to pseudomonas pickettii. | | 1977 | 900697 |
| a taxonomic study of clinical isolates of pseudomonas pickettii, 'p. thomasii' and 'group ivd' bacteria. | on the basis of cluster analysis, average similarities within and between groups, and dna base composition of selected strains, pseudomonas pickettii appeared to be a distinct species comprising several biotypes. although some or all of these biotypes, represented by subclusters in our computer study, may ultimately warrant recognition as separate species, our results were not conclusive enough to warrant such a proposal at present. most strains tentatively named 'p. thomasii' could be included ... | 1979 | 521791 |
| a nosocomial common source outbreak caused by pseudomonas pickettii. | pseudomonas pickettii, a gram-negative bacillus which has been recovered rarely from clinical specimens, was isolated from the respiratory tracts of 9 of 29 (31%) pediatric intensive care unit patients. the reservoir of the organism was intrinsically contaminated single dose vials of tracheal irrigant solution. four additional hospitals in three other states have notified the centers for disease control of respiratory colonization with aerobic nonfermentative gram-negative organisms and have ass ... | 1984 | 6494013 |
| pseudomonas pickettii colonization associated with a contaminated respiratory therapy solution--illinois. | | 1983 | 6412062 |
| factors associated with pseudomonas pickettii intrinsic contamination of commercial respiratory therapy solutions marketed as sterile. | laboratory investigations were conducted to study the growth dynamics of pseudomonas pickettii in commercial 0.9% sodium chloride solution under various environmental conditions and to determine the retention of these organisms after challenge through a 0.2-micron cartridge filter system. low numbers of p. pickettii (1 to 10 cfu/ml of test solution) inoculated into commercial vials containing 5 ml of 0.9% sodium chloride solution and 500-ml volumes of 0.9% sodium chloride solution were shown to ... | 1985 | 4091563 |
| nosocomial pseudomonas pickettii colonization associated with a contaminated respiratory therapy solution in a special care nursery. | pseudomonas pickettii caused respiratory tract colonization in five infants in the special care nursery of a chicago hospital. all organisms had the same antimicrobial susceptibilities. endotracheal suctioning with saline from 5-ml unit-dose vials was identified by epidemiologic investigation as a risk factor for colonization. the vials of saline were contaminated with a strain of p. pickettii having the same antimicrobial susceptibility pattern as the isolates from patients. as part of an inves ... | 1985 | 4066922 |
| [pseudomonas pickettii septicemia]. | a case of bacteremia caused by pseudomonas pickettii in an insulin dependent diabetic patient is reported, the portal of entry was unknown. after a failure of cefotaxime therapy whereas the strain was found susceptible by disk assay to this antibiotic, the patient became afebrile with doxycycline treatment. the bacteriological findings as well as the clinical problems are reported in the present paper. | 1985 | 3975078 |
| pseudomonas pickettii as a cause of pseudobacteremia. | an outbreak of pseudobacteremia caused by pseudomonas pickettii biovariant 1 is reported. the common source was the aqueous chlorhexidine solution prepared by the hospital pharmacy. the contamination problem caused by the antiseptic solution was eventually solved by a series of preventive measures. | 1985 | 3973002 |
| oral succession of gram-negative bacilli in myelosuppressed cancer patients. | aerobic and facultative gram-negative bacilli (gnb) have been reported to increase on various body surfaces in the seriously ill and debilitated patient. this study examined quantitative aspects of gnb succession at five oral sites in cancer patients before and during myelosuppressive chemotherapy. gnb concentrations increased sharply during chemotherapy at 25 to 50% of the oral sites in both acute nonlymphocytic leukemia and small-cell lung carcinoma patients. most sites did not exhibit shifts ... | 1986 | 3018036 |
| typing of strains from a single-source outbreak of pseudomonas pickettii. | | 1994 | 8027332 |
| [nosocomial pseudomonas pickettii infection. danger of the use of ion-exchange resins]. | in a recent nosocomial outbreak of pseudomonas pickettii infection at the general hospital of corbeil-essonnes, france, two patients were moderately affected, but two others, who were diabetic, died of septicemia. the infection was transmitted by 0.05% aqueous solutions of chlorhexidine, the treatment of water for these solutions being the cause of contamination. the ion-exchange resin in the deionization cartridges allowed the surviving bacteria in the city water supply to grow while the bacter ... | 1987 | 2955360 |
| pneumonia caused by a newly recognized pseudomonad in a child with chronic granulomatous disease. | a pseudomonad was isolated from the pleural fluid and pulmonary decortication tissue of a 5-year-old child with chronic granulomatous disease. although the isolate was phenotypically similar to pseudomonas cepacia, its biochemical profile was more similar to that of pseudomonas pickettii biovar 2. its slow growth rate, ability to hydrolyze urea rapidly, and lateral and polar flagellar pattern were suggestive of oligella ureolytica (formerly cdc group ive). the cellular fatty acid composition was ... | 1990 | 2380349 |
| effect of disinfectants on pseudomonads colonized on the interior surface of pvc pipes. | we investigated the effect of disinfectants on microbial contamination present on the interior surface of polyvinyl chloride (pvc) pipes filled with 600 ml of water contaminated with pseudomonas pickettii and p. aeruginosa. after eight weeks, water was removed, and the test pipes exposed to various types of aqueous disinfectants. disinfectant samples were removed, neutralized, and examined for recovery of microorganisms by membrane filtration. after seven-days exposure, disinfectant solutions we ... | 1990 | 2293797 |
| an australia-wide epidemic of pseudomonas pickettii bacteraemia due to contaminated "sterile" water for injection. | nineteen cases of pseudomonas pickettii bacteraemia and one case of pseudomonas cepacia bacteraemia were identified in an australia-wide outbreak of nosocomial sepsis associated with contaminated water for injection. the contamination was limited to one batch of commercially produced water for injection. four different organisms were identified (three biotypes of p. pickettii and one of p. cepacia). however, p. pickettii biotype 1 appeared to be relatively more virulent than the other biotypes a ... | 1990 | 2198444 |
