| purification and some properties of a novel maltohexaose-producing exo-amylase from aerobacter aerogenes. | maltohexaose producing amylase (ec 3.2.1.-) is the fourth known exo-amylase, the three previously known being glucoamylase, beta-amylase and pseudomonas stutzeri maltotetraose producing amylase. the enzyme after release from aerobacter aerogenes cells by 0.1% sodium lauryl sulfate extraction was purified by ammonium sulfate precipitation, deae-sephadex column chromatography and sephadex g-100 gel filtration to 80-fold of the original sodium lauryl sulfate extract activity, it gave a single band ... | 1975 | 1094 |
| n-nitrosamine formation by cultures of several microorganisms. | of 38 pure cultures of microorganisms tested, only one, pseudomonas stutzeri, was capable of forming dimethylnitrosamine from dimethylamine and nitrite during growth. resting cells of p. stutzeri, cryptococcus terreus, escherichia coli, and xanthomonas campestris formed dimethylnitrosamine, although no nitrosamine was found in growing cultures of the latter three organisms. no nitrosamine was produced by either growing cultures or resting-cell suspensions of pseudomonas fragi or proteus mirabili ... | 1976 | 7197 |
| the biochemical pathway for the breakdown of n4-ethyl-l-asparagine in the bacterium pseudomonas stutzeri. | n4-ethyl-l-u-14casparagine and l-u-14caspartate give identical metabolites, mainly intermediates of the tricarboxylic acid cycle and related amino acids, in whole cells of pseudomonas stutzeri. the labelled asparagine derivative is converted into 14c-aspartate by cell-free extracts, and this reaction, which has an optimum ph of 8.8 +/- 0.2, is neither inhibited by unlabelled asparagine nor enhanced by unlabelled 2-oxoglutarate. no labelled keto acid corresponding to n4-ethylasparagine was detect ... | 1978 | 25650 |
| reduction of nitrite to nitrous oxide by a cytoplasmic membrane fraction from the marine denitrifier pseudomonas perfectomarinus. | a cytoplasmic membrane fraction from the marine denitrifier pseudomonas perfectomarinus reduced nitrite to nitrous oxide in a stoichiometric reaction without nitric oxide as free intermediate. the membrane system had a specific requirement for fmn with nad(p)h as electron donors. other electron donors were ascorbate-reduced cytochrome c-551 or phenazine methosulfate. the membrane fraction contained tightly bound cytochrome cd which represented only a small portion of the total cytochrome cd of t ... | 1979 | 228713 |
| starch metabolism in pseudomonas stutzeri. i. studies on maltotetraose-forming amylase. | the extracellular maltotetraose-forming amylase of pseudomonas stutzeri was purified to homogeneity by a combination of affinity and hydroxyapatite chromatography. sodium dodecyl sulfate-gel electrophoresis indicated that the oligomeric enzyme contains two different subunits with molecular weights of 48 000 and 58 000. cross-linking studies using dimethyl suberimidate have demonstrated that the native enzyme consists of dimers. seven isozymes of the amylase have been identified after polyacrylam ... | 1979 | 365247 |
| parathion utilization by bacterial symbionts in a chemostat. | a continuous-culture device was used to select and enrich for microorganisms, from sewage and agricultural runoff, that were capable of using the organophosphorus insecticide parathion as a sole growth substrate. parathion was dissimilated by the highly acclimated symbiotic activities of pseudomonas stutzeri, which non-oxidatively and cometabolically hydrolyzed the parathion to ionic diethyl thiophosphate and p-nitrophenol, and p. aeruginosa, which utilized the p-nitrophenol as a sole carbon and ... | 1977 | 410368 |
| starch metabolism in pseudomonas stutzeri. ii. purification and properties of a dextrin glycosyl-transferase (d-enzyme) and amylomaltase. | amylomaltase and disproportionating enzyme (d-enzyme) were purified to homogeneity from cell-free extracts of pseudomonas stutzeri using a six-step procedure. the presence of both glycosyltransferases in the same organism has not been reported before. molecular weight determination by gel chromatography gave a value of 74,000 for the amylomaltase and 115 000 for the d-enzyme. two subunits of different molecular weight were found in each enzyme as proved by sodium dodecyl sulfate-gel electrophore ... | 1979 | 758954 |
| [microflora of active ooze participating in the decomposition of sulfanilic acid]. | microflora of domestic water can be a source of active ooze adapted to sulphanilic acid. adaptation of the microflora to sulphanilic acid at a concentration of 170-200 mg/l takes 6 to 8 days. the microflora of active ooze, immediately after adaptation, consists mainly of pseudomonas species, ps. denitrificans, ps. fluorescens, ps. striata, ps. putida, etc., and also of achromobacter stutzeri, achromobacter flavum, mycobacterium phlei, mycobacterium mucosum, bacillus mesentericus, bac. cereus, sa ... | 1975 | 808686 |
| susceptibility of nonfermentative gram-negative bacilli to tobramycin. | there has been increasing interest in the pathogenic role of nonfermentative gram-negative bacilli in human infections. except for pseudomonas aeruginosa, the susceptibility pattern of these organisms to tobramycin has not been evaluated thoroughly. the activity of tobramycin, as compared with that of gentamicin, was tested by the serial broth dilution technique against 178 isolates of nonfermentative gram-negative bacilli obtained from various sources. p. aeruginosa, pseudomonas stutzeri, acine ... | 1976 | 823278 |
| [study of 14 denitrifying soil bacteria of the "pseudomonas stutzeri" group isolated by enrichment culture in the presence of nitrous oxide (author's transl)]. | the strains were isolated from soil by enrichment in a liquid minimal medium containing ethanol, acetate, succinate, l-malate or tartrate, under an n2o atmosphere at 32 degrees c. all fourteen strains can use the following 25 sources of carbon and energy under aerobic conditions: glycerate, ethanol, propanol, acetate, butyrate, malonate, succinate, glutarate, sebacate, glycollate, l-lactate, d-lactate, l-malate, dl-3-hydroxybutyrate, pyruvate, fumarate, itaconate, mesaconate, crotonate, l-alpha- ... | 1977 | 869407 |
| numerically dominant denitrifying bacteria from world soils. | nineteen soils, three freshwater lake sediments, and oxidized poultry manure were examined to determine the dominant denitrifier populations. the samples, most shown or expected to support active denitrification, were from eight countries and included rice paddy, temperate agricultural, rain forest, organic, and waste-treated soils. over 1,500 organisms that could grow anaerobically on nitrate agar were isolated. after purification, 146 denitrifiers were obtained, as verified by production of n( ... | 1977 | 869539 |
| unusual cause of urinary tract infection by pseudomonas stutzeri in singapore. | | 1977 | 926404 |
| sodium-dependent growth and respiration of a nonhalophilic bacterium, pseudomonas stutzeri. | pseudomonas stutzeri (van niel strain) requires na+ for growth. its growth rate was a sigmoidal function of na+ concentration, being maximal and constant from 2 to 50 mm-na+, and half maximal at about 0-5 mm-na+. the relationship between cell concentration and na+ concentration was non-linear; cell concentration increased abruptly when na+ was greater than 0-3 mm. accumulation of na+ in the organism during growth was not detected. in the presence of k+, respiration was enhanced specifically by n ... | 1976 | 978178 |