| molecular cloning, characterization, and regulation of a pseudomonas pickettii pko1 gene encoding phenol hydroxylase and expression of the gene in pseudomonas aeruginosa pao1c. | a 26-kilobase bamhi restriction endonuclease dna fragment was cloned from pseudomonas pickettii pko1, a strain isolated from a soil microcosm that had been amended with benzene, toluene, and xylene. this dna fragment, cloned into vector plasmid pro1727 and designated pro1957, allowed pseudomonas aeruginosa pao1c to grow on phenol as the sole source of carbon. physical and functional restriction endonuclease maps have been derived for the cloned dna fragment. two dna fragments carried in trans an ... | 1990 | 2115872 |
| genetic organization and regulation of a meta cleavage pathway for catechols produced from catabolism of toluene, benzene, phenol, and cresols by pseudomonas pickettii pko1. | plasmid pro1957 contains a 26.5-kb bamhi restriction endonuclease-cleaved dna fragment cloned from the chromosome of pseudomonas pickettii pko1 that allows p. aeruginosa pao1c to grow on toluene, benzene, phenol, or m-cresol as the sole carbon source. the genes encoding enzymes for meta cleavage of catechol or 3-methylcatechol, derived from catabolism of these substrates, were subcloned from pro1957 and were shown to be organized into a single operon with the promoter proximal to tbue. deletion ... | 1991 | 1856161 |
| [bacteriological studies on pharyngeal and tracheal colonization in patients of operations and patients in icu]. | bacterial colonization of the pharynx before and one week after operation, and contamination of the trachea during intubation in 7 patients who underwent operations were investigated. and bacterial colonization of pharynx, tracheas, and mechanical-ventilators in 7 icu patients was investigated. flora of palms of 10 icu personnel was also examined: 1) oral bacteria began to be found in the trachea several hours after intubation. 2) in oropharyngeal flora, one week after operation, neisseria and a ... | 1991 | 1783808 |
| biotransformation of nitrobenzene by bacteria containing toluene degradative pathways. | nonpolar nitroaromatic compounds have been considered resistant to attack by oxygenases because of the electron withdrawing properties of the nitro group. we have investigated the ability of seven bacterial strains containing toluene degradative pathways to oxidize nitrobenzene. cultures were induced with toluene vapor prior to incubation with nitrobenzene, and products were identified by high-performance liquid chromatography and gas chromatography-mass spectrometry. pseudomonas cepacia g4 and ... | 1991 | 1781679 |
| pseudomonas pickettii infections in a paediatric oncology unit. | over a 3-month period, seven patients in a paediatric oncology unit developed pseudomonas pickettii septicaemias. the outbreak was difficult to recognize since the cases occurred at widely spaced intervals and problems were experienced with the identification of the isolates. many of the isolates were initially misidentified on the basis of a short sugar set used in the laboratory for identification of the non-fermenting gram-negative bacilli. moreover, the organisms had varying sensitivity patt ... | 1991 | 1672323 |
| leads from the mmwr. pseudomonas pickettii colonization associated with contaminated respiratory therapy solution. | | 1983 | 6620494 |
| nosocomial infections by chlorhexidine solution contaminated with pseudomonas pickettii (biovar va-i). | over a period of 10 days, six patients in a cardiac intensive care unit developed pseudomonas pickettii (biovar va-i) septicaemia after installation of a venous catheter. the organism was also recovered from all the vials of the aqueous solution of 0.05 per cent chlorhexidine ('hibitane') prepared with contaminated bidistilled water. there were no further cases of infection when the use of this water was prohibited. | 1983 | 6663085 |
| pseudomonas pickettii bacteremia. | a case of bacteremia caused by pseudomonas pickettii is reported. this represents the first case of infection due to this species. | 1981 | 7229017 |
| the dynamic progression of evolved character states for aromatic amino acid biosynthesis in gram-negative bacteria. | a systematic analysis of the evolution of aromatic amino acid biosynthesis in the proteobacteria, previously focussed mainly upon the gamma subdivision, has now been extended to the beta subdivision. five lineages were studied, represented by neisseria gonorrhoeae, nitrosomonas europaea, alcaligenes faecalis, rrna group-iii pseudomonads/rubrivivax gelatinosus, and rrna group-ii pseudomonads/rhodocyclus tenuis. within the phenylalanine pathway, the bifunctional p-protein (chorismate mutase/prephe ... | 1994 | 7533594 |
| pyrimidine ribonucleoside catabolic enzyme activities of pseudomonas pickettii. | pyrimidine ribonucleoside catabolic enzyme activities of the opportunistic pathogen pseudomonas pickettii were examined. of the pyrimidine and related compounds tested, only dihydrouracil (nitrogen source) and ribose (carbon source) supported growth. thin-layer chromatographic separation of the uridine and cytidine catabolities produced by p. pickettii extracts indicated that this pseudomonad contained nucleoside hydrolase activity. its presence was confirmed by enzyme assay. hydrolase activity ... | 1994 | 7710277 |
| comparative ribosomal protein sequence analyses of a phylogenetically defined genus, pseudomonas, and its relatives. | i analyzed various families of ribosomal proteins obtained from selected species belonging to the genus pseudomonas sensu stricto and allied organisms which were previously classified in the genus pseudomonas. partial amino acid sequencing of l30 preparations revealed that the strains which i examined could be divided into three clusters. the first cluster, which was assigned to the genus pseudomonas sensu stricto, included pseudomonas aeruginosa, pseudomonas putida, pseudomonas mendocina, and p ... | 1995 | 7727274 |
| biochemical and molecular characterization of the pseudomonas lemoignei polyhydroxyalkanoate depolymerase system. | pseudomonas lemoignei has five different polyhydroxyalkanoate (pha) depolymerase genes (phaz1 to phaz5), which encode the extracellularly localized poly(3-hydroxybutyrate) (phb) depolymerases c, b, and d, poly(3-hydroxyvalerate) (phv) depolymerase, and phb depolymerase a, respectively. four of the five genes (phaz1 to phaz4) have been cloned, and one of them (phaz1) was studied in detail earlier (d. jendrossek, b. müller, and h. g. schlegel, eur. j. biochem. 218:701-710, 1993). the fifth pha dep ... | 1995 | 7836292 |