| [enteric fever by pseudomonas stutzeri]. | | 1975 | 1178950 |
| blockage by acetylene of nitrous oxide reduction in pseudomonas perfectomarinus. | suspensions of denitrifying cells of pseudomonas perfectomarinus reduced nitrate and nitrate as expected to dinitrogen; but, in the presence of acetylene, nitrous oxide accumulated when nitrate or nitrate was reduced. when supplied at the outset in place of nitrate and nitrate, nitrous oxide was rapidly reduced to dinitrogen by cells incubated in anaerobic vessels in the absence of acetylene. in the presence of 0.01 atmospheres of acetylene, however, nitrous oxide was not reduced. ethylene was n ... | 1976 | 1267447 |
| pitting of agar surface by pseudomonas stutzeri. | a strain of pseudomonas stutzeri which exhibited pitting of agar surfaces is described. | 1976 | 1270598 |
| comparison of dentrification by paracoccus denitrificans, pseudomonas stutzeri and pseudomonas aeruginosa. | the course of denitrification of nitrate, nitrite and both compounds together by static cultures of paracoccus denitrificans, pseudomonas stutzeri and pseudomonas aeruginosa was studied. these strains represent three different types of denitrification: 1. reduction of nitrate to gaseous nitrogen without accumulation of nitrite (p. denitrificans); 2. partial accumulation of nitrite in growing cultures during reduction of nitrate to gaseous nitrogen (p. aeruginosa) and 3. two-phase denitrification ... | 1992 | 1284849 |
| derived amino acid sequences of the nosz gene (respiratory n2o reductase) from alcaligenes eutrophus, pseudomonas aeruginosa and pseudomonas stutzeri reveal potential copper-binding residues. implications for the cua site of n2o reductase and cytochrome-c oxidase. | the nosz genes encoding the multicopper enzyme nitrous oxide reductase of alcaligenes eutrophus h16 and the type strain of pseudomonas aeruginosa were cloned and sequenced for structural comparison of their gene products with the homologous product of the nosz gene from pseudomonas stutzeri [viebrock, a. & zumft, w. g. (1988) j. bacteriol. 170, 4658-4668] and the subunit ii of cytochrome-c oxidase (coii). both types of enzymes possess the cua binding site. the nosz genes were identified in cosmi ... | 1992 | 1324835 |
| investigation of the solution conformation of cytochrome c-551 from pseudomonas stutzeri. | 1h nmr spectroscopy and solution structure computations have been used to examine ferrocytochrome c-551 from pseudomonas stutzeri (atcc 17588). resonance assignments are proposed for all main-chain and most side-chain protons. distance constraints were determined on the basis of nuclear overhauser enhancements between pairs of protons. dihedral angle constraints were determined from estimates of scaler coupling constants. twenty-four structures were calculated by distance geometry and refined by ... | 1992 | 1327105 |
| ionization of the heme propionate substituents in pseudomonad cytochromes c-551. | the heme propionate substituents in pseudomonas cytochrome c-551 are partially buried by folds of polypeptide in the structure of the protein, and are involved in several hydrogen bonds. the ionization behavior of these groups has been of interest because the oxidation potential of the heme changes with ph in a manner that may parallel ionization of a propionate. the ionization pka's of these groups have been determined by following the nmr chemical shifts of nearby protons acting as probes of t ... | 1992 | 1327881 |
| dna fingerprints of pseudomonas spp. using rotating field electrophoresis. | rotating field electrophoresis (rfe) was applied to evaluate the usefulness of this technique for identification of several pseudomonas strains with suspected importance in deliberate releases. genomes of common wild-type or genetically modified strains of pseudomonas fluorescens, pseudomonas stutzeri, pseudomonas putida and pseudomonas aeruginosa were digested with rare-cutting restriction endonucleases and subjected to pulsed field gel electrophoresis. restrictions with spei or xbai produced 1 ... | 1992 | 1364137 |
| purification and characterization of two forms of maltotetraose-forming amylase from pseudomonas stutzeri. | pseudomonas stutzeri mo-19 produced two active forms of extracellular maltotetraose-forming amylase. both forms, g4-1 and g4-2, were purified to electrophoretic homogeneity. the molecular masses of g4(-1) and g4(-2) were 57 kd and 46 kd by sds-polyacrylamide gel electrophoresis, respectively. an identical n-terminal sequence up to 20 amino acid residues and similar amino acid compositions were obtained from both forms, but different c-terminal amino acids, leucine from g4(-1) and alanine from g4 ... | 1990 | 1368535 |
| high-frequency electroporation and maintenance of puc- and pbr-based cloning vectors in pseudomonas stutzeri. | a number of escherichia coli cloning vectors, based on cole1-like replicons, were shown to be maintained in pseudomonas stutzeri atcc 17588. a restrictionless mutant of p. stutzeri was isolated, and this strain was used to develop an efficient electroporation system. with the e. coli cloning vector phsg298, transformation frequencies of up to 2 x 10(7) transformants/micrograms dna were achieved. this frequency is comparable to that obtained for cacl2-mediated transformation of e. coli; thus, dir ... | 1992 | 1369188 |
| effect of storage time with different lens care systems on in-office hydrogel trial lens disinfection efficacy: a multi-center study. | a combined prospective and retrospective study was conducted to evaluate the efficacy of in-office disinfection methods for hydrogel trial contact lenses. two hundred and twenty-one trial contact lenses, disinfected by four different disinfection methods, were collected from seven study centers and cultured for microbial contamination after various storage periods. negative and positive control lenses were included as an additional center in this double-masked study. there was a significant diff ... | 1992 | 1437007 |
| a comparison of substrates for quantifying the signal from a nonradiolabeled dna probe. | a method for measuring the amount of a nonradiolabeled dna probe using four detection substrates is described. in preliminary experiments, digoxygenin-labeled dna was bound to neutral, nylon membranes and detected with anti-digoxygenin antibodies conjugated to alkaline phosphatase. four substrates [4-nitrophenyl phosphate, 4-methylumbelliferyl phosphate, attophos, and adamantyl 1, 2-dioxetane phosphate (amppd)] were assessed for use in a quantitative hybridization assay. only attophos and amppd ... | 1992 | 1443585 |
| phosphate starvation affects the synthesis of outer membrane proteins in thiobacillus ferrooxidans. | the outer membrane protein (omp40) component from the chemolithoautotrophic acidophilic thiobacillus ferrooxidans is apparently regulated by the external ph and the concentration of phosphorus. its amino-terminal sequence showed little identity with the escherichia coli ompc, ompf or phoe porins, but was 38.5% identical to the outer membrane channel-forming protein nosa from pseudomonas stutzeri, whose expression is also regulated environmentally. in addition, the partial amino acid sequence of ... | 1992 | 1459418 |