| sequence analysis of the gene cluster encoding toluene-3-monooxygenase from pseudomonas pickettii pko1. | the nucleotide (nt) sequence and gene organization of the locus encoding the initial step of the toluene-3-monooxygenase (tbu) pathway from pseudomonas pickettii pko1 has been determined. this is the first reported nt sequence for a toluene monooxygenase which hydroxylates the c-3 position of toluene. six tightly assembled structural genes encoding several tbu were identified and were designated tbua1, tbuu, tbub, tbuv, tbua2 and tbuc. comparison of the deduced amino acid (aa) sequences of each ... | 1995 | 7867951 |
| use of gene probes to aid in recovery and identification of functionally dominant 2,4-dichlorophenoxyacetic acid-degrading populations in soil. | the herbicide 2,4-dichlorophenoxyacetic acid (2,4-d) was applied to soils in microcosms, and degradation was monitored after each of five repeated additions. total dnas were isolated from soil bacterial communities after each 2,4-d treatment. the dna samples were analyzed on slot blots and southern blots by using a tfda gene probe subcloned from plasmid pjp4 and a spa probe derived from a different 2,4-d-degrading isolate, a sphingomonas paucimobilis strain. 2,4-d applied to soil was quickly deg ... | 1994 | 8017908 |
| analysis of competition in soil among 2,4-dichlorophenoxyacetic acid-degrading bacteria. | competition among indigenous and inoculated 2,4-dichlorophenoxyacetic acid (2,4-d)-degrading bacteria was studied in a native kansas prairie soil following 2,4-d additions. the soil was inoculated with four different 2,4-d-degrading strains at densities of 10(3) cells per g of soil; the organisms used were pseudomonas cepacia dbo1(pjp4) and three michigan soil isolates, strain 745, sphingomonas paucimobilis 1443, and pseudomonas pickettii 712. following 2,4-d additions, total soil dna was extrac ... | 1994 | 8017909 |
| integration and excision of a 2,4-dichlorophenoxyacetic acid-degradative plasmid in alcaligenes paradoxus and evidence of its natural intergeneric transfer. | a self-transmissible 2,4-dichlorophenoxyacetic acid (2,4-d)-degradative plasmid, pka2, has been identified in a new 2,4-d-degrading strain, alcaligenes paradoxus 2811p, isolated from agricultural soil. pka2 occurred as a 42.9-kb plasmid in strain 2811p. a derivative strain, 2811c, was isolated from a stock culture in which the entire pka2 plasmid was apparently integrated into the host chromosome without loss of the 2,4-d+ phenotype. this interpretation is based on the disappearance of a free pl ... | 1994 | 8071203 |
| a novel toluene-3-monooxygenase pathway cloned from pseudomonas pickettii pko1. | plasmid pro1957, which contains a 26.5-kb fragment from the chromosome of pseudomonas pickettii pko1, allows p. aeruginosa pao1 to grow on toluene or benzene as a sole carbon and energy source. a subclone of pro1957, designated pro1966, when present in p. aeruginosa pao1 grown in lactate-toluene medium, accumulates m-cresol in the medium, indicating that m-cresol is an intermediate of toluene catabolism. moreover, incubation of such cells in the presence of 18o2 followed by gas chromatography-ma ... | 1994 | 8206853 |
| typing of strains from a single-source outbreak of pseudomonas pickettii. | plasmid profiles, genome restriction fragment polymorphisms, carbohydrate oxidation-fermentation reactions, methylumbelliferyl substrate hydrolysis patterns, antimicrobial susceptibilities, and results obtained with the biolog gn biochemical substrate kit were used to type 19 common-source, but mixed-biotype, outbreak strains and one epidemiologically distinct strain of pseudomonas pickettii. biotyping with conventional and methylumbelliferyl substrates failed to distinguish between strains. pla ... | 1993 | 8263186 |
| comparison of biotyping, ribotyping, and pulsed-field gel electrophoresis for investigation of a common-source outbreak of burkholderia pickettii bacteremia. | over a 3-month period, six immunocompromised patients developed one or more episodes of burkholderia pickettii bacteremia and/or catheter infection. vials of a commercially available, "sterile" saline for injection which had been used for flushing the patients' indwelling intravenous devices were implicated as the common source of the organisms. no further cases were diagnosed once the use of this saline was discontinued. twenty-six isolates, including 9 outbreak-related strains from case patien ... | 1997 | 9163452 |
| infections caused by pseudomonas pickettii in association with permanent indwelling intravenous devices: four cases and a review. | permanent indwelling intravenous devices (piids) have become increasingly prevalent in the past decade. these devices offer the advantage of long-term and convenient venous access, but their not-infrequent colonization by bacterial or fungi can lead to bloodstream infection with or without sepsis. we describe a series of four patients with piids who became infected with pseudomonas pickettii, and we review the properties of this unusual organism and the clinical presentation of the infections it ... | 1993 | 8286629 |
| differentiation of pseudomonas solanacearum, pseudomonas syzygii, pseudomonas pickettii and the blood disease bacterium by partial 16s rrna sequencing: construction of oligonucleotide primers for sensitive detection by polymerase chain reaction. | the sequence of a 292 bp segment of the dna encoding 16s rrna (corresponding to positions 44-337 of the escherichia coli 16s rrna sequence) was determined for each of 40 pseudomonas solanacearum, four banana blood disease bacterium, three p. syzygii and two p. pickettii strains. phylogenetic relationships derived from comparison of these sequences to each other, and to equivalent 16s rrna gene sequences from other bacteria present in the embl databank, conform well with those obtained previously ... | 1993 | 8371118 |
| enzymatic degradation of poly(hydroxyalkanoates) by pseudomonas pickettii. | a bacterium capable of degrading poly(3-hydroxybutyrate) [p(3hb)] was isolated from laboratory media and identified as pseudomonas pickettii. the strain p. pickettii excreted an extracellular poly(hydroxyalkanoate) (pha) depolymerase and grew on p(3hb) as the sole carbon source. p. pickettii also grew on 3-hydroxybutyrate, glucose, fructose, citrate or succinate. however, only 3-hydroxybutyrate apart from p(3hb) induced the secretion of pha depolymerase. the pha depolymerase was purified from th ... | 1993 | 8373740 |
| cloning and characterization of the genes for p-nitrobenzoate degradation from pseudomonas pickettii yh105. | pseudomonas pickettii yh105 was isolated for its ability to utilize p-nitrobenzoate as the sole source of carbon, nitrogen, and energy. degradation of p-nitrobenzoate by this strain proceeds through a reductive route as evidenced by the accumulation of ammonia in the culture medium during growth on p-nitrobenzoate. enzyme assays and high-performance liquid chromatography (hplc) analysis of culture supernatants indicate that p-nitrobenzoate is degraded through p-hydroxylaminobenzoate and protocat ... | 1995 | 8534095 |