| interdependence of respiratory no reduction and nitrite reduction revealed by mutagenesis of nirq, a novel gene in the denitrification gene cluster of pseudomonas stutzeri. | an open reading frame, designated nirq, was identified upstream of nirs, the structural gene for the respiratory nitrite reductase of pseudomonas stutzeri zobell. its derived gene product (275 amino acids, m(r) = 30,554) shows similarity to the ntrc protein family of transcriptional activators. deletion-replacement mutagenesis of the nirq gene resulted in the simultaneous loss of nitrite reduction and no reduction in vivo. however, both reductases were still synthesized, with only nitrite reduct ... | 1992 | 1468562 |
| sensitivity of pseudomonas stutzeri to edta: effects of growth parameters and test conditions. | edta is highly toxic for pseudomonas stutzeri. the bactericidal effect is not significantly attenuated by treatment of the cells at 0 degrees c rather than 25 degrees c, nor by osmotic stabilisation of the treated cells. the few surviving cells are not genetically resistant, but resistance to edta (and to some cationic antibiotics) can be induced by growing organisms in magnesium-limited media. under these conditions, there is increased production of a protein of molecular mass about 20 kd. comp ... | 1992 | 1470050 |
| periplasmic location of nitrous oxide reductase and its apoform in denitrifying pseudomonas stutzeri. | immunogold labelling techniques on ultrathin sections of low temperature embedded cells yielded evidence for the periplasmic location of the respiratory enzymes n2o reductase and nitrite reductase (cytochrome cd1) in pseudomonas stutzeri strain zobell. cell fractionation by spheroplast preparation and two-dimensional electrophoresis showed the absence of a membrane association of these enzymes. immunocytochemical localization of n2o reductase in a mutant strain deficient in the chromophore of n2 ... | 1992 | 1510553 |
| isolation and characterization of a nitrite reductase gene and its use as a probe for denitrifying bacteria. | the dissimilatory nitrite reductase gene (nir) from denitrifying bacterium pseudomonas stutzeri jm300 was isolated and sequenced. in agreement with recent sequence information from another strain of p. stutzeri (strain zobell), strain jm300 nir is the first gene in an operon and is followed immediately by a gene which codes for a tetraheme protein; 2.5 kb downstream from the nitrite reductase carboxyl terminus is the cytochrome c551 gene. p. stutzeri jm300 nir is 67% homologous to p. aeruginosa ... | 1992 | 1539983 |
| the structural genes of the nitric oxide reductase complex from pseudomonas stutzeri are part of a 30-kilobase gene cluster for denitrification. | a gene cluster of 30 kilobases required for denitrification in pseudomonas stutzeri zobell was identified and mapped. it harbors genes necessary for the respiratory reduction of nitrite (nir genes), nitric oxide (nor genes), and nitrous oxide (nos genes). fifteen genes, 13 of which are transcribed in the same direction, have been located on a 56-kb bamhi fragment. they are arranged in three subclusters in the order nos-nir-nor. | 1992 | 1551856 |
| community-acquired pneumonia due to pseudomonas stutzeri. | | 1992 | 1562677 |
| metabolic pathways in paracoccus denitrificans and closely related bacteria in relation to the phylogeny of prokaryotes. | denitrification and methylotrophy in paracoccus denitrificans are discussed. the properties of the enzymes of denitrification: the nitrate-nitrite antiporter, nitrate reductase, nitrite reductase, nitric oxide reductase and nitrous oxide reductase are described. the genes for none of these proteins have yet been cloned and sequenced from p. denitrificans. a number of sequences are available for enzymes from escherichia coli, pseudomonas stutzeri and pseudomonas aeruginosa. it is concluded that p ... | 1992 | 1575465 |
| nosr, a membrane-bound regulatory component necessary for expression of nitrous oxide reductase in denitrifying pseudomonas stutzeri. | the regulatory element nosr was identified within the nos region of the denitrification gene cluster of pseudomonas stutzeri zobell (atcc 14405) and characterized. it is essential for expression of the n2o reductase encoded by nosz immediately downstream of nosr. the nosr region was initially identified by tn5 mutagenesis (w. g. zumft, k. döhler, and h. körner, j. bacteriol. 163:918-924, 1985). it consists of a single open reading frame of 2,172 nucleotides and has the coding capacity for an 81. ... | 1992 | 1644760 |
| nitric oxide reductase. purification from paracoccus denitrificans with use of a single column and some characteristics. | nitric oxide reductase was purified from paracoccus denitrificans very nearly to homogeneity by a simple method that involved the use of octyl glucoside to solubilize the enzyme from membranes and required a single hydroxyapatite column. the enzyme had specific activities of about 10 mumol no reduced x min-1 x mg-1 at ph 6.5 in an amperometric assay system using phenazine methosulfate/ascorbate as the reducing agent and about 22 mumol no reduced x min-1 x mg-1 at ph 5.0, which is the optimum ph. ... | 1991 | 1645715 |
| proton resonance assignments for pseudomonas aeruginosa ferrocytochrome c-551. | a comparison between two sets of resonance assignments for ferrocytochrome c-551 from pseudomonas aeruginosa reveals that major differences can be explained by ph effects. in turn, these reveal side chain protonation events in c-551 that markedly influence spectra. the behavior of resonances in a homologous protein from pseudomonas stutzeri help to clarify ambiguities in the p. aeruginosa case. a corrected and completed set of proline assignments is presented. labile side chain protons in residu ... | 1991 | 1648911 |
| a model of the copper centres of nitrous oxide reductase (pseudomonas stutzeri). evidence from optical, epr and mcd spectroscopy. | nitrous oxide reductase (n2or), pseudomonas stutzeri, catalyses the 2 electron reduction of nitrous oxide to di-nitrogen. the enzyme has 2 identical subunits (mr approximately 70,000) of known amino acid sequence and contains approximately 4 cu ions per subunit. by measurement of the optical absorption, electron paramagnetic resonance (epr) and low-temperature magnetic circular dichroism (mcd) spectra of the oxidised state, a semi-reduced form and the fully reduced state of the enzyme it is show ... | 1991 | 1660405 |
| in vivo expression of the pseudomonas stutzeri maltotetraose-forming amylase gene (amyp). | northern hybridization and s1 nuclease mapping revealed that the amyp gene coding for maltotetraose-forming amylase of pseudomonas stutzeri mo-19 is transcribed as a monocistronic mrna of 2.0 kilobases and that the transcription start site is located 81 base pairs upstream from the first nucleotide of the initiation codon. the amyp gene was expressed weakly in escherichia coli, and transcription started 49 base pairs downstream of the p. stutzeri mo-19 transcription start site. synthesis of the ... | 1990 | 1689717 |
| cyclohexadienyl dehydratase from pseudomonas aeruginosa. molecular cloning of the gene and characterization of the gene product. | the gene encoding cyclohexadienyl dehydratase (denoted phec) was cloned from pseudomonas aeruginosa by functional complementation of a phea auxotroph of escherichia coli. the gene was highly expressed in e. coli due to the use of the high-copy number vector puc18. the p. aeruginosa cyclohexadienyl dehydratase expressed in e. coli was purified to electrophoretic homogeneity. the latter enzyme exhibited identical physical and biochemical properties as those obtained for cyclohexadienyl dehydratase ... | 1992 | 1733946 |
| marker exchange of the structural genes for nitric oxide reductase blocks the denitrification pathway of pseudomonas stutzeri at nitric oxide. | bacterial denitrification reverses nitrogen fixation in the global n-cycle by transforming nitrate or nitrite to dinitrogen. both nitrite and nitric oxide (no) are considered as the chemical species within the denitrification pathway, that precede nitrous oxide (n2o), the first recognized intermediate with n,n-bonds antecedent to n2. molecular cloning of the structural genes for no reductase from pseudomonas stutzeri has allowed us to generate the first mutants defective in no utilization (nor- ... | 1991 | 1744072 |
| efficient biodegradation of high-molecular-weight polyethylene glycols by pure cultures of pseudomonas stutzeri. | biodegradation of polyethylene glycols (pegs) of up to 13,000 to 14,000 molecular weight has been shown to be performed by a river water bacterial isolate (strain ja1001) identified as pseudomonas stutzeri. a pure culture of strain ja1001 grew on peg 1000 or peg 10000 at 0.2% (wt/vol) as a sole source of carbon and energy with a doubling time of 135 or 150 min, respectively. cultures metabolized 2 g of polymer per liter in less than 24 h and 10 g/liter in less than 72 h. the limit of 13,500 mole ... | 1991 | 1768106 |
| extended x-ray absorption fine structure and electron paramagnetic resonance of nitrous oxide reductase from pseudomonas aeruginosa strain p2. | the copper centers of nitrous oxide reductase from pseudomonas aeruginosa strain p2 were studied by x-ray and electron paramagnetic resonance (epr) spectroscopy. the enzyme is dimeric and contains four cu atoms and about seven cysteine residues/subunit of mr = 73,000. the extended x-ray absorption fine structure (ex-afs) spectrum was analyzed for enzyme as isolated (oxidized or slightly reduced), enzyme exposed briefly to air, reduced enzyme, and enzyme at ph 7 after having been activated by sta ... | 1991 | 1846618 |
| cytochrome oxidase genes from thermus thermophilus. nucleotide sequence and analysis of the deduced primary structure of subunit iic of cytochrome caa3. | cytochrome caa3, a cytochrome c oxidase from thermus thermophilus, is a two-subunit enzyme containing the four canonical metal centers of cytochrome c oxidases (cytochromes a and a3; copper centers cua and cub) and an additional cytochrome c. the smaller subunit contains heme c and was termed the c-protein. we have cloned the genes encoding the subunits of the oxidase and determined the nucleotide sequence of the c-protein gene. the gene and deduced primary amino acid sequences establish that bo ... | 1991 | 1848234 |
| purification of cytochrome cd1 nitrite reductase from pseudomonas stutzeri jm300 and reconstitution with native and synthetic heme d1. | cytochrome cd1 nitrite reductase has been purified from pseudomonas stutzeri strain jm 300. this enzyme appears to be a dimer with a subunit molecular mass of 54 kda and its isoelectric point is determined to be 5.4. the n terminus of amino acid sequence has strong homology with that of nitrite reductase from p. aeruginosa. the apoprotein of this enzyme has been reconstituted with native and synthetic heme d1. the nitrite reductase activity measured by no and n2o gas evolution can be restored to ... | 1991 | 1850410 |
| molecular cloning and functional characterization of a reca analog from pseudomonas stutzeri and construction of a p. stutzeri reca mutant. | a recombinant plasmid carrying the pseudomonas stutzeri reca gene was isolated by complementation of the escherichia coli reca13 mutation. subcloning experiments showed that the gene was located on a 1500 bp pvuii-bglii fragment. the cloned gene complements an e. coli reca mutant for resistance to methylmethanosulphonate (mms) and uv irradiation. it was also capable of restoring the recombination proficiency in that mutant. the cloned fragment was used to construct a reca deletion mutant of p. s ... | 1991 | 1854185 |
| molecular characterization of nosa, a pseudomonas stutzeri gene encoding an outer membrane protein required to make copper-containing n2o reductase. | a pseudomonas stutzeri gene (nosa) encoding an outer membrane protein was cloned into the broad-host-range vector prk290 and expressed in a mutant lacking the protein. deletion analysis identified the approximate extent of the nosa region which was sequenced, and it was found to contain an open reading frame encoding 683 amino acids including a presumed signal sequence of 44 amino acids. the putative processed form had a molecular weight of 70,218, characteristics typical of outer membrane prote ... | 1991 | 1885521 |
| a novel lipopolysaccharide-binding hemagglutinin isolated from hemocytes of the solitary ascidian, halocynthia roretzi: it can agglutinate bacteria. | a hemagglutinin was isolated from hemocytes of the ascidian, halocynthia roretzi, by a procedure including extraction and ion-exchange chromatography on cm-cellulose. the molecular weight of the hemagglutinin was estimated to be 120,000 by gel filtration. it was resistant to acid treatment but sensitive to alkali or heat treatment. the hemagglutinating activity was inhibited by heparin, chondroitin sulfate, and lipopolysaccharide (lps), but not by mono- and disaccharides such as n-acetyl-galacto ... | 1991 | 1904830 |
| [lower respiratory tract infection by pseudomonas stutzeri]. | | 1991 | 1912161 |
| cloning, sequencing, and expression of the p-protein gene (phea) of pseudomonas stutzeri in escherichia coli: implications for evolutionary relationships in phenylalanine biosynthesis. | the phea gene encoding the bifunctional p-protein (chorismate mutase:prephenate dehydratase) was cloned from pseudomonas stutzeri and sequenced. this is the first gene of phenylalanine biosynthesis to be cloned and sequenced from pseudomonas. the phea gene was expressed in escherichia coli, allowing complementation of an e. coli phea auxotroph. the enzymic and physical properties of the p-protein from a recombinant e. coli auxotroph expressing the phea gene were identical to those of the native ... | 1991 | 1919506 |
| the copper site in nitrous oxide reductase. | the properties of the novel copper enzyme nitrous oxide reductase from denitrifying pseudomonas stutzeri are described. multifrequency electron paramagnetic resonance spectroscopy is used to characterize the various forms of the enzyme. the features observed at 2.4, 3.4, 4.5, 9.31 and 35 ghz are explained by a mixed-valence [cu(1.5)...cu(1.5)] s = 1/2 species with the unpaired electron delocalized between the two cu nuclei. this site is also present in the catalytically inactive derivative of ni ... | 1990 | 1965779 |
| the nirstbm region coding for cytochrome cd1-dependent nitrite respiration of pseudomonas stutzeri consists of a cluster of mono-, di-, and tetraheme proteins. | genes for respiratory nitrite reduction (denitrification) of pseudomonas stutzeri are clustered within 7 kbp. a 4.6-kbp hind iii-kpn i fragment carrying nirs, the structural gene for cytochrome cd1, was sequenced. an open reading frame immediately downstream of nirs codes for a 22.8-kda protein with four heme c-binding motifs. mutagenesis of this gene causes an apparent defect in electron donation to cytochrome cd1. following this orf are the structural genes for cytochrome c552, cytochrome c551 ... | 1991 | 2001732 |