| transfer of two burkholderia and an alcaligenes species to ralstonia gen. nov.: proposal of ralstonia pickettii (ralston, palleroni and doudoroff 1973) comb. nov., ralstonia solanacearum (smith 1896) comb. nov. and ralstonia eutropha (davis 1969) comb. nov. | based on the results of phenotypic characterization, cellular lipid and fatty acid analysis, phylogenetic analysis of 16s rdna nucleotide sequences and rna-dna hybrization, burkholderia pickettii, burkholderia solanacearum and alcaligenes eutrophus are transferred to the new genus ralstonia, and ralstonia pickettii (ralston, palleroni and doudoroff 1973) comb. nov., ralstonia solanacearum (smith 1896) comb. nov., and r. eutropha (davis 1969) comb. nov. are proposed. the type species of the new g ... | 1995 | 8657018 |
| chloroform mineralization by toluene-oxidizing bacteria. | seven toluene-oxidizing bacterial strains (pseudomonas mendocina kr1, burkholderia cepacia g4, pseudomonas putida f1, pseudomonas pickettii pko1, and pseudomonas sp. strains envpc5, envbf1, and env113) were tested for their ability to degrade chloroform (cf). the greatest rate of cf oxidation was achieved with strain envbf1 (1.9 nmol/min/mg of cell protein). cf also was oxidized by p. mendocina kr1 (0.48 nmol/min/mg of cell protein), strain envpc5 (0.49 nmol/min/mg of cell protein), and escheric ... | 1996 | 8702263 |
| a novel phenylserine dehydratase from pseudomonas pickettii ps22: purification, characterization, and sequence of its phosphopyridoxyl peptide. | a novel phenylserine dehydratase [ec 4.2.1.-], which catalyzes the deamination of l-threo-3-phenylserine to yield phenylpyruvate and ammonia, was purified to homogeneity from a crude extract of pseudomonas pickettii ps22 isolated from soil. the enzyme was a monomer having a molecular mass of about 38 kda and contained 1 mol of pyridoxal 5'-phosphate per mol of enzyme. the enzyme exhibited absorption maxima at 279 and 416 nm. no appreciable spectral change was observed over the ph range of 6.0 to ... | 1996 | 8743570 |
| pseudomonas pickettii pneumonia in a diabetic patient. | pseudomonas pickettii is a nonfermenting gram negative rod closely related to pseudomonas aeruginosa that rarely causes human disease. we describe a case of p pickettii pneumonia in a 41-year-old diabetic patient. two months prior to admission, patient was treated for a methicillin resistant staphylococcus aureus pneumonia. present illness started 2 days prior to admission with fever, chills, pleuritic chest pain, and productive cough. chest x-ray showed a right lower lobe infiltrate with effusi ... | 1995 | 8778213 |
| nosocomial outbreak of burkholderia pickettii infection due to a manufactured intravenous product used in three hospitals. | forty-six cases of nosocomial infection caused by burkholderia pickettii were reported between june and november 1993 in three metropolitan hospitals in madrid. a case-control study of the outbreak was conducted to identify its cause. seventy-four percent of the patients were males; the mean age +/- sd of the patients was 54 +/- 20 years. sixty-five percent of the patients presented with some gastrointestinal disorder, and 80% had a peripheral catheter; 98% were treated with intravenous fluids, ... | 1996 | 8783718 |
| constitutive expression of the cloned phenol hydroxylase gene(s) from alcaligenes eutrophus jmp134 and concomitant trichloroethylene oxidation. | given the demonstrated phenol-dependent trichloroethylene (tce) degradation in alcaligenes eutrophus jmp134 (a. r. harker and y. kim, appl. environ. microbiol. 56:1179-1181, 1990), this work represents a purposeful effort to create a constitutive degrader of tce. genes responsible for phenol hydroxylase activity were identified by tn5 transposon mutagenesis. mutants lacked both phenol hydroxylase and catechol 2,3-dioxygenase activities. southern blot analysis of total dna showed that all mutants ... | 1996 | 8795212 |
| nucleotide sequence analysis of genes encoding a toluene/benzene-2-monooxygenase from pseudomonas sp. strain js150. | it was previously shown by others that pseudomonas sp. strain js150 metabolizes benzene and alkyl- and chloro-substituted benzenes by using dioxygenase-initiated pathways coupled with multiple downstream metabolic pathways to accommodate catechol metabolism. by cloning genes encoding benzene-degradative enzymes, we found that strain js150 also carries genes for a toluene/benzene-2-monooxygenase. the gene cluster encoding a 2-monooxygenase and its cognate regulator was cloned from a plasmid carri ... | 1995 | 7574644 |
| aerobic degradation and dechlorination of 2-chlorophenol, 3-chlorophenol and 4-chlorophenol by a pseudomonas pickettii strain. | a gram-negative aerobic bacterium capable of using 2-chlorophenol (2-cp), 3-chlorophenol (3-cp) and 4-chlorophenol (4-cp) as sole carbon sources was isolated and characterized. the bacterium, designated ld1, was identified to be a pseudomonas pickettii strain. ld1 was able to totally degrade and dechlorinate 2-cp (initial concentration: 1.51 mmol l-1), 3-cp (initial concentration: 0.57 mmol l-1) and 4-cp (initial concentration: 0.75 mmol l-1) within 30, 30 and 40 h of incubation, respectively, u ... | 1995 | 7576526 |
| oligonucleotide primers designed to differentiate pathogenic pseudomonads on the basis of the sequencing of genes coding for 16s-23s rrna internal transcribed spacers. | universal primers targeting conserved sequences flanking the 3' end of the 16s and the 5' end of the 23s rrna genes (rdnas) were used to amplify the 16s-23s rdna internal transcribed spacers (its) from eight species of pseudomonads which have been associated with human infections. amplicons from reference strains of pseudomonas aeruginosa, pseudomonas cepacia, pseudomonas gladioli, pseudomonas mallei, pseudomonas mendocina, pseudomonas pickettii, pseudomonas pseudomallei, and xanthomonas maltoph ... | 1995 | 7583922 |
| comparison of factors influencing trichloroethylene degradation by toluene-oxidizing bacteria. | the degradation of trichloroethylene (tce) by toluene-oxidizing bacteria has been extensively studied, and yet the influence of environmental conditions and physiological characteristics of individual strains has received little attention. to consider these effects, the levels of tce degradation by strains distinguishable on the basis of toluene and nitrate metabolism were compared under aerobic or hypoxic conditions in the presence and absence of nitrate and an exogenous electron donor, lactate ... | 1996 | 8975612 |
| purification and characterization of 2,4,6-trichlorophenol-4-monooxygenase, a dehalogenating enzyme from azotobacter sp. strain gp1. | the enzyme which catalyzes the dehalogenation of 2,4,6-trichlorophenol (tcp) was purified to apparent homogeneity from an extract of tcp-induced cells of azotobacter sp. strain gp1. the initial step of tcp degradation in this bacterium is inducible by tcp; no activity was found in succinate-grown cells or in phenol-induced cells. nadh, flavin adenine dinucleotide, and o2 are required as cofactors. as reaction products, 2,6-dichlorohydroquinone and cl- ions were identified. studies of the stoichi ... | 1997 | 8981999 |
| effect of vitamins on the aerobic degradation of 2-chlorophenol, 4-chlorophenol, and 4-chlorobiphenyl. | the effect of vitamins on the aerobic degradation and dechlorination of 2-chlorophenol and 4-chlorophenol by pseudomonas pickettii, strain ld1, and 4-chlorobiphenyl by pseudomonas sp. strain cpe1 was determined. these microorganisms are capable of using the target compounds as the sole carbon and energy source, but do not need vitamins to metabolize them. the addition to the culture medium of a vitamin solution containing biotin, folic acid, pyridoxine hydrochloride, riboflavin, thiamine hydroch ... | 1996 | 8987730 |
| biodegradation of lantadene a, the pentacyclic triterpenoid hepatotoxin by pseudomonas pickettii. | a bacterial strain capable of biodegradation of lantadene a (22 beta-angeloyloxy-3-oxoolean-12-en-28-oic acid) has been isolated from soil using lantadene a as the sole carbon source. the organism is rod shaped, gram negative, motile and has been identified as pseudomonas pickettii. this is the first report of the biodegradation of a pentacyclic triterpenoid. | 1997 | 9134768 |
| plasmid-encoded sequestration of copper by pseudomonas pickettii strain us321. | pseudomonas pickettii strain us321 appeared to elicit a copper resistance mechanism upon exposure to copper. the bacterial colonies turned blue in the presence of this metal in a chemically defined medium, suggesting accumulation of copper. prolonged exposure to copper resulted in the characteristic "copper sink" morphology of the colonies. atomic absorption spectrophotometric analysis confirmed that this organism can accumulate copper. the strain us321 exhibited a high-molecular-weight plasmid, ... | 1997 | 9142746 |
| isolation of an acanthamoeba strain with intracellular burkholderia pickettii infection. | burkholderia pickettii is a facultative pathogen that has been isolated from patient sources and environmental sources including respiratory therapy solutions, deionized water and aqueous disinfectants. the organism has been associated with septicemia and respiratory tract infections. in our investigation, burkholderia pickettii (biovar 2) was for the first time isolated from acanthamoeba sp. (group ii), a free living amoeba species recovered from the wet area of a physiotherapy unit. pathogenic ... | 1997 | 9144916 |
| antibacterial activity of bms-180680, a new catechol-containing monobactam. | the in vitro activities of a new catechol-containing monobactam, bms-180680 (sq 84,100), were compared to those of aztreonam, ceftazidime, imipenem, piperacillin-tazobactam, ciprofloxacin, amikacin, and trimethoprim-sulfamethoxazole. bms-180680 was often the most active compound against many species of the family enterobacteriaceae, with mics at which 90% of the isolates were inhibited (mic90s) of < or = 0.5 microg/ml for escherichia coli, klebsiella spp., citrobacter diversus, enterobacter aero ... | 1997 | 9145861 |
| cross-regulation of toluene monooxygenases by the transcriptional activators tbmr and tbut. | the toluene-3-monooxygenase from burkholderia pickettii pko1 and the toluene/benzene-2-monooxygenase from burkholderia (pseudomonas) sp. strain js150 are distinct enzymes which differ not only in catalytic specificity and substrate range but also in the arrangement and sequence of the genes within the operons that encode the enzymes, tbua1ubva2c and tbmabcdef, respectively. in the present study, we examined the transcriptional activation of the ptbua1 and ptbma promoters by their cognate regulat ... | 1997 | 9293027 |
| repression of phenol catabolism by organic acids in ralstonia eutropha. | during batch growth of ralstonia eutropha (previously named alcaligenes eutrophus) on phenol in the presence of acetate, acetate was found to be the preferred substrate; this organic acid was rapidly metabolized, and the specific rate of phenol consumption was considerably decreased, although phenol consumption was not abolished. this decrease corresponded to a drop in phenol hydroxylase and catechol-2,3-dioxygenase specific activities, and the synthesis of the latter was repressed at the transc ... | 1998 | 9435054 |
| survival in soil of different toluene-degrading pseudomonas strains after solvent shock. | we assayed the tolerance to solvents of three toluene-degrading pseudomonas putida strains and pseudomonas mendocina kr1 in liquid and soil systems. p. putida dot-t1 tolerated concentrations of heptane, propylbenzene, octanol, and toluene of at least 10% (vol/vol), while p. putida f1 and eez15 grew well in the presence of 1% (vol/vol) propylbenzene or 10% (vol/vol) heptane, but not in the presence of similar concentrations of octanol or toluene. p. mendocina kr1 grew only in the presence of hept ... | 1998 | 9435060 |
| rhizoremediation of trichloroethylene by a recombinant, root-colonizing pseudomonas fluorescens strain expressing toluene ortho-monooxygenase constitutively. | trichloroethylene (tce) was removed from soils by using a wheat rhizosphere established by coating seeds with a recombinant, tce-degrading pseudomonas fluorescens strain that expresses the toma+ (toluene o-monooxygenase) genes from burkholderia cepacia pr1(23)(tom23c). a transposon integration vector was used to insert toma+ into the chromosome of p. fluorescens 2-79, producing a stable strain that expressed constitutively the monooxygenase at a level of 1.1 nmol/min.mg of protein (initial tce c ... | 1998 | 9435067 |
| cloning and sequence analysis of a catechol 2,3-dioxygenase gene from the nitrobenzene-degrading strain comamonas sp js765. | comamonas sp strain js765 utilizes nitrobenzene as a carbon and nitrogen source. the initial attack on nitrobenzene is carried out by nitrobenzene 1,2-dioxygenase, which converts nitrobenzene to an unstable nitrohydrodiol that spontaneously decomposes to form catechol and nitrite. catechol is then degraded via a meta cleavage pathway. we now report the cloning of a dna fragment carrying a catechol 2,3-dioxygenase gene from js765. nucleotide sequence analysis revealed three open reading frames (o ... | 1997 | 9451836 |