| close linkage in pseudomonas stutzeri of the structural genes for respiratory nitrite reductase and nitrous oxide reductase, and other essential genes for denitrification. | the structural gene, nirs, for the respiratory nitrite reductase (cytochrome cd1) from pseudomonas stutzeri was identified by (i) sequencing of the n-terminus of the purified protein and partial sequencing of the cloned gene, (ii) immunoscreening of clones from a lambda gt11 expression library, (iii) mapping of the transposon tn5 insertion site in the nirs mutant strain mk202, and (iv) complementation of strain mk202 with a plasmid carrying the insert from an immunopositive lambda clone. a mutat ... | 1991 | 2005866 |
| catalysis of nitrosyl transfer by denitrifying bacteria is facilitated by nitric oxide. | two denitrifying bacteria, pseudomonas stutzeri and achromobacter cycloclastes, were incubated with na15no2 and nan3 under conditions that allowed catalysis of nitrosyl transfer from nitrite to azide. this transfer, which is presumed to be mediated by the heme- and copper-containing nitrite reductase of p. stutzeri and a. cycloclastes, respectively, leads to formation of isotopically mixed 14,15n2o, whereas denitrification leads to 15n2o. the conditions that emphasized nitrosyl transfer also par ... | 1991 | 2025262 |
| metabolic regulation including anaerobic metabolism in paracoccus denitrificans. | under anaerobic circumstances in the presence of nitrate paracoccus denitrificans is able to denitrify. the properties of the reductases involved in nitrate reductase, nitrite reductase, nitric oxide reductase, and nitrous oxide reductase are described. for that purpose not only the properties of the enzymes of p. denitrificans are considered but also those from escherichia coli, pseudomonas aeruginosa, and pseudomonas stutzeri. nitrate reductase consists of three subunits: the alpha subunit con ... | 1991 | 2050653 |
| exchange of chromosomal markers by natural transformation between the soil isolate, pseudomonas stutzeri jm300, and the marine isolate, pseudomonas stutzeri strain zobell. | both the soil isolate, pseudomonas stutzeri jm300, and the marine isolate, pseudomonas stutzeri strain zobell, have been shown previously to be naturally transformable. this study reports the detection of genetic exchange by natural transformation between these two isolates. transformation frequency was determined by filter transformation procedures. three independent antibiotic resistance loci were used as chromosomal markers to monitor this exchange event: resistance to rifampicin, streptomyci ... | 1991 | 2059008 |
| inhibitory effect of halocyamine, an antimicrobial substance from ascidian hemocytes, on the growth of fish viruses and marine bacteria. | halocyamine a, an antimicrobial substance isolated from hemocytes of the solitary ascidian halocynthia roretzi, inhibited in vitro the growth of fish rna viruses (infectious hematopoietic necrosis virus and infectious pancreatic necrosis virus). pretreatment of rna virus with halocyamine a reduced the infectivity of the virus toward host cells. the growth of marine bacteria, achromobacter aquamarinus and pseudomonas perfectomarinus, was also inhibited by halocyamine a but that of alteromonas put ... | 1990 | 2121517 |
| regulation and energization of nitrate transport in a halophilic pseudomonas stutzeri. | nitrate transport and its regulation by oxygen was studied in denitrifying halophilic pseudomonas stutzeri, strain zobell, and a tn-5 transposon nitrite reductase mutant of this organism. the rate of nitrate transport was found to be 130 nanomoles nitrate min-1 mg protein-1 and 150 nanomoles nitrate min-1 mg protein-1 in the wildtype and the nitrite reductase mutant respectively as compared to 26.4 nanomoles nitrate min-1 mg protein-1 in a non-halophilic pseudomonas stutzeri. asparagine was foun ... | 1990 | 2154208 |
| multifrequency epr evidence for a bimetallic center at the cua site in cytochrome c oxidase. | multifrequency electron paramagnetic resonance (epr) spectra of the cu(ii) site in bovine heart cytochrome c oxidase (cox) and nitrous oxide reductase (n2or) from pseudomonas stutzeri confirm the existence of cu-cu interaction in both enzymes. c-band (4.5 ghz) proves to be a particularly good frequency complementing the spectra of cox and n2or recorded at 2.4 and 3.5 ghz. both the high and low field region of the epr spectra show the presence of a well-resolved 7-line pattern consistent with the ... | 1990 | 2166686 |
| nitrous oxide reductase from denitrifying pseudomonas stutzeri. genes for copper-processing and properties of the deduced products, including a new member of the family of atp/gtp-binding proteins. | nitrous oxide (n2o) respiration by the denitrifying bacterium pseudomonas stutzeri requires the synthesis of the multi-copper enzyme n2o reductase, nosz, the structural gene for this enzyme, is part of a dna region of approximately 8 kbp that carries several essential genes. insertional mutagenesis of the region downstream of nosz generates apoenzyme-synthesizing strains, which argues for the existence of functions for copper acquisition or copper processing, or both, for n2o reductase. the rele ... | 1990 | 2170125 |
| the cellular location and specificity of bacterial cytochrome c peroxidases. | the locations of cytochrome c peroxidase and catalase activities in the two gram-negative bacteria pseudomonas stutzeri (n.c.i.b. 9721) and paracoccus denitrificans (n.c.i.b. 8944) were investigated by the production of spheroplasts. in both species the cytochrome c peroxidase was predominantly periplasmic: 92% of total activity in ps. stutzeri and 98% of nonmembrane-bound activity in pa. denitrificans were found in this cellular compartment. in contrast, the catalase was mostly in the cytoplasm ... | 1990 | 2173903 |
| isolation of bacterial strains, which hydrolyze glycyrrhizin and produce glycyrrhezic acid, from soil. | we isolated eight bacterial strains which could hydrolyze glycyrrhizin to glycyrrhezic acid. the bacterial strains were identified as three strains of pseudomonas saccharophila, two of plesiomonas sp., one of pseudomonas stutzeri, one of klebsiella pneumoniae subsp. ozaenae and one of kluyvera ascorbata. their capacity for the conversion of glycyrrhizin to glycyrrhezic acid was assayed by high performance liquid chromatography. p. saccharophila 11 was the most effective among the eight strains. ... | 1990 | 2231267 |
| natural genetic transformation of pseudomonas stutzeri by sand-adsorbed dna. | in a soil/sediment model system we have shown recently that a gram-positive bacterium with natural competence (bacillus subtilis) can take up transforming dna adsorbed to sand minerals. here we examined whether also a naturally transformable soil bacterium of the gram-negative pseudomonad (pseudomonas stutzeri) can be transformed by mineral-associated dna. for these studies the transformation protocol of this species was further improved and characterized. the peak of competence during growth of ... | 1990 | 2244790 |
| pseudomonas stutzeri synthetic vascular graft infection. | | 1990 | 2318662 |
| steady-state nitric oxide concentrations during denitrification. | three species of denitrifying bacteria, paracoccus denitrificans, pseudomonas stutzeri strain jm300, and achromobacter cycloclastes, were allowed to reduce nitrate or nitrite in anaerobic, closed vials while the equilibration of gases between aqueous and gas phases was facilitated by vigorous stirring. the gas phase was sampled and analyzed for no with use of a chemiluminescence detector calibrated against bottled no standards or against no produced by the nitrite-iodide reaction. [noaq] was inf ... | 1990 | 2365685 |