| microbial communities of printing paper machines. | the microbial content of printing paper machines, running at a temperature of 45-50 degrees c and at ph 4.5-5, was studied. bacteria were prevalent colonizers of the machine wet end and the raw materials. a total of 390 strains of aerobic bacteria were isolated and 86% of these were identified to genus and species by biochemical, chemotaxonomic and phylogenetic methods. the most common bacteria found at the machine wet end were bacillus coagulans and other bacillus species, burkholderia cepacia, ... | 1998 | 9717292 |
| outbreak of pseudomonas fluorescens bacteremia among oncology patients. | from 7 to 24 march 1997, four patients developed pseudomonas fluorescens bacteremia at the hospital; one on the oncology ward and the other three in the chemotherapy room. these patients all had underlying malignancies and had the port-a-cath (smiths industries medical systems, deltec, inc., st. paul, minn.) implants. three patients had primary bacteremia, and one had port-a-cath-related infection. none of these patients had received a blood transfusion before the episodes of bacteremia. all pat ... | 1998 | 9738043 |
| alcaligenes eutrophus as a bacterial chromate sensor. | in alcaligenes eutrophus ch34, determinants encoding inducible resistance to chromate (chr) and to cobalt and nickel (cnr) are located adjacent to each other on plasmid pmol28. to develop metal-sensing bacterial strains, a cloned part of plasmid pmol28, which contains both determinants, was mutated with tn5-lacz. the chr::lacz fusions were specifically induced by chromium; cnr was induced best by ni2+ but was also induced by co2+, mn2+, chromate, cu2+, cd2+, and zn2+. the broad-host-range incp1 ... | 1998 | 9464379 |
| evolutionary relationship between chlorocatechol catabolic enzymes from rhodococcus opacus 1cp and their counterparts in proteobacteria: sequence divergence and functional convergence. | biochemical investigations of the muconate and chloromuconate cycloisomerases from the chlorophenol-utilizing strain rhodococcus opacus (erythropolis) 1cp had previously indicated that the chlorocatechol catabolic pathway of this strain may have developed independently from the corresponding pathways of proteobacteria. to test this hypothesis, we cloned the chlorocatechol catabolic gene cluster of strain 1cp by using pcr with primers derived from sequences of n termini and peptides of purified c ... | 1998 | 9495745 |
| bacterial community dynamics during start-up of a trickle-bed bioreactor degrading aromatic compounds. | this study was performed with a laboratory-scale fixed-bed bioreactor degrading a mixture of aromatic compounds (solvesso100). the starter culture for the bioreactor was prepared in a fermentor with a wastewater sample of a care painting facility as the inoculum and solvesso100 as the sole carbon source. the bacterial community dynamics in the fermentor and the bioreactor were examined by a conventional isolation procedure and in situ hybridization with fluorescently labeled rrna-targeted oligon ... | 1998 | 9501433 |
| enzyme characteristics of beta-d-galactosidase- and beta-d-glucuronidase-positive bacteria and their interference in rapid methods for detection of waterborne coliforms and escherichia coli. | bacteria which were beta-d-galactosidase and beta-d-glucuronidase positive or expressed only one of these enzymes were isolated from environmental water samples. the enzymatic activity of these bacteria was measured in 25-min assays by using the fluorogenic substrates 4-methylumbelliferyl-beta-d-galactoside and 4-methylumbelliferyl-beta-d-glucuronide. the enzyme activity, enzyme induction, and enzyme temperature characteristics of target and nontarget bacteria in assays aimed at detecting colifo ... | 1998 | 9501441 |
| multiple beta-ketothiolases mediate poly(beta-hydroxyalkanoate) copolymer synthesis in ralstonia eutropha. | polyhydroxyalkanoates (phas) are a class of carbon and energy storage polymers produced by numerous bacteria in response to environmental limitation. the type of polymer produced depends on the carbon sources available, the flexibility of the organism's intermediary metabolism, and the substrate specificity of the pha biosynthetic enzymes. ralstonia eutropha produces both the homopolymer poly-beta-hydroxybutyrate (phb) and, when provided with the appropriate substrate, the copolymer poly(beta-hy ... | 1998 | 9555876 |
| ntn genes determining the early steps in the divergent catabolism of 4-nitrotoluene and toluene in pseudomonas sp. strain tw3. | pseudomonas sp. strain tw3 is able to oxidatively metabolize 4-nitrotoluene and toluene via a route analogous to the upper pathway of the tol plasmids. we report the sequence and organization of five genes, ntnwcmab*, which are very similar to and in the same order as the xyl operon of tol plasmid pww0 and present evidence that they encode enzymes which are expressed during growth on both 4-nitrotoluene and toluene and are responsible for their oxidation to 4-nitrobenzoate and benzoate, respecti ... | 1998 | 9555884 |
| bacterial contamination of commercially available ethacridine lactate (acrinol) products. | bacterial contamination of commercially available ethacridine lactate (acrinol) solutions and cotton gauze soaked in ethacridine lactate solution was investigated. of 56 samples from ethacridine lactate solutions (eight products, seven manufacturers), seven samples (12.5%) of two products (two manufacturers) were contaminated with 10(1)-10(4) colony forming units (cfu)/ml of burkholderia pickettii. of 67 samples obtained from gauze soaked in ethacridine lactate solution (seven products, seven ma ... | 1996 | 8880550 |
| sequence diversity of the opri gene, coding for major outer membrane lipoprotein i, among rrna group i pseudomonads. | the sequence of opri, the gene coding for the major outer membrane lipoprotein i, was determined by pcr sequencing for representatives of 17 species of rrna group i pseudomonads, with a special emphasis on pseudomonas aeruginosa and pseudomonas fluorescens. within the p. aeruginosa species, opri sequences for 25 independent isolates were found to be identical, except for one silent substitution at position 96. the opri sequences diverged more for the other rrna group i pseudomonads (85 to 91% si ... | 1998 | 9851998 |
| an outbreak of pseudobacteraemia caused by burkholderia pickettii: the critical role of an epidemiological link. | a protracted outbreak of pseudobacteraemia caused by burkholderia pickettii is reported. initial investigation did not reveal the source of the outbreak. subsequently, careful examination of patient records provided an insight to the probable source, which was further confirmed by a case control study. it was found that 48% of cases had either hepatobiliary diseases or haematological malignancies, whereas only 15% of the controls had similar problems. furthermore, a coagulation study was perform ... | 1996 | 8880551 |