| monoclonal antibodies that recognize a specific surface antigen of treponema denticola. | spirochetes have been implicated as potential etiologic agents of periodontitis in humans. murine monoclonal antibodies (mabs) specific for a serogroup of treponema denticola, an oral spirochete, were developed and characterized in this study. antibodies secreted by clone iaa11 were judged to be the most useful, since they were able to detect 8 of 15 t. denticola strains. this mab consisted of an immunoglobulin g3 heavy chain and a kappa light chain. mab iaa11 was found to react with an epitope ... | 1988 | 2447020 |
| enzymatic synthesis of p-nitrophenyl alpha-maltopentaoside in an aqueous-methanol solvent system by maltotetraose-forming amylase: a substrate for human amylase in serum. | transglycosylation from maltopentaose to the 4-position of p-nitrophenyl alpha-glucoside was efficiently induced through the use of maltotetraose-forming amylase from pseudomonas stutzeri in an aqueous solution containing methanol at a high concentration. the enzyme specifically formed p-nitrophenyl alpha-maltopentaoside (12% of the enzyme-catalyzed net decrease of maltopentaose) from maltopentaose as a donor and p-nitrophenyl alpha-glucoside as an acceptor. the rate of the transglycosylation de ... | 1988 | 2459114 |
| properties of a pseudomonas stutzeri outer membrane channel-forming protein (nosa) required for production of copper-containing n2o reductase. | a protein (nosa) in the outer membrane of pseudomonas stutzeri that is required for copper to be inserted into n2o reductase has been extracted and purified to homogeneity. the purified protein could form channels in black lipid bilayers. like n2o reductase, nosa contained copper and was only made anaerobically. in contrast to n2o reductase, its synthesis was repressed by exogenous copper (but not by mn, co, ni, zn, or fe). also in contrast to n2o reductase, nosa homologs were not immunologicall ... | 1989 | 2467899 |
| silver accumulation in pseudomonas stutzeri ag259. | silver toxicity to pseudomonas stutzeri ag259 was strongly dependent on the nacl concentration in the medium, which reduced the availability of ag+ by precipitation as agcl. accumulation of ag by growing cultures was low being less than or equal to 7.5 nmol (mg dry mass)-1 over all treatments examined. the presence of nacl in the growth medium did not markedly affect the amounts of ag accumulated by the cells but influenced toxicity as manifest by a lag period which was greatest at low nacl conc ... | 1989 | 2490072 |
| [evaluation of the treatment with oral ciprofloxacin: experience with 24 patients]. | we have evaluated the clinical effectiveness and possible side effects of oral ciprofloxacin therapy in 24 patients (11 females and 13 males). two episodes from overall 25 infections were excluded of clinical and microbiological evaluation but were assessed regarding possible side effects. in most patients, the clinical condition was stable at the beginning of therapy. only 3 patients had nonfatal diseases. seventeen episodes were urinary tract infections (four of them with concomitant bacteremi ... | 1989 | 2490722 |
| selective medium for pseudomonas aeruginosa that uses 1,10-phenanthroline as the selective agent. | the mic of 1,10-phenanthroline for 35 pseudomonas aeruginosa strains was 128 micrograms/ml, whereas 32 micrograms or less per ml inhibited all other microorganisms tested. on the basis of these results, a selective agar for p. aeruginosa which contained 15 g of trypticase soy broth (bbl microbiology systems), 15 g of agar, and 0.1 g of phenanthroline per liter was formulated. forty-four p. aeruginosa strains yielded a mean efficiency of plating on this medium of 79% of the counts obtained on try ... | 1989 | 2515804 |
| nitrous oxide reductase from pseudomonas stutzeri. redox properties and spectroscopic characterization of different forms of the multicopper enzyme. | the oxidation-reduction and spectroscopic properties of various forms of nitrous oxide reductase from pseudomonas stutzeri were investigated. the high-activity form i of the enzyme (purple, 8 cu, mr 140,000) was reduced by a large variety of cationic, anionic and photochemically generated agents. the blue form iii was the only product found in these experiments under anaerobic conditions. reductive (dithionite) and oxidative (ferricyanide) titrations showed that the conversion of the purple form ... | 1989 | 2536326 |
| cytochrome c peroxidase activity of a protease-modified form of cytochrome c-552 from the denitrifying bacterium pseudomonas perfectomarina. | protease activity present in aerobically grown cells of pseudomonas perfectomarina, protease apparently copurified with cytochrome c-552, and trypsin achieved a limited proteolysis of the diheme cytochrome c-552. that partial lysis conferred cytochrome c peroxidase activity upon cytochrome c-552. the removal of a 4000-da peptide explains the structural changes in the cytochrome c-552 molecule that resulted in the appearance of both cytochrome c peroxidase activity (with optimum activity at ph 8. ... | 1989 | 2539041 |
| formation of the n-n bond from nitric oxide by a membrane-bound cytochrome bc complex of nitrate-respiring (denitrifying) pseudomonas stutzeri. | nitric oxide (no) reductase was solubilized by triton x-100 from the membrane fraction of pseudomonas stutzeri zobell and purified 100-fold to apparent electrophoretic homogeneity. the enzyme consisted of two polypeptides of mr 38,000 and 17,000 associated with heme b and heme c, respectively. absorption maxima of the reduced complex were at 420.5, 522.5, and 552.5 nm, with a shoulder at 560 nm. the electron paramagnetic resonance spectrum was characteristic of high- and low-spin ferric heme pro ... | 1989 | 2542222 |
| pseudomonas stutzeri n2o reductase contains cua-type sites. | n2o reductase (n2o----n2) is the terminal enzyme in the energy-conserving denitrification pathway of soil and marine denitrifying bacteria. the protein is composed of two identical subunits and contains eight copper ions per enzyme molecule. the magnetic circular dichroism spectrum of resting (oxidized) n2o reductase is strikingly similar to the magnetic circular dichroism spectrum of the cua site in mammalian cytochrome c oxidase [greenwood, c., hull, b. c., barber, d., eglinton, d. g. & thomso ... | 1989 | 2542963 |
| the role of cytochrome c4 in bacterial respiration. cellular location and selective removal from membranes. | the cellular location of cytochrome c4 in pseudomonas stutzeri and azotobacter vinelandii was investigated by the production of spheroplasts. soluble cytochrome c4 was found to be located in the periplasm in both organisms. the remaining cytochrome c4 was membrane-bound. the orientation of this membrane-bound cytochrome c4 fraction was investigated by proteolysis of the cytochrome on intact spheroplasts. in p. stutzeri, 78% of the membrane-bound cytochrome c4 could be proteolysed, whilst 82% of ... | 1989 | 2554884 |
| formate dependent nitrate and nitrite reduction to ammonia by citrobacter freundii and competition with denitrifying bacteria. | citrobacter freundii, paracoccus denitrificans and pseudomonas stutzeri were grown either singly or in mixed culture in anaerobic nitrate or nitrite limited chemostats with formate and/or succinate as electron donors and carbon sources. c. freundii reduced nitrate or nitrite stoichiometrically to ammonia. maximum molar growth yields for nitrate (nitrite) were 15.3 (9.9) g/mol for c. freundii on formate with succinate as carbon source, 15.3 (9.5) g/mol for ps. stutzeri on succinate and 32.3 (20.4 ... | 1989 | 2619287 |
| immunological identification and distribution of dissimilatory heme cd1 and nonheme copper nitrite reductases in denitrifying bacteria. | polyclonal antibodies were used to identify heme or copper nitrite reductases in the following groups: 23 taxonomically diverse denitrifiers from culture collections, 100 numerically dominant denitrifiers from geographically diverse environments, and 51 denitrifiers from a culture collection not selected for denitrification. antisera were raised against heme nitrite reductases from pseudomonas aeruginosa and pseudomonas stutzeri and against copper nitrite reductase from achromobacter cycloclaste ... | 1989 | 2624465 |
| a nosa-specific bacteriophage can be used to select denitrification-defective mutants of pseudomonas stutzeri. | phi ps5, a double-stranded dna bacteriophage of pseudomonas stutzeri jm604 that adsorbs specifically to the outer-membrane protein nosa, was isolated from stagnant irrigation ditch water. mutant strains that do not produce nosa are resistant to phi ps5 and cannot grow anaerobically with n2o as the sole electron acceptor. phi ps5 did not adsorb to nosa mutants and adsorption to the wild-type strain was reduced when cells were grown with a high concentration of copper, a condition that represses t ... | 1989 | 2632664 |
| identification and dna sequencing of a new plasmid (ppst1) in pseudomonas stutzeri mo-19. | a cryptic plasmid, ppst1, was isolated from pseudomonas stutzeri mo-19 and its complete nucleotide sequence was determined. this plasmid consisted of 1446 bp and could encode a putative polypeptide of 152 amino acid residues (orf1) in an open reading frame. the putative protein contained a sequence homologous to the sequences found in dna-binding sites. | 1989 | 2633209 |
| cloning and nucleotide sequence of the gene (amyp) for maltotetraose-forming amylase from pseudomonas stutzeri mo-19. | the gene (amyp) coding for maltotetraose-forming amylase (exo-maltotetraohydrolase) of pseudomonas stutzeri mo-19 was cloned. its nucleotide sequence contained an open reading frame coding for a precursor (547 amino acid residues) of secreted amylase. the precursor had a signal peptide of 21 amino acid residues at its amino terminus. an extract of escherichia coli carrying the cloned amyp had amylolytic activity with the same mode of action as the extracellular exo-maltotetraohydrolase obtained ... | 1989 | 2646279 |
| subcutaneous granuloma caused by phialophora richardsiae: case report and review of the literature. | a phaeomycotic cyst in a 47-year-old man, caused by phialophora richardsiae, was treated successfully by excision. a critical review of the literature indicates that the pathological course of p. richardsiae infections usually follows a similar pattern to that of the present case, viz. generation of a well-defined and limited nodule following traumatic subcutaneous introduction of the fungus. the nodule typically accumulates a viscous yellow fluid. the infection remains localized, often being en ... | 1989 | 2668753 |
| expression of denitrification enzymes in response to the dissolved oxygen level and respiratory substrate in continuous culture of pseudomonas stutzeri. | the onset and cessation of the synthesis of denitrification enzymes of pseudomonas stutzeri were investigated by using continuous culture and defined dissolved oxygen levels covering the full range of transition from air saturation to complete anaerobiosis. expression of nitrate reductase, nitrite reductase (cytochrome cd1), and n2o reductase was controlled by discrete oxygen levels and by the nature of the nitrogenous oxide available for respiration. n2o reductase was synthesized constitutively ... | 1989 | 2764573 |
| microbiological degradation of quinoline by pseudomonas stutzeri: the coumarin pathway of quinoline catabolism. | a gram-negative, oxidase positive, polar flagellated rod, characterised as pseudomonas stutzeri, has been isolated from sewage by enrichment culture on quinoline. the organism utilizes quinoline as the sole source of carbon, nitrogen and energy, and liberates uv absorbing and phenolic metabolites during its growth on quinoline. 2-hydroxyquinoline, 2,8-dihydroxyquinoline, 8-hydroxycoumarin and 2,3-dihydroxyphenylpropionic acid have been isolated as the transformation products of quinoline by this ... | 1989 | 2770558 |
| physical mapping of transposon tn5 insertions defines a gene cluster functional in nitrous oxide respiration by pseudomonas stutzeri. | by transposon tn5 mutagenesis, 19 strains of pseudomonas stutzeri were acquired that had defects in nitrous oxide respiration (nos- phenotype). a physical map of the mutants showed nearly random tn5 insertions into genomic dna within a single region ca. 8 kilobases long. mutants were characterized immunochemically, enzymatically, and chemically. several functions related to the synthesis and regulation of nitrous oxide reductase were associated with this dna region, indicating that in p. stutzer ... | 1987 | 2820935 |
| plasmid pcbi carries genes for anaerobic benzoate catabolism in alcaligenes xylosoxidans subsp. denitrificans pn-1. | pseudomonas sp. strain pn-1 is reclassified as alcaligenes xylosoxidans subsp. denitrificans pn-1. strain pn-1 is a gram-negative, rod-shaped organism, is motile by means of lateral flagella, is oxidase positive, and does not ferment sugars. plasmid pcbi, carrying genes for the anaerobic degradation of benzoate in strain pn-1, is 17.4 kilobase pairs in length and is transmissible to a number of denitrifying pseudomonas aeruginosa and pseudomonas stutzeri strains. a restriction endonuclease map w ... | 1987 | 2822651 |
| interpretation of effects of ph on rate constants for the oxidation of three ferrocytochromes c-551 with [fe(cn)6]3- and [co(phen)3]3+, and assignment of pka values. | effects of ph on second-order rate constants, k (25 degrees c), have been determined for the [fe(cn)6]3- and [co(phen)3]3+ oxidations of ferrocytochrome c-551 from pseudomonas aeruginosa, pseudomonas stutzeri, and azotobacter vinelandii. for each oxidant similar directional trends are observed. with [fe(cn)6]3-, rate constants over the ph 4-9.5 range first decrease, and then increase to plateau ph approximately equal to 9 k values of 0.96.10(5), 4.4.10(5) and 1.05.10(5) m-1.s-1, respectively. wi ... | 1988 | 2827782 |
| electron self-exchange in pseudomonas cytochromes. | electron self-exchange has been measured by an nmr technique for cytochromes c551 from pseudomonas aeruginosa and pseudomonas stutzeri. the rate for p. aeruginosa cyt c551 is 1.2 x 10(7) m-1 s-1 at 40 degrees c in 50 mm phosphate at ph 7. for p. stutzeri, under the same conditions, the rate is 4 x 10(7) m-1 s-1. for both cytochromes, the rate was independent of ionic strength up to 0.5 m in added nac1, the enthalpy of activation was 20 +/- 4 kcal mol-1, and the entropy of activation was 38 +/- 1 ... | 1988 | 2829889 |
| defects in cytochrome cd1-dependent nitrite respiration of transposon tn5-induced mutants from pseudomonas stutzeri. | mutants with defective respiratory nitrite utilization (nir- phenotype) were obtained by transposon tn5 insertion into genomic dna of the zobell strain of pseudomonas stutzeri. three representative mutants were characterized with respect to their activities of nitrite and nitric oxide reduction, cytochrome cd1 content, and pattern of soluble c-type cytochromes. mutant strain mk201 overproduced cytochrome c552 about fourfold by comparison with the wild type, but possessed an in vitro functional c ... | 1988 | 2839127 |