| cascade regulation of the toluene-3-monooxygenase operon (tbua1ubva2c) of burkholderia pickettii pko1: role of the tbua1 promoter (ptbua1) in the expression of its cognate activator, tbut. | burkholderia pickettii pko1 metabolizes toluene and benzene via a chromosomally encoded toluene-3-monooxygenase pathway. expression of the toluene-3-monooxygenase operon (tbua1ubva2c) is activated by the regulator, tbut, in the presence of toluene. we have identified the tbut coding region downstream of the toluene-3-monooxygenase structural genes by nucleotide sequence analysis and have shown that although tbut is similar to xylr and dmpr, two members of the ntrc family of transcriptional activ ... | 1996 | 8892837 |
| genes for 2,4,5-trichlorophenoxyacetic acid metabolism in burkholderia cepacia ac1100: characterization of the tftc and tftd genes and locations of the tft operons on multiple replicons. | burkholderia cepacia ac1100 uses the chlorinated aromatic compound 2, 4,5-trichlorophenoxyacetic acid (2,4,5-t) as a sole source of carbon and energy. the enzyme which converts the first intermediate in the pathway, 2,4,5-trichlorophenol, to 5-chlorohydroquinone has been purified and consists of two subunits of 58 and 22 kda, encoded by the tftc and tftd genes (48). a degenerate primer was designed from the n terminus of the 58-kda polypeptide and used to isolate a clone containing the tftc and ... | 1998 | 9603818 |
| from the centers for disease control and prevention. nosocomial ralstonia pickettii colonization associated with intrinsically contaminated saline solution--los angeles, california, 1998. | | 1998 | 9634245 |
| isolation and characterization of phenol-degrading denitrifying bacteria. | phenol is a man-made as well as a naturally occurring aromatic compound and an important intermediate in the biodegradation of natural and industrial aromatic compounds. whereas many microorganisms that are capable of aerobic phenol degradation have been isolated, only a few phenol-degrading anaerobic organisms have been described to date. in this study, three novel nitrate-reducing microorganisms that are capable of using phenol as a sole source of carbon were isolated and characterized. phenol ... | 1998 | 9647811 |
| construction and use of an ipb dna module to generate pseudomonas strains with constitutive trichloroethene and isopropylbenzene oxidation activity. | pseudomonas sp. strain jr1 exhibits trichloroethene (tce) oxidation activity with isopropylbenzene (ipb) as the inducer substrate. we previously reported the genes encoding the first three enzymes of the ipb-degradative pathway (ipba1, ipba2, ipba3, ipba4, ipbb, and ipbc) and identified the initial ipb dioxygenase (ipba1 a2a3a4) as responsible for tce cooxidation (u. pflugmacher, b. averhoff, and g. gottschalk, appl. environ. microbiol. 62:3967-3977, 1996). primer extension analyses revealed mul ... | 1998 | 9647815 |
| a highly selective pcr protocol for detecting 16s rrna genes of the genus pseudomonas (sensu stricto) in environmental samples. | pseudomonas species are plant, animal, and human pathogens; exhibit plant pathogen-suppressing properties useful in biological control; or express metabolic versatilities valued in biotechnology and bioremediation. specific detection of pseudomonas species in the environment may help us gain a more complete understanding of the ecological significance of these microorganisms. the objective of this study was to develop a pcr protocol for selective detection of pseudomonas (sensu stricto) in envir ... | 1998 | 9647828 |
| in vitro antibacterial properties of pexiganan, an analog of magainin. | pexiganan, a 22-amino-acid antimicrobial peptide, is an analog of the magainin peptides isolated from the skin of the african clawed frog. pexiganan exhibited in vitro broad-spectrum antibacterial activity when it was tested against 3,109 clinical isolates of gram-positive and gram-negative, anaerobic and aerobic bacteria. the pexiganan mic at which 90% of isolates are inhibited (mic90) was 32 micrograms/ml or less for staphylococcus spp., streptococcus spp., enterococcus faecium, corynebacteriu ... | 1999 | 10103181 |
| aerobic mineralization of 2,6-dichlorophenol by ralstonia sp. strain rk1. | a new aerobic bacterium was isolated from the sediment of a freshwater pond close to a contaminated site at amponville (france). it was enriched in a fixed-bed reactor fed with 2,6-dichlorophenol (2,6-dcp)as the sole carbon and energy source at ph 7.5 and room temperature. the degradation of 2,6-dcp followed monod kinetics at low initial concentrations. at concentrations above 300 microm (50 mg.liter-1), 2,6-dcp increasingly inhibited its own degradation. the base sequence of the 16s ribosomal d ... | 1998 | 9647831 |
| evaluation of the vitek 2 system for rapid identification of medically relevant gram-negative rods. | the new vitek 2 system (biomérieux) was evaluated at two independent sites with the identification card for gram-negative bacilli (id-gnb card). of the 845 strains tested, which represented 70 different taxa belonging to either the family enterobacteriaceae or the nonenteric bacilli, 716 (84.7%) were correctly identified at the species level. thirty-two (3.8%) additional strains were identified to the species level after the performance of simple, rapid manual tests (oxidase, hemolysis, indole r ... | 1998 | 9650942 |
| structure of the o-specific polysaccharide from burkholderia pickettii strain nctc 11149. | a polymeric fraction (the putative o antigen) has been isolated from the lipopolysaccharide of the type strain of burkholderia pickettii. the components of the polymer and their molar proportions were: l-rhamnose (3), 2-acetamido-2-deoxy-d-glucose (1), and o-acetyl (1). by means of nmr studies and chemical degradations, the basic repeating-unit of the polymer was identified as a linear tetrasaccharide of the structure shown. the o-acetyl group is probably located at position 2 of the 3-substitut ... | 1996 | 8901090 |
| a physical genome map of the burkholderia cepacia type strain. | burkholderia cepacia (basonym pseudomonas cepacia), the type species of the new genus burkholderia, is of interest, not only because of its broad catabolic capacity and its ability to antagonize soil-borne plant pathogens, but also because of its causative role in infections in man, which are particularly evident in patients with cystic fibrosis. a physical map of the 8.1 mb genome of the b. cepacia type-strain atcc 25416 was constructed by applying two-dimensional pulsed-field gel electrophores ... | 1995 | 7476209 |
| nosocomial pseudomonas pickettii bacteremias traced to narcotic tampering. a case for selective drug screening of health care personnel. | three patients in a university hospital developed nosocomial infusion-related pseudomonas pickettii bacteremia. investigation identified six additional patients who had received intravenous fluid contaminated by p pickettii but did not become ill. all nine patients had had surgery, and each of these patients but only nine of 19 operated-on control patients had received intravenous fentanyl citrate in the operating room; the mean dose given to the nine case patients was far greater than that give ... | 1991 | 1992211 |
| ralstonia pickettii involved in spinal osteitis in an immunocompetent adult. | | 1998 | 9661960 |
| oxidation of trichloroethylene, 1,1-dichloroethylene, and chloroform by toluene/o-xylene monooxygenase from pseudomonas stutzeri ox1. | toluene/o-xylene monooxygenase (tomo) from pseudomonas stutzeri ox1, which oxidizes toluene and o-xylene, was examined for its ability to degrade the environmental pollutants trichloroethylene (tce), 1, 1-dichloroethylene (1,1-dce), cis-1,2-dce, trans-1,2-dce, chloroform, dichloromethane, phenol, 2,4-dichlorophenol, 2,4,5-trichlorophenol, 2,4,6-trichlorophenol, 2,3,5,6-tetrachlorophenol, and 2,3,4,5, 6-pentachlorophenol. escherichia coli jm109 that expressed tomo from genes on plasmid pbz1260 un ... | 1998 | 9687467 |
| phenotypic and genotypic characterization of clinical strains of cdc group ivc-2. | cdc group ivc-2 is a gram-negative, oxidase-positive, nonfermentative bacillus that has been implicated in human infections, including septicemia and peritonitis. biochemically it most closely resembles bordetella bronchiseptica and alcaligenes sp. results of cellular fatty acid (cfa) and 16s rrna gene analysis were combined with biochemical data to assist in identification and classification. the predominant cfas were hexadecanoic acid (16:0), cis-9-hexadecanoic acid (16:1omega7c), cis-11-octad ... | 1998 | 9705403 |
| molecular procedure for rapid detection of burkholderia mallei and burkholderia pseudomallei. | a pcr procedure for the discrimination of burkholderia mallei and burkholderia pseudomallei was developed. it is based on the nucleotide difference t 2143 c (t versus c at position 2143) between b. mallei and b. pseudomallei detected in the 23s rdna sequences. in comparison with conventional methods the procedure allows more rapid identification at reduced risk for infection of laboratory personnel. | 1998 | 9705426 |
| discrimination of burkholderia gladioli from other burkholderia species detectable in cystic fibrosis patients by pcr. | a procedure for molecular identification of burkholderia gladioli is described. specific 16s and 23s rrna gene signature sequences were defined as primers for pcr. the method allows rapid and specific discrimination of b. gladioli from related species (b. cepacia, b. multivorans, b. vietnamiensis, b. mallei, b. pseudomallei, ralstonia pickettii, and r. eutropha) and should contribute to the clarification of its role as a human pathogen, e.g., in cystic fibrosis. | 1998 | 9705429 |
| analysis of the gene cluster encoding toluene/o-xylene monooxygenase from pseudomonas stutzeri ox1. | the toluene/o-xylene monooxygenase cloned from pseudomonas stutzeri ox1 displays a very broad range of substrates and a very peculiar regioselectivity, because it is able to hydroxylate more than one position on the aromatic ring of several hydrocarbons and phenols. the nucleotide sequence of the gene cluster coding for this enzymatic system has been determined. the sequence analysis revealed the presence of six open reading frames (orfs) homologous to other genes clustered in operons coding for ... | 1998 | 9758777 |
| plasmids responsible for horizontal transfer of naphthalene catabolism genes between bacteria at a coal tar-contaminated site are homologous to pdtg1 from pseudomonas putida ncib 9816-4 | the presence of a highly conserved nahac allele among phylogenetically diverse bacteria carrying naphthalene-catabolic plasmids provided evidence for in situ horizontal gene transfer at a coal tar-contaminated site (j. b. herrick, k. g. stuart-keil, w. c. ghiorse, and e. l. madsen, appl. environ. microbiol. 63:2330-2337, 1997). the objective of the present study was to identify and characterize the different-sized naphthalene-catabolic plasmids in order to determine the probable mechanism of hor ... | 1998 | 9758778 |
| pcr-based detection of the causal agent of watermark disease in willows (salix spp.) | the watermark disease, caused by brenneria salicis (formerly erwinia salicis), is of significant concern wherever tree-forming willows are grown or occur naturally. the movement of infected, asymptomatic cuttings is a major cause of pathogen dispersal. a reliable and sensitive diagnostic procedure is necessary for the safe movement of willow planting material. we derived primers from the nucleotide sequence of the 16s rrna gene of b. salicis for the development of a pcr to detect this pathogen. ... | 1998 | 9758827 |
| selection and characterization of microorganisms utilizing thaxtomin a, a phytotoxin produced by streptomyces scabies | thaxtomin a is the main phytotoxin produced by streptomyces scabies, a causal agent of potato scab. thaxtomin a is a yellow compound composed of 4-nitroindol-3-yl-containing 2,5-dioxopiperazine. a collection of nonpathogenic streptomycetes isolated from potato tubers and microorganisms recovered from a thaxtomin a solution were examined for the ability to grow in the presence of thaxtomin a as a sole carbon or nitrogen source. three bacterial isolates and two fungal isolates grew in thaxtomin a- ... | 1998 | 9797282 |
| whole-cell kinetics of trichloroethylene degradation by phenol hydroxylase in a ralstonia eutropha jmp134 derivative | the rate, progress, and limits of trichloroethylene (tce) degradation by ralstonia eutropha aek301/pyk3021 whole cells were examined in the absence of aromatic induction. at tce concentrations up to 800 &mgr;m, degradation rates were sustained until tce was no longer detectable. the ks and vmax for tce degradation by aek301/pyk3021 whole cells were determined to be 630 &mgr;m and 22.6 nmol/min/mg of total protein, respectively. the sustained linear rates of tce degradation by aek301/pyk3021 up t ... | 1998 | 9797289 |
| detection of ralstonia solanacearum, which causes brown rot of potato, by fluorescent in situ hybridization with 23s rrna-targeted probes. | during the past few years, ralstonia (pseudomonas) solanacearum race 3, biovar 2, was repeatedly found in potatoes in western europe. to detect this bacterium in potato tissue samples, we developed a method based on fluorescent in situ hybridization (fish). the nearly complete genes encoding 23s rrna of five r. solanacearum strains and one ralstonia pickettii strain were pcr amplified, sequenced, and analyzed by sequence alignment. this resulted in the construction of an unrooted tree and suppor ... | 1998 | 9797321 |