| free and membrane-bound forms of bacterial cytochrome c4. | cytochrome c4 was isolated from cells of pseudomonas aeruginosa, pseudomonas stutzeri and azotobacter vinelandii. the dihaem nature, mr of approx. 20,000 and ferrohaem spectra in the region of the alpha- and beta-peaks define this family of cytochromes c. the behaviour of the holocytochromes in sds was atypical, but removal of the haem groups resulted in a normal migration. in all three organisms most of the cytochrome c4 was tightly bound to the membrane, but some free cytochrome was detected. ... | 1988 | 2843169 |
| separate binding sites for antimycin and mucidin in the respiratory chain of the bacterium paracoccus denitrificans and their occurrence in other denitrificans bacteria. | by means of the method of fluorimetric titration it has been shown that mucidin does not affect the attachment of antimycin to membranes from anaerobically grown paracoccus denitrificans. the fluorimetric titration with antimycin can be used in the determination of the amount of the cytochrome bc1 complex in the membrane. in cells inhibited with antimycin, the oxidation of cytochromes c was accompanied by the reduction of cytochrome b; in the presence of mucidin this effect did not take place. t ... | 1988 | 2844159 |
| nitric oxide complex of cytochrome c' in cells of denitrifying bacteria. | electron paramagnetic resonance (epr) spectra at 77k have been measured for the dried cells of three groups of chemoheterotrophic bacteria cultured in the presence of nitrate: i, denitrifying bacteria containing cytochrome c' (alcaligenes sp. ncib 11015, achromobacter xylosoxidans gifu 543); ii, denitrifying bacteria in which cytochrome c' has not been found (alcaligenes faecalis iam 1015, bacillus firmus nias 237, pseudomonas stutzeri atcc 17641); iii, non-denitrifying bacterium containing cyto ... | 1988 | 2844743 |
| a spectrophotometric assay for dissimilatory nitrite reductases. | a spectrophotometric assay for dissimilatory nitrite reductases has been developed utilizing mammalian cytochrome c (equine heart) as reductant and spectrophotometric agent. the copper-containing nitrite reductase from achromobacter cycloclastes has been shown to have apparent km's for reduced cytochrome c and nitrite of 86 +/- 5 and 5.63 +/- 0.03 microm, respectively. the heme cd-containing enzyme from pseudomonas stutzeri was shown to have apparent km's for reduced cytochrome c and nitrite of ... | 1988 | 2847585 |
| cytochrome components of nitrate- and sulfate-respiring desulfovibrio desulfuricans atcc 27774. | three multiheme c-type cytochromes--the tetraheme cytochrome c3 (molecular weight [mw] 13,500), a dodecaheme cytochrome c (mw 40,800), and a "split-soret" cytochrome c (mw 51,540), which is a dimer with 2 hemes per subunit (mw 26,300)--were isolated from the soluble fraction of desulfovibrio desulfuricans (atcc 27774) grown under nitrate- or sulfate-respiring conditions. two of them, the dodecaheme and the split-soret cytochromes, showed no similarities to any of the c-type cytochromes isolated ... | 1988 | 2848008 |
| the cupric site in nitrous oxide reductase contains a mixed-valence [cu(ii),cu(i)] binuclear center: a multifrequency electron paramagnetic resonance investigation. | multifrequency electron paramagnetic resonance (epr) spectra of the cu(ii) site in nitrous oxide reductase (n2or) from pseudomonas stutzeri confirm the assignment of the low field g value at 2.18 consistent with the seven line pattern observed at 9.31 ghz, 10 k. s-band spectra at 20 k are better resolved than the x-band spectra recorded at 10 k. the features observed at 2.4, 3.4, 9.31 and 35 ghz are explained by a mixed-valence [cu(1.5)..cu(1.5)] s = 1/2 species with the unpaired electron deloca ... | 1988 | 2849565 |
| a novel vector allowing the expression of genes in a wide range of gram-negative bacteria. | the construction and use of a novel vector allowing the expression of genes in a wide range of gram-negative bacteria is described. the vector utilizes the regulatory region from is50. the 70-bp promoter region was isolated from one of the terminal inverted repeats of tn5 by creating ecori and sa/i or psti restriction sites by in vitro mutagenesis. this 70-bp region was shown to direct the expression of cat and lacz genes in different bacterial genera including alcaligenes, enterobacter cloacae, ... | 1988 | 2853690 |
| nitric oxide and nitrous oxide production and cycling during dissimilatory nitrite reduction by pseudomonas perfectomarina. | the denitrifier pseudomonas perfectomarina reduced nitrite under conditions of kinetic competition between cells and gas sparging for extracellular dissolved nitric and nitrous oxides, noaq and n2oaq, in a chemically defined marine medium. time courses of nitrite reduction and nog and n2og alpha removal were integrated to give nog and n2og yields. at high sparging rates, the nog yield was greater than 50% of nitrite-n reduced, and the yield of nog + n2og was approximately 75%. hence interrupted ... | 1989 | 2925629 |
| complexity in the redox titration of the dihaem cytochrome c4. | redox titration of the dihaem, two domain cytochromes c4 from pseudomonas aeruginosa, pseudomonas stutzeri and azotobacter vinelandii showed complex behaviour indicative of the presence of two redox components. in the case of the p. stutzeri cytochrome c4, two spectroscopically distinct components were present during the redox titration. in contrast, cytochrome c-554(548) from a halophilic paracoccus species is a stable dimer of a monohaem cytochrome which shows close homology to cytochrome c4, ... | 1985 | 2990552 |
| isolation and characterization of transposon tn5-induced mutants of pseudomonas perfectomarina defective in nitrous oxide respiration. | transposon (tn5) mutagenesis of pseudomonas perfectomarina with the plasmid psup2021 [(pbr325-mob(rp4))::tn5] and the chromosomally integrated rp4 plasmid in escherichia coli as the donor, produced three distinct groups of mutants that were defective in nitrous oxide respiration. one group of mutants lacked the structural protein of n2o reductase, the second synthesized a copper-free apoprotein; and a third group expressed a low level of intact enzyme. the mutants provided evidence for n2o being ... | 1985 | 2993252 |
| nitrous oxide reduction by members of the family rhodospirillaceae and the nitrous oxide reductase of rhodopseudomonas capsulata. | after growth in the absence of nitrogenous oxides under anaerobic phototrophic conditions, several strains of rhodopseudomonas capsulata were shown to possess a nitrous oxide reductase activity. the enzyme responsible for this activity had a periplasmic location and resembled a nitrous oxide reductase purified from pseudomonas perfectomarinus. electron flow to nitrous oxide reductase was coupled to generation of a membrane potential and inhibited by rotenone but not antimycin. it is suggested th ... | 1985 | 2997133 |
| nitrous oxide reductase from denitrifying pseudomonas perfectomarina. purification and properties of a novel multicopper enzyme. | nitrous oxide reductase from the denitrifying bacterium pseudomonas perfectomarina has been isolated and purified to homogeneity. the enzyme contained about eight copper atoms/120 kda and was composed of two presumably identical subunits. the isoelectric point was 5.1. several spectroscopically distinct forms of the enzyme were identified. a 'pink' form of the enzyme was obtained when the purification was done aerobically. the specific activity of this species was around 30 nkat/mg protein as me ... | 1985 | 3000778 |